E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 331 |
| Acc36I | ACCTGC | 1 cut(s) | 670 |
| AciI | CCGC | 1 cut(s) | 52 |
| AclI | AACGTT | 2 cut(s) | 80, 435 |
| AcuI | CTGAAG | 1 cut(s) | 373 |
| AfaI | GTAC | 1 cut(s) | 514 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 344 |
| AgsI | TTSAA | 7 cut(s) | 122, 366, 469, 542, 604, 699, 733 |
| AjnI | CCWGG | 1 cut(s) | 91 |
| AluBI | AGCT | 5 cut(s) | 170, 442, 483, 570, 585 |
| AluI | AGCT | 5 cut(s) | 170, 442, 483, 570, 585 |
| Alw26I | GTCTC | 1 cut(s) | 644 |
| AoxI | GGCC | 2 cut(s) | 220, 246 |
| ApeKI | GCWGC | 3 cut(s) | 183, 264, 348 |
| AseI | ATTAAT | 1 cut(s) | 195 |
| AspLEI | GCGC | 1 cut(s) | 332 |
| AspS9I | GGNCC | 2 cut(s) | 221, 246 |
| AsuHPI | GGTGA | 2 cut(s) | 289, 667 |
| BbvI | GCAGC | 3 cut(s) | 170, 251, 335 |
| BciT130I | CCWGG | 1 cut(s) | 93 |
| BclI | TGATCA | 1 cut(s) | 151 |
| BcoDI | GTCTC | 1 cut(s) | 644 |
| BfmI | CTRYAG | 1 cut(s) | 677 |
| BfuAI | ACCTGC | 1 cut(s) | 670 |
| BisI | GCNGC | 3 cut(s) | 184, 265, 349 |
| BlsI | GCNGC | 3 cut(s) | 185, 266, 350 |
| Bme1390I | CCNGG | 1 cut(s) | 93 |
| BmgT120I | GGNCC | 2 cut(s) | 221, 246 |
| BmiI | GGNNCC | 2 cut(s) | 64, 223 |
| BmrFI | CCNGG | 1 cut(s) | 93 |
| BmrI | ACTGGG | 1 cut(s) | 452 |
| BmuI | ACTGGG | 1 cut(s) | 452 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 29, 61 |
| BsaJI | CCNNGG | 1 cut(s) | 91 |
| BsaWI | WCCGGW | 1 cut(s) | 274 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 344 |
| Bse1I | ACTGG | 1 cut(s) | 458 |
| BseBI | CCWGG | 1 cut(s) | 93 |
| BseDI | CCNNGG | 1 cut(s) | 91 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 344 |
| BseMII | CTCAG | 1 cut(s) | 60 |
| BseNI | ACTGG | 1 cut(s) | 458 |
| BseXI | GCAGC | 3 cut(s) | 170, 251, 335 |
| BseYI | CCCAGC | 1 cut(s) | 243 |
| BsgI | GTGCAG | 1 cut(s) | 169 |
| BshFI | GGCC | 2 cut(s) | 222, 248 |
| BsiSI | CCGG | 1 cut(s) | 275 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 344 |
| BsmAI | GTCTC | 1 cut(s) | 644 |
| BsnI | GGCC | 2 cut(s) | 222, 248 |
| Bsp143I | GATC | 1 cut(s) | 151 |
| BspACI | CCGC | 1 cut(s) | 52 |
| BspANI | GGCC | 2 cut(s) | 222, 248 |
| BspCNI | CTCAG | 1 cut(s) | 59 |
| BspLI | GGNNCC | 2 cut(s) | 64, 223 |
| BspMAI | CTGCAG | 1 cut(s) | 681 |
| BspMI | ACCTGC | 1 cut(s) | 670 |
| BsrI | ACTGG | 1 cut(s) | 458 |
| BssECI | CCNNGG | 1 cut(s) | 91 |
| BssMI | GATC | 1 cut(s) | 151 |
| Bst2UI | CCWGG | 1 cut(s) | 93 |
| Bst4CI | ACNGT | 2 cut(s) | 133, 565 |
| BstC8I | GCNNGC | 4 cut(s) | 52, 172, 334, 677 |
| BstDEI | CTNAG | 1 cut(s) | 46 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 342 |
| BstHHI | GCGC | 1 cut(s) | 332 |
| BstKTI | GATC | 1 cut(s) | 154 |
| BstMAI | GTCTC | 1 cut(s) | 644 |
| BstMBI | GATC | 1 cut(s) | 151 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 60, 114, 180, 228, 672 |
| BstNI | CCWGG | 1 cut(s) | 93 |
| BstNSI | RCATGY | 1 cut(s) | 336 |
| BstSCI | CCNGG | 1 cut(s) | 91 |
| BstSFI | CTRYAG | 1 cut(s) | 677 |
| BstV1I | GCAGC | 3 cut(s) | 170, 251, 335 |
| BsuRI | GGCC | 2 cut(s) | 222, 248 |
| BtsIMutI | CAGTG | 1 cut(s) | 152 |
| BveI | ACCTGC | 1 cut(s) | 670 |
| Cac8I | GCNNGC | 4 cut(s) | 52, 172, 334, 677 |
| CfoI | GCGC | 1 cut(s) | 332 |
| Cfr13I | GGNCC | 2 cut(s) | 221, 246 |
| Csp6I | GTAC | 1 cut(s) | 513 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 721, 756 |
| CviAII | CATG | 5 cut(s) | 35, 55, 302, 333, 720 |
| CviQI | GTAC | 1 cut(s) | 513 |
| DdeI | CTNAG | 1 cut(s) | 46 |
| DpnI | GATC | 1 cut(s) | 153 |
| DpnII | GATC | 1 cut(s) | 151 |
| Eco57I | CTGAAG | 1 cut(s) | 373 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 342 |
| EcoO109I | RGGNCCY | 1 cut(s) | 221 |
| EcoRII | CCWGG | 1 cut(s) | 91 |
| FaeI | CATG | 5 cut(s) | 38, 58, 305, 336, 723 |
| FatI | CATG | 5 cut(s) | 34, 54, 301, 332, 719 |
| FauI | CCCGC | 1 cut(s) | 59 |
| FbaI | TGATCA | 1 cut(s) | 151 |
| Fnu4HI | GCNGC | 3 cut(s) | 184, 265, 349 |
| Fsp4HI | GCNGC | 3 cut(s) | 184, 265, 349 |
| FspI | TGCGCA | 1 cut(s) | 331 |
| GlaI | GCGC | 1 cut(s) | 331 |
| GluI | GCNGC | 3 cut(s) | 184, 265, 349 |
| GsaI | CCCAGC | 1 cut(s) | 247 |
| HaeIII | GGCC | 2 cut(s) | 222, 248 |
| HapII | CCGG | 1 cut(s) | 275 |
| HhaI | GCGC | 1 cut(s) | 332 |
| Hin1II | CATG | 5 cut(s) | 38, 58, 305, 336, 723 |
| Hin6I | GCGC | 1 cut(s) | 330 |
| HinP1I | GCGC | 1 cut(s) | 330 |
| HincII | GTYRAC | 1 cut(s) | 84 |
| HindII | GTYRAC | 1 cut(s) | 84 |
| HinfI | GANTC | 2 cut(s) | 42, 407 |
| HpaI | GTTAAC | 1 cut(s) | 84 |
| HpaII | CCGG | 1 cut(s) | 275 |
| HphI | GGTGA | 2 cut(s) | 289, 667 |
| Hpy166II | GTNNAC | 2 cut(s) | 84, 515 |
| Hpy188III | TCNNGA | 3 cut(s) | 497, 699, 751 |
| Hpy8I | GTNNAC | 2 cut(s) | 84, 515 |
| HpyAV | CCTTC | 2 cut(s) | 53, 705 |
| HpyCH4III | ACNGT | 2 cut(s) | 133, 565 |
| HpyCH4IV | ACGT | 3 cut(s) | 80, 435, 464 |
| HpyCH4V | TGCA | 4 cut(s) | 186, 267, 351, 679 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 60, 114, 180, 228, 672 |
| HpyF3I | CTNAG | 1 cut(s) | 46 |
| HpySE526I | ACGT | 3 cut(s) | 80, 435, 464 |
| Hsp92II | CATG | 5 cut(s) | 38, 58, 305, 336, 723 |
| HspAI | GCGC | 1 cut(s) | 330 |
| Ksp22I | TGATCA | 1 cut(s) | 151 |
| KspAI | GTTAAC | 1 cut(s) | 84 |
| Kzo9I | GATC | 1 cut(s) | 151 |
| LmnI | GCTCC | 2 cut(s) | 68, 179 |
| Lsp1109I | GCAGC | 3 cut(s) | 170, 251, 335 |
| MaeII | ACGT | 3 cut(s) | 80, 435, 464 |
| MaeIII | GTNAC | 5 cut(s) | 277, 460, 559, 619, 655 |
| MalI | GATC | 1 cut(s) | 153 |
| MboI | GATC | 1 cut(s) | 151 |
| MboII | GAAGA | 2 cut(s) | 304, 354 |
| MluCI | AATT | 3 cut(s) | 111, 192, 352 |
| MnlI | CCTC | 5 cut(s) | 31, 220, 503, 536, 589 |
| MseI | TTAA | 6 cut(s) | 83, 195, 576, 590, 684, 713 |
| MspI | CCGG | 1 cut(s) | 275 |
| MspR9I | CCNGG | 1 cut(s) | 93 |
| MvaI | CCWGG | 1 cut(s) | 93 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 60, 114, 180, 228, 672 |
| NdeII | GATC | 1 cut(s) | 151 |
| NlaIII | CATG | 5 cut(s) | 38, 58, 305, 336, 723 |
| NlaIV | GGNNCC | 2 cut(s) | 64, 223 |
| NmuCI | GTSAC | 3 cut(s) | 277, 559, 655 |
| NsbI | TGCGCA | 1 cut(s) | 331 |
| NspI | RCATGY | 1 cut(s) | 336 |
| PaeI | GCATGC | 1 cut(s) | 336 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 235 |
| PfeI | GAWTC | 2 cut(s) | 42, 407 |
| PkrI | GCNGC | 3 cut(s) | 185, 266, 350 |
| PshBI | ATTAAT | 1 cut(s) | 195 |
| Psp1406I | AACGTT | 2 cut(s) | 80, 435 |
| Psp6I | CCWGG | 1 cut(s) | 91 |
| PspFI | CCCAGC | 1 cut(s) | 243 |
| PspGI | CCWGG | 1 cut(s) | 91 |
| PspN4I | GGNNCC | 2 cut(s) | 64, 223 |
| PspPI | GGNCC | 2 cut(s) | 221, 246 |
| PstI | CTGCAG | 1 cut(s) | 681 |
| RsaI | GTAC | 1 cut(s) | 514 |
| RsaNI | GTAC | 1 cut(s) | 513 |
| SaqAI | TTAA | 6 cut(s) | 83, 195, 576, 590, 684, 713 |
| SatI | GCNGC | 3 cut(s) | 184, 265, 349 |
| Sau3AI | GATC | 1 cut(s) | 151 |
| Sau96I | GGNCC | 2 cut(s) | 221, 246 |
| SbfI | CCTGCAGG | 1 cut(s) | 681 |
| ScrFI | CCNGG | 1 cut(s) | 93 |
| SdaI | CCTGCAGG | 1 cut(s) | 681 |
| SfcI | CTRYAG | 1 cut(s) | 677 |
| SphI | GCATGC | 1 cut(s) | 336 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 681 |
| Sse9I | AATT | 3 cut(s) | 111, 192, 352 |
| SsiI | CCGC | 1 cut(s) | 52 |
| SspI | AATATT | 1 cut(s) | 70 |
| StyD4I | CCNGG | 1 cut(s) | 91 |
| TaaI | ACNGT | 2 cut(s) | 133, 565 |
| TaiI | ACGT | 3 cut(s) | 83, 438, 467 |
| TaqI | TCGA | 1 cut(s) | 496 |
| TasI | AATT | 3 cut(s) | 111, 192, 352 |
| TfiI | GAWTC | 2 cut(s) | 42, 407 |
| Tru1I | TTAA | 6 cut(s) | 83, 195, 576, 590, 684, 713 |
| Tru9I | TTAA | 6 cut(s) | 83, 195, 576, 590, 684, 713 |
| TscAI | CASTG | 1 cut(s) | 152 |
| TseFI | GTSAC | 3 cut(s) | 277, 559, 655 |
| TseI | GCWGC | 3 cut(s) | 183, 264, 348 |
| Tsp45I | GTSAC | 3 cut(s) | 277, 559, 655 |
| TspDTI | ATGAA | 3 cut(s) | 17, 312, 318 |
| TspRI | CASTG | 1 cut(s) | 152 |
| VspI | ATTAAT | 1 cut(s) | 195 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 342 |
| XceI | RCATGY | 1 cut(s) | 336 |