E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 2 cut(s) | 567, 1021 |
| AccII | CGCG | 1 cut(s) | 672 |
| AciI | CCGC | 2 cut(s) | 670, 1207 |
| AcoI | YGGCCR | 1 cut(s) | 1186 |
| AcsI | RAATTY | 1 cut(s) | 1000 |
| AcuI | CTGAAG | 2 cut(s) | 832, 1018 |
| AcyI | GRCGYC | 1 cut(s) | 131 |
| AfaI | GTAC | 4 cut(s) | 45, 462, 613, 887 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 676, 1035, 1145 |
| AgsI | TTSAA | 5 cut(s) | 242, 692, 883, 941, 989 |
| AjnI | CCWGG | 2 cut(s) | 56, 1180 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 13, 45 |
| AluBI | AGCT | 4 cut(s) | 596, 927, 1057, 1192 |
| AluI | AGCT | 4 cut(s) | 596, 927, 1057, 1192 |
| Alw21I | GWGCWC | 2 cut(s) | 79, 1112 |
| Alw26I | GTCTC | 3 cut(s) | 489, 823, 1086 |
| AoxI | GGCC | 4 cut(s) | 54, 83, 1095, 1186 |
| ApeKI | GCWGC | 2 cut(s) | 472, 855 |
| ApoI | RAATTY | 1 cut(s) | 1000 |
| AspLEI | GCGC | 1 cut(s) | 674 |
| AspS9I | GGNCC | 2 cut(s) | 54, 1096 |
| AsuC2I | CCSGG | 2 cut(s) | 646, 1029 |
| AsuHPI | GGTGA | 2 cut(s) | 773, 780 |
| BaeGI | GKGCMC | 1 cut(s) | 591 |
| BalI | TGGCCA | 1 cut(s) | 1188 |
| BanII | GRGCYC | 1 cut(s) | 552 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 444, 476 |
| BauI | CACGAG | 1 cut(s) | 150 |
| BbsI | GAAGAC | 1 cut(s) | 399 |
| Bbv12I | GWGCWC | 2 cut(s) | 79, 1112 |
| BbvI | GCAGC | 2 cut(s) | 484, 867 |
| BccI | CCATC | 3 cut(s) | 473, 566, 1192 |
| BceAI | ACGGC | 1 cut(s) | 594 |
| BciT130I | CCWGG | 2 cut(s) | 58, 1182 |
| BcnI | CCSGG | 2 cut(s) | 646, 1029 |
| BcoDI | GTCTC | 3 cut(s) | 489, 823, 1086 |
| BglII | AGATCT | 1 cut(s) | 68 |
| BisI | GCNGC | 3 cut(s) | 473, 670, 856 |
| BlsI | GCNGC | 3 cut(s) | 474, 671, 857 |
| Bme1390I | CCNGG | 4 cut(s) | 58, 646, 1029, 1182 |
| BmgT120I | GGNCC | 2 cut(s) | 54, 1096 |
| BmiI | GGNNCC | 1 cut(s) | 62 |
| BmrFI | CCNGG | 4 cut(s) | 58, 646, 1029, 1182 |
| BmsI | GCATC | 1 cut(s) | 719 |
| BpiI | GAAGAC | 1 cut(s) | 399 |
| BpmI | CTGGAG | 2 cut(s) | 47, 807 |
| BpuEI | CTTGAG | 2 cut(s) | 956, 1178 |
| BpuMI | CCSGG | 2 cut(s) | 646, 1029 |
| BsaAI | YACGTR | 1 cut(s) | 101 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1202 |
| BsaHI | GRCGYC | 1 cut(s) | 131 |
| BsaI | GGTCTC | 1 cut(s) | 489 |
| BsaJI | CCNNGG | 1 cut(s) | 134 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 676, 1035, 1145 |
| Bse118I | RCCGGY | 1 cut(s) | 1119 |
| Bse3DI | GCAATG | 2 cut(s) | 681, 805 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1202 |
| BseBI | CCWGG | 2 cut(s) | 58, 1182 |
| BseDI | CCNNGG | 1 cut(s) | 134 |
| BseGI | GGATG | 4 cut(s) | 40, 484, 558, 734 |
| BseJI | GATNNNNATC | 1 cut(s) | 1202 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 676, 1035, 1145 |
| BseMI | GCAATG | 2 cut(s) | 681, 805 |
| BseRI | GAGGAG | 5 cut(s) | 228, 264, 303, 342, 592 |
| BseSI | GKGCMC | 1 cut(s) | 591 |
| BseXI | GCAGC | 2 cut(s) | 484, 867 |
| Bsh1236I | CGCG | 1 cut(s) | 672 |
| BshFI | GGCC | 4 cut(s) | 56, 85, 1097, 1188 |
| BsiHKAI | GWGCWC | 2 cut(s) | 79, 1112 |
| BsiSI | CCGG | 3 cut(s) | 645, 1029, 1120 |
| BslFI | GGGAC | 1 cut(s) | 618 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 676, 1035, 1145 |
| BsmAI | GTCTC | 3 cut(s) | 489, 823, 1086 |
| BsmFI | GGGAC | 1 cut(s) | 618 |
| BsnI | GGCC | 4 cut(s) | 56, 85, 1097, 1188 |
| Bso31I | GGTCTC | 1 cut(s) | 489 |
| Bsp1286I | GDGCHC | 4 cut(s) | 79, 552, 591, 1112 |
| Bsp143I | GATC | 1 cut(s) | 68 |
| BspACI | CCGC | 2 cut(s) | 670, 1207 |
| BspANI | GGCC | 4 cut(s) | 56, 85, 1097, 1188 |
| BspFNI | CGCG | 1 cut(s) | 672 |
| BspLI | GGNNCC | 1 cut(s) | 62 |
| BspQI | GCTCTTC | 1 cut(s) | 432 |
| BspTNI | GGTCTC | 1 cut(s) | 489 |
| BsrDI | GCAATG | 2 cut(s) | 681, 805 |
| BsrFI | RCCGGY | 1 cut(s) | 1119 |
| BssAI | RCCGGY | 1 cut(s) | 1119 |
| BssECI | CCNNGG | 1 cut(s) | 134 |
| BssMI | GATC | 1 cut(s) | 68 |
| BssNAI | GTATAC | 1 cut(s) | 1022 |
| BssNI | GRCGYC | 1 cut(s) | 131 |
| BssSI | CACGAG | 1 cut(s) | 150 |
| BssT1I | CCWWGG | 1 cut(s) | 134 |
| Bst1107I | GTATAC | 1 cut(s) | 1022 |
| Bst2BI | CACGAG | 1 cut(s) | 150 |
| Bst2UI | CCWGG | 2 cut(s) | 58, 1182 |
| Bst4CI | ACNGT | 1 cut(s) | 893 |
| BstACI | GRCGYC | 1 cut(s) | 131 |
| BstBAI | YACGTR | 1 cut(s) | 101 |
| BstC8I | GCNNGC | 5 cut(s) | 598, 925, 1121, 1125, 1190 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1143 |
| BstF5I | GGATG | 4 cut(s) | 40, 484, 558, 734 |
| BstFNI | CGCG | 1 cut(s) | 672 |
| BstHHI | GCGC | 1 cut(s) | 674 |
| BstKTI | GATC | 1 cut(s) | 71 |
| BstMAI | GTCTC | 3 cut(s) | 489, 823, 1086 |
| BstMBI | GATC | 1 cut(s) | 68 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 540, 606, 807, 869, 1116 |
| BstNI | CCWGG | 2 cut(s) | 58, 1182 |
| BstNSI | RCATGY | 1 cut(s) | 780 |
| BstSCI | CCNGG | 4 cut(s) | 56, 644, 1027, 1180 |
| BstSLI | GKGCMC | 1 cut(s) | 591 |
| BstUI | CGCG | 1 cut(s) | 672 |
| BstV1I | GCAGC | 2 cut(s) | 484, 867 |
| BstV2I | GAAGAC | 1 cut(s) | 399 |
| BstX2I | RGATCY | 1 cut(s) | 68 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 71 |
| BstYI | RGATCY | 1 cut(s) | 68 |
| BstZ17I | GTATAC | 1 cut(s) | 1022 |
| BsuRI | GGCC | 4 cut(s) | 56, 85, 1097, 1188 |
| BtsCI | GGATG | 4 cut(s) | 40, 484, 558, 734 |
| BtsIMutI | CAGTG | 1 cut(s) | 1011 |
| Cac8I | GCNNGC | 5 cut(s) | 598, 925, 1121, 1125, 1190 |
| CfoI | GCGC | 1 cut(s) | 674 |
| Cfr10I | RCCGGY | 1 cut(s) | 1119 |
| Cfr13I | GGNCC | 2 cut(s) | 54, 1096 |
| CseI | GACGC | 1 cut(s) | 139 |
| Csp6I | GTAC | 4 cut(s) | 44, 461, 612, 886 |
| CviAII | CATG | 9 cut(s) | 93, 127, 426, 464, 494, 708, 777, 860, 1164 |
| CviQI | GTAC | 4 cut(s) | 44, 461, 612, 886 |
| DpnI | GATC | 1 cut(s) | 70 |
| DpnII | GATC | 1 cut(s) | 68 |
| EaeI | YGGCCR | 1 cut(s) | 1186 |
| Eco130I | CCWWGG | 1 cut(s) | 134 |
| Eco147I | AGGCCT | 1 cut(s) | 85 |
| Eco24I | GRGCYC | 1 cut(s) | 552 |
| Eco31I | GGTCTC | 1 cut(s) | 489 |
| Eco57I | CTGAAG | 2 cut(s) | 832, 1018 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1143 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1096 |
| EcoRII | CCWGG | 2 cut(s) | 56, 1180 |
| EcoT14I | CCWWGG | 1 cut(s) | 134 |
| EcoT38I | GRGCYC | 1 cut(s) | 552 |
| ErhI | CCWWGG | 1 cut(s) | 134 |
| FaeI | CATG | 9 cut(s) | 96, 130, 429, 467, 497, 711, 780, 863, 1167 |
| FaqI | GGGAC | 1 cut(s) | 618 |
| FatI | CATG | 9 cut(s) | 92, 126, 425, 463, 493, 707, 776, 859, 1163 |
| FblI | GTMKAC | 2 cut(s) | 567, 1021 |
| Fnu4HI | GCNGC | 3 cut(s) | 473, 670, 856 |
| FokI | GGATG | 4 cut(s) | 47, 491, 545, 741 |
| FriOI | GRGCYC | 1 cut(s) | 552 |
| Fsp4HI | GCNGC | 3 cut(s) | 473, 670, 856 |
| GlaI | GCGC | 1 cut(s) | 673 |
| GluI | GCNGC | 3 cut(s) | 473, 670, 856 |
| GsuI | CTGGAG | 2 cut(s) | 47, 807 |
| HaeIII | GGCC | 4 cut(s) | 56, 85, 1097, 1188 |
| HapII | CCGG | 3 cut(s) | 645, 1029, 1120 |
| HgaI | GACGC | 1 cut(s) | 139 |
| HhaI | GCGC | 1 cut(s) | 674 |
| Hin1I | GRCGYC | 1 cut(s) | 131 |
| Hin1II | CATG | 9 cut(s) | 96, 130, 429, 467, 497, 711, 780, 863, 1167 |
| Hin6I | GCGC | 1 cut(s) | 672 |
| HinP1I | GCGC | 1 cut(s) | 672 |
| HincII | GTYRAC | 1 cut(s) | 124 |
| HindII | GTYRAC | 1 cut(s) | 124 |
| HindIII | AAGCTT | 1 cut(s) | 925 |
| HinfI | GANTC | 4 cut(s) | 29, 107, 657, 692 |
| HpaI | GTTAAC | 1 cut(s) | 124 |
| HpaII | CCGG | 3 cut(s) | 645, 1029, 1120 |
| HphI | GGTGA | 2 cut(s) | 773, 780 |
| Hpy166II | GTNNAC | 4 cut(s) | 124, 568, 764, 1022 |
| Hpy188I | TCNGA | 4 cut(s) | 34, 112, 158, 851 |
| Hpy188III | TCNNGA | 6 cut(s) | 64, 689, 824, 941, 973, 1195 |
| Hpy8I | GTNNAC | 4 cut(s) | 124, 568, 764, 1022 |
| HpyAV | CCTTC | 3 cut(s) | 75, 413, 676 |
| HpyCH4III | ACNGT | 1 cut(s) | 893 |
| HpyCH4IV | ACGT | 2 cut(s) | 100, 160 |
| HpyCH4V | TGCA | 2 cut(s) | 732, 810 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 540, 606, 807, 869, 1116 |
| HpySE526I | ACGT | 2 cut(s) | 100, 160 |
| Hsp92I | GRCGYC | 1 cut(s) | 131 |
| Hsp92II | CATG | 9 cut(s) | 96, 130, 429, 467, 497, 711, 780, 863, 1167 |
| HspAI | GCGC | 1 cut(s) | 672 |
| KroI | GCCGGC | 1 cut(s) | 1119 |
| KroNI | GCCGGC | 1 cut(s) | 1121 |
| KspAI | GTTAAC | 1 cut(s) | 124 |
| Kzo9I | GATC | 1 cut(s) | 68 |
| LguI | GCTCTTC | 1 cut(s) | 432 |
| LmnI | GCTCC | 2 cut(s) | 66, 605 |
| Lsp1109I | GCAGC | 2 cut(s) | 484, 867 |
| LweI | GCATC | 1 cut(s) | 719 |
| MaeII | ACGT | 2 cut(s) | 100, 160 |
| MaeIII | GTNAC | 2 cut(s) | 96, 1012 |
| MalI | GATC | 1 cut(s) | 70 |
| MboI | GATC | 1 cut(s) | 68 |
| MfeI | CAATTG | 1 cut(s) | 1128 |
| MflI | RGATCY | 1 cut(s) | 68 |
| MhlI | GDGCHC | 4 cut(s) | 79, 552, 591, 1112 |
| MlsI | TGGCCA | 1 cut(s) | 1188 |
| MluCI | AATT | 7 cut(s) | 521, 878, 979, 1000, 1088, 1128, 1158 |
| MluNI | TGGCCA | 1 cut(s) | 1188 |
| MmeI | TCCRAC | 4 cut(s) | 205, 241, 280, 319 |
| Mox20I | TGGCCA | 1 cut(s) | 1188 |
| MroNI | GCCGGC | 1 cut(s) | 1119 |
| MscI | TGGCCA | 1 cut(s) | 1188 |
| MseI | TTAA | 6 cut(s) | 123, 408, 621, 911, 1157, 1231 |
| Msp20I | TGGCCA | 1 cut(s) | 1188 |
| MspI | CCGG | 3 cut(s) | 645, 1029, 1120 |
| MspR9I | CCNGG | 4 cut(s) | 58, 646, 1029, 1182 |
| MunI | CAATTG | 1 cut(s) | 1128 |
| MvaI | CCWGG | 2 cut(s) | 58, 1182 |
| MvnI | CGCG | 1 cut(s) | 672 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 540, 606, 807, 869, 1116 |
| NaeI | GCCGGC | 1 cut(s) | 1121 |
| NciI | CCSGG | 2 cut(s) | 646, 1029 |
| NdeII | GATC | 1 cut(s) | 68 |
| NgoMIV | GCCGGC | 1 cut(s) | 1119 |
| NlaIII | CATG | 9 cut(s) | 96, 130, 429, 467, 497, 711, 780, 863, 1167 |
| NlaIV | GGNNCC | 1 cut(s) | 62 |
| NspI | RCATGY | 1 cut(s) | 780 |
| PceI | AGGCCT | 1 cut(s) | 85 |
| PciSI | GCTCTTC | 1 cut(s) | 432 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 157 |
| PdiI | GCCGGC | 1 cut(s) | 1121 |
| PfeI | GAWTC | 4 cut(s) | 29, 107, 657, 692 |
| PkrI | GCNGC | 3 cut(s) | 474, 671, 857 |
| Ppu21I | YACGTR | 1 cut(s) | 101 |
| Psp6I | CCWGG | 2 cut(s) | 56, 1180 |
| PspGI | CCWGG | 2 cut(s) | 56, 1180 |
| PspN4I | GGNNCC | 1 cut(s) | 62 |
| PspPI | GGNCC | 2 cut(s) | 54, 1096 |
| PsuI | RGATCY | 1 cut(s) | 68 |
| RsaI | GTAC | 4 cut(s) | 45, 462, 613, 887 |
| RsaNI | GTAC | 4 cut(s) | 44, 461, 612, 886 |
| SapI | GCTCTTC | 1 cut(s) | 432 |
| SaqAI | TTAA | 6 cut(s) | 123, 408, 621, 911, 1157, 1231 |
| SatI | GCNGC | 3 cut(s) | 473, 670, 856 |
| Sau3AI | GATC | 1 cut(s) | 68 |
| Sau96I | GGNCC | 2 cut(s) | 54, 1096 |
| ScrFI | CCNGG | 4 cut(s) | 58, 646, 1029, 1182 |
| SduI | GDGCHC | 4 cut(s) | 79, 552, 591, 1112 |
| SfaNI | GCATC | 1 cut(s) | 719 |
| SmlI | CTYRAG | 2 cut(s) | 971, 1193 |
| SmoI | CTYRAG | 2 cut(s) | 971, 1193 |
| Sse9I | AATT | 7 cut(s) | 521, 878, 979, 1000, 1088, 1128, 1158 |
| SseBI | AGGCCT | 1 cut(s) | 85 |
| SsiI | CCGC | 2 cut(s) | 670, 1207 |
| StuI | AGGCCT | 1 cut(s) | 85 |
| StyD4I | CCNGG | 4 cut(s) | 56, 644, 1027, 1180 |
| StyI | CCWWGG | 1 cut(s) | 134 |
| TaaI | ACNGT | 1 cut(s) | 893 |
| TaiI | ACGT | 2 cut(s) | 103, 163 |
| TaqI | TCGA | 2 cut(s) | 79, 417 |
| TasI | AATT | 7 cut(s) | 521, 878, 979, 1000, 1088, 1128, 1158 |
| TatI | WGTACW | 2 cut(s) | 460, 885 |
| TauI | GCSGC | 1 cut(s) | 672 |
| TfiI | GAWTC | 4 cut(s) | 29, 107, 657, 692 |
| Tru1I | TTAA | 6 cut(s) | 123, 408, 621, 911, 1157, 1231 |
| Tru9I | TTAA | 6 cut(s) | 123, 408, 621, 911, 1157, 1231 |
| TscAI | CASTG | 1 cut(s) | 1011 |
| TseI | GCWGC | 2 cut(s) | 472, 855 |
| TspDTI | ATGAA | 9 cut(s) | 51, 81, 129, 219, 294, 333, 465, 480, 640 |
| TspGWI | ACGGA | 1 cut(s) | 194 |
| TspRI | CASTG | 1 cut(s) | 1011 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1143 |
| XapI | RAATTY | 1 cut(s) | 1000 |
| XceI | RCATGY | 1 cut(s) | 780 |
| XmiI | GTMKAC | 2 cut(s) | 567, 1021 |