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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 333 |
| AciI | CCGC | 1 cut(s) | 38 |
| AcsI | RAATTY | 1 cut(s) | 277 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 333 |
| AgsI | TTSAA | 1 cut(s) | 11 |
| AluBI | AGCT | 3 cut(s) | 79, 127, 262 |
| AluI | AGCT | 3 cut(s) | 79, 127, 262 |
| Alw21I | GWGCWC | 1 cut(s) | 81 |
| AoxI | GGCC | 1 cut(s) | 168 |
| ApeKI | GCWGC | 2 cut(s) | 41, 44 |
| ApoI | RAATTY | 1 cut(s) | 277 |
| BanII | GRGCYC | 1 cut(s) | 81 |
| Bbv12I | GWGCWC | 1 cut(s) | 81 |
| BbvI | GCAGC | 2 cut(s) | 28, 31 |
| BceAI | ACGGC | 1 cut(s) | 183 |
| BclI | TGATCA | 2 cut(s) | 208, 316 |
| BisI | GCNGC | 3 cut(s) | 39, 42, 45 |
| BlsI | GCNGC | 3 cut(s) | 40, 43, 46 |
| BmsI | GCATC | 1 cut(s) | 140 |
| BpuEI | CTTGAG | 1 cut(s) | 139 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 333 |
| BseGI | GGATG | 2 cut(s) | 155, 211 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 333 |
| BseXI | GCAGC | 2 cut(s) | 28, 31 |
| BshFI | GGCC | 1 cut(s) | 170 |
| BsiHKAI | GWGCWC | 1 cut(s) | 81 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 333 |
| BsnI | GGCC | 1 cut(s) | 170 |
| Bsp1286I | GDGCHC | 1 cut(s) | 81 |
| Bsp143I | GATC | 2 cut(s) | 208, 316 |
| BspACI | CCGC | 1 cut(s) | 38 |
| BspANI | GGCC | 1 cut(s) | 170 |
| BssMI | GATC | 2 cut(s) | 208, 316 |
| BstC8I | GCNNGC | 1 cut(s) | 36 |
| BstDEI | CTNAG | 1 cut(s) | 305 |
| BstF5I | GGATG | 2 cut(s) | 155, 211 |
| BstKTI | GATC | 2 cut(s) | 211, 319 |
| BstMBI | GATC | 2 cut(s) | 208, 316 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 44 |
| BstV1I | GCAGC | 2 cut(s) | 28, 31 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 146 |
| BsuRI | GGCC | 1 cut(s) | 170 |
| BtgZI | GCGATG | 1 cut(s) | 75 |
| BtsCI | GGATG | 2 cut(s) | 155, 211 |
| Cac8I | GCNNGC | 1 cut(s) | 36 |
| CviJI | RGCY | 8 cut(s) | 41, 79, 90, 111, 127, 170, 221, 262 |
| CviKI_1 | RGCY | 8 cut(s) | 41, 79, 90, 111, 127, 170, 221, 262 |
| DdeI | CTNAG | 1 cut(s) | 305 |
| DpnI | GATC | 2 cut(s) | 210, 318 |
| DpnII | GATC | 2 cut(s) | 208, 316 |
| DraI | TTTAAA | 1 cut(s) | 105 |
| Ecl136II | GAGCTC | 1 cut(s) | 79 |
| Eco24I | GRGCYC | 1 cut(s) | 81 |
| Eco53kI | GAGCTC | 1 cut(s) | 79 |
| EcoICRI | GAGCTC | 1 cut(s) | 79 |
| EcoT38I | GRGCYC | 1 cut(s) | 81 |
| FaiI | YATR | 5 cut(s) | 27, 218, 228, 236, 288 |
| FbaI | TGATCA | 2 cut(s) | 208, 316 |
| Fnu4HI | GCNGC | 3 cut(s) | 39, 42, 45 |
| FokI | GGATG | 2 cut(s) | 162, 218 |
| FriOI | GRGCYC | 1 cut(s) | 81 |
| Fsp4HI | GCNGC | 3 cut(s) | 39, 42, 45 |
| GluI | GCNGC | 3 cut(s) | 39, 42, 45 |
| HaeIII | GGCC | 1 cut(s) | 170 |
| HindIII | AAGCTT | 1 cut(s) | 125 |
| HinfI | GANTC | 1 cut(s) | 65 |
| Hpy188I | TCNGA | 2 cut(s) | 213, 276 |
| Hpy188III | TCNNGA | 2 cut(s) | 95, 156 |
| HpyAV | CCTTC | 2 cut(s) | 5, 101 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 44 |
| HpyF3I | CTNAG | 1 cut(s) | 305 |
| Ksp22I | TGATCA | 2 cut(s) | 208, 316 |
| Kzo9I | GATC | 2 cut(s) | 208, 316 |
| LpnPI | CCDG | 2 cut(s) | 108, 152 |
| Lsp1109I | GCAGC | 2 cut(s) | 28, 31 |
| LweI | GCATC | 1 cut(s) | 140 |
| MaeIII | GTNAC | 1 cut(s) | 292 |
| MalI | GATC | 2 cut(s) | 210, 318 |
| MboI | GATC | 2 cut(s) | 208, 316 |
| MhlI | GDGCHC | 1 cut(s) | 81 |
| MluCI | AATT | 8 cut(s) | 6, 51, 72, 100, 176, 277, 283, 340 |
| MlyI | GAGTC | 1 cut(s) | 59 |
| MmeI | TCCRAC | 1 cut(s) | 236 |
| MseI | TTAA | 7 cut(s) | 104, 132, 173, 282, 299, 339, 343 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 44 |
| NdeII | GATC | 2 cut(s) | 208, 316 |
| NmuCI | GTSAC | 1 cut(s) | 292 |
| PacI | TTAATTAA | 1 cut(s) | 343 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 333 |
| PkrI | GCNGC | 3 cut(s) | 40, 43, 46 |
| PleI | GAGTC | 1 cut(s) | 59 |
| PpsI | GAGTC | 1 cut(s) | 59 |
| Psp124BI | GAGCTC | 1 cut(s) | 81 |
| SacI | GAGCTC | 1 cut(s) | 81 |
| SaqAI | TTAA | 7 cut(s) | 104, 132, 173, 282, 299, 339, 343 |
| SatI | GCNGC | 3 cut(s) | 39, 42, 45 |
| Sau3AI | GATC | 2 cut(s) | 208, 316 |
| SchI | GAGTC | 1 cut(s) | 59 |
| SduI | GDGCHC | 1 cut(s) | 81 |
| SetI | ASST | 4 cut(s) | 16, 81, 129, 264 |
| SfaNI | GCATC | 1 cut(s) | 140 |
| SgeI | CNNG | 7 cut(s) | 47, 94, 107, 136, 151, 168, 212 |
| SmlI | CTYRAG | 1 cut(s) | 154 |
| SmoI | CTYRAG | 1 cut(s) | 154 |
| Sse9I | AATT | 8 cut(s) | 6, 51, 72, 100, 176, 277, 283, 340 |
| SsiI | CCGC | 1 cut(s) | 38 |
| SstI | GAGCTC | 1 cut(s) | 81 |
| TasI | AATT | 8 cut(s) | 6, 51, 72, 100, 176, 277, 283, 340 |
| TauI | GCSGC | 1 cut(s) | 41 |
| Tru1I | TTAA | 7 cut(s) | 104, 132, 173, 282, 299, 339, 343 |
| Tru9I | TTAA | 7 cut(s) | 104, 132, 173, 282, 299, 339, 343 |
| TseFI | GTSAC | 1 cut(s) | 292 |
| TseI | GCWGC | 2 cut(s) | 41, 44 |
| Tsp45I | GTSAC | 1 cut(s) | 292 |
| TspDTI | ATGAA | 1 cut(s) | 17 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 333 |
| XapI | RAATTY | 1 cut(s) | 277 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 143 |