nuclease HARBI1
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcuI | CTGAAG | 3 cut(s) | 207, 219, 294 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 173 |
| AgsI | TTSAA | 1 cut(s) | 245 |
| AjiI | CACGTC | 2 cut(s) | 364, 394 |
| AluBI | AGCT | 4 cut(s) | 141, 185, 197, 314 |
| AluI | AGCT | 4 cut(s) | 141, 185, 197, 314 |
| Alw26I | GTCTC | 3 cut(s) | 156, 354, 407 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 122 |
| AspS9I | GGNCC | 1 cut(s) | 191 |
| AsuNHI | GCTAGC | 1 cut(s) | 368 |
| AvaII | GGWCC | 1 cut(s) | 191 |
| BauI | CACGAG | 1 cut(s) | 333 |
| BcoDI | GTCTC | 3 cut(s) | 156, 354, 407 |
| BfaI | CTAG | 2 cut(s) | 321, 369 |
| BglII | AGATCT | 1 cut(s) | 211 |
| Bme18I | GGWCC | 1 cut(s) | 191 |
| BmgBI | CACGTC | 2 cut(s) | 364, 394 |
| BmgT120I | GGNCC | 1 cut(s) | 191 |
| BmiI | GGNNCC | 1 cut(s) | 32 |
| BmsI | GCATC | 2 cut(s) | 88, 328 |
| BmtI | GCTAGC | 1 cut(s) | 372 |
| BpuEI | CTTGAG | 1 cut(s) | 333 |
| BsaBI | GATNNNNATC | 1 cut(s) | 210 |
| BsaI | GGTCTC | 1 cut(s) | 156 |
| BsaJI | CCNNGG | 1 cut(s) | 399 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 173 |
| Bse8I | GATNNNNATC | 1 cut(s) | 210 |
| BseDI | CCNNGG | 1 cut(s) | 399 |
| BseGI | GGATG | 2 cut(s) | 103, 265 |
| BseJI | GATNNNNATC | 1 cut(s) | 210 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 173 |
| BseMII | CTCAG | 1 cut(s) | 101 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 173 |
| BsmAI | GTCTC | 3 cut(s) | 156, 354, 407 |
| BsmBI | CGTCTC | 1 cut(s) | 354 |
| Bso31I | GGTCTC | 1 cut(s) | 156 |
| Bsp143I | GATC | 1 cut(s) | 211 |
| Bsp19I | CCATGG | 1 cut(s) | 399 |
| BspCNI | CTCAG | 1 cut(s) | 102 |
| BspLI | GGNNCC | 1 cut(s) | 32 |
| BspOI | GCTAGC | 1 cut(s) | 372 |
| BspTNI | GGTCTC | 1 cut(s) | 156 |
| BssECI | CCNNGG | 1 cut(s) | 399 |
| BssMI | GATC | 1 cut(s) | 211 |
| BssSI | CACGAG | 1 cut(s) | 333 |
| BssT1I | CCWWGG | 1 cut(s) | 399 |
| Bst2BI | CACGAG | 1 cut(s) | 333 |
| Bst4CI | ACNGT | 1 cut(s) | 271 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 338 |
| BstC8I | GCNNGC | 2 cut(s) | 330, 370 |
| BstDEI | CTNAG | 1 cut(s) | 110 |
| BstDSI | CCRYGG | 1 cut(s) | 399 |
| BstF5I | GGATG | 2 cut(s) | 103, 265 |
| BstKTI | GATC | 1 cut(s) | 214 |
| BstMAI | GTCTC | 3 cut(s) | 156, 354, 407 |
| BstMBI | GATC | 1 cut(s) | 211 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 338, 378 |
| BstX2I | RGATCY | 1 cut(s) | 211 |
| BstYI | RGATCY | 1 cut(s) | 211 |
| BtgI | CCRYGG | 1 cut(s) | 399 |
| BtrI | CACGTC | 2 cut(s) | 364, 394 |
| BtsCI | GGATG | 2 cut(s) | 103, 265 |
| Cac8I | GCNNGC | 2 cut(s) | 330, 370 |
| Cfr13I | GGNCC | 1 cut(s) | 191 |
| CviAII | CATG | 3 cut(s) | 27, 35, 400 |
| CviJI | RGCY | 6 cut(s) | 141, 152, 185, 197, 314, 368 |
| CviKI_1 | RGCY | 6 cut(s) | 141, 152, 185, 197, 314, 368 |
| DdeI | CTNAG | 1 cut(s) | 110 |
| DpnI | GATC | 1 cut(s) | 213 |
| DpnII | GATC | 1 cut(s) | 211 |
| Eco130I | CCWWGG | 1 cut(s) | 399 |
| Eco31I | GGTCTC | 1 cut(s) | 156 |
| Eco47I | GGWCC | 1 cut(s) | 191 |
| Eco57I | CTGAAG | 3 cut(s) | 207, 219, 294 |
| EcoT14I | CCWWGG | 1 cut(s) | 399 |
| ErhI | CCWWGG | 1 cut(s) | 399 |
| Esp3I | CGTCTC | 1 cut(s) | 354 |
| FaeI | CATG | 3 cut(s) | 30, 38, 403 |
| FaiI | YATR | 6 cut(s) | 28, 36, 93, 135, 234, 401 |
| FatI | CATG | 3 cut(s) | 26, 34, 399 |
| FokI | GGATG | 2 cut(s) | 110, 272 |
| FspBI | CTAG | 2 cut(s) | 321, 369 |
| Hin1II | CATG | 3 cut(s) | 30, 38, 403 |
| HinfI | GANTC | 1 cut(s) | 21 |
| Hpy166II | GTNNAC | 1 cut(s) | 391 |
| Hpy8I | GTNNAC | 1 cut(s) | 391 |
| HpyAV | CCTTC | 3 cut(s) | 182, 285, 318 |
| HpyCH4III | ACNGT | 1 cut(s) | 271 |
| HpyCH4IV | ACGT | 2 cut(s) | 363, 393 |
| HpyCH4V | TGCA | 4 cut(s) | 101, 170, 299, 421 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 338, 378 |
| HpyF3I | CTNAG | 1 cut(s) | 110 |
| HpySE526I | ACGT | 2 cut(s) | 363, 393 |
| Hsp92II | CATG | 3 cut(s) | 30, 38, 403 |
| Kzo9I | GATC | 1 cut(s) | 211 |
| LmnI | GCTCC | 2 cut(s) | 182, 386 |
| LpnPI | CCDG | 2 cut(s) | 180, 207 |
| LweI | GCATC | 2 cut(s) | 88, 328 |
| MaeI | CTAG | 2 cut(s) | 321, 369 |
| MaeII | ACGT | 2 cut(s) | 363, 393 |
| MaeIII | GTNAC | 2 cut(s) | 283, 394 |
| MalI | GATC | 1 cut(s) | 213 |
| MboI | GATC | 1 cut(s) | 211 |
| MboII | GAAGA | 2 cut(s) | 50, 221 |
| MflI | RGATCY | 1 cut(s) | 211 |
| MluCI | AATT | 1 cut(s) | 292 |
| MroXI | GAANNNNTTC | 1 cut(s) | 122 |
| MseI | TTAA | 1 cut(s) | 84 |
| MslI | CAYNNNNRTG | 1 cut(s) | 96 |
| MspA1I | CMGCKG | 1 cut(s) | 197 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 338, 378 |
| NcoI | CCATGG | 1 cut(s) | 399 |
| NdeII | GATC | 1 cut(s) | 211 |
| NheI | GCTAGC | 1 cut(s) | 368 |
| NlaIII | CATG | 3 cut(s) | 30, 38, 403 |
| NlaIV | GGNNCC | 1 cut(s) | 32 |
| NmuCI | GTSAC | 1 cut(s) | 394 |
| PdmI | GAANNNNTTC | 1 cut(s) | 122 |
| PfeI | GAWTC | 1 cut(s) | 21 |
| PspN4I | GGNNCC | 1 cut(s) | 32 |
| PspPI | GGNCC | 1 cut(s) | 191 |
| PsuI | RGATCY | 1 cut(s) | 211 |
| PvuII | CAGCTG | 1 cut(s) | 197 |
| RseI | CAYNNNNRTG | 1 cut(s) | 96 |
| SaqAI | TTAA | 1 cut(s) | 84 |
| Sau3AI | GATC | 1 cut(s) | 211 |
| Sau96I | GGNCC | 1 cut(s) | 191 |
| SetI | ASST | 8 cut(s) | 143, 187, 199, 310, 316, 355, 366, 396 |
| SfaNI | GCATC | 2 cut(s) | 88, 328 |
| SinI | GGWCC | 1 cut(s) | 191 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 96 |
| SmlI | CTYRAG | 2 cut(s) | 13, 348 |
| SmoI | CTYRAG | 2 cut(s) | 13, 348 |
| Sse9I | AATT | 1 cut(s) | 292 |
| SspMI | CTAG | 2 cut(s) | 321, 369 |
| StyI | CCWWGG | 1 cut(s) | 399 |
| TaaI | ACNGT | 1 cut(s) | 271 |
| TaiI | ACGT | 2 cut(s) | 366, 396 |
| TaqI | TCGA | 1 cut(s) | 316 |
| TasI | AATT | 1 cut(s) | 292 |
| TfiI | GAWTC | 1 cut(s) | 21 |
| Tru1I | TTAA | 1 cut(s) | 84 |
| Tru9I | TTAA | 1 cut(s) | 84 |
| TseFI | GTSAC | 1 cut(s) | 394 |
| Tsp45I | GTSAC | 1 cut(s) | 394 |
| TspDTI | ATGAA | 8 cut(s) | 51, 80, 115, 119, 131, 222, 267, 276 |
| VpaK11BI | GGWCC | 1 cut(s) | 191 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 406 |
| XmnI | GAANNNNTTC | 1 cut(s) | 122 |
| XspI | CTAG | 2 cut(s) | 321, 369 |