E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 147, 526 |
| AccB1I | GGYRCC | 1 cut(s) | 761 |
| AciI | CCGC | 9 cut(s) | 704, 782, 807, 858, 958, 974, 993, 1098, 1101 |
| AclWI | GGATC | 6 cut(s) | 924, 940, 953, 1018, 1031, 1089 |
| AcoI | YGGCCR | 1 cut(s) | 678 |
| AcsI | RAATTY | 2 cut(s) | 346, 1066 |
| AcuI | CTGAAG | 1 cut(s) | 69 |
| AcyI | GRCGYC | 2 cut(s) | 674, 762 |
| AfaI | GTAC | 3 cut(s) | 149, 223, 911 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 28, 65, 437, 826, 1107 |
| AgsI | TTSAA | 7 cut(s) | 9, 112, 206, 254, 290, 320, 614 |
| AluBI | AGCT | 4 cut(s) | 89, 96, 738, 1012 |
| AluI | AGCT | 4 cut(s) | 89, 96, 738, 1012 |
| Alw21I | GWGCWC | 1 cut(s) | 313 |
| Alw26I | GTCTC | 1 cut(s) | 85 |
| AlwI | GGATC | 6 cut(s) | 924, 940, 953, 1018, 1031, 1089 |
| AoxI | GGCC | 5 cut(s) | 557, 587, 624, 678, 1109 |
| ApeKI | GCWGC | 2 cut(s) | 731, 877 |
| ApoI | RAATTY | 2 cut(s) | 346, 1066 |
| AspLEI | GCGC | 2 cut(s) | 764, 987 |
| AspS9I | GGNCC | 2 cut(s) | 558, 588 |
| AsuC2I | CCSGG | 2 cut(s) | 295, 943 |
| AsuHPI | GGTGA | 1 cut(s) | 1113 |
| BamHI | GGATCC | 2 cut(s) | 945, 1023 |
| BanI | GGYRCC | 1 cut(s) | 761 |
| BanII | GRGCYC | 1 cut(s) | 601 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 92, 124 |
| BbsI | GAAGAC | 2 cut(s) | 544, 682 |
| Bbv12I | GWGCWC | 1 cut(s) | 313 |
| BbvI | GCAGC | 2 cut(s) | 743, 889 |
| BccI | CCATC | 7 cut(s) | 705, 718, 793, 811, 890, 930, 957 |
| BcnI | CCSGG | 2 cut(s) | 295, 943 |
| BcoDI | GTCTC | 1 cut(s) | 85 |
| BfaI | CTAG | 6 cut(s) | 12, 90, 579, 585, 1053, 1080 |
| BfoI | RGCGCY | 1 cut(s) | 765 |
| BfuAI | ACCTGC | 2 cut(s) | 147, 526 |
| BglII | AGATCT | 1 cut(s) | 128 |
| BisI | GCNGC | 4 cut(s) | 732, 858, 878, 1101 |
| BlsI | GCNGC | 4 cut(s) | 733, 859, 879, 1102 |
| Bme1390I | CCNGG | 2 cut(s) | 295, 943 |
| BmgT120I | GGNCC | 2 cut(s) | 558, 588 |
| BmiI | GGNNCC | 3 cut(s) | 763, 947, 1025 |
| BmrFI | CCNGG | 2 cut(s) | 295, 943 |
| BmsI | GCATC | 2 cut(s) | 388, 536 |
| BpiI | GAAGAC | 2 cut(s) | 544, 682 |
| Bpu10I | CCTNAGC | 1 cut(s) | 621 |
| BpuEI | CTTGAG | 2 cut(s) | 338, 461 |
| BpuMI | CCSGG | 2 cut(s) | 295, 943 |
| BsaHI | GRCGYC | 2 cut(s) | 674, 762 |
| BsaJI | CCNNGG | 2 cut(s) | 293, 681 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 28, 65, 437, 826, 1107 |
| Bse118I | RCCGGY | 2 cut(s) | 676, 826 |
| Bse1I | ACTGG | 2 cut(s) | 653, 899 |
| Bse3DI | GCAATG | 1 cut(s) | 765 |
| BseDI | CCNNGG | 2 cut(s) | 293, 681 |
| BseGI | GGATG | 3 cut(s) | 183, 803, 922 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 28, 65, 437, 826, 1107 |
| BseMI | GCAATG | 1 cut(s) | 765 |
| BseNI | ACTGG | 2 cut(s) | 653, 899 |
| BseRI | GAGGAG | 1 cut(s) | 1066 |
| BseX3I | CGGCCG | 1 cut(s) | 678 |
| BseXI | GCAGC | 2 cut(s) | 743, 889 |
| BseYI | CCCAGC | 2 cut(s) | 1027, 1045 |
| BsgI | GTGCAG | 2 cut(s) | 279, 896 |
| Bsh1285I | CGRYCG | 1 cut(s) | 681 |
| BshFI | GGCC | 5 cut(s) | 559, 589, 626, 680, 1111 |
| BshNI | GGYRCC | 1 cut(s) | 761 |
| BsiEI | CGRYCG | 1 cut(s) | 681 |
| BsiHKAI | GWGCWC | 1 cut(s) | 313 |
| BsiSI | CCGG | 4 cut(s) | 295, 677, 827, 943 |
| BslFI | GGGAC | 1 cut(s) | 784 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 28, 65, 437, 826, 1107 |
| BsmAI | GTCTC | 1 cut(s) | 85 |
| BsmFI | GGGAC | 1 cut(s) | 784 |
| BsmI | GAATGC | 2 cut(s) | 40, 1031 |
| BsnI | GGCC | 5 cut(s) | 559, 589, 626, 680, 1111 |
| Bsp1286I | GDGCHC | 2 cut(s) | 313, 601 |
| Bsp1407I | TGTACA | 1 cut(s) | 147 |
| Bsp143I | GATC | 9 cut(s) | 128, 469, 480, 753, 852, 929, 945, 1023, 1094 |
| BspACI | CCGC | 9 cut(s) | 704, 782, 807, 858, 958, 974, 993, 1098, 1101 |
| BspANI | GGCC | 5 cut(s) | 559, 589, 626, 680, 1111 |
| BspHI | TCATGA | 1 cut(s) | 300 |
| BspLI | GGNNCC | 3 cut(s) | 763, 947, 1025 |
| BspMI | ACCTGC | 2 cut(s) | 147, 526 |
| BspPI | GGATC | 6 cut(s) | 924, 940, 953, 1018, 1031, 1089 |
| BspT107I | GGYRCC | 1 cut(s) | 761 |
| BsrDI | GCAATG | 1 cut(s) | 765 |
| BsrFI | RCCGGY | 2 cut(s) | 676, 826 |
| BsrGI | TGTACA | 1 cut(s) | 147 |
| BsrI | ACTGG | 2 cut(s) | 653, 899 |
| BssAI | RCCGGY | 2 cut(s) | 676, 826 |
| BssECI | CCNNGG | 2 cut(s) | 293, 681 |
| BssMI | GATC | 9 cut(s) | 128, 469, 480, 753, 852, 929, 945, 1023, 1094 |
| BssNI | GRCGYC | 2 cut(s) | 674, 762 |
| Bst4CI | ACNGT | 1 cut(s) | 874 |
| Bst6I | CTCTTC | 1 cut(s) | 88 |
| BstACI | GRCGYC | 2 cut(s) | 674, 762 |
| BstAUI | TGTACA | 1 cut(s) | 147 |
| BstC8I | GCNNGC | 8 cut(s) | 34, 38, 55, 678, 704, 736, 772, 983 |
| BstDEI | CTNAG | 2 cut(s) | 621, 1131 |
| BstF5I | GGATG | 3 cut(s) | 183, 803, 922 |
| BstH2I | RGCGCY | 1 cut(s) | 765 |
| BstHHI | GCGC | 2 cut(s) | 764, 987 |
| BstKTI | GATC | 9 cut(s) | 131, 472, 483, 756, 855, 932, 948, 1026, 1097 |
| BstMAI | GTCTC | 1 cut(s) | 85 |
| BstMBI | GATC | 9 cut(s) | 128, 469, 480, 753, 852, 929, 945, 1023, 1094 |
| BstMCI | CGRYCG | 1 cut(s) | 681 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 982 |
| BstNSI | RCATGY | 3 cut(s) | 36, 40, 620 |
| BstSCI | CCNGG | 2 cut(s) | 293, 941 |
| BstV1I | GCAGC | 2 cut(s) | 743, 889 |
| BstV2I | GAAGAC | 2 cut(s) | 544, 682 |
| BstX2I | RGATCY | 4 cut(s) | 128, 945, 1023, 1094 |
| BstYI | RGATCY | 4 cut(s) | 128, 945, 1023, 1094 |
| BstZI | CGGCCG | 1 cut(s) | 678 |
| BsuRI | GGCC | 5 cut(s) | 559, 589, 626, 680, 1111 |
| BtgZI | GCGATG | 1 cut(s) | 683 |
| BtsCI | GGATG | 3 cut(s) | 183, 803, 922 |
| BtsIMutI | CAGTG | 2 cut(s) | 870, 892 |
| BveI | ACCTGC | 2 cut(s) | 147, 526 |
| Cac8I | GCNNGC | 8 cut(s) | 34, 38, 55, 678, 704, 736, 772, 983 |
| CciI | TCATGA | 1 cut(s) | 300 |
| CfoI | GCGC | 2 cut(s) | 764, 987 |
| Cfr10I | RCCGGY | 2 cut(s) | 676, 826 |
| Cfr13I | GGNCC | 2 cut(s) | 558, 588 |
| CseI | GACGC | 3 cut(s) | 371, 682, 873 |
| Csp6I | GTAC | 3 cut(s) | 148, 222, 910 |
| CviQI | GTAC | 3 cut(s) | 148, 222, 910 |
| DdeI | CTNAG | 2 cut(s) | 621, 1131 |
| DinI | GGCGCC | 1 cut(s) | 763 |
| DpnI | GATC | 9 cut(s) | 130, 471, 482, 755, 854, 931, 947, 1025, 1096 |
| DpnII | GATC | 9 cut(s) | 128, 469, 480, 753, 852, 929, 945, 1023, 1094 |
| EaeI | YGGCCR | 1 cut(s) | 678 |
| EagI | CGGCCG | 1 cut(s) | 678 |
| Eam1104I | CTCTTC | 1 cut(s) | 88 |
| EarI | CTCTTC | 1 cut(s) | 88 |
| EciI | GGCGGA | 3 cut(s) | 796, 947, 1087 |
| EclXI | CGGCCG | 1 cut(s) | 678 |
| Eco24I | GRGCYC | 1 cut(s) | 601 |
| Eco52I | CGGCCG | 1 cut(s) | 678 |
| Eco57I | CTGAAG | 1 cut(s) | 69 |
| EcoT22I | ATGCAT | 2 cut(s) | 38, 42 |
| EcoT38I | GRGCYC | 1 cut(s) | 601 |
| EgeI | GGCGCC | 1 cut(s) | 763 |
| EheI | GGCGCC | 1 cut(s) | 763 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 400, 432, 487, 519 |
| FaqI | GGGAC | 1 cut(s) | 784 |
| FauI | CCCGC | 2 cut(s) | 711, 981 |
| Fnu4HI | GCNGC | 4 cut(s) | 732, 858, 878, 1101 |
| FokI | GGATG | 3 cut(s) | 170, 790, 909 |
| FriOI | GRGCYC | 1 cut(s) | 601 |
| Fsp4HI | GCNGC | 4 cut(s) | 732, 858, 878, 1101 |
| FspBI | CTAG | 6 cut(s) | 12, 90, 579, 585, 1053, 1080 |
| GlaI | GCGC | 2 cut(s) | 763, 986 |
| GluI | GCNGC | 4 cut(s) | 732, 858, 878, 1101 |
| GsaI | CCCAGC | 2 cut(s) | 1031, 1049 |
| HaeII | RGCGCY | 1 cut(s) | 765 |
| HaeIII | GGCC | 5 cut(s) | 559, 589, 626, 680, 1111 |
| HapII | CCGG | 4 cut(s) | 295, 677, 827, 943 |
| HgaI | GACGC | 3 cut(s) | 371, 682, 873 |
| HhaI | GCGC | 2 cut(s) | 764, 987 |
| Hin1I | GRCGYC | 2 cut(s) | 674, 762 |
| Hin6I | GCGC | 2 cut(s) | 762, 985 |
| HinP1I | GCGC | 2 cut(s) | 762, 985 |
| HinfI | GANTC | 4 cut(s) | 141, 241, 442, 461 |
| HpaII | CCGG | 4 cut(s) | 295, 677, 827, 943 |
| HphI | GGTGA | 1 cut(s) | 1113 |
| Hpy166II | GTNNAC | 2 cut(s) | 148, 658 |
| Hpy188I | TCNGA | 1 cut(s) | 1134 |
| Hpy188III | TCNNGA | 5 cut(s) | 112, 206, 301, 478, 933 |
| Hpy8I | GTNNAC | 2 cut(s) | 148, 658 |
| Hpy99I | CGWCG | 4 cut(s) | 647, 676, 721, 867 |
| HpyAV | CCTTC | 3 cut(s) | 212, 335, 1128 |
| HpyCH4III | ACNGT | 1 cut(s) | 874 |
| HpyCH4IV | ACGT | 1 cut(s) | 224 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 982 |
| HpyF3I | CTNAG | 2 cut(s) | 621, 1131 |
| HpySE526I | ACGT | 1 cut(s) | 224 |
| Hsp92I | GRCGYC | 2 cut(s) | 674, 762 |
| HspAI | GCGC | 2 cut(s) | 762, 985 |
| KasI | GGCGCC | 1 cut(s) | 761 |
| KroI | GCCGGC | 1 cut(s) | 676 |
| KroNI | GCCGGC | 1 cut(s) | 678 |
| Kzo9I | GATC | 9 cut(s) | 128, 469, 480, 753, 852, 929, 945, 1023, 1094 |
| LmnI | GCTCC | 4 cut(s) | 93, 316, 726, 872 |
| Lsp1109I | GCAGC | 2 cut(s) | 743, 889 |
| LweI | GCATC | 2 cut(s) | 388, 536 |
| MaeI | CTAG | 6 cut(s) | 12, 90, 579, 585, 1053, 1080 |
| MaeII | ACGT | 1 cut(s) | 224 |
| MaeIII | GTNAC | 2 cut(s) | 277, 937 |
| MalI | GATC | 9 cut(s) | 130, 471, 482, 755, 854, 931, 947, 1025, 1096 |
| MboI | GATC | 9 cut(s) | 128, 469, 480, 753, 852, 929, 945, 1023, 1094 |
| MboII | GAAGA | 7 cut(s) | 75, 194, 549, 682, 705, 859, 862 |
| MfeI | CAATTG | 3 cut(s) | 522, 609, 726 |
| MflI | RGATCY | 4 cut(s) | 128, 945, 1023, 1094 |
| MhlI | GDGCHC | 2 cut(s) | 313, 601 |
| MluCI | AATT | 6 cut(s) | 346, 522, 609, 726, 1066, 1086 |
| Mly113I | GGCGCC | 1 cut(s) | 762 |
| MmeI | TCCRAC | 4 cut(s) | 339, 455, 942, 1095 |
| Mph1103I | ATGCAT | 2 cut(s) | 38, 42 |
| MroNI | GCCGGC | 1 cut(s) | 676 |
| MseI | TTAA | 1 cut(s) | 393 |
| MspI | CCGG | 4 cut(s) | 295, 677, 827, 943 |
| MspR9I | CCNGG | 2 cut(s) | 295, 943 |
| MunI | CAATTG | 3 cut(s) | 522, 609, 726 |
| Mva1269I | GAATGC | 2 cut(s) | 40, 1031 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 982 |
| NaeI | GCCGGC | 1 cut(s) | 678 |
| NarI | GGCGCC | 1 cut(s) | 762 |
| NciI | CCSGG | 2 cut(s) | 295, 943 |
| NdeII | GATC | 9 cut(s) | 128, 469, 480, 753, 852, 929, 945, 1023, 1094 |
| NgoMIV | GCCGGC | 1 cut(s) | 676 |
| NlaIV | GGNNCC | 3 cut(s) | 763, 947, 1025 |
| NmeAIII | GCCGAG | 1 cut(s) | 706 |
| NmuCI | GTSAC | 1 cut(s) | 937 |
| NsiI | ATGCAT | 2 cut(s) | 38, 42 |
| NspI | RCATGY | 3 cut(s) | 36, 40, 620 |
| PaeI | GCATGC | 2 cut(s) | 36, 40 |
| PagI | TCATGA | 1 cut(s) | 300 |
| PctI | GAATGC | 2 cut(s) | 40, 1031 |
| PdiI | GCCGGC | 1 cut(s) | 678 |
| PfeI | GAWTC | 4 cut(s) | 141, 241, 442, 461 |
| PkrI | GCNGC | 4 cut(s) | 733, 859, 879, 1102 |
| PluTI | GGCGCC | 1 cut(s) | 765 |
| PspFI | CCCAGC | 2 cut(s) | 1027, 1045 |
| PspN4I | GGNNCC | 3 cut(s) | 763, 947, 1025 |
| PspPI | GGNCC | 2 cut(s) | 558, 588 |
| PsuI | RGATCY | 4 cut(s) | 128, 945, 1023, 1094 |
| RsaI | GTAC | 3 cut(s) | 149, 223, 911 |
| RsaNI | GTAC | 3 cut(s) | 148, 222, 910 |
| SaqAI | TTAA | 1 cut(s) | 393 |
| SatI | GCNGC | 4 cut(s) | 732, 858, 878, 1101 |
| Sau3AI | GATC | 9 cut(s) | 128, 469, 480, 753, 852, 929, 945, 1023, 1094 |
| Sau96I | GGNCC | 2 cut(s) | 558, 588 |
| ScrFI | CCNGG | 2 cut(s) | 295, 943 |
| SduI | GDGCHC | 2 cut(s) | 313, 601 |
| SfaNI | GCATC | 2 cut(s) | 388, 536 |
| SfoI | GGCGCC | 1 cut(s) | 763 |
| SmlI | CTYRAG | 2 cut(s) | 353, 476 |
| SmoI | CTYRAG | 2 cut(s) | 353, 476 |
| SphI | GCATGC | 2 cut(s) | 36, 40 |
| Sse9I | AATT | 6 cut(s) | 346, 522, 609, 726, 1066, 1086 |
| SsiI | CCGC | 9 cut(s) | 704, 782, 807, 858, 958, 974, 993, 1098, 1101 |
| SspDI | GGCGCC | 1 cut(s) | 761 |
| SspMI | CTAG | 6 cut(s) | 12, 90, 579, 585, 1053, 1080 |
| StyD4I | CCNGG | 2 cut(s) | 293, 941 |
| TaaI | ACNGT | 1 cut(s) | 874 |
| TaiI | ACGT | 1 cut(s) | 227 |
| TaqI | TCGA | 4 cut(s) | 483, 535, 885, 928 |
| TasI | AATT | 6 cut(s) | 346, 522, 609, 726, 1066, 1086 |
| TatI | WGTACW | 1 cut(s) | 147 |
| TauI | GCSGC | 2 cut(s) | 860, 1103 |
| TfiI | GAWTC | 4 cut(s) | 141, 241, 442, 461 |
| Tru1I | TTAA | 1 cut(s) | 393 |
| Tru9I | TTAA | 1 cut(s) | 393 |
| TscAI | CASTG | 2 cut(s) | 877, 899 |
| TseFI | GTSAC | 1 cut(s) | 937 |
| TseI | GCWGC | 2 cut(s) | 731, 877 |
| Tsp45I | GTSAC | 1 cut(s) | 937 |
| TspDTI | ATGAA | 6 cut(s) | 39, 231, 289, 317, 453, 852 |
| TspGWI | ACGGA | 3 cut(s) | 154, 653, 660 |
| TspRI | CASTG | 2 cut(s) | 877, 899 |
| XapI | RAATTY | 2 cut(s) | 346, 1066 |
| XceI | RCATGY | 3 cut(s) | 36, 40, 620 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 742 |
| XspI | CTAG | 6 cut(s) | 12, 90, 579, 585, 1053, 1080 |
| Zsp2I | ATGCAT | 2 cut(s) | 38, 42 |