Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0000916

Physical Location & Seq

Genomic Coordinates Reverse (-)

29242371 .. 29244806

Gene Structure 2436 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1233200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2436 bp

ATGGATATGATGAATAGCGGCAGAAACAAAATCAGTTACAAGTCATTGGTTGTGGTTCTTGTGTTCTGCTGTACCTTGAAAGCCGCCGATATCTCGGCGGCGTCAAGCAACATGATCTCCCAAGGACAATCTATTTCCGGCAACCATACAATCACCTCATCACCACGCGGCATATTCGAGCTAGGTTTCTTCACTCCAGGTAACTCCAACAACTACTACATAGGCATCTGGTACAAAAATGTACAGGAAACTGTCGTTTGGGTGGCCAACAGAAACCACCCTGTTTCCGATCCTTTTTTTTATCATCCCTTCAAACTCTTCCCAAACGGAACCTTAGCACTTTTCGACCAGTCCAAATCTGCCATTTGGTCAACAACTCGTGATCTCTCAATTGCTTCCAATTCTACTAGTCAAGTAGCAGCAATACTTCTTGACAGCGGCAACTTTGTCATAACAGATGCTTTGAATTCGTCTGCTGTCATATGGCAGAGTTTCGATCACCCGACGGACACTTGGCTGCCGGGCGGGAAGCTTGGATACAACAAGCTGACCAATGAGAAACTAACCCTCACTCCCTGGAGAAGCTCACAAAACCCAGCACCTGGGATTTTCTCTCTAGACCTCGAACAAAACGGGACGAGTTTGTTGCTGATGTATAATGGGACTAGAATGTATTGGACAACTGGTCCTTGGACAGGCAAGATTTTTACGAAAGTTCCTGAAATTCAGTTGAATTATTTGATCACGAATATCAGCTATGTTTCCAATGAGCTGGGGAGTTATTTTTCTTATGATGCAGTTTACCCCGACATCTTTGTTAAGTACATGCTTGATATCTCCGGGCAGTTAAGGGCCTCCAAGTGGGGGAGGGACTTCCATCAATGGACTTCATTTTGGCTGCGACCCTCCGAACAATGTGACGTCTATGGATTCTGCGGTGCTTCTAGCATTTGCAATCAGCAACAAGTGCCTCTTTGTGTTTGCTTGGAAGGGTTTGAACCTCGATCCCCAAAAGATTGGGACTCGGCGGATTCCTGGGAAGGGTGTGTGAGGAAACCCCCTTTAGAATGCAGTAGTGGAGGAAATGAAACGTTTGTGGTGATACCCGATTTAAGCTTTCCAGAGAATTCGGAGACTTTAGCAGGTAAGAACATCGATGAATGTAGATTGGCATGCTTGAGTGATTGCTCGTGTACAGCTTTTGCTTATGACAGTAGGTGTTTGGTTTGGAAAGGAGATCTGTTCAATGTAAAACAACTTACATCTGATGAGAACGTAGGCAAAGAGTGGCATCTTCGAGTTCCAAAGGCGAAGAAAGAGAATAAAACCCTTTGGATTGTCACTGGAGTACTTGGAGGTCTGCTTGGTATTTTACTAGTTATTGTGGTAATTGCCAAAAATAAATGTTCCGGTTCTGGGGAAAGGTCTGAGGCAGATGATGGCTCTTTGATGATGTTCAAGTACAGGATTCTAAGAAAAGCAACCAAGAACTTTACTGAAAAACTTGGGGAAGGAGGCTTTGGTTCTGTTTTCAGAGGGACGCTGAAAAATTCTACTGCCATCGCAGTGAAGAGACTTAACTGTCCAGAGCAAGCCGATAATCAATTTCTTACAGAAGTGAGGACTCTTGGAAAAGTCCAACATATCAATCTTGTTCGTCTCCGCGGATTTTGTGCAGAAACTTCAAAAAGATTGTTGGTTTATGATTACATGCCAAATGGTTCTCTAGAATCGCTTTTGTTTCAAAAGAGTCCCATCGTCTTGGATTGGAAAACTAGATATCACATTGCAGTTGGCACTGCGAAAGGACTTGCATACCTTCATGATAGTTGCAGAGAATGCATCATACACTGCGACATCAAACCCGAAAACATTTTGTTGGATGCAGAATATGCCCCCAAGATAACAGATTTCGGCCTAGCAAAGCTCATGAGCAGAGACTTCAGCCGGATAATTACAACCATACAAGGAACAAGGGGGTATATTGCTCCGGAATGGATATCAGGAGAAGCTATCACAGTAAAAGCCGATGTCTTTAGTTATGGAATGTTGTTTTTCGAGATCATATCAGGAAGGAGAAACCGAGATCTTTTGGATGATGGGTTGGAAAACTACTTCCCAACTCACGTTGCAAATGTATTAACGAAAGGAGAAAATGTCGATACCCTGTTGGACAGCAGGTTAGAAGGCAATGCTAACAAAGAAGAATTGATGAGAGCATGCAAAGTAGCTTGTTGGTGCATTCAAGATGACGAGAAGGATAGGCCAACAATGGGGCAGGTTGTTCAAGCTTTGGAAGGAGTTATAGATCTCGGCATGCCTCCCGTCCCCCACTTCATGGACCGCTTTTCGAAGAGTCATTTTGAGTCCATACATTACCATGACATTTCTTCCAGCACTGCTTCGGATTCAAGGCGTGGCTACTCAAGTAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

B_lectin PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

812

Amino Acids

90.71

Weight (kDa)

6.35

Isoelectric Point (pI)

41.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	84 - 194	3.7e-31	D-mannose binding lectin
S_locus_glycop	PF00954	226 - 335	5.1e-25	S-locus glycoprotein domain
PAN_2	PF08276	357 - 417	3.8e-14	PAN-like domain
PK_Tyr_Ser-Thr	PF07714	498 - 765	3.6e-43	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	499 - 763	1.8e-49	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0000916)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g26420 FvH4_6g26420 FvH4_6g26450 FvH4_6g26451
malus_domestica	MD09G1116700.v1.1 MD09G1233200.v1.1 MD12G1033500.v1.1 MD14G1034400.v1.1 MD14G1034600.v1.1 MD14G1034700.v1.1 MD14G1034800.v1.1 MD14G1034900.v1.1 MD14G1035200.v1.1 MD17G1090300.v1.1 MD17G1108200.v1.1 MD17G1237700.v1.1 MD17G1237800.v1.1 MD17G1238000.v1.1 MD17G1238400.v1.1
prunus_persica	Prupe.3G131100_v2.0.a1 Prupe.3G141400_v2.0.a1
pyrus_communis	pycom09g04120 pycom12g03080 pycom14g03020 pycom14g03080 pycom14g03090 pycom17g24250
rosa_chinensis	RchiOBHm_Chr2g0127791 RchiOBHm_Chr2g0127811 RchiOBHm_Chr2g0127821 RchiOBHm_Chr2g0128001
rosa_laevigata	RLG00000002113 RLG00000018931 RLG00000018932 RLG00000018933 RLG00000018934 RLG00000023426
rosa_multiflora	Rmu_sc0000516.1_g000026 Rmu_sc0000516.1_g000045 Rmu_ssc0000093.1_g000010 Rmu_ssc0000093.1_g000016 Rmu_ssc0000093.1_g000017 Rmu_ssc0000093.1_g000024
rosa_roxburghii	Rroxscaffold_2G00116790 Rroxscaffold_2G00116800 Rroxscaffold_2G00116810 Rroxscaffold_2G00116840
rosa_rugosa	Rorug02G0270500 Rorug02G0270600 Rorug02G0270800 Rorug02G0270900 Rorug02G0271000
rosa_samantha	Rh2AG323100 Rh2BG332100 Rh2BG332200 Rh2BG332300 Rh2CG310900 Rh2CG311000 Rh2DG350400 Rh2DG350500 Rh2DG350600 Rh2DG350700
rosa_wichuraiana	Rw2G026190 Rw2G026210 Rw2G026230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1581
AatII	GACGTC	1 cut(s)	924
Acc36I	ACCTGC	3 cut(s)	1133, 2169, 2269
AccB7I	CCANNNNNTGG	2 cut(s)	602, 2338
AccII	CGCG	2 cut(s)	168, 1665
AccIII	TCCGGA	1 cut(s)	1990
AclI	AACGTT	1 cut(s)	1091
AclWI	GGATC	2 cut(s)	284, 999
AcoI	YGGCCR	1 cut(s)	264
AcsI	RAATTY	4 cut(s)	466, 723, 1126, 1549
AcuI	CTGAAG	1 cut(s)	1927
AcyI	GRCGYC	2 cut(s)	101, 921
AfaI	GTAC	7 cut(s)	73, 233, 243, 824, 1195, 1350, 1463
AfiI	CCNNNNNNNGG	8 cut(s)	602, 695, 861, 864, 1041, 1416, 2273, 2338
AhdI	GACNNNNNGTC	1 cut(s)	684
AhlI	ACTAGT	2 cut(s)	407, 1375
AjnI	CCWGG	4 cut(s)	196, 575, 601, 1034
AjuI	GAANNNNNNNTTGG	2 cut(s)	1503, 1535
Alw26I	GTCTC	4 cut(s)	1127, 1567, 1664, 1932
AlwI	GGATC	2 cut(s)	284, 999
AlwNI	CAGNNNCTG	1 cut(s)	602
Aor13HI	TCCGGA	1 cut(s)	1990
AoxI	GGCC	4 cut(s)	264, 852, 1915, 2264
ApeKI	GCWGC	3 cut(s)	419, 517, 898
ApoI	RAATTY	4 cut(s)	466, 723, 1126, 1549
AspS9I	GGNCC	3 cut(s)	686, 852, 2341
AsuC2I	CCSGG	2 cut(s)	522, 841
AsuHPI	GGTGA	4 cut(s)	145, 153, 491, 1111
AsuII	TTCGAA	1 cut(s)	2351
AvaII	GGWCC	2 cut(s)	686, 2341
BalI	TGGCCA	1 cut(s)	266
BarI	GAAGNNNNNNTAC	2 cut(s)	521, 553
BauI	CACGAG	2 cut(s)	378, 1189
BbvI	GCAGC	3 cut(s)	431, 504, 885
BccI	CCATC	5 cut(s)	885, 1433, 1568, 1763, 2093
BcgI	CGANNNNNNTGC	2 cut(s)	628, 662
BciT130I	CCWGG	4 cut(s)	198, 577, 603, 1036
BciVI	GTATCC	1 cut(s)	530
BclI	TGATCA	1 cut(s)	741
BcnI	CCSGG	2 cut(s)	522, 841
BcoDI	GTCTC	4 cut(s)	1127, 1567, 1664, 1932
BcuI	ACTAGT	2 cut(s)	407, 1375
BfaI	CTAG	9 cut(s)	182, 408, 617, 666, 945, 1376, 1727, 1776, 1919
BfuAI	ACCTGC	3 cut(s)	1133, 2169, 2269
BfuI	GTATCC	1 cut(s)	530
BglII	AGATCT	3 cut(s)	1237, 2086, 2308
BisI	GCNGC	8 cut(s)	19, 84, 99, 169, 420, 439, 518, 899
BlsI	GCNGC	8 cut(s)	20, 85, 100, 170, 421, 440, 519, 900
BmcAI	AGTACT	1 cut(s)	1350
Bme1390I	CCNGG	6 cut(s)	198, 522, 577, 603, 841, 1036
Bme18I	GGWCC	2 cut(s)	686, 2341
BmeRI	GACNNNNNGTC	1 cut(s)	684
BmgT120I	GGNCC	3 cut(s)	686, 852, 2341
BmiI	GGNNCC	1 cut(s)	331
BmrFI	CCNGG	6 cut(s)	198, 522, 577, 603, 841, 1036
BmsI	GCATC	6 cut(s)	234, 448, 784, 1300, 1851, 1873
BpmI	CTGGAG	3 cut(s)	180, 598, 1365
Bpu10I	CCTNAGC	1 cut(s)	334
Bpu14I	TTCGAA	1 cut(s)	2351
BpuEI	CTTGAG	2 cut(s)	1198, 2410
BpuMI	CCSGG	2 cut(s)	522, 841
Bsa29I	ATCGAT	1 cut(s)	1155
BsaHI	GRCGYC	2 cut(s)	101, 921
BsaJI	CCNNGG	6 cut(s)	121, 575, 602, 689, 1035, 1663
BsaWI	WCCGGW	2 cut(s)	1409, 1990
BsaXI	ACNNNNNCTCC	6 cut(s)	101, 131, 556, 586, 2142, 2172
Bsc4I	CCNNNNNNNGG	8 cut(s)	602, 695, 861, 864, 1041, 1416, 2273, 2338
Bse1I	ACTGG	3 cut(s)	349, 688, 1348
Bse3DI	GCAATG	2 cut(s)	1785, 2197
BseAI	TCCGGA	1 cut(s)	1990
BseBI	CCWGG	4 cut(s)	198, 577, 603, 1036
BseCI	ATCGAT	1 cut(s)	1155
BseDI	CCNNGG	6 cut(s)	121, 575, 602, 689, 1035, 1663
BseGI	GGATG	3 cut(s)	304, 1888, 2101
BseLI	CCNNNNNNNGG	8 cut(s)	602, 695, 861, 864, 1041, 1416, 2273, 2338
BseMI	GCAATG	2 cut(s)	1785, 2197
BseMII	CTCAG	1 cut(s)	1419
BseNI	ACTGG	3 cut(s)	349, 688, 1348
BseXI	GCAGC	3 cut(s)	431, 504, 885
BseYI	CCCAGC	2 cut(s)	595, 772
BsgI	GTGCAG	1 cut(s)	1695
Bsh1236I	CGCG	2 cut(s)	168, 1665
BshFI	GGCC	4 cut(s)	266, 854, 1917, 2266
BshVI	ATCGAT	1 cut(s)	1155
BsiSI	CCGG	6 cut(s)	138, 521, 840, 1410, 1948, 1991
BslFI	GGGAC	7 cut(s)	649, 676, 884, 1034, 1552, 1737, 2312
BslI	CCNNNNNNNGG	8 cut(s)	602, 695, 861, 864, 1041, 1416, 2273, 2338
BsmAI	GTCTC	4 cut(s)	1127, 1567, 1664, 1932
BsmBI	CGTCTC	1 cut(s)	1664
BsmFI	GGGAC	7 cut(s)	649, 676, 884, 1034, 1552, 1737, 2312
BsmI	GAATGC	3 cut(s)	1073, 1844, 2241
BsnI	GGCC	4 cut(s)	266, 854, 1917, 2266
Bsp119I	TTCGAA	1 cut(s)	2351
Bsp13I	TCCGGA	1 cut(s)	1990
Bsp1407I	TGTACA	2 cut(s)	241, 1193
BspANI	GGCC	4 cut(s)	266, 854, 1917, 2266
BspCNI	CTCAG	1 cut(s)	1420
BspDI	ATCGAT	1 cut(s)	1155
BspEI	TCCGGA	1 cut(s)	1990
BspFNI	CGCG	2 cut(s)	168, 1665
BspHI	TCATGA	2 cut(s)	1822, 1929
BspLI	GGNNCC	1 cut(s)	331
BspMI	ACCTGC	3 cut(s)	1133, 2169, 2269
BspPI	GGATC	2 cut(s)	284, 999
BspT104I	TTCGAA	1 cut(s)	2351
BsrDI	GCAATG	2 cut(s)	1785, 2197
BsrGI	TGTACA	2 cut(s)	241, 1193
BsrI	ACTGG	3 cut(s)	349, 688, 1348
BssECI	CCNNGG	6 cut(s)	121, 575, 602, 689, 1035, 1663
BssNI	GRCGYC	2 cut(s)	101, 921
BssSI	CACGAG	2 cut(s)	378, 1189
BssT1I	CCWWGG	2 cut(s)	121, 689
Bst2BI	CACGAG	2 cut(s)	378, 1189
Bst2UI	CCWGG	4 cut(s)	198, 577, 603, 1036
Bst4CI	ACNGT	4 cut(s)	253, 1214, 1583, 2020
Bst6I	CTCTTC	3 cut(s)	323, 1565, 2348
BstACI	GRCGYC	2 cut(s)	101, 921
BstAPI	GCANNNNNTGC	3 cut(s)	967, 1839, 1892
BstAUI	TGTACA	2 cut(s)	241, 1193
BstBI	TTCGAA	1 cut(s)	2351
BstC8I	GCNNGC	4 cut(s)	1174, 1593, 2221, 2318
BstDEI	CTNAG	3 cut(s)	334, 1428, 1472
BstDSI	CCRYGG	1 cut(s)	1663
BstENI	CCTNNNNNAGG	1 cut(s)	693
BstF5I	GGATG	3 cut(s)	304, 1888, 2101
BstFNI	CGCG	2 cut(s)	168, 1665
BstMAI	GTCTC	4 cut(s)	1127, 1567, 1664, 1932
BstMWI	GCNNNNNNNGC	3 cut(s)	967, 1839, 1892
BstNI	CCWGG	4 cut(s)	198, 577, 603, 1036
BstNSI	RCATGY	5 cut(s)	829, 1176, 1714, 2223, 2320
BstSCI	CCNGG	6 cut(s)	196, 520, 575, 601, 839, 1034
BstUI	CGCG	2 cut(s)	168, 1665
BstV1I	GCAGC	3 cut(s)	431, 504, 885
BstX2I	RGATCY	3 cut(s)	1237, 2086, 2308
BstXI	CCANNNNNNTGG	3 cut(s)	772, 1017, 1762
BstYI	RGATCY	3 cut(s)	1237, 2086, 2308
Bsu15I	ATCGAT	1 cut(s)	1155
BsuI	GTATCC	1 cut(s)	530
BsuRI	GGCC	4 cut(s)	266, 854, 1917, 2266
BsuTUI	ATCGAT	1 cut(s)	1155
BtgI	CCRYGG	1 cut(s)	1663
BtgZI	GCGATG	1 cut(s)	1546
BtsCI	GGATG	3 cut(s)	304, 1888, 2101
BtsI	GCAGTG	4 cut(s)	1572, 1797, 1849, 2397
BtsIMutI	CAGTG	5 cut(s)	1341, 1572, 1797, 1849, 2397
BveI	ACCTGC	3 cut(s)	1133, 2169, 2269
Cac8I	GCNNGC	4 cut(s)	1174, 1593, 2221, 2318
CaiI	CAGNNNCTG	1 cut(s)	602
CciI	TCATGA	2 cut(s)	1822, 1929
Cfr13I	GGNCC	3 cut(s)	686, 852, 2341
Cfr42I	CCGCGG	1 cut(s)	1666
ClaI	ATCGAT	1 cut(s)	1155
CseI	GACGC	2 cut(s)	90, 1549
Csp6I	GTAC	7 cut(s)	72, 232, 242, 823, 1194, 1349, 1462
CviQI	GTAC	7 cut(s)	72, 232, 242, 823, 1194, 1349, 1462
DdeI	CTNAG	3 cut(s)	334, 1428, 1472
DrdI	GACNNNNNNGTC	1 cut(s)	1581
DriI	GACNNNNNGTC	1 cut(s)	684
DseDI	GACNNNNNNGTC	1 cut(s)	1581
EaeI	YGGCCR	1 cut(s)	264
Eam1104I	CTCTTC	3 cut(s)	323, 1565, 2348
Eam1105I	GACNNNNNGTC	1 cut(s)	684
EarI	CTCTTC	3 cut(s)	323, 1565, 2348
EciI	GGCGGA	1 cut(s)	1043
Eco130I	CCWWGG	2 cut(s)	121, 689
Eco32I	GATATC	4 cut(s)	91, 835, 1781, 2001
Eco47I	GGWCC	2 cut(s)	686, 2341
Eco57I	CTGAAG	1 cut(s)	1927
EcoNI	CCTNNNNNAGG	1 cut(s)	693
EcoO109I	RGGNCCY	1 cut(s)	852
EcoRI	GAATTC	2 cut(s)	466, 1126
EcoRII	CCWGG	4 cut(s)	196, 575, 601, 1034
EcoRV	GATATC	4 cut(s)	91, 835, 1781, 2001
EcoT14I	CCWWGG	2 cut(s)	121, 689
EcoT22I	ATGCAT	1 cut(s)	1844
ErhI	CCWWGG	2 cut(s)	121, 689
Esp3I	CGTCTC	1 cut(s)	1664
FalI	AAGNNNNNCTT	2 cut(s)	957, 989
FaqI	GGGAC	7 cut(s)	649, 676, 884, 1034, 1552, 1737, 2312
FauI	CCCGC	1 cut(s)	518
FauNDI	CATATG	1 cut(s)	482
FbaI	TGATCA	1 cut(s)	741
Fnu4HI	GCNGC	8 cut(s)	19, 84, 99, 169, 420, 439, 518, 899
FokI	GGATG	3 cut(s)	291, 1895, 2108
Fsp4HI	GCNGC	8 cut(s)	19, 84, 99, 169, 420, 439, 518, 899
FspBI	CTAG	9 cut(s)	182, 408, 617, 666, 945, 1376, 1727, 1776, 1919
GluI	GCNGC	8 cut(s)	19, 84, 99, 169, 420, 439, 518, 899
GsaI	CCCAGC	2 cut(s)	599, 776
GsuI	CTGGAG	3 cut(s)	180, 598, 1365
HaeIII	GGCC	4 cut(s)	266, 854, 1917, 2266
HapII	CCGG	6 cut(s)	138, 521, 840, 1410, 1948, 1991
HgaI	GACGC	2 cut(s)	90, 1549
Hin1I	GRCGYC	2 cut(s)	101, 921
HincII	GTYRAC	1 cut(s)	372
HindII	GTYRAC	1 cut(s)	372
HindIII	AAGCTT	3 cut(s)	530, 1114, 2289
HpaII	CCGG	6 cut(s)	138, 521, 840, 1410, 1948, 1991
HphI	GGTGA	4 cut(s)	145, 153, 491, 1111
Hpy166II	GTNNAC	3 cut(s)	372, 802, 1194
Hpy188I	TCNGA	7 cut(s)	289, 910, 1132, 1267, 1429, 1535, 2407
Hpy8I	GTNNAC	3 cut(s)	372, 802, 1194
Hpy99I	CGWCG	1 cut(s)	508
HpyAV	CCTTC	9 cut(s)	319, 983, 1034, 1505, 1829, 2067, 2180, 2251, 2291
HpyCH4III	ACNGT	4 cut(s)	253, 1214, 1583, 2020
HpyCH4IV	ACGT	4 cut(s)	921, 1091, 1275, 2127
HpyF10VI	GCNNNNNNNGC	3 cut(s)	967, 1839, 1892
HpyF3I	CTNAG	3 cut(s)	334, 1428, 1472
HpySE526I	ACGT	4 cut(s)	921, 1091, 1275, 2127
Hsp92I	GRCGYC	2 cut(s)	101, 921
Kpn2I	TCCGGA	1 cut(s)	1990
Ksp22I	TGATCA	1 cut(s)	741
KspI	CCGCGG	1 cut(s)	1666
LmnI	GCTCC	1 cut(s)	1993
Lsp1109I	GCAGC	3 cut(s)	431, 504, 885
LweI	GCATC	6 cut(s)	234, 448, 784, 1300, 1851, 1873
MaeI	CTAG	9 cut(s)	182, 408, 617, 666, 945, 1376, 1727, 1776, 1919
MaeII	ACGT	4 cut(s)	921, 1091, 1275, 2127
MaeIII	GTNAC	4 cut(s)	35, 200, 917, 1339
MboII	GAAGA	8 cut(s)	181, 310, 1286, 1324, 1582, 2216, 2365, 2382
MfeI	CAATTG	1 cut(s)	390
MflI	RGATCY	3 cut(s)	1237, 2086, 2308
MlsI	TGGCCA	1 cut(s)	266
MluNI	TGGCCA	1 cut(s)	266
MlyI	GAGTC	5 cut(s)	1016, 1618, 1759, 2365, 2375
MmeI	TCCRAC	5 cut(s)	231, 1663, 1860, 2085, 2151
Mox20I	TGGCCA	1 cut(s)	266
Mph1103I	ATGCAT	1 cut(s)	1844
MroI	TCCGGA	1 cut(s)	1990
MscI	TGGCCA	1 cut(s)	266
MseI	TTAA	5 cut(s)	819, 848, 1112, 1578, 2141
MslI	CAYNNNNRTG	2 cut(s)	1565, 2379
Msp20I	TGGCCA	1 cut(s)	266
MspA1I	CMGCKG	2 cut(s)	438, 1665
MspI	CCGG	6 cut(s)	138, 521, 840, 1410, 1948, 1991
MspR9I	CCNGG	6 cut(s)	198, 522, 577, 603, 841, 1036
MunI	CAATTG	1 cut(s)	390
Mva1269I	GAATGC	3 cut(s)	1073, 1844, 2241
MvaI	CCWGG	4 cut(s)	198, 577, 603, 1036
MvnI	CGCG	2 cut(s)	168, 1665
MwoI	GCNNNNNNNGC	3 cut(s)	967, 1839, 1892
NciI	CCSGG	2 cut(s)	522, 841
NdeI	CATATG	1 cut(s)	482
NlaIV	GGNNCC	1 cut(s)	331
NmeAIII	GCCGAG	3 cut(s)	74, 1004, 2292
NmuCI	GTSAC	2 cut(s)	917, 1339
NsiI	ATGCAT	1 cut(s)	1844
NspI	RCATGY	5 cut(s)	829, 1176, 1714, 2223, 2320
NspV	TTCGAA	1 cut(s)	2351
PaeI	GCATGC	3 cut(s)	1176, 2223, 2320
PagI	TCATGA	2 cut(s)	1822, 1929
PctI	GAATGC	3 cut(s)	1073, 1844, 2241
PfeI	GAWTC	5 cut(s)	930, 1031, 1468, 1730, 2408
PflMI	CCANNNNNTGG	2 cut(s)	602, 2338
PkrI	GCNGC	8 cut(s)	20, 85, 100, 170, 421, 440, 519, 900
PleI	GAGTC	5 cut(s)	1016, 1618, 1758, 2364, 2374
PpsI	GAGTC	5 cut(s)	1016, 1618, 1758, 2364, 2374
Psp1406I	AACGTT	1 cut(s)	1091
Psp6I	CCWGG	4 cut(s)	196, 575, 601, 1034
PspFI	CCCAGC	2 cut(s)	595, 772
PspGI	CCWGG	4 cut(s)	196, 575, 601, 1034
PspN4I	GGNNCC	1 cut(s)	331
PspPI	GGNCC	3 cut(s)	686, 852, 2341
PstNI	CAGNNNCTG	1 cut(s)	602
PsuI	RGATCY	3 cut(s)	1237, 2086, 2308
RsaI	GTAC	7 cut(s)	73, 233, 243, 824, 1195, 1350, 1463
RsaNI	GTAC	7 cut(s)	72, 232, 242, 823, 1194, 1349, 1462
RseI	CAYNNNNRTG	2 cut(s)	1565, 2379
SacII	CCGCGG	1 cut(s)	1666
SaqAI	TTAA	5 cut(s)	819, 848, 1112, 1578, 2141
SatI	GCNGC	8 cut(s)	19, 84, 99, 169, 420, 439, 518, 899
Sau96I	GGNCC	3 cut(s)	686, 852, 2341
ScaI	AGTACT	1 cut(s)	1350
SchI	GAGTC	5 cut(s)	1016, 1618, 1759, 2365, 2375
ScrFI	CCNGG	6 cut(s)	198, 522, 577, 603, 841, 1036
SfaNI	GCATC	6 cut(s)	234, 448, 784, 1300, 1851, 1873
Sfr303I	CCGCGG	1 cut(s)	1666
SfuI	TTCGAA	1 cut(s)	2351
SgrBI	CCGCGG	1 cut(s)	1666
SinI	GGWCC	2 cut(s)	686, 2341
SmiMI	CAYNNNNRTG	2 cut(s)	1565, 2379
SmlI	CTYRAG	2 cut(s)	1177, 2425
SmoI	CTYRAG	2 cut(s)	1177, 2425
SpeI	ACTAGT	2 cut(s)	407, 1375
SphI	GCATGC	3 cut(s)	1176, 2223, 2320
SspMI	CTAG	9 cut(s)	182, 408, 617, 666, 945, 1376, 1727, 1776, 1919
StyD4I	CCNGG	6 cut(s)	196, 520, 575, 601, 839, 1034
StyI	CCWWGG	2 cut(s)	121, 689
TaaI	ACNGT	4 cut(s)	253, 1214, 1583, 2020
TaiI	ACGT	4 cut(s)	924, 1094, 1278, 2130
TatI	WGTACW	5 cut(s)	241, 822, 1193, 1348, 1461
TauI	GCSGC	5 cut(s)	21, 86, 101, 171, 441
TfiI	GAWTC	5 cut(s)	930, 1031, 1468, 1730, 2408
Tru1I	TTAA	5 cut(s)	819, 848, 1112, 1578, 2141
Tru9I	TTAA	5 cut(s)	819, 848, 1112, 1578, 2141
TscAI	CASTG	5 cut(s)	1348, 1572, 1804, 1856, 2404
TseFI	GTSAC	2 cut(s)	917, 1339
TseI	GCWGC	3 cut(s)	419, 517, 898
Tsp45I	GTSAC	2 cut(s)	917, 1339
TspDTI	ATGAA	6 cut(s)	26, 879, 1101, 1173, 1811, 2326
TspGWI	ACGGA	2 cut(s)	342, 521
TspRI	CASTG	5 cut(s)	1348, 1572, 1804, 1856, 2404
Van91I	CCANNNNNTGG	2 cut(s)	602, 2338
VpaK11BI	GGWCC	2 cut(s)	686, 2341
XagI	CCTNNNNNAGG	1 cut(s)	693
XapI	RAATTY	4 cut(s)	466, 723, 1126, 1549
XbaI	TCTAGA	2 cut(s)	616, 1726
XceI	RCATGY	5 cut(s)	829, 1176, 1714, 2223, 2320
XspI	CTAG	9 cut(s)	182, 408, 617, 666, 945, 1376, 1727, 1776, 1919
ZraI	GACGTC	1 cut(s)	922
ZrmI	AGTACT	1 cut(s)	1350
Zsp2I	ATGCAT	1 cut(s)	1844

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.