E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 486, 983 |
| AatII | GACGTC | 1 cut(s) | 70 |
| AccI | GTMKAC | 1 cut(s) | 910 |
| AciI | CCGC | 3 cut(s) | 21, 273, 309 |
| AcoI | YGGCCR | 2 cut(s) | 339, 1012 |
| AcsI | RAATTY | 1 cut(s) | 528 |
| AcuI | CTGAAG | 1 cut(s) | 189 |
| AcyI | GRCGYC | 2 cut(s) | 67, 115 |
| AfaI | GTAC | 1 cut(s) | 719 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 318, 471, 635 |
| AflII | CTTAAG | 1 cut(s) | 59 |
| AgsI | TTSAA | 5 cut(s) | 657, 697, 735, 773, 854 |
| AjnI | CCWGG | 3 cut(s) | 53, 470, 988 |
| AluBI | AGCT | 5 cut(s) | 241, 278, 422, 729, 1018 |
| AluI | AGCT | 5 cut(s) | 241, 278, 422, 729, 1018 |
| Alw21I | GWGCWC | 2 cut(s) | 231, 357 |
| Alw26I | GTCTC | 3 cut(s) | 58, 655, 832 |
| AoxI | GGCC | 3 cut(s) | 339, 473, 1012 |
| ApeKI | GCWGC | 7 cut(s) | 48, 89, 275, 435, 639, 726, 957 |
| ApoI | RAATTY | 1 cut(s) | 528 |
| AspS9I | GGNCC | 5 cut(s) | 41, 109, 246, 321, 474 |
| AsuC2I | CCSGG | 1 cut(s) | 347 |
| AsuHPI | GGTGA | 4 cut(s) | 35, 230, 606, 847 |
| AvaII | GGWCC | 4 cut(s) | 41, 109, 246, 321 |
| BalI | TGGCCA | 1 cut(s) | 1014 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 653, 685 |
| BbsI | GAAGAC | 1 cut(s) | 388 |
| Bbv12I | GWGCWC | 2 cut(s) | 231, 357 |
| BbvI | GCAGC | 7 cut(s) | 60, 76, 287, 447, 626, 738, 969 |
| BccI | CCATC | 3 cut(s) | 26, 104, 779 |
| BceAI | ACGGC | 1 cut(s) | 102 |
| BciT130I | CCWGG | 3 cut(s) | 55, 472, 990 |
| BclI | TGATCA | 1 cut(s) | 220 |
| BcnI | CCSGG | 1 cut(s) | 347 |
| BcoDI | GTCTC | 3 cut(s) | 58, 655, 832 |
| BfaI | CTAG | 1 cut(s) | 858 |
| BfmI | CTRYAG | 3 cut(s) | 131, 433, 724 |
| BfrI | CTTAAG | 1 cut(s) | 59 |
| BisI | GCNGC | 7 cut(s) | 49, 90, 276, 436, 640, 727, 958 |
| BlsI | GCNGC | 7 cut(s) | 50, 91, 277, 437, 641, 728, 959 |
| Bme1390I | CCNGG | 4 cut(s) | 55, 347, 472, 990 |
| Bme18I | GGWCC | 4 cut(s) | 41, 109, 246, 321 |
| BmgT120I | GGNCC | 5 cut(s) | 41, 109, 246, 321, 474 |
| BmiI | GGNNCC | 2 cut(s) | 110, 976 |
| BmrFI | CCNGG | 4 cut(s) | 55, 347, 472, 990 |
| BpiI | GAAGAC | 1 cut(s) | 388 |
| BpuEI | CTTGAG | 1 cut(s) | 1004 |
| BpuMI | CCSGG | 1 cut(s) | 347 |
| BsaBI | GATNNNNATC | 1 cut(s) | 922 |
| BsaHI | GRCGYC | 2 cut(s) | 67, 115 |
| BsaJI | CCNNGG | 5 cut(s) | 53, 118, 312, 336, 988 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 318, 471, 635 |
| Bse1I | ACTGG | 1 cut(s) | 400 |
| Bse8I | GATNNNNATC | 1 cut(s) | 922 |
| BseBI | CCWGG | 3 cut(s) | 55, 472, 990 |
| BseDI | CCNNGG | 5 cut(s) | 53, 118, 312, 336, 988 |
| BseGI | GGATG | 4 cut(s) | 187, 355, 573, 911 |
| BseJI | GATNNNNATC | 1 cut(s) | 922 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 318, 471, 635 |
| BseMII | CTCAG | 1 cut(s) | 928 |
| BseNI | ACTGG | 1 cut(s) | 400 |
| BseRI | GAGGAG | 4 cut(s) | 51, 135, 138, 418 |
| BseXI | GCAGC | 7 cut(s) | 60, 76, 287, 447, 626, 738, 969 |
| BshFI | GGCC | 3 cut(s) | 341, 475, 1014 |
| BsiHKAI | GWGCWC | 2 cut(s) | 231, 357 |
| BsiSI | CCGG | 1 cut(s) | 347 |
| BslFI | GGGAC | 3 cut(s) | 122, 374, 444 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 318, 471, 635 |
| BsmAI | GTCTC | 3 cut(s) | 58, 655, 832 |
| BsmBI | CGTCTC | 1 cut(s) | 58 |
| BsmFI | GGGAC | 3 cut(s) | 122, 374, 444 |
| BsmI | GAATGC | 1 cut(s) | 610 |
| BsnI | GGCC | 3 cut(s) | 341, 475, 1014 |
| Bsp1286I | GDGCHC | 2 cut(s) | 231, 357 |
| Bsp1407I | TGTACA | 1 cut(s) | 717 |
| Bsp143I | GATC | 2 cut(s) | 220, 883 |
| BspACI | CCGC | 3 cut(s) | 21, 273, 309 |
| BspANI | GGCC | 3 cut(s) | 341, 475, 1014 |
| BspCNI | CTCAG | 1 cut(s) | 927 |
| BspLI | GGNNCC | 2 cut(s) | 110, 976 |
| BspMAI | CTGCAG | 2 cut(s) | 437, 728 |
| BspTI | CTTAAG | 1 cut(s) | 59 |
| BsrGI | TGTACA | 1 cut(s) | 717 |
| BsrI | ACTGG | 1 cut(s) | 400 |
| BssECI | CCNNGG | 5 cut(s) | 53, 118, 312, 336, 988 |
| BssMI | GATC | 2 cut(s) | 220, 883 |
| BssNI | GRCGYC | 2 cut(s) | 67, 115 |
| BssT1I | CCWWGG | 1 cut(s) | 336 |
| Bst2UI | CCWGG | 3 cut(s) | 55, 472, 990 |
| Bst4CI | ACNGT | 1 cut(s) | 388 |
| Bst6I | CTCTTC | 2 cut(s) | 171, 658 |
| BstACI | GRCGYC | 2 cut(s) | 67, 115 |
| BstAFI | CTTAAG | 1 cut(s) | 59 |
| BstAUI | TGTACA | 1 cut(s) | 717 |
| BstC8I | GCNNGC | 2 cut(s) | 424, 1016 |
| BstDEI | CTNAG | 2 cut(s) | 842, 914 |
| BstDSI | CCRYGG | 1 cut(s) | 312 |
| BstF5I | GGATG | 4 cut(s) | 187, 355, 573, 911 |
| BstKTI | GATC | 2 cut(s) | 223, 886 |
| BstMAI | GTCTC | 3 cut(s) | 58, 655, 832 |
| BstMBI | GATC | 2 cut(s) | 220, 883 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 98, 275, 369, 432, 945, 954 |
| BstNI | CCWGG | 3 cut(s) | 55, 472, 990 |
| BstNSI | RCATGY | 2 cut(s) | 576, 793 |
| BstSCI | CCNGG | 4 cut(s) | 53, 345, 470, 988 |
| BstSFI | CTRYAG | 3 cut(s) | 131, 433, 724 |
| BstV1I | GCAGC | 7 cut(s) | 60, 76, 287, 447, 626, 738, 969 |
| BstV2I | GAAGAC | 1 cut(s) | 388 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 337 |
| BsuRI | GGCC | 3 cut(s) | 341, 475, 1014 |
| BtgI | CCRYGG | 1 cut(s) | 312 |
| BtgZI | GCGATG | 1 cut(s) | 82 |
| BtsCI | GGATG | 4 cut(s) | 187, 355, 573, 911 |
| BtsIMutI | CAGTG | 1 cut(s) | 407 |
| Cac8I | GCNNGC | 2 cut(s) | 424, 1016 |
| Cfr13I | GGNCC | 5 cut(s) | 41, 109, 246, 321, 474 |
| CseI | GACGC | 3 cut(s) | 123, 144, 224 |
| Csp6I | GTAC | 1 cut(s) | 718 |
| CviAII | CATG | 6 cut(s) | 573, 593, 624, 790, 950, 1008 |
| CviQI | GTAC | 1 cut(s) | 718 |
| DdeI | CTNAG | 2 cut(s) | 842, 914 |
| DpnI | GATC | 2 cut(s) | 222, 885 |
| DpnII | GATC | 2 cut(s) | 220, 883 |
| EaeI | YGGCCR | 2 cut(s) | 339, 1012 |
| Eam1104I | CTCTTC | 2 cut(s) | 171, 658 |
| EarI | CTCTTC | 2 cut(s) | 171, 658 |
| Eco130I | CCWWGG | 1 cut(s) | 336 |
| Eco32I | GATATC | 1 cut(s) | 1030 |
| Eco47I | GGWCC | 4 cut(s) | 41, 109, 246, 321 |
| Eco57I | CTGAAG | 1 cut(s) | 189 |
| EcoRII | CCWGG | 3 cut(s) | 53, 470, 988 |
| EcoRV | GATATC | 1 cut(s) | 1030 |
| EcoT14I | CCWWGG | 1 cut(s) | 336 |
| EcoT22I | ATGCAT | 1 cut(s) | 612 |
| ErhI | CCWWGG | 1 cut(s) | 336 |
| Esp3I | CGTCTC | 1 cut(s) | 58 |
| FaeI | CATG | 6 cut(s) | 576, 596, 627, 793, 953, 1011 |
| FaqI | GGGAC | 3 cut(s) | 122, 374, 444 |
| FatI | CATG | 6 cut(s) | 572, 592, 623, 789, 949, 1007 |
| FauNDI | CATATG | 1 cut(s) | 1037 |
| FbaI | TGATCA | 1 cut(s) | 220 |
| FblI | GTMKAC | 1 cut(s) | 910 |
| Fnu4HI | GCNGC | 7 cut(s) | 49, 90, 276, 436, 640, 727, 958 |
| FokI | GGATG | 4 cut(s) | 194, 362, 580, 918 |
| Fsp4HI | GCNGC | 7 cut(s) | 49, 90, 276, 436, 640, 727, 958 |
| FspBI | CTAG | 1 cut(s) | 858 |
| GluI | GCNGC | 7 cut(s) | 49, 90, 276, 436, 640, 727, 958 |
| HaeIII | GGCC | 3 cut(s) | 341, 475, 1014 |
| HapII | CCGG | 1 cut(s) | 347 |
| HgaI | GACGC | 3 cut(s) | 123, 144, 224 |
| Hin1I | GRCGYC | 2 cut(s) | 67, 115 |
| Hin1II | CATG | 6 cut(s) | 576, 596, 627, 793, 953, 1011 |
| HinfI | GANTC | 8 cut(s) | 9, 149, 164, 191, 209, 377, 518, 812 |
| HpaII | CCGG | 1 cut(s) | 347 |
| HphI | GGTGA | 4 cut(s) | 35, 230, 606, 847 |
| Hpy166II | GTNNAC | 1 cut(s) | 911 |
| Hpy188I | TCNGA | 7 cut(s) | 163, 169, 196, 214, 246, 817, 917 |
| Hpy188III | TCNNGA | 4 cut(s) | 224, 515, 773, 803 |
| Hpy8I | GTNNAC | 1 cut(s) | 911 |
| Hpy99I | CGWCG | 2 cut(s) | 72, 117 |
| HpyAV | CCTTC | 3 cut(s) | 209, 436, 937 |
| HpyCH4III | ACNGT | 1 cut(s) | 388 |
| HpyCH4IV | ACGT | 1 cut(s) | 67 |
| HpyCH4V | TGCA | 7 cut(s) | 435, 558, 610, 642, 704, 726, 1035 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 98, 275, 369, 432, 945, 954 |
| HpyF3I | CTNAG | 2 cut(s) | 842, 914 |
| HpySE526I | ACGT | 1 cut(s) | 67 |
| Hsp92I | GRCGYC | 2 cut(s) | 67, 115 |
| Hsp92II | CATG | 6 cut(s) | 576, 596, 627, 793, 953, 1011 |
| Ksp22I | TGATCA | 1 cut(s) | 220 |
| Kzo9I | GATC | 2 cut(s) | 220, 883 |
| LmnI | GCTCC | 2 cut(s) | 431, 980 |
| Lsp1109I | GCAGC | 7 cut(s) | 60, 76, 287, 447, 626, 738, 969 |
| MaeI | CTAG | 1 cut(s) | 858 |
| MaeII | ACGT | 1 cut(s) | 67 |
| MalI | GATC | 2 cut(s) | 222, 885 |
| MboI | GATC | 2 cut(s) | 220, 883 |
| MhlI | GDGCHC | 2 cut(s) | 231, 357 |
| MlsI | TGGCCA | 1 cut(s) | 1014 |
| MluCI | AATT | 4 cut(s) | 522, 528, 665, 735 |
| MluNI | TGGCCA | 1 cut(s) | 1014 |
| MlyI | GAGTC | 1 cut(s) | 386 |
| MmeI | TCCRAC | 1 cut(s) | 1005 |
| Mox20I | TGGCCA | 1 cut(s) | 1014 |
| Mph1103I | ATGCAT | 1 cut(s) | 612 |
| MscI | TGGCCA | 1 cut(s) | 1014 |
| MseI | TTAA | 3 cut(s) | 60, 447, 750 |
| Msp20I | TGGCCA | 1 cut(s) | 1014 |
| MspCI | CTTAAG | 1 cut(s) | 59 |
| MspI | CCGG | 1 cut(s) | 347 |
| MspR9I | CCNGG | 4 cut(s) | 55, 347, 472, 990 |
| Mva1269I | GAATGC | 1 cut(s) | 610 |
| MvaI | CCWGG | 3 cut(s) | 55, 472, 990 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 98, 275, 369, 432, 945, 954 |
| NciI | CCSGG | 1 cut(s) | 347 |
| NdeI | CATATG | 1 cut(s) | 1037 |
| NdeII | GATC | 2 cut(s) | 220, 883 |
| NlaIII | CATG | 6 cut(s) | 576, 596, 627, 793, 953, 1011 |
| NlaIV | GGNNCC | 2 cut(s) | 110, 976 |
| NmeAIII | GCCGAG | 1 cut(s) | 143 |
| NsiI | ATGCAT | 1 cut(s) | 612 |
| NspI | RCATGY | 2 cut(s) | 576, 793 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 11 |
| PctI | GAATGC | 1 cut(s) | 610 |
| PfeI | GAWTC | 7 cut(s) | 9, 149, 164, 191, 209, 518, 812 |
| PflFI | GACNNNGTC | 1 cut(s) | 69 |
| PkrI | GCNGC | 7 cut(s) | 50, 91, 277, 437, 641, 728, 959 |
| PleI | GAGTC | 1 cut(s) | 385 |
| PpsI | GAGTC | 1 cut(s) | 385 |
| PsiI | TTATAA | 2 cut(s) | 486, 983 |
| Psp6I | CCWGG | 3 cut(s) | 53, 470, 988 |
| PspGI | CCWGG | 3 cut(s) | 53, 470, 988 |
| PspN4I | GGNNCC | 2 cut(s) | 110, 976 |
| PspPI | GGNCC | 5 cut(s) | 41, 109, 246, 321, 474 |
| PstI | CTGCAG | 2 cut(s) | 437, 728 |
| PsyI | GACNNNGTC | 1 cut(s) | 69 |
| RsaI | GTAC | 1 cut(s) | 719 |
| RsaNI | GTAC | 1 cut(s) | 718 |
| SaqAI | TTAA | 3 cut(s) | 60, 447, 750 |
| SatI | GCNGC | 7 cut(s) | 49, 90, 276, 436, 640, 727, 958 |
| Sau3AI | GATC | 2 cut(s) | 220, 883 |
| Sau96I | GGNCC | 5 cut(s) | 41, 109, 246, 321, 474 |
| SchI | GAGTC | 1 cut(s) | 386 |
| ScrFI | CCNGG | 4 cut(s) | 55, 347, 472, 990 |
| SduI | GDGCHC | 2 cut(s) | 231, 357 |
| SfcI | CTRYAG | 3 cut(s) | 131, 433, 724 |
| SinI | GGWCC | 4 cut(s) | 41, 109, 246, 321 |
| SmlI | CTYRAG | 2 cut(s) | 59, 1019 |
| SmoI | CTYRAG | 2 cut(s) | 59, 1019 |
| Sse9I | AATT | 4 cut(s) | 522, 528, 665, 735 |
| SsiI | CCGC | 3 cut(s) | 21, 273, 309 |
| SspMI | CTAG | 1 cut(s) | 858 |
| StyD4I | CCNGG | 4 cut(s) | 53, 345, 470, 988 |
| StyI | CCWWGG | 1 cut(s) | 336 |
| TaaI | ACNGT | 1 cut(s) | 388 |
| TaiI | ACGT | 1 cut(s) | 70 |
| TaqI | TCGA | 2 cut(s) | 255, 357 |
| TaqII | GACCGA | 2 cut(s) | 58, 126 |
| TasI | AATT | 4 cut(s) | 522, 528, 665, 735 |
| TatI | WGTACW | 1 cut(s) | 717 |
| TfiI | GAWTC | 7 cut(s) | 9, 149, 164, 191, 209, 518, 812 |
| Tru1I | TTAA | 3 cut(s) | 60, 447, 750 |
| Tru9I | TTAA | 3 cut(s) | 60, 447, 750 |
| TscAI | CASTG | 1 cut(s) | 407 |
| TseI | GCWGC | 7 cut(s) | 48, 89, 275, 435, 639, 726, 957 |
| TspDTI | ATGAA | 9 cut(s) | 42, 189, 198, 603, 609, 612, 825, 957, 996 |
| TspGWI | ACGGA | 1 cut(s) | 329 |
| TspRI | CASTG | 1 cut(s) | 407 |
| Tth111I | GACNNNGTC | 1 cut(s) | 69 |
| Vha464I | CTTAAG | 1 cut(s) | 59 |
| VpaK11BI | GGWCC | 4 cut(s) | 41, 109, 246, 321 |
| XapI | RAATTY | 1 cut(s) | 528 |
| XceI | RCATGY | 2 cut(s) | 576, 793 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 256, 407 |
| XmiI | GTMKAC | 1 cut(s) | 910 |
| XspI | CTAG | 1 cut(s) | 858 |
| ZraI | GACGTC | 1 cut(s) | 68 |
| Zsp2I | ATGCAT | 1 cut(s) | 612 |