Rh3BG333400
MYB Family

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0001154
Physical Location & Seq
Forward (+)
35175831 .. 35181448
5618 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG333400.1

Sequence Viewer

Length: 894 bp
ATGAGTTCAGCAAATTCTACTCGTAGAAGAAAACAACCAGAACAGCCATCACACAACTGGACCCCTCGTGAAGATGTGGCTCTAGTTGAGGCGCTAACAGAGATATGTGTTGGTGGCTATTGGAAGGTTGACAATGGCTTCAGGTCTGGGTACTTGGGTAAACTTGAACAGATTATAGAGCAAAAGCTACCAAACTGTGGGCTTAAGGCAAACCCCCACATTGAGTCTCATTTGAAGACATTGAAGAAACAAACACTTGCCATCTACGACATGCTTTCTAACTCAAGTGGGTTCTCATGGAACCATGAAGAAAAAATGGTGGTGTGTGAAAAACAAGTGTTTGATGATTGGGTTAAAGTTCATAAGGATGCAAAAGGACTGAGAATGAAACCATTCATTCATTATGATAGCTTGATAGAAGCCTTTGGCAGAGACCATGCAAATGGATTAGGAGCTGAAGGACCAGCTGAAGCAGATGAAGATCTCAATAATGAGAATAACCATGCTGACATTGAAGAAAATGATTTAGATGAAATCATAGTTCCTTCATCTGTTACCCGTAATTCTGCAAACCCACCTTCAGCTCGAACTAGGAAGCGAGCACGAGATGCTTATTCTCAATGTTTGGGAGACATGGCTAGCACATTACGCTCCTTTGTGGACGTGACCAAGGCACACTTGGAGACAATGCAGGGAGTACTCATGAATGAGCATGAAACATCTGAAAGAAGAGGTAAGCTTGTGGAGGAGCTTATGAAAATTGAAGGCATAAATGACTATGATATAATAGAAGCTACTGCTGCAATTATTGGTGATGACTCCAAAATTGACCTCTTGTTCAGTTTGCCAGACAACCTCAAAAGTCAATGGATTCATAAACTTCTTGGCCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4 Myb_DNA-bind_3

Protein Analysis

297

Amino Acids

33.51

Weight (kDa)

5.51

Isoelectric Point (pI)

54.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-bind_3 PF12776 19 - 115 3.4e-07 Myb/SANT-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 197
AcoI YGGCCR 1 cut(s) 886
AcsI RAATTY 1 cut(s) 13
AcuI CTGAAG 4 cut(s) 124, 477, 489, 564
AfaI GTAC 2 cut(s) 152, 699
AfiI CCNNNNNNNGG 1 cut(s) 197
AflII CTTAAG 1 cut(s) 203
AgsI TTSAA 5 cut(s) 167, 235, 244, 515, 764
AjiI CACGTC 1 cut(s) 664
AluBI AGCT 8 cut(s) 187, 411, 455, 467, 584, 739, 751, 794
AluI AGCT 8 cut(s) 187, 411, 455, 467, 584, 739, 751, 794
Alw21I GWGCWC 1 cut(s) 604
Alw26I GTCTC 4 cut(s) 231, 426, 624, 677
AoxI GGCC 1 cut(s) 886
ApeKI GCWGC 1 cut(s) 800
ApoI RAATTY 1 cut(s) 13
Asp700I GAANNNNTTC 1 cut(s) 392
AspLEI GCGC 1 cut(s) 94
AspS9I GGNCC 2 cut(s) 60, 461
AsuHPI GGTGA 1 cut(s) 824
AsuNHI GCTAGC 1 cut(s) 638
AvaII GGWCC 2 cut(s) 60, 461
BaeI ACNNNNGTAYC 2 cut(s) 142, 175
BauI CACGAG 2 cut(s) 66, 603
BbsI GAAGAC 1 cut(s) 242
Bbv12I GWGCWC 1 cut(s) 604
BbvI GCAGC 1 cut(s) 787
BccI CCATC 2 cut(s) 55, 269
BceAI ACGGC 1 cut(s) 873
BcoDI GTCTC 4 cut(s) 231, 426, 624, 677
BfaI CTAG 3 cut(s) 83, 591, 639
BfoI RGCGCY 1 cut(s) 95
BfrI CTTAAG 1 cut(s) 203
BglII AGATCT 1 cut(s) 481
BisI GCNGC 1 cut(s) 801
BlsI GCNGC 1 cut(s) 802
BmcAI AGTACT 1 cut(s) 699
Bme18I GGWCC 2 cut(s) 60, 461
BmgBI CACGTC 1 cut(s) 664
BmgT120I GGNCC 2 cut(s) 60, 461
BmiI GGNNCC 2 cut(s) 62, 302
BmsI GCATC 2 cut(s) 358, 598
BmtI GCTAGC 1 cut(s) 642
BpiI GAAGAC 1 cut(s) 242
BpuEI CTTGAG 1 cut(s) 268
BsaBI GATNNNNATC 1 cut(s) 480
BsaI GGTCTC 1 cut(s) 426
BsaJI CCNNGG 1 cut(s) 669
Bsc4I CCNNNNNNNGG 1 cut(s) 197
Bse1I ACTGG 1 cut(s) 62
Bse8I GATNNNNATC 1 cut(s) 480
BseDI CCNNGG 1 cut(s) 669
BseGI GGATG 1 cut(s) 373
BseJI GATNNNNATC 1 cut(s) 480
BseLI CCNNNNNNNGG 1 cut(s) 197
BseMII CTCAG 1 cut(s) 371
BseNI ACTGG 1 cut(s) 62
BseRI GAGGAG 1 cut(s) 761
BseXI GCAGC 1 cut(s) 787
BshFI GGCC 1 cut(s) 888
BsiHKAI GWGCWC 1 cut(s) 604
BslI CCNNNNNNNGG 1 cut(s) 197
BsmAI GTCTC 4 cut(s) 231, 426, 624, 677
BsnI GGCC 1 cut(s) 888
Bso31I GGTCTC 1 cut(s) 426
Bsp1286I GDGCHC 1 cut(s) 604
Bsp143I GATC 1 cut(s) 481
BspANI GGCC 1 cut(s) 888
BspCNI CTCAG 1 cut(s) 372
BspHI TCATGA 1 cut(s) 702
BspLI GGNNCC 2 cut(s) 62, 302
BspOI GCTAGC 1 cut(s) 642
BspTI CTTAAG 1 cut(s) 203
BspTNI GGTCTC 1 cut(s) 426
BsrI ACTGG 1 cut(s) 62
BssECI CCNNGG 1 cut(s) 669
BssMI GATC 1 cut(s) 481
BssSI CACGAG 2 cut(s) 66, 603
BssT1I CCWWGG 1 cut(s) 669
Bst2BI CACGAG 2 cut(s) 66, 603
Bst4CI ACNGT 1 cut(s) 197
Bst6I CTCTTC 1 cut(s) 724
BstAFI CTTAAG 1 cut(s) 203
BstAPI GCANNNNNTGC 1 cut(s) 608
BstC8I GCNNGC 2 cut(s) 600, 640
BstDEI CTNAG 1 cut(s) 380
BstF5I GGATG 1 cut(s) 373
BstH2I RGCGCY 1 cut(s) 95
BstHHI GCGC 1 cut(s) 94
BstKTI GATC 1 cut(s) 484
BstMAI GTCTC 4 cut(s) 231, 426, 624, 677
BstMBI GATC 1 cut(s) 481
BstMWI GCNNNNNNNGC 3 cut(s) 608, 648, 800
BstNSI RCATGY 1 cut(s) 274
BstV1I GCAGC 1 cut(s) 787
BstV2I GAAGAC 1 cut(s) 242
BstX2I RGATCY 1 cut(s) 481
BstXI CCANNNNNNTGG 1 cut(s) 443
BstYI RGATCY 1 cut(s) 481
BsuRI GGCC 1 cut(s) 888
BtrI CACGTC 1 cut(s) 664
BtsCI GGATG 1 cut(s) 373
Cac8I GCNNGC 2 cut(s) 600, 640
CciI TCATGA 1 cut(s) 702
CfoI GCGC 1 cut(s) 94
Cfr13I GGNCC 2 cut(s) 60, 461
Csp6I GTAC 2 cut(s) 151, 698
CviAII CATG 8 cut(s) 271, 297, 305, 437, 503, 634, 703, 713
CviQI GTAC 2 cut(s) 151, 698
DdeI CTNAG 1 cut(s) 380
DpnI GATC 1 cut(s) 483
DpnII GATC 1 cut(s) 481
EaeI YGGCCR 1 cut(s) 886
Eam1104I CTCTTC 1 cut(s) 724
EarI CTCTTC 1 cut(s) 724
Eco130I CCWWGG 1 cut(s) 669
Eco31I GGTCTC 1 cut(s) 426
Eco47I GGWCC 2 cut(s) 60, 461
Eco57I CTGAAG 4 cut(s) 124, 477, 489, 564
EcoT14I CCWWGG 1 cut(s) 669
ErhI CCWWGG 1 cut(s) 669
FaeI CATG 8 cut(s) 274, 300, 308, 440, 506, 637, 706, 716
FalI AAGNNNNNCTT 2 cut(s) 662, 694
FatI CATG 8 cut(s) 270, 296, 304, 436, 502, 633, 702, 712
Fnu4HI GCNGC 1 cut(s) 801
FokI GGATG 1 cut(s) 380
Fsp4HI GCNGC 1 cut(s) 801
FspBI CTAG 3 cut(s) 83, 591, 639
GlaI GCGC 1 cut(s) 93
GluI GCNGC 1 cut(s) 801
HaeII RGCGCY 1 cut(s) 95
HaeIII GGCC 1 cut(s) 888
HhaI GCGC 1 cut(s) 94
Hin1II CATG 8 cut(s) 274, 300, 308, 440, 506, 637, 706, 716
Hin6I GCGC 1 cut(s) 92
HinP1I GCGC 1 cut(s) 92
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HindIII AAGCTT 1 cut(s) 737
HinfI GANTC 3 cut(s) 224, 818, 871
HphI GGTGA 1 cut(s) 824
Hpy166II GTNNAC 3 cut(s) 130, 161, 661
Hpy188I TCNGA 1 cut(s) 724
Hpy188III TCNNGA 2 cut(s) 68, 703
Hpy8I GTNNAC 3 cut(s) 130, 161, 661
HpyAV CCTTC 5 cut(s) 118, 452, 555, 588, 758
HpyCH4III ACNGT 1 cut(s) 197
HpyCH4IV ACGT 1 cut(s) 663
HpyCH4V TGCA 5 cut(s) 371, 440, 569, 691, 803
HpyF10VI GCNNNNNNNGC 3 cut(s) 608, 648, 800
HpyF3I CTNAG 1 cut(s) 380
HpySE526I ACGT 1 cut(s) 663
Hsp92II CATG 8 cut(s) 274, 300, 308, 440, 506, 637, 706, 716
HspAI GCGC 1 cut(s) 92
Kzo9I GATC 1 cut(s) 481
LmnI GCTCC 3 cut(s) 452, 656, 748
LpnPI CCDG 7 cut(s) 43, 51, 127, 132, 477, 677, 861
Lsp1109I GCAGC 1 cut(s) 787
LweI GCATC 2 cut(s) 358, 598
MaeI CTAG 3 cut(s) 83, 591, 639
MaeII ACGT 1 cut(s) 663
MaeIII GTNAC 2 cut(s) 553, 664
MalI GATC 1 cut(s) 483
MboI GATC 1 cut(s) 481
MboII GAAGA 8 cut(s) 39, 83, 247, 256, 320, 491, 527, 741
MflI RGATCY 1 cut(s) 481
MhlI GDGCHC 1 cut(s) 604
MluCI AATT 5 cut(s) 13, 562, 759, 804, 825
MlyI GAGTC 2 cut(s) 233, 812
MnlI CCTC 6 cut(s) 75, 82, 725, 739, 842, 866
MroXI GAANNNNTTC 1 cut(s) 392
MseI TTAA 2 cut(s) 204, 354
MslI CAYNNNNRTG 2 cut(s) 366, 441
MspA1I CMGCKG 1 cut(s) 467
MspCI CTTAAG 1 cut(s) 203
MwoI GCNNNNNNNGC 3 cut(s) 608, 648, 800
NdeII GATC 1 cut(s) 481
NheI GCTAGC 1 cut(s) 638
NlaIII CATG 8 cut(s) 274, 300, 308, 440, 506, 637, 706, 716
NlaIV GGNNCC 2 cut(s) 62, 302
NmuCI GTSAC 1 cut(s) 664
NspI RCATGY 1 cut(s) 274
PagI TCATGA 1 cut(s) 702
PdmI GAANNNNTTC 1 cut(s) 392
PfeI GAWTC 1 cut(s) 871
PflMI CCANNNNNTGG 1 cut(s) 197
PkrI GCNGC 1 cut(s) 802
PleI GAGTC 2 cut(s) 232, 812
PpsI GAGTC 2 cut(s) 232, 812
PspN4I GGNNCC 2 cut(s) 62, 302
PspPI GGNCC 2 cut(s) 60, 461
PsuI RGATCY 1 cut(s) 481
PvuII CAGCTG 1 cut(s) 467
RsaI GTAC 2 cut(s) 152, 699
RsaNI GTAC 2 cut(s) 151, 698
RseI CAYNNNNRTG 2 cut(s) 366, 441
SaqAI TTAA 2 cut(s) 204, 354
SatI GCNGC 1 cut(s) 801
Sau3AI GATC 1 cut(s) 481
Sau96I GGNCC 2 cut(s) 60, 461
ScaI AGTACT 1 cut(s) 699
SchI GAGTC 2 cut(s) 233, 812
SduI GDGCHC 1 cut(s) 604
SfaNI GCATC 2 cut(s) 358, 598
SinI GGWCC 2 cut(s) 60, 461
SmiMI CAYNNNNRTG 2 cut(s) 366, 441
SmlI CTYRAG 2 cut(s) 203, 283
SmoI CTYRAG 2 cut(s) 203, 283
Sse9I AATT 5 cut(s) 13, 562, 759, 804, 825
SspMI CTAG 3 cut(s) 83, 591, 639
StyI CCWWGG 1 cut(s) 669
TaaI ACNGT 1 cut(s) 197
TaiI ACGT 1 cut(s) 666
TaqI TCGA 1 cut(s) 586
TasI AATT 5 cut(s) 13, 562, 759, 804, 825
TatI WGTACW 1 cut(s) 697
TfiI GAWTC 1 cut(s) 871
Tru1I TTAA 2 cut(s) 204, 354
Tru9I TTAA 2 cut(s) 204, 354
TseFI GTSAC 1 cut(s) 664
TseI GCWGC 1 cut(s) 800
Tsp45I GTSAC 1 cut(s) 664
Van91I CCANNNNNTGG 1 cut(s) 197
Vha464I CTTAAG 1 cut(s) 203
VpaK11BI GGWCC 2 cut(s) 60, 461
XapI RAATTY 1 cut(s) 13
XceI RCATGY 1 cut(s) 274
XcmI CCANNNNNNNNNTGG 2 cut(s) 54, 676
XmnI GAANNNNTTC 1 cut(s) 392
XspI CTAG 3 cut(s) 83, 591, 639
ZrmI AGTACT 1 cut(s) 699
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.