MD12G1033500.v1.1

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000916
Physical Location & Seq
Reverse (-)
3700658 .. 3702967
2310 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1033500.v1.1.491

Sequence Viewer

Length: 2310 bp
ATGGATACGAGAATTCTACTCTGCTATACATTGAGATTTTTGCTATGTTGTACCTTCATTGCTTGTATTTCCACGGCGTCTCACACCATCTCTCCAGGCCAATCTCTCTCCGGGGAGCAAACAATCACCTCTCCAAGTGGCAAATTTGAGCTCGGTTTCTTCACACCAGCAATCACCTCTCCAAGCGGAAAATTTGAGCTCGGTTCCTTCGCACCAGGTATACCTCAAAACTATTACATAGGCATTTGGTACAAAAGTCTACCCAATCGGACTGTAGTTTGGGTCGCAAACAGAAAACAACCTGTTTGTGAACCGCATTTTTCAGTATTGCAACTTGTCGAAAATGGAAACTTGACCTTAAGTGGTCCCTCCAAAGTTGCAATATGGTCGACTAATTCAAGATCAAAGGTCTTTAATTCTAGTGTAGCAAAGCTTCTTGACAATGGGAACTTTGTCATAACGGATGCATTTAATTCATCTGTTGTCAAATGGCAGAGTTTCGATCACCCGACGGATACTTGGCTGCCGGGTGCTAAGCTTGGATACAATAATCGTACGAGGAGAAAACTAGTTCTTACATCCTGGAGAAATCCGAAAACTCCTGCACCTGGTCCTTTTTCTTCTGAGCTAGATGAAATACCTTTTAATTTATCTTCGTATCCTGATACCTTTACTAGAGGCAGACTTGAAATCAATGGTCAGCTAAAGTTCTATGCCTGGGACCAAGGCTTTACGGAATGGACTTTGATTTCGGTTGAACCATCGGACCAGTGTGCGGTTGATTCTTCATGCGGTGCTTTTAGTATTTGCAACCAACGGAGTTTTCCTCGTTGTGGTTGCCTCGAAGGATTTGAACCCGGAGTCAGGGAAAGTTGGATACTAGAGGACTTCTCAGATGGCTGTGTGAGGAAAATTCCTTTTCACTACAGTCCCAGGGACTCATTTTTGGCGATAACTGATGTTGGAATTGGATATCCTAAGAACTATGAGCAAATTATAAATGTGAGCAATGATCAATGCAGATTAGAATGCTTAATAAATTGTTCATGCAGTGCCTTTGTTTATAATTTTATCGATAGTCGTCGGTGTTTCATTTGGATAGGGGATCTATATAATATACTGACAATGCCATCCGAATCCATTTCCATAAGTTATTCTCCAGATAGCCCAGGATTGCATCTCCGGATTGTGGATTCTACAAGTGAGACAAAGAGAAAGACTACTTGGACTGTAATTGGATTACTTGCAGGGTTCTTTTCCATCTTAAGTAGTACTTTCATTGTCATGGTATTTTTTAGAAGGAGATGGTCAGCAGGAGCATTGGCAACTACCGACGATTCTTTGGTGCTTTACAAGCATAGAGATTTAAGAAGAGCAACAAACAACTTCTCCGAAAAACTTGGGGAAGGAGGCTTTGGTTCTGTATTCAAGGGGACGTTGCCTAACTCAACGGCCATAGCAGTGAAAGAGCTAAAAAGTATGAACCAGGGAGAGAAACAATTTCGTGCTGAAGTGGGAACTATCGGTGTAATCCAACACATTAATCTCATTCGCATGAGGGGATTCTGCGTGAACGCTTCAAAGAGATTTTTAGTTTATGACTACATGCCAAATGGTTCTCTGCAATCTCTTATTCTTGGAAAGAACCCGATAATGTTAGATTGGAATGCTAGATATCACATTGCAGTAGGGACTGCCAGAGGATTGTCTTACCTCCATGAAGAGTGCAGAGACTGCATAATACACTGCGATGTTAAGCCAGAGAACATCTTGTTGGATTCAGAATACAGTCCAAAATTGGCGGATTTTGGTCTTGCGAAACTCTTGGGGAGACACCACAGCCGGGTTTTGACAACCATGAGAGGGACTGTGGGCTATCTCGCACCAGAATGGTTTTCGGGTGAAGCCATCACCCCAAAAGCCGATGTGTTTAGCTATGGCATGTTGCTGATTGAGGTCATATCAGGGAGGAGAAACAGAGAAGGGTTAGCTGACGGGGTAGAAAACTTCCATCCTATCCGGCTAGCGAATGCAGTTAATAGAGGGGAAGATCTTTTCACCTTGTTGGATTGCAGGTTGGAAGGCAAAGCTGACAGAGATGACCTAAGTAGAGCGTGCAAAGTGGCTTGTTGGTGCATTCAAGAAGATGAGAAGGATAGGCCAACAATGAGGCAGGTTGTTCAAATTCTTGAGGGAGTTTTGGACATCGGTGTTCCGCCAGTCCCGCAGTTCCTCCAGCGTCTTATCAGAAGTCCGGCAGAAGGCATTAACTACCAGGAGACTACATCCGATTCAGGTTCATGGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_2 Pkinase S_locus_glycop

Protein Analysis

770

Amino Acids

86.07

Weight (kDa)

7.71

Isoelectric Point (pI)

46.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 91 - 196 2.6e-33 D-mannose binding lectin
S_locus_glycop PF00954 222 - 286 3.6e-14 S-locus glycoprotein domain
PAN_2 PF08276 312 - 373 4.2e-12 PAN-like domain
PK_Tyr_Ser-Thr PF07714 463 - 730 4.5e-40 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 464 - 728 2.4e-48 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 998, 1065
Acc36I ACCTGC 2 cut(s) 2064, 2164
AccI GTMKAC 3 cut(s) 220, 259, 389
AccIII TCCGGA 1 cut(s) 1182
AciI CCGC 7 cut(s) 186, 314, 776, 792, 1802, 2216, 2225
AclWI GGATC 1 cut(s) 1113
AcoI YGGCCR 1 cut(s) 1452
AcsI RAATTY 5 cut(s) 12, 143, 191, 912, 2184
AcuI CTGAAG 1 cut(s) 1530
AcyI GRCGYC 1 cut(s) 77
AfaI GTAC 4 cut(s) 52, 251, 556, 1273
AfiI CCNNNNNNNGG 8 cut(s) 608, 775, 833, 864, 1189, 1843, 1863, 2261
AflII CTTAAG 2 cut(s) 358, 1264
AgsI TTSAA 8 cut(s) 399, 689, 758, 854, 1429, 1581, 2141, 2183
AhlI ACTAGT 1 cut(s) 568
AjnI CCWGG 9 cut(s) 94, 214, 581, 607, 716, 932, 1168, 1485, 2274
AjuI GAANNNNNNNTTGG 8 cut(s) 807, 839, 1207, 1239, 1398, 1430, 1757, 1789
Alw21I GWGCWC 2 cut(s) 153, 201
Alw26I GTCTC 5 cut(s) 84, 1199, 1725, 1825, 2273
AlwI GGATC 1 cut(s) 1113
AlwNI CAGNNNCTG 1 cut(s) 1734
Aor13HI TCCGGA 1 cut(s) 1182
AoxI GGCC 3 cut(s) 97, 1452, 2159
ApeKI GCWGC 1 cut(s) 523
ApoI RAATTY 5 cut(s) 12, 143, 191, 912, 2184
ArsI GACNNNNNNTTYG 2 cut(s) 733, 765
AseI ATTAAT 1 cut(s) 1542
AspS9I GGNCC 4 cut(s) 365, 611, 721, 766
AsuC2I CCSGG 4 cut(s) 112, 528, 858, 1844
AsuHPI GGTGA 6 cut(s) 118, 166, 497, 1903, 1913, 2050
AsuNHI GCTAGC 1 cut(s) 2023
AvaII GGWCC 4 cut(s) 365, 611, 721, 766
BaeI ACNNNNGTAYC 2 cut(s) 241, 274
BanII GRGCYC 2 cut(s) 153, 201
BarI GAAGNNNNNNTAC 2 cut(s) 417, 449
Bbv12I GWGCWC 2 cut(s) 153, 201
BbvI GCAGC 1 cut(s) 510
BccI CCATC 8 cut(s) 95, 769, 890, 1138, 1268, 1299, 1916, 2019
BceAI ACGGC 2 cut(s) 90, 1467
BcgI CGANNNNNNTGC 2 cut(s) 369, 403
BciT130I CCWGG 9 cut(s) 96, 216, 583, 609, 718, 934, 1170, 1487, 2276
BciVI GTATCC 4 cut(s) 508, 536, 669, 870
BclI TGATCA 1 cut(s) 1012
BcnI CCSGG 4 cut(s) 112, 528, 858, 1844
BcoDI GTCTC 5 cut(s) 84, 1199, 1725, 1825, 2273
BcuI ACTAGT 1 cut(s) 568
BfaI CTAG 7 cut(s) 420, 569, 629, 675, 881, 1671, 2024
BfmI CTRYAG 2 cut(s) 273, 925
BfrI CTTAAG 2 cut(s) 358, 1264
BfuAI ACCTGC 2 cut(s) 2064, 2164
BfuI GTATCC 4 cut(s) 508, 536, 669, 870
BglII AGATCT 1 cut(s) 2050
BisI GCNGC 1 cut(s) 524
BlpI GCTNAGC 1 cut(s) 534
BlsI GCNGC 1 cut(s) 525
BmcAI AGTACT 1 cut(s) 1273
Bme18I GGWCC 4 cut(s) 365, 611, 721, 766
BmgT120I GGNCC 4 cut(s) 365, 611, 721, 766
BmiI GGNNCC 3 cut(s) 205, 367, 722
BmsI GCATC 2 cut(s) 454, 1186
BmtI GCTAGC 1 cut(s) 2027
BplI GAGNNNNNCTC 4 cut(s) 811, 843, 875, 907
BpmI CTGGAG 4 cut(s) 78, 604, 1143, 2219
Bpu1102I GCTNAGC 1 cut(s) 534
BpuEI CTTGAG 1 cut(s) 2210
BpuMI CCSGG 4 cut(s) 112, 528, 858, 1844
Bsa29I ATCGAT 1 cut(s) 1074
BsaHI GRCGYC 1 cut(s) 77
BsaJI CCNNGG 8 cut(s) 72, 111, 717, 724, 932, 933, 1168, 1486
BsaWI WCCGGW 1 cut(s) 1182
BsaXI ACNNNNNCTCC 4 cut(s) 76, 106, 1373, 1403
Bsc4I CCNNNNNNNGG 8 cut(s) 608, 775, 833, 864, 1189, 1843, 1863, 2261
Bse1I ACTGG 2 cut(s) 769, 2219
Bse3DI GCAATG 3 cut(s) 57, 1015, 1680
BseAI TCCGGA 1 cut(s) 1182
BseBI CCWGG 9 cut(s) 96, 216, 583, 609, 718, 934, 1170, 1487, 2276
BseCI ATCGAT 1 cut(s) 1074
BseDI CCNNGG 8 cut(s) 72, 111, 717, 724, 932, 933, 1168, 1486
BseGI GGATG 5 cut(s) 469, 578, 1130, 2011, 2285
BseLI CCNNNNNNNGG 8 cut(s) 608, 775, 833, 864, 1189, 1843, 1863, 2261
BseMI GCAATG 3 cut(s) 57, 1015, 1680
BseMII CTCAG 2 cut(s) 615, 906
BseNI ACTGG 2 cut(s) 769, 2219
BseRI GAGGAG 2 cut(s) 574, 1984
BseXI GCAGC 1 cut(s) 510
BsgI GTGCAG 2 cut(s) 588, 1747
BshFI GGCC 3 cut(s) 99, 1454, 2161
BshVI ATCGAT 1 cut(s) 1074
BsiHKAI GWGCWC 2 cut(s) 153, 201
BsiSI CCGG 7 cut(s) 111, 527, 858, 1183, 1843, 2020, 2255
BsiWI CGTACG 1 cut(s) 554
BslFI GGGAC 8 cut(s) 351, 734, 915, 950, 1447, 1705, 1879, 2207
BslI CCNNNNNNNGG 8 cut(s) 608, 775, 833, 864, 1189, 1843, 1863, 2261
BsmAI GTCTC 5 cut(s) 84, 1199, 1725, 1825, 2273
BsmBI CGTCTC 1 cut(s) 84
BsmFI GGGAC 8 cut(s) 351, 734, 915, 950, 1447, 1705, 1879, 2207
BsmI GAATGC 4 cut(s) 1034, 1672, 2035, 2136
BsnI GGCC 3 cut(s) 99, 1454, 2161
Bsp1286I GDGCHC 2 cut(s) 153, 201
Bsp13I TCCGGA 1 cut(s) 1182
Bsp143I GATC 5 cut(s) 401, 502, 1012, 1105, 2050
Bsp1720I GCTNAGC 1 cut(s) 534
BspACI CCGC 7 cut(s) 186, 314, 776, 792, 1802, 2216, 2225
BspANI GGCC 3 cut(s) 99, 1454, 2161
BspCNI CTCAG 2 cut(s) 616, 905
BspDI ATCGAT 1 cut(s) 1074
BspEI TCCGGA 1 cut(s) 1182
BspHI TCATGA 1 cut(s) 2306
BspLI GGNNCC 3 cut(s) 205, 367, 722
BspMI ACCTGC 2 cut(s) 2064, 2164
BspOI GCTAGC 1 cut(s) 2027
BspPI GGATC 1 cut(s) 1113
BspQI GCTCTTC 1 cut(s) 1366
BspTI CTTAAG 2 cut(s) 358, 1264
BsrDI GCAATG 3 cut(s) 57, 1015, 1680
BsrI ACTGG 2 cut(s) 769, 2219
BssECI CCNNGG 8 cut(s) 72, 111, 717, 724, 932, 933, 1168, 1486
BssMI GATC 5 cut(s) 401, 502, 1012, 1105, 2050
BssNAI GTATAC 1 cut(s) 221
BssNI GRCGYC 1 cut(s) 77
BssT1I CCWWGG 1 cut(s) 724
Bst1107I GTATAC 1 cut(s) 221
Bst2UI CCWGG 9 cut(s) 96, 216, 583, 609, 718, 934, 1170, 1487, 2276
Bst4CI ACNGT 5 cut(s) 274, 929, 1231, 1790, 1870
Bst6I CTCTTC 2 cut(s) 1366, 1716
BstACI GRCGYC 1 cut(s) 77
BstAFI CTTAAG 2 cut(s) 358, 1264
BstAPI GCANNNNNTGC 1 cut(s) 1734
BstC8I GCNNGC 2 cut(s) 2025, 2116
BstDEI CTNAG 5 cut(s) 534, 624, 892, 978, 2105
BstDSI CCRYGG 1 cut(s) 72
BstF5I GGATG 5 cut(s) 469, 578, 1130, 2011, 2285
BstKTI GATC 5 cut(s) 404, 505, 1015, 1108, 2053
BstMAI GTCTC 5 cut(s) 84, 1199, 1725, 1825, 2273
BstMBI GATC 5 cut(s) 401, 502, 1012, 1105, 2050
BstMWI GCNNNNNNNGC 3 cut(s) 1354, 1734, 2224
BstNI CCWGG 9 cut(s) 96, 216, 583, 609, 718, 934, 1170, 1487, 2276
BstNSI RCATGY 2 cut(s) 1609, 1945
BstSFI CTRYAG 2 cut(s) 273, 925
BstV1I GCAGC 1 cut(s) 510
BstX2I RGATCY 2 cut(s) 1105, 2050
BstYI RGATCY 2 cut(s) 1105, 2050
BstZ17I GTATAC 1 cut(s) 221
Bsu15I ATCGAT 1 cut(s) 1074
BsuI GTATCC 4 cut(s) 508, 536, 669, 870
BsuRI GGCC 3 cut(s) 99, 1454, 2161
BsuTUI ATCGAT 1 cut(s) 1074
BtgI CCRYGG 1 cut(s) 72
BtgZI GCGATG 1 cut(s) 1764
BtsCI GGATG 5 cut(s) 469, 578, 1130, 2011, 2285
BtsI GCAGTG 3 cut(s) 1057, 1467, 1744
BtsIMutI CAGTG 4 cut(s) 776, 1057, 1467, 1744
BveI ACCTGC 2 cut(s) 2064, 2164
Cac8I GCNNGC 2 cut(s) 2025, 2116
CaiI CAGNNNCTG 1 cut(s) 1734
CciI TCATGA 1 cut(s) 2306
Cfr13I GGNCC 4 cut(s) 365, 611, 721, 766
ClaI ATCGAT 1 cut(s) 1074
CseI GACGC 2 cut(s) 66, 2228
CsiI ACCWGGT 2 cut(s) 214, 607
Csp6I GTAC 4 cut(s) 51, 250, 555, 1272
CviQI GTAC 4 cut(s) 51, 250, 555, 1272
DdeI CTNAG 5 cut(s) 534, 624, 892, 978, 2105
DpnI GATC 5 cut(s) 403, 504, 1014, 1107, 2052
DpnII GATC 5 cut(s) 401, 502, 1012, 1105, 2050
EaeI YGGCCR 1 cut(s) 1452
Eam1104I CTCTTC 2 cut(s) 1366, 1716
EarI CTCTTC 2 cut(s) 1366, 1716
EciI GGCGGA 2 cut(s) 1817, 2205
Ecl136II GAGCTC 2 cut(s) 151, 199
Eco130I CCWWGG 1 cut(s) 724
Eco24I GRGCYC 2 cut(s) 153, 201
Eco32I GATATC 2 cut(s) 974, 1676
Eco47I GGWCC 4 cut(s) 365, 611, 721, 766
Eco53kI GAGCTC 2 cut(s) 151, 199
Eco57I CTGAAG 1 cut(s) 1530
EcoICRI GAGCTC 2 cut(s) 151, 199
EcoRI GAATTC 1 cut(s) 12
EcoRII CCWGG 9 cut(s) 94, 214, 581, 607, 716, 932, 1168, 1485, 2274
EcoRV GATATC 2 cut(s) 974, 1676
EcoT14I CCWWGG 1 cut(s) 724
EcoT22I ATGCAT 1 cut(s) 469
EcoT38I GRGCYC 2 cut(s) 153, 201
ErhI CCWWGG 1 cut(s) 724
Esp3I CGTCTC 1 cut(s) 84
FalI AAGNNNNNCTT 2 cut(s) 1258, 1290
FaqI GGGAC 8 cut(s) 351, 734, 915, 950, 1447, 1705, 1879, 2207
FauI CCCGC 1 cut(s) 2232
FbaI TGATCA 1 cut(s) 1012
FblI GTMKAC 3 cut(s) 220, 259, 389
Fnu4HI GCNGC 1 cut(s) 524
FokI GGATG 5 cut(s) 476, 565, 1117, 1998, 2272
FriOI GRGCYC 2 cut(s) 153, 201
Fsp4HI GCNGC 1 cut(s) 524
FspBI CTAG 7 cut(s) 420, 569, 629, 675, 881, 1671, 2024
GluI GCNGC 1 cut(s) 524
GsuI CTGGAG 4 cut(s) 78, 604, 1143, 2219
HaeIII GGCC 3 cut(s) 99, 1454, 2161
HapII CCGG 7 cut(s) 111, 527, 858, 1183, 1843, 2020, 2255
HgaI GACGC 2 cut(s) 66, 2228
Hin1I GRCGYC 1 cut(s) 77
HincII GTYRAC 1 cut(s) 390
HindII GTYRAC 1 cut(s) 390
HindIII AAGCTT 2 cut(s) 431, 536
HinfI GANTC 9 cut(s) 782, 861, 938, 1136, 1193, 1337, 1563, 1778, 2291
HpaII CCGG 7 cut(s) 111, 527, 858, 1183, 1843, 2020, 2255
HphI GGTGA 6 cut(s) 118, 166, 497, 1903, 1913, 2050
Hpy166II GTNNAC 5 cut(s) 221, 260, 311, 390, 1573
Hpy188III TCNNGA 8 cut(s) 399, 437, 662, 1160, 1183, 2141, 2189, 2307
Hpy8I GTNNAC 5 cut(s) 221, 260, 311, 390, 1573
Hpy99I CGWCG 3 cut(s) 514, 1086, 1337
HpyAV CCTTC 9 cut(s) 64, 217, 839, 1293, 1400, 1976, 2075, 2146, 2255
HpyCH4III ACNGT 5 cut(s) 274, 929, 1231, 1790, 1870
HpyCH4IV ACGT 1 cut(s) 1436
HpyF10VI GCNNNNNNNGC 3 cut(s) 1354, 1734, 2224
HpyF3I CTNAG 5 cut(s) 534, 624, 892, 978, 2105
HpySE526I ACGT 1 cut(s) 1436
Hsp92I GRCGYC 1 cut(s) 77
Kpn2I TCCGGA 1 cut(s) 1182
Ksp22I TGATCA 1 cut(s) 1012
Kzo9I GATC 5 cut(s) 401, 502, 1012, 1105, 2050
LguI GCTCTTC 1 cut(s) 1366
LmnI GCTCC 2 cut(s) 115, 1316
Lsp1109I GCAGC 1 cut(s) 510
LweI GCATC 2 cut(s) 454, 1186
MabI ACCWGGT 2 cut(s) 214, 607
MaeI CTAG 7 cut(s) 420, 569, 629, 675, 881, 1671, 2024
MaeII ACGT 1 cut(s) 1436
MalI GATC 5 cut(s) 403, 504, 1014, 1107, 2052
MboI GATC 5 cut(s) 401, 502, 1012, 1105, 2050
MboII GAAGA 8 cut(s) 151, 612, 645, 777, 1383, 1733, 2060, 2156
MflI RGATCY 2 cut(s) 1105, 2050
MhlI GDGCHC 2 cut(s) 153, 201
MlyI GAGTC 2 cut(s) 870, 932
MmeI TCCRAC 6 cut(s) 854, 943, 1558, 1755, 2046, 2058
Mph1103I ATGCAT 1 cut(s) 469
MroI TCCGGA 1 cut(s) 1182
MslI CAYNNNNRTG 6 cut(s) 1283, 1460, 1553, 1749, 1888, 2305
MspCI CTTAAG 2 cut(s) 358, 1264
MspI CCGG 7 cut(s) 111, 527, 858, 1183, 1843, 2020, 2255
Mva1269I GAATGC 4 cut(s) 1034, 1672, 2035, 2136
MvaI CCWGG 9 cut(s) 96, 216, 583, 609, 718, 934, 1170, 1487, 2276
MwoI GCNNNNNNNGC 3 cut(s) 1354, 1734, 2224
NciI CCSGG 4 cut(s) 112, 528, 858, 1844
NdeII GATC 5 cut(s) 401, 502, 1012, 1105, 2050
NheI GCTAGC 1 cut(s) 2023
NlaIV GGNNCC 3 cut(s) 205, 367, 722
NsiI ATGCAT 1 cut(s) 469
NspI RCATGY 2 cut(s) 1609, 1945
PagI TCATGA 1 cut(s) 2306
PasI CCCWGGG 1 cut(s) 933
PciSI GCTCTTC 1 cut(s) 1366
PctI GAATGC 4 cut(s) 1034, 1672, 2035, 2136
PfeI GAWTC 7 cut(s) 782, 1136, 1193, 1337, 1563, 1778, 2291
Pfl23II CGTACG 1 cut(s) 554
PfoI TCCNGGA 1 cut(s) 581
PkrI GCNGC 1 cut(s) 525
PleI GAGTC 2 cut(s) 869, 932
PpsI GAGTC 2 cut(s) 869, 932
PshBI ATTAAT 1 cut(s) 1542
PsiI TTATAA 2 cut(s) 998, 1065
Psp124BI GAGCTC 2 cut(s) 153, 201
Psp6I CCWGG 9 cut(s) 94, 214, 581, 607, 716, 932, 1168, 1485, 2274
PspGI CCWGG 9 cut(s) 94, 214, 581, 607, 716, 932, 1168, 1485, 2274
PspLI CGTACG 1 cut(s) 554
PspN4I GGNNCC 3 cut(s) 205, 367, 722
PspPI GGNCC 4 cut(s) 365, 611, 721, 766
PstNI CAGNNNCTG 1 cut(s) 1734
PsuI RGATCY 2 cut(s) 1105, 2050
RsaI GTAC 4 cut(s) 52, 251, 556, 1273
RsaNI GTAC 4 cut(s) 51, 250, 555, 1272
RseI CAYNNNNRTG 6 cut(s) 1283, 1460, 1553, 1749, 1888, 2305
SacI GAGCTC 2 cut(s) 153, 201
SalI GTCGAC 1 cut(s) 388
SapI GCTCTTC 1 cut(s) 1366
SatI GCNGC 1 cut(s) 524
Sau3AI GATC 5 cut(s) 401, 502, 1012, 1105, 2050
Sau96I GGNCC 4 cut(s) 365, 611, 721, 766
ScaI AGTACT 1 cut(s) 1273
SchI GAGTC 2 cut(s) 870, 932
SduI GDGCHC 2 cut(s) 153, 201
SexAI ACCWGGT 2 cut(s) 214, 607
SfaNI GCATC 2 cut(s) 454, 1186
SfcI CTRYAG 2 cut(s) 273, 925
SinI GGWCC 4 cut(s) 365, 611, 721, 766
SmiMI CAYNNNNRTG 6 cut(s) 1283, 1460, 1553, 1749, 1888, 2305
SmlI CTYRAG 3 cut(s) 358, 1264, 2189
SmoI CTYRAG 3 cut(s) 358, 1264, 2189
SpeI ACTAGT 1 cut(s) 568
SsiI CCGC 7 cut(s) 186, 314, 776, 792, 1802, 2216, 2225
SspMI CTAG 7 cut(s) 420, 569, 629, 675, 881, 1671, 2024
SstI GAGCTC 2 cut(s) 153, 201
StyI CCWWGG 1 cut(s) 724
TaaI ACNGT 5 cut(s) 274, 929, 1231, 1790, 1870
TaiI ACGT 1 cut(s) 1439
TaqI TCGA 5 cut(s) 339, 389, 501, 843, 1074
TatI WGTACW 1 cut(s) 1271
TfiI GAWTC 7 cut(s) 782, 1136, 1193, 1337, 1563, 1778, 2291
TscAI CASTG 4 cut(s) 776, 1057, 1467, 1751
TseI GCWGC 1 cut(s) 523
TspGWI ACGGA 4 cut(s) 476, 527, 749, 832
TspRI CASTG 4 cut(s) 776, 1057, 1467, 1751
Vha464I CTTAAG 2 cut(s) 358, 1264
VpaK11BI GGWCC 4 cut(s) 365, 611, 721, 766
VspI ATTAAT 1 cut(s) 1542
XapI RAATTY 5 cut(s) 12, 143, 191, 912, 2184
XceI RCATGY 2 cut(s) 1609, 1945
XmiI GTMKAC 3 cut(s) 220, 259, 389
XspI CTAG 7 cut(s) 420, 569, 629, 675, 881, 1671, 2024
ZrmI AGTACT 1 cut(s) 1273
Zsp2I ATGCAT 1 cut(s) 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.