MD14G1034400.v1.1

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0000916
Physical Location & Seq
Reverse (-)
3041230 .. 3041955
726 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1034400.v1.1.491

Sequence Viewer

Length: 726 bp
ATGGGAACTTTGTTATTAACAGATGCATTCGATTCATCCGTTGTTATATGGCAGAGTTTCAATCACCTGACGAATACTTGGTTGCCGGGTGCTGTTAGATACAATAATCGAACCAAGAGAAAAACAAGTTCTTGGAACCCCGAGAAACCTGGAAAATCCTGCACCTGGTCCTTTTTTTTTTTTGAGCTAGATGAATTACCTTTTAATTTGTCTAACTATCTTGGTACCTTTACTAAAGCCACGCTTGAAATAAATGGTCAGCTCAAGTTTTATGCCTGGTACCAAGACTGCAGGACATGGAAATTGATTTCAACTGAACCATCAAACCAATGGGAGGTCAATGTGTCATGTGGTACTTTTAGTATTTGCGACCAGCGGTGGTATCCTCATTATCGTTGCCTCGAAGGATTTGAACCCAAAGTTAGGGGAAATTGGAGACTAAAGGAATTCTCAGATGGCTGTGTGAGGAAAATTCCTTTTCACTGCAGTCCTGGGGACTCTTTTTTGGTGATAACTGATGTTGCATATCCTCAGAACTTCAAGGAAATTTTCCATGTGAGCATTGATCAATGCAGATTAGAATGCTTAATAAATTTCTCATGCATTGCCATTGCGTATACTTTTTCAGATAGTCGTCGGTGTTACATGTGGACAAGGGATCTATATAATATAAGGACAACACCATACGGATTCGATTTCATAAGTTATTCTCCAAACAACACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_2 Pkinase S_locus_glycop

Protein Analysis

242

Amino Acids

28.39

Weight (kDa)

7.97

Isoelectric Point (pI)

44.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S_locus_glycop PF00954 76 - 140 1.4e-11 S-locus glycoprotein domain
PAN_2 PF08276 162 - 224 2.3e-10 PAN-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 224, 279
AccB1I GGYRCC 2 cut(s) 224, 279
AccI GTMKAC 1 cut(s) 617
AciI CCGC 1 cut(s) 376
AclWI GGATC 1 cut(s) 666
AcsI RAATTY 4 cut(s) 446, 471, 546, 592
AfaI GTAC 3 cut(s) 226, 281, 355
AfiI CCNNNNNNNGG 3 cut(s) 165, 334, 423
AflIII ACRYGT 1 cut(s) 645
AgsI TTSAA 5 cut(s) 61, 248, 312, 413, 541
AjnI CCWGG 4 cut(s) 148, 164, 275, 490
AluBI AGCT 2 cut(s) 187, 262
AluI AGCT 2 cut(s) 187, 262
Alw26I GTCTC 1 cut(s) 430
AlwI GGATC 1 cut(s) 666
Ama87I CYCGRG 1 cut(s) 140
ApoI RAATTY 4 cut(s) 446, 471, 546, 592
Asp718I GGTACC 2 cut(s) 224, 279
AspS9I GGNCC 1 cut(s) 168
AsuC2I CCSGG 1 cut(s) 87
AsuHPI GGTGA 2 cut(s) 56, 520
AvaI CYCGRG 1 cut(s) 140
AvaII GGWCC 1 cut(s) 168
BanI GGYRCC 2 cut(s) 224, 279
BccI CCATC 2 cut(s) 328, 449
BciT130I CCWGG 4 cut(s) 150, 166, 277, 492
BciVI GTATCC 1 cut(s) 393
BclI TGATCA 1 cut(s) 565
BcnI CCSGG 1 cut(s) 87
BcoDI GTCTC 1 cut(s) 430
BfaI CTAG 1 cut(s) 188
BfmI CTRYAG 2 cut(s) 289, 484
BfuI GTATCC 1 cut(s) 393
Bme1390I CCNGG 5 cut(s) 87, 150, 166, 277, 492
Bme18I GGWCC 1 cut(s) 168
BmeT110I CYCGRG 1 cut(s) 140
BmgT120I GGNCC 1 cut(s) 168
BmiI GGNNCC 3 cut(s) 137, 226, 281
BmrFI CCNGG 5 cut(s) 87, 150, 166, 277, 492
BmsI GCATC 1 cut(s) 13
BpuEI CTTGAG 1 cut(s) 248
BpuMI CCSGG 1 cut(s) 87
BsaJI CCNNGG 1 cut(s) 491
Bsc4I CCNNNNNNNGG 3 cut(s) 165, 334, 423
Bse3DI GCAATG 2 cut(s) 603, 609
BseBI CCWGG 4 cut(s) 150, 166, 277, 492
BseDI CCNNGG 1 cut(s) 491
BseGI GGATG 1 cut(s) 35
BseLI CCNNNNNNNGG 3 cut(s) 165, 334, 423
BseMI GCAATG 2 cut(s) 603, 609
BseMII CTCAG 2 cut(s) 465, 545
BsgI GTGCAG 1 cut(s) 145
BshNI GGYRCC 2 cut(s) 224, 279
BsiHKCI CYCGRG 1 cut(s) 140
BsiSI CCGG 1 cut(s) 86
BslFI GGGAC 1 cut(s) 509
BslI CCNNNNNNNGG 3 cut(s) 165, 334, 423
BsmAI GTCTC 1 cut(s) 430
BsmFI GGGAC 1 cut(s) 509
BsmI GAATGC 2 cut(s) 26, 587
BsoBI CYCGRG 1 cut(s) 140
Bsp143I GATC 2 cut(s) 565, 658
BspACI CCGC 1 cut(s) 376
BspCNI CTCAG 2 cut(s) 464, 544
BspLI GGNNCC 3 cut(s) 137, 226, 281
BspMAI CTGCAG 2 cut(s) 293, 488
BspPI GGATC 1 cut(s) 666
BspT107I GGYRCC 2 cut(s) 224, 279
BsrDI GCAATG 2 cut(s) 603, 609
BssECI CCNNGG 1 cut(s) 491
BssMI GATC 2 cut(s) 565, 658
BssNAI GTATAC 1 cut(s) 618
Bst1107I GTATAC 1 cut(s) 618
Bst2UI CCWGG 4 cut(s) 150, 166, 277, 492
BstDEI CTNAG 2 cut(s) 451, 531
BstF5I GGATG 1 cut(s) 35
BstKTI GATC 2 cut(s) 568, 661
BstMAI GTCTC 1 cut(s) 430
BstMBI GATC 2 cut(s) 565, 658
BstNI CCWGG 4 cut(s) 150, 166, 277, 492
BstNSI RCATGY 1 cut(s) 649
BstSCI CCNGG 5 cut(s) 85, 148, 164, 275, 490
BstSFI CTRYAG 2 cut(s) 289, 484
BstX2I RGATCY 1 cut(s) 658
BstYI RGATCY 1 cut(s) 658
BstZ17I GTATAC 1 cut(s) 618
BsuI GTATCC 1 cut(s) 393
BtsCI GGATG 1 cut(s) 35
BtsI GCAGTG 1 cut(s) 481
BtsIMutI CAGTG 1 cut(s) 481
Cfr13I GGNCC 1 cut(s) 168
CsiI ACCWGGT 1 cut(s) 164
Csp6I GTAC 3 cut(s) 225, 280, 354
CviAII CATG 6 cut(s) 297, 348, 554, 600, 646, 723
CviJI RGCY 4 cut(s) 187, 239, 262, 459
CviKI_1 RGCY 4 cut(s) 187, 239, 262, 459
CviQI GTAC 3 cut(s) 225, 280, 354
DdeI CTNAG 2 cut(s) 451, 531
DpnI GATC 2 cut(s) 567, 660
DpnII GATC 2 cut(s) 565, 658
Eco47I GGWCC 1 cut(s) 168
Eco88I CYCGRG 1 cut(s) 140
EcoRI GAATTC 1 cut(s) 446
EcoRII CCWGG 4 cut(s) 148, 164, 275, 490
EcoT22I ATGCAT 2 cut(s) 28, 605
FaeI CATG 6 cut(s) 300, 351, 557, 603, 649, 726
FalI AAGNNNNNCTT 2 cut(s) 228, 260
FaqI GGGAC 1 cut(s) 509
FatI CATG 6 cut(s) 296, 347, 553, 599, 645, 722
FbaI TGATCA 1 cut(s) 565
FblI GTMKAC 1 cut(s) 617
FokI GGATG 1 cut(s) 22
FspBI CTAG 1 cut(s) 188
HapII CCGG 1 cut(s) 86
Hin1II CATG 6 cut(s) 300, 351, 557, 603, 649, 726
HinfI GANTC 3 cut(s) 32, 497, 690
HpaII CCGG 1 cut(s) 86
HphI GGTGA 2 cut(s) 56, 520
Hpy166II GTNNAC 2 cut(s) 618, 651
Hpy188I TCNGA 3 cut(s) 454, 534, 628
Hpy8I GTNNAC 2 cut(s) 618, 651
Hpy99I CGWCG 1 cut(s) 639
HpyAV CCTTC 1 cut(s) 398
HpyCH4V TGCA 7 cut(s) 26, 162, 291, 486, 524, 573, 603
HpyF3I CTNAG 2 cut(s) 451, 531
Hsp92II CATG 6 cut(s) 300, 351, 557, 603, 649, 726
KpnI GGTACC 2 cut(s) 228, 283
Ksp22I TGATCA 1 cut(s) 565
Kzo9I GATC 2 cut(s) 565, 658
LweI GCATC 1 cut(s) 13
MabI ACCWGGT 1 cut(s) 164
MaeI CTAG 1 cut(s) 188
MaeIII GTNAC 1 cut(s) 641
MalI GATC 2 cut(s) 567, 660
MboI GATC 2 cut(s) 565, 658
MflI RGATCY 1 cut(s) 658
MluCI AATT 8 cut(s) 194, 205, 302, 430, 446, 471, 546, 592
MlyI GAGTC 1 cut(s) 491
MnlI CCTC 5 cut(s) 328, 396, 410, 459, 540
Mph1103I ATGCAT 2 cut(s) 28, 605
MseI TTAA 3 cut(s) 17, 204, 587
MspA1I CMGCKG 1 cut(s) 376
MspI CCGG 1 cut(s) 86
MspR9I CCNGG 5 cut(s) 87, 150, 166, 277, 492
Mva1269I GAATGC 2 cut(s) 26, 587
MvaI CCWGG 4 cut(s) 150, 166, 277, 492
NciI CCSGG 1 cut(s) 87
NdeII GATC 2 cut(s) 565, 658
NlaIII CATG 6 cut(s) 300, 351, 557, 603, 649, 726
NlaIV GGNNCC 3 cut(s) 137, 226, 281
NsiI ATGCAT 2 cut(s) 28, 605
NspI RCATGY 1 cut(s) 649
PciI ACATGT 1 cut(s) 645
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PctI GAATGC 2 cut(s) 26, 587
PfeI GAWTC 2 cut(s) 32, 690
PleI GAGTC 1 cut(s) 491
PpsI GAGTC 1 cut(s) 491
PscI ACATGT 1 cut(s) 645
Psp6I CCWGG 4 cut(s) 148, 164, 275, 490
PspGI CCWGG 4 cut(s) 148, 164, 275, 490
PspN4I GGNNCC 3 cut(s) 137, 226, 281
PspPI GGNCC 1 cut(s) 168
PstI CTGCAG 2 cut(s) 293, 488
PsuI RGATCY 1 cut(s) 658
RsaI GTAC 3 cut(s) 226, 281, 355
RsaNI GTAC 3 cut(s) 225, 280, 354
SaqAI TTAA 3 cut(s) 17, 204, 587
Sau3AI GATC 2 cut(s) 565, 658
Sau96I GGNCC 1 cut(s) 168
SchI GAGTC 1 cut(s) 491
ScrFI CCNGG 5 cut(s) 87, 150, 166, 277, 492
SetI ASST 8 cut(s) 69, 151, 167, 189, 202, 230, 264, 339
SexAI ACCWGGT 1 cut(s) 164
SfaNI GCATC 1 cut(s) 13
SfcI CTRYAG 2 cut(s) 289, 484
SinI GGWCC 1 cut(s) 168
SmlI CTYRAG 1 cut(s) 263
SmoI CTYRAG 1 cut(s) 263
Sse9I AATT 8 cut(s) 194, 205, 302, 430, 446, 471, 546, 592
SsiI CCGC 1 cut(s) 376
SspMI CTAG 1 cut(s) 188
StyD4I CCNGG 5 cut(s) 85, 148, 164, 275, 490
TaqI TCGA 4 cut(s) 30, 109, 402, 693
TasI AATT 8 cut(s) 194, 205, 302, 430, 446, 471, 546, 592
TfiI GAWTC 2 cut(s) 32, 690
Tru1I TTAA 3 cut(s) 17, 204, 587
Tru9I TTAA 3 cut(s) 17, 204, 587
TscAI CASTG 1 cut(s) 488
TspDTI ATGAA 3 cut(s) 24, 207, 688
TspGWI ACGGA 2 cut(s) 28, 702
TspRI CASTG 1 cut(s) 488
VpaK11BI GGWCC 1 cut(s) 168
XapI RAATTY 4 cut(s) 446, 471, 546, 592
XceI RCATGY 1 cut(s) 649
XcmI CCANNNNNNNNNTGG 1 cut(s) 327
XmiI GTMKAC 1 cut(s) 617
XspI CTAG 1 cut(s) 188
Zsp2I ATGCAT 2 cut(s) 28, 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.