Rroxscaffold_2G00116800

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000916
Physical Location & Seq
Forward (+)
44046485 .. 44048901
2417 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00116800.1

Sequence Viewer

Length: 2361 bp
ATGAAAACTAGTATCAGAAACCACTTGATTTCCCAGTCTCTGCTACTCTTGTTATCTTACACTACTTGTACTCTCAAAGCTATTGAGTTTCTGTTACCAGGCCAATCACTCAATGGGGACCAGAGGATCACCTCTCCAGGTGGCATTTTTGAACTTGGTTTCTTCACACCAGGTAACTCTCAAAACCATTACATAGGCATCTGGTACAAAAATCTACCCAATCAAACAGTTGTTTGGGTGGCAAACAGAAACCAACCTGTTGCTGATCCCATTTCTTCAACATTTCTACTCCTTGAAAATGGAAACTTGACCTTAAACAGCCCCTCAACAGCGGCAATATGGTCAACAAATTCAACGTCACAGGTTAGAAATTCTACTATAGCCATGCTTCTTGACGATGGCAACTTTGTCATAAGAGATGCATCTGCAGTACTATGGCAGAGTTTCGATCATCCAACCAATACTTGGCTGCCAGGTGCAAGGCTTGGATATAACAAGCTTACCAAGCAAATCCTAAGTATTACCCCCTGGAGAAATCCGCAAAATCCAGCACCTGGTCCCTTTTCTTTTGAGCTAGAACAAACAAGTCTTCGGCTATTATGGAATAGTTCAGAAACTTATTGGACCAGCGGTAATTGGACAGGGAAAACTTTCAGCCATCTTCAAGACATAGAAGAGAAGCATGTTCCCTCAATTTTCTCATTTGTTTTAAATGAGACTGGAAGCTACCTTAGTTATGCAGTTCGTGCTTCTGCCTATCCTGATCCCTTTATTAGGAATCATACCTTTATTAGGTACATGATAGAAACCACTGGTCAAATGAAGTTCTTTGTCATGGAGAACGGCTCCCTGCAATGGAATTTGCTATGGATGGATCCAATGGAACGATGTGATGTTTATGCTTCCTGTGGTGCTTTTAGTATATGCAACCCACGTAATCTTCCTCGGTGTGGCTGTCTAAAAGGATTCGAACCCAAATTCCATAAAGACTGGGAATTAGAAGATCACTCAAATGGCTGTGGGAGGAAAACTTCTTTTCATTGCAGTGTTGAGGGGAATTCTGCATTCCTGGTGATGGAGAATGTTACTTACCCGAAGAGTCCTGACTCCTTGGTAGTTGATAACATTGACAAATTGTCTGATTTGGGGGGTGATCTGTTTAATGTAAAGCAACTATCATCTGATGGAATGATAGGAAAAGTTTGGTATCTACGAGTAGCAGCATCTGAGAAGGAAATAATAGATTCTACAGCTGGGAATACAAAGAGAGAGACTACTTGGATTGTGATTGGAGTACTTGCAGGAATCTTAACTCTCTTTAGCATTGTGTTGGTAGTTGTCAGACGGAGACAGTCTATTCGAGCATTGAAGTCTTTTGAACATTCTCTAGTGCTGTTCAACTACAGAGATTTAAGAAAAGCAACAGAAAACTTCTCAGAGAAACTTGGAGAAGGAGCTTTTGGTTCTGTTTTCAAGGGGATATTGCCTAATTCAACTCCCATAGCAGTGAAACAACTAAAGAGTCTGTTGCAGGGTGAAAAGCAATTCCAGGCTGAAGTGAGAACTCTTGGTGCAATCCAACACATTAATCTTCTTCGCCTCCGTGGGTTTTGTGCAGATTCCACAAGAAGACTCTTGGTTTATGACTACATGCCAAATGGCTCTCTACAATCTGTTCTAGTTCAAAAGCATCCACTAATCTTAGATTGGAAAGCAAGATATCATATTGCCATAGGCATTGCCAGAGGATTGGCTTACCTCCATGAGGACTGTAGATATTGTATAATACACTGTGACATTAAGCCAGAGAACATTTTGTTGGATGAAGAATATAATCCAAAAGTTGGAGATTTTGGTCTTGCAAAACTCATTGGCAGACACCATAGTCGAGTTCTGACAAGTGTGAAAGGAACTGTTGGATATCTAGCACCAGAATGGTTTGCAGGTGAAGCCATCACACCAAAAGCCGATGTCTTCAGCTATGGAAAGTTGCTGTTTGAGGTTATATCTGGAAGGAGAAACAGTGAAGGGTTAGATGATGGATTAGAAAACTACACTCCTTACAGGGTTGCAAATGCAGTAACTAATGGACAAGATGTTCTTACCTTGGTTGATTACAGGTTGGGAGGTAATGCTGACAAGGATGAGCTCATTAGAGCATGCAAAGTTGCGTGTTGGTGTATTCAAGATGAGGAGAAGGATAGGCCAACAATGAGGCAGGTTGTTCAAATTCTCGAAGGAGCTTTAGATGTTGGTATATCCCCGATCCCACAGTTTCTCCAGCACCTTTCAGGAAGTTTACTGGAAGCAATCAATTACCGAGAACCTACTTCAAATTCAGAAATCATATTCCTTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_2 Pkinase S_locus_glycop

Protein Analysis

786

Amino Acids

88.25

Weight (kDa)

6.82

Isoelectric Point (pI)

38.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 76 - 178 5.5e-30 D-mannose binding lectin
S_locus_glycop PF00954 208 - 327 4e-22 S-locus glycoprotein domain
PK_Tyr_Ser-Thr PF07714 478 - 745 2.5e-42 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 479 - 743 1.1e-46 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1934
AasI GACNNNNNNGTC 1 cut(s) 1884
Acc36I ACCTGC 2 cut(s) 1934, 2209
AccB7I CCANNNNNTGG 3 cut(s) 465, 554, 1844
AciI CCGC 3 cut(s) 332, 539, 630
AclWI GGATC 6 cut(s) 134, 260, 758, 869, 882, 2260
AcsI RAATTY 7 cut(s) 349, 370, 859, 977, 1057, 2229, 2335
AcuI CTGAAG 2 cut(s) 1575, 1960
AfaI GTAC 5 cut(s) 70, 206, 432, 797, 1296
AfiI CCNNNNNNNGG 9 cut(s) 465, 554, 774, 792, 855, 950, 1075, 1765, 1844
AhdI GACNNNNNGTC 1 cut(s) 1135
AhlI ACTAGT 1 cut(s) 8
AjnI CCWGG 8 cut(s) 97, 136, 169, 472, 527, 553, 1068, 1548
AjuI GAANNNNNNNTTGG 4 cut(s) 1443, 1475, 1802, 1834
AloI GAACNNNNNNTCC 4 cut(s) 963, 995, 2082, 2114
Alw21I GWGCWC 1 cut(s) 2151
Alw26I GTCTC 4 cut(s) 42, 710, 1265, 1342
AlwI GGATC 6 cut(s) 134, 260, 758, 869, 882, 2260
AlwNI CAGNNNCTG 3 cut(s) 40, 554, 1226
AoxI GGCC 2 cut(s) 100, 2204
ApeKI GCWGC 2 cut(s) 469, 1220
ApoI RAATTY 7 cut(s) 349, 370, 859, 977, 1057, 2229, 2335
ArsI GACNNNNNNTTYG 4 cut(s) 341, 373, 1341, 1373
AseI ATTAAT 1 cut(s) 1587
Asp700I GAANNNNTTC 1 cut(s) 650
AspS9I GGNCC 3 cut(s) 118, 557, 624
AsuHPI GGTGA 5 cut(s) 121, 1084, 1163, 1547, 1958
AsuII TTCGAA 1 cut(s) 969
AvaII GGWCC 3 cut(s) 118, 557, 624
BamHI GGATCC 1 cut(s) 874
BanII GRGCYC 1 cut(s) 2151
BbsI GAAGAC 3 cut(s) 581, 1636, 1966
Bbv12I GWGCWC 1 cut(s) 2151
BbvI GCAGC 2 cut(s) 456, 1232
BccI CCATC 7 cut(s) 392, 666, 865, 1069, 1178, 1961, 2033
BceAI ACGGC 1 cut(s) 859
BciT130I CCWGG 8 cut(s) 99, 138, 171, 474, 529, 555, 1070, 1550
BcoDI GTCTC 4 cut(s) 42, 710, 1265, 1342
BcuI ACTAGT 1 cut(s) 8
BfaI CTAG 5 cut(s) 9, 575, 1388, 1679, 1925
BfmI CTRYAG 5 cut(s) 378, 426, 1248, 1402, 1771
BfuAI ACCTGC 2 cut(s) 1934, 2209
BisI GCNGC 3 cut(s) 333, 470, 1221
BlsI GCNGC 3 cut(s) 334, 471, 1222
BmcAI AGTACT 2 cut(s) 432, 1296
Bme1390I CCNGG 8 cut(s) 99, 138, 171, 474, 529, 555, 1070, 1550
Bme18I GGWCC 3 cut(s) 118, 557, 624
BmeRI GACNNNNNGTC 1 cut(s) 1135
BmgT120I GGNCC 3 cut(s) 118, 557, 624
BmiI GGNNCC 4 cut(s) 119, 559, 847, 876
BmrFI CCNGG 8 cut(s) 99, 138, 171, 474, 529, 555, 1070, 1550
BmrI ACTGGG 2 cut(s) 28, 1000
BmsI GCATC 5 cut(s) 207, 409, 431, 1232, 1699
BmuI ACTGGG 2 cut(s) 28, 1000
BpiI GAAGAC 3 cut(s) 581, 1636, 1966
BplI GAGNNNNNCTC 2 cut(s) 830, 862
BpmI CTGGAG 3 cut(s) 120, 550, 2264
Bpu10I CCTNAGC 1 cut(s) 2354
Bpu14I TTCGAA 1 cut(s) 969
BsaAI YACGTR 1 cut(s) 935
BsaJI CCNNGG 5 cut(s) 527, 944, 1110, 1603, 2106
BsaXI ACNNNNNCTCC 4 cut(s) 2008, 2038, 2262, 2292
Bsc4I CCNNNNNNNGG 9 cut(s) 465, 554, 774, 792, 855, 950, 1075, 1765, 1844
Bse1I ACTGG 5 cut(s) 34, 724, 817, 995, 2307
Bse3DI GCAATG 3 cut(s) 860, 1039, 1737
BseBI CCWGG 8 cut(s) 99, 138, 171, 474, 529, 555, 1070, 1550
BseDI CCNNGG 5 cut(s) 527, 944, 1110, 1603, 2106
BseGI GGATG 5 cut(s) 451, 876, 1690, 1828, 2149
BseLI CCNNNNNNNGG 9 cut(s) 465, 554, 774, 792, 855, 950, 1075, 1765, 1844
BseMI GCAATG 3 cut(s) 860, 1039, 1737
BseMII CTCAG 2 cut(s) 1218, 1449
BseNI ACTGG 5 cut(s) 34, 724, 817, 995, 2307
BseRI GAGGAG 1 cut(s) 2207
BseXI GCAGC 2 cut(s) 456, 1232
BseYI CCCAGC 1 cut(s) 1253
BsgI GTGCAG 1 cut(s) 1635
BshFI GGCC 2 cut(s) 102, 2206
BsiHKAI GWGCWC 1 cut(s) 2151
BslFI GGGAC 2 cut(s) 131, 543
BslI CCNNNNNNNGG 9 cut(s) 465, 554, 774, 792, 855, 950, 1075, 1765, 1844
BsmAI GTCTC 4 cut(s) 42, 710, 1265, 1342
BsmFI GGGAC 2 cut(s) 131, 543
BsmI GAATGC 1 cut(s) 1064
BsnI GGCC 2 cut(s) 102, 2206
Bsp119I TTCGAA 1 cut(s) 969
Bsp1286I GDGCHC 1 cut(s) 2151
Bsp143I GATC 8 cut(s) 126, 265, 448, 763, 874, 1003, 1153, 2265
BspACI CCGC 3 cut(s) 332, 539, 630
BspANI GGCC 2 cut(s) 102, 2206
BspCNI CTCAG 2 cut(s) 1219, 1448
BspLI GGNNCC 4 cut(s) 119, 559, 847, 876
BspMAI CTGCAG 1 cut(s) 430
BspMI ACCTGC 2 cut(s) 1934, 2209
BspPI GGATC 6 cut(s) 134, 260, 758, 869, 882, 2260
BspT104I TTCGAA 1 cut(s) 969
BsrDI GCAATG 3 cut(s) 860, 1039, 1737
BsrI ACTGG 5 cut(s) 34, 724, 817, 995, 2307
BssECI CCNNGG 5 cut(s) 527, 944, 1110, 1603, 2106
BssMI GATC 8 cut(s) 126, 265, 448, 763, 874, 1003, 1153, 2265
BssT1I CCWWGG 2 cut(s) 1110, 2106
Bst2UI CCWGG 8 cut(s) 99, 138, 171, 474, 529, 555, 1070, 1550
Bst4CI ACNGT 7 cut(s) 229, 1353, 1772, 1793, 1915, 2024, 2274
Bst6I CTCTTC 2 cut(s) 669, 1091
BstAPI GCANNNNNTGC 1 cut(s) 746
BstBAI YACGTR 1 cut(s) 935
BstBI TTCGAA 1 cut(s) 969
BstC8I GCNNGC 1 cut(s) 2161
BstDEI CTNAG 6 cut(s) 515, 731, 1227, 1435, 1702, 2354
BstDSI CCRYGG 1 cut(s) 1603
BstENI CCTNNNNNAGG 3 cut(s) 772, 790, 1763
BstF5I GGATG 5 cut(s) 451, 876, 1690, 1828, 2149
BstKTI GATC 8 cut(s) 129, 268, 451, 766, 877, 1006, 1156, 2268
BstMAI GTCTC 4 cut(s) 42, 710, 1265, 1342
BstMBI GATC 8 cut(s) 126, 265, 448, 763, 874, 1003, 1153, 2265
BstMWI GCNNNNNNNGC 3 cut(s) 505, 746, 1949
BstNI CCWGG 8 cut(s) 99, 138, 171, 474, 529, 555, 1070, 1550
BstNSI RCATGY 3 cut(s) 686, 1654, 2163
BstSCI CCNGG 8 cut(s) 97, 136, 169, 472, 527, 553, 1068, 1548
BstSFI CTRYAG 5 cut(s) 378, 426, 1248, 1402, 1771
BstV1I GCAGC 2 cut(s) 456, 1232
BstV2I GAAGAC 3 cut(s) 581, 1636, 1966
BstX2I RGATCY 1 cut(s) 874
BstXI CCANNNNNNTGG 1 cut(s) 1750
BstYI RGATCY 1 cut(s) 874
BsuRI GGCC 2 cut(s) 102, 2206
BtgI CCRYGG 1 cut(s) 1603
BtsCI GGATG 5 cut(s) 451, 876, 1690, 1828, 2149
BtsI GCAGTG 2 cut(s) 1051, 1512
BtsIMutI CAGTG 5 cut(s) 810, 1051, 1512, 1789, 2029
BveI ACCTGC 2 cut(s) 1934, 2209
Cac8I GCNNGC 1 cut(s) 2161
CaiI CAGNNNCTG 3 cut(s) 40, 554, 1226
Cfr13I GGNCC 3 cut(s) 118, 557, 624
CsiI ACCWGGT 2 cut(s) 169, 553
Csp6I GTAC 5 cut(s) 69, 205, 431, 796, 1295
CviAII CATG 7 cut(s) 385, 683, 799, 835, 1651, 1763, 2160
CviQI GTAC 5 cut(s) 69, 205, 431, 796, 1295
DdeI CTNAG 6 cut(s) 515, 731, 1227, 1435, 1702, 2354
DpnI GATC 8 cut(s) 128, 267, 450, 765, 876, 1005, 1155, 2267
DpnII GATC 8 cut(s) 126, 265, 448, 763, 874, 1003, 1153, 2265
DraI TTTAAA 1 cut(s) 711
DrdI GACNNNNNNGTC 1 cut(s) 1884
DriI GACNNNNNGTC 1 cut(s) 1135
DseDI GACNNNNNNGTC 1 cut(s) 1884
Eam1104I CTCTTC 2 cut(s) 669, 1091
Eam1105I GACNNNNNGTC 1 cut(s) 1135
EarI CTCTTC 2 cut(s) 669, 1091
Ecl136II GAGCTC 1 cut(s) 2149
Eco130I CCWWGG 2 cut(s) 1110, 2106
Eco24I GRGCYC 1 cut(s) 2151
Eco32I GATATC 2 cut(s) 1721, 1922
Eco47I GGWCC 3 cut(s) 118, 557, 624
Eco53kI GAGCTC 1 cut(s) 2149
Eco57I CTGAAG 2 cut(s) 1575, 1960
EcoICRI GAGCTC 1 cut(s) 2149
EcoNI CCTNNNNNAGG 3 cut(s) 772, 790, 1763
EcoRI GAATTC 1 cut(s) 1057
EcoRII CCWGG 8 cut(s) 97, 136, 169, 472, 527, 553, 1068, 1548
EcoRV GATATC 2 cut(s) 1721, 1922
EcoT14I CCWWGG 2 cut(s) 1110, 2106
EcoT22I ATGCAT 1 cut(s) 424
EcoT38I GRGCYC 1 cut(s) 2151
ErhI CCWWGG 2 cut(s) 1110, 2106
FaeI CATG 7 cut(s) 388, 686, 802, 838, 1654, 1766, 2163
FalI AAGNNNNNCTT 2 cut(s) 2085, 2117
FaqI GGGAC 2 cut(s) 131, 543
FatI CATG 7 cut(s) 384, 682, 798, 834, 1650, 1762, 2159
Fnu4HI GCNGC 3 cut(s) 333, 470, 1221
FokI GGATG 5 cut(s) 438, 883, 1677, 1835, 2156
FriOI GRGCYC 1 cut(s) 2151
Fsp4HI GCNGC 3 cut(s) 333, 470, 1221
FspBI CTAG 5 cut(s) 9, 575, 1388, 1679, 1925
GluI GCNGC 3 cut(s) 333, 470, 1221
GsaI CCCAGC 1 cut(s) 1257
GsuI CTGGAG 3 cut(s) 120, 550, 2264
HaeIII GGCC 2 cut(s) 102, 2206
Hin1II CATG 7 cut(s) 388, 686, 802, 838, 1654, 1766, 2163
HincII GTYRAC 1 cut(s) 345
HindII GTYRAC 1 cut(s) 345
HindIII AAGCTT 1 cut(s) 497
HinfI GANTC 9 cut(s) 778, 966, 1099, 1106, 1244, 1305, 1522, 1619, 1632
HphI GGTGA 5 cut(s) 121, 1084, 1163, 1547, 1958
Hpy166II GTNNAC 2 cut(s) 345, 2300
Hpy188I TCNGA 9 cut(s) 17, 613, 1141, 1183, 1228, 1343, 1438, 1896, 2341
Hpy188III TCNNGA 8 cut(s) 392, 665, 761, 1103, 2010, 2186, 2234, 2292
Hpy8I GTNNAC 2 cut(s) 345, 2300
HpyAV CCTTC 6 cut(s) 1225, 1445, 2007, 2021, 2191, 2231
HpyCH4III ACNGT 7 cut(s) 229, 1353, 1772, 1793, 1915, 2024, 2274
HpyCH4IV ACGT 2 cut(s) 356, 934
HpyF10VI GCNNNNNNNGC 3 cut(s) 505, 746, 1949
HpyF3I CTNAG 6 cut(s) 515, 731, 1227, 1435, 1702, 2354
HpySE526I ACGT 2 cut(s) 356, 934
Hsp92II CATG 7 cut(s) 388, 686, 802, 838, 1654, 1766, 2163
Kzo9I GATC 8 cut(s) 126, 265, 448, 763, 874, 1003, 1153, 2265
LmnI GCTCC 3 cut(s) 851, 1454, 2240
Lsp1109I GCAGC 2 cut(s) 456, 1232
LweI GCATC 5 cut(s) 207, 409, 431, 1232, 1699
MabI ACCWGGT 2 cut(s) 169, 553
MaeI CTAG 5 cut(s) 9, 575, 1388, 1679, 1925
MaeII ACGT 2 cut(s) 356, 934
MaeIII GTNAC 6 cut(s) 93, 173, 357, 1084, 1793, 2080
MalI GATC 8 cut(s) 128, 267, 450, 765, 876, 1005, 1155, 2267
MboI GATC 8 cut(s) 126, 265, 448, 763, 874, 1003, 1153, 2265
MflI RGATCY 1 cut(s) 874
MhlI GDGCHC 1 cut(s) 2151
MlyI GAGTC 4 cut(s) 1100, 1108, 1531, 1626
MmeI TCCRAC 5 cut(s) 479, 1603, 1800, 1825, 1897
Mph1103I ATGCAT 1 cut(s) 424
MroXI GAANNNNTTC 1 cut(s) 650
MseI TTAA 7 cut(s) 314, 710, 1161, 1310, 1412, 1587, 1800
MslI CAYNNNNRTG 4 cut(s) 1011, 1044, 1505, 1933
MspA1I CMGCKG 3 cut(s) 332, 630, 1253
MspR9I CCNGG 8 cut(s) 99, 138, 171, 474, 529, 555, 1070, 1550
Mva1269I GAATGC 1 cut(s) 1064
MvaI CCWGG 8 cut(s) 99, 138, 171, 474, 529, 555, 1070, 1550
MwoI GCNNNNNNNGC 3 cut(s) 505, 746, 1949
NdeII GATC 8 cut(s) 126, 265, 448, 763, 874, 1003, 1153, 2265
NlaIII CATG 7 cut(s) 388, 686, 802, 838, 1654, 1766, 2163
NlaIV GGNNCC 4 cut(s) 119, 559, 847, 876
NmuCI GTSAC 2 cut(s) 357, 1793
NsiI ATGCAT 1 cut(s) 424
NspI RCATGY 3 cut(s) 686, 1654, 2163
NspV TTCGAA 1 cut(s) 969
PaeI GCATGC 1 cut(s) 2163
PaqCI CACCTGC 1 cut(s) 1934
PctI GAATGC 1 cut(s) 1064
PdmI GAANNNNTTC 1 cut(s) 650
PfeI GAWTC 5 cut(s) 778, 966, 1244, 1305, 1619
PflMI CCANNNNNTGG 3 cut(s) 465, 554, 1844
PkrI GCNGC 3 cut(s) 334, 471, 1222
PleI GAGTC 4 cut(s) 1100, 1107, 1530, 1626
PpsI GAGTC 4 cut(s) 1100, 1107, 1530, 1626
Ppu21I YACGTR 1 cut(s) 935
PshBI ATTAAT 1 cut(s) 1587
Psp124BI GAGCTC 1 cut(s) 2151
Psp6I CCWGG 8 cut(s) 97, 136, 169, 472, 527, 553, 1068, 1548
PspFI CCCAGC 1 cut(s) 1253
PspGI CCWGG 8 cut(s) 97, 136, 169, 472, 527, 553, 1068, 1548
PspN4I GGNNCC 4 cut(s) 119, 559, 847, 876
PspPI GGNCC 3 cut(s) 118, 557, 624
PstI CTGCAG 1 cut(s) 430
PstNI CAGNNNCTG 3 cut(s) 40, 554, 1226
PsuI RGATCY 1 cut(s) 874
PvuII CAGCTG 1 cut(s) 1253
RsaI GTAC 5 cut(s) 70, 206, 432, 797, 1296
RsaNI GTAC 5 cut(s) 69, 205, 431, 796, 1295
RseI CAYNNNNRTG 4 cut(s) 1011, 1044, 1505, 1933
SacI GAGCTC 1 cut(s) 2151
SaqAI TTAA 7 cut(s) 314, 710, 1161, 1310, 1412, 1587, 1800
SatI GCNGC 3 cut(s) 333, 470, 1221
Sau3AI GATC 8 cut(s) 126, 265, 448, 763, 874, 1003, 1153, 2265
Sau96I GGNCC 3 cut(s) 118, 557, 624
ScaI AGTACT 2 cut(s) 432, 1296
SchI GAGTC 4 cut(s) 1100, 1108, 1531, 1626
ScrFI CCNGG 8 cut(s) 99, 138, 171, 474, 529, 555, 1070, 1550
SduI GDGCHC 1 cut(s) 2151
SexAI ACCWGGT 2 cut(s) 169, 553
SfaNI GCATC 5 cut(s) 207, 409, 431, 1232, 1699
SfcI CTRYAG 5 cut(s) 378, 426, 1248, 1402, 1771
SfuI TTCGAA 1 cut(s) 969
SinI GGWCC 3 cut(s) 118, 557, 624
SmiMI CAYNNNNRTG 4 cut(s) 1011, 1044, 1505, 1933
SpeI ACTAGT 1 cut(s) 8
SphI GCATGC 1 cut(s) 2163
SsiI CCGC 3 cut(s) 332, 539, 630
SspMI CTAG 5 cut(s) 9, 575, 1388, 1679, 1925
SstI GAGCTC 1 cut(s) 2151
StyD4I CCNGG 8 cut(s) 97, 136, 169, 472, 527, 553, 1068, 1548
StyI CCWWGG 2 cut(s) 1110, 2106
TaaI ACNGT 7 cut(s) 229, 1353, 1772, 1793, 1915, 2024, 2274
TaiI ACGT 2 cut(s) 359, 937
TaqI TCGA 5 cut(s) 447, 969, 1360, 1888, 2235
TatI WGTACW 3 cut(s) 68, 430, 1294
TauI GCSGC 1 cut(s) 335
TfiI GAWTC 5 cut(s) 778, 966, 1244, 1305, 1619
Tru1I TTAA 7 cut(s) 314, 710, 1161, 1310, 1412, 1587, 1800
Tru9I TTAA 7 cut(s) 314, 710, 1161, 1310, 1412, 1587, 1800
TscAI CASTG 5 cut(s) 817, 1051, 1512, 1796, 2029
TseFI GTSAC 2 cut(s) 357, 1793
TseI GCWGC 2 cut(s) 469, 1220
Tsp45I GTSAC 2 cut(s) 357, 1793
TspDTI ATGAA 4 cut(s) 17, 836, 1028, 1839
TspGWI ACGGA 2 cut(s) 1360, 1592
TspRI CASTG 5 cut(s) 817, 1051, 1512, 1796, 2029
Van91I CCANNNNNTGG 3 cut(s) 465, 554, 1844
VpaK11BI GGWCC 3 cut(s) 118, 557, 624
VspI ATTAAT 1 cut(s) 1587
XagI CCTNNNNNAGG 3 cut(s) 772, 790, 1763
XapI RAATTY 7 cut(s) 349, 370, 859, 977, 1057, 2229, 2335
XceI RCATGY 3 cut(s) 686, 1654, 2163
XcmI CCANNNNNNNNNTGG 2 cut(s) 110, 462
XmnI GAANNNNTTC 1 cut(s) 650
XspI CTAG 5 cut(s) 9, 575, 1388, 1679, 1925
ZrmI AGTACT 2 cut(s) 432, 1296
Zsp2I ATGCAT 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.