E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 862 |
| AccII | CGCG | 1 cut(s) | 42 |
| AciI | CCGC | 3 cut(s) | 40, 42, 676 |
| AclWI | GGATC | 1 cut(s) | 23 |
| AcsI | RAATTY | 1 cut(s) | 836 |
| AcuI | CTGAAG | 1 cut(s) | 336 |
| AfaI | GTAC | 6 cut(s) | 11, 70, 580, 605, 709, 859 |
| AflIII | ACRYGT | 1 cut(s) | 656 |
| AgsI | TTSAA | 5 cut(s) | 137, 737, 796, 891, 925 |
| AjnI | CCWGG | 2 cut(s) | 80, 397 |
| AluBI | AGCT | 6 cut(s) | 62, 356, 476, 665, 765, 780 |
| AluI | AGCT | 6 cut(s) | 62, 356, 476, 665, 765, 780 |
| Alw26I | GTCTC | 1 cut(s) | 849 |
| AlwI | GGATC | 1 cut(s) | 23 |
| AoxI | GGCC | 2 cut(s) | 23, 43 |
| ApeKI | GCWGC | 2 cut(s) | 489, 609 |
| ApoI | RAATTY | 1 cut(s) | 836 |
| AspLEI | GCGC | 1 cut(s) | 966 |
| AspS9I | GGNCC | 2 cut(s) | 24, 44 |
| AsuNHI | GCTAGC | 1 cut(s) | 356 |
| BbsI | GAAGAC | 3 cut(s) | 157, 297, 439 |
| BbvI | GCAGC | 2 cut(s) | 476, 596 |
| BccI | CCATC | 4 cut(s) | 191, 277, 322, 563 |
| BceAI | ACGGC | 1 cut(s) | 622 |
| BciT130I | CCWGG | 2 cut(s) | 82, 399 |
| BclI | TGATCA | 3 cut(s) | 262, 268, 457 |
| BcoDI | GTCTC | 1 cut(s) | 849 |
| BfaI | CTAG | 2 cut(s) | 357, 777 |
| BfmI | CTRYAG | 1 cut(s) | 869 |
| BfuAI | ACCTGC | 1 cut(s) | 862 |
| BisI | GCNGC | 4 cut(s) | 43, 490, 610, 676 |
| BlsI | GCNGC | 4 cut(s) | 44, 491, 611, 677 |
| BmcAI | AGTACT | 1 cut(s) | 859 |
| Bme1390I | CCNGG | 2 cut(s) | 82, 399 |
| BmgT120I | GGNCC | 2 cut(s) | 24, 44 |
| BmiI | GGNNCC | 1 cut(s) | 538 |
| BmrFI | CCNGG | 2 cut(s) | 82, 399 |
| BmsI | GCATC | 1 cut(s) | 76 |
| BmtI | GCTAGC | 1 cut(s) | 360 |
| BpiI | GAAGAC | 3 cut(s) | 157, 297, 439 |
| BpmI | CTGGAG | 1 cut(s) | 42 |
| BsaBI | GATNNNNATC | 2 cut(s) | 261, 267 |
| BsaI | GGTCTC | 1 cut(s) | 849 |
| BsaJI | CCNNGG | 4 cut(s) | 40, 81, 155, 397 |
| BsaWI | WCCGGW | 1 cut(s) | 12 |
| Bse1I | ACTGG | 1 cut(s) | 451 |
| Bse8I | GATNNNNATC | 2 cut(s) | 261, 267 |
| BseBI | CCWGG | 2 cut(s) | 82, 399 |
| BseDI | CCNNGG | 4 cut(s) | 40, 81, 155, 397 |
| BseGI | GGATG | 3 cut(s) | 18, 91, 292 |
| BseJI | GATNNNNATC | 2 cut(s) | 261, 267 |
| BseMII | CTCAG | 1 cut(s) | 104 |
| BseNI | ACTGG | 1 cut(s) | 451 |
| BseRI | GAGGAG | 1 cut(s) | 293 |
| BseXI | GCAGC | 2 cut(s) | 476, 596 |
| BsgI | GTGCAG | 2 cut(s) | 475, 511 |
| Bsh1236I | CGCG | 1 cut(s) | 42 |
| BshFI | GGCC | 2 cut(s) | 25, 45 |
| BsiSI | CCGG | 2 cut(s) | 13, 22 |
| BsmAI | GTCTC | 1 cut(s) | 849 |
| BsmI | GAATGC | 1 cut(s) | 371 |
| BsnI | GGCC | 2 cut(s) | 25, 45 |
| Bso31I | GGTCTC | 1 cut(s) | 849 |
| Bsp143I | GATC | 5 cut(s) | 15, 262, 268, 457, 802 |
| BspACI | CCGC | 3 cut(s) | 40, 42, 676 |
| BspANI | GGCC | 2 cut(s) | 25, 45 |
| BspCNI | CTCAG | 1 cut(s) | 103 |
| BspFNI | CGCG | 1 cut(s) | 42 |
| BspHI | TCATGA | 2 cut(s) | 265, 271 |
| BspLI | GGNNCC | 1 cut(s) | 538 |
| BspMAI | CTGCAG | 1 cut(s) | 873 |
| BspMI | ACCTGC | 1 cut(s) | 862 |
| BspOI | GCTAGC | 1 cut(s) | 360 |
| BspPI | GGATC | 1 cut(s) | 23 |
| BspTNI | GGTCTC | 1 cut(s) | 849 |
| BsrI | ACTGG | 1 cut(s) | 451 |
| BssECI | CCNNGG | 4 cut(s) | 40, 81, 155, 397 |
| BssMI | GATC | 5 cut(s) | 15, 262, 268, 457, 802 |
| Bst2UI | CCWGG | 2 cut(s) | 82, 399 |
| Bst4CI | ACNGT | 2 cut(s) | 439, 760 |
| Bst6I | CTCTTC | 1 cut(s) | 270 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 366 |
| BstC8I | GCNNGC | 3 cut(s) | 358, 478, 869 |
| BstDEI | CTNAG | 1 cut(s) | 90 |
| BstDSI | CCRYGG | 1 cut(s) | 40 |
| BstF5I | GGATG | 3 cut(s) | 18, 91, 292 |
| BstFNI | CGCG | 1 cut(s) | 42 |
| BstHHI | GCGC | 1 cut(s) | 966 |
| BstKTI | GATC | 5 cut(s) | 18, 265, 271, 460, 805 |
| BstMAI | GTCTC | 1 cut(s) | 849 |
| BstMBI | GATC | 5 cut(s) | 15, 262, 268, 457, 802 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 366, 420, 486, 534 |
| BstNI | CCWGG | 2 cut(s) | 82, 399 |
| BstNSI | RCATGY | 1 cut(s) | 660 |
| BstSCI | CCNGG | 2 cut(s) | 80, 397 |
| BstSFI | CTRYAG | 1 cut(s) | 869 |
| BstUI | CGCG | 1 cut(s) | 42 |
| BstV1I | GCAGC | 2 cut(s) | 476, 596 |
| BstV2I | GAAGAC | 3 cut(s) | 157, 297, 439 |
| BsuRI | GGCC | 2 cut(s) | 25, 45 |
| BtgI | CCRYGG | 1 cut(s) | 40 |
| BtsCI | GGATG | 3 cut(s) | 18, 91, 292 |
| BtsIMutI | CAGTG | 1 cut(s) | 458 |
| BveI | ACCTGC | 1 cut(s) | 862 |
| Cac8I | GCNNGC | 3 cut(s) | 358, 478, 869 |
| CciI | TCATGA | 2 cut(s) | 265, 271 |
| CfoI | GCGC | 1 cut(s) | 966 |
| Cfr13I | GGNCC | 2 cut(s) | 24, 44 |
| Cfr42I | CCGCGG | 1 cut(s) | 43 |
| Csp6I | GTAC | 6 cut(s) | 10, 69, 579, 604, 708, 858 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 913, 948 |
| CviAII | CATG | 6 cut(s) | 266, 272, 647, 657, 784, 912 |
| CviQI | GTAC | 6 cut(s) | 10, 69, 579, 604, 708, 858 |
| DdeI | CTNAG | 1 cut(s) | 90 |
| DpnI | GATC | 5 cut(s) | 17, 264, 270, 459, 804 |
| DpnII | GATC | 5 cut(s) | 15, 262, 268, 457, 802 |
| Eam1104I | CTCTTC | 1 cut(s) | 270 |
| EarI | CTCTTC | 1 cut(s) | 270 |
| Eco31I | GGTCTC | 1 cut(s) | 849 |
| Eco57I | CTGAAG | 1 cut(s) | 336 |
| EcoRII | CCWGG | 2 cut(s) | 80, 397 |
| FaeI | CATG | 6 cut(s) | 269, 275, 650, 660, 787, 915 |
| FatI | CATG | 6 cut(s) | 265, 271, 646, 656, 783, 911 |
| FbaI | TGATCA | 3 cut(s) | 262, 268, 457 |
| Fnu4HI | GCNGC | 4 cut(s) | 43, 490, 610, 676 |
| FokI | GGATG | 3 cut(s) | 5, 98, 299 |
| Fsp4HI | GCNGC | 4 cut(s) | 43, 490, 610, 676 |
| FspBI | CTAG | 2 cut(s) | 357, 777 |
| GlaI | GCGC | 1 cut(s) | 965 |
| GluI | GCNGC | 4 cut(s) | 43, 490, 610, 676 |
| GsuI | CTGGAG | 1 cut(s) | 42 |
| HaeIII | GGCC | 2 cut(s) | 25, 45 |
| HapII | CCGG | 2 cut(s) | 13, 22 |
| HhaI | GCGC | 1 cut(s) | 966 |
| Hin1II | CATG | 6 cut(s) | 269, 275, 650, 660, 787, 915 |
| Hin6I | GCGC | 1 cut(s) | 964 |
| HinP1I | GCGC | 1 cut(s) | 964 |
| HinfI | GANTC | 4 cut(s) | 50, 117, 179, 572 |
| HpaII | CCGG | 2 cut(s) | 13, 22 |
| Hpy166II | GTNNAC | 1 cut(s) | 428 |
| Hpy188I | TCNGA | 4 cut(s) | 93, 124, 184, 849 |
| Hpy188III | TCNNGA | 7 cut(s) | 114, 266, 272, 559, 692, 891, 943 |
| Hpy8I | GTNNAC | 1 cut(s) | 428 |
| HpyAV | CCTTC | 2 cut(s) | 179, 897 |
| HpyCH4III | ACNGT | 2 cut(s) | 439, 760 |
| HpyCH4V | TGCA | 5 cut(s) | 492, 528, 591, 612, 871 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 366, 420, 486, 534 |
| HpyF3I | CTNAG | 1 cut(s) | 90 |
| Hsp92II | CATG | 6 cut(s) | 269, 275, 650, 660, 787, 915 |
| HspAI | GCGC | 1 cut(s) | 964 |
| Ksp22I | TGATCA | 3 cut(s) | 262, 268, 457 |
| KspI | CCGCGG | 1 cut(s) | 43 |
| Kzo9I | GATC | 5 cut(s) | 15, 262, 268, 457, 802 |
| LmnI | GCTCC | 2 cut(s) | 485, 542 |
| Lsp1109I | GCAGC | 2 cut(s) | 476, 596 |
| LweI | GCATC | 1 cut(s) | 76 |
| MaeI | CTAG | 2 cut(s) | 357, 777 |
| MaeIII | GTNAC | 2 cut(s) | 622, 754 |
| MalI | GATC | 5 cut(s) | 17, 264, 270, 459, 804 |
| MboI | GATC | 5 cut(s) | 15, 262, 268, 457, 802 |
| MluCI | AATT | 2 cut(s) | 417, 836 |
| MnlI | CCTC | 8 cut(s) | 37, 95, 165, 166, 184, 271, 731, 781 |
| MseI | TTAA | 5 cut(s) | 146, 167, 389, 876, 905 |
| MslI | CAYNNNNRTG | 1 cut(s) | 270 |
| MspA1I | CMGCKG | 2 cut(s) | 42, 62 |
| MspI | CCGG | 2 cut(s) | 13, 22 |
| MspR9I | CCNGG | 2 cut(s) | 82, 399 |
| Mva1269I | GAATGC | 1 cut(s) | 371 |
| MvaI | CCWGG | 2 cut(s) | 82, 399 |
| MvnI | CGCG | 1 cut(s) | 42 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 366, 420, 486, 534 |
| NdeII | GATC | 5 cut(s) | 15, 262, 268, 457, 802 |
| NheI | GCTAGC | 1 cut(s) | 356 |
| NlaIII | CATG | 6 cut(s) | 269, 275, 650, 660, 787, 915 |
| NlaIV | GGNNCC | 1 cut(s) | 538 |
| NmeAIII | GCCGAG | 1 cut(s) | 575 |
| NmuCI | GTSAC | 2 cut(s) | 622, 754 |
| NspI | RCATGY | 1 cut(s) | 660 |
| PagI | TCATGA | 2 cut(s) | 265, 271 |
| PciI | ACATGT | 1 cut(s) | 656 |
| PctI | GAATGC | 1 cut(s) | 371 |
| PfeI | GAWTC | 4 cut(s) | 50, 117, 179, 572 |
| PkrI | GCNGC | 4 cut(s) | 44, 491, 611, 677 |
| PscI | ACATGT | 1 cut(s) | 656 |
| Psp6I | CCWGG | 2 cut(s) | 80, 397 |
| PspGI | CCWGG | 2 cut(s) | 80, 397 |
| PspN4I | GGNNCC | 1 cut(s) | 538 |
| PspPI | GGNCC | 2 cut(s) | 24, 44 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 61, 93 |
| PstI | CTGCAG | 1 cut(s) | 873 |
| PvuII | CAGCTG | 1 cut(s) | 62 |
| RsaI | GTAC | 6 cut(s) | 11, 70, 580, 605, 709, 859 |
| RsaNI | GTAC | 6 cut(s) | 10, 69, 579, 604, 708, 858 |
| RseI | CAYNNNNRTG | 1 cut(s) | 270 |
| SacII | CCGCGG | 1 cut(s) | 43 |
| SaqAI | TTAA | 5 cut(s) | 146, 167, 389, 876, 905 |
| SatI | GCNGC | 4 cut(s) | 43, 490, 610, 676 |
| Sau3AI | GATC | 5 cut(s) | 15, 262, 268, 457, 802 |
| Sau96I | GGNCC | 2 cut(s) | 24, 44 |
| SbfI | CCTGCAGG | 1 cut(s) | 873 |
| ScaI | AGTACT | 1 cut(s) | 859 |
| ScrFI | CCNGG | 2 cut(s) | 82, 399 |
| SdaI | CCTGCAGG | 1 cut(s) | 873 |
| SfaNI | GCATC | 1 cut(s) | 76 |
| SfcI | CTRYAG | 1 cut(s) | 869 |
| Sfr303I | CCGCGG | 1 cut(s) | 43 |
| SgrBI | CCGCGG | 1 cut(s) | 43 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 270 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 873 |
| Sse9I | AATT | 2 cut(s) | 417, 836 |
| SsiI | CCGC | 3 cut(s) | 40, 42, 676 |
| SspI | AATATT | 2 cut(s) | 376, 616 |
| SspMI | CTAG | 2 cut(s) | 357, 777 |
| StyD4I | CCNGG | 2 cut(s) | 80, 397 |
| TaaI | ACNGT | 2 cut(s) | 439, 760 |
| TaqI | TCGA | 6 cut(s) | 5, 113, 347, 558, 691, 723 |
| TaqII | GACCGA | 1 cut(s) | 831 |
| TasI | AATT | 2 cut(s) | 417, 836 |
| TatI | WGTACW | 3 cut(s) | 68, 578, 857 |
| TauI | GCSGC | 2 cut(s) | 45, 678 |
| TfiI | GAWTC | 4 cut(s) | 50, 117, 179, 572 |
| Tru1I | TTAA | 5 cut(s) | 146, 167, 389, 876, 905 |
| Tru9I | TTAA | 5 cut(s) | 146, 167, 389, 876, 905 |
| TscAI | CASTG | 1 cut(s) | 458 |
| TseFI | GTSAC | 2 cut(s) | 622, 754 |
| TseI | GCWGC | 2 cut(s) | 489, 609 |
| Tsp45I | GTSAC | 2 cut(s) | 622, 754 |
| TspDTI | ATGAA | 5 cut(s) | 288, 303, 657, 663, 772 |
| TspGWI | ACGGA | 2 cut(s) | 19, 121 |
| TspRI | CASTG | 1 cut(s) | 458 |
| XapI | RAATTY | 1 cut(s) | 836 |
| XceI | RCATGY | 1 cut(s) | 660 |
| XspI | CTAG | 2 cut(s) | 357, 777 |
| ZrmI | AGTACT | 1 cut(s) | 859 |