nuclease HARBI1
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 2 cut(s) | 130, 149 |
| AcuI | CTGAAG | 1 cut(s) | 138 |
| AcyI | GRCGYC | 1 cut(s) | 18 |
| AfaI | GTAC | 1 cut(s) | 435 |
| AgsI | TTSAA | 5 cut(s) | 136, 231, 355, 395, 413 |
| AjiI | CACGTC | 1 cut(s) | 313 |
| AluBI | AGCT | 5 cut(s) | 71, 400, 475, 486, 523 |
| AluI | AGCT | 5 cut(s) | 71, 400, 475, 486, 523 |
| Alw21I | GWGCWC | 1 cut(s) | 477 |
| Alw26I | GTCTC | 3 cut(s) | 241, 273, 326 |
| ApoI | RAATTY | 2 cut(s) | 130, 149 |
| AsuHPI | GGTGA | 1 cut(s) | 467 |
| BanII | GRGCYC | 1 cut(s) | 477 |
| Bbv12I | GWGCWC | 1 cut(s) | 477 |
| BccI | CCATC | 1 cut(s) | 164 |
| BcoDI | GTCTC | 3 cut(s) | 241, 273, 326 |
| BfaI | CTAG | 2 cut(s) | 240, 535 |
| BmgBI | CACGTC | 1 cut(s) | 313 |
| BmsI | GCATC | 1 cut(s) | 247 |
| BpuEI | CTTGAG | 1 cut(s) | 295 |
| BsaHI | GRCGYC | 1 cut(s) | 18 |
| BsaI | GGTCTC | 1 cut(s) | 241 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 196, 226 |
| Bse3DI | GCAATG | 1 cut(s) | 515 |
| BseGI | GGATG | 2 cut(s) | 130, 436 |
| BseMI | GCAATG | 1 cut(s) | 515 |
| BsiHKAI | GWGCWC | 1 cut(s) | 477 |
| BsmAI | GTCTC | 3 cut(s) | 241, 273, 326 |
| Bso31I | GGTCTC | 1 cut(s) | 241 |
| Bsp1286I | GDGCHC | 1 cut(s) | 477 |
| Bsp143I | GATC | 2 cut(s) | 82, 157 |
| BspQI | GCTCTTC | 1 cut(s) | 390 |
| BspTNI | GGTCTC | 1 cut(s) | 241 |
| BsrDI | GCAATG | 1 cut(s) | 515 |
| BssMI | GATC | 2 cut(s) | 82, 157 |
| BssNI | GRCGYC | 1 cut(s) | 18 |
| Bst6I | CTCTTC | 1 cut(s) | 390 |
| BstACI | GRCGYC | 1 cut(s) | 18 |
| BstC8I | GCNNGC | 1 cut(s) | 488 |
| BstDEI | CTNAG | 2 cut(s) | 29, 224 |
| BstF5I | GGATG | 2 cut(s) | 130, 436 |
| BstKTI | GATC | 2 cut(s) | 85, 160 |
| BstMAI | GTCTC | 3 cut(s) | 241, 273, 326 |
| BstMBI | GATC | 2 cut(s) | 82, 157 |
| BstNSI | RCATGY | 2 cut(s) | 116, 365 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 97 |
| BtrI | CACGTC | 1 cut(s) | 313 |
| BtsCI | GGATG | 2 cut(s) | 130, 436 |
| Cac8I | GCNNGC | 1 cut(s) | 488 |
| CseI | GACGC | 1 cut(s) | 26 |
| Csp6I | GTAC | 1 cut(s) | 434 |
| CviAII | CATG | 3 cut(s) | 113, 283, 362 |
| CviJI | RGCY | 8 cut(s) | 71, 89, 104, 287, 400, 475, 486, 523 |
| CviKI_1 | RGCY | 8 cut(s) | 71, 89, 104, 287, 400, 475, 486, 523 |
| CviQI | GTAC | 1 cut(s) | 434 |
| DdeI | CTNAG | 2 cut(s) | 29, 224 |
| DpnI | GATC | 2 cut(s) | 84, 159 |
| DpnII | GATC | 2 cut(s) | 82, 157 |
| DraI | TTTAAA | 1 cut(s) | 501 |
| Eam1104I | CTCTTC | 1 cut(s) | 390 |
| EarI | CTCTTC | 1 cut(s) | 390 |
| Ecl136II | GAGCTC | 1 cut(s) | 475 |
| Eco24I | GRGCYC | 1 cut(s) | 477 |
| Eco31I | GGTCTC | 1 cut(s) | 241 |
| Eco53kI | GAGCTC | 1 cut(s) | 475 |
| Eco57I | CTGAAG | 1 cut(s) | 138 |
| EcoICRI | GAGCTC | 1 cut(s) | 475 |
| EcoT38I | GRGCYC | 1 cut(s) | 477 |
| FaeI | CATG | 3 cut(s) | 116, 286, 365 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 311, 343 |
| FatI | CATG | 3 cut(s) | 112, 282, 361 |
| FokI | GGATG | 2 cut(s) | 137, 443 |
| FriOI | GRGCYC | 1 cut(s) | 477 |
| FspBI | CTAG | 2 cut(s) | 240, 535 |
| HgaI | GACGC | 1 cut(s) | 26 |
| Hin1I | GRCGYC | 1 cut(s) | 18 |
| Hin1II | CATG | 3 cut(s) | 116, 286, 365 |
| HindIII | AAGCTT | 2 cut(s) | 69, 521 |
| HinfI | GANTC | 2 cut(s) | 461, 514 |
| HphI | GGTGA | 1 cut(s) | 467 |
| Hpy166II | GTNNAC | 1 cut(s) | 10 |
| Hpy188I | TCNGA | 1 cut(s) | 237 |
| Hpy188III | TCNNGA | 1 cut(s) | 161 |
| Hpy8I | GTNNAC | 1 cut(s) | 10 |
| HpyAV | CCTTC | 3 cut(s) | 158, 225, 407 |
| HpyCH4IV | ACGT | 1 cut(s) | 312 |
| HpyCH4V | TGCA | 3 cut(s) | 4, 365, 443 |
| HpyF3I | CTNAG | 2 cut(s) | 29, 224 |
| HpySE526I | ACGT | 1 cut(s) | 312 |
| Hsp92I | GRCGYC | 1 cut(s) | 18 |
| Hsp92II | CATG | 3 cut(s) | 116, 286, 365 |
| Kzo9I | GATC | 2 cut(s) | 82, 157 |
| LguI | GCTCTTC | 1 cut(s) | 390 |
| LmnI | GCTCC | 1 cut(s) | 305 |
| LpnPI | CCDG | 2 cut(s) | 265, 504 |
| LweI | GCATC | 1 cut(s) | 247 |
| MaeI | CTAG | 2 cut(s) | 240, 535 |
| MaeII | ACGT | 1 cut(s) | 312 |
| MaeIII | GTNAC | 1 cut(s) | 313 |
| MalI | GATC | 2 cut(s) | 84, 159 |
| MboI | GATC | 2 cut(s) | 82, 157 |
| MboII | GAAGA | 1 cut(s) | 407 |
| MhlI | GDGCHC | 1 cut(s) | 477 |
| MluCI | AATT | 5 cut(s) | 130, 149, 447, 468, 496 |
| MlyI | GAGTC | 1 cut(s) | 455 |
| MnlI | CCTC | 1 cut(s) | 498 |
| MseI | TTAA | 3 cut(s) | 36, 500, 528 |
| NdeII | GATC | 2 cut(s) | 82, 157 |
| NlaIII | CATG | 3 cut(s) | 116, 286, 365 |
| NmuCI | GTSAC | 1 cut(s) | 313 |
| NspI | RCATGY | 2 cut(s) | 116, 365 |
| PciSI | GCTCTTC | 1 cut(s) | 390 |
| PfeI | GAWTC | 1 cut(s) | 514 |
| PleI | GAGTC | 1 cut(s) | 455 |
| PpsI | GAGTC | 1 cut(s) | 455 |
| Psp124BI | GAGCTC | 1 cut(s) | 477 |
| RsaI | GTAC | 1 cut(s) | 435 |
| RsaNI | GTAC | 1 cut(s) | 434 |
| SacI | GAGCTC | 1 cut(s) | 477 |
| SapI | GCTCTTC | 1 cut(s) | 390 |
| SaqAI | TTAA | 3 cut(s) | 36, 500, 528 |
| Sau3AI | GATC | 2 cut(s) | 82, 157 |
| SchI | GAGTC | 1 cut(s) | 455 |
| SduI | GDGCHC | 1 cut(s) | 477 |
| SetI | ASST | 7 cut(s) | 73, 315, 402, 418, 477, 488, 525 |
| SfaNI | GCATC | 1 cut(s) | 247 |
| SmlI | CTYRAG | 1 cut(s) | 274 |
| SmoI | CTYRAG | 1 cut(s) | 274 |
| Sse9I | AATT | 5 cut(s) | 130, 149, 447, 468, 496 |
| SspMI | CTAG | 2 cut(s) | 240, 535 |
| SstI | GAGCTC | 1 cut(s) | 477 |
| TaiI | ACGT | 1 cut(s) | 315 |
| TaqI | TCGA | 2 cut(s) | 85, 162 |
| TasI | AATT | 5 cut(s) | 130, 149, 447, 468, 496 |
| TatI | WGTACW | 1 cut(s) | 433 |
| TfiI | GAWTC | 1 cut(s) | 514 |
| Tru1I | TTAA | 3 cut(s) | 36, 500, 528 |
| Tru9I | TTAA | 3 cut(s) | 36, 500, 528 |
| TseFI | GTSAC | 1 cut(s) | 313 |
| Tsp45I | GTSAC | 1 cut(s) | 313 |
| TspDTI | ATGAA | 7 cut(s) | 34, 38, 141, 372, 419, 434, 506 |
| XapI | RAATTY | 2 cut(s) | 130, 149 |
| XceI | RCATGY | 2 cut(s) | 116, 365 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 325 |
| XspI | CTAG | 2 cut(s) | 240, 535 |