E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 590 |
| AatII | GACGTC | 1 cut(s) | 594 |
| AccI | GTMKAC | 1 cut(s) | 636 |
| AccII | CGCG | 1 cut(s) | 388 |
| AclWI | GGATC | 2 cut(s) | 42, 55 |
| AcoI | YGGCCR | 1 cut(s) | 389 |
| AcsI | RAATTY | 1 cut(s) | 91 |
| AcyI | GRCGYC | 2 cut(s) | 300, 591 |
| AfaI | GTAC | 2 cut(s) | 153, 419 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 445 |
| AjnI | CCWGG | 3 cut(s) | 59, 286, 639 |
| AluBI | AGCT | 2 cut(s) | 190, 347 |
| AluI | AGCT | 2 cut(s) | 190, 347 |
| Alw26I | GTCTC | 1 cut(s) | 306 |
| AlwI | GGATC | 2 cut(s) | 42, 55 |
| AoxI | GGCC | 2 cut(s) | 71, 389 |
| ApeKI | GCWGC | 3 cut(s) | 54, 344, 462 |
| ApoI | RAATTY | 1 cut(s) | 91 |
| AspS9I | GGNCC | 1 cut(s) | 628 |
| AsuHPI | GGTGA | 2 cut(s) | 510, 565 |
| AvaII | GGWCC | 1 cut(s) | 628 |
| BamHI | GGATCC | 1 cut(s) | 47 |
| BanII | GRGCYC | 1 cut(s) | 621 |
| BbsI | GAAGAC | 3 cut(s) | 273, 303, 562 |
| BbvI | GCAGC | 3 cut(s) | 66, 356, 474 |
| BccI | CCATC | 3 cut(s) | 26, 521, 560 |
| BciT130I | CCWGG | 3 cut(s) | 61, 288, 641 |
| BcoDI | GTCTC | 1 cut(s) | 306 |
| BfmI | CTRYAG | 1 cut(s) | 191 |
| Bme1390I | CCNGG | 3 cut(s) | 61, 288, 641 |
| Bme18I | GGWCC | 1 cut(s) | 628 |
| BmgT120I | GGNCC | 1 cut(s) | 628 |
| BmiI | GGNNCC | 3 cut(s) | 49, 504, 630 |
| BmrFI | CCNGG | 3 cut(s) | 61, 288, 641 |
| BmrI | ACTGGG | 1 cut(s) | 409 |
| BmsI | GCATC | 4 cut(s) | 119, 230, 479, 547 |
| BmuI | ACTGGG | 1 cut(s) | 409 |
| BpiI | GAAGAC | 3 cut(s) | 273, 303, 562 |
| Bsa29I | ATCGAT | 1 cut(s) | 555 |
| BsaHI | GRCGYC | 2 cut(s) | 300, 591 |
| BsaJI | CCNNGG | 2 cut(s) | 59, 386 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 483, 513 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 445 |
| Bse1I | ACTGG | 1 cut(s) | 415 |
| BseBI | CCWGG | 3 cut(s) | 61, 288, 641 |
| BseCI | ATCGAT | 1 cut(s) | 555 |
| BseDI | CCNNGG | 2 cut(s) | 59, 386 |
| BseGI | GGATG | 4 cut(s) | 37, 110, 221, 470 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 445 |
| BseMII | CTCAG | 1 cut(s) | 600 |
| BseNI | ACTGG | 1 cut(s) | 415 |
| BseX3I | CGGCCG | 1 cut(s) | 389 |
| BseXI | GCAGC | 3 cut(s) | 66, 356, 474 |
| Bsh1236I | CGCG | 1 cut(s) | 388 |
| Bsh1285I | CGRYCG | 3 cut(s) | 158, 392, 439 |
| BshFI | GGCC | 2 cut(s) | 73, 391 |
| BshVI | ATCGAT | 1 cut(s) | 555 |
| BsiEI | CGRYCG | 3 cut(s) | 158, 392, 439 |
| BsiSI | CCGG | 1 cut(s) | 489 |
| BsiWI | CGTACG | 1 cut(s) | 151 |
| BslFI | GGGAC | 1 cut(s) | 614 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 445 |
| BsmAI | GTCTC | 1 cut(s) | 306 |
| BsmFI | GGGAC | 1 cut(s) | 614 |
| BsmI | GAATGC | 1 cut(s) | 381 |
| BsnI | GGCC | 2 cut(s) | 73, 391 |
| Bsp1286I | GDGCHC | 1 cut(s) | 621 |
| Bsp143I | GATC | 1 cut(s) | 47 |
| BspANI | GGCC | 2 cut(s) | 73, 391 |
| BspCNI | CTCAG | 1 cut(s) | 599 |
| BspDI | ATCGAT | 1 cut(s) | 555 |
| BspFNI | CGCG | 1 cut(s) | 388 |
| BspLI | GGNNCC | 3 cut(s) | 49, 504, 630 |
| BspPI | GGATC | 2 cut(s) | 42, 55 |
| BsrI | ACTGG | 1 cut(s) | 415 |
| BssECI | CCNNGG | 2 cut(s) | 59, 386 |
| BssMI | GATC | 1 cut(s) | 47 |
| BssNI | GRCGYC | 2 cut(s) | 300, 591 |
| Bst2UI | CCWGG | 3 cut(s) | 61, 288, 641 |
| Bst4CI | ACNGT | 4 cut(s) | 86, 199, 440, 628 |
| Bst6I | CTCTTC | 1 cut(s) | 327 |
| BstACI | GRCGYC | 2 cut(s) | 300, 591 |
| BstC8I | GCNNGC | 3 cut(s) | 124, 411, 652 |
| BstDEI | CTNAG | 1 cut(s) | 586 |
| BstDSI | CCRYGG | 1 cut(s) | 386 |
| BstF5I | GGATG | 4 cut(s) | 37, 110, 221, 470 |
| BstFNI | CGCG | 1 cut(s) | 388 |
| BstKTI | GATC | 1 cut(s) | 50 |
| BstMAI | GTCTC | 1 cut(s) | 306 |
| BstMBI | GATC | 1 cut(s) | 47 |
| BstMCI | CGRYCG | 3 cut(s) | 158, 392, 439 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 70, 166, 476, 485, 533, 536, 542, 557 |
| BstNI | CCWGG | 3 cut(s) | 61, 288, 641 |
| BstSCI | CCNGG | 3 cut(s) | 59, 286, 639 |
| BstSFI | CTRYAG | 1 cut(s) | 191 |
| BstUI | CGCG | 1 cut(s) | 388 |
| BstV1I | GCAGC | 3 cut(s) | 66, 356, 474 |
| BstV2I | GAAGAC | 3 cut(s) | 273, 303, 562 |
| BstX2I | RGATCY | 1 cut(s) | 47 |
| BstYI | RGATCY | 1 cut(s) | 47 |
| BstZI | CGGCCG | 1 cut(s) | 389 |
| Bsu15I | ATCGAT | 1 cut(s) | 555 |
| BsuRI | GGCC | 2 cut(s) | 73, 391 |
| BsuTUI | ATCGAT | 1 cut(s) | 555 |
| BtgI | CCRYGG | 1 cut(s) | 386 |
| BtsCI | GGATG | 4 cut(s) | 37, 110, 221, 470 |
| Cac8I | GCNNGC | 3 cut(s) | 124, 411, 652 |
| CciNI | GCGGCCGC | 1 cut(s) | 389 |
| Cfr13I | GGNCC | 1 cut(s) | 628 |
| Cfr42I | CCGCGG | 1 cut(s) | 389 |
| ClaI | ATCGAT | 1 cut(s) | 555 |
| CseI | GACGC | 1 cut(s) | 308 |
| CsiI | ACCWGGT | 1 cut(s) | 639 |
| Csp6I | GTAC | 2 cut(s) | 152, 418 |
| CviJI | RGCY | 8 cut(s) | 57, 73, 190, 347, 391, 465, 503, 619 |
| CviKI_1 | RGCY | 8 cut(s) | 57, 73, 190, 347, 391, 465, 503, 619 |
| CviQI | GTAC | 2 cut(s) | 152, 418 |
| DdeI | CTNAG | 1 cut(s) | 586 |
| DpnI | GATC | 1 cut(s) | 49 |
| DpnII | GATC | 1 cut(s) | 47 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 590 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 590 |
| EaeI | YGGCCR | 1 cut(s) | 389 |
| EagI | CGGCCG | 1 cut(s) | 389 |
| Eam1104I | CTCTTC | 1 cut(s) | 327 |
| EarI | CTCTTC | 1 cut(s) | 327 |
| EciI | GGCGGA | 3 cut(s) | 36, 109, 514 |
| EclXI | CGGCCG | 1 cut(s) | 389 |
| Eco147I | AGGCCT | 1 cut(s) | 73 |
| Eco24I | GRGCYC | 1 cut(s) | 621 |
| Eco47I | GGWCC | 1 cut(s) | 628 |
| Eco52I | CGGCCG | 1 cut(s) | 389 |
| EcoRI | GAATTC | 1 cut(s) | 91 |
| EcoRII | CCWGG | 3 cut(s) | 59, 286, 639 |
| EcoT38I | GRGCYC | 1 cut(s) | 621 |
| FaiI | YATR | 1 cut(s) | 662 |
| FaqI | GGGAC | 1 cut(s) | 614 |
| FauI | CCCGC | 1 cut(s) | 59 |
| FblI | GTMKAC | 1 cut(s) | 636 |
| FokI | GGATG | 4 cut(s) | 44, 97, 208, 457 |
| FriOI | GRGCYC | 1 cut(s) | 621 |
| HaeIII | GGCC | 2 cut(s) | 73, 391 |
| HapII | CCGG | 1 cut(s) | 489 |
| HgaI | GACGC | 1 cut(s) | 308 |
| Hin1I | GRCGYC | 2 cut(s) | 300, 591 |
| HinfI | GANTC | 3 cut(s) | 9, 492, 584 |
| HpaII | CCGG | 1 cut(s) | 489 |
| HphI | GGTGA | 2 cut(s) | 510, 565 |
| Hpy166II | GTNNAC | 1 cut(s) | 637 |
| Hpy188I | TCNGA | 3 cut(s) | 81, 90, 589 |
| Hpy8I | GTNNAC | 1 cut(s) | 637 |
| Hpy99I | CGWCG | 5 cut(s) | 102, 203, 275, 278, 323 |
| HpyAV | CCTTC | 2 cut(s) | 32, 516 |
| HpyCH4III | ACNGT | 4 cut(s) | 86, 199, 440, 628 |
| HpyCH4IV | ACGT | 1 cut(s) | 591 |
| HpyCH4V | TGCA | 1 cut(s) | 470 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 70, 166, 476, 485, 533, 536, 542, 557 |
| HpyF3I | CTNAG | 1 cut(s) | 586 |
| HpySE526I | ACGT | 1 cut(s) | 591 |
| Hsp92I | GRCGYC | 2 cut(s) | 300, 591 |
| KspI | CCGCGG | 1 cut(s) | 389 |
| Kzo9I | GATC | 1 cut(s) | 47 |
| LmnI | GCTCC | 1 cut(s) | 508 |
| Lsp1109I | GCAGC | 3 cut(s) | 66, 356, 474 |
| LweI | GCATC | 4 cut(s) | 119, 230, 479, 547 |
| MabI | ACCWGGT | 1 cut(s) | 639 |
| MaeII | ACGT | 1 cut(s) | 591 |
| MalI | GATC | 1 cut(s) | 49 |
| MboI | GATC | 1 cut(s) | 47 |
| MflI | RGATCY | 1 cut(s) | 47 |
| MhlI | GDGCHC | 1 cut(s) | 621 |
| MluCI | AATT | 2 cut(s) | 91, 351 |
| MlyI | GAGTC | 2 cut(s) | 501, 578 |
| MspA1I | CMGCKG | 2 cut(s) | 347, 388 |
| MspI | CCGG | 1 cut(s) | 489 |
| MspR9I | CCNGG | 3 cut(s) | 61, 288, 641 |
| Mva1269I | GAATGC | 1 cut(s) | 381 |
| MvaI | CCWGG | 3 cut(s) | 61, 288, 641 |
| MvnI | CGCG | 1 cut(s) | 388 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 70, 166, 476, 485, 533, 536, 542, 557 |
| NdeII | GATC | 1 cut(s) | 47 |
| NlaIV | GGNNCC | 3 cut(s) | 49, 504, 630 |
| NotI | GCGGCCGC | 1 cut(s) | 389 |
| PceI | AGGCCT | 1 cut(s) | 73 |
| PctI | GAATGC | 1 cut(s) | 381 |
| PfeI | GAWTC | 1 cut(s) | 9 |
| Pfl23II | CGTACG | 1 cut(s) | 151 |
| PfoI | TCCNGGA | 1 cut(s) | 286 |
| PleI | GAGTC | 2 cut(s) | 500, 578 |
| PpsI | GAGTC | 2 cut(s) | 500, 578 |
| Psp6I | CCWGG | 3 cut(s) | 59, 286, 639 |
| PspGI | CCWGG | 3 cut(s) | 59, 286, 639 |
| PspLI | CGTACG | 1 cut(s) | 151 |
| PspN4I | GGNNCC | 3 cut(s) | 49, 504, 630 |
| PspPI | GGNCC | 1 cut(s) | 628 |
| PsuI | RGATCY | 1 cut(s) | 47 |
| PvuII | CAGCTG | 1 cut(s) | 347 |
| RsaI | GTAC | 2 cut(s) | 153, 419 |
| RsaNI | GTAC | 2 cut(s) | 152, 418 |
| SacII | CCGCGG | 1 cut(s) | 389 |
| Sau3AI | GATC | 1 cut(s) | 47 |
| Sau96I | GGNCC | 1 cut(s) | 628 |
| SchI | GAGTC | 2 cut(s) | 501, 578 |
| ScrFI | CCNGG | 3 cut(s) | 61, 288, 641 |
| SduI | GDGCHC | 1 cut(s) | 621 |
| SetI | ASST | 6 cut(s) | 119, 192, 349, 423, 594, 645 |
| SexAI | ACCWGGT | 1 cut(s) | 639 |
| SfaNI | GCATC | 4 cut(s) | 119, 230, 479, 547 |
| SfcI | CTRYAG | 1 cut(s) | 191 |
| Sfr303I | CCGCGG | 1 cut(s) | 389 |
| SgrBI | CCGCGG | 1 cut(s) | 389 |
| SinI | GGWCC | 1 cut(s) | 628 |
| Sse9I | AATT | 2 cut(s) | 91, 351 |
| SseBI | AGGCCT | 1 cut(s) | 73 |
| StuI | AGGCCT | 1 cut(s) | 73 |
| StyD4I | CCNGG | 3 cut(s) | 59, 286, 639 |
| TaaI | ACNGT | 4 cut(s) | 86, 199, 440, 628 |
| TaiI | ACGT | 1 cut(s) | 594 |
| TaqI | TCGA | 3 cut(s) | 100, 201, 555 |
| TasI | AATT | 2 cut(s) | 91, 351 |
| TfiI | GAWTC | 1 cut(s) | 9 |
| TseI | GCWGC | 3 cut(s) | 54, 344, 462 |
| TspDTI | ATGAA | 4 cut(s) | 228, 309, 345, 595 |
| VpaK11BI | GGWCC | 1 cut(s) | 628 |
| XapI | RAATTY | 1 cut(s) | 91 |
| XmiI | GTMKAC | 1 cut(s) | 636 |
| ZraI | GACGTC | 1 cut(s) | 592 |