Rroxscaffold_7G00175350
MYB Family

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001154
Physical Location & Seq
Forward (+)
14915446 .. 14918611
3166 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00175350.1

Sequence Viewer

Length: 858 bp
ATGTTGACGCAACAGGCGGCGACGGCGGAGTGTGGCTTGCAGGAGGGAGGCCGGAGGCGTCCCGGACAGTTCTGGGCACCATTCGAGGTCGGAGGAGTTCGGGCTTGCCGGTTTACGGGCAGCCACCTCACCTGCAACTGCAGGATGAATTTGCAAGCAAACTCAAGATCGTCAGCTAGTAAAAGTGGCAGAAATAAAGACAAGCAGCCATCACACACATGGACCCCTATTGAAGATGTAGCTCTAGTTGAGGCGTTAACTGAACTTTGTGTTGGTGGGACCTGGAAGGTTGACAATGGATTCAAGGCAGGATATTTGGGACAACTGGAAAATATTATAGAGCAAAAGCAACCAAATTGTGGATTAAAGGCAAACCCACACATTGAGTCTCGTGTTAAGACACTAAATAAACAAACACTTGCCATCTCTGACATGATTGCTAACTCAAATGGCTTCTCATGGGACCATGAAAATAAAATGGTTGTTTGTGAAAAACAAGTTCACAAGGAAGCCAAAGGACTAAGACTTAAACCATTCATTCATTATGATACTTTAGTGGAAGCTTTTGGGAGAGATAGAGCTAATGGGTTAGGAGACATGGCTAGCACACTTCGCTCATTTGTGGATGTGAACAAGACACACTTGGAGACATTGAAAGAAGTACTTTTGAATGACCATGCAACATCTGCAAAAAGATCTAAGCTTGTCAAAGAGCTTATAAAAATTGAAGGCTTGAATGATTATGATGTGATTGAGGCTGCTGCTACAATTATTGGTGATGACTCCAAAATTGAGCTCTTGTTCAGCTTACCTGACAGTTTAAAGAGCCGGTGGATTTACTGGCTTCTTAACCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4 Myb_DNA-bind_3

Protein Analysis

285

Amino Acids

31.79

Weight (kDa)

8.41

Isoelectric Point (pI)

55.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-bind_3 PF12776 73 - 167 6.8e-07 Myb/SANT-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 719
AarI CACCTGC 1 cut(s) 140
Acc36I ACCTGC 1 cut(s) 140
AccB1I GGYRCC 1 cut(s) 76
AccB7I CCANNNNNTGG 1 cut(s) 359
AciI CCGC 2 cut(s) 17, 26
AcsI RAATTY 1 cut(s) 148
AcyI GRCGYC 1 cut(s) 58
AfaI GTAC 1 cut(s) 663
AfiI CCNNNNNNNGG 2 cut(s) 115, 359
AgsI TTSAA 6 cut(s) 233, 304, 655, 670, 728, 736
AjnI CCWGG 1 cut(s) 281
AjuI GAANNNNNNNTTGG 2 cut(s) 255, 287
AluBI AGCT 8 cut(s) 176, 242, 563, 581, 703, 715, 796, 807
AluI AGCT 8 cut(s) 176, 242, 563, 581, 703, 715, 796, 807
Alw21I GWGCWC 1 cut(s) 798
Alw26I GTCTC 3 cut(s) 393, 588, 641
AoxI GGCC 1 cut(s) 49
ApeKI GCWGC 4 cut(s) 120, 205, 758, 761
ApoI RAATTY 1 cut(s) 148
AspS9I GGNCC 3 cut(s) 222, 279, 463
AsuC2I CCSGG 1 cut(s) 63
AsuHPI GGTGA 2 cut(s) 121, 788
AsuNHI GCTAGC 1 cut(s) 602
AvaII GGWCC 3 cut(s) 222, 279, 463
BaeGI GKGCMC 1 cut(s) 79
BaeI ACNNNNGTAYC 2 cut(s) 540, 573
BanI GGYRCC 1 cut(s) 76
BanII GRGCYC 1 cut(s) 798
BauI CACGAG 1 cut(s) 390
Bbv12I GWGCWC 1 cut(s) 798
BbvI GCAGC 4 cut(s) 132, 217, 745, 748
BccI CCATC 2 cut(s) 217, 431
BceAI ACGGC 1 cut(s) 39
BciT130I CCWGG 1 cut(s) 283
BcnI CCSGG 1 cut(s) 63
BcoDI GTCTC 3 cut(s) 393, 588, 641
BfaI CTAG 4 cut(s) 177, 245, 603, 856
BfmI CTRYAG 1 cut(s) 139
BfuAI ACCTGC 1 cut(s) 140
BglII AGATCT 1 cut(s) 695
BisI GCNGC 5 cut(s) 18, 121, 206, 759, 762
BlsI GCNGC 5 cut(s) 19, 122, 207, 760, 763
BmcAI AGTACT 1 cut(s) 663
Bme1390I CCNGG 2 cut(s) 63, 283
Bme18I GGWCC 3 cut(s) 222, 279, 463
BmgT120I GGNCC 3 cut(s) 222, 279, 463
BmiI GGNNCC 4 cut(s) 78, 224, 280, 464
BmrFI CCNGG 2 cut(s) 63, 283
BmtI GCTAGC 1 cut(s) 606
BpuEI CTTGAG 1 cut(s) 148
BpuMI CCSGG 1 cut(s) 63
BsaHI GRCGYC 1 cut(s) 58
Bsc4I CCNNNNNNNGG 2 cut(s) 115, 359
Bse118I RCCGGY 2 cut(s) 108, 828
Bse1I ACTGG 2 cut(s) 330, 845
BseBI CCWGG 1 cut(s) 283
BseGI GGATG 2 cut(s) 150, 631
BseLI CCNNNNNNNGG 2 cut(s) 115, 359
BseNI ACTGG 2 cut(s) 330, 845
BseRI GAGGAG 1 cut(s) 108
BseSI GKGCMC 1 cut(s) 79
BseXI GCAGC 4 cut(s) 132, 217, 745, 748
BshFI GGCC 1 cut(s) 51
BshNI GGYRCC 1 cut(s) 76
BsiHKAI GWGCWC 1 cut(s) 798
BsiSI CCGG 4 cut(s) 52, 63, 109, 829
BslFI GGGAC 4 cut(s) 45, 292, 333, 476
BslI CCNNNNNNNGG 2 cut(s) 115, 359
BsmAI GTCTC 3 cut(s) 393, 588, 641
BsmFI GGGAC 4 cut(s) 45, 292, 333, 476
BsnI GGCC 1 cut(s) 51
Bsp1286I GDGCHC 2 cut(s) 79, 798
Bsp143I GATC 2 cut(s) 167, 695
BspACI CCGC 2 cut(s) 17, 26
BspANI GGCC 1 cut(s) 51
BspLI GGNNCC 4 cut(s) 78, 224, 280, 464
BspMAI CTGCAG 1 cut(s) 143
BspMI ACCTGC 1 cut(s) 140
BspOI GCTAGC 1 cut(s) 606
BspT107I GGYRCC 1 cut(s) 76
BsrFI RCCGGY 2 cut(s) 108, 828
BsrI ACTGG 2 cut(s) 330, 845
BssAI RCCGGY 2 cut(s) 108, 828
BssMI GATC 2 cut(s) 167, 695
BssNI GRCGYC 1 cut(s) 58
BssSI CACGAG 1 cut(s) 390
Bst2BI CACGAG 1 cut(s) 390
Bst2UI CCWGG 1 cut(s) 283
Bst4CI ACNGT 2 cut(s) 69, 818
BstACI GRCGYC 1 cut(s) 58
BstAPI GCANNNNNTGC 1 cut(s) 686
BstC8I GCNNGC 4 cut(s) 38, 106, 156, 604
BstDEI CTNAG 2 cut(s) 521, 699
BstF5I GGATG 2 cut(s) 150, 631
BstKTI GATC 2 cut(s) 170, 698
BstMAI GTCTC 3 cut(s) 393, 588, 641
BstMBI GATC 2 cut(s) 167, 695
BstMWI GCNNNNNNNGC 3 cut(s) 23, 612, 686
BstNI CCWGG 1 cut(s) 283
BstSCI CCNGG 2 cut(s) 61, 281
BstSFI CTRYAG 1 cut(s) 139
BstSLI GKGCMC 1 cut(s) 79
BstV1I GCAGC 4 cut(s) 132, 217, 745, 748
BstX2I RGATCY 1 cut(s) 695
BstYI RGATCY 1 cut(s) 695
BsuRI GGCC 1 cut(s) 51
BtsCI GGATG 2 cut(s) 150, 631
BveI ACCTGC 1 cut(s) 140
Cac8I GCNNGC 4 cut(s) 38, 106, 156, 604
Cfr10I RCCGGY 2 cut(s) 108, 828
Cfr13I GGNCC 3 cut(s) 222, 279, 463
CseI GACGC 2 cut(s) 16, 47
Csp6I GTAC 1 cut(s) 662
CviAII CATG 6 cut(s) 219, 433, 459, 467, 598, 677
CviQI GTAC 1 cut(s) 662
DdeI CTNAG 2 cut(s) 521, 699
DpnI GATC 2 cut(s) 169, 697
DpnII GATC 2 cut(s) 167, 695
DraI TTTAAA 1 cut(s) 822
EciI GGCGGA 1 cut(s) 41
Ecl136II GAGCTC 1 cut(s) 796
Eco24I GRGCYC 1 cut(s) 798
Eco47I GGWCC 3 cut(s) 222, 279, 463
Eco53kI GAGCTC 1 cut(s) 796
EcoICRI GAGCTC 1 cut(s) 796
EcoO109I RGGNCCY 1 cut(s) 279
EcoRII CCWGG 1 cut(s) 281
EcoT38I GRGCYC 1 cut(s) 798
FaeI CATG 6 cut(s) 222, 436, 462, 470, 601, 680
FalI AAGNNNNNCTT 4 cut(s) 626, 658, 648, 680
FaqI GGGAC 4 cut(s) 45, 292, 333, 476
FatI CATG 6 cut(s) 218, 432, 458, 466, 597, 676
Fnu4HI GCNGC 5 cut(s) 18, 121, 206, 759, 762
FokI GGATG 2 cut(s) 157, 638
FriOI GRGCYC 1 cut(s) 798
Fsp4HI GCNGC 5 cut(s) 18, 121, 206, 759, 762
FspBI CTAG 4 cut(s) 177, 245, 603, 856
GluI GCNGC 5 cut(s) 18, 121, 206, 759, 762
HaeIII GGCC 1 cut(s) 51
HapII CCGG 4 cut(s) 52, 63, 109, 829
HgaI GACGC 2 cut(s) 16, 47
Hin1I GRCGYC 1 cut(s) 58
Hin1II CATG 6 cut(s) 222, 436, 462, 470, 601, 680
HincII GTYRAC 3 cut(s) 6, 258, 292
HindII GTYRAC 3 cut(s) 6, 258, 292
HindIII AAGCTT 2 cut(s) 561, 701
HinfI GANTC 3 cut(s) 300, 386, 782
HpaI GTTAAC 1 cut(s) 258
HpaII CCGG 4 cut(s) 52, 63, 109, 829
HphI GGTGA 2 cut(s) 121, 788
Hpy166II GTNNAC 6 cut(s) 6, 114, 258, 292, 502, 631
Hpy188I TCNGA 2 cut(s) 92, 430
Hpy188III TCNNGA 1 cut(s) 165
Hpy8I GTNNAC 6 cut(s) 6, 114, 258, 292, 502, 631
Hpy99I CGWCG 1 cut(s) 25
HpyAV CCTTC 2 cut(s) 280, 722
HpyCH4III ACNGT 2 cut(s) 69, 818
HpyCH4V TGCA 6 cut(s) 40, 135, 141, 154, 680, 689
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 612, 686
HpyF3I CTNAG 2 cut(s) 521, 699
Hsp92I GRCGYC 1 cut(s) 58
Hsp92II CATG 6 cut(s) 222, 436, 462, 470, 601, 680
KspAI GTTAAC 1 cut(s) 258
Kzo9I GATC 2 cut(s) 167, 695
Lsp1109I GCAGC 4 cut(s) 132, 217, 745, 748
MaeI CTAG 4 cut(s) 177, 245, 603, 856
MalI GATC 2 cut(s) 169, 697
MboI GATC 2 cut(s) 167, 695
MboII GAAGA 1 cut(s) 245
MflI RGATCY 1 cut(s) 695
MhlI GDGCHC 2 cut(s) 79, 798
MluCI AATT 5 cut(s) 148, 355, 723, 768, 789
MlyI GAGTC 2 cut(s) 395, 776
MmeI TCCRAC 1 cut(s) 70
MnlI CCTC 8 cut(s) 37, 41, 48, 79, 86, 137, 244, 748
MseI TTAA 6 cut(s) 257, 365, 396, 528, 821, 849
MslI CAYNNNNRTG 1 cut(s) 217
MspI CCGG 4 cut(s) 52, 63, 109, 829
MspR9I CCNGG 2 cut(s) 63, 283
MvaI CCWGG 1 cut(s) 283
MwoI GCNNNNNNNGC 3 cut(s) 23, 612, 686
NciI CCSGG 1 cut(s) 63
NdeII GATC 2 cut(s) 167, 695
NheI GCTAGC 1 cut(s) 602
NlaIII CATG 6 cut(s) 222, 436, 462, 470, 601, 680
NlaIV GGNNCC 4 cut(s) 78, 224, 280, 464
PaqCI CACCTGC 1 cut(s) 140
PfeI GAWTC 1 cut(s) 300
PflMI CCANNNNNTGG 1 cut(s) 359
PfoI TCCNGGA 1 cut(s) 61
PkrI GCNGC 5 cut(s) 19, 122, 207, 760, 763
PleI GAGTC 2 cut(s) 394, 776
PpsI GAGTC 2 cut(s) 394, 776
PpuMI RGGWCCY 1 cut(s) 279
PsiI TTATAA 1 cut(s) 719
Psp124BI GAGCTC 1 cut(s) 798
Psp5II RGGWCCY 1 cut(s) 279
Psp6I CCWGG 1 cut(s) 281
PspGI CCWGG 1 cut(s) 281
PspN4I GGNNCC 4 cut(s) 78, 224, 280, 464
PspPI GGNCC 3 cut(s) 222, 279, 463
PspPPI RGGWCCY 1 cut(s) 279
PstI CTGCAG 1 cut(s) 143
PsuI RGATCY 1 cut(s) 695
RsaI GTAC 1 cut(s) 663
RsaNI GTAC 1 cut(s) 662
RseI CAYNNNNRTG 1 cut(s) 217
SacI GAGCTC 1 cut(s) 798
SaqAI TTAA 6 cut(s) 257, 365, 396, 528, 821, 849
SatI GCNGC 5 cut(s) 18, 121, 206, 759, 762
Sau3AI GATC 2 cut(s) 167, 695
Sau96I GGNCC 3 cut(s) 222, 279, 463
ScaI AGTACT 1 cut(s) 663
SchI GAGTC 2 cut(s) 395, 776
ScrFI CCNGG 2 cut(s) 63, 283
SduI GDGCHC 2 cut(s) 79, 798
SfcI CTRYAG 1 cut(s) 139
SinI GGWCC 3 cut(s) 222, 279, 463
SmiMI CAYNNNNRTG 1 cut(s) 217
SmlI CTYRAG 1 cut(s) 163
SmoI CTYRAG 1 cut(s) 163
Sse9I AATT 5 cut(s) 148, 355, 723, 768, 789
SsiI CCGC 2 cut(s) 17, 26
SspI AATATT 1 cut(s) 334
SspMI CTAG 4 cut(s) 177, 245, 603, 856
SstI GAGCTC 1 cut(s) 798
StyD4I CCNGG 2 cut(s) 61, 281
TaaI ACNGT 2 cut(s) 69, 818
TaqI TCGA 1 cut(s) 84
TasI AATT 5 cut(s) 148, 355, 723, 768, 789
TatI WGTACW 1 cut(s) 661
TauI GCSGC 1 cut(s) 20
TfiI GAWTC 1 cut(s) 300
Tru1I TTAA 6 cut(s) 257, 365, 396, 528, 821, 849
Tru9I TTAA 6 cut(s) 257, 365, 396, 528, 821, 849
TseI GCWGC 4 cut(s) 120, 205, 758, 761
TspDTI ATGAA 4 cut(s) 161, 483, 526, 530
Van91I CCANNNNNTGG 1 cut(s) 359
VpaK11BI GGWCC 3 cut(s) 222, 279, 463
XapI RAATTY 1 cut(s) 148
XcmI CCANNNNNNNNNTGG 1 cut(s) 216
XspI CTAG 4 cut(s) 177, 245, 603, 856
ZrmI AGTACT 1 cut(s) 663
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.