Rorug02G0052000
MYB Family

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001154
Physical Location & Seq
Forward (+)
3984639 .. 3987555
2917 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0052000.1

Sequence Viewer

Length: 1383 bp
ATGGAGGTTGCAAGGGCTGACCGAGCTGACGTGGCAGAGGGAGATAAGGAAGACGTGGATTTGGCCGTGAAAGCTGCACGCGCCGCCTTTGACGGTGGCCCCTGGCCTCGCTTGCCCGGCGTTGAGAGGAGAAAGATAATGATGAAGTTTGCGGACTTGATCGATGAACACACAGAAGAACTAGCCAAGTTGGATACAGTTGATGCGGGGAAGTTGTTCAGTTATGGCAAAGCCGCTGACATACCTCAGGTATCACAAACTATACGTTACTATGCGGGTGCAGCTGATAAAATTCATGGAGAGGTGCTCAAAATGTCGCGCGAGCTTCAAGCTTATACTTTACTTGAACCAATTGGTGTTCTGGGACACATCATTCCATGGAATTTTCCGAGCGGCATGTTCTCTGTTAAAGTTGCTCCTTCCTTAGCTGCTGGTTGCACCATGGTCATCAAACCTGCTGAGCAGACACCTCTTTCAGCTCTCTATTATGCTCATCTGGCCAAGTTGGCTGGTGTTCCTGATGGAGTGATTAATGTCATAACTGGATATGGAGAGACTGCTGGTGCGGCCATCACTCATCATATGGACATTGACAAAGTCAGTTTTACTGGTTCCACAGACGTAGGCCGTCAAGTGATGCAGGCTGCAGCAAAGAGCAACTTAAAAGCAGTTTCACTTGAACTAGGGGGCAAGTCACCCCTTTTAATTTTTGATGATGCTGATGTAGATAAGGCTGCTGACCTTGCTCTCTTGGGAATCCTCTACAACAAGGGAGAAACTTGTGTGGCAAGTTCTCGTGTTTTTGTTCAAGAAGGGATCTATGATGAACTTGTGAAGAAATTAGAAGTAAAGGCAAAATCTTGGGCTGTTGGGGATCCTTTTGATCCATATGTTCGTCAAAGACCCCAGGTTGATAAGCAGCAGTTTGAGAAAGTCTTAACCTACATTGAGCATGGAAAAAGAGAAGGAGCCACTTTGTTAACAGGGGGCAAGCCTTTGGGGAACAAGGGATATTACATCGAGCCAACAGTATTCACTGATGTCAAGGATGACATGCTCATAGCCAAGGATGAGATATTTGGACCTGTAATGTCACTGATGAAGTTCAAGACCTTAGAGGAGGCAATACAGAGAGCCAACAACACCAAATATGGCCTAGCAGCAGGCATTGTAACCAAGAACATCGACATTGCTAACACCGTCTCAAGATCAATCCGTGCCGGTAGTATATGGATCAACTGCTACTTCGCCATCGACAACGATTGCCCTTTTGGAGGATACAAGATGAGTGGTTTTGGAAAAGACTGGGGTATGCAGGGCATCCACAAGTATATGCATACTAAATCTGTGGTCACTCCCATCTATAACTCTCCATGGCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4 Myb_DNA-bind_3

Protein Analysis

460

Amino Acids

50.22

Weight (kDa)

6.18

Isoelectric Point (pI)

29.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldedh PF00171 9 - 450 2.3e-167 Aldehyde dehydrogenase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 463
AccBSI CCGCTC 1 cut(s) 393
AccII CGCG 3 cut(s) 81, 319, 321
AciI CCGC 7 cut(s) 84, 152, 206, 234, 275, 393, 566
AclWI GGATC 5 cut(s) 824, 869, 878, 882, 1241
AcoI YGGCCR 3 cut(s) 63, 498, 567
AcsI RAATTY 2 cut(s) 291, 382
AfiI CCNNNNNNNGG 1 cut(s) 1274
AgsI TTSAA 5 cut(s) 329, 347, 680, 809, 1108
AjiI CACGTC 2 cut(s) 31, 55
AjnI CCWGG 2 cut(s) 101, 906
AluBI AGCT 7 cut(s) 26, 74, 284, 325, 332, 428, 479
AluI AGCT 7 cut(s) 26, 74, 284, 325, 332, 428, 479
Alw21I GWGCWC 1 cut(s) 309
Alw26I GTCTC 2 cut(s) 548, 1207
AlwI GGATC 5 cut(s) 824, 869, 878, 882, 1241
AoxI GGCC 7 cut(s) 63, 97, 104, 498, 567, 625, 1153
ApeKI GCWGC 8 cut(s) 74, 281, 428, 644, 647, 734, 919, 1160
ApoI RAATTY 2 cut(s) 291, 382
AseI ATTAAT 1 cut(s) 531
Asp700I GAANNNNTTC 1 cut(s) 215
AspLEI GCGC 2 cut(s) 83, 321
AspS9I GGNCC 2 cut(s) 98, 1082
AsuC2I CCSGG 1 cut(s) 117
AsuHPI GGTGA 1 cut(s) 687
AvaII GGWCC 1 cut(s) 1082
AxyI CCTNAGG 1 cut(s) 246
BalI TGGCCA 1 cut(s) 500
BamHI GGATCC 1 cut(s) 874
BauI CACGAG 1 cut(s) 795
BbsI GAAGAC 1 cut(s) 57
Bbv12I GWGCWC 1 cut(s) 309
BbvI GCAGC 8 cut(s) 61, 293, 415, 631, 659, 721, 931, 1172
BccI CCATC 4 cut(s) 515, 578, 1259, 1367
BceAI ACGGC 2 cut(s) 50, 612
BciT130I CCWGG 2 cut(s) 103, 908
BciVI GTATCC 2 cut(s) 187, 1271
BcnI CCSGG 1 cut(s) 117
BcoDI GTCTC 2 cut(s) 548, 1207
BfaI CTAG 3 cut(s) 182, 683, 1157
BfmI CTRYAG 1 cut(s) 645
BfuAI ACCTGC 1 cut(s) 463
BfuI GTATCC 2 cut(s) 187, 1271
BglI GCCNNNNNGGC 1 cut(s) 506
BlpI GCTNAGC 1 cut(s) 459
Bme1390I CCNGG 3 cut(s) 103, 117, 908
Bme18I GGWCC 1 cut(s) 1082
BmgBI CACGTC 2 cut(s) 31, 55
BmgT120I GGNCC 2 cut(s) 98, 1082
BmiI GGNNCC 4 cut(s) 100, 613, 876, 970
BmrFI CCNGG 3 cut(s) 103, 117, 908
BmrI ACTGGG 1 cut(s) 1315
BmsI GCATC 4 cut(s) 193, 627, 706, 1329
BmuI ACTGGG 1 cut(s) 1315
BpiI GAAGAC 1 cut(s) 57
BplI GAGNNNNNCTC 2 cut(s) 291, 323
Bpu10I CCTNAGC 1 cut(s) 424
Bpu1102I GCTNAGC 1 cut(s) 459
BpuEI CTTGAG 1 cut(s) 1189
BpuMI CCSGG 1 cut(s) 117
Bsa29I ATCGAT 1 cut(s) 162
BsaJI CCNNGG 6 cut(s) 101, 377, 441, 906, 1065, 1373
Bsc4I CCNNNNNNNGG 1 cut(s) 1274
Bse118I RCCGGY 1 cut(s) 1220
Bse1I ACTGG 3 cut(s) 547, 613, 1310
Bse21I CCTNAGG 1 cut(s) 246
Bse3DI GCAATG 1 cut(s) 1188
BseBI CCWGG 2 cut(s) 103, 908
BseCI ATCGAT 1 cut(s) 162
BseDI CCNNGG 6 cut(s) 101, 377, 441, 906, 1065, 1373
BseGI GGATG 3 cut(s) 1054, 1075, 1320
BseLI CCNNNNNNNGG 1 cut(s) 1274
BseMI GCAATG 1 cut(s) 1188
BseMII CTCAG 2 cut(s) 260, 450
BseNI ACTGG 3 cut(s) 547, 613, 1310
BseRI GAGGAG 2 cut(s) 142, 1133
BseXI GCAGC 8 cut(s) 61, 293, 415, 631, 659, 721, 931, 1172
BsgI GTGCAG 2 cut(s) 60, 300
Bsh1236I CGCG 3 cut(s) 81, 319, 321
BshFI GGCC 7 cut(s) 65, 99, 106, 500, 569, 627, 1155
BshVI ATCGAT 1 cut(s) 162
BsiHKAI GWGCWC 1 cut(s) 309
BsiSI CCGG 2 cut(s) 117, 1221
BslFI GGGAC 1 cut(s) 378
BslI CCNNNNNNNGG 1 cut(s) 1274
BsmAI GTCTC 2 cut(s) 548, 1207
BsmBI CGTCTC 1 cut(s) 1207
BsmFI GGGAC 1 cut(s) 378
BsnI GGCC 7 cut(s) 65, 99, 106, 500, 569, 627, 1155
Bsp1286I GDGCHC 1 cut(s) 309
Bsp143I GATC 6 cut(s) 159, 816, 874, 883, 1208, 1233
Bsp1720I GCTNAGC 1 cut(s) 459
Bsp19I CCATGG 3 cut(s) 377, 441, 1373
BspACI CCGC 7 cut(s) 84, 152, 206, 234, 275, 393, 566
BspANI GGCC 7 cut(s) 65, 99, 106, 500, 569, 627, 1155
BspCNI CTCAG 2 cut(s) 259, 451
BspDI ATCGAT 1 cut(s) 162
BspFNI CGCG 3 cut(s) 81, 319, 321
BspLI GGNNCC 4 cut(s) 100, 613, 876, 970
BspMAI CTGCAG 1 cut(s) 649
BspMI ACCTGC 1 cut(s) 463
BspPI GGATC 5 cut(s) 824, 869, 878, 882, 1241
BsrBI CCGCTC 1 cut(s) 393
BsrDI GCAATG 1 cut(s) 1188
BsrFI RCCGGY 1 cut(s) 1220
BsrI ACTGG 3 cut(s) 547, 613, 1310
BssAI RCCGGY 1 cut(s) 1220
BssECI CCNNGG 6 cut(s) 101, 377, 441, 906, 1065, 1373
BssMI GATC 6 cut(s) 159, 816, 874, 883, 1208, 1233
BssSI CACGAG 1 cut(s) 795
BssT1I CCWWGG 4 cut(s) 377, 441, 1065, 1373
Bst2BI CACGAG 1 cut(s) 795
Bst2UI CCWGG 2 cut(s) 103, 908
Bst4CI ACNGT 4 cut(s) 95, 199, 1030, 1201
BstC8I GCNNGC 6 cut(s) 79, 113, 323, 642, 992, 1165
BstDEI CTNAG 4 cut(s) 246, 424, 459, 1114
BstDSI CCRYGG 3 cut(s) 377, 441, 1373
BstENI CCTNNNNNAGG 1 cut(s) 1272
BstF5I GGATG 3 cut(s) 1054, 1075, 1320
BstFNI CGCG 3 cut(s) 81, 319, 321
BstHHI GCGC 2 cut(s) 83, 321
BstKTI GATC 6 cut(s) 162, 819, 877, 886, 1211, 1236
BstMAI GTCTC 2 cut(s) 548, 1207
BstMBI GATC 6 cut(s) 159, 816, 874, 883, 1208, 1233
BstNI CCWGG 2 cut(s) 103, 908
BstNSI RCATGY 2 cut(s) 400, 1057
BstSCI CCNGG 3 cut(s) 101, 115, 906
BstSFI CTRYAG 1 cut(s) 645
BstUI CGCG 3 cut(s) 81, 319, 321
BstV1I GCAGC 8 cut(s) 61, 293, 415, 631, 659, 721, 931, 1172
BstV2I GAAGAC 1 cut(s) 57
BstX2I RGATCY 2 cut(s) 816, 874
BstYI RGATCY 2 cut(s) 816, 874
Bsu15I ATCGAT 1 cut(s) 162
Bsu36I CCTNAGG 1 cut(s) 246
BsuI GTATCC 2 cut(s) 187, 1271
BsuRI GGCC 7 cut(s) 65, 99, 106, 500, 569, 627, 1155
BsuTUI ATCGAT 1 cut(s) 162
BtgI CCRYGG 3 cut(s) 377, 441, 1373
BtrI CACGTC 2 cut(s) 31, 55
BtsCI GGATG 3 cut(s) 1054, 1075, 1320
BtsIMutI CAGTG 2 cut(s) 1035, 1094
BveI ACCTGC 1 cut(s) 463
Cac8I GCNNGC 6 cut(s) 79, 113, 323, 642, 992, 1165
CfoI GCGC 2 cut(s) 83, 321
Cfr10I RCCGGY 1 cut(s) 1220
Cfr13I GGNCC 2 cut(s) 98, 1082
ClaI ATCGAT 1 cut(s) 162
CspCI CAANNNNNGTGG 2 cut(s) 1270, 1305
CviAII CATG 7 cut(s) 296, 378, 397, 442, 953, 1054, 1374
DdeI CTNAG 4 cut(s) 246, 424, 459, 1114
DpnI GATC 6 cut(s) 161, 818, 876, 885, 1210, 1235
DpnII GATC 6 cut(s) 159, 816, 874, 883, 1208, 1233
EaeI YGGCCR 3 cut(s) 63, 498, 567
Eco130I CCWWGG 4 cut(s) 377, 441, 1065, 1373
Eco47I GGWCC 1 cut(s) 1082
Eco81I CCTNAGG 1 cut(s) 246
EcoNI CCTNNNNNAGG 1 cut(s) 1272
EcoRII CCWGG 2 cut(s) 101, 906
EcoT14I CCWWGG 4 cut(s) 377, 441, 1065, 1373
EcoT22I ATGCAT 1 cut(s) 1338
ErhI CCWWGG 4 cut(s) 377, 441, 1065, 1373
Esp3I CGTCTC 1 cut(s) 1207
FaeI CATG 7 cut(s) 299, 381, 400, 445, 956, 1057, 1377
FalI AAGNNNNNCTT 2 cut(s) 644, 676
FaqI GGGAC 1 cut(s) 378
FatI CATG 7 cut(s) 295, 377, 396, 441, 952, 1053, 1373
FauI CCCGC 2 cut(s) 199, 268
FauNDI CATATG 2 cut(s) 582, 889
FokI GGATG 3 cut(s) 1061, 1082, 1307
FspBI CTAG 3 cut(s) 182, 683, 1157
GlaI GCGC 2 cut(s) 82, 320
HaeIII GGCC 7 cut(s) 65, 99, 106, 500, 569, 627, 1155
HapII CCGG 2 cut(s) 117, 1221
HhaI GCGC 2 cut(s) 83, 321
Hin1II CATG 7 cut(s) 299, 381, 400, 445, 956, 1057, 1377
Hin6I GCGC 2 cut(s) 81, 319
HinP1I GCGC 2 cut(s) 81, 319
HincII GTYRAC 1 cut(s) 981
HindII GTYRAC 1 cut(s) 981
HindIII AAGCTT 1 cut(s) 330
HinfI GANTC 1 cut(s) 756
HpaI GTTAAC 1 cut(s) 981
HpaII CCGG 2 cut(s) 117, 1221
HphI GGTGA 1 cut(s) 687
Hpy166II GTNNAC 1 cut(s) 981
Hpy188I TCNGA 1 cut(s) 390
Hpy188III TCNNGA 4 cut(s) 518, 809, 1108, 1206
Hpy8I GTNNAC 1 cut(s) 981
HpyAV CCTTC 3 cut(s) 429, 806, 959
HpyCH4III ACNGT 4 cut(s) 95, 199, 1030, 1201
HpyCH4IV ACGT 4 cut(s) 30, 54, 265, 621
HpyCH4V TGCA 8 cut(s) 11, 77, 281, 438, 640, 647, 1315, 1336
HpyF3I CTNAG 4 cut(s) 246, 424, 459, 1114
HpySE526I ACGT 4 cut(s) 30, 54, 265, 621
Hsp92II CATG 7 cut(s) 299, 381, 400, 445, 956, 1057, 1377
HspAI GCGC 2 cut(s) 81, 319
KspAI GTTAAC 1 cut(s) 981
Kzo9I GATC 6 cut(s) 159, 816, 874, 883, 1208, 1233
LmnI GCTCC 2 cut(s) 421, 968
Lsp1109I GCAGC 8 cut(s) 61, 293, 415, 631, 659, 721, 931, 1172
LweI GCATC 4 cut(s) 193, 627, 706, 1329
MaeI CTAG 3 cut(s) 182, 683, 1157
MaeII ACGT 4 cut(s) 30, 54, 265, 621
MaeIII GTNAC 5 cut(s) 266, 693, 1092, 1171, 1351
MalI GATC 6 cut(s) 161, 818, 876, 885, 1210, 1235
MbiI CCGCTC 1 cut(s) 393
MboI GATC 6 cut(s) 159, 816, 874, 883, 1208, 1233
MboII GAAGA 3 cut(s) 62, 188, 847
MfeI CAATTG 1 cut(s) 351
MflI RGATCY 2 cut(s) 816, 874
MhlI GDGCHC 1 cut(s) 309
MlsI TGGCCA 1 cut(s) 500
MluCI AATT 5 cut(s) 291, 351, 382, 705, 839
MluNI TGGCCA 1 cut(s) 500
MmeI TCCRAC 1 cut(s) 171
Mox20I TGGCCA 1 cut(s) 500
Mph1103I ATGCAT 1 cut(s) 1338
MroXI GAANNNNTTC 1 cut(s) 215
MscI TGGCCA 1 cut(s) 500
MseI TTAA 6 cut(s) 408, 531, 662, 704, 938, 980
Msp20I TGGCCA 1 cut(s) 500
MspA1I CMGCKG 2 cut(s) 236, 284
MspI CCGG 2 cut(s) 117, 1221
MspR9I CCNGG 3 cut(s) 103, 117, 908
MunI CAATTG 1 cut(s) 351
MvaI CCWGG 2 cut(s) 103, 908
MvnI CGCG 3 cut(s) 81, 319, 321
NciI CCSGG 1 cut(s) 117
NcoI CCATGG 3 cut(s) 377, 441, 1373
NdeI CATATG 2 cut(s) 582, 889
NdeII GATC 6 cut(s) 159, 816, 874, 883, 1208, 1233
NlaIII CATG 7 cut(s) 299, 381, 400, 445, 956, 1057, 1377
NlaIV GGNNCC 4 cut(s) 100, 613, 876, 970
NmuCI GTSAC 3 cut(s) 693, 1092, 1351
NsiI ATGCAT 1 cut(s) 1338
NspI RCATGY 2 cut(s) 400, 1057
PdmI GAANNNNTTC 1 cut(s) 215
PfeI GAWTC 1 cut(s) 756
PflFI GACNNNGTC 1 cut(s) 596
PshBI ATTAAT 1 cut(s) 531
Psp6I CCWGG 2 cut(s) 101, 906
PspGI CCWGG 2 cut(s) 101, 906
PspN4I GGNNCC 4 cut(s) 100, 613, 876, 970
PspPI GGNCC 2 cut(s) 98, 1082
PstI CTGCAG 1 cut(s) 649
PsuI RGATCY 2 cut(s) 816, 874
PsyI GACNNNGTC 1 cut(s) 596
PvuII CAGCTG 1 cut(s) 284
SaqAI TTAA 6 cut(s) 408, 531, 662, 704, 938, 980
Sau3AI GATC 6 cut(s) 159, 816, 874, 883, 1208, 1233
Sau96I GGNCC 2 cut(s) 98, 1082
ScrFI CCNGG 3 cut(s) 103, 117, 908
SduI GDGCHC 1 cut(s) 309
SfaNI GCATC 4 cut(s) 193, 627, 706, 1329
SfcI CTRYAG 1 cut(s) 645
SinI GGWCC 1 cut(s) 1082
SmlI CTYRAG 1 cut(s) 1204
SmoI CTYRAG 1 cut(s) 1204
Sse9I AATT 5 cut(s) 291, 351, 382, 705, 839
SsiI CCGC 7 cut(s) 84, 152, 206, 234, 275, 393, 566
SspMI CTAG 3 cut(s) 182, 683, 1157
StyD4I CCNGG 3 cut(s) 101, 115, 906
StyI CCWWGG 4 cut(s) 377, 441, 1065, 1373
TaaI ACNGT 4 cut(s) 95, 199, 1030, 1201
TaiI ACGT 4 cut(s) 33, 57, 268, 624
TaqI TCGA 4 cut(s) 162, 1020, 1185, 1254
TaqII GACCGA 1 cut(s) 36
TasI AATT 5 cut(s) 291, 351, 382, 705, 839
TauI GCSGC 4 cut(s) 86, 236, 396, 569
TfiI GAWTC 1 cut(s) 756
Tru1I TTAA 6 cut(s) 408, 531, 662, 704, 938, 980
Tru9I TTAA 6 cut(s) 408, 531, 662, 704, 938, 980
TscAI CASTG 2 cut(s) 1042, 1101
TseFI GTSAC 3 cut(s) 693, 1092, 1351
TseI GCWGC 8 cut(s) 74, 281, 428, 644, 647, 734, 919, 1160
Tsp45I GTSAC 3 cut(s) 693, 1092, 1351
TspDTI ATGAA 5 cut(s) 158, 180, 284, 840, 1115
TspGWI ACGGA 1 cut(s) 1205
TspRI CASTG 2 cut(s) 1042, 1101
Tth111I GACNNNGTC 1 cut(s) 596
VpaK11BI GGWCC 1 cut(s) 1082
VspI ATTAAT 1 cut(s) 531
XagI CCTNNNNNAGG 1 cut(s) 1272
XapI RAATTY 2 cut(s) 291, 382
XceI RCATGY 2 cut(s) 400, 1057
XmnI GAANNNNTTC 1 cut(s) 215
XspI CTAG 3 cut(s) 182, 683, 1157
Zsp2I ATGCAT 1 cut(s) 1338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.