pycom12g03080

G-type lectin S-receptor-like serine threonine-protein kinase At2g19130

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0000916
Physical Location & Seq
Reverse (-)
2790636 .. 2792942
2307 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g03080.1

Sequence Viewer

Length: 2307 bp
ATGGATACGAAAATTCTACTCTGCTATGCGTTGAGATTTTTGCTATGTTGCACCTTCATTGCTTGTATTTCCACGGCGTCGCACACCATCTCTCCAGGCCATTCTCTCTCCGGGGAACAAACAATCACCTCTCCAAGTGGAAAATTTGAGCTCGGTTTCTTCACACCAGCAATCACCTCTCCAAGCGGAAAATTTGAGCTCGGTTTCTTCCCACCAGGTATACCTCAAAACTATTACGTAGGCATTTGGTACAAAAATCTACCCAATCGGACTCTAGTTTGGGTCGCAAACAGAAAACATCCTGTTTGTGAACCGTATTTGTCAGTATTACGACTTGTCGAAAATGGAAACTTGACCTTAAGTGGCCCCTCCAAAGTTGCAATATGGTCGACTAATTCAAGATCAAGGGTCTTGAATTCTAGTGTAGCAAAGCTTCTTGACAATGGGAACTTTGTCATAACGGATGCATTTAATTCATCTGTTGTCAAATGGCAGAGTTTCGATCACCCGACGGATACTTGGCTCCCGGGTGCTAAGCTTGGATACAATAATCGTACGAGGAGAAAACTAGTTCTTACATCCTGGAGAAATCGGAAAACTCCTGCACCTGGTCCTTTTTCTTCTGAGCTAGATGAAATACCTTTTAATTTATCTTCATATCCTGATACCTTTACTAGAGGCAGACTTGAAATCAATGGTCAGCTAAAGTTCTATGCCTGGGACCAAGGCTTTAGGGAGTGGACTTTGATTTCGGCTGAACCATCGGACCAGTGTGCGGTTGATTCTTCATGCGGTGCTTTTAGTATTTGCAACCAGCGGAGTTCTCCTCGTTGTGGTTGCCTCGAAGGATTTGAACCCAGAGTCAGGGAAAGTTGGATGCTAGAGGACTTCTCAGATGGCTGTGTGAGGAAATTTCCTTTTCACTACAGTCCCGGGGACTCATTTTTGGCGATAACTGATGTTGGAATTGGATATCCTAGGAACTATGAGCAAATTATAAATGTGAGCAATGATCAATGCAGATTAGAATGCTTAATAAATTGCTCATGCAGTGCCTTTGTTTATAATTTTATTGATAGTCGTCGGTGTTTCATTTGGATAGGGGATCTATATAATATACTGACAATGCCATCCGAATCCATTTCCATAAGTTATTCTCCAGACAGCCCAGGATTGCATCTCCGGATTGTGGATTCTACAAGTGAGACGAAGAGAAAGAGAAAGACTACTTGGACTGTAATTGGAGTAATTGCAGGGTTCTTTTCCATCTTAAGTAGTACTTTCATTGTCATGGTATTCTTTAGAAGGAGATGGTCAGCAGGAGCATTGGCGACTACCGACGATTCTTTGGTGCTTTACAAGCATAGAGATTTAAGAAGAGCAACAAACAACTTCTCCGAAAAACTCGGGGAAGGAGGCTTTGGTTCTGTATTCAAGGGGACGTTGCCTAACTCAACGGCCATAGCAGTGAAAGAGCTGAGAAGTATGAACCAGGGAGAGAAACAATTTCGTGCTGAAGTGGGAACTATCGGTGTAATCCAACACATTAATCTTATTCGCATGCGGGGATTCTGCTCCAACGCTTCAAAGAGGTTTTTAGTTTATGACTACATGCCAAATGGTTCTCTGCAATCTCTTATACTTGGAAATAACCCGAGAATGTTAGATTGGAATGCTAGATATCACATTGCAGTAGGGACTGCCAGAGGATTGTCTTACCTCCATGAAGAGTGCAGAGACTGCATAATACACTGCGACGTTAAGCCAGAGAACATCTTGTTGGATTCAGAATACAGTCCGAAATTGGCGGATTTTGGTCTTGCGAAACTCTTGGGGAGAGACCACAGCCGGGTTTTGACAACCATGAGAGGAACTGTGGGCTATCTCGCACCAGAATGGTTTTCGGGTGAAGCCATCACCCCAAAAGCCGATGTGTTTAGCTATGGCATGTTGCTGATTGAGGTCATATCAGGGAGGAGAAACAGAGAAGGATTAGCTGACGGGGTAGAAAACTTCCGTCCTATCCAGCTCGCGAATGCAGTTAATAAAGGGGAAGATCTTTTCACCTTGCTGGATTGCAGGTTGGAAGGCAAAGCTGACAGAGATGACCTAAGTAGAGCGTGCAAAGTGGCTTGTTGGTGCATTCAAGAAGATGAGAAGGATAGGCCAACAATGAGGCAGGTTGTTCAAATTCTTGAGGGAGTTTTGGACATCGGTGTTCCGCCAGTCCCGCAGTTCCTCCAGCGCCTTATCAGAAGTCCGGCAGAAGGCATTAACTACCTGGAAACTACATCCGATTCAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_2 Pkinase S_locus_glycop

Protein Analysis

769

Amino Acids

86.15

Weight (kDa)

8.31

Isoelectric Point (pI)

48.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 91 - 196 4.1e-32 D-mannose binding lectin
S_locus_glycop PF00954 222 - 287 3.1e-15 S-locus glycoprotein domain
PAN_2 PF08276 311 - 373 4.7e-12 PAN-like domain
PK_Tyr_Ser-Thr PF07714 465 - 732 1.1e-40 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 466 - 730 2.8e-48 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 998, 1065
Acc36I ACCTGC 2 cut(s) 2070, 2170
AccI GTMKAC 2 cut(s) 220, 389
AccII CGCG 1 cut(s) 2033
AccIII TCCGGA 1 cut(s) 1182
AciI CCGC 8 cut(s) 186, 776, 792, 817, 1564, 1808, 2222, 2231
AclWI GGATC 1 cut(s) 1113
AcoI YGGCCR 1 cut(s) 1458
AcsI RAATTY 6 cut(s) 12, 143, 191, 415, 911, 2190
AcuI CTGAAG 1 cut(s) 1536
AcyI GRCGYC 1 cut(s) 77
AfaI GTAC 3 cut(s) 251, 556, 1279
AfiI CCNNNNNNNGG 7 cut(s) 608, 775, 833, 864, 1189, 1849, 2267
AflII CTTAAG 2 cut(s) 358, 1270
AgsI TTSAA 8 cut(s) 399, 415, 689, 854, 1435, 1587, 2147, 2189
AhlI ACTAGT 1 cut(s) 568
AjnI CCWGG 8 cut(s) 94, 214, 581, 607, 716, 1168, 1491, 2280
AjuI GAANNNNNNNTTGG 8 cut(s) 1213, 1245, 1404, 1436, 1651, 1683, 1763, 1795
Alw21I GWGCWC 2 cut(s) 153, 201
Alw26I GTCTC 3 cut(s) 1199, 1731, 1833
AlwI GGATC 1 cut(s) 1113
AlwNI CAGNNNCTG 1 cut(s) 1740
Ama87I CYCGRG 4 cut(s) 526, 932, 1406, 1654
Aor13HI TCCGGA 1 cut(s) 1182
AoxI GGCC 4 cut(s) 97, 364, 1458, 2165
ApoI RAATTY 6 cut(s) 12, 143, 191, 415, 911, 2190
ArsI GACNNNNNNTTYG 2 cut(s) 733, 765
AseI ATTAAT 1 cut(s) 1548
AspA2I CCTAGG 1 cut(s) 977
AspLEI GCGC 1 cut(s) 2247
AspS9I GGNCC 4 cut(s) 365, 611, 721, 766
AsuC2I CCSGG 6 cut(s) 112, 527, 528, 933, 934, 1850
AsuHPI GGTGA 6 cut(s) 118, 166, 497, 1909, 1919, 2056
AvaI CYCGRG 4 cut(s) 526, 932, 1406, 1654
AvaII GGWCC 3 cut(s) 611, 721, 766
AvrII CCTAGG 1 cut(s) 977
BanII GRGCYC 2 cut(s) 153, 201
BarI GAAGNNNNNNTAC 2 cut(s) 417, 449
Bbv12I GWGCWC 2 cut(s) 153, 201
BccI CCATC 7 cut(s) 95, 769, 890, 1138, 1274, 1305, 1922
BceAI ACGGC 2 cut(s) 90, 1473
BcgI CGANNNNNNTGC 2 cut(s) 369, 403
BciT130I CCWGG 8 cut(s) 96, 216, 583, 609, 718, 1170, 1493, 2282
BciVI GTATCC 2 cut(s) 508, 536
BclI TGATCA 1 cut(s) 1012
BcnI CCSGG 6 cut(s) 112, 527, 528, 933, 934, 1850
BcoDI GTCTC 3 cut(s) 1199, 1731, 1833
BcuI ACTAGT 1 cut(s) 568
BfaI CTAG 8 cut(s) 275, 420, 569, 629, 675, 881, 978, 1677
BfmI CTRYAG 1 cut(s) 925
BfoI RGCGCY 1 cut(s) 2248
BfrI CTTAAG 2 cut(s) 358, 1270
BfuAI ACCTGC 2 cut(s) 2070, 2170
BfuI GTATCC 2 cut(s) 508, 536
BglII AGATCT 1 cut(s) 2056
BlnI CCTAGG 1 cut(s) 977
BlpI GCTNAGC 1 cut(s) 534
BmcAI AGTACT 1 cut(s) 1279
Bme18I GGWCC 3 cut(s) 611, 721, 766
BmeT110I CYCGRG 4 cut(s) 526, 932, 1406, 1654
BmgT120I GGNCC 4 cut(s) 365, 611, 721, 766
BmiI GGNNCC 3 cut(s) 367, 524, 722
BmsI GCATC 3 cut(s) 454, 867, 1186
BplI GAGNNNNNCTC 4 cut(s) 811, 843, 875, 907
BpmI CTGGAG 4 cut(s) 78, 604, 1143, 2225
Bpu1102I GCTNAGC 1 cut(s) 534
BpuEI CTTGAG 1 cut(s) 2216
BpuMI CCSGG 6 cut(s) 112, 527, 528, 933, 934, 1850
BsaAI YACGTR 1 cut(s) 238
BsaHI GRCGYC 1 cut(s) 77
BsaI GGTCTC 1 cut(s) 1833
BsaWI WCCGGW 1 cut(s) 1182
BsaXI ACNNNNNCTCC 4 cut(s) 76, 106, 1379, 1409
Bsc4I CCNNNNNNNGG 7 cut(s) 608, 775, 833, 864, 1189, 1849, 2267
Bse1I ACTGG 2 cut(s) 769, 2225
Bse3DI GCAATG 3 cut(s) 57, 1015, 1686
BseAI TCCGGA 1 cut(s) 1182
BseBI CCWGG 8 cut(s) 96, 216, 583, 609, 718, 1170, 1493, 2282
BseGI GGATG 6 cut(s) 298, 469, 578, 882, 1130, 2291
BseLI CCNNNNNNNGG 7 cut(s) 608, 775, 833, 864, 1189, 1849, 2267
BseMI GCAATG 3 cut(s) 57, 1015, 1686
BseMII CTCAG 3 cut(s) 615, 906, 1469
BseNI ACTGG 2 cut(s) 769, 2225
BseRI GAGGAG 3 cut(s) 574, 816, 1990
BsgI GTGCAG 2 cut(s) 588, 1753
Bsh1236I CGCG 1 cut(s) 2033
BshFI GGCC 4 cut(s) 99, 366, 1460, 2167
BsiHKAI GWGCWC 2 cut(s) 153, 201
BsiHKCI CYCGRG 4 cut(s) 526, 932, 1406, 1654
BsiSI CCGG 6 cut(s) 111, 527, 933, 1183, 1849, 2261
BsiWI CGTACG 1 cut(s) 554
BslFI GGGAC 6 cut(s) 734, 915, 950, 1453, 1711, 2213
BslI CCNNNNNNNGG 7 cut(s) 608, 775, 833, 864, 1189, 1849, 2267
BsmAI GTCTC 3 cut(s) 1199, 1731, 1833
BsmBI CGTCTC 1 cut(s) 1199
BsmFI GGGAC 6 cut(s) 734, 915, 950, 1453, 1711, 2213
BsmI GAATGC 4 cut(s) 1034, 1678, 2041, 2142
BsnI GGCC 4 cut(s) 99, 366, 1460, 2167
Bso31I GGTCTC 1 cut(s) 1833
BsoBI CYCGRG 4 cut(s) 526, 932, 1406, 1654
Bsp1286I GDGCHC 2 cut(s) 153, 201
Bsp13I TCCGGA 1 cut(s) 1182
Bsp143I GATC 5 cut(s) 401, 502, 1012, 1105, 2056
Bsp1720I GCTNAGC 1 cut(s) 534
Bsp68I TCGCGA 1 cut(s) 2033
BspACI CCGC 8 cut(s) 186, 776, 792, 817, 1564, 1808, 2222, 2231
BspANI GGCC 4 cut(s) 99, 366, 1460, 2167
BspCNI CTCAG 3 cut(s) 616, 905, 1470
BspEI TCCGGA 1 cut(s) 1182
BspFNI CGCG 1 cut(s) 2033
BspLI GGNNCC 3 cut(s) 367, 524, 722
BspMI ACCTGC 2 cut(s) 2070, 2170
BspPI GGATC 1 cut(s) 1113
BspQI GCTCTTC 1 cut(s) 1372
BspTI CTTAAG 2 cut(s) 358, 1270
BspTNI GGTCTC 1 cut(s) 1833
BsrDI GCAATG 3 cut(s) 57, 1015, 1686
BsrI ACTGG 2 cut(s) 769, 2225
BssMI GATC 5 cut(s) 401, 502, 1012, 1105, 2056
BssNAI GTATAC 1 cut(s) 221
BssNI GRCGYC 1 cut(s) 77
BssT1I CCWWGG 2 cut(s) 724, 977
Bst1107I GTATAC 1 cut(s) 221
Bst2UI CCWGG 8 cut(s) 96, 216, 583, 609, 718, 1170, 1493, 2282
Bst4CI ACNGT 5 cut(s) 315, 929, 1237, 1796, 1876
Bst6I CTCTTC 3 cut(s) 1205, 1372, 1722
BstACI GRCGYC 1 cut(s) 77
BstAFI CTTAAG 2 cut(s) 358, 1270
BstAPI GCANNNNNTGC 1 cut(s) 1740
BstBAI YACGTR 1 cut(s) 238
BstC8I GCNNGC 3 cut(s) 1562, 2031, 2122
BstDEI CTNAG 5 cut(s) 534, 624, 892, 1478, 2111
BstDSI CCRYGG 1 cut(s) 72
BstF5I GGATG 6 cut(s) 298, 469, 578, 882, 1130, 2291
BstFNI CGCG 1 cut(s) 2033
BstH2I RGCGCY 1 cut(s) 2248
BstHHI GCGC 1 cut(s) 2247
BstKTI GATC 5 cut(s) 404, 505, 1015, 1108, 2059
BstMAI GTCTC 3 cut(s) 1199, 1731, 1833
BstMBI GATC 5 cut(s) 401, 502, 1012, 1105, 2056
BstMWI GCNNNNNNNGC 3 cut(s) 1360, 1740, 2230
BstNI CCWGG 8 cut(s) 96, 216, 583, 609, 718, 1170, 1493, 2282
BstNSI RCATGY 3 cut(s) 1564, 1615, 1951
BstSFI CTRYAG 1 cut(s) 925
BstSNI TACGTA 1 cut(s) 238
BstUI CGCG 1 cut(s) 2033
BstX2I RGATCY 2 cut(s) 1105, 2056
BstYI RGATCY 2 cut(s) 1105, 2056
BstZ17I GTATAC 1 cut(s) 221
BsuI GTATCC 2 cut(s) 508, 536
BsuRI GGCC 4 cut(s) 99, 366, 1460, 2167
BtgI CCRYGG 1 cut(s) 72
BtsCI GGATG 6 cut(s) 298, 469, 578, 882, 1130, 2291
BtsI GCAGTG 3 cut(s) 1057, 1473, 1750
BtsIMutI CAGTG 4 cut(s) 776, 1057, 1473, 1750
BtuMI TCGCGA 1 cut(s) 2033
BveI ACCTGC 2 cut(s) 2070, 2170
Cac8I GCNNGC 3 cut(s) 1562, 2031, 2122
CaiI CAGNNNCTG 1 cut(s) 1740
CfoI GCGC 1 cut(s) 2247
Cfr13I GGNCC 4 cut(s) 365, 611, 721, 766
Cfr9I CCCGGG 2 cut(s) 526, 932
CseI GACGC 1 cut(s) 66
CsiI ACCWGGT 2 cut(s) 214, 607
Csp6I GTAC 3 cut(s) 250, 555, 1278
CviAII CATG 8 cut(s) 789, 1047, 1291, 1561, 1612, 1724, 1864, 1948
CviQI GTAC 3 cut(s) 250, 555, 1278
DdeI CTNAG 5 cut(s) 534, 624, 892, 1478, 2111
DpnI GATC 5 cut(s) 403, 504, 1014, 1107, 2058
DpnII GATC 5 cut(s) 401, 502, 1012, 1105, 2056
EaeI YGGCCR 1 cut(s) 1458
Eam1104I CTCTTC 3 cut(s) 1205, 1372, 1722
EarI CTCTTC 3 cut(s) 1205, 1372, 1722
EciI GGCGGA 2 cut(s) 1823, 2211
Ecl136II GAGCTC 2 cut(s) 151, 199
Eco105I TACGTA 1 cut(s) 238
Eco130I CCWWGG 2 cut(s) 724, 977
Eco24I GRGCYC 2 cut(s) 153, 201
Eco31I GGTCTC 1 cut(s) 1833
Eco32I GATATC 2 cut(s) 974, 1682
Eco47I GGWCC 3 cut(s) 611, 721, 766
Eco53kI GAGCTC 2 cut(s) 151, 199
Eco57I CTGAAG 1 cut(s) 1536
Eco88I CYCGRG 4 cut(s) 526, 932, 1406, 1654
EcoICRI GAGCTC 2 cut(s) 151, 199
EcoRI GAATTC 1 cut(s) 415
EcoRII CCWGG 8 cut(s) 94, 214, 581, 607, 716, 1168, 1491, 2280
EcoRV GATATC 2 cut(s) 974, 1682
EcoT14I CCWWGG 2 cut(s) 724, 977
EcoT22I ATGCAT 1 cut(s) 469
EcoT38I GRGCYC 2 cut(s) 153, 201
ErhI CCWWGG 2 cut(s) 724, 977
Esp3I CGTCTC 1 cut(s) 1199
FaeI CATG 8 cut(s) 792, 1050, 1294, 1564, 1615, 1727, 1867, 1951
FalI AAGNNNNNCTT 2 cut(s) 1264, 1296
FaqI GGGAC 6 cut(s) 734, 915, 950, 1453, 1711, 2213
FatI CATG 8 cut(s) 788, 1046, 1290, 1560, 1611, 1723, 1863, 1947
FauI CCCGC 2 cut(s) 1557, 2238
FbaI TGATCA 1 cut(s) 1012
FblI GTMKAC 2 cut(s) 220, 389
FokI GGATG 6 cut(s) 285, 476, 565, 889, 1117, 2278
FriOI GRGCYC 2 cut(s) 153, 201
FspBI CTAG 8 cut(s) 275, 420, 569, 629, 675, 881, 978, 1677
GlaI GCGC 1 cut(s) 2246
GsuI CTGGAG 4 cut(s) 78, 604, 1143, 2225
HaeII RGCGCY 1 cut(s) 2248
HaeIII GGCC 4 cut(s) 99, 366, 1460, 2167
HapII CCGG 6 cut(s) 111, 527, 933, 1183, 1849, 2261
HgaI GACGC 1 cut(s) 66
HhaI GCGC 1 cut(s) 2247
Hin1I GRCGYC 1 cut(s) 77
Hin1II CATG 8 cut(s) 792, 1050, 1294, 1564, 1615, 1727, 1867, 1951
Hin6I GCGC 1 cut(s) 2245
HinP1I GCGC 1 cut(s) 2245
HincII GTYRAC 1 cut(s) 390
HindII GTYRAC 1 cut(s) 390
HindIII AAGCTT 2 cut(s) 431, 536
HpaII CCGG 6 cut(s) 111, 527, 933, 1183, 1849, 2261
HphI GGTGA 6 cut(s) 118, 166, 497, 1909, 1919, 2056
Hpy166II GTNNAC 4 cut(s) 221, 311, 390, 741
Hpy188III TCNNGA 9 cut(s) 399, 412, 437, 662, 1160, 1183, 2032, 2147, 2195
Hpy8I GTNNAC 4 cut(s) 221, 311, 390, 741
Hpy99I CGWCG 5 cut(s) 82, 514, 1086, 1343, 1760
HpyAV CCTTC 8 cut(s) 64, 839, 1299, 1406, 1982, 2081, 2152, 2261
HpyCH4III ACNGT 5 cut(s) 315, 929, 1237, 1796, 1876
HpyCH4IV ACGT 3 cut(s) 237, 1442, 1758
HpyF10VI GCNNNNNNNGC 3 cut(s) 1360, 1740, 2230
HpyF3I CTNAG 5 cut(s) 534, 624, 892, 1478, 2111
HpySE526I ACGT 3 cut(s) 237, 1442, 1758
Hsp92I GRCGYC 1 cut(s) 77
Hsp92II CATG 8 cut(s) 792, 1050, 1294, 1564, 1615, 1727, 1867, 1951
HspAI GCGC 1 cut(s) 2245
Kpn2I TCCGGA 1 cut(s) 1182
Ksp22I TGATCA 1 cut(s) 1012
Kzo9I GATC 5 cut(s) 401, 502, 1012, 1105, 2056
LguI GCTCTTC 1 cut(s) 1372
LmnI GCTCC 3 cut(s) 528, 1322, 1580
LweI GCATC 3 cut(s) 454, 867, 1186
MabI ACCWGGT 2 cut(s) 214, 607
MaeI CTAG 8 cut(s) 275, 420, 569, 629, 675, 881, 978, 1677
MaeII ACGT 3 cut(s) 237, 1442, 1758
MalI GATC 5 cut(s) 403, 504, 1014, 1107, 2058
MboI GATC 5 cut(s) 401, 502, 1012, 1105, 2056
MflI RGATCY 2 cut(s) 1105, 2056
MhlI GDGCHC 2 cut(s) 153, 201
MlyI GAGTC 3 cut(s) 265, 870, 932
MmeI TCCRAC 6 cut(s) 854, 943, 1564, 1602, 1761, 2064
Mph1103I ATGCAT 1 cut(s) 469
MroI TCCGGA 1 cut(s) 1182
MslI CAYNNNNRTG 3 cut(s) 1289, 1466, 1894
MspA1I CMGCKG 1 cut(s) 817
MspCI CTTAAG 2 cut(s) 358, 1270
MspI CCGG 6 cut(s) 111, 527, 933, 1183, 1849, 2261
Mva1269I GAATGC 4 cut(s) 1034, 1678, 2041, 2142
MvaI CCWGG 8 cut(s) 96, 216, 583, 609, 718, 1170, 1493, 2282
MvnI CGCG 1 cut(s) 2033
MwoI GCNNNNNNNGC 3 cut(s) 1360, 1740, 2230
NciI CCSGG 6 cut(s) 112, 527, 528, 933, 934, 1850
NdeII GATC 5 cut(s) 401, 502, 1012, 1105, 2056
NlaIII CATG 8 cut(s) 792, 1050, 1294, 1564, 1615, 1727, 1867, 1951
NlaIV GGNNCC 3 cut(s) 367, 524, 722
NruI TCGCGA 1 cut(s) 2033
NsiI ATGCAT 1 cut(s) 469
NspI RCATGY 3 cut(s) 1564, 1615, 1951
PaeI GCATGC 1 cut(s) 1564
PciSI GCTCTTC 1 cut(s) 1372
PctI GAATGC 4 cut(s) 1034, 1678, 2041, 2142
PfeI GAWTC 7 cut(s) 782, 1136, 1193, 1343, 1569, 1784, 2297
Pfl23II CGTACG 1 cut(s) 554
PfoI TCCNGGA 1 cut(s) 581
PleI GAGTC 3 cut(s) 265, 869, 932
PpsI GAGTC 3 cut(s) 265, 869, 932
Ppu21I YACGTR 1 cut(s) 238
PshBI ATTAAT 1 cut(s) 1548
PsiI TTATAA 2 cut(s) 998, 1065
Psp124BI GAGCTC 2 cut(s) 153, 201
Psp6I CCWGG 8 cut(s) 94, 214, 581, 607, 716, 1168, 1491, 2280
PspGI CCWGG 8 cut(s) 94, 214, 581, 607, 716, 1168, 1491, 2280
PspLI CGTACG 1 cut(s) 554
PspN4I GGNNCC 3 cut(s) 367, 524, 722
PspPI GGNCC 4 cut(s) 365, 611, 721, 766
PstNI CAGNNNCTG 1 cut(s) 1740
PsuI RGATCY 2 cut(s) 1105, 2056
RruI TCGCGA 1 cut(s) 2033
RsaI GTAC 3 cut(s) 251, 556, 1279
RsaNI GTAC 3 cut(s) 250, 555, 1278
RseI CAYNNNNRTG 3 cut(s) 1289, 1466, 1894
SacI GAGCTC 2 cut(s) 153, 201
SalI GTCGAC 1 cut(s) 388
SapI GCTCTTC 1 cut(s) 1372
Sau3AI GATC 5 cut(s) 401, 502, 1012, 1105, 2056
Sau96I GGNCC 4 cut(s) 365, 611, 721, 766
ScaI AGTACT 1 cut(s) 1279
SchI GAGTC 3 cut(s) 265, 870, 932
SduI GDGCHC 2 cut(s) 153, 201
SexAI ACCWGGT 2 cut(s) 214, 607
SfaNI GCATC 3 cut(s) 454, 867, 1186
SfcI CTRYAG 1 cut(s) 925
SinI GGWCC 3 cut(s) 611, 721, 766
SmaI CCCGGG 2 cut(s) 528, 934
SmiMI CAYNNNNRTG 3 cut(s) 1289, 1466, 1894
SmlI CTYRAG 3 cut(s) 358, 1270, 2195
SmoI CTYRAG 3 cut(s) 358, 1270, 2195
SnaBI TACGTA 1 cut(s) 238
SpeI ACTAGT 1 cut(s) 568
SphI GCATGC 1 cut(s) 1564
SsiI CCGC 8 cut(s) 186, 776, 792, 817, 1564, 1808, 2222, 2231
SspMI CTAG 8 cut(s) 275, 420, 569, 629, 675, 881, 978, 1677
SstI GAGCTC 2 cut(s) 153, 201
StyI CCWWGG 2 cut(s) 724, 977
TaaI ACNGT 5 cut(s) 315, 929, 1237, 1796, 1876
TaiI ACGT 3 cut(s) 240, 1445, 1761
TaqI TCGA 4 cut(s) 339, 389, 501, 843
TatI WGTACW 1 cut(s) 1277
TfiI GAWTC 7 cut(s) 782, 1136, 1193, 1343, 1569, 1784, 2297
TscAI CASTG 4 cut(s) 776, 1057, 1473, 1757
TspDTI ATGAA 9 cut(s) 46, 465, 645, 648, 777, 1081, 1273, 1502, 1740
TspGWI ACGGA 3 cut(s) 476, 527, 2006
TspMI CCCGGG 2 cut(s) 526, 932
TspRI CASTG 4 cut(s) 776, 1057, 1473, 1757
Vha464I CTTAAG 2 cut(s) 358, 1270
VpaK11BI GGWCC 3 cut(s) 611, 721, 766
VspI ATTAAT 1 cut(s) 1548
XapI RAATTY 6 cut(s) 12, 143, 191, 415, 911, 2190
XceI RCATGY 3 cut(s) 1564, 1615, 1951
XmaI CCCGGG 2 cut(s) 526, 932
XmaJI CCTAGG 1 cut(s) 977
XmiI GTMKAC 2 cut(s) 220, 389
XspI CTAG 8 cut(s) 275, 420, 569, 629, 675, 881, 978, 1677
ZrmI AGTACT 1 cut(s) 1279
Zsp2I ATGCAT 1 cut(s) 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.