Rw5G026590
MYB Family

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001154
Physical Location & Seq
Forward (+)
38506283 .. 38507216
934 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G026590.1

Sequence Viewer

Length: 579 bp
ATGGTGGTGTGTGAAAAACAAGTGTTTGATGATTGGGTTAAAGTTCATAAGGATGCAAAAGGACTGAGAATGAAACCATTCATTCATTATGATAGCTTGATAGAAGCCTTTGGCAGAGACCGTGCAAATGGATTAGGAGCTGAAGGACCAGCTGAAGCAAATGAAGATCTCAATAATGAGAATAACTATGCTGACATTGAAGAAAATGGTTTGGATGAAATCACAGTTCCTTCATCTGTTACCCGTAATTCTGCAAACCCACCTTCAGCTCGAACTAGGAAGCGAGCACGAGATGCTTATTCTCAATGTTTGGGAGACATGGCTAGCACATTACGCTCCTTTGTGGACGTGACCAAGGCACACTTTGAGACAATGCAGGGAGTACTCATGAATGAGCATGTAACATCTGAAAGAAGAGGAAAGCTTGTGGAGGAGCTTATGAAAATAGAAGGCATAAATGACTATGATGTAATAGAAGCTGCTGCTGCAATTATTGGTGATGACTCCAAAATTGACCTCTTGTTCAGTTTGCCAGACAACCTCAAAAGTCAATGGATTCATAAACTTCTTGGCCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4 Myb_DNA-bind_3

Protein Analysis

192

Amino Acids

21.47

Weight (kDa)

5.06

Isoelectric Point (pI)

46.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 571
AcuI CTGAAG 3 cut(s) 162, 174, 249
AfaI GTAC 1 cut(s) 384
AgsI TTSAA 1 cut(s) 200
AjiI CACGTC 1 cut(s) 349
AluBI AGCT 7 cut(s) 96, 140, 152, 269, 424, 436, 479
AluI AGCT 7 cut(s) 96, 140, 152, 269, 424, 436, 479
Alw21I GWGCWC 1 cut(s) 289
Alw26I GTCTC 3 cut(s) 111, 309, 362
AoxI GGCC 1 cut(s) 571
ApeKI GCWGC 3 cut(s) 479, 482, 485
Asp700I GAANNNNTTC 1 cut(s) 77
AspS9I GGNCC 1 cut(s) 146
AsuHPI GGTGA 1 cut(s) 509
AsuNHI GCTAGC 1 cut(s) 323
AvaII GGWCC 1 cut(s) 146
BalI TGGCCA 1 cut(s) 573
BauI CACGAG 1 cut(s) 288
Bbv12I GWGCWC 1 cut(s) 289
BbvI GCAGC 3 cut(s) 466, 469, 472
BcoDI GTCTC 3 cut(s) 111, 309, 362
BfaI CTAG 2 cut(s) 276, 324
BglII AGATCT 1 cut(s) 166
BisI GCNGC 3 cut(s) 480, 483, 486
BlsI GCNGC 3 cut(s) 481, 484, 487
BmcAI AGTACT 1 cut(s) 384
Bme18I GGWCC 1 cut(s) 146
BmgBI CACGTC 1 cut(s) 349
BmgT120I GGNCC 1 cut(s) 146
BmsI GCATC 2 cut(s) 43, 283
BmtI GCTAGC 1 cut(s) 327
BsaI GGTCTC 1 cut(s) 111
BsaJI CCNNGG 1 cut(s) 354
BseDI CCNNGG 1 cut(s) 354
BseGI GGATG 2 cut(s) 58, 220
BseMII CTCAG 1 cut(s) 56
BseRI GAGGAG 1 cut(s) 446
BseXI GCAGC 3 cut(s) 466, 469, 472
BshFI GGCC 1 cut(s) 573
BsiHKAI GWGCWC 1 cut(s) 289
BsmAI GTCTC 3 cut(s) 111, 309, 362
BsnI GGCC 1 cut(s) 573
Bso31I GGTCTC 1 cut(s) 111
Bsp1286I GDGCHC 1 cut(s) 289
Bsp143I GATC 1 cut(s) 166
BspANI GGCC 1 cut(s) 573
BspCNI CTCAG 1 cut(s) 57
BspHI TCATGA 1 cut(s) 387
BspOI GCTAGC 1 cut(s) 327
BspTNI GGTCTC 1 cut(s) 111
BssECI CCNNGG 1 cut(s) 354
BssMI GATC 1 cut(s) 166
BssSI CACGAG 1 cut(s) 288
BssT1I CCWWGG 1 cut(s) 354
Bst2BI CACGAG 1 cut(s) 288
Bst4CI ACNGT 2 cut(s) 122, 226
Bst6I CTCTTC 1 cut(s) 409
BstAPI GCANNNNNTGC 1 cut(s) 293
BstC8I GCNNGC 2 cut(s) 285, 325
BstDEI CTNAG 1 cut(s) 65
BstF5I GGATG 2 cut(s) 58, 220
BstKTI GATC 1 cut(s) 169
BstMAI GTCTC 3 cut(s) 111, 309, 362
BstMBI GATC 1 cut(s) 166
BstMWI GCNNNNNNNGC 3 cut(s) 293, 333, 485
BstNSI RCATGY 1 cut(s) 401
BstV1I GCAGC 3 cut(s) 466, 469, 472
BstX2I RGATCY 1 cut(s) 166
BstYI RGATCY 1 cut(s) 166
BsuRI GGCC 1 cut(s) 573
BtrI CACGTC 1 cut(s) 349
BtsCI GGATG 2 cut(s) 58, 220
Cac8I GCNNGC 2 cut(s) 285, 325
CciI TCATGA 1 cut(s) 387
Cfr13I GGNCC 1 cut(s) 146
Csp6I GTAC 1 cut(s) 383
CviAII CATG 3 cut(s) 319, 388, 398
CviQI GTAC 1 cut(s) 383
DdeI CTNAG 1 cut(s) 65
DpnI GATC 1 cut(s) 168
DpnII GATC 1 cut(s) 166
EaeI YGGCCR 1 cut(s) 571
Eam1104I CTCTTC 1 cut(s) 409
EarI CTCTTC 1 cut(s) 409
Eco130I CCWWGG 1 cut(s) 354
Eco31I GGTCTC 1 cut(s) 111
Eco47I GGWCC 1 cut(s) 146
Eco57I CTGAAG 3 cut(s) 162, 174, 249
EcoT14I CCWWGG 1 cut(s) 354
ErhI CCWWGG 1 cut(s) 354
FaeI CATG 3 cut(s) 322, 391, 401
FalI AAGNNNNNCTT 2 cut(s) 347, 379
FatI CATG 3 cut(s) 318, 387, 397
Fnu4HI GCNGC 3 cut(s) 480, 483, 486
FokI GGATG 2 cut(s) 65, 227
Fsp4HI GCNGC 3 cut(s) 480, 483, 486
FspBI CTAG 2 cut(s) 276, 324
GluI GCNGC 3 cut(s) 480, 483, 486
HaeIII GGCC 1 cut(s) 573
Hin1II CATG 3 cut(s) 322, 391, 401
HindIII AAGCTT 1 cut(s) 422
HinfI GANTC 2 cut(s) 503, 556
HphI GGTGA 1 cut(s) 509
Hpy166II GTNNAC 1 cut(s) 346
Hpy188I TCNGA 1 cut(s) 409
Hpy188III TCNNGA 1 cut(s) 388
Hpy8I GTNNAC 1 cut(s) 346
HpyAV CCTTC 4 cut(s) 137, 240, 273, 443
HpyCH4III ACNGT 2 cut(s) 122, 226
HpyCH4IV ACGT 1 cut(s) 348
HpyCH4V TGCA 5 cut(s) 56, 125, 254, 376, 488
HpyF10VI GCNNNNNNNGC 3 cut(s) 293, 333, 485
HpyF3I CTNAG 1 cut(s) 65
HpySE526I ACGT 1 cut(s) 348
Hsp92II CATG 3 cut(s) 322, 391, 401
Kzo9I GATC 1 cut(s) 166
LmnI GCTCC 3 cut(s) 137, 341, 433
LpnPI CCDG 3 cut(s) 162, 362, 546
Lsp1109I GCAGC 3 cut(s) 466, 469, 472
LweI GCATC 2 cut(s) 43, 283
MaeI CTAG 2 cut(s) 276, 324
MaeII ACGT 1 cut(s) 348
MaeIII GTNAC 3 cut(s) 238, 349, 400
MalI GATC 1 cut(s) 168
MboI GATC 1 cut(s) 166
MboII GAAGA 3 cut(s) 176, 212, 426
MflI RGATCY 1 cut(s) 166
MhlI GDGCHC 1 cut(s) 289
MlsI TGGCCA 1 cut(s) 573
MluCI AATT 3 cut(s) 247, 489, 510
MluNI TGGCCA 1 cut(s) 573
MlyI GAGTC 1 cut(s) 497
MnlI CCTC 4 cut(s) 410, 424, 527, 551
Mox20I TGGCCA 1 cut(s) 573
MroXI GAANNNNTTC 1 cut(s) 77
MscI TGGCCA 1 cut(s) 573
MseI TTAA 1 cut(s) 39
MslI CAYNNNNRTG 1 cut(s) 51
Msp20I TGGCCA 1 cut(s) 573
MspA1I CMGCKG 1 cut(s) 152
MwoI GCNNNNNNNGC 3 cut(s) 293, 333, 485
NdeII GATC 1 cut(s) 166
NheI GCTAGC 1 cut(s) 323
NlaIII CATG 3 cut(s) 322, 391, 401
NmuCI GTSAC 1 cut(s) 349
NspI RCATGY 1 cut(s) 401
PagI TCATGA 1 cut(s) 387
PdmI GAANNNNTTC 1 cut(s) 77
PfeI GAWTC 1 cut(s) 556
PkrI GCNGC 3 cut(s) 481, 484, 487
PleI GAGTC 1 cut(s) 497
PpsI GAGTC 1 cut(s) 497
PspPI GGNCC 1 cut(s) 146
PsuI RGATCY 1 cut(s) 166
PvuII CAGCTG 1 cut(s) 152
RsaI GTAC 1 cut(s) 384
RsaNI GTAC 1 cut(s) 383
RseI CAYNNNNRTG 1 cut(s) 51
SaqAI TTAA 1 cut(s) 39
SatI GCNGC 3 cut(s) 480, 483, 486
Sau3AI GATC 1 cut(s) 166
Sau96I GGNCC 1 cut(s) 146
ScaI AGTACT 1 cut(s) 384
SchI GAGTC 1 cut(s) 497
SduI GDGCHC 1 cut(s) 289
SfaNI GCATC 2 cut(s) 43, 283
SinI GGWCC 1 cut(s) 146
SmiMI CAYNNNNRTG 1 cut(s) 51
Sse9I AATT 3 cut(s) 247, 489, 510
SspMI CTAG 2 cut(s) 276, 324
StyI CCWWGG 1 cut(s) 354
TaaI ACNGT 2 cut(s) 122, 226
TaiI ACGT 1 cut(s) 351
TaqI TCGA 1 cut(s) 271
TasI AATT 3 cut(s) 247, 489, 510
TatI WGTACW 1 cut(s) 382
TfiI GAWTC 1 cut(s) 556
Tru1I TTAA 1 cut(s) 39
Tru9I TTAA 1 cut(s) 39
TseFI GTSAC 1 cut(s) 349
TseI GCWGC 3 cut(s) 479, 482, 485
Tsp45I GTSAC 1 cut(s) 349
VpaK11BI GGWCC 1 cut(s) 146
XceI RCATGY 1 cut(s) 401
XmnI GAANNNNTTC 1 cut(s) 77
XspI CTAG 2 cut(s) 276, 324
ZrmI AGTACT 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.