pycom14g03020

G-type lectin S-receptor-like serine threonine-protein kinase At2g19130

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0000916
Physical Location & Seq
Reverse (-)
2336406 .. 2346485
10080 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g03020.3

Sequence Viewer

Length: 2313 bp
ATGGATACGAAAATTCTACTGTGCTATACGTTGAGATTTTTGTTATGCTGTACCTTCATTGCTTGTATTTCCATGGCGTCTCACACAATCTCTCCAGGTCAATCTCTCTCCGGGGCACGAACAATCACTTCTCCAAGCGGAGCATTTGAGCTGGGTTTCTTCACACCAGGTAATTCTCGAAACAGTTACGTAGGCATTTGGTACACAAATGAAACCATTAAGACCGTAGTTTGGGTCGCAAACAGGGAACAACCTGTTTCTGAACTGAAACTTTCAGAATTAGCACTTGTCGAAAATGGAAACTTGATCTTAAGTAGCCCCTCCGAAGTTGCAATATGGTCGACTGATTCAAGATCAAAGGTCTCTAATTCGAGTGTAGCAAAGCTTCTTGACAATGGGAACTTTGTCATAACAGATGCATTTGATTCATCTGTTGTTATATGGCAGAGTTTTGATCACCTGTCGGATACTTGGCTGCCGGGTGCTGAGATGTACAATTATCGCACCGAGAAAATACTAGTTCTTACATCCTGGAGAAACGTGGAAAGTCCTGCACCTGGTCATTTTTCTTTTGAGCTGGATGAATTACCCTTTAATTTGTCTGACTATCGTGGTGCCTTTATTAGAGGCACACTTGAAATCAATGGTGAGCTCAGGTTTTATGCCATGTACCAACGCTATGGGGGATGGACTTTGATTTCAGGTGAACCAGTGAACCGATGTGATATCGATGCTTTCTGTGGTGCTTTTAGTTATTGCTACCCGTACGGTTCTCCTGCTTGTCGTTGCCTCGAAGGATTTGAACCCAAAGTCATGGAAAATTGGAGACTAGAGTACTTCTCAGATGGCTGTGTGAGGAAAACTCCTTTTCACTGCAGTCCTGGGGAAAGTTACTCATTTTTGGCAATAACTGATGCTGCATATGGATATCCTAATAAGTATGAGATAATTTCGAATGTAAGCAATGATCAATGCAGATTAGAATGCTCAAGAAAGTGCTCATGCGCTGCCTTTTTTTATAATTCTTACGATAGTGGTCGGTGTTACATGTGGATAGGGGATGTATATAATAAACAATTTCTGAGAGTTCTTAGGTTCGAATCTCGTGGATATGAGACGAAGAGAAAGACTACTTGGATTGTACTTGGAGTACTTGCAGGGTTCTTTTCCATCTTAAGTGCTGCCACTCTCATCGTATTTTTTAGAAGGAGATGGTCAGCGGCAGCATTGGCGACTACCGATGATTCTTTGGTGCTTTACAAGCATAGAGATTTAAGACGAGCAACAAAGAACTTCTCCGAAAAACTCGGGGAAGGAGCCTTTGGTTCTGTATTCAAGGGGATGCTGCCTAACTCAACAGCCATAGCAGTGAAACAGCTGAAATGTATGGACCAGGGAGAGAAACAATTTCGTGCTGAAGTGGGAACTATCGGTGTAATCCAACACAATAATCTTATTCGCATGCGGGGATTCTGCACGAATGCTTCAAAGAGGTTTTTGGTTTATGACTACATGCCAAATGGTTCTCTGCAATCTCTTCTACTTGGAAAGAACCCGACAATGTTAGATTGGAAGGCTAGATATCACATTGCAGTAGGGACTGCCAAAGGATTGGCTTACCTCCATGAAGAGTGCAGAGACTGCATAATACACTGCGATGTTAAGCCGGAGAACATCTTGTTGGATTCAGAATACAGTCCGAAATTGGCAGATTTTGGTCTTGCGAAACTTTTGGGGAGACATCACAGCCGGGTTTTGACAACCATGAGAGGAACTGTGGGCTATCTCGCACCAGAATGGTTTTCGGGTGAAGCTATCACGCCAAAAGCCGATGTGTTTAGCTATGGCATGTTGCTGATTGAGGTCATATCAGGGAGGAGAAACAGAGAAGGGTTAGATGACGGTATAGAAAACTTCCAACCTATCCGCCTCGCGAATGCAGTTAATAAAGGGGAAGATGTTTTTGCCTTGTTGGATTGCAGGTTGGAAGGCAAAGCTGACAGAGATGAACTAAGTAGAGCGTGCAAAGTGGCTTGTTGGTGCATTCAAGAAGATGAGAAGGATAGGCCAACAATGAGGCAGGTGATTCAAATTCTTGAGGGAGTTTCGGACATCGGTGTTCCACCAGTCCCGCAGTTCCTCCAGCGCCTTATCGGAGGTCCGTCAGAAGGCATTAACTACCAGGAGACTACGTCCGGTTCGGGTTCATGGTCATGCTCCGATGTCCAAAGTGCATTTCAAGCCCAAGTCAGCAACTGTGCAACTAAAAGTCATGAGGATGAGATGAAGAACGTGAGGGTTCTTGTATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

771

Amino Acids

86.41

Weight (kDa)

6.5

Isoelectric Point (pI)

44.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1020
AarI CACCTGC 1 cut(s) 2071
Acc36I ACCTGC 2 cut(s) 1971, 2071
AccB1I GGYRCC 1 cut(s) 614
AccI GTMKAC 1 cut(s) 341
AccII CGCG 1 cut(s) 1934
AciI CCGC 5 cut(s) 138, 1220, 1465, 1927, 2132
AcsI RAATTY 2 cut(s) 12, 2091
AcuI CTGAAG 1 cut(s) 1437
AcyI GRCGYC 1 cut(s) 77
AfaI GTAC 8 cut(s) 52, 203, 494, 671, 767, 836, 1143, 1152
AfiI CCNNNNNNNGG 3 cut(s) 231, 557, 2168
AflII CTTAAG 2 cut(s) 310, 1174
AflIII ACRYGT 1 cut(s) 1047
AgsI TTSAA 8 cut(s) 351, 638, 803, 1336, 1488, 2048, 2090, 2240
AhlI ACTAGT 1 cut(s) 517
AjnI CCWGG 7 cut(s) 94, 166, 530, 556, 880, 1392, 2181
AjuI GAANNNNNNNTTGG 8 cut(s) 1117, 1149, 1305, 1337, 1664, 1696, 2220, 2252
AluBI AGCT 8 cut(s) 151, 385, 577, 652, 1378, 1814, 1842, 1997
AluI AGCT 8 cut(s) 151, 385, 577, 652, 1378, 1814, 1842, 1997
Alw21I GWGCWC 2 cut(s) 654, 1001
Alw26I GTCTC 7 cut(s) 84, 367, 820, 1109, 1632, 1732, 2180
AlwNI CAGNNNCTG 2 cut(s) 1641, 2256
Ama87I CYCGRG 1 cut(s) 1307
AoxI GGCC 1 cut(s) 2066
ApeKI GCWGC 6 cut(s) 475, 917, 1007, 1181, 1223, 1345
ApoI RAATTY 2 cut(s) 12, 2091
AspLEI GCGC 2 cut(s) 1007, 2148
AspS9I GGNCC 2 cut(s) 1390, 2159
AsuC2I CCSGG 3 cut(s) 112, 480, 1751
AsuHPI GGTGA 5 cut(s) 449, 659, 716, 1820, 2095
AsuII TTCGAA 2 cut(s) 953, 1098
AvaI CYCGRG 1 cut(s) 1307
AvaII GGWCC 2 cut(s) 1390, 2159
BaeGI GKGCMC 1 cut(s) 118
BanI GGYRCC 1 cut(s) 614
BanII GRGCYC 1 cut(s) 654
BarI GAAGNNNNNNTAC 2 cut(s) 369, 401
BauI CACGAG 1 cut(s) 1104
Bbv12I GWGCWC 2 cut(s) 654, 1001
BbvI GCAGC 6 cut(s) 462, 904, 994, 1168, 1235, 1332
BccI CCATC 4 cut(s) 681, 839, 1178, 1206
BcgI CGANNNNNNTGC 2 cut(s) 321, 355
BciT130I CCWGG 7 cut(s) 96, 168, 532, 558, 882, 1394, 2183
BciVI GTATCC 1 cut(s) 460
BclI TGATCA 2 cut(s) 454, 967
BcnI CCSGG 3 cut(s) 112, 480, 1751
BcoDI GTCTC 7 cut(s) 84, 367, 820, 1109, 1632, 1732, 2180
BcuI ACTAGT 1 cut(s) 517
BfaI CTAG 3 cut(s) 518, 830, 1578
BfmI CTRYAG 1 cut(s) 874
BfoI RGCGCY 1 cut(s) 2149
BfrI CTTAAG 2 cut(s) 310, 1174
BfuAI ACCTGC 2 cut(s) 1971, 2071
BfuI GTATCC 1 cut(s) 460
BisI GCNGC 7 cut(s) 476, 918, 1008, 1182, 1221, 1224, 1346
BlsI GCNGC 7 cut(s) 477, 919, 1009, 1183, 1222, 1225, 1347
BmcAI AGTACT 2 cut(s) 836, 1152
Bme18I GGWCC 2 cut(s) 1390, 2159
BmeT110I CYCGRG 1 cut(s) 1307
BmgT120I GGNCC 2 cut(s) 1390, 2159
BmiI GGNNCC 2 cut(s) 616, 1318
BmsI GCATC 4 cut(s) 406, 721, 904, 1332
BplI GAGNNNNNCTC 4 cut(s) 824, 856, 847, 879
BpmI CTGGAG 3 cut(s) 78, 553, 2126
Bpu10I CCTNAGC 1 cut(s) 653
Bpu14I TTCGAA 2 cut(s) 953, 1098
BpuEI CTTGAG 2 cut(s) 973, 2117
BpuMI CCSGG 3 cut(s) 112, 480, 1751
Bsa29I ATCGAT 1 cut(s) 729
BsaAI YACGTR 1 cut(s) 190
BsaHI GRCGYC 1 cut(s) 77
BsaI GGTCTC 1 cut(s) 367
BsaJI CCNNGG 4 cut(s) 72, 111, 881, 1393
BsaWI WCCGGW 1 cut(s) 2195
BsaXI ACNNNNNCTCC 4 cut(s) 76, 106, 2177, 2207
Bsc4I CCNNNNNNNGG 3 cut(s) 231, 557, 2168
Bse1I ACTGG 2 cut(s) 710, 2126
Bse3DI GCAATG 3 cut(s) 57, 970, 1587
BseBI CCWGG 7 cut(s) 96, 168, 532, 558, 882, 1394, 2183
BseCI ATCGAT 1 cut(s) 729
BseDI CCNNGG 4 cut(s) 72, 111, 881, 1393
BseGI GGATG 6 cut(s) 527, 586, 692, 1066, 1347, 2284
BseLI CCNNNNNNNGG 3 cut(s) 231, 557, 2168
BseMI GCAATG 3 cut(s) 57, 970, 1587
BseMII CTCAG 4 cut(s) 477, 667, 855, 1073
BseNI ACTGG 2 cut(s) 710, 2126
BseRI GAGGAG 1 cut(s) 1891
BseSI GKGCMC 1 cut(s) 118
BseXI GCAGC 6 cut(s) 462, 904, 994, 1168, 1235, 1332
BseYI CCCAGC 1 cut(s) 151
BsgI GTGCAG 3 cut(s) 537, 1459, 1654
Bsh1236I CGCG 1 cut(s) 1934
BshFI GGCC 1 cut(s) 2068
BshNI GGYRCC 1 cut(s) 614
BshVI ATCGAT 1 cut(s) 729
BsiHKAI GWGCWC 2 cut(s) 654, 1001
BsiHKCI CYCGRG 1 cut(s) 1307
BsiSI CCGG 5 cut(s) 111, 479, 1667, 1750, 2196
BsiWI CGTACG 1 cut(s) 765
BslFI GGGAC 2 cut(s) 1612, 2114
BslI CCNNNNNNNGG 3 cut(s) 231, 557, 2168
BsmAI GTCTC 7 cut(s) 84, 367, 820, 1109, 1632, 1732, 2180
BsmBI CGTCTC 2 cut(s) 84, 1109
BsmFI GGGAC 2 cut(s) 1612, 2114
BsmI GAATGC 4 cut(s) 989, 1486, 1942, 2043
BsnI GGCC 1 cut(s) 2068
Bso31I GGTCTC 1 cut(s) 367
BsoBI CYCGRG 1 cut(s) 1307
Bsp119I TTCGAA 2 cut(s) 953, 1098
Bsp1286I GDGCHC 3 cut(s) 118, 654, 1001
Bsp1407I TGTACA 1 cut(s) 492
Bsp143I GATC 4 cut(s) 306, 353, 454, 967
Bsp19I CCATGG 1 cut(s) 72
Bsp68I TCGCGA 1 cut(s) 1934
BspACI CCGC 5 cut(s) 138, 1220, 1465, 1927, 2132
BspANI GGCC 1 cut(s) 2068
BspCNI CTCAG 4 cut(s) 478, 666, 854, 1074
BspDI ATCGAT 1 cut(s) 729
BspFNI CGCG 1 cut(s) 1934
BspHI TCATGA 1 cut(s) 2272
BspLI GGNNCC 2 cut(s) 616, 1318
BspMAI CTGCAG 1 cut(s) 878
BspMI ACCTGC 2 cut(s) 1971, 2071
BspT104I TTCGAA 2 cut(s) 953, 1098
BspT107I GGYRCC 1 cut(s) 614
BspTI CTTAAG 2 cut(s) 310, 1174
BspTNI GGTCTC 1 cut(s) 367
BsrDI GCAATG 3 cut(s) 57, 970, 1587
BsrGI TGTACA 1 cut(s) 492
BsrI ACTGG 2 cut(s) 710, 2126
BssECI CCNNGG 4 cut(s) 72, 111, 881, 1393
BssMI GATC 4 cut(s) 306, 353, 454, 967
BssNI GRCGYC 1 cut(s) 77
BssSI CACGAG 1 cut(s) 1104
BssT1I CCWWGG 1 cut(s) 72
Bst2BI CACGAG 1 cut(s) 1104
Bst2UI CCWGG 7 cut(s) 96, 168, 532, 558, 882, 1394, 2183
Bst4CI ACNGT 8 cut(s) 21, 185, 226, 770, 1697, 1777, 1904, 2258
Bst6I CTCTTC 3 cut(s) 1115, 1542, 1623
BstACI GRCGYC 1 cut(s) 77
BstAFI CTTAAG 2 cut(s) 310, 1174
BstAPI GCANNNNNTGC 1 cut(s) 1641
BstAUI TGTACA 1 cut(s) 492
BstBAI YACGTR 1 cut(s) 190
BstBI TTCGAA 2 cut(s) 953, 1098
BstC8I GCNNGC 2 cut(s) 1463, 2023
BstDEI CTNAG 6 cut(s) 486, 653, 841, 1082, 1091, 2012
BstDSI CCRYGG 1 cut(s) 72
BstF5I GGATG 6 cut(s) 527, 586, 692, 1066, 1347, 2284
BstFNI CGCG 1 cut(s) 1934
BstH2I RGCGCY 1 cut(s) 2149
BstHHI GCGC 2 cut(s) 1007, 2148
BstKTI GATC 4 cut(s) 309, 356, 457, 970
BstMAI GTCTC 7 cut(s) 84, 367, 820, 1109, 1632, 1732, 2180
BstMBI GATC 4 cut(s) 306, 353, 454, 967
BstMWI GCNNNNNNNGC 4 cut(s) 1229, 1261, 1641, 2240
BstNI CCWGG 7 cut(s) 96, 168, 532, 558, 882, 1394, 2183
BstNSI RCATGY 4 cut(s) 1051, 1465, 1516, 1852
BstSFI CTRYAG 1 cut(s) 874
BstSLI GKGCMC 1 cut(s) 118
BstSNI TACGTA 1 cut(s) 190
BstUI CGCG 1 cut(s) 1934
BstV1I GCAGC 6 cut(s) 462, 904, 994, 1168, 1235, 1332
BstXI CCANNNNNNTGG 3 cut(s) 680, 814, 1612
Bsu15I ATCGAT 1 cut(s) 729
BsuI GTATCC 1 cut(s) 460
BsuRI GGCC 1 cut(s) 2068
BsuTUI ATCGAT 1 cut(s) 729
BtgI CCRYGG 1 cut(s) 72
BtgZI GCGATG 1 cut(s) 1671
BtsCI GGATG 6 cut(s) 527, 586, 692, 1066, 1347, 2284
BtsI GCAGTG 3 cut(s) 871, 1374, 1651
BtsIMutI CAGTG 4 cut(s) 717, 871, 1374, 1651
BtuMI TCGCGA 1 cut(s) 1934
BveI ACCTGC 2 cut(s) 1971, 2071
Cac8I GCNNGC 2 cut(s) 1463, 2023
CaiI CAGNNNCTG 2 cut(s) 1641, 2256
CciI TCATGA 1 cut(s) 2272
CfoI GCGC 2 cut(s) 1007, 2148
Cfr13I GGNCC 2 cut(s) 1390, 2159
ClaI ATCGAT 1 cut(s) 729
CseI GACGC 1 cut(s) 66
CsiI ACCWGGT 2 cut(s) 166, 556
Csp6I GTAC 8 cut(s) 51, 202, 493, 670, 766, 835, 1142, 1151
CviQI GTAC 8 cut(s) 51, 202, 493, 670, 766, 835, 1142, 1151
DdeI CTNAG 6 cut(s) 486, 653, 841, 1082, 1091, 2012
DpnI GATC 4 cut(s) 308, 355, 456, 969
DpnII GATC 4 cut(s) 306, 353, 454, 967
Eam1104I CTCTTC 3 cut(s) 1115, 1542, 1623
EarI CTCTTC 3 cut(s) 1115, 1542, 1623
EciI GGCGGA 1 cut(s) 1916
Ecl136II GAGCTC 1 cut(s) 652
Eco105I TACGTA 1 cut(s) 190
Eco130I CCWWGG 1 cut(s) 72
Eco24I GRGCYC 1 cut(s) 654
Eco31I GGTCTC 1 cut(s) 367
Eco32I GATATC 3 cut(s) 727, 929, 1583
Eco47I GGWCC 2 cut(s) 1390, 2159
Eco53kI GAGCTC 1 cut(s) 652
Eco57I CTGAAG 1 cut(s) 1437
Eco88I CYCGRG 1 cut(s) 1307
EcoICRI GAGCTC 1 cut(s) 652
EcoRII CCWGG 7 cut(s) 94, 166, 530, 556, 880, 1392, 2181
EcoRV GATATC 3 cut(s) 727, 929, 1583
EcoT14I CCWWGG 1 cut(s) 72
EcoT22I ATGCAT 1 cut(s) 421
EcoT38I GRGCYC 1 cut(s) 654
ErhI CCWWGG 1 cut(s) 72
Esp3I CGTCTC 2 cut(s) 84, 1109
FaqI GGGAC 2 cut(s) 1612, 2114
FauI CCCGC 2 cut(s) 1458, 2139
FauNDI CATATG 1 cut(s) 922
FbaI TGATCA 2 cut(s) 454, 967
FblI GTMKAC 1 cut(s) 341
Fnu4HI GCNGC 7 cut(s) 476, 918, 1008, 1182, 1221, 1224, 1346
FokI GGATG 6 cut(s) 514, 593, 699, 1073, 1354, 2291
FriOI GRGCYC 1 cut(s) 654
Fsp4HI GCNGC 7 cut(s) 476, 918, 1008, 1182, 1221, 1224, 1346
FspBI CTAG 3 cut(s) 518, 830, 1578
GlaI GCGC 2 cut(s) 1006, 2147
GluI GCNGC 7 cut(s) 476, 918, 1008, 1182, 1221, 1224, 1346
GsaI CCCAGC 1 cut(s) 155
GsuI CTGGAG 3 cut(s) 78, 553, 2126
HaeII RGCGCY 1 cut(s) 2149
HaeIII GGCC 1 cut(s) 2068
HapII CCGG 5 cut(s) 111, 479, 1667, 1750, 2196
HgaI GACGC 1 cut(s) 66
HhaI GCGC 2 cut(s) 1007, 2148
Hin1I GRCGYC 1 cut(s) 77
Hin6I GCGC 2 cut(s) 1005, 2146
HinP1I GCGC 2 cut(s) 1005, 2146
HincII GTYRAC 1 cut(s) 342
HindII GTYRAC 1 cut(s) 342
HindIII AAGCTT 1 cut(s) 383
HinfI GANTC 7 cut(s) 347, 425, 1100, 1244, 1470, 1685, 2086
HpaII CCGG 5 cut(s) 111, 479, 1667, 1750, 2196
HphI GGTGA 5 cut(s) 449, 659, 716, 1820, 2095
Hpy166II GTNNAC 4 cut(s) 204, 342, 707, 715
Hpy188III TCNNGA 8 cut(s) 177, 351, 389, 990, 1933, 2048, 2096, 2273
Hpy8I GTNNAC 4 cut(s) 204, 342, 707, 715
HpyAV CCTTC 9 cut(s) 64, 788, 1200, 1307, 1567, 1883, 1982, 2053, 2162
HpyCH4III ACNGT 8 cut(s) 21, 185, 226, 770, 1697, 1777, 1904, 2258
HpyCH4IV ACGT 5 cut(s) 29, 189, 540, 2192, 2292
HpyF10VI GCNNNNNNNGC 4 cut(s) 1229, 1261, 1641, 2240
HpyF3I CTNAG 6 cut(s) 486, 653, 841, 1082, 1091, 2012
HpySE526I ACGT 5 cut(s) 29, 189, 540, 2192, 2292
Hsp92I GRCGYC 1 cut(s) 77
HspAI GCGC 2 cut(s) 1005, 2146
Ksp22I TGATCA 2 cut(s) 454, 967
Kzo9I GATC 4 cut(s) 306, 353, 454, 967
LmnI GCTCC 3 cut(s) 140, 1316, 2222
Lsp1109I GCAGC 6 cut(s) 462, 904, 994, 1168, 1235, 1332
LweI GCATC 4 cut(s) 406, 721, 904, 1332
MabI ACCWGGT 2 cut(s) 166, 556
MaeI CTAG 3 cut(s) 518, 830, 1578
MaeII ACGT 5 cut(s) 29, 189, 540, 2192, 2292
MaeIII GTNAC 3 cut(s) 185, 890, 1043
MalI GATC 4 cut(s) 308, 355, 456, 969
MboI GATC 4 cut(s) 306, 353, 454, 967
MboII GAAGA 7 cut(s) 151, 1132, 1529, 1640, 1967, 2063, 2299
MhlI GDGCHC 3 cut(s) 118, 654, 1001
MmeI TCCRAC 6 cut(s) 444, 1465, 1662, 1942, 1953, 1965
Mph1103I ATGCAT 1 cut(s) 421
MseI TTAA 8 cut(s) 219, 311, 594, 1175, 1274, 1662, 1944, 2175
MslI CAYNNNNRTG 5 cut(s) 1367, 1656, 1795, 2212, 2277
MspA1I CMGCKG 2 cut(s) 1220, 1378
MspCI CTTAAG 2 cut(s) 310, 1174
MspI CCGG 5 cut(s) 111, 479, 1667, 1750, 2196
Mva1269I GAATGC 4 cut(s) 989, 1486, 1942, 2043
MvaI CCWGG 7 cut(s) 96, 168, 532, 558, 882, 1394, 2183
MvnI CGCG 1 cut(s) 1934
MwoI GCNNNNNNNGC 4 cut(s) 1229, 1261, 1641, 2240
NciI CCSGG 3 cut(s) 112, 480, 1751
NcoI CCATGG 1 cut(s) 72
NdeI CATATG 1 cut(s) 922
NdeII GATC 4 cut(s) 306, 353, 454, 967
NlaIV GGNNCC 2 cut(s) 616, 1318
NruI TCGCGA 1 cut(s) 1934
NsiI ATGCAT 1 cut(s) 421
NspI RCATGY 4 cut(s) 1051, 1465, 1516, 1852
NspV TTCGAA 2 cut(s) 953, 1098
PaeI GCATGC 1 cut(s) 1465
PagI TCATGA 1 cut(s) 2272
PaqCI CACCTGC 1 cut(s) 2071
PciI ACATGT 1 cut(s) 1047
PctI GAATGC 4 cut(s) 989, 1486, 1942, 2043
PfeI GAWTC 7 cut(s) 347, 425, 1100, 1244, 1470, 1685, 2086
Pfl23II CGTACG 1 cut(s) 765
PflFI GACNNNGTC 1 cut(s) 2191
PfoI TCCNGGA 1 cut(s) 530
PkrI GCNGC 7 cut(s) 477, 919, 1009, 1183, 1222, 1225, 1347
Ppu21I YACGTR 1 cut(s) 190
PscI ACATGT 1 cut(s) 1047
PsiI TTATAA 1 cut(s) 1020
Psp124BI GAGCTC 1 cut(s) 654
Psp6I CCWGG 7 cut(s) 94, 166, 530, 556, 880, 1392, 2181
PspFI CCCAGC 1 cut(s) 151
PspGI CCWGG 7 cut(s) 94, 166, 530, 556, 880, 1392, 2181
PspLI CGTACG 1 cut(s) 765
PspN4I GGNNCC 2 cut(s) 616, 1318
PspPI GGNCC 2 cut(s) 1390, 2159
PstI CTGCAG 1 cut(s) 878
PstNI CAGNNNCTG 2 cut(s) 1641, 2256
PsyI GACNNNGTC 1 cut(s) 2191
PvuII CAGCTG 1 cut(s) 1378
RruI TCGCGA 1 cut(s) 1934
RsaI GTAC 8 cut(s) 52, 203, 494, 671, 767, 836, 1143, 1152
RsaNI GTAC 8 cut(s) 51, 202, 493, 670, 766, 835, 1142, 1151
RseI CAYNNNNRTG 5 cut(s) 1367, 1656, 1795, 2212, 2277
SacI GAGCTC 1 cut(s) 654
SalI GTCGAC 1 cut(s) 340
SaqAI TTAA 8 cut(s) 219, 311, 594, 1175, 1274, 1662, 1944, 2175
SatI GCNGC 7 cut(s) 476, 918, 1008, 1182, 1221, 1224, 1346
Sau3AI GATC 4 cut(s) 306, 353, 454, 967
Sau96I GGNCC 2 cut(s) 1390, 2159
ScaI AGTACT 2 cut(s) 836, 1152
SduI GDGCHC 3 cut(s) 118, 654, 1001
SexAI ACCWGGT 2 cut(s) 166, 556
SfaNI GCATC 4 cut(s) 406, 721, 904, 1332
SfcI CTRYAG 1 cut(s) 874
SfuI TTCGAA 2 cut(s) 953, 1098
SinI GGWCC 2 cut(s) 1390, 2159
SmiMI CAYNNNNRTG 5 cut(s) 1367, 1656, 1795, 2212, 2277
SmlI CTYRAG 4 cut(s) 310, 988, 1174, 2096
SmoI CTYRAG 4 cut(s) 310, 988, 1174, 2096
SnaBI TACGTA 1 cut(s) 190
SpeI ACTAGT 1 cut(s) 517
SphI GCATGC 1 cut(s) 1465
SsiI CCGC 5 cut(s) 138, 1220, 1465, 1927, 2132
SspMI CTAG 3 cut(s) 518, 830, 1578
SstI GAGCTC 1 cut(s) 654
StyI CCWWGG 1 cut(s) 72
TaaI ACNGT 8 cut(s) 21, 185, 226, 770, 1697, 1777, 1904, 2258
TaiI ACGT 5 cut(s) 32, 192, 543, 2195, 2295
TaqI TCGA 8 cut(s) 178, 291, 341, 371, 729, 792, 953, 1098
TatI WGTACW 4 cut(s) 492, 834, 1141, 1150
TauI GCSGC 1 cut(s) 1223
TfiI GAWTC 7 cut(s) 347, 425, 1100, 1244, 1470, 1685, 2086
Tru1I TTAA 8 cut(s) 219, 311, 594, 1175, 1274, 1662, 1944, 2175
Tru9I TTAA 8 cut(s) 219, 311, 594, 1175, 1274, 1662, 1944, 2175
TscAI CASTG 4 cut(s) 717, 878, 1374, 1658
TseI GCWGC 6 cut(s) 475, 917, 1007, 1181, 1223, 1345
TspDTI ATGAA 8 cut(s) 46, 225, 417, 597, 1641, 2022, 2196, 2300
TspGWI ACGGA 1 cut(s) 2151
TspRI CASTG 4 cut(s) 717, 878, 1374, 1658
Tth111I GACNNNGTC 1 cut(s) 2191
Vha464I CTTAAG 2 cut(s) 310, 1174
VpaK11BI GGWCC 2 cut(s) 1390, 2159
XapI RAATTY 2 cut(s) 12, 2091
XceI RCATGY 4 cut(s) 1051, 1465, 1516, 1852
XmiI GTMKAC 1 cut(s) 341
XspI CTAG 3 cut(s) 518, 830, 1578
ZrmI AGTACT 2 cut(s) 836, 1152
Zsp2I ATGCAT 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.