MD14G1034900.v1.1

G-type lectin S-receptor-like serine threonine-protein kinase At2g19130

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0000916
Physical Location & Seq
Reverse (-)
3123658 .. 3124278
621 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1034900.v1.1.491

Sequence Viewer

Length: 555 bp
ATGCCAAATGGTTCTCTGCAATCTCTTCTACTTGGAAAGAACCCCATAATGTTGGATTGGAACGCTAGATATAATCTTGCAATAGGGACTGCCAAAGGATTGGCTTACCTCCATGAAGAGTGCAGAAACTGCATAATACACTGGGATGTCAAGCCGGAGAACATCTTGTTGGATTCAGAATACAGTCCGAAATTGGCGGATTTCGGTCTTGCAAAACTCATGGGCAGACACCACAGCCGGGTTTTGACCACGATGGGAGGAACTGTAGGATATTTTGCACCGGAATGGTTTTCGGGGATGAGAAACAGAGAAGGGTTAGATGGGGTGGAAAACTTCCGTCCTATCCAGGTCTCGAATGTAGTTAATAAAGGGGAAGATGTTTTCACCTTGTTGGATTGCAGGTTGGAAGGCCAAGCTAACGGAGATGAACTAAGTAGAGCATGCAAAGTGGCTTGTTGGTGTATTCAAGAAGATGAGAAGGATAGGCCAACAATGAGGCAGGTGGTTCAAATTCTCGAGGGAGTTTCGGACATCGGTGTTCCTCCAATCCCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

185

Amino Acids

20.54

Weight (kDa)

5.39

Isoelectric Point (pI)

41.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 1 - 169 1.9e-20 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 1 - 100 4.2e-11 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 490
Acc36I ACCTGC 2 cut(s) 390, 490
AciI CCGC 1 cut(s) 197
AcsI RAATTY 1 cut(s) 510
AfiI CCNNNNNNNGG 1 cut(s) 238
AgsI TTSAA 2 cut(s) 467, 509
AjnI CCWGG 1 cut(s) 345
AjuI GAANNNNNNNTTGG 2 cut(s) 152, 184
AluBI AGCT 1 cut(s) 416
AluI AGCT 1 cut(s) 416
Alw26I GTCTC 1 cut(s) 355
AlwNI CAGNNNCTG 1 cut(s) 129
Ama87I CYCGRG 1 cut(s) 515
AoxI GGCC 2 cut(s) 409, 485
ApoI RAATTY 1 cut(s) 510
AsuC2I CCSGG 1 cut(s) 239
AsuHPI GGTGA 1 cut(s) 376
AvaI CYCGRG 1 cut(s) 515
BccI CCATC 2 cut(s) 247, 314
BciT130I CCWGG 1 cut(s) 347
BcnI CCSGG 1 cut(s) 239
BcoDI GTCTC 1 cut(s) 355
BfaI CTAG 1 cut(s) 66
BfmI CTRYAG 1 cut(s) 264
BfuAI ACCTGC 2 cut(s) 390, 490
Bme1390I CCNGG 2 cut(s) 239, 347
BmeT110I CYCGRG 1 cut(s) 515
BmrFI CCNGG 2 cut(s) 239, 347
BmrI ACTGGG 1 cut(s) 151
BmuI ACTGGG 1 cut(s) 151
BpuMI CCSGG 1 cut(s) 239
BsaI GGTCTC 1 cut(s) 355
BsaWI WCCGGW 1 cut(s) 280
BsaXI ACNNNNNCTCC 2 cut(s) 249, 279
Bsc4I CCNNNNNNNGG 1 cut(s) 238
Bse1I ACTGG 1 cut(s) 146
BseBI CCWGG 1 cut(s) 347
BseGI GGATG 2 cut(s) 151, 303
BseLI CCNNNNNNNGG 1 cut(s) 238
BseNI ACTGG 1 cut(s) 146
BsgI GTGCAG 1 cut(s) 142
BshFI GGCC 2 cut(s) 411, 487
BsiHKCI CYCGRG 1 cut(s) 515
BsiSI CCGG 3 cut(s) 155, 238, 281
BslFI GGGAC 1 cut(s) 100
BslI CCNNNNNNNGG 1 cut(s) 238
BsmAI GTCTC 1 cut(s) 355
BsmFI GGGAC 1 cut(s) 100
BsnI GGCC 2 cut(s) 411, 487
Bso31I GGTCTC 1 cut(s) 355
BsoBI CYCGRG 1 cut(s) 515
BspACI CCGC 1 cut(s) 197
BspANI GGCC 2 cut(s) 411, 487
BspMI ACCTGC 2 cut(s) 390, 490
BspTNI GGTCTC 1 cut(s) 355
BsrI ACTGG 1 cut(s) 146
Bst2UI CCWGG 1 cut(s) 347
Bst4CI ACNGT 2 cut(s) 185, 265
Bst6I CTCTTC 2 cut(s) 30, 111
BstAPI GCANNNNNTGC 1 cut(s) 129
BstC8I GCNNGC 1 cut(s) 442
BstDEI CTNAG 1 cut(s) 431
BstF5I GGATG 2 cut(s) 151, 303
BstMAI GTCTC 1 cut(s) 355
BstMWI GCNNNNNNNGC 1 cut(s) 129
BstNI CCWGG 1 cut(s) 347
BstNSI RCATGY 1 cut(s) 444
BstSCI CCNGG 2 cut(s) 237, 345
BstSFI CTRYAG 1 cut(s) 264
BstXI CCANNNNNNTGG 2 cut(s) 52, 100
BsuRI GGCC 2 cut(s) 411, 487
BtsCI GGATG 2 cut(s) 151, 303
BtsIMutI CAGTG 1 cut(s) 139
BveI ACCTGC 2 cut(s) 390, 490
Cac8I GCNNGC 1 cut(s) 442
CaiI CAGNNNCTG 1 cut(s) 129
CviAII CATG 3 cut(s) 113, 220, 441
CviJI RGCY 7 cut(s) 104, 154, 237, 411, 416, 452, 487
CviKI_1 RGCY 7 cut(s) 104, 154, 237, 411, 416, 452, 487
DdeI CTNAG 1 cut(s) 431
Eam1104I CTCTTC 2 cut(s) 30, 111
EarI CTCTTC 2 cut(s) 30, 111
EciI GGCGGA 1 cut(s) 212
Eco31I GGTCTC 1 cut(s) 355
Eco88I CYCGRG 1 cut(s) 515
EcoRII CCWGG 1 cut(s) 345
FaeI CATG 3 cut(s) 116, 223, 444
FaiI YATR 6 cut(s) 47, 72, 114, 134, 221, 442
FaqI GGGAC 1 cut(s) 100
FatI CATG 3 cut(s) 112, 219, 440
FokI GGATG 2 cut(s) 158, 310
FspBI CTAG 1 cut(s) 66
HaeIII GGCC 2 cut(s) 411, 487
HapII CCGG 3 cut(s) 155, 238, 281
Hin1II CATG 3 cut(s) 116, 223, 444
HinfI GANTC 1 cut(s) 173
HpaII CCGG 3 cut(s) 155, 238, 281
HphI GGTGA 1 cut(s) 376
Hpy188I TCNGA 3 cut(s) 178, 189, 529
Hpy188III TCNNGA 3 cut(s) 352, 467, 515
HpyAV CCTTC 3 cut(s) 305, 401, 472
HpyCH4III ACNGT 2 cut(s) 185, 265
HpyCH4V TGCA 8 cut(s) 19, 80, 123, 132, 212, 278, 399, 444
HpyF10VI GCNNNNNNNGC 1 cut(s) 129
HpyF3I CTNAG 1 cut(s) 431
Hsp92II CATG 3 cut(s) 116, 223, 444
LpnPI CCDG 8 cut(s) 127, 168, 251, 294, 332, 359, 385, 485
MaeI CTAG 1 cut(s) 66
MboII GAAGA 4 cut(s) 17, 128, 386, 482
MluCI AATT 2 cut(s) 191, 510
MmeI TCCRAC 4 cut(s) 33, 150, 372, 384
MnlI CCTC 5 cut(s) 119, 251, 489, 511, 552
MseI TTAA 1 cut(s) 363
MslI CAYNNNNRTG 2 cut(s) 144, 283
MspI CCGG 3 cut(s) 155, 238, 281
MspR9I CCNGG 2 cut(s) 239, 347
MvaI CCWGG 1 cut(s) 347
MwoI GCNNNNNNNGC 1 cut(s) 129
NciI CCSGG 1 cut(s) 239
NlaIII CATG 3 cut(s) 116, 223, 444
NspI RCATGY 1 cut(s) 444
PaeI GCATGC 1 cut(s) 444
PaeR7I CTCGAG 1 cut(s) 515
PaqCI CACCTGC 1 cut(s) 490
PfeI GAWTC 1 cut(s) 173
Psp6I CCWGG 1 cut(s) 345
PspGI CCWGG 1 cut(s) 345
PstNI CAGNNNCTG 1 cut(s) 129
RseI CAYNNNNRTG 2 cut(s) 144, 283
SaqAI TTAA 1 cut(s) 363
ScrFI CCNGG 2 cut(s) 239, 347
SetI ASST 6 cut(s) 111, 351, 389, 404, 418, 504
SfcI CTRYAG 1 cut(s) 264
Sfr274I CTCGAG 1 cut(s) 515
SlaI CTCGAG 1 cut(s) 515
SmiMI CAYNNNNRTG 2 cut(s) 144, 283
SmlI CTYRAG 1 cut(s) 515
SmoI CTYRAG 1 cut(s) 515
SphI GCATGC 1 cut(s) 444
Sse9I AATT 2 cut(s) 191, 510
SsiI CCGC 1 cut(s) 197
SspMI CTAG 1 cut(s) 66
StyD4I CCNGG 2 cut(s) 237, 345
TaaI ACNGT 2 cut(s) 185, 265
TaqI TCGA 2 cut(s) 353, 516
TaqII GACCGA 1 cut(s) 194
TasI AATT 2 cut(s) 191, 510
TfiI GAWTC 1 cut(s) 173
Tru1I TTAA 1 cut(s) 363
Tru9I TTAA 1 cut(s) 363
TscAI CASTG 1 cut(s) 146
TspDTI ATGAA 2 cut(s) 129, 441
TspGWI ACGGA 2 cut(s) 326, 435
TspRI CASTG 1 cut(s) 146
XapI RAATTY 1 cut(s) 510
XceI RCATGY 1 cut(s) 444
XhoI CTCGAG 1 cut(s) 515
XspI CTAG 1 cut(s) 66
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.