Rh2CG310900

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000916
Physical Location & Seq
Reverse (-)
39217743 .. 39220861
3119 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG310900.1

Sequence Viewer

Length: 2424 bp
ATGATTCATTTTGGTTCAGAGTGTGTGCTTGTTGTGTTATGTTGTTTCATCATCATCAGCTTCGGAGCTCATCTCTCAACGGCGTCTGACAAGCTCATCCCAGGCCGGTCACTTTCCGGCAACCAGACATTAACCTCTCCGGCAGGCAAATTTGAACTGGGTTTCTTCACTCGAGGTAACTCTAGCAATTACTACATAGGCATTTGGTACGCAAATCTGCCCGATCATACAGTTGTCTGGGTGGCTAATAGAAACCACCCTGTTTCTGATCCAGTTTCTTCAGAGTTTAGATTCCTTGAAAATGGAAACTTTACCTTGCTGGACCAGTCCAAATCTTCAATTTGGTCAACCCAGAATTCTATGTCTTCAGTGTCCAAATCCGCCACATATACAGCAAGGCTTCTTGACAATGGCAACTTTGTTATTCAAGATGCATCTGGTGTAATATGGCAGAGTTTTGATCACCCAACTGATACTTGGCTCCCAGGTGGTGCCAGGCTTGGTTACAACAAGCTTACCAATCAGAAACTAGCTCTCACTCCCTGGAAAAACCCACAAGACCCAGCCCCTGGAATTTTTTCCCTTGAGATTGAACCAAATGGGACAAGTTTTTGGTTGCTCTATAATGGCTCTCAACAGTATTGGAATAGTGGGCCTTGGAATGGCAAATCTTTTGCCCAAGTCCCTGAAATAGAGCGGAACGATCACATAACAAATGTTACTTTTGTTCCGGATGACAAGGGGAGCTATTTTTCTTATGATTCTGCAGACCCTGACACTTATATTAGGTACATGATTGACATCACTGGGCAGTTCAAGGTCTATATGTGGGGGAAGGACTTCATCCAGTGGAATTCATTTTGGTTGAGGCCAACCCAATTCTGTGAGGTTTATGGATTTTGTGGTGCTTCTAGCATCTGCCAGCAAAACGCTAGTCTGTGTGTTTGTATGGAGGGATATGAACCTAAAGTCCTAAAAGAGTGGGGATTAGGGGATCACACAGATGGCTGTGTCAGGAAAGCACCTTTAAAATGCATTGCTGGAGTAGATGATACTTTTAAGGTGGTATCAGATGTCCGCCTTCCTGTGAATCCAGAGACTTTAGCAGTTGATAACAGTGATGATTGCAGATTAGCGTGCTTAAGAAATTACTCATGTACAGCTTTTGCTTATGATAATGGGTGTCTTGTTTGGGGAGGGGATCTATTCAACGTCAAACAATTTACACCAGACGAGAAGGAGGGCAAGGACTTGCATCTGAAAATTCCAGCTTCTGAGAGAATAGAGGGGAAGAAAGAGAACAGGACTCTTTGGATCATCATCGGAGTACTTGGAGGTTTATTTATTGTTATTGCCATTGTTGTGGTCCTATTTTTGAAGCATCGATGTTTTGGGGAAAGGTTTGATACACTTGATGGTTCCTTGGTGATGTTCAGGTGTAGGGCTTTAAGAAAGGCAACAAACAACTTTTCGGAGAAACTTGGGGAAGGAGGCTTTGGTTCTGTTTTCAAAGGGACATTGCAGGGATCAATTGCCATTGCGGTGAAGAGACTCAACTGTCCCAAGCAAGAGGATAAGCAATTCCTTACAGAAGTGAGAACTATTGGAAAAGTCCAACACATTAATCTTATTCGTCTTTGTGGATTTTGTGCAGAAGCCTCAAAAAGATTCTTGGTTTACGAATACATGCCAAATGGGTCTCTAGAATCTGTTCTATTTAAAAACAGTTCCATTGTCTTAGATTGGAAAGCTAGGTACCAAATTGCAGTTGGGACTGCTAGAGGATTGGCCTACCTTCATGAGGAATGTAGAGAATGCATCATACACTGCGATATCAAACCTGAAAACATTCTCTTGGATGCAGAATATGTCCCAAAAGTAGCAGATTTTGGGCTAGCAAAGCTCATAGACAGAGACATTAGCCGGATAATAACAACCATGAAAGGAACCAGAGGCTATATTGCTCCAGAGTGGATTTCAGGAGAAGCTATAACATCGAAAGCTGATACCTTTAGCTATGGGATGCTGTTATTTGAACTTGTCTCGGGAAGGAGAAACAGAGATCTATTAGATGATGCTTTAGAAAATTACTTCCCAACCCGTGTTGCCAATGTGTTGAACAAAGAAGAAGATGTTGATGCCTTGTTGGATTACAGGTTAGAAGGTAATGCTGACAAAGAACAACTGAGTAGAGCATGCAAAGTCGCTTGTTGGTGCATTCAAGACGACGAGAAGGATAGGCCAACTATGGGGCAGGTTGTTCAACTTCTGGAGGGAGTTATAGACGCTGGAATTCCACCAATGCCAAAGTTCCTTGACCGCTTTTCTAAGAGTCTAGTTGAATCCATCAATTATCGGTACATCACTTCCAGCAGTTCTGATTCATGTCGTCATGAGTACTCAGATAGTATATATCAAGCTTAA

Protein Analysis

807

Amino Acids

90.67

Weight (kDa)

5.69

Isoelectric Point (pI)

31.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 77 - 183 3.5e-29 D-mannose binding lectin
S_locus_glycop PF00954 215 - 323 2.8e-22 S-locus glycoprotein domain
PAN_2 PF08276 348 - 405 7.1e-12 PAN-like domain
PK_Tyr_Ser-Thr PF07714 490 - 756 8e-44 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 491 - 755 3.3e-50 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1557
Acc36I ACCTGC 1 cut(s) 2245
Acc65I GGTACC 1 cut(s) 1755
AccB1I GGYRCC 2 cut(s) 491, 1755
AccB7I CCANNNNNTGG 1 cut(s) 569
AccBSI CCGCTC 1 cut(s) 697
AccIII TCCGGA 1 cut(s) 730
AciI CCGC 5 cut(s) 381, 697, 1078, 1541, 2320
AclWI GGATC 5 cut(s) 263, 1002, 1209, 1322, 1534
AcsI RAATTY 6 cut(s) 149, 355, 573, 853, 1263, 2292
AcuI CTGAAG 2 cut(s) 264, 351
AcyI GRCGYC 1 cut(s) 83
AfaI GTAC 7 cut(s) 209, 791, 1159, 1329, 1757, 2360, 2399
AfiI CCNNNNNNNGG 4 cut(s) 569, 662, 1801, 2249
AflII CTTAAG 1 cut(s) 1141
AjnI CCWGG 5 cut(s) 100, 484, 494, 542, 568
AjuI GAANNNNNNNTTGG 2 cut(s) 1479, 1511
AloI GAACNNNNNNTCC 2 cut(s) 954, 986
Alw21I GWGCWC 1 cut(s) 70
Alw26I GTCTC 5 cut(s) 1091, 1543, 1704, 1908, 2047
AlwI GGATC 5 cut(s) 263, 1002, 1209, 1322, 1534
AlwNI CAGNNNCTG 3 cut(s) 569, 773, 1274
Ama87I CYCGRG 2 cut(s) 171, 2044
Aor13HI TCCGGA 1 cut(s) 730
AoxI GGCC 5 cut(s) 103, 653, 869, 1788, 2240
ApoI RAATTY 6 cut(s) 149, 355, 573, 853, 1263, 2292
ArsI GACNNNNNNTTYG 2 cut(s) 919, 951
AseI ATTAAT 1 cut(s) 1623
Asp700I GAANNNNTTC 4 cut(s) 577, 839, 1710, 1848
Asp718I GGTACC 1 cut(s) 1755
AspS9I GGNCC 3 cut(s) 322, 653, 1366
AsuHPI GGTGA 3 cut(s) 455, 1438, 1555
AsuNHI GCTAGC 1 cut(s) 1894
AvaI CYCGRG 2 cut(s) 171, 2044
AvaII GGWCC 2 cut(s) 322, 1366
BanI GGYRCC 2 cut(s) 491, 1755
BanII GRGCYC 1 cut(s) 70
BbsI GAAGAC 1 cut(s) 357
Bbv12I GWGCWC 1 cut(s) 70
BccI CCATC 3 cut(s) 998, 1409, 2354
BceAI ACGGC 1 cut(s) 96
BciT130I CCWGG 5 cut(s) 102, 486, 496, 544, 570
BclI TGATCA 1 cut(s) 460
BcoDI GTCTC 5 cut(s) 1091, 1543, 1704, 1908, 2047
BfaI CTAG 9 cut(s) 183, 530, 912, 933, 1703, 1752, 1779, 1895, 2336
BfmI CTRYAG 1 cut(s) 765
BfrI CTTAAG 1 cut(s) 1141
BfuAI ACCTGC 1 cut(s) 2245
BglII AGATCT 1 cut(s) 2062
BmcAI AGTACT 2 cut(s) 1329, 2399
Bme1390I CCNGG 5 cut(s) 102, 486, 496, 544, 570
Bme18I GGWCC 2 cut(s) 322, 1366
BmeT110I CYCGRG 2 cut(s) 171, 2044
BmgT120I GGNCC 3 cut(s) 322, 653, 1366
BmiI GGNNCC 5 cut(s) 482, 493, 1420, 1757, 1948
BmrFI CCNGG 5 cut(s) 102, 486, 496, 544, 570
BmrI ACTGGG 2 cut(s) 167, 816
BmtI GCTAGC 1 cut(s) 1898
BmuI ACTGGG 2 cut(s) 167, 816
BpiI GAAGAC 1 cut(s) 357
BplI GAGNNNNNCTC 2 cut(s) 57, 89
BpmI CTGGAG 3 cut(s) 1062, 1950, 2291
BpuEI CTTGAG 1 cut(s) 605
Bsa29I ATCGAT 1 cut(s) 1384
BsaBI GATNNNNATC 2 cut(s) 800, 1319
BsaHI GRCGYC 1 cut(s) 83
BsaI GGTCTC 1 cut(s) 1704
BsaJI CCNNGG 6 cut(s) 100, 484, 542, 568, 656, 1422
BsaWI WCCGGW 1 cut(s) 730
Bsc4I CCNNNNNNNGG 4 cut(s) 569, 662, 1801, 2249
Bse118I RCCGGY 1 cut(s) 105
Bse1I ACTGG 5 cut(s) 162, 272, 325, 811, 847
Bse3DI GCAATG 3 cut(s) 1035, 1517, 1536
Bse8I GATNNNNATC 2 cut(s) 800, 1319
BseAI TCCGGA 1 cut(s) 730
BseBI CCWGG 5 cut(s) 102, 486, 496, 544, 570
BseCI ATCGAT 1 cut(s) 1384
BseDI CCNNGG 6 cut(s) 100, 484, 542, 568, 656, 1422
BseGI GGATG 5 cut(s) 96, 739, 843, 1864, 2028
BseJI GATNNNNATC 2 cut(s) 800, 1319
BseLI CCNNNNNNNGG 4 cut(s) 569, 662, 1801, 2249
BseMI GCAATG 3 cut(s) 1035, 1517, 1536
BseMII CTCAG 3 cut(s) 1266, 2177, 2415
BseNI ACTGG 5 cut(s) 162, 272, 325, 811, 847
BseYI CCCAGC 1 cut(s) 562
BsgI GTGCAG 1 cut(s) 1671
BshFI GGCC 5 cut(s) 105, 655, 871, 1790, 2242
BshNI GGYRCC 2 cut(s) 491, 1755
BshVI ATCGAT 1 cut(s) 1384
BsiHKAI GWGCWC 1 cut(s) 70
BsiHKCI CYCGRG 2 cut(s) 171, 2044
BsiSI CCGG 5 cut(s) 106, 117, 140, 731, 1924
BslFI GGGAC 6 cut(s) 616, 668, 1528, 1545, 1786, 1856
BslI CCNNNNNNNGG 4 cut(s) 569, 662, 1801, 2249
BsmAI GTCTC 5 cut(s) 1091, 1543, 1704, 1908, 2047
BsmFI GGGAC 6 cut(s) 616, 668, 1528, 1545, 1786, 1856
BsmI GAATGC 2 cut(s) 1820, 2217
BsnI GGCC 5 cut(s) 105, 655, 871, 1790, 2242
Bso31I GGTCTC 1 cut(s) 1704
BsoBI CYCGRG 2 cut(s) 171, 2044
Bsp1286I GDGCHC 1 cut(s) 70
Bsp13I TCCGGA 1 cut(s) 730
Bsp1407I TGTACA 1 cut(s) 1157
Bsp143I GATC 9 cut(s) 223, 268, 460, 703, 994, 1201, 1314, 1526, 2062
BspACI CCGC 5 cut(s) 381, 697, 1078, 1541, 2320
BspANI GGCC 5 cut(s) 105, 655, 871, 1790, 2242
BspCNI CTCAG 3 cut(s) 1267, 2178, 2414
BspDI ATCGAT 1 cut(s) 1384
BspEI TCCGGA 1 cut(s) 730
BspHI TCATGA 2 cut(s) 1798, 2392
BspLI GGNNCC 5 cut(s) 482, 493, 1420, 1757, 1948
BspMAI CTGCAG 1 cut(s) 769
BspMI ACCTGC 1 cut(s) 2245
BspOI GCTAGC 1 cut(s) 1898
BspPI GGATC 5 cut(s) 263, 1002, 1209, 1322, 1534
BspT107I GGYRCC 2 cut(s) 491, 1755
BspTI CTTAAG 1 cut(s) 1141
BspTNI GGTCTC 1 cut(s) 1704
BsrBI CCGCTC 1 cut(s) 697
BsrDI GCAATG 3 cut(s) 1035, 1517, 1536
BsrFI RCCGGY 1 cut(s) 105
BsrGI TGTACA 1 cut(s) 1157
BsrI ACTGG 5 cut(s) 162, 272, 325, 811, 847
BssAI RCCGGY 1 cut(s) 105
BssECI CCNNGG 6 cut(s) 100, 484, 542, 568, 656, 1422
BssMI GATC 9 cut(s) 223, 268, 460, 703, 994, 1201, 1314, 1526, 2062
BssNI GRCGYC 1 cut(s) 83
BssT1I CCWWGG 2 cut(s) 656, 1422
Bst2UI CCWGG 5 cut(s) 102, 486, 496, 544, 570
Bst4CI ACNGT 5 cut(s) 232, 639, 1118, 1559, 1727
Bst6I CTCTTC 1 cut(s) 1541
BstACI GRCGYC 1 cut(s) 83
BstAFI CTTAAG 1 cut(s) 1141
BstAUI TGTACA 1 cut(s) 1157
BstC8I GCNNGC 5 cut(s) 145, 923, 1138, 1896, 2197
BstDEI CTNAG 5 cut(s) 1275, 1738, 2186, 2328, 2401
BstENI CCTNNNNNAGG 1 cut(s) 1799
BstF5I GGATG 5 cut(s) 96, 739, 843, 1864, 2028
BstKTI GATC 9 cut(s) 226, 271, 463, 706, 997, 1204, 1317, 1529, 2065
BstMAI GTCTC 5 cut(s) 1091, 1543, 1704, 1908, 2047
BstMBI GATC 9 cut(s) 223, 268, 460, 703, 994, 1201, 1314, 1526, 2062
BstMWI GCNNNNNNNGC 1 cut(s) 1900
BstNI CCWGG 5 cut(s) 102, 486, 496, 544, 570
BstNSI RCATGY 2 cut(s) 1690, 2199
BstSCI CCNGG 5 cut(s) 100, 484, 494, 542, 568
BstSFI CTRYAG 1 cut(s) 765
BstV2I GAAGAC 1 cut(s) 357
BstX2I RGATCY 2 cut(s) 1201, 2062
BstXI CCANNNNNNTGG 1 cut(s) 1363
BstYI RGATCY 2 cut(s) 1201, 2062
Bsu15I ATCGAT 1 cut(s) 1384
BsuRI GGCC 5 cut(s) 105, 655, 871, 1790, 2242
BsuTUI ATCGAT 1 cut(s) 1384
BtsCI GGATG 5 cut(s) 96, 739, 843, 1864, 2028
BtsI GCAGTG 1 cut(s) 1825
BtsIMutI CAGTG 5 cut(s) 375, 804, 854, 1123, 1825
BveI ACCTGC 1 cut(s) 2245
Cac8I GCNNGC 5 cut(s) 145, 923, 1138, 1896, 2197
CaiI CAGNNNCTG 3 cut(s) 569, 773, 1274
CciI TCATGA 2 cut(s) 1798, 2392
Cfr10I RCCGGY 1 cut(s) 105
Cfr13I GGNCC 3 cut(s) 322, 653, 1366
ClaI ATCGAT 1 cut(s) 1384
CseI GACGC 2 cut(s) 72, 2294
Csp6I GTAC 7 cut(s) 208, 790, 1158, 1328, 1756, 2359, 2398
CviAII CATG 8 cut(s) 793, 1155, 1687, 1799, 1939, 2196, 2385, 2393
CviQI GTAC 7 cut(s) 208, 790, 1158, 1328, 1756, 2359, 2398
DdeI CTNAG 5 cut(s) 1275, 1738, 2186, 2328, 2401
DpnI GATC 9 cut(s) 225, 270, 462, 705, 996, 1203, 1316, 1528, 2064
DpnII GATC 9 cut(s) 223, 268, 460, 703, 994, 1201, 1314, 1526, 2062
DraI TTTAAA 2 cut(s) 1029, 1720
DrdI GACNNNNNNGTC 1 cut(s) 1557
DseDI GACNNNNNNGTC 1 cut(s) 1557
Eam1104I CTCTTC 1 cut(s) 1541
EarI CTCTTC 1 cut(s) 1541
EciI GGCGGA 2 cut(s) 370, 1067
Ecl136II GAGCTC 1 cut(s) 68
Eco130I CCWWGG 2 cut(s) 656, 1422
Eco24I GRGCYC 1 cut(s) 70
Eco31I GGTCTC 1 cut(s) 1704
Eco32I GATATC 1 cut(s) 1834
Eco47I GGWCC 2 cut(s) 322, 1366
Eco53kI GAGCTC 1 cut(s) 68
Eco57I CTGAAG 2 cut(s) 264, 351
Eco88I CYCGRG 2 cut(s) 171, 2044
EcoICRI GAGCTC 1 cut(s) 68
EcoNI CCTNNNNNAGG 1 cut(s) 1799
EcoRI GAATTC 3 cut(s) 355, 853, 2292
EcoRII CCWGG 5 cut(s) 100, 484, 494, 542, 568
EcoRV GATATC 1 cut(s) 1834
EcoT14I CCWWGG 2 cut(s) 656, 1422
EcoT22I ATGCAT 3 cut(s) 436, 1037, 1820
EcoT38I GRGCYC 1 cut(s) 70
ErhI CCWWGG 2 cut(s) 656, 1422
FaeI CATG 8 cut(s) 796, 1158, 1690, 1802, 1942, 2199, 2388, 2396
FaqI GGGAC 6 cut(s) 616, 668, 1528, 1545, 1786, 1856
FatI CATG 8 cut(s) 792, 1154, 1686, 1798, 1938, 2195, 2384, 2392
FbaI TGATCA 1 cut(s) 460
FokI GGATG 5 cut(s) 83, 746, 830, 1871, 2035
FriOI GRGCYC 1 cut(s) 70
FspBI CTAG 9 cut(s) 183, 530, 912, 933, 1703, 1752, 1779, 1895, 2336
GsaI CCCAGC 1 cut(s) 566
GsuI CTGGAG 3 cut(s) 1062, 1950, 2291
HaeIII GGCC 5 cut(s) 105, 655, 871, 1790, 2242
HapII CCGG 5 cut(s) 106, 117, 140, 731, 1924
HgaI GACGC 2 cut(s) 72, 2294
Hin1I GRCGYC 1 cut(s) 83
Hin1II CATG 8 cut(s) 796, 1158, 1690, 1802, 1942, 2199, 2388, 2396
HincII GTYRAC 1 cut(s) 348
HindII GTYRAC 1 cut(s) 348
HindIII AAGCTT 2 cut(s) 512, 2418
HpaII CCGG 5 cut(s) 106, 117, 140, 731, 1924
HphI GGTGA 3 cut(s) 455, 1438, 1555
Hpy166II GTNNAC 2 cut(s) 348, 1678
Hpy8I GTNNAC 2 cut(s) 348, 1678
Hpy99I CGWCG 1 cut(s) 2231
HpyAV CCTTC 8 cut(s) 829, 1091, 1231, 1481, 1805, 2043, 2156, 2227
HpyCH4III ACNGT 5 cut(s) 232, 639, 1118, 1559, 1727
HpyCH4IV ACGT 1 cut(s) 1212
HpyF10VI GCNNNNNNNGC 1 cut(s) 1900
HpyF3I CTNAG 5 cut(s) 1275, 1738, 2186, 2328, 2401
HpySE526I ACGT 1 cut(s) 1212
Hsp92I GRCGYC 1 cut(s) 83
Hsp92II CATG 8 cut(s) 796, 1158, 1690, 1802, 1942, 2199, 2388, 2396
Kpn2I TCCGGA 1 cut(s) 730
KpnI GGTACC 1 cut(s) 1759
Ksp22I TGATCA 1 cut(s) 460
Kzo9I GATC 9 cut(s) 223, 268, 460, 703, 994, 1201, 1314, 1526, 2062
LmnI GCTCC 4 cut(s) 65, 486, 744, 1969
MaeI CTAG 9 cut(s) 183, 530, 912, 933, 1703, 1752, 1779, 1895, 2336
MaeII ACGT 1 cut(s) 1212
MaeIII GTNAC 4 cut(s) 108, 176, 503, 718
MalI GATC 9 cut(s) 225, 270, 462, 705, 996, 1203, 1316, 1528, 2064
MbiI CCGCTC 1 cut(s) 697
MboI GATC 9 cut(s) 223, 268, 460, 703, 994, 1201, 1314, 1526, 2062
MboII GAAGA 8 cut(s) 157, 270, 327, 357, 1303, 1558, 2138, 2141
MfeI CAATTG 1 cut(s) 1530
MflI RGATCY 2 cut(s) 1201, 2062
MhlI GDGCHC 1 cut(s) 70
MlyI GAGTC 3 cut(s) 1300, 1545, 2341
MmeI TCCRAC 2 cut(s) 1639, 2127
Mph1103I ATGCAT 3 cut(s) 436, 1037, 1820
MroI TCCGGA 1 cut(s) 730
MroXI GAANNNNTTC 4 cut(s) 577, 839, 1710, 1848
MseI TTAA 8 cut(s) 131, 1028, 1059, 1142, 1448, 1623, 1719, 2422
MslI CAYNNNNRTG 3 cut(s) 1002, 1361, 1541
MspCI CTTAAG 1 cut(s) 1141
MspI CCGG 5 cut(s) 106, 117, 140, 731, 1924
MspR9I CCNGG 5 cut(s) 102, 486, 496, 544, 570
MunI CAATTG 1 cut(s) 1530
Mva1269I GAATGC 2 cut(s) 1820, 2217
MvaI CCWGG 5 cut(s) 102, 486, 496, 544, 570
MwoI GCNNNNNNNGC 1 cut(s) 1900
NdeII GATC 9 cut(s) 223, 268, 460, 703, 994, 1201, 1314, 1526, 2062
NheI GCTAGC 1 cut(s) 1894
NlaIII CATG 8 cut(s) 796, 1158, 1690, 1802, 1942, 2199, 2388, 2396
NlaIV GGNNCC 5 cut(s) 482, 493, 1420, 1757, 1948
NmuCI GTSAC 1 cut(s) 108
NsiI ATGCAT 3 cut(s) 436, 1037, 1820
NspI RCATGY 2 cut(s) 1690, 2199
PaeI GCATGC 1 cut(s) 2199
PaeR7I CTCGAG 1 cut(s) 171
PagI TCATGA 2 cut(s) 1798, 2392
PctI GAATGC 2 cut(s) 1820, 2217
PdmI GAANNNNTTC 4 cut(s) 577, 839, 1710, 1848
PfeI GAWTC 8 cut(s) 4, 291, 761, 1090, 1668, 1706, 2342, 2381
PflMI CCANNNNNTGG 1 cut(s) 569
PleI GAGTC 3 cut(s) 1300, 1545, 2340
PpsI GAGTC 3 cut(s) 1300, 1545, 2340
PshBI ATTAAT 1 cut(s) 1623
Psp124BI GAGCTC 1 cut(s) 70
Psp6I CCWGG 5 cut(s) 100, 484, 494, 542, 568
PspFI CCCAGC 1 cut(s) 562
PspGI CCWGG 5 cut(s) 100, 484, 494, 542, 568
PspN4I GGNNCC 5 cut(s) 482, 493, 1420, 1757, 1948
PspPI GGNCC 3 cut(s) 322, 653, 1366
PspXI VCTCGAGB 1 cut(s) 171
PstI CTGCAG 1 cut(s) 769
PstNI CAGNNNCTG 3 cut(s) 569, 773, 1274
PsuI RGATCY 2 cut(s) 1201, 2062
RsaI GTAC 7 cut(s) 209, 791, 1159, 1329, 1757, 2360, 2399
RsaNI GTAC 7 cut(s) 208, 790, 1158, 1328, 1756, 2359, 2398
RseI CAYNNNNRTG 3 cut(s) 1002, 1361, 1541
SacI GAGCTC 1 cut(s) 70
SaqAI TTAA 8 cut(s) 131, 1028, 1059, 1142, 1448, 1623, 1719, 2422
Sau3AI GATC 9 cut(s) 223, 268, 460, 703, 994, 1201, 1314, 1526, 2062
Sau96I GGNCC 3 cut(s) 322, 653, 1366
ScaI AGTACT 2 cut(s) 1329, 2399
SchI GAGTC 3 cut(s) 1300, 1545, 2341
ScrFI CCNGG 5 cut(s) 102, 486, 496, 544, 570
SduI GDGCHC 1 cut(s) 70
SfcI CTRYAG 1 cut(s) 765
Sfr274I CTCGAG 1 cut(s) 171
SinI GGWCC 2 cut(s) 322, 1366
SlaI CTCGAG 1 cut(s) 171
SmiMI CAYNNNNRTG 3 cut(s) 1002, 1361, 1541
SmlI CTYRAG 3 cut(s) 171, 584, 1141
SmoI CTYRAG 3 cut(s) 171, 584, 1141
SphI GCATGC 1 cut(s) 2199
SsiI CCGC 5 cut(s) 381, 697, 1078, 1541, 2320
SspMI CTAG 9 cut(s) 183, 530, 912, 933, 1703, 1752, 1779, 1895, 2336
SstI GAGCTC 1 cut(s) 70
StyD4I CCNGG 5 cut(s) 100, 484, 494, 542, 568
StyI CCWWGG 2 cut(s) 656, 1422
TaaI ACNGT 5 cut(s) 232, 639, 1118, 1559, 1727
TaiI ACGT 1 cut(s) 1215
TaqI TCGA 3 cut(s) 172, 1384, 1997
TatI WGTACW 3 cut(s) 1157, 1327, 2397
TfiI GAWTC 8 cut(s) 4, 291, 761, 1090, 1668, 1706, 2342, 2381
Tru1I TTAA 8 cut(s) 131, 1028, 1059, 1142, 1448, 1623, 1719, 2422
Tru9I TTAA 8 cut(s) 131, 1028, 1059, 1142, 1448, 1623, 1719, 2422
TscAI CASTG 5 cut(s) 375, 811, 854, 1123, 1832
TseFI GTSAC 1 cut(s) 108
Tsp45I GTSAC 1 cut(s) 108
TspDTI ATGAA 7 cut(s) 37, 832, 846, 975, 1787, 1955, 2373
TspRI CASTG 5 cut(s) 375, 811, 854, 1123, 1832
Van91I CCANNNNNTGG 1 cut(s) 569
Vha464I CTTAAG 1 cut(s) 1141
VpaK11BI GGWCC 2 cut(s) 322, 1366
VspI ATTAAT 1 cut(s) 1623
XagI CCTNNNNNAGG 1 cut(s) 1799
XapI RAATTY 6 cut(s) 149, 355, 573, 853, 1263, 2292
XbaI TCTAGA 1 cut(s) 1702
XceI RCATGY 2 cut(s) 1690, 2199
XcmI CCANNNNNNNNNTGG 2 cut(s) 474, 1766
XhoI CTCGAG 1 cut(s) 171
XmnI GAANNNNTTC 4 cut(s) 577, 839, 1710, 1848
XspI CTAG 9 cut(s) 183, 530, 912, 933, 1703, 1752, 1779, 1895, 2336
ZrmI AGTACT 2 cut(s) 1329, 2399
Zsp2I ATGCAT 3 cut(s) 436, 1037, 1820
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.