RchiOBHm_Chr6g0253941

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000395
Physical Location & Seq
Forward (+)
9059355 .. 9059810
456 bp
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UTR
Exon/CDS
Intron
PRQ22774

Sequence Viewer

Length: 456 bp
ATGAATGTAGACGAAGAACCCAATAATCGACCGTCTAATGCGGAAGACCAAGAGGGTCGTAGTTCGACTTCAGCTAGCAGGGAAGGCTCTAATATTATGATAACTTTAACTGACCCAGGACTGTTAGATTGCCCAATTTGCTGTGAACCCTTGACTATCCCTGTCTTCCAGTGTGATCAGAATGGGCATACAGGTGGTTCCTTGTGCTGCACCAAAATTAGTAACAAATGCCCCTCTTGTTCCTGCCCCATTGGCTCTAATCGTTGCCGAGCCATCGAGAAAGTTGTGGAATCAAGTACATGTCCTTGCCGAAATATCAAGTATGGCTGCAATATTCCAGTGACTTACAACAAGAAGAATGAACATGAAAAGACGTGTATGTTCACCTTGTTCATGCCTTTATTTAGGTTGCAAGTTTGTTTCTTCGGCCAAGGAGTTATATCAACACTTCAGTAA

Protein Analysis

151

Amino Acids

16.6

Weight (kDa)

6.1

Isoelectric Point (pI)

61.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sina_ZnF PF21361 94 - 132 4.5e-06 Sina, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000395)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66610 AT1G66610 AT1G66620 AT1G66630 AT1G66650 AT1G66660 AT1G66660 AT5G37870 AT5G37890 AT5G37900 AT5G37910 AT5G37930 AT5G62800 AT5G62800
fragaria_vesca FvH4_1g29100 FvH4_1g29100 FvH4_1g29100 FvH4_1g29100 FvH4_1g29100 FvH4_1g29100 FvH4_1g29100 FvH4_2g03850 FvH4_2g04470 FvH4_2g04471
malus_domestica MD09G1260200.v1.1 MD09G1260400.v1.1 MD10G1106400.v1.1
prunus_persica Prupe.3G152000_v2.0.a1 Prupe.3G152100_v2.0.a1 Prupe.3G152100_v2.0.a1 Prupe.3G152100_v2.0.a1 Prupe.8G145400_v2.0.a1 Prupe.8G145500_v2.0.a1
pyrus_communis pycom09g17480 pycom09g17490 pycom10g09160
rosa_chinensis RchiOBHm_Chr2g0111691 RchiOBHm_Chr3g0448681 RchiOBHm_Chr3g0493571 RchiOBHm_Chr6g0252861 RchiOBHm_Chr6g0252871 RchiOBHm_Chr6g0253841 RchiOBHm_Chr6g0253921 RchiOBHm_Chr6g0253941
rosa_laevigata RLG00000007595 RLG00000014953 RLG00000014955 RLG00000014957 RLG00000014958 RLG00000015058 RLG00000017917 RLG00000022703
rosa_multiflora Rmu_sc0000829.1_g000012 Rmu_sc0000829.1_g000015 Rmu_sc0000829.1_g000016 Rmu_sc0000927.1_g000006 Rmu_sc0001716.1_g000004 Rmu_sc0003921.1_g000007
rosa_roxburghii Rroxscaffold_4G00307570 Rroxscaffold_6G00391470 Rroxscaffold_6G00426970 Rroxscaffold_7G00211390 Rroxscaffold_7G00211420 Rroxscaffold_7G00211430 Rroxscaffold_7G00212350 Rroxscaffold_7G00212370
rosa_rugosa Rorug02G0175400 Rorug02G0615300 Rorug03G0262600 Rorug03G0262700 Rorug05G0539200 Rorug05G0539400 Rorug05G0548300 Rorug05G0548400 Rorug05G0548500 Rorug05G0548600
rosa_samantha Rh2BG240600 Rh3AG014100 Rh3AG311400 Rh3BG013900 Rh3BG347300 Rh3CG345400 Rh3DG347600 Rh6AG055200 Rh6AG063900 Rh6AG064600 Rh6AG064800 Rh6BG049400 Rh6BG049500 Rh6BG057700 Rh6BG058000 Rh6CG048200 Rh6CG048300 Rh6CG057600 Rh6CG057900 Rh6DG043800 Rh6DG043900
rosa_wichuraiana Rw2G017640 Rw6G005000 Rw6G005670 Rw6G005710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 9
AciI CCGC 1 cut(s) 41
AcoI YGGCCR 1 cut(s) 427
AcuI CTGAAG 2 cut(s) 54, 434
AfaI GTAC 1 cut(s) 298
AflIII ACRYGT 2 cut(s) 299, 374
AjiI CACGTC 1 cut(s) 375
AjnI CCWGG 1 cut(s) 115
AluBI AGCT 1 cut(s) 74
AluI AGCT 1 cut(s) 74
AoxI GGCC 1 cut(s) 427
ApeKI GCWGC 2 cut(s) 207, 327
AsuHPI GGTGA 1 cut(s) 376
AsuNHI GCTAGC 1 cut(s) 74
BarI GAAGNNNNNNTAC 2 cut(s) 52, 84
BbsI GAAGAC 2 cut(s) 51, 157
BbvI GCAGC 2 cut(s) 194, 314
BccI CCATC 1 cut(s) 281
BciT130I CCWGG 1 cut(s) 117
BclI TGATCA 1 cut(s) 175
BfaI CTAG 1 cut(s) 75
BglI GCCNNNNNGGC 1 cut(s) 252
BisI GCNGC 2 cut(s) 208, 328
BlsI GCNGC 2 cut(s) 209, 329
Bme1390I CCNGG 1 cut(s) 117
BmgBI CACGTC 1 cut(s) 375
BmiI GGNNCC 1 cut(s) 199
BmrFI CCNGG 1 cut(s) 117
BmtI GCTAGC 1 cut(s) 78
BpiI GAAGAC 2 cut(s) 51, 157
BsaJI CCNNGG 2 cut(s) 115, 430
Bse1I ACTGG 2 cut(s) 169, 338
BseBI CCWGG 1 cut(s) 117
BseDI CCNNGG 2 cut(s) 115, 430
BseNI ACTGG 2 cut(s) 169, 338
BseXI GCAGC 2 cut(s) 194, 314
BsgI GTGCAG 1 cut(s) 193
Bsh1285I CGRYCG 1 cut(s) 32
BshFI GGCC 1 cut(s) 429
BsiEI CGRYCG 1 cut(s) 32
BsnI GGCC 1 cut(s) 429
Bsp143I GATC 1 cut(s) 175
BspACI CCGC 1 cut(s) 41
BspANI GGCC 1 cut(s) 429
BspLI GGNNCC 1 cut(s) 199
BspOI GCTAGC 1 cut(s) 78
BsrI ACTGG 2 cut(s) 169, 338
BssECI CCNNGG 2 cut(s) 115, 430
BssMI GATC 1 cut(s) 175
BssT1I CCWWGG 1 cut(s) 430
Bst2UI CCWGG 1 cut(s) 117
Bst4CI ACNGT 2 cut(s) 33, 123
BstC8I GCNNGC 1 cut(s) 76
BstKTI GATC 1 cut(s) 178
BstMBI GATC 1 cut(s) 175
BstMCI CGRYCG 1 cut(s) 32
BstMWI GCNNNNNNNGC 3 cut(s) 84, 138, 252
BstNI CCWGG 1 cut(s) 117
BstNSI RCATGY 1 cut(s) 303
BstSCI CCNGG 1 cut(s) 115
BstV1I GCAGC 2 cut(s) 194, 314
BstV2I GAAGAC 2 cut(s) 51, 157
BsuRI GGCC 1 cut(s) 429
BtrI CACGTC 1 cut(s) 375
BtsIMutI CAGTG 2 cut(s) 176, 345
Cac8I GCNNGC 1 cut(s) 76
Csp6I GTAC 1 cut(s) 297
CviAII CATG 3 cut(s) 300, 365, 394
CviJI RGCY 6 cut(s) 74, 87, 255, 272, 327, 429
CviKI_1 RGCY 6 cut(s) 74, 87, 255, 272, 327, 429
CviQI GTAC 1 cut(s) 297
DpnI GATC 1 cut(s) 177
DpnII GATC 1 cut(s) 175
EaeI YGGCCR 1 cut(s) 427
Eco130I CCWWGG 1 cut(s) 430
Eco57I CTGAAG 2 cut(s) 54, 434
EcoRII CCWGG 1 cut(s) 115
EcoT14I CCWWGG 1 cut(s) 430
ErhI CCWWGG 1 cut(s) 430
FaeI CATG 3 cut(s) 303, 368, 397
FaiI YATR 8 cut(s) 98, 189, 301, 324, 366, 380, 395, 440
FatI CATG 3 cut(s) 299, 364, 393
FbaI TGATCA 1 cut(s) 175
FblI GTMKAC 1 cut(s) 9
Fnu4HI GCNGC 2 cut(s) 208, 328
Fsp4HI GCNGC 2 cut(s) 208, 328
FspBI CTAG 1 cut(s) 75
GluI GCNGC 2 cut(s) 208, 328
HaeIII GGCC 1 cut(s) 429
Hin1II CATG 3 cut(s) 303, 368, 397
HinfI GANTC 1 cut(s) 290
HphI GGTGA 1 cut(s) 376
Hpy166II GTNNAC 3 cut(s) 10, 146, 384
Hpy188I TCNGA 1 cut(s) 180
Hpy188III TCNNGA 1 cut(s) 277
Hpy8I GTNNAC 3 cut(s) 10, 146, 384
HpyAV CCTTC 1 cut(s) 77
HpyCH4III ACNGT 2 cut(s) 33, 123
HpyCH4IV ACGT 1 cut(s) 374
HpyCH4V TGCA 3 cut(s) 210, 330, 412
HpyF10VI GCNNNNNNNGC 3 cut(s) 84, 138, 252
HpySE526I ACGT 1 cut(s) 374
Hsp92II CATG 3 cut(s) 303, 368, 397
Ksp22I TGATCA 1 cut(s) 175
Kzo9I GATC 1 cut(s) 175
LpnPI CCDG 8 cut(s) 64, 102, 129, 174, 177, 182, 256, 351
Lsp1109I GCAGC 2 cut(s) 194, 314
MaeI CTAG 1 cut(s) 75
MaeII ACGT 1 cut(s) 374
MaeIII GTNAC 2 cut(s) 221, 340
MalI GATC 1 cut(s) 177
MboI GATC 1 cut(s) 175
MboII GAAGA 5 cut(s) 26, 56, 157, 367, 415
MluCI AATT 2 cut(s) 135, 216
MnlI CCTC 2 cut(s) 46, 244
MseI TTAA 1 cut(s) 107
MslI CAYNNNNRTG 1 cut(s) 192
MspR9I CCNGG 1 cut(s) 117
MvaI CCWGG 1 cut(s) 117
MwoI GCNNNNNNNGC 3 cut(s) 84, 138, 252
NdeII GATC 1 cut(s) 175
NheI GCTAGC 1 cut(s) 74
NlaIII CATG 3 cut(s) 303, 368, 397
NlaIV GGNNCC 1 cut(s) 199
NmeAIII GCCGAG 1 cut(s) 293
NmuCI GTSAC 1 cut(s) 340
NspI RCATGY 1 cut(s) 303
PciI ACATGT 1 cut(s) 299
PfeI GAWTC 1 cut(s) 290
PkrI GCNGC 2 cut(s) 209, 329
PscI ACATGT 1 cut(s) 299
Psp6I CCWGG 1 cut(s) 115
PspGI CCWGG 1 cut(s) 115
PspN4I GGNNCC 1 cut(s) 199
RsaI GTAC 1 cut(s) 298
RsaNI GTAC 1 cut(s) 297
RseI CAYNNNNRTG 1 cut(s) 192
SaqAI TTAA 1 cut(s) 107
SatI GCNGC 2 cut(s) 208, 328
Sau3AI GATC 1 cut(s) 175
ScrFI CCNGG 1 cut(s) 117
SetI ASST 5 cut(s) 76, 196, 377, 389, 410
SmiMI CAYNNNNRTG 1 cut(s) 192
Sse9I AATT 2 cut(s) 135, 216
SsiI CCGC 1 cut(s) 41
SspI AATATT 2 cut(s) 94, 334
SspMI CTAG 1 cut(s) 75
StyD4I CCNGG 1 cut(s) 115
StyI CCWWGG 1 cut(s) 430
TaaI ACNGT 2 cut(s) 33, 123
TaiI ACGT 1 cut(s) 377
TaqI TCGA 3 cut(s) 28, 65, 276
TasI AATT 2 cut(s) 135, 216
TatI WGTACW 1 cut(s) 296
TfiI GAWTC 1 cut(s) 290
Tru1I TTAA 1 cut(s) 107
Tru9I TTAA 1 cut(s) 107
TscAI CASTG 2 cut(s) 176, 345
TseFI GTSAC 1 cut(s) 340
TseI GCWGC 2 cut(s) 207, 327
Tsp45I GTSAC 1 cut(s) 340
TspDTI ATGAA 4 cut(s) 17, 375, 381, 382
TspRI CASTG 2 cut(s) 176, 345
XceI RCATGY 1 cut(s) 303
XmiI GTMKAC 1 cut(s) 9
XspI CTAG 1 cut(s) 75
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.