nuclease HARBI1
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 289 |
| Acc36I | ACCTGC | 2 cut(s) | 202, 289 |
| AciI | CCGC | 1 cut(s) | 303 |
| AcsI | RAATTY | 1 cut(s) | 230 |
| AcuI | CTGAAG | 2 cut(s) | 207, 219 |
| AfaI | GTAC | 1 cut(s) | 429 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 28 |
| AgsI | TTSAA | 7 cut(s) | 59, 217, 245, 315, 436, 494, 502 |
| AluBI | AGCT | 9 cut(s) | 152, 170, 185, 203, 469, 481, 562, 573, 610 |
| AluI | AGCT | 9 cut(s) | 152, 170, 185, 203, 469, 481, 562, 573, 610 |
| Alw26I | GTCTC | 3 cut(s) | 333, 357, 410 |
| AoxI | GGCC | 1 cut(s) | 19 |
| ApeKI | GCWGC | 1 cut(s) | 527 |
| ApoI | RAATTY | 1 cut(s) | 230 |
| AspS9I | GGNCC | 2 cut(s) | 31, 191 |
| AsuHPI | GGTGA | 1 cut(s) | 554 |
| AvaII | GGWCC | 2 cut(s) | 31, 191 |
| BbvI | GCAGC | 1 cut(s) | 514 |
| BcoDI | GTCTC | 3 cut(s) | 333, 357, 410 |
| BfaI | CTAG | 2 cut(s) | 324, 622 |
| BfuAI | ACCTGC | 2 cut(s) | 202, 289 |
| BisI | GCNGC | 1 cut(s) | 528 |
| BlsI | GCNGC | 1 cut(s) | 529 |
| BmcAI | AGTACT | 1 cut(s) | 429 |
| Bme18I | GGWCC | 2 cut(s) | 31, 191 |
| BmgT120I | GGNCC | 2 cut(s) | 31, 191 |
| BmiI | GGNNCC | 1 cut(s) | 32 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 354, 384 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 28 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 28 |
| BseMII | CTCAG | 1 cut(s) | 510 |
| BseXI | GCAGC | 1 cut(s) | 514 |
| BshFI | GGCC | 1 cut(s) | 21 |
| BslFI | GGGAC | 1 cut(s) | 44 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 28 |
| BsmAI | GTCTC | 3 cut(s) | 333, 357, 410 |
| BsmBI | CGTCTC | 1 cut(s) | 333 |
| BsmFI | GGGAC | 1 cut(s) | 44 |
| BsnI | GGCC | 1 cut(s) | 21 |
| Bsp143I | GATC | 1 cut(s) | 238 |
| BspACI | CCGC | 1 cut(s) | 303 |
| BspANI | GGCC | 1 cut(s) | 21 |
| BspCNI | CTCAG | 1 cut(s) | 511 |
| BspLI | GGNNCC | 1 cut(s) | 32 |
| BspMI | ACCTGC | 2 cut(s) | 202, 289 |
| BssMI | GATC | 1 cut(s) | 238 |
| BstC8I | GCNNGC | 1 cut(s) | 333 |
| BstDEI | CTNAG | 2 cut(s) | 110, 519 |
| BstKTI | GATC | 1 cut(s) | 241 |
| BstMAI | GTCTC | 3 cut(s) | 333, 357, 410 |
| BstMBI | GATC | 1 cut(s) | 238 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 341, 381 |
| BstV1I | GCAGC | 1 cut(s) | 514 |
| BsuRI | GGCC | 1 cut(s) | 21 |
| BveI | ACCTGC | 2 cut(s) | 202, 289 |
| Cac8I | GCNNGC | 1 cut(s) | 333 |
| Cfr13I | GGNCC | 2 cut(s) | 31, 191 |
| CseI | GACGC | 1 cut(s) | 350 |
| Csp6I | GTAC | 1 cut(s) | 428 |
| CviAII | CATG | 4 cut(s) | 27, 35, 367, 443 |
| CviQI | GTAC | 1 cut(s) | 428 |
| DdeI | CTNAG | 2 cut(s) | 110, 519 |
| DpnI | GATC | 1 cut(s) | 240 |
| DpnII | GATC | 1 cut(s) | 238 |
| DraI | TTTAAA | 1 cut(s) | 588 |
| Eco47I | GGWCC | 2 cut(s) | 31, 191 |
| Eco57I | CTGAAG | 2 cut(s) | 207, 219 |
| EcoO109I | RGGNCCY | 1 cut(s) | 191 |
| Esp3I | CGTCTC | 1 cut(s) | 333 |
| FaeI | CATG | 4 cut(s) | 30, 38, 370, 446 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 328, 360, 395, 427 |
| FaqI | GGGAC | 1 cut(s) | 44 |
| FatI | CATG | 4 cut(s) | 26, 34, 366, 442 |
| Fnu4HI | GCNGC | 1 cut(s) | 528 |
| Fsp4HI | GCNGC | 1 cut(s) | 528 |
| FspBI | CTAG | 2 cut(s) | 324, 622 |
| GluI | GCNGC | 1 cut(s) | 528 |
| HaeIII | GGCC | 1 cut(s) | 21 |
| HgaI | GACGC | 1 cut(s) | 350 |
| Hin1II | CATG | 4 cut(s) | 30, 38, 370, 446 |
| HindIII | AAGCTT | 3 cut(s) | 150, 467, 608 |
| HinfI | GANTC | 1 cut(s) | 548 |
| HphI | GGTGA | 1 cut(s) | 554 |
| Hpy166II | GTNNAC | 1 cut(s) | 91 |
| Hpy188I | TCNGA | 1 cut(s) | 520 |
| Hpy188III | TCNNGA | 1 cut(s) | 242 |
| Hpy8I | GTNNAC | 1 cut(s) | 91 |
| HpyAV | CCTTC | 4 cut(s) | 182, 239, 245, 488 |
| HpyCH4V | TGCA | 1 cut(s) | 446 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 341, 381 |
| HpyF3I | CTNAG | 2 cut(s) | 110, 519 |
| Hsp92II | CATG | 4 cut(s) | 30, 38, 370, 446 |
| Kzo9I | GATC | 1 cut(s) | 238 |
| LmnI | GCTCC | 2 cut(s) | 182, 389 |
| LpnPI | CCDG | 4 cut(s) | 207, 273, 294, 591 |
| Lsp1109I | GCAGC | 1 cut(s) | 514 |
| MaeI | CTAG | 2 cut(s) | 324, 622 |
| MalI | GATC | 1 cut(s) | 240 |
| MboI | GATC | 1 cut(s) | 238 |
| MboII | GAAGA | 2 cut(s) | 221, 291 |
| MluCI | AATT | 5 cut(s) | 230, 310, 489, 534, 555 |
| MlyI | GAGTC | 1 cut(s) | 542 |
| MnlI | CCTC | 3 cut(s) | 32, 455, 514 |
| MseI | TTAA | 4 cut(s) | 84, 117, 587, 615 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 341, 381 |
| NdeII | GATC | 1 cut(s) | 238 |
| NlaIII | CATG | 4 cut(s) | 30, 38, 370, 446 |
| NlaIV | GGNNCC | 1 cut(s) | 32 |
| PaqCI | CACCTGC | 1 cut(s) | 289 |
| PkrI | GCNGC | 1 cut(s) | 529 |
| PleI | GAGTC | 1 cut(s) | 542 |
| PpsI | GAGTC | 1 cut(s) | 542 |
| PpuMI | RGGWCCY | 1 cut(s) | 191 |
| Psp5II | RGGWCCY | 1 cut(s) | 191 |
| PspN4I | GGNNCC | 1 cut(s) | 32 |
| PspPI | GGNCC | 2 cut(s) | 31, 191 |
| PspPPI | RGGWCCY | 1 cut(s) | 191 |
| RsaI | GTAC | 1 cut(s) | 429 |
| RsaNI | GTAC | 1 cut(s) | 428 |
| SaqAI | TTAA | 4 cut(s) | 84, 117, 587, 615 |
| SatI | GCNGC | 1 cut(s) | 528 |
| Sau3AI | GATC | 1 cut(s) | 238 |
| Sau96I | GGNCC | 2 cut(s) | 31, 191 |
| ScaI | AGTACT | 1 cut(s) | 429 |
| SchI | GAGTC | 1 cut(s) | 542 |
| SinI | GGWCC | 2 cut(s) | 31, 191 |
| SmlI | CTYRAG | 1 cut(s) | 13 |
| SmoI | CTYRAG | 1 cut(s) | 13 |
| Sse9I | AATT | 5 cut(s) | 230, 310, 489, 534, 555 |
| SsiI | CCGC | 1 cut(s) | 303 |
| SspI | AATATT | 1 cut(s) | 268 |
| SspMI | CTAG | 2 cut(s) | 324, 622 |
| TaqI | TCGA | 1 cut(s) | 319 |
| TasI | AATT | 5 cut(s) | 230, 310, 489, 534, 555 |
| TatI | WGTACW | 1 cut(s) | 427 |
| Tru1I | TTAA | 4 cut(s) | 84, 117, 587, 615 |
| Tru9I | TTAA | 4 cut(s) | 84, 117, 587, 615 |
| TseI | GCWGC | 1 cut(s) | 527 |
| TspDTI | ATGAA | 7 cut(s) | 51, 115, 119, 222, 243, 437, 500 |
| VpaK11BI | GGWCC | 2 cut(s) | 31, 191 |
| XapI | RAATTY | 1 cut(s) | 230 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 409 |
| XspI | CTAG | 2 cut(s) | 324, 622 |
| ZrmI | AGTACT | 1 cut(s) | 429 |