E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 572 |
| AatII | GACGTC | 1 cut(s) | 13 |
| Acc36I | ACCTGC | 1 cut(s) | 572 |
| AccB1I | GGYRCC | 2 cut(s) | 1217, 1239 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 164, 380 |
| AccIII | TCCGGA | 1 cut(s) | 214 |
| AciI | CCGC | 1 cut(s) | 1136 |
| AclWI | GGATC | 5 cut(s) | 111, 206, 609, 622, 947 |
| AcoI | YGGCCR | 1 cut(s) | 54 |
| AcsI | RAATTY | 1 cut(s) | 970 |
| AcyI | GRCGYC | 2 cut(s) | 10, 1218 |
| AfaI | GTAC | 1 cut(s) | 683 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 164, 380, 767 |
| AgsI | TTSAA | 5 cut(s) | 82, 450, 722, 1018, 1269 |
| AluBI | AGCT | 5 cut(s) | 428, 479, 987, 1002, 1168 |
| AluI | AGCT | 5 cut(s) | 428, 479, 987, 1002, 1168 |
| Alw26I | GTCTC | 3 cut(s) | 743, 787, 1040 |
| AlwI | GGATC | 5 cut(s) | 111, 206, 609, 622, 947 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 25 |
| Ama87I | CYCGRG | 2 cut(s) | 1155, 1169 |
| Aor13HI | TCCGGA | 1 cut(s) | 214 |
| AoxI | GGCC | 2 cut(s) | 54, 630 |
| ApeKI | GCWGC | 4 cut(s) | 425, 567, 687, 771 |
| ApoI | RAATTY | 1 cut(s) | 970 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 845, 877 |
| AspLEI | GCGC | 2 cut(s) | 1126, 1220 |
| AspS9I | GGNCC | 2 cut(s) | 401, 630 |
| AsuC2I | CCSGG | 1 cut(s) | 629 |
| AsuHPI | GGTGA | 3 cut(s) | 137, 988, 1267 |
| AvaI | CYCGRG | 2 cut(s) | 1155, 1169 |
| AvaII | GGWCC | 1 cut(s) | 401 |
| AxyI | CCTNAGG | 1 cut(s) | 1251 |
| BaeGI | GKGCMC | 1 cut(s) | 1242 |
| BamHI | GGATCC | 1 cut(s) | 614 |
| BanI | GGYRCC | 2 cut(s) | 1217, 1239 |
| BanII | GRGCYC | 1 cut(s) | 510 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 82, 114 |
| BauI | CACGAG | 3 cut(s) | 696, 1040, 1083 |
| BbsI | GAAGAC | 3 cut(s) | 243, 309, 517 |
| BbvI | GCAGC | 4 cut(s) | 437, 554, 674, 758 |
| BccI | CCATC | 9 cut(s) | 128, 158, 383, 424, 524, 886, 920, 1032, 1168 |
| BceAI | ACGGC | 2 cut(s) | 617, 700 |
| BciVI | GTATCC | 1 cut(s) | 386 |
| BclI | TGATCA | 1 cut(s) | 535 |
| BcnI | CCSGG | 1 cut(s) | 629 |
| BcoDI | GTCTC | 3 cut(s) | 743, 787, 1040 |
| BfaI | CTAG | 2 cut(s) | 230, 365 |
| BfoI | RGCGCY | 1 cut(s) | 1221 |
| BfuAI | ACCTGC | 1 cut(s) | 572 |
| BfuI | GTATCC | 1 cut(s) | 386 |
| BisI | GCNGC | 4 cut(s) | 426, 568, 688, 772 |
| BlsI | GCNGC | 4 cut(s) | 427, 569, 689, 773 |
| Bme1390I | CCNGG | 1 cut(s) | 629 |
| Bme18I | GGWCC | 1 cut(s) | 401 |
| BmeT110I | CYCGRG | 2 cut(s) | 1155, 1169 |
| BmgT120I | GGNCC | 2 cut(s) | 401, 630 |
| BmiI | GGNNCC | 3 cut(s) | 616, 1219, 1241 |
| BmrFI | CCNGG | 1 cut(s) | 629 |
| BpiI | GAAGAC | 3 cut(s) | 243, 309, 517 |
| BpmI | CTGGAG | 1 cut(s) | 97 |
| BpuMI | CCSGG | 1 cut(s) | 629 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1178 |
| BsaHI | GRCGYC | 2 cut(s) | 10, 1218 |
| BsaWI | WCCGGW | 1 cut(s) | 214 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 833, 863 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 164, 380, 767 |
| Bse1I | ACTGG | 2 cut(s) | 529, 1119 |
| Bse21I | CCTNAGG | 1 cut(s) | 1251 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1178 |
| BseAI | TCCGGA | 1 cut(s) | 214 |
| BseGI | GGATG | 5 cut(s) | 169, 416, 516, 1115, 1165 |
| BseJI | GATNNNNATC | 1 cut(s) | 1178 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 164, 380, 767 |
| BseMII | CTCAG | 4 cut(s) | 17, 462, 1046, 1265 |
| BseNI | ACTGG | 2 cut(s) | 529, 1119 |
| BseRI | GAGGAG | 1 cut(s) | 1058 |
| BseSI | GKGCMC | 1 cut(s) | 1242 |
| BseX3I | CGGCCG | 1 cut(s) | 54 |
| BseXI | GCAGC | 4 cut(s) | 437, 554, 674, 758 |
| BseYI | CCCAGC | 1 cut(s) | 998 |
| BsgI | GTGCAG | 1 cut(s) | 553 |
| Bsh1285I | CGRYCG | 1 cut(s) | 57 |
| BshFI | GGCC | 2 cut(s) | 56, 632 |
| BshNI | GGYRCC | 2 cut(s) | 1217, 1239 |
| BsiEI | CGRYCG | 1 cut(s) | 57 |
| BsiHKCI | CYCGRG | 2 cut(s) | 1155, 1169 |
| BsiSI | CCGG | 2 cut(s) | 215, 628 |
| BslFI | GGGAC | 1 cut(s) | 1232 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 164, 380, 767 |
| BsmAI | GTCTC | 3 cut(s) | 743, 787, 1040 |
| BsmFI | GGGAC | 1 cut(s) | 1232 |
| BsnI | GGCC | 2 cut(s) | 56, 632 |
| BsoBI | CYCGRG | 2 cut(s) | 1155, 1169 |
| Bsp1286I | GDGCHC | 2 cut(s) | 510, 1242 |
| Bsp13I | TCCGGA | 1 cut(s) | 214 |
| Bsp143I | GATC | 9 cut(s) | 29, 116, 172, 211, 277, 535, 614, 952, 961 |
| BspACI | CCGC | 1 cut(s) | 1136 |
| BspANI | GGCC | 2 cut(s) | 56, 632 |
| BspCNI | CTCAG | 4 cut(s) | 18, 463, 1047, 1264 |
| BspEI | TCCGGA | 1 cut(s) | 214 |
| BspHI | TCATGA | 1 cut(s) | 1230 |
| BspLI | GGNNCC | 3 cut(s) | 616, 1219, 1241 |
| BspMI | ACCTGC | 1 cut(s) | 572 |
| BspPI | GGATC | 5 cut(s) | 111, 206, 609, 622, 947 |
| BspQI | GCTCTTC | 2 cut(s) | 246, 312 |
| BspT107I | GGYRCC | 2 cut(s) | 1217, 1239 |
| BsrI | ACTGG | 2 cut(s) | 529, 1119 |
| BssMI | GATC | 9 cut(s) | 29, 116, 172, 211, 277, 535, 614, 952, 961 |
| BssNI | GRCGYC | 2 cut(s) | 10, 1218 |
| BssSI | CACGAG | 3 cut(s) | 696, 1040, 1083 |
| Bst2BI | CACGAG | 3 cut(s) | 696, 1040, 1083 |
| Bst4CI | ACNGT | 1 cut(s) | 405 |
| Bst6I | CTCTTC | 5 cut(s) | 189, 246, 255, 312, 321 |
| BstACI | GRCGYC | 2 cut(s) | 10, 1218 |
| BstC8I | GCNNGC | 1 cut(s) | 477 |
| BstDEI | CTNAG | 5 cut(s) | 26, 471, 983, 1055, 1251 |
| BstF5I | GGATG | 5 cut(s) | 169, 416, 516, 1115, 1165 |
| BstH2I | RGCGCY | 1 cut(s) | 1221 |
| BstHHI | GCGC | 2 cut(s) | 1126, 1220 |
| BstKTI | GATC | 9 cut(s) | 32, 119, 175, 214, 280, 538, 617, 955, 964 |
| BstMAI | GTCTC | 3 cut(s) | 743, 787, 1040 |
| BstMBI | GATC | 9 cut(s) | 29, 116, 172, 211, 277, 535, 614, 952, 961 |
| BstMCI | CGRYCG | 1 cut(s) | 57 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 481, 498, 564 |
| BstSCI | CCNGG | 1 cut(s) | 627 |
| BstSLI | GKGCMC | 1 cut(s) | 1242 |
| BstV1I | GCAGC | 4 cut(s) | 437, 554, 674, 758 |
| BstV2I | GAAGAC | 3 cut(s) | 243, 309, 517 |
| BstX2I | RGATCY | 3 cut(s) | 116, 211, 614 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1006 |
| BstYI | RGATCY | 3 cut(s) | 116, 211, 614 |
| BstZI | CGGCCG | 1 cut(s) | 54 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1251 |
| BsuI | GTATCC | 1 cut(s) | 386 |
| BsuRI | GGCC | 2 cut(s) | 56, 632 |
| BtsCI | GGATG | 5 cut(s) | 169, 416, 516, 1115, 1165 |
| BtsIMutI | CAGTG | 2 cut(s) | 536, 1126 |
| BveI | ACCTGC | 1 cut(s) | 572 |
| Cac8I | GCNNGC | 1 cut(s) | 477 |
| CaiI | CAGNNNCTG | 1 cut(s) | 25 |
| CciI | TCATGA | 1 cut(s) | 1230 |
| CfoI | GCGC | 2 cut(s) | 1126, 1220 |
| Cfr13I | GGNCC | 2 cut(s) | 401, 630 |
| Csp6I | GTAC | 1 cut(s) | 682 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 438, 473 |
| CviAII | CATG | 8 cut(s) | 47, 443, 725, 756, 824, 1006, 1139, 1231 |
| CviQI | GTAC | 1 cut(s) | 682 |
| DdeI | CTNAG | 5 cut(s) | 26, 471, 983, 1055, 1251 |
| DinI | GGCGCC | 1 cut(s) | 1219 |
| DpnI | GATC | 9 cut(s) | 31, 118, 174, 213, 279, 537, 616, 954, 963 |
| DpnII | GATC | 9 cut(s) | 29, 116, 172, 211, 277, 535, 614, 952, 961 |
| EaeI | YGGCCR | 1 cut(s) | 54 |
| EagI | CGGCCG | 1 cut(s) | 54 |
| Eam1104I | CTCTTC | 5 cut(s) | 189, 246, 255, 312, 321 |
| EarI | CTCTTC | 5 cut(s) | 189, 246, 255, 312, 321 |
| EclXI | CGGCCG | 1 cut(s) | 54 |
| Eco24I | GRGCYC | 1 cut(s) | 510 |
| Eco47I | GGWCC | 1 cut(s) | 401 |
| Eco52I | CGGCCG | 1 cut(s) | 54 |
| Eco81I | CCTNAGG | 1 cut(s) | 1251 |
| Eco88I | CYCGRG | 2 cut(s) | 1155, 1169 |
| EcoT38I | GRGCYC | 1 cut(s) | 510 |
| EgeI | GGCGCC | 1 cut(s) | 1219 |
| EheI | GGCGCC | 1 cut(s) | 1219 |
| FaeI | CATG | 8 cut(s) | 50, 446, 728, 759, 827, 1009, 1142, 1234 |
| FaqI | GGGAC | 1 cut(s) | 1232 |
| FatI | CATG | 8 cut(s) | 46, 442, 724, 755, 823, 1005, 1138, 1230 |
| FauI | CCCGC | 1 cut(s) | 1143 |
| FbaI | TGATCA | 1 cut(s) | 535 |
| Fnu4HI | GCNGC | 4 cut(s) | 426, 568, 688, 772 |
| FokI | GGATG | 5 cut(s) | 176, 403, 503, 1102, 1172 |
| FriOI | GRGCYC | 1 cut(s) | 510 |
| Fsp4HI | GCNGC | 4 cut(s) | 426, 568, 688, 772 |
| FspBI | CTAG | 2 cut(s) | 230, 365 |
| GlaI | GCGC | 2 cut(s) | 1125, 1219 |
| GluI | GCNGC | 4 cut(s) | 426, 568, 688, 772 |
| GsaI | CCCAGC | 1 cut(s) | 1002 |
| GsuI | CTGGAG | 1 cut(s) | 97 |
| HaeII | RGCGCY | 1 cut(s) | 1221 |
| HaeIII | GGCC | 2 cut(s) | 56, 632 |
| HapII | CCGG | 2 cut(s) | 215, 628 |
| HhaI | GCGC | 2 cut(s) | 1126, 1220 |
| Hin1I | GRCGYC | 2 cut(s) | 10, 1218 |
| Hin1II | CATG | 8 cut(s) | 50, 446, 728, 759, 827, 1009, 1142, 1234 |
| Hin6I | GCGC | 2 cut(s) | 1124, 1218 |
| HinP1I | GCGC | 2 cut(s) | 1124, 1218 |
| HinfI | GANTC | 6 cut(s) | 16, 50, 650, 979, 1151, 1245 |
| HpaII | CCGG | 2 cut(s) | 215, 628 |
| HphI | GGTGA | 3 cut(s) | 137, 988, 1267 |
| Hpy188I | TCNGA | 4 cut(s) | 15, 27, 614, 1056 |
| Hpy99I | CGWCG | 4 cut(s) | 62, 638, 857, 860 |
| HpyAV | CCTTC | 3 cut(s) | 194, 392, 1208 |
| HpyCH4III | ACNGT | 1 cut(s) | 405 |
| HpyCH4IV | ACGT | 1 cut(s) | 10 |
| HpyCH4V | TGCA | 7 cut(s) | 151, 425, 570, 659, 690, 836, 1262 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 481, 498, 564 |
| HpyF3I | CTNAG | 5 cut(s) | 26, 471, 983, 1055, 1251 |
| HpySE526I | ACGT | 1 cut(s) | 10 |
| Hsp92I | GRCGYC | 2 cut(s) | 10, 1218 |
| Hsp92II | CATG | 8 cut(s) | 50, 446, 728, 759, 827, 1009, 1142, 1234 |
| HspAI | GCGC | 2 cut(s) | 1124, 1218 |
| KasI | GGCGCC | 1 cut(s) | 1217 |
| Kpn2I | TCCGGA | 1 cut(s) | 214 |
| Ksp22I | TGATCA | 1 cut(s) | 535 |
| Kzo9I | GATC | 9 cut(s) | 29, 116, 172, 211, 277, 535, 614, 952, 961 |
| LguI | GCTCTTC | 2 cut(s) | 246, 312 |
| LmnI | GCTCC | 2 cut(s) | 563, 779 |
| Lsp1109I | GCAGC | 4 cut(s) | 437, 554, 674, 758 |
| MaeI | CTAG | 2 cut(s) | 230, 365 |
| MaeII | ACGT | 1 cut(s) | 10 |
| MaeIII | GTNAC | 4 cut(s) | 182, 545, 976, 1069 |
| MalI | GATC | 9 cut(s) | 31, 118, 174, 213, 279, 537, 616, 954, 963 |
| MboI | GATC | 9 cut(s) | 29, 116, 172, 211, 277, 535, 614, 952, 961 |
| MfeI | CAATTG | 2 cut(s) | 146, 654 |
| MflI | RGATCY | 3 cut(s) | 116, 211, 614 |
| MhlI | GDGCHC | 2 cut(s) | 510, 1242 |
| Mly113I | GGCGCC | 1 cut(s) | 1218 |
| MlyI | GAGTC | 3 cut(s) | 44, 973, 1254 |
| MroI | TCCGGA | 1 cut(s) | 214 |
| MseI | TTAA | 5 cut(s) | 579, 884, 1035, 1061, 1130 |
| MspA1I | CMGCKG | 1 cut(s) | 428 |
| MspI | CCGG | 2 cut(s) | 215, 628 |
| MspR9I | CCNGG | 1 cut(s) | 629 |
| MunI | CAATTG | 2 cut(s) | 146, 654 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 481, 498, 564 |
| NarI | GGCGCC | 1 cut(s) | 1218 |
| NciI | CCSGG | 1 cut(s) | 629 |
| NdeII | GATC | 9 cut(s) | 29, 116, 172, 211, 277, 535, 614, 952, 961 |
| NlaIII | CATG | 8 cut(s) | 50, 446, 728, 759, 827, 1009, 1142, 1234 |
| NlaIV | GGNNCC | 3 cut(s) | 616, 1219, 1241 |
| NmeAIII | GCCGAG | 1 cut(s) | 32 |
| NmuCI | GTSAC | 3 cut(s) | 182, 545, 976 |
| PaeR7I | CTCGAG | 1 cut(s) | 1169 |
| PagI | TCATGA | 1 cut(s) | 1230 |
| PaqCI | CACCTGC | 1 cut(s) | 572 |
| PciSI | GCTCTTC | 2 cut(s) | 246, 312 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 852, 957 |
| PfeI | GAWTC | 3 cut(s) | 16, 650, 1151 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 164, 380 |
| PkrI | GCNGC | 4 cut(s) | 427, 569, 689, 773 |
| PleI | GAGTC | 3 cut(s) | 44, 973, 1253 |
| PluTI | GGCGCC | 1 cut(s) | 1221 |
| PpsI | GAGTC | 3 cut(s) | 44, 973, 1253 |
| PspFI | CCCAGC | 1 cut(s) | 998 |
| PspN4I | GGNNCC | 3 cut(s) | 616, 1219, 1241 |
| PspPI | GGNCC | 2 cut(s) | 401, 630 |
| PstNI | CAGNNNCTG | 1 cut(s) | 25 |
| PsuI | RGATCY | 3 cut(s) | 116, 211, 614 |
| PvuII | CAGCTG | 1 cut(s) | 428 |
| RsaI | GTAC | 1 cut(s) | 683 |
| RsaNI | GTAC | 1 cut(s) | 682 |
| SapI | GCTCTTC | 2 cut(s) | 246, 312 |
| SaqAI | TTAA | 5 cut(s) | 579, 884, 1035, 1061, 1130 |
| SatI | GCNGC | 4 cut(s) | 426, 568, 688, 772 |
| Sau3AI | GATC | 9 cut(s) | 29, 116, 172, 211, 277, 535, 614, 952, 961 |
| Sau96I | GGNCC | 2 cut(s) | 401, 630 |
| SchI | GAGTC | 3 cut(s) | 44, 973, 1254 |
| ScrFI | CCNGG | 1 cut(s) | 629 |
| SduI | GDGCHC | 2 cut(s) | 510, 1242 |
| SfoI | GGCGCC | 1 cut(s) | 1219 |
| Sfr274I | CTCGAG | 1 cut(s) | 1169 |
| SinI | GGWCC | 1 cut(s) | 401 |
| SlaI | CTCGAG | 1 cut(s) | 1169 |
| SmlI | CTYRAG | 1 cut(s) | 1169 |
| SmoI | CTYRAG | 1 cut(s) | 1169 |
| SsiI | CCGC | 1 cut(s) | 1136 |
| SspDI | GGCGCC | 1 cut(s) | 1217 |
| SspI | AATATT | 1 cut(s) | 454 |
| SspMI | CTAG | 2 cut(s) | 230, 365 |
| StyD4I | CCNGG | 1 cut(s) | 627 |
| TaaI | ACNGT | 1 cut(s) | 405 |
| TaiI | ACGT | 1 cut(s) | 13 |
| TaqI | TCGA | 7 cut(s) | 175, 486, 636, 913, 960, 1149, 1170 |
| TaqII | GACCGA | 1 cut(s) | 447 |
| TfiI | GAWTC | 3 cut(s) | 16, 650, 1151 |
| Tru1I | TTAA | 5 cut(s) | 579, 884, 1035, 1061, 1130 |
| Tru9I | TTAA | 5 cut(s) | 579, 884, 1035, 1061, 1130 |
| TscAI | CASTG | 2 cut(s) | 536, 1126 |
| TseFI | GTSAC | 3 cut(s) | 182, 545, 976 |
| TseI | GCWGC | 4 cut(s) | 425, 567, 687, 771 |
| Tsp45I | GTSAC | 3 cut(s) | 182, 545, 976 |
| TspGWI | ACGGA | 3 cut(s) | 146, 282, 348 |
| TspRI | CASTG | 2 cut(s) | 536, 1126 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 164, 380 |
| VpaK11BI | GGWCC | 1 cut(s) | 401 |
| XapI | RAATTY | 1 cut(s) | 970 |
| XhoI | CTCGAG | 1 cut(s) | 1169 |
| XspI | CTAG | 2 cut(s) | 230, 365 |
| ZraI | GACGTC | 1 cut(s) | 11 |