Rh5AG318400
MYB Family

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001154
Physical Location & Seq
Forward (+)
46842153 .. 46843350
1198 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG318400.1

Sequence Viewer

Length: 804 bp
ATGGATTTGCAAGCAAACTCAAGATCCTCAACTGCTAGAAGTGGAAGAAATAAGGAGAAGCAACCATCACACACCTGGACCCCTATTGAATATGTAGCTCTAGTTGAGGCTTTGACTGAACTTTGTGTTTCTGGGGTATGGAAGGTTGACAATGGATTCAAGGCAGGATATTTGGGACAACTAGAAAAAATTATAGAGCAAAAGCTACCAAATTGTGGATTAAAGGCGAATCCCCACATTGAGTCTCGTGTGAAGACACTAAAGAAACAAACACTTACAATCTCTGACATGATTGCTAACTCCAGTGGCTTCTCATGGGACCATGAAAATAAAATGGTTGTTTGTGAAAAACAAGTTTATGACGATTGGGTTAAGGTTCACAAGGAAGCCAAAGGACTAAGACTTAAACCATTCATTCATTATGATAGACCTGCAGAAGCTGAAAATATTGTTCCTACACCTGCTACTCGTACAACTTCCTTCGCTTCTAATTCAACTCGGACTAGAAAGCGAGCAAGGGACGCTTATGCTCAATGCTTGGGAGACATGGCTAGCACACTTCGCTCTTTTGTGGATGTGACCAAGACACACTTGGAGATTATGAAAGAAGTACTTTTGAATGACCATGCAGCATCTACAAAAAGAGGTAAACTTGTCGAAGAGCTTATGAAAATTGAAGGTTTGAATGATTATGATGTGCTTGAGGCTGCTGCTATAATTATTGGCGATGACTCCAAAATTGAGCTCTTGTTTAGCTTACCTGACAATTTGAAGAGCCAATGGATTTACAAGCTTCTGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4 Myb_DNA-bind_3

Protein Analysis

267

Amino Acids

30.12

Weight (kDa)

8.53

Isoelectric Point (pI)

48.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-bind_3 PF12776 26 - 121 6.8e-07 Myb/SANT-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 469
Acc36I ACCTGC 2 cut(s) 439, 469
AccB7I CCANNNNNTGG 1 cut(s) 215
AclWI GGATC 1 cut(s) 18
AfaI GTAC 2 cut(s) 472, 612
AfiI CCNNNNNNNGG 1 cut(s) 215
AgsI TTSAA 7 cut(s) 89, 160, 495, 619, 677, 685, 772
AjnI CCWGG 1 cut(s) 74
AluBI AGCT 7 cut(s) 98, 205, 440, 664, 745, 756, 793
AluI AGCT 7 cut(s) 98, 205, 440, 664, 745, 756, 793
Alw21I GWGCWC 1 cut(s) 747
Alw26I GTCTC 2 cut(s) 249, 537
AlwI GGATC 1 cut(s) 18
AlwNI CAGNNNCTG 1 cut(s) 440
ApeKI GCWGC 3 cut(s) 629, 707, 710
AspS9I GGNCC 2 cut(s) 78, 319
AsuNHI GCTAGC 1 cut(s) 551
AvaII GGWCC 2 cut(s) 78, 319
BanII GRGCYC 1 cut(s) 747
BauI CACGAG 1 cut(s) 246
BbsI GAAGAC 1 cut(s) 260
Bbv12I GWGCWC 1 cut(s) 747
BbvI GCAGC 3 cut(s) 641, 694, 697
BccI CCATC 1 cut(s) 73
BciT130I CCWGG 1 cut(s) 76
BcoDI GTCTC 2 cut(s) 249, 537
BfaI CTAG 6 cut(s) 36, 101, 182, 504, 552, 802
BfmI CTRYAG 1 cut(s) 432
BfuAI ACCTGC 2 cut(s) 439, 469
BisI GCNGC 3 cut(s) 630, 708, 711
BlsI GCNGC 3 cut(s) 631, 709, 712
BmcAI AGTACT 1 cut(s) 612
Bme1390I CCNGG 1 cut(s) 76
Bme18I GGWCC 2 cut(s) 78, 319
BmgT120I GGNCC 2 cut(s) 78, 319
BmiI GGNNCC 2 cut(s) 80, 320
BmrFI CCNGG 1 cut(s) 76
BmsI GCATC 1 cut(s) 641
BmtI GCTAGC 1 cut(s) 555
BpiI GAAGAC 1 cut(s) 260
BpmI CTGGAG 1 cut(s) 286
BpuEI CTTGAG 2 cut(s) 4, 722
Bsc4I CCNNNNNNNGG 1 cut(s) 215
Bse1I ACTGG 1 cut(s) 303
BseBI CCWGG 1 cut(s) 76
BseGI GGATG 1 cut(s) 580
BseLI CCNNNNNNNGG 1 cut(s) 215
BseNI ACTGG 1 cut(s) 303
BseXI GCAGC 3 cut(s) 641, 694, 697
BsiHKAI GWGCWC 1 cut(s) 747
BslFI GGGAC 3 cut(s) 189, 332, 533
BslI CCNNNNNNNGG 1 cut(s) 215
BsmAI GTCTC 2 cut(s) 249, 537
BsmFI GGGAC 3 cut(s) 189, 332, 533
Bsp1286I GDGCHC 1 cut(s) 747
Bsp143I GATC 1 cut(s) 23
BspLI GGNNCC 2 cut(s) 80, 320
BspMAI CTGCAG 1 cut(s) 436
BspMI ACCTGC 2 cut(s) 439, 469
BspOI GCTAGC 1 cut(s) 555
BspPI GGATC 1 cut(s) 18
BspQI GCTCTTC 2 cut(s) 654, 767
BsrI ACTGG 1 cut(s) 303
BssMI GATC 1 cut(s) 23
BssSI CACGAG 1 cut(s) 246
Bst2BI CACGAG 1 cut(s) 246
Bst2UI CCWGG 1 cut(s) 76
Bst6I CTCTTC 2 cut(s) 654, 767
BstC8I GCNNGC 3 cut(s) 12, 513, 553
BstDEI CTNAG 1 cut(s) 398
BstF5I GGATG 1 cut(s) 580
BstKTI GATC 1 cut(s) 26
BstMAI GTCTC 2 cut(s) 249, 537
BstMBI GATC 1 cut(s) 23
BstMWI GCNNNNNNNGC 2 cut(s) 521, 561
BstNI CCWGG 1 cut(s) 76
BstSCI CCNGG 1 cut(s) 74
BstSFI CTRYAG 1 cut(s) 432
BstV1I GCAGC 3 cut(s) 641, 694, 697
BstV2I GAAGAC 1 cut(s) 260
BstX2I RGATCY 1 cut(s) 23
BstYI RGATCY 1 cut(s) 23
BtgZI GCGATG 1 cut(s) 741
BtsCI GGATG 1 cut(s) 580
BtsIMutI CAGTG 1 cut(s) 310
BveI ACCTGC 2 cut(s) 439, 469
Cac8I GCNNGC 3 cut(s) 12, 513, 553
CaiI CAGNNNCTG 1 cut(s) 440
Cfr13I GGNCC 2 cut(s) 78, 319
CseI GACGC 1 cut(s) 530
Csp6I GTAC 2 cut(s) 471, 611
CviAII CATG 5 cut(s) 289, 315, 323, 547, 626
CviQI GTAC 2 cut(s) 471, 611
DdeI CTNAG 1 cut(s) 398
DpnI GATC 1 cut(s) 25
DpnII GATC 1 cut(s) 23
Eam1104I CTCTTC 2 cut(s) 654, 767
EarI CTCTTC 2 cut(s) 654, 767
Ecl136II GAGCTC 1 cut(s) 745
Eco24I GRGCYC 1 cut(s) 747
Eco47I GGWCC 2 cut(s) 78, 319
Eco53kI GAGCTC 1 cut(s) 745
EcoICRI GAGCTC 1 cut(s) 745
EcoRII CCWGG 1 cut(s) 74
EcoT38I GRGCYC 1 cut(s) 747
FaeI CATG 5 cut(s) 292, 318, 326, 550, 629
FalI AAGNNNNNCTT 6 cut(s) 508, 540, 575, 607, 597, 629
FaqI GGGAC 3 cut(s) 189, 332, 533
FatI CATG 5 cut(s) 288, 314, 322, 546, 625
Fnu4HI GCNGC 3 cut(s) 630, 708, 711
FokI GGATG 1 cut(s) 587
FriOI GRGCYC 1 cut(s) 747
Fsp4HI GCNGC 3 cut(s) 630, 708, 711
FspBI CTAG 6 cut(s) 36, 101, 182, 504, 552, 802
GluI GCNGC 3 cut(s) 630, 708, 711
GsuI CTGGAG 1 cut(s) 286
HgaI GACGC 1 cut(s) 530
Hin1II CATG 5 cut(s) 292, 318, 326, 550, 629
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HindIII AAGCTT 1 cut(s) 791
HinfI GANTC 4 cut(s) 156, 229, 242, 731
Hpy166II GTNNAC 3 cut(s) 148, 379, 650
Hpy188I TCNGA 3 cut(s) 286, 501, 798
Hpy188III TCNNGA 1 cut(s) 21
Hpy8I GTNNAC 3 cut(s) 148, 379, 650
HpyAV CCTTC 3 cut(s) 136, 490, 671
HpyCH4V TGCA 3 cut(s) 10, 434, 629
HpyF10VI GCNNNNNNNGC 2 cut(s) 521, 561
HpyF3I CTNAG 1 cut(s) 398
Hsp92II CATG 5 cut(s) 292, 318, 326, 550, 629
Kzo9I GATC 1 cut(s) 23
LguI GCTCTTC 2 cut(s) 654, 767
LpnPI CCDG 8 cut(s) 61, 88, 117, 150, 316, 444, 474, 774
Lsp1109I GCAGC 3 cut(s) 641, 694, 697
LweI GCATC 1 cut(s) 641
MaeI CTAG 6 cut(s) 36, 101, 182, 504, 552, 802
MaeIII GTNAC 1 cut(s) 577
MalI GATC 1 cut(s) 25
MboI GATC 1 cut(s) 23
MboII GAAGA 4 cut(s) 57, 265, 671, 784
MflI RGATCY 1 cut(s) 23
MhlI GDGCHC 1 cut(s) 747
MluCI AATT 7 cut(s) 189, 211, 490, 672, 717, 738, 766
MlyI GAGTC 2 cut(s) 251, 725
MnlI CCTC 4 cut(s) 37, 100, 638, 697
MseI TTAA 3 cut(s) 221, 372, 405
MspR9I CCNGG 1 cut(s) 76
MvaI CCWGG 1 cut(s) 76
MwoI GCNNNNNNNGC 2 cut(s) 521, 561
NdeII GATC 1 cut(s) 23
NheI GCTAGC 1 cut(s) 551
NlaIII CATG 5 cut(s) 292, 318, 326, 550, 629
NlaIV GGNNCC 2 cut(s) 80, 320
NmuCI GTSAC 1 cut(s) 577
PaqCI CACCTGC 1 cut(s) 469
PciSI GCTCTTC 2 cut(s) 654, 767
PfeI GAWTC 2 cut(s) 156, 229
PflMI CCANNNNNTGG 1 cut(s) 215
PkrI GCNGC 3 cut(s) 631, 709, 712
PleI GAGTC 2 cut(s) 250, 725
PpsI GAGTC 2 cut(s) 250, 725
Psp124BI GAGCTC 1 cut(s) 747
Psp6I CCWGG 1 cut(s) 74
PspGI CCWGG 1 cut(s) 74
PspN4I GGNNCC 2 cut(s) 80, 320
PspPI GGNCC 2 cut(s) 78, 319
PstI CTGCAG 1 cut(s) 436
PstNI CAGNNNCTG 1 cut(s) 440
PsuI RGATCY 1 cut(s) 23
RsaI GTAC 2 cut(s) 472, 612
RsaNI GTAC 2 cut(s) 471, 611
SacI GAGCTC 1 cut(s) 747
SapI GCTCTTC 2 cut(s) 654, 767
SaqAI TTAA 3 cut(s) 221, 372, 405
SatI GCNGC 3 cut(s) 630, 708, 711
Sau3AI GATC 1 cut(s) 23
Sau96I GGNCC 2 cut(s) 78, 319
ScaI AGTACT 1 cut(s) 612
SchI GAGTC 2 cut(s) 251, 725
ScrFI CCNGG 1 cut(s) 76
SduI GDGCHC 1 cut(s) 747
SfaNI GCATC 1 cut(s) 641
SfcI CTRYAG 1 cut(s) 432
SinI GGWCC 2 cut(s) 78, 319
SmlI CTYRAG 2 cut(s) 19, 701
SmoI CTYRAG 2 cut(s) 19, 701
Sse9I AATT 7 cut(s) 189, 211, 490, 672, 717, 738, 766
SspI AATATT 1 cut(s) 448
SspMI CTAG 6 cut(s) 36, 101, 182, 504, 552, 802
SstI GAGCTC 1 cut(s) 747
StyD4I CCNGG 1 cut(s) 74
TaqI TCGA 1 cut(s) 657
TasI AATT 7 cut(s) 189, 211, 490, 672, 717, 738, 766
TatI WGTACW 1 cut(s) 610
TfiI GAWTC 2 cut(s) 156, 229
Tru1I TTAA 3 cut(s) 221, 372, 405
Tru9I TTAA 3 cut(s) 221, 372, 405
TscAI CASTG 1 cut(s) 310
TseFI GTSAC 1 cut(s) 577
TseI GCWGC 3 cut(s) 629, 707, 710
Tsp45I GTSAC 1 cut(s) 577
TspDTI ATGAA 5 cut(s) 339, 403, 407, 617, 683
TspRI CASTG 1 cut(s) 310
Van91I CCANNNNNTGG 1 cut(s) 215
VpaK11BI GGWCC 2 cut(s) 78, 319
XcmI CCANNNNNNNNNTGG 2 cut(s) 72, 589
XspI CTAG 6 cut(s) 36, 101, 182, 504, 552, 802
ZrmI AGTACT 1 cut(s) 612
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.