E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1229 |
| Acc16I | TGCGCA | 1 cut(s) | 933 |
| AccB1I | GGYRCC | 1 cut(s) | 201 |
| AccII | CGCG | 2 cut(s) | 370, 1190 |
| AccIII | TCCGGA | 1 cut(s) | 419 |
| AciI | CCGC | 6 cut(s) | 251, 365, 428, 508, 919, 1190 |
| AclWI | GGATC | 3 cut(s) | 308, 496, 560 |
| AcoI | YGGCCR | 1 cut(s) | 136 |
| AcsI | RAATTY | 1 cut(s) | 578 |
| AcuI | CTGAAG | 1 cut(s) | 960 |
| AfaI | GTAC | 4 cut(s) | 185, 416, 594, 949 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 134, 217, 257, 1034, 1088, 1244 |
| AgsI | TTSAA | 8 cut(s) | 154, 724, 766, 808, 990, 1013, 1034, 1107 |
| AjiI | CACGTC | 1 cut(s) | 523 |
| AjnI | CCWGG | 1 cut(s) | 616 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1021, 1053 |
| AluBI | AGCT | 3 cut(s) | 484, 611, 1170 |
| AluI | AGCT | 3 cut(s) | 484, 611, 1170 |
| Alw21I | GWGCWC | 1 cut(s) | 279 |
| Alw26I | GTCTC | 2 cut(s) | 64, 520 |
| AlwI | GGATC | 3 cut(s) | 308, 496, 560 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 611 |
| Ama87I | CYCGRG | 1 cut(s) | 1177 |
| Aor13HI | TCCGGA | 1 cut(s) | 419 |
| AoxI | GGCC | 2 cut(s) | 136, 1025 |
| ApeKI | GCWGC | 1 cut(s) | 1195 |
| ApoI | RAATTY | 1 cut(s) | 578 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 275, 307 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 95 |
| AspLEI | GCGC | 2 cut(s) | 370, 934 |
| AspS9I | GGNCC | 2 cut(s) | 614, 1025 |
| AsuC2I | CCSGG | 2 cut(s) | 1178, 1179 |
| AsuHPI | GGTGA | 2 cut(s) | 854, 1156 |
| AvaI | CYCGRG | 1 cut(s) | 1177 |
| AvaII | GGWCC | 1 cut(s) | 614 |
| BaeGI | GKGCMC | 1 cut(s) | 1156 |
| BanI | GGYRCC | 1 cut(s) | 201 |
| BbsI | GAAGAC | 1 cut(s) | 985 |
| Bbv12I | GWGCWC | 1 cut(s) | 279 |
| BbvI | GCAGC | 1 cut(s) | 1182 |
| BccI | CCATC | 4 cut(s) | 101, 349, 625, 708 |
| BciT130I | CCWGG | 1 cut(s) | 618 |
| BcnI | CCSGG | 2 cut(s) | 1178, 1179 |
| BcoDI | GTCTC | 2 cut(s) | 64, 520 |
| BfmI | CTRYAG | 4 cut(s) | 196, 305, 318, 796 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1004 |
| BisI | GCNGC | 3 cut(s) | 366, 919, 1196 |
| BlsI | GCNGC | 3 cut(s) | 367, 920, 1197 |
| BmcAI | AGTACT | 2 cut(s) | 416, 949 |
| Bme1390I | CCNGG | 3 cut(s) | 618, 1178, 1179 |
| Bme18I | GGWCC | 1 cut(s) | 614 |
| BmeT110I | CYCGRG | 1 cut(s) | 1177 |
| BmgBI | CACGTC | 1 cut(s) | 523 |
| BmgT120I | GGNCC | 2 cut(s) | 614, 1025 |
| BmiI | GGNNCC | 3 cut(s) | 203, 339, 1026 |
| BmrFI | CCNGG | 3 cut(s) | 618, 1178, 1179 |
| BmsI | GCATC | 1 cut(s) | 385 |
| BpiI | GAAGAC | 1 cut(s) | 985 |
| BpmI | CTGGAG | 2 cut(s) | 84, 733 |
| Bpu10I | CCTNAGC | 1 cut(s) | 112 |
| BpuEI | CTTGAG | 2 cut(s) | 365, 395 |
| BpuMI | CCSGG | 2 cut(s) | 1178, 1179 |
| BsaBI | GATNNNNATC | 1 cut(s) | 446 |
| BsaJI | CCNNGG | 4 cut(s) | 133, 1111, 1177, 1178 |
| BsaWI | WCCGGW | 1 cut(s) | 419 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 541, 571 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 134, 217, 257, 1034, 1088, 1244 |
| Bse1I | ACTGG | 3 cut(s) | 128, 817, 955 |
| Bse3DI | GCAATG | 1 cut(s) | 303 |
| Bse8I | GATNNNNATC | 1 cut(s) | 446 |
| BseAI | TCCGGA | 1 cut(s) | 419 |
| BseBI | CCWGG | 1 cut(s) | 618 |
| BseDI | CCNNGG | 4 cut(s) | 133, 1111, 1177, 1178 |
| BseGI | GGATG | 7 cut(s) | 112, 183, 428, 636, 661, 678, 825 |
| BseJI | GATNNNNATC | 1 cut(s) | 446 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 134, 217, 257, 1034, 1088, 1244 |
| BseMI | GCAATG | 1 cut(s) | 303 |
| BseMII | CTCAG | 1 cut(s) | 126 |
| BseNI | ACTGG | 3 cut(s) | 128, 817, 955 |
| BseRI | GAGGAG | 4 cut(s) | 350, 698, 796, 1137 |
| BseSI | GKGCMC | 1 cut(s) | 1156 |
| BseXI | GCAGC | 1 cut(s) | 1182 |
| Bsh1236I | CGCG | 2 cut(s) | 370, 1190 |
| BshFI | GGCC | 2 cut(s) | 138, 1027 |
| BshNI | GGYRCC | 1 cut(s) | 201 |
| BsiHKAI | GWGCWC | 1 cut(s) | 279 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1177 |
| BsiSI | CCGG | 2 cut(s) | 420, 1178 |
| BslFI | GGGAC | 1 cut(s) | 1087 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 134, 217, 257, 1034, 1088, 1244 |
| BsmAI | GTCTC | 2 cut(s) | 64, 520 |
| BsmFI | GGGAC | 1 cut(s) | 1087 |
| BsmI | GAATGC | 1 cut(s) | 387 |
| BsnI | GGCC | 2 cut(s) | 138, 1027 |
| BsoBI | CYCGRG | 1 cut(s) | 1177 |
| Bsp1286I | GDGCHC | 2 cut(s) | 279, 1156 |
| Bsp13I | TCCGGA | 1 cut(s) | 419 |
| Bsp143I | GATC | 4 cut(s) | 313, 447, 488, 552 |
| BspACI | CCGC | 6 cut(s) | 251, 365, 428, 508, 919, 1190 |
| BspANI | GGCC | 2 cut(s) | 138, 1027 |
| BspCNI | CTCAG | 1 cut(s) | 125 |
| BspEI | TCCGGA | 1 cut(s) | 419 |
| BspFNI | CGCG | 2 cut(s) | 370, 1190 |
| BspHI | TCATGA | 1 cut(s) | 831 |
| BspLI | GGNNCC | 3 cut(s) | 203, 339, 1026 |
| BspMAI | CTGCAG | 1 cut(s) | 309 |
| BspPI | GGATC | 3 cut(s) | 308, 496, 560 |
| BspT107I | GGYRCC | 1 cut(s) | 201 |
| BsrDI | GCAATG | 1 cut(s) | 303 |
| BsrI | ACTGG | 3 cut(s) | 128, 817, 955 |
| BssECI | CCNNGG | 4 cut(s) | 133, 1111, 1177, 1178 |
| BssMI | GATC | 4 cut(s) | 313, 447, 488, 552 |
| BssT1I | CCWWGG | 1 cut(s) | 1111 |
| Bst2UI | CCWGG | 1 cut(s) | 618 |
| Bst4CI | ACNGT | 5 cut(s) | 216, 568, 607, 680, 800 |
| Bst6I | CTCTTC | 1 cut(s) | 862 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1139 |
| BstC8I | GCNNGC | 3 cut(s) | 229, 510, 936 |
| BstDEI | CTNAG | 1 cut(s) | 112 |
| BstEII | GGTNACC | 1 cut(s) | 842 |
| BstF5I | GGATG | 7 cut(s) | 112, 183, 428, 636, 661, 678, 825 |
| BstFNI | CGCG | 2 cut(s) | 370, 1190 |
| BstHHI | GCGC | 2 cut(s) | 370, 934 |
| BstKTI | GATC | 4 cut(s) | 316, 450, 491, 555 |
| BstMAI | GTCTC | 2 cut(s) | 64, 520 |
| BstMBI | GATC | 4 cut(s) | 313, 447, 488, 552 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 1004, 1139 |
| BstNI | CCWGG | 1 cut(s) | 618 |
| BstNSI | RCATGY | 1 cut(s) | 544 |
| BstPI | GGTNACC | 1 cut(s) | 842 |
| BstSCI | CCNGG | 3 cut(s) | 616, 1176, 1177 |
| BstSFI | CTRYAG | 4 cut(s) | 196, 305, 318, 796 |
| BstSLI | GKGCMC | 1 cut(s) | 1156 |
| BstUI | CGCG | 2 cut(s) | 370, 1190 |
| BstV1I | GCAGC | 1 cut(s) | 1182 |
| BstV2I | GAAGAC | 1 cut(s) | 985 |
| BstX2I | RGATCY | 1 cut(s) | 488 |
| BstYI | RGATCY | 1 cut(s) | 488 |
| BsuRI | GGCC | 2 cut(s) | 138, 1027 |
| BtrI | CACGTC | 1 cut(s) | 523 |
| BtsCI | GGATG | 7 cut(s) | 112, 183, 428, 636, 661, 678, 825 |
| BtsI | GCAGTG | 1 cut(s) | 604 |
| BtsIMutI | CAGTG | 3 cut(s) | 573, 604, 685 |
| Cac8I | GCNNGC | 3 cut(s) | 229, 510, 936 |
| CaiI | CAGNNNCTG | 1 cut(s) | 611 |
| CciI | TCATGA | 1 cut(s) | 831 |
| CfoI | GCGC | 2 cut(s) | 370, 934 |
| Cfr13I | GGNCC | 2 cut(s) | 614, 1025 |
| Cfr9I | CCCGGG | 1 cut(s) | 1177 |
| CsiI | ACCWGGT | 1 cut(s) | 616 |
| Csp6I | GTAC | 4 cut(s) | 184, 415, 593, 948 |
| CviAII | CATG | 6 cut(s) | 100, 541, 832, 929, 1139, 1199 |
| CviQI | GTAC | 4 cut(s) | 184, 415, 593, 948 |
| DdeI | CTNAG | 1 cut(s) | 112 |
| DpnI | GATC | 4 cut(s) | 315, 449, 490, 554 |
| DpnII | GATC | 4 cut(s) | 313, 447, 488, 552 |
| EaeI | YGGCCR | 1 cut(s) | 136 |
| Eam1104I | CTCTTC | 1 cut(s) | 862 |
| EarI | CTCTTC | 1 cut(s) | 862 |
| Eco130I | CCWWGG | 1 cut(s) | 1111 |
| Eco47I | GGWCC | 1 cut(s) | 614 |
| Eco57I | CTGAAG | 1 cut(s) | 960 |
| Eco88I | CYCGRG | 1 cut(s) | 1177 |
| Eco91I | GGTNACC | 1 cut(s) | 842 |
| EcoO65I | GGTNACC | 1 cut(s) | 842 |
| EcoRI | GAATTC | 1 cut(s) | 578 |
| EcoRII | CCWGG | 1 cut(s) | 616 |
| EcoT14I | CCWWGG | 1 cut(s) | 1111 |
| EcoT22I | ATGCAT | 1 cut(s) | 775 |
| ErhI | CCWWGG | 1 cut(s) | 1111 |
| FaeI | CATG | 6 cut(s) | 103, 544, 835, 932, 1142, 1202 |
| FaqI | GGGAC | 1 cut(s) | 1087 |
| FatI | CATG | 6 cut(s) | 99, 540, 831, 928, 1138, 1198 |
| FauI | CCCGC | 2 cut(s) | 421, 501 |
| FauNDI | CATATG | 1 cut(s) | 255 |
| Fnu4HI | GCNGC | 3 cut(s) | 366, 919, 1196 |
| FokI | GGATG | 7 cut(s) | 119, 190, 435, 643, 648, 685, 832 |
| Fsp4HI | GCNGC | 3 cut(s) | 366, 919, 1196 |
| FspAI | RTGCGCAY | 1 cut(s) | 933 |
| FspI | TGCGCA | 1 cut(s) | 933 |
| GlaI | GCGC | 2 cut(s) | 369, 933 |
| GluI | GCNGC | 3 cut(s) | 366, 919, 1196 |
| GsuI | CTGGAG | 2 cut(s) | 84, 733 |
| HaeIII | GGCC | 2 cut(s) | 138, 1027 |
| HapII | CCGG | 2 cut(s) | 420, 1178 |
| HhaI | GCGC | 2 cut(s) | 370, 934 |
| Hin1II | CATG | 6 cut(s) | 103, 544, 835, 932, 1142, 1202 |
| Hin6I | GCGC | 2 cut(s) | 368, 932 |
| HinP1I | GCGC | 2 cut(s) | 368, 932 |
| HindIII | AAGCTT | 1 cut(s) | 482 |
| HinfI | GANTC | 4 cut(s) | 96, 164, 399, 1042 |
| HpaII | CCGG | 2 cut(s) | 420, 1178 |
| HphI | GGTGA | 2 cut(s) | 854, 1156 |
| Hpy188I | TCNGA | 4 cut(s) | 47, 274, 283, 624 |
| Hpy188III | TCNNGA | 7 cut(s) | 63, 85, 382, 420, 704, 712, 832 |
| HpyAV | CCTTC | 1 cut(s) | 718 |
| HpyCH4III | ACNGT | 5 cut(s) | 216, 568, 607, 680, 800 |
| HpyCH4IV | ACGT | 1 cut(s) | 522 |
| HpyCH4V | TGCA | 6 cut(s) | 231, 307, 754, 773, 1133, 1198 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 1004, 1139 |
| HpyF3I | CTNAG | 1 cut(s) | 112 |
| HpySE526I | ACGT | 1 cut(s) | 522 |
| Hsp92II | CATG | 6 cut(s) | 103, 544, 835, 932, 1142, 1202 |
| HspAI | GCGC | 2 cut(s) | 368, 932 |
| Kpn2I | TCCGGA | 1 cut(s) | 419 |
| Kzo9I | GATC | 4 cut(s) | 313, 447, 488, 552 |
| LmnI | GCTCC | 3 cut(s) | 337, 880, 1247 |
| Lsp1109I | GCAGC | 1 cut(s) | 1182 |
| LweI | GCATC | 1 cut(s) | 385 |
| MabI | ACCWGGT | 1 cut(s) | 616 |
| MaeII | ACGT | 1 cut(s) | 522 |
| MaeIII | GTNAC | 8 cut(s) | 325, 518, 601, 842, 1016, 1049, 1067, 1085 |
| MalI | GATC | 4 cut(s) | 315, 449, 490, 554 |
| MboI | GATC | 4 cut(s) | 313, 447, 488, 552 |
| MboII | GAAGA | 4 cut(s) | 451, 849, 953, 990 |
| MflI | RGATCY | 1 cut(s) | 488 |
| MhlI | GDGCHC | 2 cut(s) | 279, 1156 |
| MluCI | AATT | 9 cut(s) | 261, 530, 578, 625, 922, 1008, 1029, 1077, 1157 |
| MlyI | GAGTC | 2 cut(s) | 158, 1051 |
| MmeI | TCCRAC | 1 cut(s) | 797 |
| Mph1103I | ATGCAT | 1 cut(s) | 775 |
| MroI | TCCGGA | 1 cut(s) | 419 |
| MroXI | GAANNNNTTC | 1 cut(s) | 95 |
| MseI | TTAA | 5 cut(s) | 15, 51, 639, 689, 777 |
| MspI | CCGG | 2 cut(s) | 420, 1178 |
| MspR9I | CCNGG | 3 cut(s) | 618, 1178, 1179 |
| Mva1269I | GAATGC | 1 cut(s) | 387 |
| MvaI | CCWGG | 1 cut(s) | 618 |
| MvnI | CGCG | 2 cut(s) | 370, 1190 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 1004, 1139 |
| NciI | CCSGG | 2 cut(s) | 1178, 1179 |
| NdeI | CATATG | 1 cut(s) | 255 |
| NdeII | GATC | 4 cut(s) | 313, 447, 488, 552 |
| NlaIII | CATG | 6 cut(s) | 103, 544, 835, 932, 1142, 1202 |
| NlaIV | GGNNCC | 3 cut(s) | 203, 339, 1026 |
| NmeAIII | GCCGAG | 1 cut(s) | 114 |
| NmuCI | GTSAC | 4 cut(s) | 325, 518, 601, 842 |
| NsbI | TGCGCA | 1 cut(s) | 933 |
| NsiI | ATGCAT | 1 cut(s) | 775 |
| NspI | RCATGY | 1 cut(s) | 544 |
| PagI | TCATGA | 1 cut(s) | 831 |
| PctI | GAATGC | 1 cut(s) | 387 |
| PdmI | GAANNNNTTC | 1 cut(s) | 95 |
| PfeI | GAWTC | 2 cut(s) | 96, 399 |
| PflFI | GACNNNGTC | 1 cut(s) | 618 |
| PkrI | GCNGC | 3 cut(s) | 367, 920, 1197 |
| PleI | GAGTC | 2 cut(s) | 158, 1050 |
| PpsI | GAGTC | 2 cut(s) | 158, 1050 |
| PsiI | TTATAA | 1 cut(s) | 1229 |
| Psp6I | CCWGG | 1 cut(s) | 616 |
| PspEI | GGTNACC | 1 cut(s) | 842 |
| PspGI | CCWGG | 1 cut(s) | 616 |
| PspN4I | GGNNCC | 3 cut(s) | 203, 339, 1026 |
| PspPI | GGNCC | 2 cut(s) | 614, 1025 |
| PstI | CTGCAG | 1 cut(s) | 309 |
| PstNI | CAGNNNCTG | 1 cut(s) | 611 |
| PsuI | RGATCY | 1 cut(s) | 488 |
| PsyI | GACNNNGTC | 1 cut(s) | 618 |
| RsaI | GTAC | 4 cut(s) | 185, 416, 594, 949 |
| RsaNI | GTAC | 4 cut(s) | 184, 415, 593, 948 |
| SaqAI | TTAA | 5 cut(s) | 15, 51, 639, 689, 777 |
| SatI | GCNGC | 3 cut(s) | 366, 919, 1196 |
| Sau3AI | GATC | 4 cut(s) | 313, 447, 488, 552 |
| Sau96I | GGNCC | 2 cut(s) | 614, 1025 |
| ScaI | AGTACT | 2 cut(s) | 416, 949 |
| SchI | GAGTC | 2 cut(s) | 158, 1051 |
| ScrFI | CCNGG | 3 cut(s) | 618, 1178, 1179 |
| SduI | GDGCHC | 2 cut(s) | 279, 1156 |
| SexAI | ACCWGGT | 1 cut(s) | 616 |
| SfaNI | GCATC | 1 cut(s) | 385 |
| SfcI | CTRYAG | 4 cut(s) | 196, 305, 318, 796 |
| SinI | GGWCC | 1 cut(s) | 614 |
| SmaI | CCCGGG | 1 cut(s) | 1179 |
| SmlI | CTYRAG | 2 cut(s) | 380, 410 |
| SmoI | CTYRAG | 2 cut(s) | 380, 410 |
| Sse9I | AATT | 9 cut(s) | 261, 530, 578, 625, 922, 1008, 1029, 1077, 1157 |
| SsiI | CCGC | 6 cut(s) | 251, 365, 428, 508, 919, 1190 |
| SspI | AATATT | 1 cut(s) | 643 |
| StyD4I | CCNGG | 3 cut(s) | 616, 1176, 1177 |
| StyI | CCWWGG | 1 cut(s) | 1111 |
| TaaI | ACNGT | 5 cut(s) | 216, 568, 607, 680, 800 |
| TaiI | ACGT | 1 cut(s) | 525 |
| TaqI | TCGA | 5 cut(s) | 19, 562, 1040, 1146, 1219 |
| TasI | AATT | 9 cut(s) | 261, 530, 578, 625, 922, 1008, 1029, 1077, 1157 |
| TatI | WGTACW | 2 cut(s) | 414, 947 |
| TauI | GCSGC | 2 cut(s) | 368, 921 |
| TfiI | GAWTC | 2 cut(s) | 96, 399 |
| Tru1I | TTAA | 5 cut(s) | 15, 51, 639, 689, 777 |
| Tru9I | TTAA | 5 cut(s) | 15, 51, 639, 689, 777 |
| TscAI | CASTG | 3 cut(s) | 573, 604, 685 |
| TseFI | GTSAC | 4 cut(s) | 325, 518, 601, 842 |
| TseI | GCWGC | 1 cut(s) | 1195 |
| Tsp45I | GTSAC | 4 cut(s) | 325, 518, 601, 842 |
| TspDTI | ATGAA | 5 cut(s) | 17, 88, 252, 689, 836 |
| TspGWI | ACGGA | 2 cut(s) | 29, 1260 |
| TspMI | CCCGGG | 1 cut(s) | 1177 |
| TspRI | CASTG | 3 cut(s) | 573, 604, 685 |
| Tth111I | GACNNNGTC | 1 cut(s) | 618 |
| VpaK11BI | GGWCC | 1 cut(s) | 614 |
| XapI | RAATTY | 1 cut(s) | 578 |
| XceI | RCATGY | 1 cut(s) | 544 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 624 |
| XmaI | CCCGGG | 1 cut(s) | 1177 |
| XmnI | GAANNNNTTC | 1 cut(s) | 95 |
| ZrmI | AGTACT | 2 cut(s) | 416, 949 |
| Zsp2I | ATGCAT | 1 cut(s) | 775 |