E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 3 cut(s) | 428, 680, 944 |
| Acc36I | ACCTGC | 2 cut(s) | 298, 620 |
| AccI | GTMKAC | 1 cut(s) | 1224 |
| AccIII | TCCGGA | 1 cut(s) | 231 |
| AciI | CCGC | 1 cut(s) | 1239 |
| AclWI | GGATC | 3 cut(s) | 223, 256, 269 |
| AcsI | RAATTY | 5 cut(s) | 103, 600, 938, 1075, 1136 |
| AcuI | CTGAAG | 3 cut(s) | 243, 555, 807 |
| AfaI | GTAC | 2 cut(s) | 1207, 1268 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 500, 752 |
| AgsI | TTSAA | 9 cut(s) | 32, 278, 317, 379, 607, 923, 1127, 1142, 1234 |
| AleI | CACNNNNGTG | 2 cut(s) | 395, 647 |
| AluBI | AGCT | 9 cut(s) | 24, 244, 519, 595, 771, 857, 1046, 1184, 1219 |
| AluI | AGCT | 9 cut(s) | 24, 244, 519, 595, 771, 857, 1046, 1184, 1219 |
| Alw21I | GWGCWC | 2 cut(s) | 597, 964 |
| Alw26I | GTCTC | 2 cut(s) | 570, 839 |
| Alw44I | GTGCAC | 1 cut(s) | 960 |
| AlwI | GGATC | 3 cut(s) | 223, 256, 269 |
| Aor13HI | TCCGGA | 1 cut(s) | 231 |
| AoxI | GGCC | 2 cut(s) | 544, 796 |
| ApaLI | GTGCAC | 1 cut(s) | 960 |
| ApeKI | GCWGC | 8 cut(s) | 95, 142, 194, 293, 555, 807, 968, 1081 |
| ApoI | RAATTY | 5 cut(s) | 103, 600, 938, 1075, 1136 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 117 |
| AspS9I | GGNCC | 4 cut(s) | 234, 287, 545, 797 |
| AvaII | GGWCC | 2 cut(s) | 234, 287 |
| BaeGI | GKGCMC | 1 cut(s) | 964 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 748, 781 |
| BamHI | GGATCC | 1 cut(s) | 261 |
| BanII | GRGCYC | 1 cut(s) | 597 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1086, 1118 |
| BbsI | GAAGAC | 1 cut(s) | 1094 |
| Bbv12I | GWGCWC | 2 cut(s) | 597, 964 |
| BbvI | GCAGC | 8 cut(s) | 82, 129, 181, 305, 567, 819, 955, 1068 |
| BccI | CCATC | 3 cut(s) | 896, 1004, 1150 |
| BceAI | ACGGC | 1 cut(s) | 318 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 445, 479, 697, 731 |
| BciVI | GTATCC | 2 cut(s) | 497, 749 |
| BclI | TGATCA | 3 cut(s) | 370, 622, 874 |
| BcoDI | GTCTC | 2 cut(s) | 570, 839 |
| BfaI | CTAG | 2 cut(s) | 1043, 1181 |
| BfmI | CTRYAG | 1 cut(s) | 291 |
| BfuAI | ACCTGC | 2 cut(s) | 298, 620 |
| BfuI | GTATCC | 2 cut(s) | 497, 749 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 139 |
| BisI | GCNGC | 8 cut(s) | 96, 143, 195, 294, 556, 808, 969, 1082 |
| BlsI | GCNGC | 8 cut(s) | 97, 144, 196, 295, 557, 809, 970, 1083 |
| Bme18I | GGWCC | 2 cut(s) | 234, 287 |
| BmgT120I | GGNCC | 4 cut(s) | 234, 287, 545, 797 |
| BmiI | GGNNCC | 2 cut(s) | 219, 263 |
| BmrI | ACTGGG | 1 cut(s) | 243 |
| BmsI | GCATC | 2 cut(s) | 993, 1261 |
| BmuI | ACTGGG | 1 cut(s) | 243 |
| BpiI | GAAGAC | 1 cut(s) | 1094 |
| BpuEI | CTTGAG | 1 cut(s) | 1186 |
| BsaJI | CCNNGG | 1 cut(s) | 134 |
| BsaWI | WCCGGW | 1 cut(s) | 231 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 18, 48, 297, 327 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 500, 752 |
| Bse1I | ACTGG | 2 cut(s) | 249, 969 |
| Bse3DI | GCAATG | 1 cut(s) | 1163 |
| BseAI | TCCGGA | 1 cut(s) | 231 |
| BseDI | CCNNGG | 1 cut(s) | 134 |
| BseGI | GGATG | 1 cut(s) | 181 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 500, 752 |
| BseMI | GCAATG | 1 cut(s) | 1163 |
| BseMII | CTCAG | 3 cut(s) | 882, 1120, 1247 |
| BseNI | ACTGG | 2 cut(s) | 249, 969 |
| BseRI | GAGGAG | 5 cut(s) | 455, 490, 707, 742, 1273 |
| BseSI | GKGCMC | 1 cut(s) | 964 |
| BseXI | GCAGC | 8 cut(s) | 82, 129, 181, 305, 567, 819, 955, 1068 |
| BsgI | GTGCAG | 2 cut(s) | 180, 598 |
| BshFI | GGCC | 2 cut(s) | 546, 798 |
| BsiHKAI | GWGCWC | 2 cut(s) | 597, 964 |
| BsiSI | CCGG | 2 cut(s) | 206, 232 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 500, 752 |
| BsmAI | GTCTC | 2 cut(s) | 570, 839 |
| BsmBI | CGTCTC | 1 cut(s) | 839 |
| BsnI | GGCC | 2 cut(s) | 546, 798 |
| Bsp1286I | GDGCHC | 2 cut(s) | 597, 964 |
| Bsp13I | TCCGGA | 1 cut(s) | 231 |
| Bsp143I | GATC | 5 cut(s) | 228, 261, 370, 622, 874 |
| BspACI | CCGC | 1 cut(s) | 1239 |
| BspANI | GGCC | 2 cut(s) | 546, 798 |
| BspCNI | CTCAG | 3 cut(s) | 881, 1119, 1248 |
| BspEI | TCCGGA | 1 cut(s) | 231 |
| BspLI | GGNNCC | 2 cut(s) | 219, 263 |
| BspMAI | CTGCAG | 1 cut(s) | 295 |
| BspMI | ACCTGC | 2 cut(s) | 298, 620 |
| BspPI | GGATC | 3 cut(s) | 223, 256, 269 |
| BsrDI | GCAATG | 1 cut(s) | 1163 |
| BsrI | ACTGG | 2 cut(s) | 249, 969 |
| BssECI | CCNNGG | 1 cut(s) | 134 |
| BssMI | GATC | 5 cut(s) | 228, 261, 370, 622, 874 |
| BssT1I | CCWWGG | 1 cut(s) | 134 |
| Bst4CI | ACNGT | 4 cut(s) | 367, 619, 850, 992 |
| Bst6I | CTCTTC | 3 cut(s) | 531, 783, 903 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 968 |
| BstC8I | GCNNGC | 1 cut(s) | 1119 |
| BstDEI | CTNAG | 5 cut(s) | 99, 868, 1085, 1106, 1256 |
| BstF5I | GGATG | 1 cut(s) | 181 |
| BstKTI | GATC | 5 cut(s) | 231, 264, 373, 625, 877 |
| BstMAI | GTCTC | 2 cut(s) | 570, 839 |
| BstMBI | GATC | 5 cut(s) | 228, 261, 370, 622, 874 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 95, 139, 148, 345, 552, 804, 968 |
| BstSFI | CTRYAG | 1 cut(s) | 291 |
| BstSLI | GKGCMC | 1 cut(s) | 964 |
| BstV1I | GCAGC | 8 cut(s) | 82, 129, 181, 305, 567, 819, 955, 1068 |
| BstV2I | GAAGAC | 1 cut(s) | 1094 |
| BstX2I | RGATCY | 1 cut(s) | 261 |
| BstYI | RGATCY | 1 cut(s) | 261 |
| BsuI | GTATCC | 2 cut(s) | 497, 749 |
| BsuRI | GGCC | 2 cut(s) | 546, 798 |
| BtsCI | GGATG | 1 cut(s) | 181 |
| BtsIMutI | CAGTG | 6 cut(s) | 256, 363, 615, 637, 962, 997 |
| BveI | ACCTGC | 2 cut(s) | 298, 620 |
| Cac8I | GCNNGC | 1 cut(s) | 1119 |
| Cfr13I | GGNCC | 4 cut(s) | 234, 287, 545, 797 |
| CseI | GACGC | 2 cut(s) | 561, 813 |
| Csp6I | GTAC | 2 cut(s) | 1206, 1267 |
| CviAII | CATG | 8 cut(s) | 39, 110, 457, 569, 709, 821, 878, 1295 |
| CviQI | GTAC | 2 cut(s) | 1206, 1267 |
| DdeI | CTNAG | 5 cut(s) | 99, 868, 1085, 1106, 1256 |
| DpnI | GATC | 5 cut(s) | 230, 263, 372, 624, 876 |
| DpnII | GATC | 5 cut(s) | 228, 261, 370, 622, 874 |
| Eam1104I | CTCTTC | 3 cut(s) | 531, 783, 903 |
| EarI | CTCTTC | 3 cut(s) | 531, 783, 903 |
| Ecl136II | GAGCTC | 1 cut(s) | 595 |
| Eco130I | CCWWGG | 1 cut(s) | 134 |
| Eco24I | GRGCYC | 1 cut(s) | 597 |
| Eco47I | GGWCC | 2 cut(s) | 234, 287 |
| Eco53kI | GAGCTC | 1 cut(s) | 595 |
| Eco57I | CTGAAG | 3 cut(s) | 243, 555, 807 |
| EcoICRI | GAGCTC | 1 cut(s) | 595 |
| EcoO109I | RGGNCCY | 1 cut(s) | 287 |
| EcoRI | GAATTC | 1 cut(s) | 1075 |
| EcoT14I | CCWWGG | 1 cut(s) | 134 |
| EcoT38I | GRGCYC | 1 cut(s) | 597 |
| ErhI | CCWWGG | 1 cut(s) | 134 |
| Esp3I | CGTCTC | 1 cut(s) | 839 |
| FaeI | CATG | 8 cut(s) | 42, 113, 460, 572, 712, 824, 881, 1298 |
| FatI | CATG | 8 cut(s) | 38, 109, 456, 568, 708, 820, 877, 1294 |
| FbaI | TGATCA | 3 cut(s) | 370, 622, 874 |
| FblI | GTMKAC | 1 cut(s) | 1224 |
| Fnu4HI | GCNGC | 8 cut(s) | 96, 143, 195, 294, 556, 808, 969, 1082 |
| FokI | GGATG | 1 cut(s) | 168 |
| FriOI | GRGCYC | 1 cut(s) | 597 |
| Fsp4HI | GCNGC | 8 cut(s) | 96, 143, 195, 294, 556, 808, 969, 1082 |
| FspBI | CTAG | 2 cut(s) | 1043, 1181 |
| GluI | GCNGC | 8 cut(s) | 96, 143, 195, 294, 556, 808, 969, 1082 |
| HaeIII | GGCC | 2 cut(s) | 546, 798 |
| HapII | CCGG | 2 cut(s) | 206, 232 |
| HgaI | GACGC | 2 cut(s) | 561, 813 |
| Hin1II | CATG | 8 cut(s) | 42, 113, 460, 572, 712, 824, 881, 1298 |
| HincII | GTYRAC | 1 cut(s) | 1225 |
| HindII | GTYRAC | 1 cut(s) | 1225 |
| HindIII | AAGCTT | 4 cut(s) | 22, 242, 517, 769 |
| HinfI | GANTC | 3 cut(s) | 462, 714, 1127 |
| HpaII | CCGG | 2 cut(s) | 206, 232 |
| Hpy166II | GTNNAC | 3 cut(s) | 664, 962, 1225 |
| Hpy188III | TCNNGA | 6 cut(s) | 232, 533, 785, 844, 1000, 1142 |
| Hpy8I | GTNNAC | 3 cut(s) | 664, 962, 1225 |
| Hpy99I | CGWCG | 3 cut(s) | 339, 555, 807 |
| HpyAV | CCTTC | 5 cut(s) | 208, 272, 504, 756, 1104 |
| HpyCH4III | ACNGT | 4 cut(s) | 367, 619, 850, 992 |
| HpyCH4IV | ACGT | 2 cut(s) | 862, 1265 |
| HpyCH4V | TGCA | 7 cut(s) | 109, 151, 197, 293, 615, 962, 1051 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 95, 139, 148, 345, 552, 804, 968 |
| HpyF3I | CTNAG | 5 cut(s) | 99, 868, 1085, 1106, 1256 |
| HpySE526I | ACGT | 2 cut(s) | 862, 1265 |
| Hsp92II | CATG | 8 cut(s) | 42, 113, 460, 572, 712, 824, 881, 1298 |
| Kpn2I | TCCGGA | 1 cut(s) | 231 |
| Ksp22I | TGATCA | 3 cut(s) | 370, 622, 874 |
| Kzo9I | GATC | 5 cut(s) | 228, 261, 370, 622, 874 |
| LmnI | GCTCC | 1 cut(s) | 600 |
| Lsp1109I | GCAGC | 8 cut(s) | 82, 129, 181, 305, 567, 819, 955, 1068 |
| LweI | GCATC | 2 cut(s) | 993, 1261 |
| MaeI | CTAG | 2 cut(s) | 1043, 1181 |
| MaeII | ACGT | 2 cut(s) | 862, 1265 |
| MalI | GATC | 5 cut(s) | 230, 263, 372, 624, 876 |
| MboI | GATC | 5 cut(s) | 228, 261, 370, 622, 874 |
| MboII | GAAGA | 7 cut(s) | 157, 548, 800, 920, 1031, 1094, 1268 |
| MflI | RGATCY | 1 cut(s) | 261 |
| MhlI | GDGCHC | 2 cut(s) | 597, 964 |
| MroI | TCCGGA | 1 cut(s) | 231 |
| MroXI | GAANNNNTTC | 1 cut(s) | 117 |
| MseI | TTAA | 3 cut(s) | 239, 321, 891 |
| MslI | CAYNNNNRTG | 5 cut(s) | 395, 573, 647, 825, 1001 |
| MspI | CCGG | 2 cut(s) | 206, 232 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 95, 139, 148, 345, 552, 804, 968 |
| NdeII | GATC | 5 cut(s) | 228, 261, 370, 622, 874 |
| NlaIII | CATG | 8 cut(s) | 42, 113, 460, 572, 712, 824, 881, 1298 |
| NlaIV | GGNNCC | 2 cut(s) | 219, 263 |
| OliI | CACNNNNGTG | 2 cut(s) | 395, 647 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1221 |
| PdmI | GAANNNNTTC | 1 cut(s) | 117 |
| PfeI | GAWTC | 3 cut(s) | 462, 714, 1127 |
| PkrI | GCNGC | 8 cut(s) | 97, 144, 196, 295, 557, 809, 970, 1083 |
| PpuMI | RGGWCCY | 1 cut(s) | 287 |
| PsiI | TTATAA | 3 cut(s) | 428, 680, 944 |
| Psp124BI | GAGCTC | 1 cut(s) | 597 |
| Psp5II | RGGWCCY | 1 cut(s) | 287 |
| PspN4I | GGNNCC | 2 cut(s) | 219, 263 |
| PspPI | GGNCC | 4 cut(s) | 234, 287, 545, 797 |
| PspPPI | RGGWCCY | 1 cut(s) | 287 |
| PstI | CTGCAG | 1 cut(s) | 295 |
| PsuI | RGATCY | 1 cut(s) | 261 |
| RsaI | GTAC | 2 cut(s) | 1207, 1268 |
| RsaNI | GTAC | 2 cut(s) | 1206, 1267 |
| RseI | CAYNNNNRTG | 5 cut(s) | 395, 573, 647, 825, 1001 |
| SacI | GAGCTC | 1 cut(s) | 597 |
| SalI | GTCGAC | 1 cut(s) | 1223 |
| SaqAI | TTAA | 3 cut(s) | 239, 321, 891 |
| SatI | GCNGC | 8 cut(s) | 96, 143, 195, 294, 556, 808, 969, 1082 |
| Sau3AI | GATC | 5 cut(s) | 228, 261, 370, 622, 874 |
| Sau96I | GGNCC | 4 cut(s) | 234, 287, 545, 797 |
| SduI | GDGCHC | 2 cut(s) | 597, 964 |
| SfaNI | GCATC | 2 cut(s) | 993, 1261 |
| SfcI | CTRYAG | 1 cut(s) | 291 |
| SinI | GGWCC | 2 cut(s) | 234, 287 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 395, 573, 647, 825, 1001 |
| SmlI | CTYRAG | 1 cut(s) | 1201 |
| SmoI | CTYRAG | 1 cut(s) | 1201 |
| SsiI | CCGC | 1 cut(s) | 1239 |
| SspMI | CTAG | 2 cut(s) | 1043, 1181 |
| SstI | GAGCTC | 1 cut(s) | 597 |
| StyI | CCWWGG | 1 cut(s) | 134 |
| TaaI | ACNGT | 4 cut(s) | 367, 619, 850, 992 |
| TaiI | ACGT | 2 cut(s) | 865, 1268 |
| TaqI | TCGA | 5 cut(s) | 550, 591, 802, 843, 1224 |
| TatI | WGTACW | 1 cut(s) | 1205 |
| TfiI | GAWTC | 3 cut(s) | 462, 714, 1127 |
| Tru1I | TTAA | 3 cut(s) | 239, 321, 891 |
| Tru9I | TTAA | 3 cut(s) | 239, 321, 891 |
| TscAI | CASTG | 6 cut(s) | 256, 370, 622, 637, 969, 997 |
| TseI | GCWGC | 8 cut(s) | 95, 142, 194, 293, 555, 807, 968, 1081 |
| TspDTI | ATGAA | 6 cut(s) | 126, 227, 347, 528, 1019, 1226 |
| TspRI | CASTG | 6 cut(s) | 256, 370, 622, 637, 969, 997 |
| VneI | GTGCAC | 1 cut(s) | 960 |
| VpaK11BI | GGWCC | 2 cut(s) | 234, 287 |
| XapI | RAATTY | 5 cut(s) | 103, 600, 938, 1075, 1136 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 88, 307 |
| XmiI | GTMKAC | 1 cut(s) | 1224 |
| XmnI | GAANNNNTTC | 1 cut(s) | 117 |
| XspI | CTAG | 2 cut(s) | 1043, 1181 |