E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclI | AACGTT | 1 cut(s) | 522 |
| AcoI | YGGCCR | 2 cut(s) | 190, 349 |
| AcuI | CTGAAG | 1 cut(s) | 397 |
| AfaI | GTAC | 2 cut(s) | 460, 492 |
| AgsI | TTSAA | 1 cut(s) | 379 |
| AjnI | CCWGG | 1 cut(s) | 271 |
| AluBI | AGCT | 1 cut(s) | 417 |
| AluI | AGCT | 1 cut(s) | 417 |
| AoxI | GGCC | 3 cut(s) | 190, 349, 406 |
| AspS9I | GGNCC | 2 cut(s) | 44, 407 |
| AsuC2I | CCSGG | 1 cut(s) | 282 |
| AvaII | GGWCC | 1 cut(s) | 44 |
| BalI | TGGCCA | 1 cut(s) | 351 |
| BbsI | GAAGAC | 1 cut(s) | 27 |
| BccI | CCATC | 2 cut(s) | 245, 360 |
| BceAI | ACGGC | 1 cut(s) | 205 |
| BciT130I | CCWGG | 1 cut(s) | 273 |
| BcnI | CCSGG | 1 cut(s) | 282 |
| Bme1390I | CCNGG | 2 cut(s) | 273, 282 |
| Bme18I | GGWCC | 1 cut(s) | 44 |
| BmgT120I | GGNCC | 2 cut(s) | 44, 407 |
| BmiI | GGNNCC | 3 cut(s) | 46, 270, 409 |
| BmrFI | CCNGG | 2 cut(s) | 273, 282 |
| BmrI | ACTGGG | 1 cut(s) | 320 |
| BmuI | ACTGGG | 1 cut(s) | 320 |
| BpiI | GAAGAC | 1 cut(s) | 27 |
| BpuMI | CCSGG | 1 cut(s) | 282 |
| Bse1I | ACTGG | 2 cut(s) | 326, 334 |
| Bse3DI | GCAATG | 1 cut(s) | 19 |
| BseBI | CCWGG | 1 cut(s) | 273 |
| BseGI | GGATG | 1 cut(s) | 277 |
| BseMI | GCAATG | 1 cut(s) | 19 |
| BseNI | ACTGG | 2 cut(s) | 326, 334 |
| BseYI | CCCAGC | 1 cut(s) | 47 |
| BshFI | GGCC | 3 cut(s) | 192, 351, 408 |
| BsiSI | CCGG | 1 cut(s) | 282 |
| BslFI | GGGAC | 2 cut(s) | 308, 378 |
| BsmFI | GGGAC | 2 cut(s) | 308, 378 |
| BsnI | GGCC | 3 cut(s) | 192, 351, 408 |
| Bsp143I | GATC | 4 cut(s) | 106, 124, 196, 228 |
| BspANI | GGCC | 3 cut(s) | 192, 351, 408 |
| BspLI | GGNNCC | 3 cut(s) | 46, 270, 409 |
| BspQI | GCTCTTC | 1 cut(s) | 370 |
| BsrDI | GCAATG | 1 cut(s) | 19 |
| BsrI | ACTGG | 2 cut(s) | 326, 334 |
| BssMI | GATC | 4 cut(s) | 106, 124, 196, 228 |
| Bst2UI | CCWGG | 1 cut(s) | 273 |
| Bst4CI | ACNGT | 1 cut(s) | 322 |
| Bst6I | CTCTTC | 1 cut(s) | 370 |
| BstC8I | GCNNGC | 1 cut(s) | 53 |
| BstDEI | CTNAG | 2 cut(s) | 66, 420 |
| BstF5I | GGATG | 1 cut(s) | 277 |
| BstKTI | GATC | 4 cut(s) | 109, 127, 199, 231 |
| BstMBI | GATC | 4 cut(s) | 106, 124, 196, 228 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 303, 369, 414 |
| BstNI | CCWGG | 1 cut(s) | 273 |
| BstSCI | CCNGG | 2 cut(s) | 271, 280 |
| BstV2I | GAAGAC | 1 cut(s) | 27 |
| BsuRI | GGCC | 3 cut(s) | 192, 351, 408 |
| BtsCI | GGATG | 1 cut(s) | 277 |
| BtsIMutI | CAGTG | 1 cut(s) | 341 |
| Cac8I | GCNNGC | 1 cut(s) | 53 |
| Cfr13I | GGNCC | 2 cut(s) | 44, 407 |
| Csp6I | GTAC | 2 cut(s) | 459, 491 |
| CviAII | CATG | 1 cut(s) | 465 |
| CviJI | RGCY | 7 cut(s) | 51, 192, 205, 271, 351, 408, 417 |
| CviKI_1 | RGCY | 7 cut(s) | 51, 192, 205, 271, 351, 408, 417 |
| CviQI | GTAC | 2 cut(s) | 459, 491 |
| DdeI | CTNAG | 2 cut(s) | 66, 420 |
| DpnI | GATC | 4 cut(s) | 108, 126, 198, 230 |
| DpnII | GATC | 4 cut(s) | 106, 124, 196, 228 |
| EaeI | YGGCCR | 2 cut(s) | 190, 349 |
| Eam1104I | CTCTTC | 1 cut(s) | 370 |
| EarI | CTCTTC | 1 cut(s) | 370 |
| Eco47I | GGWCC | 1 cut(s) | 44 |
| Eco57I | CTGAAG | 1 cut(s) | 397 |
| EcoO109I | RGGNCCY | 1 cut(s) | 44 |
| EcoRII | CCWGG | 1 cut(s) | 271 |
| FaeI | CATG | 1 cut(s) | 468 |
| FaiI | YATR | 7 cut(s) | 183, 257, 309, 385, 387, 466, 504 |
| FaqI | GGGAC | 2 cut(s) | 308, 378 |
| FatI | CATG | 1 cut(s) | 464 |
| FokI | GGATG | 1 cut(s) | 264 |
| GsaI | CCCAGC | 1 cut(s) | 51 |
| HaeIII | GGCC | 3 cut(s) | 192, 351, 408 |
| HapII | CCGG | 1 cut(s) | 282 |
| Hin1II | CATG | 1 cut(s) | 468 |
| HinfI | GANTC | 3 cut(s) | 224, 452, 499 |
| HpaII | CCGG | 1 cut(s) | 282 |
| Hpy188I | TCNGA | 2 cut(s) | 61, 498 |
| Hpy188III | TCNNGA | 3 cut(s) | 80, 128, 200 |
| HpyAV | CCTTC | 2 cut(s) | 84, 421 |
| HpyCH4III | ACNGT | 1 cut(s) | 322 |
| HpyCH4IV | ACGT | 2 cut(s) | 493, 522 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 303, 369, 414 |
| HpyF3I | CTNAG | 2 cut(s) | 66, 420 |
| HpySE526I | ACGT | 2 cut(s) | 493, 522 |
| Hsp92II | CATG | 1 cut(s) | 468 |
| Kzo9I | GATC | 4 cut(s) | 106, 124, 196, 228 |
| LguI | GCTCTTC | 1 cut(s) | 370 |
| LmnI | GCTCC | 1 cut(s) | 268 |
| MaeII | ACGT | 2 cut(s) | 493, 522 |
| MaeIII | GTNAC | 1 cut(s) | 338 |
| MalI | GATC | 4 cut(s) | 108, 126, 198, 230 |
| MboI | GATC | 4 cut(s) | 106, 124, 196, 228 |
| MlsI | TGGCCA | 1 cut(s) | 351 |
| MluCI | AATT | 4 cut(s) | 300, 357, 374, 479 |
| MluNI | TGGCCA | 1 cut(s) | 351 |
| MlyI | GAGTC | 1 cut(s) | 508 |
| MnlI | CCTC | 1 cut(s) | 35 |
| Mox20I | TGGCCA | 1 cut(s) | 351 |
| MscI | TGGCCA | 1 cut(s) | 351 |
| MseI | TTAA | 3 cut(s) | 263, 317, 510 |
| Msp20I | TGGCCA | 1 cut(s) | 351 |
| MspI | CCGG | 1 cut(s) | 282 |
| MspR9I | CCNGG | 2 cut(s) | 273, 282 |
| MvaI | CCWGG | 1 cut(s) | 273 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 303, 369, 414 |
| NciI | CCSGG | 1 cut(s) | 282 |
| NdeII | GATC | 4 cut(s) | 106, 124, 196, 228 |
| NlaIII | CATG | 1 cut(s) | 468 |
| NlaIV | GGNNCC | 3 cut(s) | 46, 270, 409 |
| NmuCI | GTSAC | 1 cut(s) | 338 |
| PciSI | GCTCTTC | 1 cut(s) | 370 |
| PfeI | GAWTC | 2 cut(s) | 224, 452 |
| PfoI | TCCNGGA | 1 cut(s) | 280 |
| PleI | GAGTC | 1 cut(s) | 507 |
| PpsI | GAGTC | 1 cut(s) | 507 |
| PpuMI | RGGWCCY | 1 cut(s) | 44 |
| Psp1406I | AACGTT | 1 cut(s) | 522 |
| Psp5II | RGGWCCY | 1 cut(s) | 44 |
| Psp6I | CCWGG | 1 cut(s) | 271 |
| PspFI | CCCAGC | 1 cut(s) | 47 |
| PspGI | CCWGG | 1 cut(s) | 271 |
| PspN4I | GGNNCC | 3 cut(s) | 46, 270, 409 |
| PspPI | GGNCC | 2 cut(s) | 44, 407 |
| PspPPI | RGGWCCY | 1 cut(s) | 44 |
| RsaI | GTAC | 2 cut(s) | 460, 492 |
| RsaNI | GTAC | 2 cut(s) | 459, 491 |
| SapI | GCTCTTC | 1 cut(s) | 370 |
| SaqAI | TTAA | 3 cut(s) | 263, 317, 510 |
| Sau3AI | GATC | 4 cut(s) | 106, 124, 196, 228 |
| Sau96I | GGNCC | 2 cut(s) | 44, 407 |
| SchI | GAGTC | 1 cut(s) | 508 |
| ScrFI | CCNGG | 2 cut(s) | 273, 282 |
| SinI | GGWCC | 1 cut(s) | 44 |
| Sse9I | AATT | 4 cut(s) | 300, 357, 374, 479 |
| StyD4I | CCNGG | 2 cut(s) | 271, 280 |
| TaaI | ACNGT | 1 cut(s) | 322 |
| TaiI | ACGT | 2 cut(s) | 496, 525 |
| TaqI | TCGA | 1 cut(s) | 227 |
| TasI | AATT | 4 cut(s) | 300, 357, 374, 479 |
| TatI | WGTACW | 1 cut(s) | 458 |
| TfiI | GAWTC | 2 cut(s) | 224, 452 |
| Tru1I | TTAA | 3 cut(s) | 263, 317, 510 |
| Tru9I | TTAA | 3 cut(s) | 263, 317, 510 |
| TscAI | CASTG | 1 cut(s) | 341 |
| TseFI | GTSAC | 1 cut(s) | 338 |
| Tsp45I | GTSAC | 1 cut(s) | 338 |
| TspDTI | ATGAA | 3 cut(s) | 17, 45, 324 |
| TspRI | CASTG | 1 cut(s) | 341 |
| VpaK11BI | GGWCC | 1 cut(s) | 44 |