Rh1CG381500
MYB Family

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0001154
Physical Location & Seq
Reverse (-)
65133290 .. 65138161
4872 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG381500.1

Sequence Viewer

Length: 1044 bp
ATGCCTCTTTCATCAGTTCCAAAGTCACGCCTCATTCGCGCCTCTGTTTCGCGCCTTTTTCATCACTTACAAAGTCACACTGACACATTCCAAAAGCTCTATATCAAGGGAGACGAATTGGATCATCTTCACTCACAAAGAATTCATCTGATGAGTTCAGCAAATTCTACTCGTAGAAGAAAACAACCAGAACAGCCATCACACAACTGGACCCCTCGTGAAGATGTGGCTCTAGTTGAGGCGCTAACAGAGATATGTGTTGGTGGCTATTGGAAGGTTGACAATGGCTTCAGGTCTGGGTACTTGGGTAAACTTGAACAGATTATAGAGCAAAAGCTACCAAACTGTGGGCTTAAGGCAAACCCCCACATTGAGTCTCGTTTGAAGACATTGAAGAAACAAACACTTGCCATCTACGACATGCTTTCTAACTCAAGTGGGTTCTCATGGAACCATGAAGAAAAAATGGTGGTGTGTGAAAAACAAGTGTTTGGTGATTGGGTTAAAGTTCATAAGGATGCAAAAGGACTGAGAATGAAACCATTCATTCATTATGATAGCTTGATAGAAGCCTTTGGCAGAGATCGTGCAAATGGATTAGGAGCTGAAGGACCAGCTGAAGCAGATGAAGATCTCAATAATGAGAATAACCATGCTGACATTGAAGAAAATGGTTTGGATGAAATCACAGTTCCTTCATCTGTTACCCGCAATTCTGCAAACCCACCTTCAGCTCGAACTAGGAAGCGAGCACGAGATGCTTATTCTCAATGTTTGGGAGACATGGCTAGCACATTACGCTCCTTTGTGGACGTGACCAAGGCACACTTGGAGACAATGCAGGGAGTACTCATGAATGAGCATGTAACATCTGAAAGAAGAGGTAAGCTTGTGGAGGAGCTTATGAAAATTGAAGGCATAAATGACTATGATGTAATAGAAGCTGCTGCTGCAATTATTGGTGATGACTCCAAAATTGACCTCTTGTTCAGTTTGCCAGACAACCTCAAAAGTCAATGGATTCATAAACTTCTTGGCCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4 Myb_DNA-bind_3

Protein Analysis

347

Amino Acids

39.2

Weight (kDa)

6.58

Isoelectric Point (pI)

53.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-bind_3 PF12776 69 - 163 1.1e-06 Myb/SANT-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 347
AccII CGCG 2 cut(s) 39, 52
AciI CCGC 1 cut(s) 709
AclWI GGATC 1 cut(s) 129
AcoI YGGCCR 1 cut(s) 1036
AcsI RAATTY 2 cut(s) 141, 163
AcuI CTGAAG 4 cut(s) 274, 627, 639, 714
AfaI GTAC 2 cut(s) 302, 849
AfiI CCNNNNNNNGG 1 cut(s) 347
AflII CTTAAG 1 cut(s) 353
AgsI TTSAA 5 cut(s) 317, 385, 394, 665, 914
AjiI CACGTC 1 cut(s) 814
AluBI AGCT 9 cut(s) 97, 337, 561, 605, 617, 734, 889, 901, 944
AluI AGCT 9 cut(s) 97, 337, 561, 605, 617, 734, 889, 901, 944
Alw21I GWGCWC 1 cut(s) 754
Alw26I GTCTC 4 cut(s) 105, 381, 774, 827
AlwI GGATC 1 cut(s) 129
AoxI GGCC 1 cut(s) 1036
ApeKI GCWGC 3 cut(s) 944, 947, 950
ApoI RAATTY 2 cut(s) 141, 163
Asp700I GAANNNNTTC 1 cut(s) 542
AspLEI GCGC 3 cut(s) 41, 54, 244
AspS9I GGNCC 2 cut(s) 210, 611
AsuHPI GGTGA 2 cut(s) 506, 974
AsuNHI GCTAGC 1 cut(s) 788
AvaII GGWCC 2 cut(s) 210, 611
BaeI ACNNNNGTAYC 2 cut(s) 292, 325
BauI CACGAG 2 cut(s) 216, 753
BbsI GAAGAC 1 cut(s) 392
Bbv12I GWGCWC 1 cut(s) 754
BbvI GCAGC 3 cut(s) 931, 934, 937
BccI CCATC 2 cut(s) 205, 419
BceAI ACGGC 1 cut(s) 1023
BcoDI GTCTC 4 cut(s) 105, 381, 774, 827
BfaI CTAG 3 cut(s) 233, 741, 789
BfoI RGCGCY 1 cut(s) 245
BfrI CTTAAG 1 cut(s) 353
BglII AGATCT 1 cut(s) 631
BisI GCNGC 3 cut(s) 945, 948, 951
BlsI GCNGC 3 cut(s) 946, 949, 952
BmcAI AGTACT 1 cut(s) 849
Bme18I GGWCC 2 cut(s) 210, 611
BmgBI CACGTC 1 cut(s) 814
BmgT120I GGNCC 2 cut(s) 210, 611
BmiI GGNNCC 2 cut(s) 212, 452
BmsI GCATC 2 cut(s) 508, 748
BmtI GCTAGC 1 cut(s) 792
BpiI GAAGAC 1 cut(s) 392
BpuEI CTTGAG 1 cut(s) 418
BsaBI GATNNNNATC 1 cut(s) 630
BsaJI CCNNGG 1 cut(s) 819
Bsc4I CCNNNNNNNGG 1 cut(s) 347
Bse1I ACTGG 1 cut(s) 212
Bse8I GATNNNNATC 1 cut(s) 630
BseDI CCNNGG 1 cut(s) 819
BseGI GGATG 2 cut(s) 523, 685
BseJI GATNNNNATC 1 cut(s) 630
BseLI CCNNNNNNNGG 1 cut(s) 347
BseMII CTCAG 1 cut(s) 521
BseNI ACTGG 1 cut(s) 212
BseRI GAGGAG 1 cut(s) 911
BseXI GCAGC 3 cut(s) 931, 934, 937
Bsh1236I CGCG 2 cut(s) 39, 52
BshFI GGCC 1 cut(s) 1038
BsiHKAI GWGCWC 1 cut(s) 754
BslI CCNNNNNNNGG 1 cut(s) 347
BsmAI GTCTC 4 cut(s) 105, 381, 774, 827
BsmBI CGTCTC 1 cut(s) 105
BsnI GGCC 1 cut(s) 1038
Bsp1286I GDGCHC 1 cut(s) 754
Bsp143I GATC 3 cut(s) 121, 583, 631
BspACI CCGC 1 cut(s) 709
BspANI GGCC 1 cut(s) 1038
BspCNI CTCAG 1 cut(s) 522
BspFNI CGCG 2 cut(s) 39, 52
BspHI TCATGA 1 cut(s) 852
BspLI GGNNCC 2 cut(s) 212, 452
BspOI GCTAGC 1 cut(s) 792
BspPI GGATC 1 cut(s) 129
BspTI CTTAAG 1 cut(s) 353
BsrI ACTGG 1 cut(s) 212
BssECI CCNNGG 1 cut(s) 819
BssMI GATC 3 cut(s) 121, 583, 631
BssSI CACGAG 2 cut(s) 216, 753
BssT1I CCWWGG 1 cut(s) 819
Bst2BI CACGAG 2 cut(s) 216, 753
Bst4CI ACNGT 2 cut(s) 347, 691
Bst6I CTCTTC 1 cut(s) 874
BstAFI CTTAAG 1 cut(s) 353
BstAPI GCANNNNNTGC 1 cut(s) 758
BstC8I GCNNGC 2 cut(s) 750, 790
BstDEI CTNAG 1 cut(s) 530
BstF5I GGATG 2 cut(s) 523, 685
BstFNI CGCG 2 cut(s) 39, 52
BstH2I RGCGCY 1 cut(s) 245
BstHHI GCGC 3 cut(s) 41, 54, 244
BstKTI GATC 3 cut(s) 124, 586, 634
BstMAI GTCTC 4 cut(s) 105, 381, 774, 827
BstMBI GATC 3 cut(s) 121, 583, 631
BstMWI GCNNNNNNNGC 4 cut(s) 36, 758, 798, 950
BstNSI RCATGY 2 cut(s) 424, 866
BstUI CGCG 2 cut(s) 39, 52
BstV1I GCAGC 3 cut(s) 931, 934, 937
BstV2I GAAGAC 1 cut(s) 392
BstX2I RGATCY 1 cut(s) 631
BstYI RGATCY 1 cut(s) 631
BsuRI GGCC 1 cut(s) 1038
BtrI CACGTC 1 cut(s) 814
BtsCI GGATG 2 cut(s) 523, 685
BtsIMutI CAGTG 1 cut(s) 78
Cac8I GCNNGC 2 cut(s) 750, 790
CciI TCATGA 1 cut(s) 852
CfoI GCGC 3 cut(s) 41, 54, 244
Cfr13I GGNCC 2 cut(s) 210, 611
Csp6I GTAC 2 cut(s) 301, 848
CviAII CATG 7 cut(s) 421, 447, 455, 653, 784, 853, 863
CviQI GTAC 2 cut(s) 301, 848
DdeI CTNAG 1 cut(s) 530
DpnI GATC 3 cut(s) 123, 585, 633
DpnII GATC 3 cut(s) 121, 583, 631
EaeI YGGCCR 1 cut(s) 1036
Eam1104I CTCTTC 1 cut(s) 874
EarI CTCTTC 1 cut(s) 874
Eco130I CCWWGG 1 cut(s) 819
Eco47I GGWCC 2 cut(s) 210, 611
Eco57I CTGAAG 4 cut(s) 274, 627, 639, 714
EcoRI GAATTC 1 cut(s) 141
EcoT14I CCWWGG 1 cut(s) 819
ErhI CCWWGG 1 cut(s) 819
Esp3I CGTCTC 1 cut(s) 105
FaeI CATG 7 cut(s) 424, 450, 458, 656, 787, 856, 866
FalI AAGNNNNNCTT 2 cut(s) 812, 844
FatI CATG 7 cut(s) 420, 446, 454, 652, 783, 852, 862
FauI CCCGC 1 cut(s) 716
Fnu4HI GCNGC 3 cut(s) 945, 948, 951
FokI GGATG 2 cut(s) 530, 692
Fsp4HI GCNGC 3 cut(s) 945, 948, 951
FspBI CTAG 3 cut(s) 233, 741, 789
GlaI GCGC 3 cut(s) 40, 53, 243
GluI GCNGC 3 cut(s) 945, 948, 951
HaeII RGCGCY 1 cut(s) 245
HaeIII GGCC 1 cut(s) 1038
HhaI GCGC 3 cut(s) 41, 54, 244
Hin1II CATG 7 cut(s) 424, 450, 458, 656, 787, 856, 866
Hin6I GCGC 3 cut(s) 39, 52, 242
HinP1I GCGC 3 cut(s) 39, 52, 242
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HindIII AAGCTT 1 cut(s) 887
HinfI GANTC 3 cut(s) 374, 968, 1021
HphI GGTGA 2 cut(s) 506, 974
Hpy166II GTNNAC 3 cut(s) 280, 311, 811
Hpy188I TCNGA 2 cut(s) 150, 874
Hpy188III TCNNGA 2 cut(s) 218, 853
Hpy8I GTNNAC 3 cut(s) 280, 311, 811
HpyAV CCTTC 5 cut(s) 268, 602, 705, 738, 908
HpyCH4III ACNGT 2 cut(s) 347, 691
HpyCH4IV ACGT 1 cut(s) 813
HpyCH4V TGCA 5 cut(s) 521, 590, 719, 841, 953
HpyF10VI GCNNNNNNNGC 4 cut(s) 36, 758, 798, 950
HpyF3I CTNAG 1 cut(s) 530
HpySE526I ACGT 1 cut(s) 813
Hsp92II CATG 7 cut(s) 424, 450, 458, 656, 787, 856, 866
HspAI GCGC 3 cut(s) 39, 52, 242
Kzo9I GATC 3 cut(s) 121, 583, 631
LmnI GCTCC 3 cut(s) 602, 806, 898
LpnPI CCDG 7 cut(s) 193, 201, 277, 282, 627, 827, 1011
Lsp1109I GCAGC 3 cut(s) 931, 934, 937
LweI GCATC 2 cut(s) 508, 748
MaeI CTAG 3 cut(s) 233, 741, 789
MaeII ACGT 1 cut(s) 813
MaeIII GTNAC 5 cut(s) 24, 74, 703, 814, 865
MalI GATC 3 cut(s) 123, 585, 633
MboI GATC 3 cut(s) 121, 583, 631
MboII GAAGA 9 cut(s) 119, 189, 233, 397, 406, 470, 641, 677, 891
MflI RGATCY 1 cut(s) 631
MhlI GDGCHC 1 cut(s) 754
MluCI AATT 7 cut(s) 116, 141, 163, 712, 909, 954, 975
MlyI GAGTC 2 cut(s) 383, 962
MnlI CCTC 9 cut(s) 15, 41, 52, 225, 232, 875, 889, 992, 1016
MroXI GAANNNNTTC 1 cut(s) 542
MseI TTAA 2 cut(s) 354, 504
MslI CAYNNNNRTG 1 cut(s) 516
MspA1I CMGCKG 1 cut(s) 617
MspCI CTTAAG 1 cut(s) 353
MvnI CGCG 2 cut(s) 39, 52
MwoI GCNNNNNNNGC 4 cut(s) 36, 758, 798, 950
NdeII GATC 3 cut(s) 121, 583, 631
NheI GCTAGC 1 cut(s) 788
NlaIII CATG 7 cut(s) 424, 450, 458, 656, 787, 856, 866
NlaIV GGNNCC 2 cut(s) 212, 452
NmuCI GTSAC 3 cut(s) 24, 74, 814
NspI RCATGY 2 cut(s) 424, 866
PagI TCATGA 1 cut(s) 852
PdmI GAANNNNTTC 1 cut(s) 542
PfeI GAWTC 1 cut(s) 1021
PflMI CCANNNNNTGG 1 cut(s) 347
PkrI GCNGC 3 cut(s) 946, 949, 952
PleI GAGTC 2 cut(s) 382, 962
PpsI GAGTC 2 cut(s) 382, 962
PspN4I GGNNCC 2 cut(s) 212, 452
PspPI GGNCC 2 cut(s) 210, 611
PsuI RGATCY 1 cut(s) 631
PvuII CAGCTG 1 cut(s) 617
RsaI GTAC 2 cut(s) 302, 849
RsaNI GTAC 2 cut(s) 301, 848
RseI CAYNNNNRTG 1 cut(s) 516
SaqAI TTAA 2 cut(s) 354, 504
SatI GCNGC 3 cut(s) 945, 948, 951
Sau3AI GATC 3 cut(s) 121, 583, 631
Sau96I GGNCC 2 cut(s) 210, 611
ScaI AGTACT 1 cut(s) 849
SchI GAGTC 2 cut(s) 383, 962
SduI GDGCHC 1 cut(s) 754
SfaNI GCATC 2 cut(s) 508, 748
SinI GGWCC 2 cut(s) 210, 611
SmiMI CAYNNNNRTG 1 cut(s) 516
SmlI CTYRAG 2 cut(s) 353, 433
SmoI CTYRAG 2 cut(s) 353, 433
Sse9I AATT 7 cut(s) 116, 141, 163, 712, 909, 954, 975
SsiI CCGC 1 cut(s) 709
SspMI CTAG 3 cut(s) 233, 741, 789
StyI CCWWGG 1 cut(s) 819
TaaI ACNGT 2 cut(s) 347, 691
TaiI ACGT 1 cut(s) 816
TaqI TCGA 1 cut(s) 736
TasI AATT 7 cut(s) 116, 141, 163, 712, 909, 954, 975
TatI WGTACW 1 cut(s) 847
TfiI GAWTC 1 cut(s) 1021
Tru1I TTAA 2 cut(s) 354, 504
Tru9I TTAA 2 cut(s) 354, 504
TscAI CASTG 1 cut(s) 85
TseFI GTSAC 3 cut(s) 24, 74, 814
TseI GCWGC 3 cut(s) 944, 947, 950
Tsp45I GTSAC 3 cut(s) 24, 74, 814
TspRI CASTG 1 cut(s) 85
Van91I CCANNNNNTGG 1 cut(s) 347
Vha464I CTTAAG 1 cut(s) 353
VpaK11BI GGWCC 2 cut(s) 210, 611
XapI RAATTY 2 cut(s) 141, 163
XceI RCATGY 2 cut(s) 424, 866
XcmI CCANNNNNNNNNTGG 2 cut(s) 204, 826
XmnI GAANNNNTTC 1 cut(s) 542
XspI CTAG 3 cut(s) 233, 741, 789
ZrmI AGTACT 1 cut(s) 849
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.