Rmu_sc0006651.1_g000003
MYB Family

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006651.1
OG0001154
Physical Location & Seq
Forward (+)
10175 .. 11409
1235 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006651.1_g000003.1.cds

Sequence Viewer

Length: 876 bp
atggatttgcaagcaaactcaaggttctcaactagtagaagtggaagaaataaagagaagcaaccattacacacctggacccctattgaagatgtagctctagttgcggctttgactgaacttcgtgtttctgggagctggaaggttgacaatggattcaaggcaggatatttgggacaactagaaaaaattatagagcaaaagctaccaaattgtggattaaaggtgaatccccacgttgagtcttgtgttaagacactaaagaaacaaacgcttgccatctctgacatgattgctaactcaagtggcttctcatgggaccatgaaaataaaatggttcacaaggaagccaaaggactaagacttaaaccattcattcattatgatagtttggtggaagcttttgggagagatagaaccaatgggttaggagctgaaggacatgctgaagcgaatgaagatttgaataatgataataaatttgttgatcttgaagaagatggtttgcctgaaaatattgttcctacacctgctactcgtacaacttcttatgcttctaatgcaactcggactagaaagcgagcaagggacgcttatgctcaatacttgggagacatggctagcacacttcgcttctttgtggatgtgaccaagacacacttggagactatgaaagaagtacttttaaatgaccatgcaacatctacaaaaagaggtaagcttgttgaagagcttatgaaaattgaaggtttgaatgattatgatgtgcttgaggctgttgctacaattattggcgatgattccaaaattgagctcttgttcagcttacctgacaatttaaagacccaatggatttacaagcttcttaaccactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4 Myb_DNA-bind_3

Protein Analysis

291

Amino Acids

32.69

Weight (kDa)

6.16

Isoelectric Point (pI)

39.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 538
Acc36I ACCTGC 1 cut(s) 538
AccB7I CCANNNNNTGG 1 cut(s) 215
AciI CCGC 1 cut(s) 107
AcsI RAATTY 1 cut(s) 479
AcuI CTGAAG 2 cut(s) 456, 468
AfaI GTAC 2 cut(s) 541, 681
AfiI CCNNNNNNNGG 1 cut(s) 215
AgsI TTSAA 7 cut(s) 89, 160, 466, 494, 728, 746, 754
AhlI ACTAGT 1 cut(s) 32
AjnI CCWGG 1 cut(s) 74
Alw21I GWGCWC 1 cut(s) 816
Alw26I GTCTC 2 cut(s) 606, 659
ApoI RAATTY 1 cut(s) 479
AspS9I GGNCC 2 cut(s) 78, 319
AsuHPI GGTGA 1 cut(s) 238
AsuNHI GCTAGC 1 cut(s) 620
AvaII GGWCC 2 cut(s) 78, 319
BanII GRGCYC 1 cut(s) 816
Bbv12I GWGCWC 1 cut(s) 816
BccI CCATC 2 cut(s) 287, 494
BciT130I CCWGG 1 cut(s) 76
BcoDI GTCTC 2 cut(s) 606, 659
BcuI ACTAGT 1 cut(s) 32
BfaI CTAG 6 cut(s) 33, 101, 182, 573, 621, 874
BfuAI ACCTGC 1 cut(s) 538
BisI GCNGC 1 cut(s) 108
BlsI GCNGC 1 cut(s) 109
BmcAI AGTACT 1 cut(s) 681
Bme1390I CCNGG 1 cut(s) 76
Bme18I GGWCC 2 cut(s) 78, 319
BmgT120I GGNCC 2 cut(s) 78, 319
BmiI GGNNCC 2 cut(s) 80, 320
BmrFI CCNGG 1 cut(s) 76
BmtI GCTAGC 1 cut(s) 624
BpuEI CTTGAG 3 cut(s) 4, 286, 791
Bsc4I CCNNNNNNNGG 1 cut(s) 215
BseBI CCWGG 1 cut(s) 76
BseGI GGATG 1 cut(s) 649
BseLI CCNNNNNNNGG 1 cut(s) 215
BsiHKAI GWGCWC 1 cut(s) 816
BslFI GGGAC 3 cut(s) 189, 332, 602
BslI CCNNNNNNNGG 1 cut(s) 215
BsmAI GTCTC 2 cut(s) 606, 659
BsmFI GGGAC 3 cut(s) 189, 332, 602
Bsp1286I GDGCHC 1 cut(s) 816
Bsp143I GATC 1 cut(s) 487
BspACI CCGC 1 cut(s) 107
BspLI GGNNCC 2 cut(s) 80, 320
BspMI ACCTGC 1 cut(s) 538
BspOI GCTAGC 1 cut(s) 624
BspQI GCTCTTC 1 cut(s) 723
BssMI GATC 1 cut(s) 487
Bst2UI CCWGG 1 cut(s) 76
Bst6I CTCTTC 1 cut(s) 723
BstC8I GCNNGC 4 cut(s) 12, 276, 582, 622
BstDEI CTNAG 1 cut(s) 359
BstF5I GGATG 1 cut(s) 649
BstKTI GATC 1 cut(s) 490
BstMAI GTCTC 2 cut(s) 606, 659
BstMBI GATC 1 cut(s) 487
BstMWI GCNNNNNNNGC 4 cut(s) 104, 560, 590, 630
BstNI CCWGG 1 cut(s) 76
BstNSI RCATGY 1 cut(s) 446
BstSCI CCNGG 1 cut(s) 74
BtgZI GCGATG 1 cut(s) 810
BtsCI GGATG 1 cut(s) 649
BveI ACCTGC 1 cut(s) 538
Cac8I GCNNGC 4 cut(s) 12, 276, 582, 622
Cfr13I GGNCC 2 cut(s) 78, 319
CseI GACGC 1 cut(s) 599
Csp6I GTAC 2 cut(s) 540, 680
CviAII CATG 6 cut(s) 289, 315, 323, 443, 616, 695
CviQI GTAC 2 cut(s) 540, 680
DdeI CTNAG 1 cut(s) 359
DpnI GATC 1 cut(s) 489
DpnII GATC 1 cut(s) 487
DraI TTTAAA 2 cut(s) 687, 840
Eam1104I CTCTTC 1 cut(s) 723
EarI CTCTTC 1 cut(s) 723
Ecl136II GAGCTC 1 cut(s) 814
Eco24I GRGCYC 1 cut(s) 816
Eco47I GGWCC 2 cut(s) 78, 319
Eco53kI GAGCTC 1 cut(s) 814
Eco57I CTGAAG 2 cut(s) 456, 468
EcoICRI GAGCTC 1 cut(s) 814
EcoRII CCWGG 1 cut(s) 74
EcoT38I GRGCYC 1 cut(s) 816
FaeI CATG 6 cut(s) 292, 318, 326, 446, 619, 698
FalI AAGNNNNNCTT 6 cut(s) 577, 609, 644, 676, 666, 698
FaqI GGGAC 3 cut(s) 189, 332, 602
FatI CATG 6 cut(s) 288, 314, 322, 442, 615, 694
Fnu4HI GCNGC 1 cut(s) 108
FokI GGATG 1 cut(s) 656
FriOI GRGCYC 1 cut(s) 816
Fsp4HI GCNGC 1 cut(s) 108
FspBI CTAG 6 cut(s) 33, 101, 182, 573, 621, 874
GluI GCNGC 1 cut(s) 108
HgaI GACGC 1 cut(s) 599
Hin1II CATG 6 cut(s) 292, 318, 326, 446, 619, 698
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HindIII AAGCTT 3 cut(s) 399, 719, 860
HinfI GANTC 4 cut(s) 156, 229, 242, 800
HphI GGTGA 1 cut(s) 238
Hpy166II GTNNAC 2 cut(s) 148, 340
Hpy188I TCNGA 2 cut(s) 286, 570
Hpy188III TCNNGA 1 cut(s) 491
Hpy8I GTNNAC 2 cut(s) 148, 340
HpyAV CCTTC 3 cut(s) 136, 431, 740
HpyCH4IV ACGT 1 cut(s) 237
HpyCH4V TGCA 3 cut(s) 10, 563, 698
HpyF10VI GCNNNNNNNGC 4 cut(s) 104, 560, 590, 630
HpyF3I CTNAG 1 cut(s) 359
HpySE526I ACGT 1 cut(s) 237
Hsp92II CATG 6 cut(s) 292, 318, 326, 446, 619, 698
Kzo9I GATC 1 cut(s) 487
LguI GCTCTTC 1 cut(s) 723
LmnI GCTCC 2 cut(s) 135, 431
LpnPI CCDG 8 cut(s) 61, 88, 117, 124, 150, 522, 543, 843
MaeI CTAG 6 cut(s) 33, 101, 182, 573, 621, 874
MaeII ACGT 1 cut(s) 237
MaeIII GTNAC 1 cut(s) 646
MalI GATC 1 cut(s) 489
MboI GATC 1 cut(s) 487
MboII GAAGA 6 cut(s) 57, 101, 470, 506, 509, 740
MhlI GDGCHC 1 cut(s) 816
MluCI AATT 7 cut(s) 189, 211, 479, 741, 786, 807, 835
MlyI GAGTC 1 cut(s) 251
MnlI CCTC 2 cut(s) 707, 766
MseI TTAA 6 cut(s) 221, 252, 366, 686, 839, 867
MspR9I CCNGG 1 cut(s) 76
MvaI CCWGG 1 cut(s) 76
MwoI GCNNNNNNNGC 4 cut(s) 104, 560, 590, 630
NdeII GATC 1 cut(s) 487
NheI GCTAGC 1 cut(s) 620
NlaIII CATG 6 cut(s) 292, 318, 326, 446, 619, 698
NlaIV GGNNCC 2 cut(s) 80, 320
NmuCI GTSAC 1 cut(s) 646
NspI RCATGY 1 cut(s) 446
PaqCI CACCTGC 1 cut(s) 538
PciSI GCTCTTC 1 cut(s) 723
PfeI GAWTC 3 cut(s) 156, 229, 800
PflMI CCANNNNNTGG 1 cut(s) 215
PkrI GCNGC 1 cut(s) 109
PleI GAGTC 1 cut(s) 250
PpsI GAGTC 1 cut(s) 250
Psp124BI GAGCTC 1 cut(s) 816
Psp6I CCWGG 1 cut(s) 74
PspGI CCWGG 1 cut(s) 74
PspN4I GGNNCC 2 cut(s) 80, 320
PspPI GGNCC 2 cut(s) 78, 319
RsaI GTAC 2 cut(s) 541, 681
RsaNI GTAC 2 cut(s) 540, 680
SacI GAGCTC 1 cut(s) 816
SapI GCTCTTC 1 cut(s) 723
SaqAI TTAA 6 cut(s) 221, 252, 366, 686, 839, 867
SatI GCNGC 1 cut(s) 108
Sau3AI GATC 1 cut(s) 487
Sau96I GGNCC 2 cut(s) 78, 319
ScaI AGTACT 1 cut(s) 681
SchI GAGTC 1 cut(s) 251
ScrFI CCNGG 1 cut(s) 76
SduI GDGCHC 1 cut(s) 816
SinI GGWCC 2 cut(s) 78, 319
SmlI CTYRAG 3 cut(s) 19, 301, 770
SmoI CTYRAG 3 cut(s) 19, 301, 770
SpeI ACTAGT 1 cut(s) 32
Sse9I AATT 7 cut(s) 189, 211, 479, 741, 786, 807, 835
SsiI CCGC 1 cut(s) 107
SspI AATATT 1 cut(s) 517
SspMI CTAG 6 cut(s) 33, 101, 182, 573, 621, 874
SstI GAGCTC 1 cut(s) 816
StyD4I CCNGG 1 cut(s) 74
TaiI ACGT 1 cut(s) 240
TasI AATT 7 cut(s) 189, 211, 479, 741, 786, 807, 835
TatI WGTACW 1 cut(s) 679
TauI GCSGC 1 cut(s) 110
TfiI GAWTC 3 cut(s) 156, 229, 800
Tru1I TTAA 6 cut(s) 221, 252, 366, 686, 839, 867
Tru9I TTAA 6 cut(s) 221, 252, 366, 686, 839, 867
TseFI GTSAC 1 cut(s) 646
Tsp45I GTSAC 1 cut(s) 646
TspDTI ATGAA 6 cut(s) 339, 364, 368, 471, 686, 752
Van91I CCANNNNNTGG 1 cut(s) 215
VpaK11BI GGWCC 2 cut(s) 78, 319
XapI RAATTY 1 cut(s) 479
XceI RCATGY 1 cut(s) 446
XcmI CCANNNNNNNNNTGG 2 cut(s) 72, 658
XspI CTAG 6 cut(s) 33, 101, 182, 573, 621, 874
ZrmI AGTACT 1 cut(s) 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.