RLG00000013285
MYB Family

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0001154
Physical Location & Seq
Forward (+)
29253919 .. 29259028
5110 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013285

Sequence Viewer

Length: 909 bp
ATGTTTCTGAGATGCTCCGATCTGTTAGCACAATTCTGTTCACCATTCTCCAACTGCTATGGCATGTTTGTATCACTTATGCTTTATCATGGTCTGCTCCTTTCTGAATATCTATTGGCACTTGAGGCCAAGTATGGATGCAAGCTCTGTATTGGTCCCTGCACAACAAAAGAAGAGGGGTTCTACTATGATGCATACTATGGCAGTTTGGGCTTAAATGATGAGCACTTAAAGAAAATTGAATCAGAGGTAGAGAAGGCTGTTAAGGCCAAACAGTGCTTTGAGCGCATTGAAGTATCAATCAATCAATGGAACAGGCCCTCGAGATTTTCTCTGATAACAAATTCAAGGCTTCTTCAGCCTACTGGAGGGGAAATAAAGATCATGAAAGTCTGCAGCGAGTTTATGGAATATCATATTCGGATAAAAACTATTGCAGATGAGTCTCATTTGAAGACATTGAAGAAACAAACACTTGTCATCTACGACATGCTTTCTAACTCAAGTGGATTCTCATGGAACCATGGAGAAATGGTGGTGTGTGAAAAACAAGTGTTTGATGATTGGGTTAAAGTTCATAAGGATGCAAAAGGACTAAGAATGAAACCATTCATTCATTATGATAGCTTGATAGAAGCCTTTGGCAGAGACCGTGCAAATAGATTAGGAGCTGAAAGACCAGCGGAAGCAGATGAAGATCTCATCAATGAGAATAACTATGCTGACATTGAAGAAAATGGTTTGGATGAAATCACAGTTCCTTCATTTGTTACTCGTAATTCTGCAAACCCACCTTCAGCTCGAACTAGGAAGCGAGCACGAGATGCTTATTCTCAATGTTTGGGAGACATGGCTAGCACATTACGCTCCTTTGTGGACGTGACCAAGGCACACTTGGAGACAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4 Myb_DNA-bind_3

Protein Analysis

303

Amino Acids

34.53

Weight (kDa)

6.1

Isoelectric Point (pI)

44.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 683
AcsI RAATTY 1 cut(s) 343
AcuI CTGAAG 2 cut(s) 341, 780
AfiI CCNNNNNNNGG 1 cut(s) 368
AgsI TTSAA 6 cut(s) 242, 293, 348, 454, 463, 731
AjiI CACGTC 1 cut(s) 880
AluBI AGCT 4 cut(s) 145, 627, 671, 800
AluI AGCT 4 cut(s) 145, 627, 671, 800
Alw21I GWGCWC 2 cut(s) 228, 820
Alw26I GTCTC 4 cut(s) 450, 642, 840, 893
Ama87I CYCGRG 1 cut(s) 322
AoxI GGCC 3 cut(s) 126, 267, 317
ApeKI GCWGC 1 cut(s) 396
ApoI RAATTY 1 cut(s) 343
Asp700I GAANNNNTTC 1 cut(s) 608
AspLEI GCGC 1 cut(s) 288
AspS9I GGNCC 2 cut(s) 155, 318
AsuHPI GGTGA 1 cut(s) 33
AsuNHI GCTAGC 1 cut(s) 854
AvaI CYCGRG 1 cut(s) 322
AvaII GGWCC 1 cut(s) 155
BauI CACGAG 1 cut(s) 819
BbsI GAAGAC 1 cut(s) 461
Bbv12I GWGCWC 2 cut(s) 228, 820
BbvI GCAGC 1 cut(s) 408
BcoDI GTCTC 4 cut(s) 450, 642, 840, 893
BfaI CTAG 2 cut(s) 807, 855
BfmI CTRYAG 1 cut(s) 394
BglII AGATCT 1 cut(s) 697
BisI GCNGC 1 cut(s) 397
BlsI GCNGC 1 cut(s) 398
Bme18I GGWCC 1 cut(s) 155
BmeT110I CYCGRG 1 cut(s) 322
BmgBI CACGTC 1 cut(s) 880
BmgT120I GGNCC 2 cut(s) 155, 318
BmiI GGNNCC 2 cut(s) 157, 521
BmsI GCATC 5 cut(s) 2, 128, 181, 574, 814
BmtI GCTAGC 1 cut(s) 858
BpiI GAAGAC 1 cut(s) 461
BplI GAGNNNNNCTC 2 cut(s) 316, 348
BpmI CTGGAG 1 cut(s) 387
BpuEI CTTGAG 2 cut(s) 143, 487
BsaBI GATNNNNATC 1 cut(s) 696
BsaI GGTCTC 1 cut(s) 642
BsaJI CCNNGG 2 cut(s) 523, 885
BsaXI ACNNNNNCTCC 3 cut(s) 519, 549, 890
Bsc4I CCNNNNNNNGG 1 cut(s) 368
Bse1I ACTGG 1 cut(s) 370
Bse8I GATNNNNATC 1 cut(s) 696
BseDI CCNNGG 2 cut(s) 523, 885
BseGI GGATG 3 cut(s) 143, 589, 751
BseJI GATNNNNATC 1 cut(s) 696
BseLI CCNNNNNNNGG 1 cut(s) 368
BseNI ACTGG 1 cut(s) 370
BseXI GCAGC 1 cut(s) 408
BsgI GTGCAG 1 cut(s) 145
BshFI GGCC 3 cut(s) 128, 269, 319
BsiHKAI GWGCWC 2 cut(s) 228, 820
BsiHKCI CYCGRG 1 cut(s) 322
BslFI GGGAC 1 cut(s) 141
BslI CCNNNNNNNGG 1 cut(s) 368
BsmAI GTCTC 4 cut(s) 450, 642, 840, 893
BsmFI GGGAC 1 cut(s) 141
BsnI GGCC 3 cut(s) 128, 269, 319
Bso31I GGTCTC 1 cut(s) 642
BsoBI CYCGRG 1 cut(s) 322
Bsp1286I GDGCHC 2 cut(s) 228, 820
Bsp143I GATC 3 cut(s) 19, 381, 697
Bsp19I CCATGG 1 cut(s) 523
BspACI CCGC 1 cut(s) 683
BspANI GGCC 3 cut(s) 128, 269, 319
BspHI TCATGA 1 cut(s) 384
BspLI GGNNCC 2 cut(s) 157, 521
BspMAI CTGCAG 1 cut(s) 398
BspOI GCTAGC 1 cut(s) 858
BspTNI GGTCTC 1 cut(s) 642
BsrI ACTGG 1 cut(s) 370
BssECI CCNNGG 2 cut(s) 523, 885
BssMI GATC 3 cut(s) 19, 381, 697
BssSI CACGAG 1 cut(s) 819
BssT1I CCWWGG 2 cut(s) 523, 885
Bst2BI CACGAG 1 cut(s) 819
Bst4CI ACNGT 3 cut(s) 276, 653, 757
Bst6I CTCTTC 1 cut(s) 168
BstAPI GCANNNNNTGC 1 cut(s) 824
BstC8I GCNNGC 3 cut(s) 143, 816, 856
BstDEI CTNAG 2 cut(s) 8, 596
BstDSI CCRYGG 1 cut(s) 523
BstENI CCTNNNNNAGG 1 cut(s) 366
BstF5I GGATG 3 cut(s) 143, 589, 751
BstHHI GCGC 1 cut(s) 288
BstKTI GATC 3 cut(s) 22, 384, 700
BstMAI GTCTC 4 cut(s) 450, 642, 840, 893
BstMBI GATC 3 cut(s) 19, 381, 697
BstMWI GCNNNNNNNGC 7 cut(s) 125, 210, 266, 285, 358, 824, 864
BstNSI RCATGY 2 cut(s) 67, 493
BstSFI CTRYAG 1 cut(s) 394
BstV1I GCAGC 1 cut(s) 408
BstV2I GAAGAC 1 cut(s) 461
BstX2I RGATCY 1 cut(s) 697
BstYI RGATCY 1 cut(s) 697
BsuRI GGCC 3 cut(s) 128, 269, 319
BtgI CCRYGG 1 cut(s) 523
BtrI CACGTC 1 cut(s) 880
BtsCI GGATG 3 cut(s) 143, 589, 751
BtsIMutI CAGTG 1 cut(s) 281
Cac8I GCNNGC 3 cut(s) 143, 816, 856
CciI TCATGA 1 cut(s) 384
CfoI GCGC 1 cut(s) 288
Cfr13I GGNCC 2 cut(s) 155, 318
CviAII CATG 7 cut(s) 64, 89, 385, 490, 516, 524, 850
DdeI CTNAG 2 cut(s) 8, 596
DpnI GATC 3 cut(s) 21, 383, 699
DpnII GATC 3 cut(s) 19, 381, 697
Eam1104I CTCTTC 1 cut(s) 168
EarI CTCTTC 1 cut(s) 168
Eco130I CCWWGG 2 cut(s) 523, 885
Eco31I GGTCTC 1 cut(s) 642
Eco47I GGWCC 1 cut(s) 155
Eco57I CTGAAG 2 cut(s) 341, 780
Eco88I CYCGRG 1 cut(s) 322
EcoNI CCTNNNNNAGG 1 cut(s) 366
EcoO109I RGGNCCY 1 cut(s) 318
EcoT14I CCWWGG 2 cut(s) 523, 885
EcoT22I ATGCAT 1 cut(s) 196
ErhI CCWWGG 2 cut(s) 523, 885
FaeI CATG 7 cut(s) 67, 92, 388, 493, 519, 527, 853
FalI AAGNNNNNCTT 1 cut(s) 878
FaqI GGGAC 1 cut(s) 141
FatI CATG 7 cut(s) 63, 88, 384, 489, 515, 523, 849
Fnu4HI GCNGC 1 cut(s) 397
FokI GGATG 3 cut(s) 150, 596, 758
Fsp4HI GCNGC 1 cut(s) 397
FspBI CTAG 2 cut(s) 807, 855
GlaI GCGC 1 cut(s) 287
GluI GCNGC 1 cut(s) 397
GsuI CTGGAG 1 cut(s) 387
HaeIII GGCC 3 cut(s) 128, 269, 319
HhaI GCGC 1 cut(s) 288
Hin1II CATG 7 cut(s) 67, 92, 388, 493, 519, 527, 853
Hin6I GCGC 1 cut(s) 286
HinP1I GCGC 1 cut(s) 286
HinfI GANTC 3 cut(s) 242, 443, 510
HphI GGTGA 1 cut(s) 33
Hpy166II GTNNAC 2 cut(s) 41, 877
Hpy188I TCNGA 6 cut(s) 9, 19, 106, 247, 336, 423
Hpy188III TCNNGA 2 cut(s) 324, 385
Hpy8I GTNNAC 2 cut(s) 41, 877
HpyAV CCTTC 3 cut(s) 250, 771, 804
HpyCH4III ACNGT 3 cut(s) 276, 653, 757
HpyCH4IV ACGT 1 cut(s) 879
HpyCH4V TGCA 8 cut(s) 141, 162, 194, 396, 437, 587, 656, 785
HpyF10VI GCNNNNNNNGC 7 cut(s) 125, 210, 266, 285, 358, 824, 864
HpyF3I CTNAG 2 cut(s) 8, 596
HpySE526I ACGT 1 cut(s) 879
Hsp92II CATG 7 cut(s) 67, 92, 388, 493, 519, 527, 853
HspAI GCGC 1 cut(s) 286
Kzo9I GATC 3 cut(s) 19, 381, 697
LmnI GCTCC 4 cut(s) 20, 102, 668, 872
LpnPI CCDG 4 cut(s) 172, 301, 351, 693
Lsp1109I GCAGC 1 cut(s) 408
LweI GCATC 5 cut(s) 2, 128, 181, 574, 814
MaeI CTAG 2 cut(s) 807, 855
MaeII ACGT 1 cut(s) 879
MaeIII GTNAC 2 cut(s) 769, 880
MalI GATC 3 cut(s) 21, 383, 699
MboI GATC 3 cut(s) 19, 381, 697
MboII GAAGA 6 cut(s) 185, 347, 466, 475, 707, 743
MflI RGATCY 1 cut(s) 697
MhlI GDGCHC 2 cut(s) 228, 820
MluCI AATT 4 cut(s) 32, 237, 343, 778
MlyI GAGTC 1 cut(s) 452
MmeI TCCRAC 1 cut(s) 75
MnlI CCTC 5 cut(s) 118, 169, 241, 331, 362
Mph1103I ATGCAT 1 cut(s) 196
MroXI GAANNNNTTC 1 cut(s) 608
MseI TTAA 4 cut(s) 215, 230, 264, 570
MslI CAYNNNNRTG 1 cut(s) 582
MspA1I CMGCKG 1 cut(s) 683
MwoI GCNNNNNNNGC 7 cut(s) 125, 210, 266, 285, 358, 824, 864
NcoI CCATGG 1 cut(s) 523
NdeII GATC 3 cut(s) 19, 381, 697
NheI GCTAGC 1 cut(s) 854
NlaIII CATG 7 cut(s) 67, 92, 388, 493, 519, 527, 853
NlaIV GGNNCC 2 cut(s) 157, 521
NmuCI GTSAC 1 cut(s) 880
NsiI ATGCAT 1 cut(s) 196
NspI RCATGY 2 cut(s) 67, 493
PaeR7I CTCGAG 1 cut(s) 322
PagI TCATGA 1 cut(s) 384
PdmI GAANNNNTTC 1 cut(s) 608
PfeI GAWTC 2 cut(s) 242, 510
PkrI GCNGC 1 cut(s) 398
PleI GAGTC 1 cut(s) 451
PpsI GAGTC 1 cut(s) 451
PspN4I GGNNCC 2 cut(s) 157, 521
PspPI GGNCC 2 cut(s) 155, 318
PstI CTGCAG 1 cut(s) 398
PsuI RGATCY 1 cut(s) 697
RseI CAYNNNNRTG 1 cut(s) 582
SaqAI TTAA 4 cut(s) 215, 230, 264, 570
SatI GCNGC 1 cut(s) 397
Sau3AI GATC 3 cut(s) 19, 381, 697
Sau96I GGNCC 2 cut(s) 155, 318
SchI GAGTC 1 cut(s) 452
SduI GDGCHC 2 cut(s) 228, 820
SetI ASST 7 cut(s) 147, 252, 629, 673, 796, 802, 882
SfaNI GCATC 5 cut(s) 2, 128, 181, 574, 814
SfcI CTRYAG 1 cut(s) 394
Sfr274I CTCGAG 1 cut(s) 322
SinI GGWCC 1 cut(s) 155
SlaI CTCGAG 1 cut(s) 322
SmiMI CAYNNNNRTG 1 cut(s) 582
SmlI CTYRAG 3 cut(s) 122, 322, 502
SmoI CTYRAG 3 cut(s) 122, 322, 502
Sse9I AATT 4 cut(s) 32, 237, 343, 778
SsiI CCGC 1 cut(s) 683
SspMI CTAG 2 cut(s) 807, 855
StyI CCWWGG 2 cut(s) 523, 885
TaaI ACNGT 3 cut(s) 276, 653, 757
TaiI ACGT 1 cut(s) 882
TaqI TCGA 2 cut(s) 323, 802
TasI AATT 4 cut(s) 32, 237, 343, 778
TfiI GAWTC 2 cut(s) 242, 510
Tru1I TTAA 4 cut(s) 215, 230, 264, 570
Tru9I TTAA 4 cut(s) 215, 230, 264, 570
TscAI CASTG 1 cut(s) 281
TseFI GTSAC 1 cut(s) 880
TseI GCWGC 1 cut(s) 396
Tsp45I GTSAC 1 cut(s) 880
TspDTI ATGAA 8 cut(s) 401, 566, 601, 605, 617, 708, 753, 762
TspRI CASTG 1 cut(s) 281
VpaK11BI GGWCC 1 cut(s) 155
XagI CCTNNNNNAGG 1 cut(s) 366
XapI RAATTY 1 cut(s) 343
XceI RCATGY 2 cut(s) 67, 493
XcmI CCANNNNNNNNNTGG 1 cut(s) 892
XhoI CTCGAG 1 cut(s) 322
XmnI GAANNNNTTC 1 cut(s) 608
XspI CTAG 2 cut(s) 807, 855
Zsp2I ATGCAT 1 cut(s) 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.