E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 426 |
| AciI | CCGC | 1 cut(s) | 282 |
| AclWI | GGATC | 2 cut(s) | 82, 95 |
| AcoI | YGGCCR | 2 cut(s) | 1057, 1085 |
| AcsI | RAATTY | 2 cut(s) | 730, 791 |
| AcuI | CTGAAG | 3 cut(s) | 255, 672, 697 |
| AfaI | GTAC | 3 cut(s) | 752, 823, 888 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 504, 1016, 1069 |
| AflII | CTTAAG | 1 cut(s) | 1064 |
| AgsI | TTSAA | 5 cut(s) | 407, 520, 551, 806, 854 |
| AjnI | CCWGG | 3 cut(s) | 134, 892, 1051 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 237, 261, 269, 293 |
| AluBI | AGCT | 6 cut(s) | 155, 455, 545, 692, 700, 922 |
| AluI | AGCT | 6 cut(s) | 155, 455, 545, 692, 700, 922 |
| Alw21I | GWGCWC | 1 cut(s) | 157 |
| Alw26I | GTCTC | 3 cut(s) | 297, 348, 688 |
| AlwI | GGATC | 2 cut(s) | 82, 95 |
| AoxI | GGCC | 5 cut(s) | 72, 92, 137, 1057, 1085 |
| ApeKI | GCWGC | 2 cut(s) | 468, 697 |
| ApoI | RAATTY | 2 cut(s) | 730, 791 |
| AspS9I | GGNCC | 3 cut(s) | 42, 73, 138 |
| AsuC2I | CCSGG | 1 cut(s) | 1018 |
| AsuHPI | GGTGA | 2 cut(s) | 632, 639 |
| AvaII | GGWCC | 1 cut(s) | 42 |
| AxyI | CCTNAGG | 1 cut(s) | 174 |
| BaeGI | GKGCMC | 1 cut(s) | 450 |
| BalI | TGGCCA | 1 cut(s) | 1059 |
| BamHI | GGATCC | 1 cut(s) | 87 |
| BanII | GRGCYC | 1 cut(s) | 157 |
| Bbv12I | GWGCWC | 1 cut(s) | 157 |
| BbvI | GCAGC | 2 cut(s) | 455, 709 |
| BccI | CCATC | 5 cut(s) | 191, 332, 542, 852, 1063 |
| BceAI | ACGGC | 1 cut(s) | 513 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 746, 780 |
| BciT130I | CCWGG | 3 cut(s) | 136, 894, 1053 |
| BciVI | GTATCC | 1 cut(s) | 1106 |
| BclI | TGATCA | 1 cut(s) | 313 |
| BcnI | CCSGG | 1 cut(s) | 1018 |
| BcoDI | GTCTC | 3 cut(s) | 297, 348, 688 |
| BfaI | CTAG | 3 cut(s) | 18, 860, 1011 |
| BfrI | CTTAAG | 1 cut(s) | 1064 |
| BfuI | GTATCC | 1 cut(s) | 1106 |
| BisI | GCNGC | 2 cut(s) | 469, 698 |
| BlpI | GCTNAGC | 1 cut(s) | 693 |
| BlsI | GCNGC | 2 cut(s) | 470, 699 |
| Bme1390I | CCNGG | 4 cut(s) | 136, 894, 1018, 1053 |
| Bme18I | GGWCC | 1 cut(s) | 42 |
| BmgT120I | GGNCC | 3 cut(s) | 42, 73, 138 |
| BmiI | GGNNCC | 2 cut(s) | 44, 89 |
| BmrFI | CCNGG | 4 cut(s) | 136, 894, 1018, 1053 |
| BmsI | GCATC | 3 cut(s) | 188, 605, 997 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 693 |
| BpuEI | CTTGAG | 1 cut(s) | 821 |
| BpuMI | CCSGG | 1 cut(s) | 1018 |
| BsaI | GGTCTC | 1 cut(s) | 348 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 504, 1016, 1069 |
| Bse1I | ACTGG | 2 cut(s) | 51, 783 |
| Bse21I | CCTNAGG | 1 cut(s) | 174 |
| Bse3DI | GCAATG | 1 cut(s) | 876 |
| BseBI | CCWGG | 3 cut(s) | 136, 894, 1053 |
| BseGI | GGATG | 3 cut(s) | 179, 199, 343 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 504, 1016, 1069 |
| BseMI | GCAATG | 1 cut(s) | 876 |
| BseMII | CTCAG | 3 cut(s) | 188, 684, 927 |
| BseNI | ACTGG | 2 cut(s) | 51, 783 |
| BseRI | GAGGAG | 1 cut(s) | 451 |
| BseSI | GKGCMC | 1 cut(s) | 450 |
| BseXI | GCAGC | 2 cut(s) | 455, 709 |
| BsgI | GTGCAG | 1 cut(s) | 454 |
| BshFI | GGCC | 5 cut(s) | 74, 94, 139, 1059, 1087 |
| BsiHKAI | GWGCWC | 1 cut(s) | 157 |
| BsiSI | CCGG | 2 cut(s) | 91, 1017 |
| BslFI | GGGAC | 2 cut(s) | 28, 477 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 504, 1016, 1069 |
| BsmAI | GTCTC | 3 cut(s) | 297, 348, 688 |
| BsmBI | CGTCTC | 1 cut(s) | 297 |
| BsmFI | GGGAC | 2 cut(s) | 28, 477 |
| BsnI | GGCC | 5 cut(s) | 74, 94, 139, 1059, 1087 |
| Bso31I | GGTCTC | 1 cut(s) | 348 |
| Bsp1286I | GDGCHC | 2 cut(s) | 157, 450 |
| Bsp1407I | TGTACA | 1 cut(s) | 886 |
| Bsp143I | GATC | 2 cut(s) | 87, 313 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 693 |
| BspACI | CCGC | 1 cut(s) | 282 |
| BspANI | GGCC | 5 cut(s) | 74, 94, 139, 1059, 1087 |
| BspCNI | CTCAG | 3 cut(s) | 187, 685, 928 |
| BspLI | GGNNCC | 2 cut(s) | 44, 89 |
| BspPI | GGATC | 2 cut(s) | 82, 95 |
| BspQI | GCTCTTC | 1 cut(s) | 324 |
| BspTI | CTTAAG | 1 cut(s) | 1064 |
| BspTNI | GGTCTC | 1 cut(s) | 348 |
| BsrDI | GCAATG | 1 cut(s) | 876 |
| BsrGI | TGTACA | 1 cut(s) | 886 |
| BsrI | ACTGG | 2 cut(s) | 51, 783 |
| BssMI | GATC | 2 cut(s) | 87, 313 |
| Bst2UI | CCWGG | 3 cut(s) | 136, 894, 1053 |
| Bst4CI | ACNGT | 1 cut(s) | 761 |
| Bst6I | CTCTTC | 4 cut(s) | 195, 210, 324, 691 |
| BstAFI | CTTAAG | 1 cut(s) | 1064 |
| BstAUI | TGTACA | 1 cut(s) | 886 |
| BstC8I | GCNNGC | 3 cut(s) | 96, 457, 986 |
| BstDEI | CTNAG | 3 cut(s) | 174, 693, 936 |
| BstF5I | GGATG | 3 cut(s) | 179, 199, 343 |
| BstKTI | GATC | 2 cut(s) | 90, 316 |
| BstMAI | GTCTC | 3 cut(s) | 297, 348, 688 |
| BstMBI | GATC | 2 cut(s) | 87, 313 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 399, 465, 602 |
| BstNI | CCWGG | 3 cut(s) | 136, 894, 1053 |
| BstNSI | RCATGY | 2 cut(s) | 15, 639 |
| BstSCI | CCNGG | 4 cut(s) | 134, 892, 1016, 1051 |
| BstSLI | GKGCMC | 1 cut(s) | 450 |
| BstV1I | GCAGC | 2 cut(s) | 455, 709 |
| BstX2I | RGATCY | 1 cut(s) | 87 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 190 |
| BstYI | RGATCY | 1 cut(s) | 87 |
| Bsu36I | CCTNAGG | 1 cut(s) | 174 |
| BsuI | GTATCC | 1 cut(s) | 1106 |
| BsuRI | GGCC | 5 cut(s) | 74, 94, 139, 1059, 1087 |
| BtsCI | GGATG | 3 cut(s) | 179, 199, 343 |
| BtsIMutI | CAGTG | 2 cut(s) | 58, 790 |
| Cac8I | GCNNGC | 3 cut(s) | 96, 457, 986 |
| Cfr13I | GGNCC | 3 cut(s) | 42, 73, 138 |
| Csp6I | GTAC | 3 cut(s) | 751, 822, 887 |
| CviAII | CATG | 7 cut(s) | 12, 70, 142, 353, 636, 725, 901 |
| CviQI | GTAC | 3 cut(s) | 751, 822, 887 |
| DdeI | CTNAG | 3 cut(s) | 174, 693, 936 |
| DpnI | GATC | 2 cut(s) | 89, 315 |
| DpnII | GATC | 2 cut(s) | 87, 313 |
| EaeI | YGGCCR | 2 cut(s) | 1057, 1085 |
| Eam1104I | CTCTTC | 4 cut(s) | 195, 210, 324, 691 |
| EarI | CTCTTC | 4 cut(s) | 195, 210, 324, 691 |
| EciI | GGCGGA | 1 cut(s) | 297 |
| Ecl136II | GAGCTC | 1 cut(s) | 155 |
| Eco24I | GRGCYC | 1 cut(s) | 157 |
| Eco31I | GGTCTC | 1 cut(s) | 348 |
| Eco47I | GGWCC | 1 cut(s) | 42 |
| Eco53kI | GAGCTC | 1 cut(s) | 155 |
| Eco57I | CTGAAG | 3 cut(s) | 255, 672, 697 |
| Eco81I | CCTNAGG | 1 cut(s) | 174 |
| EcoICRI | GAGCTC | 1 cut(s) | 155 |
| EcoO109I | RGGNCCY | 1 cut(s) | 42 |
| EcoRI | GAATTC | 1 cut(s) | 730 |
| EcoRII | CCWGG | 3 cut(s) | 134, 892, 1051 |
| EcoT22I | ATGCAT | 1 cut(s) | 147 |
| EcoT38I | GRGCYC | 1 cut(s) | 157 |
| Esp3I | CGTCTC | 1 cut(s) | 297 |
| FaeI | CATG | 7 cut(s) | 15, 73, 145, 356, 639, 728, 904 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1083, 1115 |
| FaqI | GGGAC | 2 cut(s) | 28, 477 |
| FatI | CATG | 7 cut(s) | 11, 69, 141, 352, 635, 724, 900 |
| FauNDI | CATATG | 2 cut(s) | 118, 1082 |
| FbaI | TGATCA | 1 cut(s) | 313 |
| FblI | GTMKAC | 1 cut(s) | 426 |
| Fnu4HI | GCNGC | 2 cut(s) | 469, 698 |
| FokI | GGATG | 3 cut(s) | 166, 206, 350 |
| FriOI | GRGCYC | 1 cut(s) | 157 |
| Fsp4HI | GCNGC | 2 cut(s) | 469, 698 |
| FspBI | CTAG | 3 cut(s) | 18, 860, 1011 |
| GluI | GCNGC | 2 cut(s) | 469, 698 |
| HaeIII | GGCC | 5 cut(s) | 74, 94, 139, 1059, 1087 |
| HapII | CCGG | 2 cut(s) | 91, 1017 |
| Hin1II | CATG | 7 cut(s) | 15, 73, 145, 356, 639, 728, 904 |
| HincII | GTYRAC | 1 cut(s) | 130 |
| HindII | GTYRAC | 1 cut(s) | 130 |
| HinfI | GANTC | 4 cut(s) | 113, 163, 516, 551 |
| HpaI | GTTAAC | 1 cut(s) | 130 |
| HpaII | CCGG | 2 cut(s) | 91, 1017 |
| HphI | GGTGA | 2 cut(s) | 632, 639 |
| Hpy166II | GTNNAC | 4 cut(s) | 130, 427, 623, 887 |
| Hpy188I | TCNGA | 1 cut(s) | 235 |
| Hpy188III | TCNNGA | 4 cut(s) | 538, 548, 806, 838 |
| Hpy8I | GTNNAC | 4 cut(s) | 130, 427, 623, 887 |
| HpyAV | CCTTC | 1 cut(s) | 1109 |
| HpyCH4III | ACNGT | 1 cut(s) | 761 |
| HpyCH4V | TGCA | 9 cut(s) | 98, 145, 471, 591, 675, 737, 869, 988, 1080 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 399, 465, 602 |
| HpyF3I | CTNAG | 3 cut(s) | 174, 693, 936 |
| Hsp92II | CATG | 7 cut(s) | 15, 73, 145, 356, 639, 728, 904 |
| Ksp22I | TGATCA | 1 cut(s) | 313 |
| KspAI | GTTAAC | 1 cut(s) | 130 |
| Kzo9I | GATC | 2 cut(s) | 87, 313 |
| LguI | GCTCTTC | 1 cut(s) | 324 |
| LmnI | GCTCC | 1 cut(s) | 464 |
| Lsp1109I | GCAGC | 2 cut(s) | 455, 709 |
| LweI | GCATC | 3 cut(s) | 188, 605, 997 |
| MaeI | CTAG | 3 cut(s) | 18, 860, 1011 |
| MaeIII | GTNAC | 2 cut(s) | 434, 785 |
| MalI | GATC | 2 cut(s) | 89, 315 |
| MboI | GATC | 2 cut(s) | 87, 313 |
| MflI | RGATCY | 1 cut(s) | 87 |
| MhlI | GDGCHC | 2 cut(s) | 157, 450 |
| MlsI | TGGCCA | 1 cut(s) | 1059 |
| MluCI | AATT | 6 cut(s) | 730, 743, 791, 876, 943, 1029 |
| MluNI | TGGCCA | 1 cut(s) | 1059 |
| MmeI | TCCRAC | 1 cut(s) | 493 |
| Mox20I | TGGCCA | 1 cut(s) | 1059 |
| Mph1103I | ATGCAT | 1 cut(s) | 147 |
| MscI | TGGCCA | 1 cut(s) | 1059 |
| MseI | TTAA | 5 cut(s) | 129, 188, 776, 1028, 1065 |
| MslI | CAYNNNNRTG | 3 cut(s) | 10, 188, 351 |
| Msp20I | TGGCCA | 1 cut(s) | 1059 |
| MspA1I | CMGCKG | 1 cut(s) | 692 |
| MspCI | CTTAAG | 1 cut(s) | 1064 |
| MspI | CCGG | 2 cut(s) | 91, 1017 |
| MspR9I | CCNGG | 4 cut(s) | 136, 894, 1018, 1053 |
| MvaI | CCWGG | 3 cut(s) | 136, 894, 1053 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 399, 465, 602 |
| NciI | CCSGG | 1 cut(s) | 1018 |
| NdeI | CATATG | 2 cut(s) | 118, 1082 |
| NdeII | GATC | 2 cut(s) | 87, 313 |
| NlaIII | CATG | 7 cut(s) | 15, 73, 145, 356, 639, 728, 904 |
| NlaIV | GGNNCC | 2 cut(s) | 44, 89 |
| NmuCI | GTSAC | 2 cut(s) | 434, 785 |
| NsiI | ATGCAT | 1 cut(s) | 147 |
| NspI | RCATGY | 2 cut(s) | 15, 639 |
| PciSI | GCTCTTC | 1 cut(s) | 324 |
| PfeI | GAWTC | 4 cut(s) | 113, 163, 516, 551 |
| PkrI | GCNGC | 2 cut(s) | 470, 699 |
| PpuMI | RGGWCCY | 1 cut(s) | 42 |
| Psp124BI | GAGCTC | 1 cut(s) | 157 |
| Psp5II | RGGWCCY | 1 cut(s) | 42 |
| Psp6I | CCWGG | 3 cut(s) | 134, 892, 1051 |
| PspGI | CCWGG | 3 cut(s) | 134, 892, 1051 |
| PspN4I | GGNNCC | 2 cut(s) | 44, 89 |
| PspPI | GGNCC | 3 cut(s) | 42, 73, 138 |
| PspPPI | RGGWCCY | 1 cut(s) | 42 |
| PsuI | RGATCY | 1 cut(s) | 87 |
| PvuII | CAGCTG | 1 cut(s) | 692 |
| RsaI | GTAC | 3 cut(s) | 752, 823, 888 |
| RsaNI | GTAC | 3 cut(s) | 751, 822, 887 |
| RseI | CAYNNNNRTG | 3 cut(s) | 10, 188, 351 |
| SacI | GAGCTC | 1 cut(s) | 157 |
| SapI | GCTCTTC | 1 cut(s) | 324 |
| SaqAI | TTAA | 5 cut(s) | 129, 188, 776, 1028, 1065 |
| SatI | GCNGC | 2 cut(s) | 469, 698 |
| Sau3AI | GATC | 2 cut(s) | 87, 313 |
| Sau96I | GGNCC | 3 cut(s) | 42, 73, 138 |
| ScrFI | CCNGG | 4 cut(s) | 136, 894, 1018, 1053 |
| SduI | GDGCHC | 2 cut(s) | 157, 450 |
| SfaNI | GCATC | 3 cut(s) | 188, 605, 997 |
| SinI | GGWCC | 1 cut(s) | 42 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 10, 188, 351 |
| SmlI | CTYRAG | 2 cut(s) | 836, 1064 |
| SmoI | CTYRAG | 2 cut(s) | 836, 1064 |
| Sse9I | AATT | 6 cut(s) | 730, 743, 791, 876, 943, 1029 |
| SsiI | CCGC | 1 cut(s) | 282 |
| SspMI | CTAG | 3 cut(s) | 18, 860, 1011 |
| SstI | GAGCTC | 1 cut(s) | 157 |
| StyD4I | CCNGG | 4 cut(s) | 134, 892, 1016, 1051 |
| TaaI | ACNGT | 1 cut(s) | 761 |
| TaqI | TCGA | 3 cut(s) | 157, 169, 537 |
| TasI | AATT | 6 cut(s) | 730, 743, 791, 876, 943, 1029 |
| TatI | WGTACW | 1 cut(s) | 886 |
| TfiI | GAWTC | 4 cut(s) | 113, 163, 516, 551 |
| Tru1I | TTAA | 5 cut(s) | 129, 188, 776, 1028, 1065 |
| Tru9I | TTAA | 5 cut(s) | 129, 188, 776, 1028, 1065 |
| TscAI | CASTG | 2 cut(s) | 58, 790 |
| TseFI | GTSAC | 2 cut(s) | 434, 785 |
| TseI | GCWGC | 2 cut(s) | 468, 697 |
| Tsp45I | GTSAC | 2 cut(s) | 434, 785 |
| TspDTI | ATGAA | 5 cut(s) | 105, 135, 569, 889, 986 |
| TspRI | CASTG | 2 cut(s) | 58, 790 |
| Vha464I | CTTAAG | 1 cut(s) | 1064 |
| VpaK11BI | GGWCC | 1 cut(s) | 42 |
| XapI | RAATTY | 2 cut(s) | 730, 791 |
| XceI | RCATGY | 2 cut(s) | 15, 639 |
| XmiI | GTMKAC | 1 cut(s) | 426 |
| XspI | CTAG | 3 cut(s) | 18, 860, 1011 |
| Zsp2I | ATGCAT | 1 cut(s) | 147 |