RchiOBHm_Chr6g0274461
MYB Family

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0001154
Physical Location & Seq
Forward (+)
35937340 .. 35942374
5035 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24621

Sequence Viewer

Length: 624 bp
ATGCTTTCGAACTCAAGTGGGTTCTCATGGAACCATGAAGAAAAAATGGTGGTGTGTGAAAAAAAAGTGTTTGATGATTGGGTTAAAGTTCATAAGGATGCAAAAGGACTGAGAATGAAACCATTCATTCATTATGATAGCTTGATAGAAGCCTTTGGCAGAGACCGTGCAAATGGATTAGGAGCTGAAGGACCAGCCGAAGCAGATGAGGATCTCAATAATGAGAATAACTATGCTGACATTGAAGAAAATGGTTTGGATGAAATCACAGTTCCTTCATCTGTTACCCGTAATTCTGCAAACCCACCTTCAGCTCGAACTAGGAAGCGAGCACGAGATGCTTATTCTCAATGTTTGGGAGACATGGCTAGCACATTACGCTCCTTTGTGGACGTGACCAAGGCACACTTGGAGACAATGCAGGGAGTACTCATGAATGAGCATGTAACATCTGAAAGAAGAGGTAAGCTTGTGGAGGAGCTTATGAAAATTGAAGGCATAAATGACTATGATGTAATAGAAGCTGCTGCTGCAATTATTGGTGATGACTCCAAAATTGACCTCTTGTTTAGTTTGCCAGACAACCTCAAAAGTCAATGGATTCATAAACTTCTTGGCCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4 Myb_DNA-bind_3

Protein Analysis

207

Amino Acids

23.2

Weight (kDa)

5.11

Isoelectric Point (pI)

47.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 219
AcoI YGGCCR 1 cut(s) 616
AcuI CTGAAG 2 cut(s) 207, 294
AfaI GTAC 1 cut(s) 429
AgsI TTSAA 2 cut(s) 245, 494
AjiI CACGTC 1 cut(s) 394
AluBI AGCT 6 cut(s) 141, 185, 314, 469, 481, 524
AluI AGCT 6 cut(s) 141, 185, 314, 469, 481, 524
Alw21I GWGCWC 1 cut(s) 334
Alw26I GTCTC 3 cut(s) 156, 354, 407
AlwI GGATC 1 cut(s) 219
AoxI GGCC 1 cut(s) 616
ApeKI GCWGC 3 cut(s) 524, 527, 530
Asp700I GAANNNNTTC 1 cut(s) 122
AspS9I GGNCC 1 cut(s) 191
AsuHPI GGTGA 1 cut(s) 554
AsuII TTCGAA 1 cut(s) 8
AsuNHI GCTAGC 1 cut(s) 368
AvaII GGWCC 1 cut(s) 191
BauI CACGAG 1 cut(s) 333
Bbv12I GWGCWC 1 cut(s) 334
BbvI GCAGC 3 cut(s) 511, 514, 517
BceAI ACGGC 1 cut(s) 603
BcoDI GTCTC 3 cut(s) 156, 354, 407
BfaI CTAG 2 cut(s) 321, 369
BisI GCNGC 3 cut(s) 525, 528, 531
BlsI GCNGC 3 cut(s) 526, 529, 532
BmcAI AGTACT 1 cut(s) 429
Bme18I GGWCC 1 cut(s) 191
BmgBI CACGTC 1 cut(s) 394
BmgT120I GGNCC 1 cut(s) 191
BmiI GGNNCC 1 cut(s) 32
BmsI GCATC 2 cut(s) 88, 328
BmtI GCTAGC 1 cut(s) 372
Bpu14I TTCGAA 1 cut(s) 8
BsaBI GATNNNNATC 1 cut(s) 210
BsaI GGTCTC 1 cut(s) 156
BsaJI CCNNGG 1 cut(s) 399
Bse8I GATNNNNATC 1 cut(s) 210
BseDI CCNNGG 1 cut(s) 399
BseGI GGATG 2 cut(s) 103, 265
BseJI GATNNNNATC 1 cut(s) 210
BseMII CTCAG 1 cut(s) 101
BseRI GAGGAG 1 cut(s) 491
BseXI GCAGC 3 cut(s) 511, 514, 517
BshFI GGCC 1 cut(s) 618
BsiHKAI GWGCWC 1 cut(s) 334
BsmAI GTCTC 3 cut(s) 156, 354, 407
BsnI GGCC 1 cut(s) 618
Bso31I GGTCTC 1 cut(s) 156
Bsp119I TTCGAA 1 cut(s) 8
Bsp1286I GDGCHC 1 cut(s) 334
Bsp143I GATC 1 cut(s) 211
BspANI GGCC 1 cut(s) 618
BspCNI CTCAG 1 cut(s) 102
BspHI TCATGA 1 cut(s) 432
BspLI GGNNCC 1 cut(s) 32
BspOI GCTAGC 1 cut(s) 372
BspPI GGATC 1 cut(s) 219
BspT104I TTCGAA 1 cut(s) 8
BspTNI GGTCTC 1 cut(s) 156
BssECI CCNNGG 1 cut(s) 399
BssMI GATC 1 cut(s) 211
BssSI CACGAG 1 cut(s) 333
BssT1I CCWWGG 1 cut(s) 399
Bst2BI CACGAG 1 cut(s) 333
Bst4CI ACNGT 2 cut(s) 167, 271
Bst6I CTCTTC 1 cut(s) 454
BstAPI GCANNNNNTGC 1 cut(s) 338
BstBI TTCGAA 1 cut(s) 8
BstC8I GCNNGC 2 cut(s) 330, 370
BstDEI CTNAG 1 cut(s) 110
BstF5I GGATG 2 cut(s) 103, 265
BstKTI GATC 1 cut(s) 214
BstMAI GTCTC 3 cut(s) 156, 354, 407
BstMBI GATC 1 cut(s) 211
BstMWI GCNNNNNNNGC 3 cut(s) 338, 378, 530
BstNSI RCATGY 1 cut(s) 446
BstV1I GCAGC 3 cut(s) 511, 514, 517
BstX2I RGATCY 1 cut(s) 211
BstYI RGATCY 1 cut(s) 211
BsuRI GGCC 1 cut(s) 618
BtrI CACGTC 1 cut(s) 394
BtsCI GGATG 2 cut(s) 103, 265
Cac8I GCNNGC 2 cut(s) 330, 370
CciI TCATGA 1 cut(s) 432
Cfr13I GGNCC 1 cut(s) 191
Csp6I GTAC 1 cut(s) 428
CviAII CATG 5 cut(s) 27, 35, 364, 433, 443
CviQI GTAC 1 cut(s) 428
DdeI CTNAG 1 cut(s) 110
DpnI GATC 1 cut(s) 213
DpnII GATC 1 cut(s) 211
EaeI YGGCCR 1 cut(s) 616
Eam1104I CTCTTC 1 cut(s) 454
EarI CTCTTC 1 cut(s) 454
Eco130I CCWWGG 1 cut(s) 399
Eco31I GGTCTC 1 cut(s) 156
Eco47I GGWCC 1 cut(s) 191
Eco57I CTGAAG 2 cut(s) 207, 294
EcoT14I CCWWGG 1 cut(s) 399
ErhI CCWWGG 1 cut(s) 399
FaeI CATG 5 cut(s) 30, 38, 367, 436, 446
FalI AAGNNNNNCTT 2 cut(s) 392, 424
FatI CATG 5 cut(s) 26, 34, 363, 432, 442
Fnu4HI GCNGC 3 cut(s) 525, 528, 531
FokI GGATG 2 cut(s) 110, 272
Fsp4HI GCNGC 3 cut(s) 525, 528, 531
FspBI CTAG 2 cut(s) 321, 369
GluI GCNGC 3 cut(s) 525, 528, 531
HaeIII GGCC 1 cut(s) 618
Hin1II CATG 5 cut(s) 30, 38, 367, 436, 446
HindIII AAGCTT 1 cut(s) 467
HinfI GANTC 2 cut(s) 548, 601
HphI GGTGA 1 cut(s) 554
Hpy166II GTNNAC 1 cut(s) 391
Hpy188I TCNGA 1 cut(s) 454
Hpy188III TCNNGA 1 cut(s) 433
Hpy8I GTNNAC 1 cut(s) 391
HpyAV CCTTC 4 cut(s) 182, 285, 318, 488
HpyCH4III ACNGT 2 cut(s) 167, 271
HpyCH4IV ACGT 1 cut(s) 393
HpyCH4V TGCA 5 cut(s) 101, 170, 299, 421, 533
HpyF10VI GCNNNNNNNGC 3 cut(s) 338, 378, 530
HpyF3I CTNAG 1 cut(s) 110
HpySE526I ACGT 1 cut(s) 393
Hsp92II CATG 5 cut(s) 30, 38, 367, 436, 446
Kzo9I GATC 1 cut(s) 211
LmnI GCTCC 3 cut(s) 182, 386, 478
LpnPI CCDG 3 cut(s) 207, 407, 591
Lsp1109I GCAGC 3 cut(s) 511, 514, 517
LweI GCATC 2 cut(s) 88, 328
MaeI CTAG 2 cut(s) 321, 369
MaeII ACGT 1 cut(s) 393
MaeIII GTNAC 3 cut(s) 283, 394, 445
MalI GATC 1 cut(s) 213
MboI GATC 1 cut(s) 211
MboII GAAGA 3 cut(s) 50, 257, 471
MflI RGATCY 1 cut(s) 211
MhlI GDGCHC 1 cut(s) 334
MluCI AATT 4 cut(s) 292, 489, 534, 555
MlyI GAGTC 1 cut(s) 542
MnlI CCTC 5 cut(s) 202, 455, 469, 572, 596
MroXI GAANNNNTTC 1 cut(s) 122
MseI TTAA 1 cut(s) 84
MslI CAYNNNNRTG 1 cut(s) 96
MwoI GCNNNNNNNGC 3 cut(s) 338, 378, 530
NdeII GATC 1 cut(s) 211
NheI GCTAGC 1 cut(s) 368
NlaIII CATG 5 cut(s) 30, 38, 367, 436, 446
NlaIV GGNNCC 1 cut(s) 32
NmuCI GTSAC 1 cut(s) 394
NspI RCATGY 1 cut(s) 446
NspV TTCGAA 1 cut(s) 8
PagI TCATGA 1 cut(s) 432
PdmI GAANNNNTTC 1 cut(s) 122
PfeI GAWTC 1 cut(s) 601
PkrI GCNGC 3 cut(s) 526, 529, 532
PleI GAGTC 1 cut(s) 542
PpsI GAGTC 1 cut(s) 542
PspN4I GGNNCC 1 cut(s) 32
PspPI GGNCC 1 cut(s) 191
PsuI RGATCY 1 cut(s) 211
RsaI GTAC 1 cut(s) 429
RsaNI GTAC 1 cut(s) 428
RseI CAYNNNNRTG 1 cut(s) 96
SaqAI TTAA 1 cut(s) 84
SatI GCNGC 3 cut(s) 525, 528, 531
Sau3AI GATC 1 cut(s) 211
Sau96I GGNCC 1 cut(s) 191
ScaI AGTACT 1 cut(s) 429
SchI GAGTC 1 cut(s) 542
SduI GDGCHC 1 cut(s) 334
SfaNI GCATC 2 cut(s) 88, 328
SfuI TTCGAA 1 cut(s) 8
SinI GGWCC 1 cut(s) 191
SmiMI CAYNNNNRTG 1 cut(s) 96
SmlI CTYRAG 1 cut(s) 13
SmoI CTYRAG 1 cut(s) 13
Sse9I AATT 4 cut(s) 292, 489, 534, 555
SspMI CTAG 2 cut(s) 321, 369
StyI CCWWGG 1 cut(s) 399
TaaI ACNGT 2 cut(s) 167, 271
TaiI ACGT 1 cut(s) 396
TaqI TCGA 2 cut(s) 8, 316
TasI AATT 4 cut(s) 292, 489, 534, 555
TatI WGTACW 1 cut(s) 427
TfiI GAWTC 1 cut(s) 601
Tru1I TTAA 1 cut(s) 84
Tru9I TTAA 1 cut(s) 84
TseFI GTSAC 1 cut(s) 394
TseI GCWGC 3 cut(s) 524, 527, 530
Tsp45I GTSAC 1 cut(s) 394
VpaK11BI GGWCC 1 cut(s) 191
XceI RCATGY 1 cut(s) 446
XcmI CCANNNNNNNNNTGG 1 cut(s) 406
XmnI GAANNNNTTC 1 cut(s) 122
XspI CTAG 2 cut(s) 321, 369
ZrmI AGTACT 1 cut(s) 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.