E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 440, 501 |
| AclWI | GGATC | 1 cut(s) | 521 |
| AcoI | YGGCCR | 2 cut(s) | 178, 502 |
| AfaI | GTAC | 2 cut(s) | 18, 310 |
| AflIII | ACRYGT | 1 cut(s) | 488 |
| AgsI | TTSAA | 2 cut(s) | 216, 403 |
| AjnI | CCWGG | 2 cut(s) | 259, 481 |
| AluBI | AGCT | 1 cut(s) | 436 |
| AluI | AGCT | 1 cut(s) | 436 |
| Alw26I | GTCTC | 1 cut(s) | 515 |
| AlwI | GGATC | 1 cut(s) | 521 |
| AoxI | GGCC | 2 cut(s) | 178, 502 |
| ApeKI | GCWGC | 1 cut(s) | 569 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 490 |
| AspS9I | GGNCC | 1 cut(s) | 44 |
| AsuC2I | CCSGG | 1 cut(s) | 270 |
| AvaII | GGWCC | 1 cut(s) | 44 |
| BbvI | GCAGC | 1 cut(s) | 556 |
| BccI | CCATC | 1 cut(s) | 233 |
| BceAI | ACGGC | 2 cut(s) | 193, 489 |
| BciT130I | CCWGG | 2 cut(s) | 261, 483 |
| BclI | TGATCA | 1 cut(s) | 424 |
| BcnI | CCSGG | 1 cut(s) | 270 |
| BcoDI | GTCTC | 1 cut(s) | 515 |
| BfaI | CTAG | 1 cut(s) | 530 |
| BisI | GCNGC | 3 cut(s) | 441, 502, 570 |
| BlsI | GCNGC | 3 cut(s) | 442, 503, 571 |
| Bme1390I | CCNGG | 3 cut(s) | 261, 270, 483 |
| Bme18I | GGWCC | 1 cut(s) | 44 |
| BmgT120I | GGNCC | 1 cut(s) | 44 |
| BmiI | GGNNCC | 1 cut(s) | 46 |
| BmrFI | CCNGG | 3 cut(s) | 261, 270, 483 |
| BmsI | GCATC | 1 cut(s) | 465 |
| BpuMI | CCSGG | 1 cut(s) | 270 |
| Bse1I | ACTGG | 1 cut(s) | 418 |
| Bse3DI | GCAATG | 2 cut(s) | 19, 368 |
| BseBI | CCWGG | 2 cut(s) | 261, 483 |
| BseGI | GGATG | 1 cut(s) | 265 |
| BseMI | GCAATG | 2 cut(s) | 19, 368 |
| BseNI | ACTGG | 1 cut(s) | 418 |
| BseX3I | CGGCCG | 1 cut(s) | 502 |
| BseXI | GCAGC | 1 cut(s) | 556 |
| BseYI | CCCAGC | 1 cut(s) | 47 |
| Bsh1285I | CGRYCG | 1 cut(s) | 505 |
| BshFI | GGCC | 2 cut(s) | 180, 504 |
| BsiEI | CGRYCG | 1 cut(s) | 505 |
| BsiSI | CCGG | 1 cut(s) | 270 |
| BsmAI | GTCTC | 1 cut(s) | 515 |
| BsnI | GGCC | 2 cut(s) | 180, 504 |
| Bsp1407I | TGTACA | 1 cut(s) | 16 |
| Bsp143I | GATC | 5 cut(s) | 106, 124, 184, 424, 513 |
| BspACI | CCGC | 2 cut(s) | 440, 501 |
| BspANI | GGCC | 2 cut(s) | 180, 504 |
| BspLI | GGNNCC | 1 cut(s) | 46 |
| BspPI | GGATC | 1 cut(s) | 521 |
| BsrDI | GCAATG | 2 cut(s) | 19, 368 |
| BsrGI | TGTACA | 1 cut(s) | 16 |
| BsrI | ACTGG | 1 cut(s) | 418 |
| BssMI | GATC | 5 cut(s) | 106, 124, 184, 424, 513 |
| Bst2UI | CCWGG | 2 cut(s) | 261, 483 |
| BstAUI | TGTACA | 1 cut(s) | 16 |
| BstC8I | GCNNGC | 3 cut(s) | 53, 438, 445 |
| BstDEI | CTNAG | 1 cut(s) | 66 |
| BstF5I | GGATG | 1 cut(s) | 265 |
| BstKTI | GATC | 5 cut(s) | 109, 127, 187, 427, 516 |
| BstMAI | GTCTC | 1 cut(s) | 515 |
| BstMBI | GATC | 5 cut(s) | 106, 124, 184, 424, 513 |
| BstMCI | CGRYCG | 1 cut(s) | 505 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 387, 453 |
| BstNI | CCWGG | 2 cut(s) | 261, 483 |
| BstNSI | RCATGY | 1 cut(s) | 492 |
| BstSCI | CCNGG | 3 cut(s) | 259, 268, 481 |
| BstV1I | GCAGC | 1 cut(s) | 556 |
| BstZI | CGGCCG | 1 cut(s) | 502 |
| BsuRI | GGCC | 2 cut(s) | 180, 504 |
| BtsCI | GGATG | 1 cut(s) | 265 |
| BtsIMutI | CAGTG | 1 cut(s) | 425 |
| Cac8I | GCNNGC | 3 cut(s) | 53, 438, 445 |
| Cfr13I | GGNCC | 1 cut(s) | 44 |
| Csp6I | GTAC | 2 cut(s) | 17, 309 |
| CviAII | CATG | 3 cut(s) | 315, 453, 489 |
| CviJI | RGCY | 8 cut(s) | 51, 180, 193, 259, 436, 443, 504, 569 |
| CviKI_1 | RGCY | 8 cut(s) | 51, 180, 193, 259, 436, 443, 504, 569 |
| CviQI | GTAC | 2 cut(s) | 17, 309 |
| DdeI | CTNAG | 1 cut(s) | 66 |
| DpnI | GATC | 5 cut(s) | 108, 126, 186, 426, 515 |
| DpnII | GATC | 5 cut(s) | 106, 124, 184, 424, 513 |
| EaeI | YGGCCR | 2 cut(s) | 178, 502 |
| EagI | CGGCCG | 1 cut(s) | 502 |
| EclXI | CGGCCG | 1 cut(s) | 502 |
| Eco47I | GGWCC | 1 cut(s) | 44 |
| Eco52I | CGGCCG | 1 cut(s) | 502 |
| EcoO109I | RGGNCCY | 1 cut(s) | 44 |
| EcoRII | CCWGG | 2 cut(s) | 259, 481 |
| EcoT22I | ATGCAT | 1 cut(s) | 458 |
| FaeI | CATG | 3 cut(s) | 318, 456, 492 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 529, 561 |
| FatI | CATG | 3 cut(s) | 314, 452, 488 |
| FbaI | TGATCA | 1 cut(s) | 424 |
| Fnu4HI | GCNGC | 3 cut(s) | 441, 502, 570 |
| FokI | GGATG | 1 cut(s) | 252 |
| Fsp4HI | GCNGC | 3 cut(s) | 441, 502, 570 |
| FspBI | CTAG | 1 cut(s) | 530 |
| GluI | GCNGC | 3 cut(s) | 441, 502, 570 |
| GsaI | CCCAGC | 1 cut(s) | 51 |
| HaeIII | GGCC | 2 cut(s) | 180, 504 |
| HapII | CCGG | 1 cut(s) | 270 |
| Hin1II | CATG | 3 cut(s) | 318, 456, 492 |
| HinfI | GANTC | 3 cut(s) | 212, 302, 533 |
| HpaII | CCGG | 1 cut(s) | 270 |
| Hpy188I | TCNGA | 2 cut(s) | 61, 513 |
| Hpy188III | TCNNGA | 5 cut(s) | 80, 128, 188, 520, 530 |
| HpyAV | CCTTC | 1 cut(s) | 84 |
| HpyCH4IV | ACGT | 1 cut(s) | 274 |
| HpyCH4V | TGCA | 3 cut(s) | 390, 456, 572 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 387, 453 |
| HpyF3I | CTNAG | 1 cut(s) | 66 |
| HpySE526I | ACGT | 1 cut(s) | 274 |
| Hsp92II | CATG | 3 cut(s) | 318, 456, 492 |
| Ksp22I | TGATCA | 1 cut(s) | 424 |
| Kzo9I | GATC | 5 cut(s) | 106, 124, 184, 424, 513 |
| Lsp1109I | GCAGC | 1 cut(s) | 556 |
| LweI | GCATC | 1 cut(s) | 465 |
| MaeI | CTAG | 1 cut(s) | 530 |
| MaeII | ACGT | 1 cut(s) | 274 |
| MalI | GATC | 5 cut(s) | 108, 126, 186, 426, 515 |
| MboI | GATC | 5 cut(s) | 106, 124, 184, 424, 513 |
| MluCI | AATT | 3 cut(s) | 329, 384, 525 |
| MmeI | TCCRAC | 1 cut(s) | 491 |
| MnlI | CCTC | 1 cut(s) | 35 |
| Mph1103I | ATGCAT | 1 cut(s) | 458 |
| MroXI | GAANNNNTTC | 1 cut(s) | 490 |
| MseI | TTAA | 3 cut(s) | 251, 356, 471 |
| MspI | CCGG | 1 cut(s) | 270 |
| MspR9I | CCNGG | 3 cut(s) | 261, 270, 483 |
| MvaI | CCWGG | 2 cut(s) | 261, 483 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 387, 453 |
| NciI | CCSGG | 1 cut(s) | 270 |
| NdeII | GATC | 5 cut(s) | 106, 124, 184, 424, 513 |
| NlaIII | CATG | 3 cut(s) | 318, 456, 492 |
| NlaIV | GGNNCC | 1 cut(s) | 46 |
| NsiI | ATGCAT | 1 cut(s) | 458 |
| NspI | RCATGY | 1 cut(s) | 492 |
| PciI | ACATGT | 1 cut(s) | 488 |
| PdmI | GAANNNNTTC | 1 cut(s) | 490 |
| PfeI | GAWTC | 3 cut(s) | 212, 302, 533 |
| PflFI | GACNNNGTC | 1 cut(s) | 276 |
| PfoI | TCCNGGA | 1 cut(s) | 268 |
| PkrI | GCNGC | 3 cut(s) | 442, 503, 571 |
| PpuMI | RGGWCCY | 1 cut(s) | 44 |
| PscI | ACATGT | 1 cut(s) | 488 |
| Psp5II | RGGWCCY | 1 cut(s) | 44 |
| Psp6I | CCWGG | 2 cut(s) | 259, 481 |
| PspFI | CCCAGC | 1 cut(s) | 47 |
| PspGI | CCWGG | 2 cut(s) | 259, 481 |
| PspN4I | GGNNCC | 1 cut(s) | 46 |
| PspPI | GGNCC | 1 cut(s) | 44 |
| PspPPI | RGGWCCY | 1 cut(s) | 44 |
| PsyI | GACNNNGTC | 1 cut(s) | 276 |
| RsaI | GTAC | 2 cut(s) | 18, 310 |
| RsaNI | GTAC | 2 cut(s) | 17, 309 |
| SaqAI | TTAA | 3 cut(s) | 251, 356, 471 |
| SatI | GCNGC | 3 cut(s) | 441, 502, 570 |
| Sau3AI | GATC | 5 cut(s) | 106, 124, 184, 424, 513 |
| Sau96I | GGNCC | 1 cut(s) | 44 |
| ScrFI | CCNGG | 3 cut(s) | 261, 270, 483 |
| SfaNI | GCATC | 1 cut(s) | 465 |
| SinI | GGWCC | 1 cut(s) | 44 |
| Sse9I | AATT | 3 cut(s) | 329, 384, 525 |
| SsiI | CCGC | 2 cut(s) | 440, 501 |
| SspMI | CTAG | 1 cut(s) | 530 |
| StyD4I | CCNGG | 3 cut(s) | 259, 268, 481 |
| TaiI | ACGT | 1 cut(s) | 277 |
| TaqI | TCGA | 2 cut(s) | 519, 552 |
| TasI | AATT | 3 cut(s) | 329, 384, 525 |
| TatI | WGTACW | 2 cut(s) | 16, 308 |
| TauI | GCSGC | 2 cut(s) | 443, 504 |
| TfiI | GAWTC | 3 cut(s) | 212, 302, 533 |
| Tru1I | TTAA | 3 cut(s) | 251, 356, 471 |
| Tru9I | TTAA | 3 cut(s) | 251, 356, 471 |
| TscAI | CASTG | 1 cut(s) | 425 |
| TseI | GCWGC | 1 cut(s) | 569 |
| TspDTI | ATGAA | 2 cut(s) | 17, 45 |
| TspRI | CASTG | 1 cut(s) | 425 |
| Tth111I | GACNNNGTC | 1 cut(s) | 276 |
| VpaK11BI | GGWCC | 1 cut(s) | 44 |
| XbaI | TCTAGA | 1 cut(s) | 529 |
| XceI | RCATGY | 1 cut(s) | 492 |
| XmnI | GAANNNNTTC | 1 cut(s) | 490 |
| XspI | CTAG | 1 cut(s) | 530 |
| Zsp2I | ATGCAT | 1 cut(s) | 458 |