E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 565 |
| AciI | CCGC | 1 cut(s) | 21 |
| AclI | AACGTT | 1 cut(s) | 311 |
| AclWI | GGATC | 7 cut(s) | 201, 208, 314, 327, 648, 1025, 1040 |
| AcoI | YGGCCR | 2 cut(s) | 1021, 1027 |
| AcsI | RAATTY | 1 cut(s) | 257 |
| AcuI | CTGAAG | 2 cut(s) | 731, 917 |
| AcyI | GRCGYC | 2 cut(s) | 111, 126 |
| AfaI | GTAC | 3 cut(s) | 403, 445, 544 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 72, 423, 528, 848, 953 |
| AgsI | TTSAA | 3 cut(s) | 90, 361, 1003 |
| AjiI | CACGTC | 1 cut(s) | 655 |
| AleI | CACNNNNGTG | 2 cut(s) | 419, 657 |
| AluBI | AGCT | 4 cut(s) | 476, 717, 822, 891 |
| AluI | AGCT | 4 cut(s) | 476, 717, 822, 891 |
| Alw26I | GTCTC | 5 cut(s) | 21, 110, 172, 238, 511 |
| AlwI | GGATC | 7 cut(s) | 201, 208, 314, 327, 648, 1025, 1040 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 188 |
| AoxI | GGCC | 2 cut(s) | 1021, 1027 |
| ApeKI | GCWGC | 1 cut(s) | 411 |
| ApoI | RAATTY | 1 cut(s) | 257 |
| AspS9I | GGNCC | 2 cut(s) | 41, 721 |
| AsuC2I | CCSGG | 2 cut(s) | 67, 843 |
| AsuHPI | GGTGA | 4 cut(s) | 50, 152, 580, 925 |
| AvaII | GGWCC | 2 cut(s) | 41, 721 |
| BamHI | GGATCC | 1 cut(s) | 319 |
| BauI | CACGAG | 1 cut(s) | 508 |
| BbsI | GAAGAC | 4 cut(s) | 114, 153, 159, 740 |
| BbvI | GCAGC | 1 cut(s) | 398 |
| BccI | CCATC | 2 cut(s) | 755, 947 |
| BceAI | ACGGC | 1 cut(s) | 849 |
| BcnI | CCSGG | 2 cut(s) | 67, 843 |
| BcoDI | GTCTC | 5 cut(s) | 21, 110, 172, 238, 511 |
| BfaI | CTAG | 2 cut(s) | 285, 501 |
| BfmI | CTRYAG | 1 cut(s) | 1007 |
| BfuAI | ACCTGC | 1 cut(s) | 565 |
| BisI | GCNGC | 1 cut(s) | 412 |
| BlsI | GCNGC | 1 cut(s) | 413 |
| Bme1390I | CCNGG | 2 cut(s) | 67, 843 |
| Bme18I | GGWCC | 2 cut(s) | 41, 721 |
| BmgBI | CACGTC | 1 cut(s) | 655 |
| BmgT120I | GGNCC | 2 cut(s) | 41, 721 |
| BmiI | GGNNCC | 1 cut(s) | 321 |
| BmrFI | CCNGG | 2 cut(s) | 67, 843 |
| BmrI | ACTGGG | 1 cut(s) | 636 |
| BmsI | GCATC | 2 cut(s) | 422, 506 |
| BmuI | ACTGGG | 1 cut(s) | 636 |
| BpiI | GAAGAC | 4 cut(s) | 114, 153, 159, 740 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 530, 562 |
| BpuMI | CCSGG | 2 cut(s) | 67, 843 |
| Bsa29I | ATCGAT | 1 cut(s) | 93 |
| BsaBI | GATNNNNATC | 3 cut(s) | 219, 368, 1016 |
| BsaHI | GRCGYC | 2 cut(s) | 111, 126 |
| BsaI | GGTCTC | 2 cut(s) | 110, 172 |
| BsaJI | CCNNGG | 1 cut(s) | 1024 |
| BsaWI | WCCGGW | 1 cut(s) | 137 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 72, 423, 528, 848, 953 |
| Bse1I | ACTGG | 1 cut(s) | 631 |
| Bse8I | GATNNNNATC | 3 cut(s) | 219, 368, 1016 |
| BseCI | ATCGAT | 1 cut(s) | 93 |
| BseDI | CCNNGG | 1 cut(s) | 1024 |
| BseGI | GGATG | 1 cut(s) | 943 |
| BseJI | GATNNNNATC | 3 cut(s) | 219, 368, 1016 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 72, 423, 528, 848, 953 |
| BseMII | CTCAG | 3 cut(s) | 27, 176, 180 |
| BseNI | ACTGG | 1 cut(s) | 631 |
| BseRI | GAGGAG | 2 cut(s) | 39, 254 |
| BseXI | GCAGC | 1 cut(s) | 398 |
| BsgI | GTGCAG | 1 cut(s) | 397 |
| Bsh1285I | CGRYCG | 1 cut(s) | 223 |
| BshFI | GGCC | 2 cut(s) | 1023, 1029 |
| BshVI | ATCGAT | 1 cut(s) | 93 |
| BsiEI | CGRYCG | 1 cut(s) | 223 |
| BsiSI | CCGG | 5 cut(s) | 67, 129, 138, 842, 1030 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 72, 423, 528, 848, 953 |
| BsmAI | GTCTC | 5 cut(s) | 21, 110, 172, 238, 511 |
| BsmBI | CGTCTC | 2 cut(s) | 21, 238 |
| BsnI | GGCC | 2 cut(s) | 1023, 1029 |
| Bso31I | GGTCTC | 2 cut(s) | 110, 172 |
| Bsp1407I | TGTACA | 1 cut(s) | 443 |
| Bsp143I | GATC | 9 cut(s) | 193, 200, 220, 319, 640, 676, 681, 1017, 1032 |
| Bsp19I | CCATGG | 1 cut(s) | 1024 |
| BspACI | CCGC | 1 cut(s) | 21 |
| BspANI | GGCC | 2 cut(s) | 1023, 1029 |
| BspCNI | CTCAG | 3 cut(s) | 26, 175, 181 |
| BspDI | ATCGAT | 1 cut(s) | 93 |
| BspHI | TCATGA | 1 cut(s) | 568 |
| BspLI | GGNNCC | 1 cut(s) | 321 |
| BspMAI | CTGCAG | 1 cut(s) | 1011 |
| BspMI | ACCTGC | 1 cut(s) | 565 |
| BspPI | GGATC | 7 cut(s) | 201, 208, 314, 327, 648, 1025, 1040 |
| BspTNI | GGTCTC | 2 cut(s) | 110, 172 |
| BsrGI | TGTACA | 1 cut(s) | 443 |
| BsrI | ACTGG | 1 cut(s) | 631 |
| BssECI | CCNNGG | 1 cut(s) | 1024 |
| BssMI | GATC | 9 cut(s) | 193, 200, 220, 319, 640, 676, 681, 1017, 1032 |
| BssNI | GRCGYC | 2 cut(s) | 111, 126 |
| BssSI | CACGAG | 1 cut(s) | 508 |
| BssT1I | CCWWGG | 1 cut(s) | 1024 |
| Bst2BI | CACGAG | 1 cut(s) | 508 |
| Bst4CI | ACNGT | 2 cut(s) | 560, 883 |
| BstACI | GRCGYC | 2 cut(s) | 111, 126 |
| BstAUI | TGTACA | 1 cut(s) | 443 |
| BstC8I | GCNNGC | 2 cut(s) | 353, 801 |
| BstDEI | CTNAG | 3 cut(s) | 13, 162, 189 |
| BstDSI | CCRYGG | 1 cut(s) | 1024 |
| BstF5I | GGATG | 1 cut(s) | 943 |
| BstKTI | GATC | 9 cut(s) | 196, 203, 223, 322, 643, 679, 684, 1020, 1035 |
| BstMAI | GTCTC | 5 cut(s) | 21, 110, 172, 238, 511 |
| BstMBI | GATC | 9 cut(s) | 193, 200, 220, 319, 640, 676, 681, 1017, 1032 |
| BstMCI | CGRYCG | 1 cut(s) | 223 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 216, 800 |
| BstNSI | RCATGY | 2 cut(s) | 770, 803 |
| BstSCI | CCNGG | 2 cut(s) | 65, 841 |
| BstSFI | CTRYAG | 1 cut(s) | 1007 |
| BstV1I | GCAGC | 1 cut(s) | 398 |
| BstV2I | GAAGAC | 4 cut(s) | 114, 153, 159, 740 |
| BstX2I | RGATCY | 3 cut(s) | 319, 640, 1032 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 626, 718 |
| BstYI | RGATCY | 3 cut(s) | 319, 640, 1032 |
| Bsu15I | ATCGAT | 1 cut(s) | 93 |
| BsuRI | GGCC | 2 cut(s) | 1023, 1029 |
| BsuTUI | ATCGAT | 1 cut(s) | 93 |
| BtgI | CCRYGG | 1 cut(s) | 1024 |
| BtgZI | GCGATG | 1 cut(s) | 225 |
| BtrI | CACGTC | 1 cut(s) | 655 |
| BtsCI | GGATG | 1 cut(s) | 943 |
| BtsIMutI | CAGTG | 3 cut(s) | 383, 624, 820 |
| BveI | ACCTGC | 1 cut(s) | 565 |
| Cac8I | GCNNGC | 2 cut(s) | 353, 801 |
| CaiI | CAGNNNCTG | 1 cut(s) | 188 |
| CciI | TCATGA | 1 cut(s) | 568 |
| Cfr13I | GGNCC | 2 cut(s) | 41, 721 |
| ClaI | ATCGAT | 1 cut(s) | 93 |
| CseI | GACGC | 2 cut(s) | 119, 134 |
| Csp6I | GTAC | 3 cut(s) | 402, 444, 543 |
| CviAII | CATG | 6 cut(s) | 569, 767, 800, 861, 961, 1025 |
| CviJI | RGCY | 9 cut(s) | 355, 398, 476, 717, 794, 822, 891, 1023, 1029 |
| CviKI_1 | RGCY | 9 cut(s) | 355, 398, 476, 717, 794, 822, 891, 1023, 1029 |
| CviQI | GTAC | 3 cut(s) | 402, 444, 543 |
| DdeI | CTNAG | 3 cut(s) | 13, 162, 189 |
| DpnI | GATC | 9 cut(s) | 195, 202, 222, 321, 642, 678, 683, 1019, 1034 |
| DpnII | GATC | 9 cut(s) | 193, 200, 220, 319, 640, 676, 681, 1017, 1032 |
| EaeI | YGGCCR | 2 cut(s) | 1021, 1027 |
| Eco130I | CCWWGG | 1 cut(s) | 1024 |
| Eco31I | GGTCTC | 2 cut(s) | 110, 172 |
| Eco47I | GGWCC | 2 cut(s) | 41, 721 |
| Eco57I | CTGAAG | 2 cut(s) | 731, 917 |
| EcoT14I | CCWWGG | 1 cut(s) | 1024 |
| ErhI | CCWWGG | 1 cut(s) | 1024 |
| Esp3I | CGTCTC | 2 cut(s) | 21, 238 |
| FaeI | CATG | 6 cut(s) | 572, 770, 803, 864, 964, 1028 |
| FatI | CATG | 6 cut(s) | 568, 766, 799, 860, 960, 1024 |
| Fnu4HI | GCNGC | 1 cut(s) | 412 |
| FokI | GGATG | 1 cut(s) | 930 |
| Fsp4HI | GCNGC | 1 cut(s) | 412 |
| FspBI | CTAG | 2 cut(s) | 285, 501 |
| GluI | GCNGC | 1 cut(s) | 412 |
| HaeIII | GGCC | 2 cut(s) | 1023, 1029 |
| HapII | CCGG | 5 cut(s) | 67, 129, 138, 842, 1030 |
| HgaI | GACGC | 2 cut(s) | 119, 134 |
| Hin1I | GRCGYC | 2 cut(s) | 111, 126 |
| Hin1II | CATG | 6 cut(s) | 572, 770, 803, 864, 964, 1028 |
| HinfI | GANTC | 8 cut(s) | 9, 166, 185, 293, 364, 504, 967, 1003 |
| HpaII | CCGG | 5 cut(s) | 67, 129, 138, 842, 1030 |
| HphI | GGTGA | 4 cut(s) | 50, 152, 580, 925 |
| Hpy166II | GTNNAC | 1 cut(s) | 444 |
| Hpy188I | TCNGA | 5 cut(s) | 124, 165, 190, 273, 681 |
| Hpy188III | TCNNGA | 8 cut(s) | 224, 323, 332, 501, 569, 749, 947, 1047 |
| Hpy8I | GTNNAC | 1 cut(s) | 444 |
| Hpy99I | CGWCG | 3 cut(s) | 35, 128, 442 |
| HpyAV | CCTTC | 4 cut(s) | 32, 96, 478, 981 |
| HpyCH4III | ACNGT | 2 cut(s) | 560, 883 |
| HpyCH4IV | ACGT | 2 cut(s) | 311, 654 |
| HpyCH4V | TGCA | 6 cut(s) | 414, 471, 534, 799, 830, 1009 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 216, 800 |
| HpyF3I | CTNAG | 3 cut(s) | 13, 162, 189 |
| HpySE526I | ACGT | 2 cut(s) | 311, 654 |
| Hsp92I | GRCGYC | 2 cut(s) | 111, 126 |
| Hsp92II | CATG | 6 cut(s) | 572, 770, 803, 864, 964, 1028 |
| Kzo9I | GATC | 9 cut(s) | 193, 200, 220, 319, 640, 676, 681, 1017, 1032 |
| Lsp1109I | GCAGC | 1 cut(s) | 398 |
| LweI | GCATC | 2 cut(s) | 422, 506 |
| MaeI | CTAG | 2 cut(s) | 285, 501 |
| MaeII | ACGT | 2 cut(s) | 311, 654 |
| MaeIII | GTNAC | 3 cut(s) | 47, 307, 931 |
| MalI | GATC | 9 cut(s) | 195, 202, 222, 321, 642, 678, 683, 1019, 1034 |
| MboI | GATC | 9 cut(s) | 193, 200, 220, 319, 640, 676, 681, 1017, 1032 |
| MflI | RGATCY | 3 cut(s) | 319, 640, 1032 |
| MluCI | AATT | 2 cut(s) | 257, 741 |
| MlyI | GAGTC | 1 cut(s) | 513 |
| MmeI | TCCRAC | 1 cut(s) | 147 |
| MnlI | CCTC | 9 cut(s) | 17, 130, 184, 191, 232, 309, 417, 556, 734 |
| MseI | TTAA | 1 cut(s) | 707 |
| MslI | CAYNNNNRTG | 3 cut(s) | 419, 657, 672 |
| MspI | CCGG | 5 cut(s) | 67, 129, 138, 842, 1030 |
| MspR9I | CCNGG | 2 cut(s) | 67, 843 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 216, 800 |
| NciI | CCSGG | 2 cut(s) | 67, 843 |
| NcoI | CCATGG | 1 cut(s) | 1024 |
| NdeII | GATC | 9 cut(s) | 193, 200, 220, 319, 640, 676, 681, 1017, 1032 |
| NlaIII | CATG | 6 cut(s) | 572, 770, 803, 864, 964, 1028 |
| NlaIV | GGNNCC | 1 cut(s) | 321 |
| NmeAIII | GCCGAG | 1 cut(s) | 139 |
| NmuCI | GTSAC | 1 cut(s) | 931 |
| NspI | RCATGY | 2 cut(s) | 770, 803 |
| OliI | CACNNNNGTG | 2 cut(s) | 419, 657 |
| PaeI | GCATGC | 1 cut(s) | 803 |
| PagI | TCATGA | 1 cut(s) | 568 |
| PfeI | GAWTC | 7 cut(s) | 9, 166, 185, 293, 364, 967, 1003 |
| PfoI | TCCNGGA | 1 cut(s) | 65 |
| PkrI | GCNGC | 1 cut(s) | 413 |
| Ple19I | CGATCG | 1 cut(s) | 223 |
| PleI | GAGTC | 1 cut(s) | 512 |
| PpsI | GAGTC | 1 cut(s) | 512 |
| Psp1406I | AACGTT | 1 cut(s) | 311 |
| PspN4I | GGNNCC | 1 cut(s) | 321 |
| PspPI | GGNCC | 2 cut(s) | 41, 721 |
| PstI | CTGCAG | 1 cut(s) | 1011 |
| PstNI | CAGNNNCTG | 1 cut(s) | 188 |
| PsuI | RGATCY | 3 cut(s) | 319, 640, 1032 |
| PvuI | CGATCG | 1 cut(s) | 223 |
| RsaI | GTAC | 3 cut(s) | 403, 445, 544 |
| RsaNI | GTAC | 3 cut(s) | 402, 444, 543 |
| RseI | CAYNNNNRTG | 3 cut(s) | 419, 657, 672 |
| SaqAI | TTAA | 1 cut(s) | 707 |
| SatI | GCNGC | 1 cut(s) | 412 |
| Sau3AI | GATC | 9 cut(s) | 193, 200, 220, 319, 640, 676, 681, 1017, 1032 |
| Sau96I | GGNCC | 2 cut(s) | 41, 721 |
| SchI | GAGTC | 1 cut(s) | 513 |
| ScrFI | CCNGG | 2 cut(s) | 67, 843 |
| SfaNI | GCATC | 2 cut(s) | 422, 506 |
| SfcI | CTRYAG | 1 cut(s) | 1007 |
| SinI | GGWCC | 2 cut(s) | 41, 721 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 419, 657, 672 |
| SphI | GCATGC | 1 cut(s) | 803 |
| Sse9I | AATT | 2 cut(s) | 257, 741 |
| SsiI | CCGC | 1 cut(s) | 21 |
| SspMI | CTAG | 2 cut(s) | 285, 501 |
| StyD4I | CCNGG | 2 cut(s) | 65, 841 |
| StyI | CCWWGG | 1 cut(s) | 1024 |
| TaaI | ACNGT | 2 cut(s) | 560, 883 |
| TaiI | ACGT | 2 cut(s) | 314, 657 |
| TaqI | TCGA | 2 cut(s) | 93, 196 |
| TasI | AATT | 2 cut(s) | 257, 741 |
| TatI | WGTACW | 2 cut(s) | 401, 443 |
| TfiI | GAWTC | 7 cut(s) | 9, 166, 185, 293, 364, 967, 1003 |
| Tru1I | TTAA | 1 cut(s) | 707 |
| Tru9I | TTAA | 1 cut(s) | 707 |
| TscAI | CASTG | 3 cut(s) | 383, 631, 820 |
| TseFI | GTSAC | 1 cut(s) | 931 |
| TseI | GCWGC | 1 cut(s) | 411 |
| Tsp45I | GTSAC | 1 cut(s) | 931 |
| TspDTI | ATGAA | 5 cut(s) | 111, 117, 949, 954, 1031 |
| TspGWI | ACGGA | 2 cut(s) | 48, 647 |
| TspRI | CASTG | 3 cut(s) | 383, 631, 820 |
| VpaK11BI | GGWCC | 2 cut(s) | 41, 721 |
| XapI | RAATTY | 1 cut(s) | 257 |
| XbaI | TCTAGA | 1 cut(s) | 500 |
| XceI | RCATGY | 2 cut(s) | 770, 803 |
| XspI | CTAG | 2 cut(s) | 285, 501 |