Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0000395

Physical Location & Seq

Genomic Coordinates Forward (+)

33300342 .. 33306408

Gene Structure 6067 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1260400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1806 bp

ATGATGGTAAATGCTGAAGAAAATGTTGTGTTACAAGGGAAGACCAAATGGAAGAAGAAGAAGCAAGTTACCACTCCTCGTCCTGCATGCTCATGGGTGTATTTTAGTCGGGATTTCATCAAGGAATACAGCGCTTCCCATCCTGAATCCTCTGGCCTTAAAGCTGCCACGAAGGCAGCTTCAGATGCATGGAAGTCTATGAGCCTTGATGAGAAAGAAAAATACACAAGGCGTGCTCGTGAAGTGTGGGATAACTACTTGAGTACAGCTCCAACCCGCACCCCTAAGCCAAGAAAACAGGCTAAACTAGTCACAAGATGTTCTCCTGGCCGCTTATTCAATGTGATACAACGTCTTACAAATGAACAGAAGGCTGCAGTGAAAAGCATGGGATTTGGGAGCCTTCTTGACCTCAGATGCCGAACTCTCCGCCGCAGCTTGTGTCTTTGGCTCCTGGAGAGGTTTAACACTACACGATGTAGCTTGGAGATTTGTGGTGAGCGTATTCCTATAACACCAAAAGATGTGGAGCTTGTGATGGGATTAGCAGCTAGTGGGAAGGATGTGGTAAACTCAGGGTCTGATGACTTGATTGCAGATTTGCGGCACAGTTATGATGCTACAAATCATGGTATTTCAGTCAGACTCCTAGAGGAGAGGTTGGCACAGCCTGAAGCAGGAGAGGAATTTAAGAGATCGTTTGTCCTCTATGCACTGGGCACGCTTTTGTCCCCAACAGCAAGGCTGGATGTTAGCCCTTCATTCCTTCACTTTTTGACAAATATGGATGTTGTCCATCAGTACAATTGGGGGAAATTCTTACTTGACCGGCTAGTTTGCGAGGTATCTCGTTTTCGTCAAGGGAAGCAACGTGCGGTGGGTGGCTGTCTTTTGTTTCTCCAGCTCTTTTATTATGAAAGCATCTCTGTTGAGGAGAATGGTGCATTGGCCCCTGCCCTTGTTCCATCCTTATCTTCATGGGGTGAGGACGAGATTACTGAAAGGGAGAAGCGGGAAAGAGAGCTTGGTGGCTATGGATGCGGGGAGGTGGTGTGTAAGGAGAGGTGTCTTGATCTGGATTCTTGCGAGTTTATGGGCCAATTGGATAGGCCACCACCAGCATGCAGTACAAATCATGGGGTTGAGGATGATACTCTCCTTGAGCATAAGGAGAACAAGGAAAATATGCTAGTCTTTGTTGGGAATAAGGACATAGTGTGTGGTGACATTGAACTAGTAGTTGAATCAGTCAGAACACCGTGTCGGAACAGAGAGTATGGGTGTGAGGAAATAGTGGATTACATGAAGAATATTGACCATGAAGAAGCATGCATCTATGCACCATGTCCATGCCCACTTCCAGACTGCAACTTTGTAAGCTCATTTGAACAGTTGTCACTGCATTTCAGCAGTAAGCATTGGGATTCTGGAAGGCGTTTCAGGTACAGCACCCCTTTAGCTGTCTCATTGGGGATAAACGAACAACTTCTCGTTCTTCAAGCAGAGGAGGATGGCGCTCTCTTTCTACTCAACAAGGGTACAGAAAGTATTGGAAACACAATCATGATAACTTCAATCAGACCAATTTCTTCAGGGGAAAGGTTTGCGTATGACCTTGTATCAATACGTGGAAGCAGTTCTCTCAGATTAAAATCAGTGGCAGAAAACTTTCCAGGCGGGGTGGAAGGATTTCGCCCAATGGACTTTCTTCTGGTTCCATTTTGTTTTCTCACCGCAGCCGGGCAACTGAACCTAGATGTGTGTATACAGAATTCTAGTGAGCTCGGTGCATATGTCCTTGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04013 ko04115 ko04120 ko04310 map04013 map04115 map04120 map04310

Pfam Domains

Protein Families

HMG_box PMD Sina

Protein Analysis

602

Amino Acids

67.5

Weight (kDa)

5.86

Isoelectric Point (pI)

43.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMG_box	PF00505	26 - 89	6.7e-08	HMG (high mobility group) box
HMG_box_2	PF09011	31 - 91	3.2e-06	HMG-box domain
PMD	PF10536	165 - 310	1.1e-09	Plant mobile domain
Sina_ZnF	PF21361	413 - 473	1.7e-12	Sina, zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000395)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G66610 AT1G66610 AT1G66620 AT1G66630 AT1G66650 AT1G66660 AT1G66660 AT5G37870 AT5G37890 AT5G37900 AT5G37910 AT5G37930 AT5G62800 AT5G62800
fragaria_vesca	FvH4_1g29100 FvH4_1g29100 FvH4_1g29100 FvH4_1g29100 FvH4_1g29100 FvH4_1g29100 FvH4_1g29100 FvH4_2g03850 FvH4_2g04470 FvH4_2g04471
malus_domestica	MD09G1260200.v1.1 MD09G1260400.v1.1 MD10G1106400.v1.1
prunus_persica	Prupe.3G152000_v2.0.a1 Prupe.3G152100_v2.0.a1 Prupe.3G152100_v2.0.a1 Prupe.3G152100_v2.0.a1 Prupe.8G145400_v2.0.a1 Prupe.8G145500_v2.0.a1
pyrus_communis	pycom09g17480 pycom09g17490 pycom10g09160
rosa_chinensis	RchiOBHm_Chr2g0111691 RchiOBHm_Chr3g0448681 RchiOBHm_Chr3g0493571 RchiOBHm_Chr6g0252861 RchiOBHm_Chr6g0252871 RchiOBHm_Chr6g0253841 RchiOBHm_Chr6g0253921 RchiOBHm_Chr6g0253941
rosa_laevigata	RLG00000007595 RLG00000014953 RLG00000014955 RLG00000014957 RLG00000014958 RLG00000015058 RLG00000017917 RLG00000022703
rosa_multiflora	Rmu_sc0000829.1_g000012 Rmu_sc0000829.1_g000015 Rmu_sc0000829.1_g000016 Rmu_sc0000927.1_g000006 Rmu_sc0001716.1_g000004 Rmu_sc0003921.1_g000007
rosa_roxburghii	Rroxscaffold_4G00307570 Rroxscaffold_6G00391470 Rroxscaffold_6G00426970 Rroxscaffold_7G00211390 Rroxscaffold_7G00211420 Rroxscaffold_7G00211430 Rroxscaffold_7G00212350 Rroxscaffold_7G00212370
rosa_rugosa	Rorug02G0175400 Rorug02G0615300 Rorug03G0262600 Rorug03G0262700 Rorug05G0539200 Rorug05G0539400 Rorug05G0548300 Rorug05G0548400 Rorug05G0548500 Rorug05G0548600
rosa_samantha	Rh2BG240600 Rh3AG014100 Rh3AG311400 Rh3BG013900 Rh3BG347300 Rh3CG345400 Rh3DG347600 Rh6AG055200 Rh6AG063900 Rh6AG064600 Rh6AG064800 Rh6BG049400 Rh6BG049500 Rh6BG057700 Rh6BG058000 Rh6CG048200 Rh6CG048300 Rh6CG057600 Rh6CG057900 Rh6DG043800 Rh6DG043900
rosa_wichuraiana	Rw2G017640 Rw6G005000 Rw6G005670 Rw6G005710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	1765
AcoI	YGGCCR	1 cut(s)	328
AcsI	RAATTY	3 cut(s)	686, 815, 1771
AcuI	CTGAAG	4 cut(s)	36, 165, 693, 1575
AfaI	GTAC	5 cut(s)	265, 803, 1129, 1445, 1540
AfeI	AGCGCT	1 cut(s)	133
AfiI	CCNNNNNNNGG	2 cut(s)	677, 1740
AgsI	TTSAA	6 cut(s)	340, 1232, 1244, 1388, 1499, 1575
AhlI	ACTAGT	2 cut(s)	307, 1234
AjnI	CCWGG	3 cut(s)	325, 453, 1672
AjuI	GAANNNNNNNTTGG	4 cut(s)	929, 961, 1008, 1040
Alw21I	GWGCWC	2 cut(s)	238, 1785
Alw26I	GTCTC	1 cut(s)	1468
AlwNI	CAGNNNCTG	1 cut(s)	581
Aor51HI	AGCGCT	1 cut(s)	133
AoxI	GGCC	5 cut(s)	154, 328, 948, 1096, 1109
ApeKI	GCWGC	6 cut(s)	164, 176, 374, 435, 548, 1736
ApoI	RAATTY	3 cut(s)	686, 815, 1771
ArsI	GACNNNNNNTTYG	2 cut(s)	1355, 1387
Asp700I	GAANNNNTTC	4 cut(s)	1485, 1636, 1668, 1689
AspLEI	GCGC	2 cut(s)	134, 1517
AspS9I	GGNCC	2 cut(s)	949, 1096
AsuC2I	CCSGG	1 cut(s)	1741
AsuHPI	GGTGA	4 cut(s)	509, 995, 1235, 1723
BaeGI	GKGCMC	1 cut(s)	722
BanII	GRGCYC	1 cut(s)	1785
BauI	CACGAG	1 cut(s)	237
BbsI	GAAGAC	1 cut(s)	47
Bbv12I	GWGCWC	2 cut(s)	238, 1785
BbvI	GCAGC	6 cut(s)	151, 188, 361, 447, 560, 1748
BccI	CCATC	5 cut(s)	147, 532, 804, 973, 1505
BciT130I	CCWGG	3 cut(s)	327, 455, 1674
BcnI	CCSGG	1 cut(s)	1741
BcoDI	GTCTC	1 cut(s)	1468
BcuI	ACTAGT	2 cut(s)	307, 1234
BfaI	CTAG	8 cut(s)	308, 552, 650, 833, 1190, 1235, 1754, 1776
BfmI	CTRYAG	1 cut(s)	375
BfoI	RGCGCY	2 cut(s)	135, 1518
BglI	GCCNNNNNGGC	1 cut(s)	173
BisI	GCNGC	9 cut(s)	165, 177, 331, 375, 433, 436, 549, 605, 1737
BlsI	GCNGC	9 cut(s)	166, 178, 332, 376, 434, 437, 550, 606, 1738
Bme1390I	CCNGG	4 cut(s)	327, 455, 1674, 1741
BmgT120I	GGNCC	2 cut(s)	949, 1096
BmiI	GGNNCC	4 cut(s)	401, 452, 951, 1716
BmrFI	CCNGG	4 cut(s)	327, 455, 1674, 1741
BmrI	ACTGGG	1 cut(s)	725
BmsI	GCATC	6 cut(s)	175, 407, 607, 930, 1028, 1341
BmuI	ACTGGG	1 cut(s)	725
BpiI	GAAGAC	1 cut(s)	47
BplI	GAGNNNNNCTC	2 cut(s)	253, 285
BpmI	CTGGAG	2 cut(s)	476, 884
Bpu10I	CCTNAGC	1 cut(s)	285
BpuEI	CTTGAG	2 cut(s)	280, 1181
BpuMI	CCSGG	1 cut(s)	1741
BsaAI	YACGTR	1 cut(s)	1628
BsaBI	GATNNNNATC	1 cut(s)	1651
BsaXI	ACNNNNNCTCC	2 cut(s)	1037, 1067
Bsc4I	CCNNNNNNNGG	2 cut(s)	677, 1740
Bse118I	RCCGGY	1 cut(s)	828
Bse1I	ACTGG	1 cut(s)	720
Bse8I	GATNNNNATC	1 cut(s)	1651
BseBI	CCWGG	3 cut(s)	327, 455, 1674
BseGI	GGATG	8 cut(s)	139, 568, 754, 793, 965, 1043, 1153, 1516
BseJI	GATNNNNATC	1 cut(s)	1651
BseLI	CCNNNNNNNGG	2 cut(s)	677, 1740
BseMII	CTCAG	3 cut(s)	427, 588, 1657
BseNI	ACTGG	1 cut(s)	720
BseRI	GAGGAG	4 cut(s)	66, 668, 947, 1520
BseSI	GKGCMC	1 cut(s)	722
BseXI	GCAGC	6 cut(s)	151, 188, 361, 447, 560, 1748
BshFI	GGCC	5 cut(s)	156, 330, 950, 1098, 1111
BsiHKAI	GWGCWC	2 cut(s)	238, 1785
BsiSI	CCGG	2 cut(s)	829, 1740
BslFI	GGGAC	1 cut(s)	715
BslI	CCNNNNNNNGG	2 cut(s)	677, 1740
BsmAI	GTCTC	1 cut(s)	1468
BsmFI	GGGAC	1 cut(s)	715
BsnI	GGCC	5 cut(s)	156, 330, 950, 1098, 1111
Bsp1286I	GDGCHC	3 cut(s)	238, 722, 1785
Bsp143I	GATC	2 cut(s)	695, 1072
BspANI	GGCC	5 cut(s)	156, 330, 950, 1098, 1111
BspCNI	CTCAG	3 cut(s)	426, 587, 1656
BspHI	TCATGA	1 cut(s)	1563
BspLI	GGNNCC	4 cut(s)	401, 452, 951, 1716
BspMAI	CTGCAG	1 cut(s)	379
BsrFI	RCCGGY	1 cut(s)	828
BsrI	ACTGG	1 cut(s)	720
BssAI	RCCGGY	1 cut(s)	828
BssMI	GATC	2 cut(s)	695, 1072
BssNAI	GTATAC	1 cut(s)	1766
BssSI	CACGAG	1 cut(s)	237
Bst1107I	GTATAC	1 cut(s)	1766
Bst2BI	CACGAG	1 cut(s)	237
Bst2UI	CCWGG	3 cut(s)	327, 455, 1674
Bst4CI	ACNGT	3 cut(s)	611, 1260, 1392
BstBAI	YACGTR	1 cut(s)	1628
BstC8I	GCNNGC	5 cut(s)	88, 234, 722, 1123, 1330
BstDEI	CTNAG	4 cut(s)	285, 413, 574, 1643
BstENI	CCTNNNNNAGG	1 cut(s)	675
BstF5I	GGATG	8 cut(s)	139, 568, 754, 793, 965, 1043, 1153, 1516
BstH2I	RGCGCY	2 cut(s)	135, 1518
BstHHI	GCGC	2 cut(s)	134, 1517
BstKTI	GATC	2 cut(s)	698, 1075
BstMAI	GTCTC	1 cut(s)	1468
BstMBI	GATC	2 cut(s)	695, 1072
BstMWI	GCNNNNNNNGC	3 cut(s)	173, 185, 1038
BstNI	CCWGG	3 cut(s)	327, 455, 1674
BstNSI	RCATGY	3 cut(s)	90, 1125, 1332
BstSCI	CCNGG	4 cut(s)	325, 453, 1672, 1739
BstSFI	CTRYAG	1 cut(s)	375
BstSLI	GKGCMC	1 cut(s)	722
BstV1I	GCAGC	6 cut(s)	151, 188, 361, 447, 560, 1748
BstV2I	GAAGAC	1 cut(s)	47
BstZ17I	GTATAC	1 cut(s)	1766
BsuRI	GGCC	5 cut(s)	156, 330, 950, 1098, 1111
BtsCI	GGATG	8 cut(s)	139, 568, 754, 793, 965, 1043, 1153, 1516
BtsI	GCAGTG	2 cut(s)	384, 1397
BtsIMutI	CAGTG	4 cut(s)	384, 713, 1397, 1662
Cac8I	GCNNGC	5 cut(s)	88, 234, 722, 1123, 1330
CaiI	CAGNNNCTG	1 cut(s)	581
CciI	TCATGA	1 cut(s)	1563
CfoI	GCGC	2 cut(s)	134, 1517
Cfr10I	RCCGGY	1 cut(s)	828
Cfr13I	GGNCC	2 cut(s)	949, 1096
Csp6I	GTAC	5 cut(s)	264, 802, 1128, 1444, 1539
CspCI	CAANNNNNGTGG	2 cut(s)	507, 542
CviQI	GTAC	5 cut(s)	264, 802, 1128, 1444, 1539
DdeI	CTNAG	4 cut(s)	285, 413, 574, 1643
DpnI	GATC	2 cut(s)	697, 1074
DpnII	GATC	2 cut(s)	695, 1072
EaeI	YGGCCR	1 cut(s)	328
EciI	GGCGGA	1 cut(s)	419
Ecl136II	GAGCTC	1 cut(s)	1783
Eco24I	GRGCYC	1 cut(s)	1785
Eco47III	AGCGCT	1 cut(s)	133
Eco53kI	GAGCTC	1 cut(s)	1783
Eco57I	CTGAAG	4 cut(s)	36, 165, 693, 1575
EcoICRI	GAGCTC	1 cut(s)	1783
EcoNI	CCTNNNNNAGG	1 cut(s)	675
EcoRI	GAATTC	1 cut(s)	1771
EcoRII	CCWGG	3 cut(s)	325, 453, 1672
EcoT22I	ATGCAT	2 cut(s)	190, 1334
EcoT38I	GRGCYC	1 cut(s)	1785
FaqI	GGGAC	1 cut(s)	715
FauI	CCCGC	4 cut(s)	284, 1005, 1034, 1670
FauNDI	CATATG	1 cut(s)	1792
FblI	GTMKAC	1 cut(s)	1765
Fnu4HI	GCNGC	9 cut(s)	165, 177, 331, 375, 433, 436, 549, 605, 1737
FokI	GGATG	8 cut(s)	126, 575, 761, 800, 952, 1050, 1160, 1523
FriOI	GRGCYC	1 cut(s)	1785
Fsp4HI	GCNGC	9 cut(s)	165, 177, 331, 375, 433, 436, 549, 605, 1737
FspBI	CTAG	8 cut(s)	308, 552, 650, 833, 1190, 1235, 1754, 1776
GlaI	GCGC	2 cut(s)	133, 1516
GluI	GCNGC	9 cut(s)	165, 177, 331, 375, 433, 436, 549, 605, 1737
GsuI	CTGGAG	2 cut(s)	476, 884
HaeII	RGCGCY	2 cut(s)	135, 1518
HaeIII	GGCC	5 cut(s)	156, 330, 950, 1098, 1111
HapII	CCGG	2 cut(s)	829, 1740
HhaI	GCGC	2 cut(s)	134, 1517
Hin6I	GCGC	2 cut(s)	132, 1515
HinP1I	GCGC	2 cut(s)	132, 1515
HinfI	GANTC	5 cut(s)	146, 645, 1079, 1244, 1424
HpaII	CCGG	2 cut(s)	829, 1740
HphI	GGTGA	4 cut(s)	509, 995, 1235, 1723
Hpy166II	GTNNAC	2 cut(s)	571, 1766
Hpy188I	TCNGA	8 cut(s)	184, 416, 583, 644, 1253, 1266, 1580, 1646
Hpy188III	TCNNGA	9 cut(s)	110, 143, 239, 407, 1070, 1076, 1361, 1428, 1564
Hpy8I	GTNNAC	2 cut(s)	571, 1766
HpyAV	CCTTC	8 cut(s)	166, 364, 413, 553, 768, 776, 1425, 1679
HpyCH4III	ACNGT	3 cut(s)	611, 1260, 1392
HpyCH4IV	ACGT	3 cut(s)	352, 871, 1627
HpyF10VI	GCNNNNNNNGC	3 cut(s)	173, 185, 1038
HpyF3I	CTNAG	4 cut(s)	285, 413, 574, 1643
HpySE526I	ACGT	3 cut(s)	352, 871, 1627
HspAI	GCGC	2 cut(s)	132, 1515
Kzo9I	GATC	2 cut(s)	695, 1072
LmnI	GCTCC	4 cut(s)	274, 399, 456, 529
Lsp1109I	GCAGC	6 cut(s)	151, 188, 361, 447, 560, 1748
LweI	GCATC	6 cut(s)	175, 407, 607, 930, 1028, 1341
MaeI	CTAG	8 cut(s)	308, 552, 650, 833, 1190, 1235, 1754, 1776
MaeII	ACGT	3 cut(s)	352, 871, 1627
MaeIII	GTNAC	5 cut(s)	30, 67, 310, 1223, 1395
MalI	GATC	2 cut(s)	697, 1074
MboI	GATC	2 cut(s)	695, 1072
MfeI	CAATTG	2 cut(s)	805, 1100
MhlI	GDGCHC	3 cut(s)	238, 722, 1785
MluCI	AATT	6 cut(s)	686, 805, 815, 1100, 1584, 1771
MlyI	GAGTC	1 cut(s)	639
MmeI	TCCRAC	2 cut(s)	296, 1244
Mph1103I	ATGCAT	2 cut(s)	190, 1334
MroXI	GAANNNNTTC	4 cut(s)	1485, 1636, 1668, 1689
MseI	TTAA	4 cut(s)	159, 465, 690, 1649
MslI	CAYNNNNRTG	5 cut(s)	91, 612, 1120, 1348, 1562
MspI	CCGG	2 cut(s)	829, 1740
MspR9I	CCNGG	4 cut(s)	327, 455, 1674, 1741
MunI	CAATTG	2 cut(s)	805, 1100
MvaI	CCWGG	3 cut(s)	327, 455, 1674
MwoI	GCNNNNNNNGC	3 cut(s)	173, 185, 1038
NciI	CCSGG	1 cut(s)	1741
NdeI	CATATG	1 cut(s)	1792
NdeII	GATC	2 cut(s)	695, 1072
NlaIV	GGNNCC	4 cut(s)	401, 452, 951, 1716
NmuCI	GTSAC	3 cut(s)	310, 1223, 1395
NsiI	ATGCAT	2 cut(s)	190, 1334
NspI	RCATGY	3 cut(s)	90, 1125, 1332
PaeI	GCATGC	3 cut(s)	90, 1125, 1332
PagI	TCATGA	1 cut(s)	1563
PdmI	GAANNNNTTC	4 cut(s)	1485, 1636, 1668, 1689
PfeI	GAWTC	4 cut(s)	146, 1079, 1244, 1424
PfoI	TCCNGGA	1 cut(s)	453
PkrI	GCNGC	9 cut(s)	166, 178, 332, 376, 434, 437, 550, 606, 1738
PleI	GAGTC	1 cut(s)	639
PpsI	GAGTC	1 cut(s)	639
Ppu21I	YACGTR	1 cut(s)	1628
Psp124BI	GAGCTC	1 cut(s)	1785
Psp6I	CCWGG	3 cut(s)	325, 453, 1672
PspGI	CCWGG	3 cut(s)	325, 453, 1672
PspN4I	GGNNCC	4 cut(s)	401, 452, 951, 1716
PspPI	GGNCC	2 cut(s)	949, 1096
PstI	CTGCAG	1 cut(s)	379
PstNI	CAGNNNCTG	1 cut(s)	581
RsaI	GTAC	5 cut(s)	265, 803, 1129, 1445, 1540
RsaNI	GTAC	5 cut(s)	264, 802, 1128, 1444, 1539
RseI	CAYNNNNRTG	5 cut(s)	91, 612, 1120, 1348, 1562
SacI	GAGCTC	1 cut(s)	1785
SaqAI	TTAA	4 cut(s)	159, 465, 690, 1649
SatI	GCNGC	9 cut(s)	165, 177, 331, 375, 433, 436, 549, 605, 1737
Sau3AI	GATC	2 cut(s)	695, 1072
Sau96I	GGNCC	2 cut(s)	949, 1096
SchI	GAGTC	1 cut(s)	639
ScrFI	CCNGG	4 cut(s)	327, 455, 1674, 1741
SduI	GDGCHC	3 cut(s)	238, 722, 1785
SfaNI	GCATC	6 cut(s)	175, 407, 607, 930, 1028, 1341
SfcI	CTRYAG	1 cut(s)	375
SmiMI	CAYNNNNRTG	5 cut(s)	91, 612, 1120, 1348, 1562
SmlI	CTYRAG	2 cut(s)	259, 1160
SmoI	CTYRAG	2 cut(s)	259, 1160
SpeI	ACTAGT	2 cut(s)	307, 1234
SphI	GCATGC	3 cut(s)	90, 1125, 1332
Sse9I	AATT	6 cut(s)	686, 805, 815, 1100, 1584, 1771
SspI	AATATT	1 cut(s)	1312
SspMI	CTAG	8 cut(s)	308, 552, 650, 833, 1190, 1235, 1754, 1776
SstI	GAGCTC	1 cut(s)	1785
StyD4I	CCNGG	4 cut(s)	325, 453, 1672, 1739
TaaI	ACNGT	3 cut(s)	611, 1260, 1392
TaiI	ACGT	3 cut(s)	355, 874, 1630
TasI	AATT	6 cut(s)	686, 805, 815, 1100, 1584, 1771
TatI	WGTACW	3 cut(s)	263, 801, 1127
TauI	GCSGC	3 cut(s)	333, 435, 607
TfiI	GAWTC	4 cut(s)	146, 1079, 1244, 1424
Tru1I	TTAA	4 cut(s)	159, 465, 690, 1649
Tru9I	TTAA	4 cut(s)	159, 465, 690, 1649
TscAI	CASTG	4 cut(s)	384, 720, 1404, 1662
TseFI	GTSAC	3 cut(s)	310, 1223, 1395
TseI	GCWGC	6 cut(s)	164, 176, 374, 435, 548, 1736
Tsp45I	GTSAC	3 cut(s)	310, 1223, 1395
TspDTI	ATGAA	7 cut(s)	106, 378, 750, 930, 966, 1319, 1335
TspRI	CASTG	4 cut(s)	384, 720, 1404, 1662
XagI	CCTNNNNNAGG	1 cut(s)	675
XapI	RAATTY	3 cut(s)	686, 815, 1771
XceI	RCATGY	3 cut(s)	90, 1125, 1332
XmiI	GTMKAC	1 cut(s)	1765
XmnI	GAANNNNTTC	4 cut(s)	1485, 1636, 1668, 1689
XspI	CTAG	8 cut(s)	308, 552, 650, 833, 1190, 1235, 1754, 1776
Zsp2I	ATGCAT	2 cut(s)	190, 1334

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.