E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 793 |
| Acc65I | GGTACC | 1 cut(s) | 638 |
| AccB1I | GGYRCC | 1 cut(s) | 638 |
| AccIII | TCCGGA | 1 cut(s) | 7 |
| AciI | CCGC | 1 cut(s) | 273 |
| AclWI | GGATC | 2 cut(s) | 12, 126 |
| AcsI | RAATTY | 1 cut(s) | 767 |
| AcuI | CTGAAG | 4 cut(s) | 117, 499, 614, 687 |
| AfaI | GTAC | 7 cut(s) | 39, 60, 361, 506, 640, 664, 790 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 470 |
| AflIII | ACRYGT | 1 cut(s) | 437 |
| AgsI | TTSAA | 2 cut(s) | 704, 856 |
| AjiI | CACGTC | 1 cut(s) | 438 |
| AjnI | CCWGG | 1 cut(s) | 178 |
| AluBI | AGCT | 4 cut(s) | 98, 137, 257, 711 |
| AluI | AGCT | 4 cut(s) | 98, 137, 257, 711 |
| Alw26I | GTCTC | 1 cut(s) | 102 |
| AlwI | GGATC | 2 cut(s) | 12, 126 |
| Aor13HI | TCCGGA | 1 cut(s) | 7 |
| AoxI | GGCC | 1 cut(s) | 811 |
| ApeKI | GCWGC | 2 cut(s) | 390, 474 |
| ApoI | RAATTY | 1 cut(s) | 767 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 722 |
| Asp718I | GGTACC | 1 cut(s) | 638 |
| AspLEI | GCGC | 1 cut(s) | 451 |
| AspS9I | GGNCC | 1 cut(s) | 812 |
| AsuC2I | CCSGG | 1 cut(s) | 332 |
| AsuNHI | GCTAGC | 1 cut(s) | 137 |
| BamHI | GGATCC | 1 cut(s) | 4 |
| BanI | GGYRCC | 1 cut(s) | 638 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 497, 529 |
| BbsI | GAAGAC | 3 cut(s) | 93, 220, 440 |
| BbvCI | CCTCAGC | 1 cut(s) | 78 |
| BbvI | GCAGC | 2 cut(s) | 377, 461 |
| BccI | CCATC | 2 cut(s) | 563, 803 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 104, 138 |
| BciT130I | CCWGG | 1 cut(s) | 180 |
| BclI | TGATCA | 1 cut(s) | 238 |
| BcnI | CCSGG | 1 cut(s) | 332 |
| BcoDI | GTCTC | 1 cut(s) | 102 |
| BfaI | CTAG | 3 cut(s) | 138, 708, 861 |
| BfmI | CTRYAG | 1 cut(s) | 800 |
| BfuAI | ACCTGC | 1 cut(s) | 793 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 315 |
| BisI | GCNGC | 3 cut(s) | 274, 391, 475 |
| BlsI | GCNGC | 3 cut(s) | 275, 392, 476 |
| BmcAI | AGTACT | 1 cut(s) | 790 |
| Bme1390I | CCNGG | 2 cut(s) | 180, 332 |
| BmgBI | CACGTC | 1 cut(s) | 438 |
| BmgT120I | GGNCC | 1 cut(s) | 812 |
| BmiI | GGNNCC | 2 cut(s) | 6, 640 |
| BmrFI | CCNGG | 2 cut(s) | 180, 332 |
| BmsI | GCATC | 1 cut(s) | 494 |
| BmtI | GCTAGC | 1 cut(s) | 141 |
| BpiI | GAAGAC | 3 cut(s) | 93, 220, 440 |
| BpmI | CTGGAG | 1 cut(s) | 126 |
| Bpu10I | CCTNAGC | 1 cut(s) | 78 |
| BpuMI | CCSGG | 1 cut(s) | 332 |
| BsaJI | CCNNGG | 1 cut(s) | 178 |
| BsaWI | WCCGGW | 1 cut(s) | 7 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 470 |
| Bse1I | ACTGG | 2 cut(s) | 232, 401 |
| Bse3DI | GCAATG | 1 cut(s) | 526 |
| BseAI | TCCGGA | 1 cut(s) | 7 |
| BseBI | CCWGG | 1 cut(s) | 180 |
| BseDI | CCNNGG | 1 cut(s) | 178 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 470 |
| BseMI | GCAATG | 1 cut(s) | 526 |
| BseMII | CTCAG | 2 cut(s) | 27, 69 |
| BseNI | ACTGG | 2 cut(s) | 232, 401 |
| BseXI | GCAGC | 2 cut(s) | 377, 461 |
| Bsh1285I | CGRYCG | 1 cut(s) | 339 |
| BshFI | GGCC | 1 cut(s) | 813 |
| BshNI | GGYRCC | 1 cut(s) | 638 |
| BsiEI | CGRYCG | 1 cut(s) | 339 |
| BsiSI | CCGG | 2 cut(s) | 8, 331 |
| BsiWI | CGTACG | 1 cut(s) | 58 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 470 |
| BsmAI | GTCTC | 1 cut(s) | 102 |
| BsnI | GGCC | 1 cut(s) | 813 |
| Bsp13I | TCCGGA | 1 cut(s) | 7 |
| Bsp143I | GATC | 4 cut(s) | 4, 118, 238, 336 |
| BspACI | CCGC | 1 cut(s) | 273 |
| BspANI | GGCC | 1 cut(s) | 813 |
| BspCNI | CTCAG | 2 cut(s) | 26, 70 |
| BspEI | TCCGGA | 1 cut(s) | 7 |
| BspHI | TCATGA | 1 cut(s) | 714 |
| BspLI | GGNNCC | 2 cut(s) | 6, 640 |
| BspMAI | CTGCAG | 1 cut(s) | 804 |
| BspMI | ACCTGC | 1 cut(s) | 793 |
| BspOI | GCTAGC | 1 cut(s) | 141 |
| BspPI | GGATC | 2 cut(s) | 12, 126 |
| BspT107I | GGYRCC | 1 cut(s) | 638 |
| BsrDI | GCAATG | 1 cut(s) | 526 |
| BsrI | ACTGG | 2 cut(s) | 232, 401 |
| BssECI | CCNNGG | 1 cut(s) | 178 |
| BssMI | GATC | 4 cut(s) | 4, 118, 238, 336 |
| Bst2UI | CCWGG | 1 cut(s) | 180 |
| Bst4CI | ACNGT | 3 cut(s) | 220, 691, 894 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 491 |
| BstC8I | GCNNGC | 4 cut(s) | 139, 259, 307, 800 |
| BstDEI | CTNAG | 3 cut(s) | 13, 78, 873 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 468 |
| BstHHI | GCGC | 1 cut(s) | 451 |
| BstKTI | GATC | 4 cut(s) | 7, 121, 241, 339 |
| BstMAI | GTCTC | 1 cut(s) | 102 |
| BstMBI | GATC | 4 cut(s) | 4, 118, 238, 336 |
| BstMCI | CGRYCG | 1 cut(s) | 339 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 147, 201, 315, 378, 387, 491 |
| BstNI | CCWGG | 1 cut(s) | 180 |
| BstSCI | CCNGG | 2 cut(s) | 178, 330 |
| BstSFI | CTRYAG | 1 cut(s) | 800 |
| BstV1I | GCAGC | 2 cut(s) | 377, 461 |
| BstV2I | GAAGAC | 3 cut(s) | 93, 220, 440 |
| BstX2I | RGATCY | 1 cut(s) | 4 |
| BstYI | RGATCY | 1 cut(s) | 4 |
| BsuRI | GGCC | 1 cut(s) | 813 |
| BtrI | CACGTC | 1 cut(s) | 438 |
| BtsIMutI | CAGTG | 1 cut(s) | 239 |
| BveI | ACCTGC | 1 cut(s) | 793 |
| Cac8I | GCNNGC | 4 cut(s) | 139, 259, 307, 800 |
| CciI | TCATGA | 1 cut(s) | 714 |
| CfoI | GCGC | 1 cut(s) | 451 |
| Cfr13I | GGNCC | 1 cut(s) | 812 |
| Csp6I | GTAC | 7 cut(s) | 38, 59, 360, 505, 639, 663, 789 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 844, 879 |
| CviAII | CATG | 5 cut(s) | 122, 369, 428, 459, 715 |
| CviQI | GTAC | 7 cut(s) | 38, 59, 360, 505, 639, 663, 789 |
| DdeI | CTNAG | 3 cut(s) | 13, 78, 873 |
| DpnI | GATC | 4 cut(s) | 6, 120, 240, 338 |
| DpnII | GATC | 4 cut(s) | 4, 118, 238, 336 |
| Eco57I | CTGAAG | 4 cut(s) | 117, 499, 614, 687 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 468 |
| EcoRII | CCWGG | 1 cut(s) | 178 |
| FaeI | CATG | 5 cut(s) | 125, 372, 431, 462, 718 |
| FatI | CATG | 5 cut(s) | 121, 368, 427, 458, 714 |
| FbaI | TGATCA | 1 cut(s) | 238 |
| Fnu4HI | GCNGC | 3 cut(s) | 274, 391, 475 |
| Fsp4HI | GCNGC | 3 cut(s) | 274, 391, 475 |
| FspBI | CTAG | 3 cut(s) | 138, 708, 861 |
| GlaI | GCGC | 1 cut(s) | 450 |
| GluI | GCNGC | 3 cut(s) | 274, 391, 475 |
| GsuI | CTGGAG | 1 cut(s) | 126 |
| HaeIII | GGCC | 1 cut(s) | 813 |
| HapII | CCGG | 2 cut(s) | 8, 331 |
| HhaI | GCGC | 1 cut(s) | 451 |
| Hin1II | CATG | 5 cut(s) | 125, 372, 431, 462, 718 |
| Hin6I | GCGC | 1 cut(s) | 449 |
| HinP1I | GCGC | 1 cut(s) | 449 |
| HinfI | GANTC | 4 cut(s) | 11, 17, 353, 533 |
| HpaII | CCGG | 2 cut(s) | 8, 331 |
| Hpy166II | GTNNAC | 1 cut(s) | 209 |
| Hpy188I | TCNGA | 2 cut(s) | 16, 25 |
| Hpy188III | TCNNGA | 4 cut(s) | 8, 340, 623, 715 |
| Hpy8I | GTNNAC | 1 cut(s) | 209 |
| HpyAV | CCTTC | 2 cut(s) | 140, 825 |
| HpyCH4III | ACNGT | 3 cut(s) | 220, 691, 894 |
| HpyCH4IV | ACGT | 1 cut(s) | 437 |
| HpyCH4V | TGCA | 5 cut(s) | 393, 477, 485, 802, 897 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 147, 201, 315, 378, 387, 491 |
| HpyF3I | CTNAG | 3 cut(s) | 13, 78, 873 |
| HpySE526I | ACGT | 1 cut(s) | 437 |
| Hsp92II | CATG | 5 cut(s) | 125, 372, 431, 462, 718 |
| HspAI | GCGC | 1 cut(s) | 449 |
| Kpn2I | TCCGGA | 1 cut(s) | 7 |
| KpnI | GGTACC | 1 cut(s) | 642 |
| Ksp22I | TGATCA | 1 cut(s) | 238 |
| Kzo9I | GATC | 4 cut(s) | 4, 118, 238, 336 |
| LmnI | GCTCC | 1 cut(s) | 266 |
| Lsp1109I | GCAGC | 2 cut(s) | 377, 461 |
| LweI | GCATC | 1 cut(s) | 494 |
| MaeI | CTAG | 3 cut(s) | 138, 708, 861 |
| MaeII | ACGT | 1 cut(s) | 437 |
| MaeIII | GTNAC | 3 cut(s) | 685, 742, 778 |
| MalI | GATC | 4 cut(s) | 6, 120, 240, 338 |
| MboI | GATC | 4 cut(s) | 4, 118, 238, 336 |
| MfeI | CAATTG | 1 cut(s) | 72 |
| MflI | RGATCY | 1 cut(s) | 4 |
| MluCI | AATT | 6 cut(s) | 72, 198, 279, 478, 585, 767 |
| MlyI | GAGTC | 1 cut(s) | 5 |
| MmeI | TCCRAC | 1 cut(s) | 845 |
| MnlI | CCTC | 3 cut(s) | 73, 109, 745 |
| MroI | TCCGGA | 1 cut(s) | 7 |
| MroXI | GAANNNNTTC | 1 cut(s) | 722 |
| MseI | TTAA | 2 cut(s) | 282, 836 |
| MspI | CCGG | 2 cut(s) | 8, 331 |
| MspR9I | CCNGG | 2 cut(s) | 180, 332 |
| MunI | CAATTG | 1 cut(s) | 72 |
| MvaI | CCWGG | 1 cut(s) | 180 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 147, 201, 315, 378, 387, 491 |
| NciI | CCSGG | 1 cut(s) | 332 |
| NdeII | GATC | 4 cut(s) | 4, 118, 238, 336 |
| NheI | GCTAGC | 1 cut(s) | 137 |
| NlaIII | CATG | 5 cut(s) | 125, 372, 431, 462, 718 |
| NlaIV | GGNNCC | 2 cut(s) | 6, 640 |
| NmuCI | GTSAC | 2 cut(s) | 685, 778 |
| PagI | TCATGA | 1 cut(s) | 714 |
| PdmI | GAANNNNTTC | 1 cut(s) | 722 |
| PfeI | GAWTC | 3 cut(s) | 17, 353, 533 |
| Pfl23II | CGTACG | 1 cut(s) | 58 |
| PkrI | GCNGC | 3 cut(s) | 275, 392, 476 |
| Ple19I | CGATCG | 1 cut(s) | 339 |
| PleI | GAGTC | 1 cut(s) | 5 |
| PpsI | GAGTC | 1 cut(s) | 5 |
| Psp6I | CCWGG | 1 cut(s) | 178 |
| PspGI | CCWGG | 1 cut(s) | 178 |
| PspLI | CGTACG | 1 cut(s) | 58 |
| PspN4I | GGNNCC | 2 cut(s) | 6, 640 |
| PspPI | GGNCC | 1 cut(s) | 812 |
| PstI | CTGCAG | 1 cut(s) | 804 |
| PsuI | RGATCY | 1 cut(s) | 4 |
| PvuI | CGATCG | 1 cut(s) | 339 |
| RsaI | GTAC | 7 cut(s) | 39, 60, 361, 506, 640, 664, 790 |
| RsaNI | GTAC | 7 cut(s) | 38, 59, 360, 505, 639, 663, 789 |
| SaqAI | TTAA | 2 cut(s) | 282, 836 |
| SatI | GCNGC | 3 cut(s) | 274, 391, 475 |
| Sau3AI | GATC | 4 cut(s) | 4, 118, 238, 336 |
| Sau96I | GGNCC | 1 cut(s) | 812 |
| SbfI | CCTGCAGG | 1 cut(s) | 804 |
| ScaI | AGTACT | 1 cut(s) | 790 |
| SchI | GAGTC | 1 cut(s) | 5 |
| ScrFI | CCNGG | 2 cut(s) | 180, 332 |
| SdaI | CCTGCAGG | 1 cut(s) | 804 |
| SetI | ASST | 8 cut(s) | 100, 139, 259, 440, 644, 709, 713, 807 |
| SfaNI | GCATC | 1 cut(s) | 494 |
| SfcI | CTRYAG | 1 cut(s) | 800 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 804 |
| Sse9I | AATT | 6 cut(s) | 72, 198, 279, 478, 585, 767 |
| SsiI | CCGC | 1 cut(s) | 273 |
| SspI | AATATT | 2 cut(s) | 157, 397 |
| SspMI | CTAG | 3 cut(s) | 138, 708, 861 |
| StyD4I | CCNGG | 2 cut(s) | 178, 330 |
| TaaI | ACNGT | 3 cut(s) | 220, 691, 894 |
| TaiI | ACGT | 1 cut(s) | 440 |
| TaqI | TCGA | 3 cut(s) | 128, 339, 622 |
| TaqII | GACCGA | 1 cut(s) | 762 |
| TasI | AATT | 6 cut(s) | 72, 198, 279, 478, 585, 767 |
| TatI | WGTACW | 2 cut(s) | 359, 788 |
| TauI | GCSGC | 1 cut(s) | 276 |
| TfiI | GAWTC | 3 cut(s) | 17, 353, 533 |
| Tru1I | TTAA | 2 cut(s) | 282, 836 |
| Tru9I | TTAA | 2 cut(s) | 282, 836 |
| TscAI | CASTG | 1 cut(s) | 239 |
| TseFI | GTSAC | 2 cut(s) | 685, 778 |
| TseI | GCWGC | 2 cut(s) | 390, 474 |
| Tsp45I | GTSAC | 2 cut(s) | 685, 778 |
| TspDTI | ATGAA | 6 cut(s) | 438, 515, 637, 703, 715, 731 |
| TspRI | CASTG | 1 cut(s) | 239 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 468 |
| XapI | RAATTY | 1 cut(s) | 767 |
| XmnI | GAANNNNTTC | 1 cut(s) | 722 |
| XspI | CTAG | 3 cut(s) | 138, 708, 861 |
| ZrmI | AGTACT | 1 cut(s) | 790 |