Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0000916

Physical Location & Seq

Genomic Coordinates Forward (+)

28760353 .. 28762820

Gene Structure 2468 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1238400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2253 bp

ATGATGAATAGCAGCAGGAACAAACTCAGTTACATGTCATCAGTTGTGGTTCTTGTGTTATGCTTGAACTTGAAAGCCGCCCATGTGTCGACGGCGTCTGGCAACATCATCTCCCAAGGCCAATCTATTTCCGGCAACCAAACAATCAGCTCATCACCACGCGGCATATTCGAGCTTGGTTTCTTCACTCCAGGTAACTCCCACAACCACTACATAGGCATCTGGTACAAAAAACTACAAAAAACCGTTGTTTGGGTGGCCAACAGAAACCACCCTGTTTCCGATCCATTTTCTTCCTCCCTTCAACTCTTCCCAAACGGAACCTTAATACTGCTCGACCAATCCAAATCTTCCATTTGGTCAACAACTCGTGATCTCTCAGTTGCTTCTAATTCTACCAGTGAAGTAGCAGCAGTGCTTCTTGACAATGGCAACTTTGTCATAACAGATGCTTTGAATTCGTCTGCTGTCATATGGCAGAGTTTCGATCACCCGACCGACACTTGGCTGCCGGGCGGGAAGCTTGGATACAACAAGCTGACCAATGAGAAACTAACCCTCACTCCCTGGAGAAGCTCACAAAACCCAGCACCTGGGATTTTCTCTCTAGAGATCGAACGAAACGGAACAAGTTTTCTATTGATGTATAATGGGACTAAAATGTATTGGACCAGCGGGCCTTGGACAGGCAAGATTTTTAAGTATGTTCCGGAAATTCAGTTGAATTATTTGGTCACGAATATCAGCTATAAGTCCGATGAATTTGGGAGTTATGTTTCTTATGATGCTGTTTTCCCCGACATCTTTGTCCGGTACATGCTTGATATCTCCGGGCAGTTCAGGGCCTACAAGTGGGGGAAGGACTATAATCAGTGGACTTCATTTTGGTTGCGACCCTCCGAACATTGCGAGGTATATGGATTCTGCGGTAAGAACATCGATGAGTGTAGATCGGCGTGCTTGAGTGATTGCTCGTGTACAGCGTTTGCTTATGACAGTGGGTGTTTAGTTTGGAAAGGGGATTTGTTCAATGTAAAACAACTTAAATCTGATGAGACAGTCGGCAAAGAGTTGTATCTTCGGGTTGCAGCATCAGAGGAACCAAAGGCGAAGAGAGAGAATAAAACCTTTTGGATTGTCATTGGAGTACTTGGAGGTTTGTGTGGTGTTTTACTGGTTATTGTGGTAATTGCCAAGAATGAATGTTCCGGTTCTGGGGAAAGGTCTGAGGCAGATGAAGGCTCTTTGACAATGTTCAAGTACCGGGTTCTAAGAAACGCAACCAAGAACTTTACTGAAAAACTTGGGGAAGGAGGCTTTGGTTCTGTTTTCAGAGGGACACTGAAGAGTTATACCGCCATCGCAGTGAAGAGGCTTAACTGTCCAAAGCAAGCAGATAAGCAATTTCTTACAGAAGTGAGGACTCTTGGAAAGGTCCAGCACATTAATCTTGTTCGTCTCCGCGGATTTTGTGCAGAAACTTCAAAAAGATTCTTGGTTTATGATTACATGCCAAATGGTTCTCTAGAATCTCTTTTGTTCCAGAAGAGTCCGATTGTCTTGAATTGGAAAACTAGATATCACATTGCAGTTGGGACTGCAAGAGGACTTGCATATCTTCATCATAGTTGCAGAGAATGCATCATACACTGTGACATCAAACCTGAAAATATTCTGTTGGATGCAGAATATGCCCCCAAAATAGCTGATTTTGGTCTTGCAAAGCTCGTGAGCAGAGACTTCAGCCGGGTAATTACAACCATGCAAGGGACAAGAGGGTATATTGCTCCGGAATGGATATCAGGAGAAGCTATCACAACAAAAGCCGATGTCTTTAGCTACGGAATGTTGTGTTTTGAGATCATATCAGGAAGGAGAAACAGAGATCTTTTGGATGATGGGTTGCAAAACTACTTCCCCACTCGTGTTGCAAATGTATTAACGAAAGGAGAAGATGTCGATACCTTGTTGGATTGCAGGTTAGAAGGTAATGCAAACAAAGAAGAAGTGATGAGAGCATGCAAAGTAGCATGTTGGTGCATTCAAGATGACGAGAACGATAGGCCGACAATGGGGCAAGTTGTTCAACTTCTGGAAGGAGTCATAGATCTCGGCATACCTCCTATCCCACAGTTCCTCGACCGGTTTTCAAAGAGTCTAGGTGAGTCCATACATTACCATAACATTTCTTCCAGTACTGCTTCGGATTCAAGGCGTGGCTGCTCGAGTAGTATTACTATATCTGGAGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

B_lectin PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

751

Amino Acids

83.67

Weight (kDa)

8.32

Isoelectric Point (pI)

43.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	82 - 191	5.6e-33	D-mannose binding lectin
S_locus_glycop	PF00954	223 - 327	1e-15	S-locus glycoprotein domain
PAN_2	PF08276	311 - 345	2.4e-10	PAN-like domain
PK_Tyr_Ser-Thr	PF07714	431 - 697	2.7e-44	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	432 - 696	3.8e-49	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000916)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g26420 FvH4_6g26420 FvH4_6g26450 FvH4_6g26451
malus_domestica	MD09G1116700.v1.1 MD09G1233200.v1.1 MD12G1033500.v1.1 MD14G1034400.v1.1 MD14G1034600.v1.1 MD14G1034700.v1.1 MD14G1034800.v1.1 MD14G1034900.v1.1 MD14G1035200.v1.1 MD17G1090300.v1.1 MD17G1108200.v1.1 MD17G1237700.v1.1 MD17G1237800.v1.1 MD17G1238000.v1.1 MD17G1238400.v1.1
prunus_persica	Prupe.3G131100_v2.0.a1 Prupe.3G141400_v2.0.a1
pyrus_communis	pycom09g04120 pycom12g03080 pycom14g03020 pycom14g03080 pycom14g03090 pycom17g24250
rosa_chinensis	RchiOBHm_Chr2g0127791 RchiOBHm_Chr2g0127811 RchiOBHm_Chr2g0127821 RchiOBHm_Chr2g0128001
rosa_laevigata	RLG00000002113 RLG00000018931 RLG00000018932 RLG00000018933 RLG00000018934 RLG00000023426
rosa_multiflora	Rmu_sc0000516.1_g000026 Rmu_sc0000516.1_g000045 Rmu_ssc0000093.1_g000010 Rmu_ssc0000093.1_g000016 Rmu_ssc0000093.1_g000017 Rmu_ssc0000093.1_g000024
rosa_roxburghii	Rroxscaffold_2G00116790 Rroxscaffold_2G00116800 Rroxscaffold_2G00116810 Rroxscaffold_2G00116840
rosa_rugosa	Rorug02G0270500 Rorug02G0270600 Rorug02G0270800 Rorug02G0270900 Rorug02G0271000
rosa_samantha	Rh2AG323100 Rh2BG332100 Rh2BG332200 Rh2BG332300 Rh2CG310900 Rh2CG311000 Rh2DG350400 Rh2DG350500 Rh2DG350600 Rh2DG350700
rosa_wichuraiana	Rw2G026190 Rw2G026210 Rw2G026230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	806
Acc36I	ACCTGC	1 cut(s)	1968
AccB7I	CCANNNNNTGG	1 cut(s)	593
AccI	GTMKAC	1 cut(s)	89
AccII	CGCG	2 cut(s)	162, 1464
AccIII	TCCGGA	2 cut(s)	709, 1789
AciI	CCGC	8 cut(s)	78, 162, 516, 675, 927, 1356, 1462, 1464
AclWI	GGATC	1 cut(s)	278
AcoI	YGGCCR	1 cut(s)	258
AcsI	RAATTY	3 cut(s)	457, 714, 761
AcuI	CTGAAG	2 cut(s)	1364, 1726
AcyI	GRCGYC	1 cut(s)	95
AfaI	GTAC	6 cut(s)	227, 815, 979, 1149, 1262, 2197
AfiI	CCNNNNNNNGG	8 cut(s)	252, 504, 593, 686, 852, 1215, 1767, 2072
AflIII	ACRYGT	1 cut(s)	33
AgeI	ACCGGT	1 cut(s)	2142
AjnI	CCWGG	3 cut(s)	190, 566, 592
AjuI	GAANNNNNNNTTGG	2 cut(s)	1302, 1334
Alw26I	GTCTC	3 cut(s)	1049, 1463, 1731
AlwI	GGATC	1 cut(s)	278
AlwNI	CAGNNNCTG	1 cut(s)	593
Ama87I	CYCGRG	1 cut(s)	2224
Aor13HI	TCCGGA	2 cut(s)	709, 1789
AoxI	GGCC	5 cut(s)	118, 258, 677, 843, 2063
ApeKI	GCWGC	5 cut(s)	12, 410, 508, 1088, 2220
ApoI	RAATTY	3 cut(s)	457, 714, 761
AseI	ATTAAT	1 cut(s)	1446
AsiGI	ACCGGT	1 cut(s)	2142
Asp700I	GAANNNNTTC	1 cut(s)	1671
AspS9I	GGNCC	4 cut(s)	669, 677, 843, 1435
AsuC2I	CCSGG	4 cut(s)	513, 832, 1265, 1748
AsuHPI	GGTGA	3 cut(s)	147, 482, 2174
AvaI	CYCGRG	1 cut(s)	2224
AvaII	GGWCC	2 cut(s)	669, 1435
BalI	TGGCCA	1 cut(s)	260
BarI	GAAGNNNNNNTAC	2 cut(s)	512, 544
BauI	CACGAG	4 cut(s)	369, 973, 1727, 1923
BbvI	GCAGC	5 cut(s)	24, 422, 495, 1100, 2207
BccI	CCATC	2 cut(s)	1367, 1892
BceAI	ACGGC	1 cut(s)	108
BciT130I	CCWGG	3 cut(s)	192, 568, 594
BciVI	GTATCC	1 cut(s)	521
BcnI	CCSGG	4 cut(s)	513, 832, 1265, 1748
BcoDI	GTCTC	3 cut(s)	1049, 1463, 1731
BfaI	CTAG	4 cut(s)	608, 1526, 1575, 2159
BfuAI	ACCTGC	1 cut(s)	1968
BfuI	GTATCC	1 cut(s)	521
BglII	AGATCT	2 cut(s)	1885, 2107
BisI	GCNGC	7 cut(s)	13, 78, 163, 411, 509, 1089, 2221
BlsI	GCNGC	7 cut(s)	14, 79, 164, 412, 510, 1090, 2222
BmcAI	AGTACT	2 cut(s)	1149, 2197
Bme1390I	CCNGG	7 cut(s)	192, 513, 568, 594, 832, 1265, 1748
Bme18I	GGWCC	2 cut(s)	669, 1435
BmeT110I	CYCGRG	1 cut(s)	2224
BmgT120I	GGNCC	4 cut(s)	669, 677, 843, 1435
BmiI	GGNNCC	2 cut(s)	322, 1101
BmrFI	CCNGG	7 cut(s)	192, 513, 568, 594, 832, 1265, 1748
BmsI	GCATC	6 cut(s)	228, 439, 775, 1100, 1650, 1672
BpmI	CTGGAG	2 cut(s)	174, 589
BpuEI	CTTGAG	1 cut(s)	982
BpuMI	CCSGG	4 cut(s)	513, 832, 1265, 1748
Bsa29I	ATCGAT	1 cut(s)	939
BsaHI	GRCGYC	1 cut(s)	95
BsaJI	CCNNGG	5 cut(s)	115, 566, 593, 680, 1462
BsaWI	WCCGGW	5 cut(s)	709, 810, 1208, 1789, 2142
BsaXI	ACNNNNNCTCC	6 cut(s)	95, 125, 547, 577, 1941, 1971
Bsc4I	CCNNNNNNNGG	8 cut(s)	252, 504, 593, 686, 852, 1215, 1767, 2072
Bse118I	RCCGGY	1 cut(s)	2142
Bse1I	ACTGG	3 cut(s)	399, 1179, 2193
Bse3DI	GCAATG	2 cut(s)	904, 1584
BseAI	TCCGGA	2 cut(s)	709, 1789
BseBI	CCWGG	3 cut(s)	192, 568, 594
BseCI	ATCGAT	1 cut(s)	939
BseDI	CCNNGG	5 cut(s)	115, 566, 593, 680, 1462
BseGI	GGATG	2 cut(s)	1687, 1900
BseLI	CCNNNNNNNGG	8 cut(s)	252, 504, 593, 686, 852, 1215, 1767, 2072
BseMI	GCAATG	2 cut(s)	904, 1584
BseMII	CTCAG	3 cut(s)	40, 393, 1218
BseNI	ACTGG	3 cut(s)	399, 1179, 2193
BseXI	GCAGC	5 cut(s)	24, 422, 495, 1100, 2207
BseYI	CCCAGC	1 cut(s)	586
BsgI	GTGCAG	1 cut(s)	1494
Bsh1236I	CGCG	2 cut(s)	162, 1464
Bsh1285I	CGRYCG	2 cut(s)	498, 2143
BshFI	GGCC	5 cut(s)	120, 260, 679, 845, 2065
BshTI	ACCGGT	1 cut(s)	2142
BshVI	ATCGAT	1 cut(s)	939
BsiEI	CGRYCG	2 cut(s)	498, 2143
BsiHKCI	CYCGRG	1 cut(s)	2224
BslFI	GGGAC	4 cut(s)	667, 1351, 1609, 1783
BslI	CCNNNNNNNGG	8 cut(s)	252, 504, 593, 686, 852, 1215, 1767, 2072
BsmAI	GTCTC	3 cut(s)	1049, 1463, 1731
BsmBI	CGTCTC	1 cut(s)	1463
BsmFI	GGGAC	4 cut(s)	667, 1351, 1609, 1783
BsmI	GAATGC	2 cut(s)	1643, 2040
BsnI	GGCC	5 cut(s)	120, 260, 679, 845, 2065
BsoBI	CYCGRG	1 cut(s)	2224
Bsp13I	TCCGGA	2 cut(s)	709, 1789
Bsp1407I	TGTACA	1 cut(s)	977
Bsp143I	GATC	8 cut(s)	283, 373, 487, 612, 950, 1860, 1885, 2107
BspACI	CCGC	8 cut(s)	78, 162, 516, 675, 927, 1356, 1462, 1464
BspANI	GGCC	5 cut(s)	120, 260, 679, 845, 2065
BspCNI	CTCAG	3 cut(s)	39, 392, 1219
BspDI	ATCGAT	1 cut(s)	939
BspEI	TCCGGA	2 cut(s)	709, 1789
BspFNI	CGCG	2 cut(s)	162, 1464
BspLI	GGNNCC	2 cut(s)	322, 1101
BspMI	ACCTGC	1 cut(s)	1968
BspPI	GGATC	1 cut(s)	278
BsrDI	GCAATG	2 cut(s)	904, 1584
BsrFI	RCCGGY	1 cut(s)	2142
BsrGI	TGTACA	1 cut(s)	977
BsrI	ACTGG	3 cut(s)	399, 1179, 2193
BssAI	RCCGGY	1 cut(s)	2142
BssECI	CCNNGG	5 cut(s)	115, 566, 593, 680, 1462
BssMI	GATC	8 cut(s)	283, 373, 487, 612, 950, 1860, 1885, 2107
BssNI	GRCGYC	1 cut(s)	95
BssSI	CACGAG	4 cut(s)	369, 973, 1727, 1923
BssT1I	CCWWGG	2 cut(s)	115, 680
Bst2BI	CACGAG	4 cut(s)	369, 973, 1727, 1923
Bst2UI	CCWGG	3 cut(s)	192, 568, 594
Bst4CI	ACNGT	6 cut(s)	247, 998, 1060, 1382, 1652, 2133
Bst6I	CTCTTC	5 cut(s)	314, 1106, 1340, 1364, 1541
BstACI	GRCGYC	1 cut(s)	95
BstAPI	GCANNNNNTGC	2 cut(s)	1638, 1691
BstAUI	TGTACA	1 cut(s)	977
BstC8I	GCNNGC	4 cut(s)	677, 958, 1392, 2020
BstDEI	CTNAG	4 cut(s)	26, 379, 1227, 1271
BstDSI	CCRYGG	1 cut(s)	1462
BstENI	CCTNNNNNAGG	1 cut(s)	684
BstF5I	GGATG	2 cut(s)	1687, 1900
BstFNI	CGCG	2 cut(s)	162, 1464
BstKTI	GATC	8 cut(s)	286, 376, 490, 615, 953, 1863, 1888, 2110
BstMAI	GTCTC	3 cut(s)	1049, 1463, 1731
BstMBI	GATC	8 cut(s)	283, 373, 487, 612, 950, 1860, 1885, 2107
BstMCI	CGRYCG	2 cut(s)	498, 2143
BstMWI	GCNNNNNNNGC	2 cut(s)	1638, 1691
BstNI	CCWGG	3 cut(s)	192, 568, 594
BstNSI	RCATGY	5 cut(s)	37, 820, 1513, 2022, 2034
BstSCI	CCNGG	7 cut(s)	190, 511, 566, 592, 830, 1263, 1746
BstUI	CGCG	2 cut(s)	162, 1464
BstV1I	GCAGC	5 cut(s)	24, 422, 495, 1100, 2207
BstX2I	RGATCY	2 cut(s)	1885, 2107
BstYI	RGATCY	2 cut(s)	1885, 2107
Bsu15I	ATCGAT	1 cut(s)	939
BsuI	GTATCC	1 cut(s)	521
BsuRI	GGCC	5 cut(s)	120, 260, 679, 845, 2065
BsuTUI	ATCGAT	1 cut(s)	939
BtgI	CCRYGG	1 cut(s)	1462
BtgZI	GCGATG	1 cut(s)	1345
BtsCI	GGATG	2 cut(s)	1687, 1900
BtsI	GCAGTG	2 cut(s)	420, 1371
BtsIMutI	CAGTG	7 cut(s)	406, 420, 878, 1003, 1340, 1371, 1648
BveI	ACCTGC	1 cut(s)	1968
Cac8I	GCNNGC	4 cut(s)	677, 958, 1392, 2020
CaiI	CAGNNNCTG	1 cut(s)	593
Cfr10I	RCCGGY	1 cut(s)	2142
Cfr13I	GGNCC	4 cut(s)	669, 677, 843, 1435
Cfr42I	CCGCGG	1 cut(s)	1465
ClaI	ATCGAT	1 cut(s)	939
CseI	GACGC	1 cut(s)	84
Csp6I	GTAC	6 cut(s)	226, 814, 978, 1148, 1261, 2196
CspAI	ACCGGT	1 cut(s)	2142
CspCI	CAANNNNNGTGG	2 cut(s)	1909, 1944
CviAII	CATG	7 cut(s)	34, 83, 817, 1510, 1762, 2019, 2031
CviQI	GTAC	6 cut(s)	226, 814, 978, 1148, 1261, 2196
DdeI	CTNAG	4 cut(s)	26, 379, 1227, 1271
DpnI	GATC	8 cut(s)	285, 375, 489, 614, 952, 1862, 1887, 2109
DpnII	GATC	8 cut(s)	283, 373, 487, 612, 950, 1860, 1885, 2107
DrdI	GACNNNNNNGTC	1 cut(s)	806
DseDI	GACNNNNNNGTC	1 cut(s)	806
EaeI	YGGCCR	1 cut(s)	258
Eam1104I	CTCTTC	5 cut(s)	314, 1106, 1340, 1364, 1541
EarI	CTCTTC	5 cut(s)	314, 1106, 1340, 1364, 1541
Eco130I	CCWWGG	2 cut(s)	115, 680
Eco32I	GATATC	3 cut(s)	826, 1580, 1800
Eco47I	GGWCC	2 cut(s)	669, 1435
Eco57I	CTGAAG	2 cut(s)	1364, 1726
Eco88I	CYCGRG	1 cut(s)	2224
EcoNI	CCTNNNNNAGG	1 cut(s)	684
EcoO109I	RGGNCCY	1 cut(s)	843
EcoRI	GAATTC	1 cut(s)	457
EcoRII	CCWGG	3 cut(s)	190, 566, 592
EcoRV	GATATC	3 cut(s)	826, 1580, 1800
EcoT14I	CCWWGG	2 cut(s)	115, 680
EcoT22I	ATGCAT	1 cut(s)	1643
ErhI	CCWWGG	2 cut(s)	115, 680
Esp3I	CGTCTC	1 cut(s)	1463
FaeI	CATG	7 cut(s)	37, 86, 820, 1513, 1765, 2022, 2034
FaqI	GGGAC	4 cut(s)	667, 1351, 1609, 1783
FatI	CATG	7 cut(s)	33, 82, 816, 1509, 1761, 2018, 2030
FauI	CCCGC	2 cut(s)	509, 668
FauNDI	CATATG	1 cut(s)	473
FblI	GTMKAC	1 cut(s)	89
Fnu4HI	GCNGC	7 cut(s)	13, 78, 163, 411, 509, 1089, 2221
FokI	GGATG	2 cut(s)	1694, 1907
Fsp4HI	GCNGC	7 cut(s)	13, 78, 163, 411, 509, 1089, 2221
FspBI	CTAG	4 cut(s)	608, 1526, 1575, 2159
GluI	GCNGC	7 cut(s)	13, 78, 163, 411, 509, 1089, 2221
GsaI	CCCAGC	1 cut(s)	590
GsuI	CTGGAG	2 cut(s)	174, 589
HaeIII	GGCC	5 cut(s)	120, 260, 679, 845, 2065
HgaI	GACGC	1 cut(s)	84
Hin1I	GRCGYC	1 cut(s)	95
Hin1II	CATG	7 cut(s)	37, 86, 820, 1513, 1765, 2022, 2034
HincII	GTYRAC	2 cut(s)	90, 363
HindII	GTYRAC	2 cut(s)	90, 363
HindIII	AAGCTT	1 cut(s)	521
HinfI	GANTC	9 cut(s)	921, 1423, 1491, 1529, 1549, 2100, 2155, 2165, 2207
HphI	GGTGA	3 cut(s)	147, 482, 2174
Hpy166II	GTNNAC	4 cut(s)	90, 363, 876, 978
Hpy188I	TCNGA	9 cut(s)	283, 757, 901, 1051, 1096, 1228, 1334, 1554, 2206
Hpy8I	GTNNAC	4 cut(s)	90, 363, 876, 978
Hpy99I	CGWCG	1 cut(s)	94
HpyAV	CCTTC	7 cut(s)	311, 853, 1232, 1304, 1866, 1979, 2090
HpyCH4III	ACNGT	6 cut(s)	247, 998, 1060, 1382, 1652, 2133
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1638, 1691
HpyF3I	CTNAG	4 cut(s)	26, 379, 1227, 1271
Hsp92I	GRCGYC	1 cut(s)	95
Hsp92II	CATG	7 cut(s)	37, 86, 820, 1513, 1765, 2022, 2034
Kpn2I	TCCGGA	2 cut(s)	709, 1789
KspI	CCGCGG	1 cut(s)	1465
Kzo9I	GATC	8 cut(s)	283, 373, 487, 612, 950, 1860, 1885, 2107
LmnI	GCTCC	2 cut(s)	1792, 2246
Lsp1109I	GCAGC	5 cut(s)	24, 422, 495, 1100, 2207
LweI	GCATC	6 cut(s)	228, 439, 775, 1100, 1650, 1672
MaeI	CTAG	4 cut(s)	608, 1526, 1575, 2159
MaeIII	GTNAC	4 cut(s)	29, 194, 733, 1652
MalI	GATC	8 cut(s)	285, 375, 489, 614, 952, 1862, 1887, 2109
MboI	GATC	8 cut(s)	283, 373, 487, 612, 950, 1860, 1885, 2107
MflI	RGATCY	2 cut(s)	1885, 2107
MlsI	TGGCCA	1 cut(s)	260
MluCI	AATT	9 cut(s)	391, 457, 714, 724, 761, 1188, 1403, 1564, 1752
MluNI	TGGCCA	1 cut(s)	260
MlyI	GAGTC	5 cut(s)	1417, 1558, 2109, 2164, 2174
MmeI	TCCRAC	2 cut(s)	1659, 1950
Mox20I	TGGCCA	1 cut(s)	260
Mph1103I	ATGCAT	1 cut(s)	1643
MroI	TCCGGA	2 cut(s)	709, 1789
MroXI	GAANNNNTTC	1 cut(s)	1671
MscI	TGGCCA	1 cut(s)	260
MseI	TTAA	7 cut(s)	326, 699, 1044, 1377, 1446, 1940, 2251
MslI	CAYNNNNRTG	2 cut(s)	1364, 2035
Msp20I	TGGCCA	1 cut(s)	260
MspA1I	CMGCKG	2 cut(s)	675, 1464
MspR9I	CCNGG	7 cut(s)	192, 513, 568, 594, 832, 1265, 1748
Mva1269I	GAATGC	2 cut(s)	1643, 2040
MvaI	CCWGG	3 cut(s)	192, 568, 594
MvnI	CGCG	2 cut(s)	162, 1464
MwoI	GCNNNNNNNGC	2 cut(s)	1638, 1691
NciI	CCSGG	4 cut(s)	513, 832, 1265, 1748
NdeI	CATATG	1 cut(s)	473
NdeII	GATC	8 cut(s)	283, 373, 487, 612, 950, 1860, 1885, 2107
NlaIII	CATG	7 cut(s)	37, 86, 820, 1513, 1765, 2022, 2034
NlaIV	GGNNCC	2 cut(s)	322, 1101
NmeAIII	GCCGAG	1 cut(s)	2091
NmuCI	GTSAC	2 cut(s)	733, 1652
NsiI	ATGCAT	1 cut(s)	1643
NspI	RCATGY	5 cut(s)	37, 820, 1513, 2022, 2034
PaeI	GCATGC	1 cut(s)	2022
PaeR7I	CTCGAG	1 cut(s)	2224
PciI	ACATGT	1 cut(s)	33
PcsI	WCGNNNNNNNCGW	1 cut(s)	980
PctI	GAATGC	2 cut(s)	1643, 2040
PdmI	GAANNNNTTC	1 cut(s)	1671
PfeI	GAWTC	4 cut(s)	921, 1491, 1529, 2207
PflFI	GACNNNGTC	1 cut(s)	94
PflMI	CCANNNNNTGG	1 cut(s)	593
PinAI	ACCGGT	1 cut(s)	2142
PkrI	GCNGC	7 cut(s)	14, 79, 164, 412, 510, 1090, 2222
PleI	GAGTC	5 cut(s)	1417, 1557, 2108, 2163, 2173
PpsI	GAGTC	5 cut(s)	1417, 1557, 2108, 2163, 2173
PscI	ACATGT	1 cut(s)	33
PshBI	ATTAAT	1 cut(s)	1446
Psp6I	CCWGG	3 cut(s)	190, 566, 592
PspFI	CCCAGC	1 cut(s)	586
PspGI	CCWGG	3 cut(s)	190, 566, 592
PspN4I	GGNNCC	2 cut(s)	322, 1101
PspPI	GGNCC	4 cut(s)	669, 677, 843, 1435
PspXI	VCTCGAGB	1 cut(s)	2224
PstNI	CAGNNNCTG	1 cut(s)	593
PsuI	RGATCY	2 cut(s)	1885, 2107
PsyI	GACNNNGTC	1 cut(s)	94
RsaI	GTAC	6 cut(s)	227, 815, 979, 1149, 1262, 2197
RsaNI	GTAC	6 cut(s)	226, 814, 978, 1148, 1261, 2196
RseI	CAYNNNNRTG	2 cut(s)	1364, 2035
SacII	CCGCGG	1 cut(s)	1465
SalI	GTCGAC	1 cut(s)	88
SaqAI	TTAA	7 cut(s)	326, 699, 1044, 1377, 1446, 1940, 2251
SatI	GCNGC	7 cut(s)	13, 78, 163, 411, 509, 1089, 2221
Sau3AI	GATC	8 cut(s)	283, 373, 487, 612, 950, 1860, 1885, 2107
Sau96I	GGNCC	4 cut(s)	669, 677, 843, 1435
ScaI	AGTACT	2 cut(s)	1149, 2197
SchI	GAGTC	5 cut(s)	1417, 1558, 2109, 2164, 2174
ScrFI	CCNGG	7 cut(s)	192, 513, 568, 594, 832, 1265, 1748
SfaNI	GCATC	6 cut(s)	228, 439, 775, 1100, 1650, 1672
Sfr274I	CTCGAG	1 cut(s)	2224
Sfr303I	CCGCGG	1 cut(s)	1465
SgrBI	CCGCGG	1 cut(s)	1465
SinI	GGWCC	2 cut(s)	669, 1435
SlaI	CTCGAG	1 cut(s)	2224
SmiMI	CAYNNNNRTG	2 cut(s)	1364, 2035
SmlI	CTYRAG	2 cut(s)	961, 2224
SmoI	CTYRAG	2 cut(s)	961, 2224
SphI	GCATGC	1 cut(s)	2022
Sse9I	AATT	9 cut(s)	391, 457, 714, 724, 761, 1188, 1403, 1564, 1752
SsiI	CCGC	8 cut(s)	78, 162, 516, 675, 927, 1356, 1462, 1464
SspI	AATATT	1 cut(s)	1672
SspMI	CTAG	4 cut(s)	608, 1526, 1575, 2159
StyD4I	CCNGG	7 cut(s)	190, 511, 566, 592, 830, 1263, 1746
StyI	CCWWGG	2 cut(s)	115, 680
TaaI	ACNGT	6 cut(s)	247, 998, 1060, 1382, 1652, 2133
TaqI	TCGA	9 cut(s)	89, 171, 336, 486, 615, 939, 1959, 2139, 2225
TaqII	GACCGA	1 cut(s)	512
TasI	AATT	9 cut(s)	391, 457, 714, 724, 761, 1188, 1403, 1564, 1752
TatI	WGTACW	3 cut(s)	977, 1147, 2195
TauI	GCSGC	2 cut(s)	80, 165
TfiI	GAWTC	4 cut(s)	921, 1491, 1529, 2207
Tru1I	TTAA	7 cut(s)	326, 699, 1044, 1377, 1446, 1940, 2251
Tru9I	TTAA	7 cut(s)	326, 699, 1044, 1377, 1446, 1940, 2251
TscAI	CASTG	7 cut(s)	406, 420, 878, 1003, 1347, 1371, 1655
TseFI	GTSAC	2 cut(s)	733, 1652
TseI	GCWGC	5 cut(s)	12, 410, 508, 1088, 2220
Tsp45I	GTSAC	2 cut(s)	733, 1652
TspDTI	ATGAA	6 cut(s)	20, 774, 870, 1215, 1251, 1610
TspGWI	ACGGA	3 cut(s)	333, 639, 1857
TspRI	CASTG	7 cut(s)	406, 420, 878, 1003, 1347, 1371, 1655
Tth111I	GACNNNGTC	1 cut(s)	94
Van91I	CCANNNNNTGG	1 cut(s)	593
VpaK11BI	GGWCC	2 cut(s)	669, 1435
VspI	ATTAAT	1 cut(s)	1446
XagI	CCTNNNNNAGG	1 cut(s)	684
XapI	RAATTY	3 cut(s)	457, 714, 761
XbaI	TCTAGA	2 cut(s)	607, 1525
XceI	RCATGY	5 cut(s)	37, 820, 1513, 2022, 2034
XhoI	CTCGAG	1 cut(s)	2224
XmiI	GTMKAC	1 cut(s)	89
XmnI	GAANNNNTTC	1 cut(s)	1671
XspI	CTAG	4 cut(s)	608, 1526, 1575, 2159
ZrmI	AGTACT	2 cut(s)	1149, 2197
Zsp2I	ATGCAT	1 cut(s)	1643

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.