E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 366 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 638 |
| AccII | CGCG | 1 cut(s) | 94 |
| AccIII | TCCGGA | 2 cut(s) | 5, 748 |
| AciI | CCGC | 4 cut(s) | 25, 75, 86, 566 |
| AclWI | GGATC | 2 cut(s) | 352, 441 |
| AcsI | RAATTY | 1 cut(s) | 813 |
| AcuI | CTGAAG | 1 cut(s) | 891 |
| AdeI | CACNNNGTG | 1 cut(s) | 626 |
| AfaI | GTAC | 2 cut(s) | 208, 665 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 367, 638, 680, 748 |
| AflIII | ACRYGT | 1 cut(s) | 40 |
| AgsI | TTSAA | 5 cut(s) | 59, 287, 542, 580, 805 |
| AjiI | CACGTC | 1 cut(s) | 156 |
| AjnI | CCWGG | 1 cut(s) | 631 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 362, 394 |
| AluBI | AGCT | 5 cut(s) | 14, 115, 191, 658, 851 |
| AluI | AGCT | 5 cut(s) | 14, 115, 191, 658, 851 |
| Alw21I | GWGCWC | 2 cut(s) | 631, 660 |
| Alw26I | GTCTC | 2 cut(s) | 162, 578 |
| AlwI | GGATC | 2 cut(s) | 352, 441 |
| Aor13HI | TCCGGA | 2 cut(s) | 5, 748 |
| ApoI | RAATTY | 1 cut(s) | 813 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 758 |
| AspLEI | GCGC | 2 cut(s) | 94, 239 |
| AspS9I | GGNCC | 2 cut(s) | 535, 554 |
| AsuHPI | GGTGA | 1 cut(s) | 800 |
| AvaII | GGWCC | 2 cut(s) | 535, 554 |
| BanII | GRGCYC | 1 cut(s) | 660 |
| Bbv12I | GWGCWC | 2 cut(s) | 631, 660 |
| BccI | CCATC | 2 cut(s) | 670, 802 |
| BceAI | ACGGC | 1 cut(s) | 12 |
| BciT130I | CCWGG | 1 cut(s) | 633 |
| BcoDI | GTCTC | 2 cut(s) | 162, 578 |
| BfaI | CTAG | 2 cut(s) | 116, 264 |
| BfmI | CTRYAG | 1 cut(s) | 439 |
| BfuAI | ACCTGC | 1 cut(s) | 366 |
| BglII | AGATCT | 1 cut(s) | 118 |
| BisI | GCNGC | 1 cut(s) | 25 |
| BlsI | GCNGC | 1 cut(s) | 26 |
| Bme1390I | CCNGG | 1 cut(s) | 633 |
| Bme18I | GGWCC | 2 cut(s) | 535, 554 |
| BmgBI | CACGTC | 1 cut(s) | 156 |
| BmgT120I | GGNCC | 2 cut(s) | 535, 554 |
| BmiI | GGNNCC | 2 cut(s) | 555, 556 |
| BmrFI | CCNGG | 1 cut(s) | 633 |
| BmsI | GCATC | 3 cut(s) | 73, 660, 913 |
| BpmI | CTGGAG | 1 cut(s) | 615 |
| BsaAI | YACGTR | 1 cut(s) | 515 |
| BsaWI | WCCGGW | 2 cut(s) | 5, 748 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 367, 638, 680, 748 |
| Bse1I | ACTGG | 2 cut(s) | 51, 169 |
| BseAI | TCCGGA | 2 cut(s) | 5, 748 |
| BseBI | CCWGG | 1 cut(s) | 633 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 367, 638, 680, 748 |
| BseMII | CTCAG | 2 cut(s) | 206, 321 |
| BseNI | ACTGG | 2 cut(s) | 51, 169 |
| BseRI | GAGGAG | 1 cut(s) | 725 |
| BsgI | GTGCAG | 1 cut(s) | 384 |
| Bsh1236I | CGCG | 1 cut(s) | 94 |
| BsiHKAI | GWGCWC | 2 cut(s) | 631, 660 |
| BsiSI | CCGG | 2 cut(s) | 6, 749 |
| BslFI | GGGAC | 3 cut(s) | 115, 117, 540 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 367, 638, 680, 748 |
| BsmAI | GTCTC | 2 cut(s) | 162, 578 |
| BsmBI | CGTCTC | 1 cut(s) | 162 |
| BsmFI | GGGAC | 3 cut(s) | 115, 117, 540 |
| Bsp1286I | GDGCHC | 2 cut(s) | 631, 660 |
| Bsp13I | TCCGGA | 2 cut(s) | 5, 748 |
| Bsp143I | GATC | 5 cut(s) | 95, 118, 260, 357, 433 |
| BspACI | CCGC | 4 cut(s) | 25, 75, 86, 566 |
| BspCNI | CTCAG | 2 cut(s) | 207, 322 |
| BspEI | TCCGGA | 2 cut(s) | 5, 748 |
| BspFNI | CGCG | 1 cut(s) | 94 |
| BspLI | GGNNCC | 2 cut(s) | 555, 556 |
| BspMI | ACCTGC | 1 cut(s) | 366 |
| BspPI | GGATC | 2 cut(s) | 352, 441 |
| BsrI | ACTGG | 2 cut(s) | 51, 169 |
| BssMI | GATC | 5 cut(s) | 95, 118, 260, 357, 433 |
| Bst2UI | CCWGG | 1 cut(s) | 633 |
| Bst4CI | ACNGT | 4 cut(s) | 395, 443, 607, 614 |
| Bst6I | CTCTTC | 1 cut(s) | 930 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 626 |
| BstBAI | YACGTR | 1 cut(s) | 515 |
| BstDEI | CTNAG | 2 cut(s) | 215, 330 |
| BstFNI | CGCG | 1 cut(s) | 94 |
| BstHHI | GCGC | 2 cut(s) | 94, 239 |
| BstKTI | GATC | 5 cut(s) | 98, 121, 263, 360, 436 |
| BstMAI | GTCTC | 2 cut(s) | 162, 578 |
| BstMBI | GATC | 5 cut(s) | 95, 118, 260, 357, 433 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 234, 626 |
| BstNI | CCWGG | 1 cut(s) | 633 |
| BstNSI | RCATGY | 1 cut(s) | 306 |
| BstSCI | CCNGG | 1 cut(s) | 631 |
| BstSFI | CTRYAG | 1 cut(s) | 439 |
| BstSNI | TACGTA | 1 cut(s) | 515 |
| BstUI | CGCG | 1 cut(s) | 94 |
| BstX2I | RGATCY | 2 cut(s) | 118, 433 |
| BstYI | RGATCY | 2 cut(s) | 118, 433 |
| BtgZI | GCGATG | 1 cut(s) | 96 |
| BtrI | CACGTC | 1 cut(s) | 156 |
| BtsIMutI | CAGTG | 5 cut(s) | 58, 176, 327, 526, 578 |
| BveI | ACCTGC | 1 cut(s) | 366 |
| CfoI | GCGC | 2 cut(s) | 94, 239 |
| Cfr13I | GGNCC | 2 cut(s) | 535, 554 |
| Csp6I | GTAC | 2 cut(s) | 207, 664 |
| CviAII | CATG | 7 cut(s) | 295, 303, 320, 362, 471, 507, 763 |
| CviJI | RGCY | 7 cut(s) | 14, 24, 115, 191, 511, 658, 851 |
| CviKI_1 | RGCY | 7 cut(s) | 14, 24, 115, 191, 511, 658, 851 |
| CviQI | GTAC | 2 cut(s) | 207, 664 |
| DdeI | CTNAG | 2 cut(s) | 215, 330 |
| DpnI | GATC | 5 cut(s) | 97, 120, 262, 359, 435 |
| DpnII | GATC | 5 cut(s) | 95, 118, 260, 357, 433 |
| DraIII | CACNNNGTG | 1 cut(s) | 626 |
| Eam1104I | CTCTTC | 1 cut(s) | 930 |
| EarI | CTCTTC | 1 cut(s) | 930 |
| EciI | GGCGGA | 1 cut(s) | 90 |
| Ecl136II | GAGCTC | 1 cut(s) | 658 |
| Eco105I | TACGTA | 1 cut(s) | 515 |
| Eco24I | GRGCYC | 1 cut(s) | 660 |
| Eco47I | GGWCC | 2 cut(s) | 535, 554 |
| Eco53kI | GAGCTC | 1 cut(s) | 658 |
| Eco57I | CTGAAG | 1 cut(s) | 891 |
| EcoICRI | GAGCTC | 1 cut(s) | 658 |
| EcoO109I | RGGNCCY | 1 cut(s) | 554 |
| EcoRII | CCWGG | 1 cut(s) | 631 |
| EcoT38I | GRGCYC | 1 cut(s) | 660 |
| Esp3I | CGTCTC | 1 cut(s) | 162 |
| FaeI | CATG | 7 cut(s) | 298, 306, 323, 365, 474, 510, 766 |
| FaqI | GGGAC | 3 cut(s) | 115, 117, 540 |
| FatI | CATG | 7 cut(s) | 294, 302, 319, 361, 470, 506, 762 |
| Fnu4HI | GCNGC | 1 cut(s) | 25 |
| FriOI | GRGCYC | 1 cut(s) | 660 |
| Fsp4HI | GCNGC | 1 cut(s) | 25 |
| FspBI | CTAG | 2 cut(s) | 116, 264 |
| GlaI | GCGC | 2 cut(s) | 93, 238 |
| GluI | GCNGC | 1 cut(s) | 25 |
| GsuI | CTGGAG | 1 cut(s) | 615 |
| HapII | CCGG | 2 cut(s) | 6, 749 |
| HhaI | GCGC | 2 cut(s) | 94, 239 |
| Hin1II | CATG | 7 cut(s) | 298, 306, 323, 365, 474, 510, 766 |
| Hin6I | GCGC | 2 cut(s) | 92, 237 |
| HinP1I | GCGC | 2 cut(s) | 92, 237 |
| HincII | GTYRAC | 1 cut(s) | 457 |
| HindII | GTYRAC | 1 cut(s) | 457 |
| HindIII | AAGCTT | 1 cut(s) | 12 |
| HinfI | GANTC | 4 cut(s) | 59, 475, 724, 866 |
| HpaI | GTTAAC | 1 cut(s) | 457 |
| HpaII | CCGG | 2 cut(s) | 6, 749 |
| HphI | GGTGA | 1 cut(s) | 800 |
| Hpy166II | GTNNAC | 2 cut(s) | 457, 610 |
| Hpy188I | TCNGA | 3 cut(s) | 709, 729, 871 |
| Hpy188III | TCNNGA | 2 cut(s) | 6, 749 |
| Hpy8I | GTNNAC | 2 cut(s) | 457, 610 |
| HpyAV | CCTTC | 5 cut(s) | 57, 240, 361, 548, 748 |
| HpyCH4III | ACNGT | 4 cut(s) | 395, 443, 607, 614 |
| HpyCH4IV | ACGT | 4 cut(s) | 40, 155, 453, 514 |
| HpyCH4V | TGCA | 6 cut(s) | 327, 375, 401, 411, 506, 819 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 234, 626 |
| HpyF3I | CTNAG | 2 cut(s) | 215, 330 |
| HpySE526I | ACGT | 4 cut(s) | 40, 155, 453, 514 |
| Hsp92II | CATG | 7 cut(s) | 298, 306, 323, 365, 474, 510, 766 |
| HspAI | GCGC | 2 cut(s) | 92, 237 |
| KflI | GGGWCCC | 1 cut(s) | 554 |
| Kpn2I | TCCGGA | 2 cut(s) | 5, 748 |
| KspAI | GTTAAC | 1 cut(s) | 457 |
| Kzo9I | GATC | 5 cut(s) | 95, 118, 260, 357, 433 |
| LmnI | GCTCC | 1 cut(s) | 634 |
| LweI | GCATC | 3 cut(s) | 73, 660, 913 |
| MaeI | CTAG | 2 cut(s) | 116, 264 |
| MaeII | ACGT | 4 cut(s) | 40, 155, 453, 514 |
| MaeIII | GTNAC | 7 cut(s) | 143, 173, 251, 298, 443, 601, 683 |
| MalI | GATC | 5 cut(s) | 97, 120, 262, 359, 435 |
| MboI | GATC | 5 cut(s) | 95, 118, 260, 357, 433 |
| MflI | RGATCY | 2 cut(s) | 118, 433 |
| MhlI | GDGCHC | 2 cut(s) | 631, 660 |
| MluCI | AATT | 2 cut(s) | 493, 813 |
| MnlI | CCTC | 5 cut(s) | 27, 71, 210, 674, 703 |
| MroI | TCCGGA | 2 cut(s) | 5, 748 |
| MroXI | GAANNNNTTC | 1 cut(s) | 758 |
| MseI | TTAA | 3 cut(s) | 456, 702, 830 |
| MslI | CAYNNNNRTG | 1 cut(s) | 695 |
| MspI | CCGG | 2 cut(s) | 6, 749 |
| MspR9I | CCNGG | 1 cut(s) | 633 |
| MvaI | CCWGG | 1 cut(s) | 633 |
| MvnI | CGCG | 1 cut(s) | 94 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 234, 626 |
| NdeII | GATC | 5 cut(s) | 95, 118, 260, 357, 433 |
| NlaIII | CATG | 7 cut(s) | 298, 306, 323, 365, 474, 510, 766 |
| NlaIV | GGNNCC | 2 cut(s) | 555, 556 |
| NmuCI | GTSAC | 5 cut(s) | 143, 173, 298, 443, 601 |
| NspI | RCATGY | 1 cut(s) | 306 |
| PdmI | GAANNNNTTC | 1 cut(s) | 758 |
| PfeI | GAWTC | 4 cut(s) | 59, 475, 724, 866 |
| PflFI | GACNNNGTC | 1 cut(s) | 552 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 638 |
| PfoI | TCCNGGA | 1 cut(s) | 631 |
| PkrI | GCNGC | 1 cut(s) | 26 |
| Ppu21I | YACGTR | 1 cut(s) | 515 |
| PpuMI | RGGWCCY | 1 cut(s) | 554 |
| Psp124BI | GAGCTC | 1 cut(s) | 660 |
| Psp5II | RGGWCCY | 1 cut(s) | 554 |
| Psp6I | CCWGG | 1 cut(s) | 631 |
| PspGI | CCWGG | 1 cut(s) | 631 |
| PspN4I | GGNNCC | 2 cut(s) | 555, 556 |
| PspPI | GGNCC | 2 cut(s) | 535, 554 |
| PspPPI | RGGWCCY | 1 cut(s) | 554 |
| PsuI | RGATCY | 2 cut(s) | 118, 433 |
| PsyI | GACNNNGTC | 1 cut(s) | 552 |
| RsaI | GTAC | 2 cut(s) | 208, 665 |
| RsaNI | GTAC | 2 cut(s) | 207, 664 |
| RseI | CAYNNNNRTG | 1 cut(s) | 695 |
| SacI | GAGCTC | 1 cut(s) | 660 |
| SaqAI | TTAA | 3 cut(s) | 456, 702, 830 |
| SatI | GCNGC | 1 cut(s) | 25 |
| Sau3AI | GATC | 5 cut(s) | 95, 118, 260, 357, 433 |
| Sau96I | GGNCC | 2 cut(s) | 535, 554 |
| ScrFI | CCNGG | 1 cut(s) | 633 |
| SduI | GDGCHC | 2 cut(s) | 631, 660 |
| SfaNI | GCATC | 3 cut(s) | 73, 660, 913 |
| SfcI | CTRYAG | 1 cut(s) | 439 |
| SinI | GGWCC | 2 cut(s) | 535, 554 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 695 |
| SnaBI | TACGTA | 1 cut(s) | 515 |
| Sse9I | AATT | 2 cut(s) | 493, 813 |
| SsiI | CCGC | 4 cut(s) | 25, 75, 86, 566 |
| SspMI | CTAG | 2 cut(s) | 116, 264 |
| SstI | GAGCTC | 1 cut(s) | 660 |
| StyD4I | CCNGG | 1 cut(s) | 631 |
| TaaI | ACNGT | 4 cut(s) | 395, 443, 607, 614 |
| TaiI | ACGT | 4 cut(s) | 43, 158, 456, 517 |
| TaqI | TCGA | 4 cut(s) | 17, 111, 126, 259 |
| TasI | AATT | 2 cut(s) | 493, 813 |
| TatI | WGTACW | 1 cut(s) | 206 |
| TauI | GCSGC | 1 cut(s) | 27 |
| TfiI | GAWTC | 4 cut(s) | 59, 475, 724, 866 |
| Tru1I | TTAA | 3 cut(s) | 456, 702, 830 |
| Tru9I | TTAA | 3 cut(s) | 456, 702, 830 |
| TscAI | CASTG | 5 cut(s) | 58, 176, 334, 526, 578 |
| TseFI | GTSAC | 5 cut(s) | 143, 173, 298, 443, 601 |
| Tsp45I | GTSAC | 5 cut(s) | 143, 173, 298, 443, 601 |
| TspDTI | ATGAA | 6 cut(s) | 731, 751, 855, 870, 876, 927 |
| TspGWI | ACGGA | 1 cut(s) | 52 |
| TspRI | CASTG | 5 cut(s) | 58, 176, 334, 526, 578 |
| Tth111I | GACNNNGTC | 1 cut(s) | 552 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 638 |
| VpaK11BI | GGWCC | 2 cut(s) | 535, 554 |
| XapI | RAATTY | 1 cut(s) | 813 |
| XceI | RCATGY | 1 cut(s) | 306 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 176 |
| XmnI | GAANNNNTTC | 1 cut(s) | 758 |
| XspI | CTAG | 2 cut(s) | 116, 264 |