MD17G1108200.v1.1

G-type lectin S-receptor-like serine threonine-protein kinase At2g19130

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000916
Physical Location & Seq
Reverse (-)
9288333 .. 9290096
1764 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1108200.v1.1.491

Sequence Viewer

Length: 1485 bp
ATGGATATCAGCCAATCTCTTTGTGGAAGCCAAACACTCACCTCTCCAGGTGACAGATTTGAGCTGGGTTTCTTCACAACAGGTGACTCTCGAAACTATTACGTAGGCATTTGGTACAAAAATCTCCGCCCTCGAACAGTAGTTTGGGTAACAAACAGAAAACAACCTGCTTCTGATTCATATTCTTCGGCATTGCAACTCTTCCGAAATGGAAGCTTAGAGTTGCTGGACCGAACCAAATATACAATTTGGTCAACTCGTTCTGCGGCAGCGGTTTCTAATTCCACCATAGCAGTGCTTCTTGACAACGGGAACTTCGTCATCAGTGATGAATCTGATTCATCCGCCGTTTTATGGCAGAGTTTTGATCACCCCACTGACACTTGGCTTCCAGGTGCCAAGCTTGGATATAATAAGCTTACCAAGGAAAAGCTAATCCTTACTCCCTGGAGAAACCCACAAAATCCAGCCCCTGGAATATTTTCACTTGAGATAGAACAAGCTGGAAGCAGGGTTTTCTTATTTCGAAATGGTTCCGCCATTAGTATTTGGGATTGGCAAGGTGACAATCACTACTGCTTTTATTGTCAAGTGAATTTGAATTCTGATGTAAATGAGAATGAAAGCTCTTTAACTTATGTTCCCATCGATCCTAATTCATTTGTGAGATATAAACTTTTGAATGGTCTACTCAAGTTTTATAACGGCAGTGAGACAATAGACACTTGGGGTGCTCTTGGACAATGTACTAACGATGTTTCGTGTGGTGCTTTTAGTATCTGTGACTTTCAGAATTTGCCTCATTGTCGATGCTTGGAAGGATTTGAACCCAAAATCCCAGAAGATTGGGAATTTGGAGGTTATTCTTATGGTTGTGTGAGGAAAACTCCTTTGCAGTGCAGTGATGTAGGAAATCACAAATTTTTGGGAGTACCTGATGTTTCTTATTCCCAAAATTCTGAATCGTTAAAGTTTAAAAGCATTGAGGAATGTAGATTAGCATGCTCAAGAGATTGCTCGATCACTGCCTTCGCTTACAATAACGACTGTATGATTTGGAAAGGGGATGTATTCGATCTGTATCAACTACCATACTCTGTAACTAAACTAATGGATGGCATAAAAATTCATATACGAGTTGCAGATTCTACCAACAAGGTTAAGAGAAAGACTACTTGGATTGCTATTGTAGGGATATTGCCCAATTCAACTGCCATAGCAGTGAAAGAACTTAAATATCTGGAGCAGGGAGAGAAGCAATTTCACGCTGAAGTGGGGACAATTAGCACAATCCACCACATTAATCTTGTTCGCCTTTGGGGATTTTGCGCAGAAACTTCAAAACGACTTTTGGTATATGAGTACATGCCAAATGGTTCTCTGCAATCTCTTTTTCATCAAGAGAATGCACTAATGTTAGATTGGAAAGACAGATATCATATTGCCATGGGGATTGCCAGAGGATTGGCTTACCTACATGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_2 Pkinase S_locus_glycop

Protein Analysis

495

Amino Acids

55.94

Weight (kDa)

6.15

Isoelectric Point (pI)

38.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 46 - 151 9.3e-34 D-mannose binding lectin
S_locus_glycop PF00954 195 - 278 2.6e-08 S-locus glycoprotein domain
PAN_2 PF08276 303 - 360 3.3e-09 PAN-like domain
PK_Tyr_Ser-Thr PF07714 402 - 494 6e-15 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 404 - 494 6.1e-11 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 702
Acc16I TGCGCA 1 cut(s) 1330
Acc36I ACCTGC 1 cut(s) 175
AccB1I GGYRCC 1 cut(s) 395
AccB7I CCANNNNNTGG 1 cut(s) 473
AccI GTMKAC 1 cut(s) 688
AciI CCGC 5 cut(s) 127, 266, 272, 345, 537
AclWI GGATC 1 cut(s) 644
AcsI RAATTY 7 cut(s) 595, 601, 793, 851, 920, 955, 1125
AcuI CTGAAG 1 cut(s) 1290
AfaI GTAC 4 cut(s) 116, 748, 933, 1364
AfiI CCNNNNNNNGG 2 cut(s) 354, 473
AgsI TTSAA 5 cut(s) 601, 682, 827, 1209, 1341
AjnI CCWGG 4 cut(s) 46, 391, 446, 472
AjuI GAANNNNNNNTTGG 4 cut(s) 127, 159, 1159, 1191
AloI GAACNNNNNNTCC 2 cut(s) 819, 851
AluBI AGCT 7 cut(s) 64, 216, 403, 418, 433, 503, 627
AluI AGCT 7 cut(s) 64, 216, 403, 418, 433, 503, 627
Alw21I GWGCWC 1 cut(s) 736
Alw26I GTCTC 1 cut(s) 707
AlwI GGATC 1 cut(s) 644
AlwNI CAGNNNCTG 1 cut(s) 473
ApeKI GCWGC 1 cut(s) 269
ApoI RAATTY 7 cut(s) 595, 601, 793, 851, 920, 955, 1125
AseI ATTAAT 1 cut(s) 1302
Asp700I GAANNNNTTC 2 cut(s) 481, 532
AspLEI GCGC 1 cut(s) 1331
AspS9I GGNCC 1 cut(s) 229
AsuHPI GGTGA 5 cut(s) 31, 62, 95, 362, 575
AsuII TTCGAA 1 cut(s) 526
AvaII GGWCC 1 cut(s) 229
BanI GGYRCC 1 cut(s) 395
Bbv12I GWGCWC 1 cut(s) 736
BbvI GCAGC 1 cut(s) 281
BccI CCATC 2 cut(s) 653, 1109
BceAI ACGGC 2 cut(s) 332, 721
BciT130I CCWGG 4 cut(s) 48, 393, 448, 474
BclI TGATCA 1 cut(s) 367
BcoDI GTCTC 1 cut(s) 707
BfuAI ACCTGC 1 cut(s) 175
BisI GCNGC 2 cut(s) 267, 270
BlsI GCNGC 2 cut(s) 268, 271
Bme1390I CCNGG 4 cut(s) 48, 393, 448, 474
Bme18I GGWCC 1 cut(s) 229
BmgT120I GGNCC 1 cut(s) 229
BmiI GGNNCC 2 cut(s) 397, 535
BmrFI CCNGG 4 cut(s) 48, 393, 448, 474
BmsI GCATC 1 cut(s) 800
BplI GAGNNNNNCTC 2 cut(s) 871, 903
BpmI CTGGAG 3 cut(s) 30, 469, 1262
Bpu14I TTCGAA 1 cut(s) 526
BpuEI CTTGAG 3 cut(s) 509, 677, 991
Bsa29I ATCGAT 1 cut(s) 648
BsaAI YACGTR 1 cut(s) 103
BsaBI GATNNNNATC 1 cut(s) 1080
BsaJI CCNNGG 4 cut(s) 423, 446, 472, 1446
Bsc4I CCNNNNNNNGG 2 cut(s) 354, 473
Bse3DI GCAATG 1 cut(s) 191
Bse8I GATNNNNATC 1 cut(s) 1080
BseBI CCWGG 4 cut(s) 48, 393, 448, 474
BseCI ATCGAT 1 cut(s) 648
BseDI CCNNGG 4 cut(s) 423, 446, 472, 1446
BseGI GGATG 3 cut(s) 341, 1072, 1120
BseJI GATNNNNATC 1 cut(s) 1080
BseLI CCNNNNNNNGG 2 cut(s) 354, 473
BseMI GCAATG 1 cut(s) 191
BseXI GCAGC 1 cut(s) 281
BseYI CCCAGC 1 cut(s) 64
BsgI GTGCAG 1 cut(s) 919
BshNI GGYRCC 1 cut(s) 395
BshVI ATCGAT 1 cut(s) 648
BsiHKAI GWGCWC 1 cut(s) 736
BslFI GGGAC 1 cut(s) 1291
BslI CCNNNNNNNGG 2 cut(s) 354, 473
BsmAI GTCTC 1 cut(s) 707
BsmFI GGGAC 1 cut(s) 1291
BsmI GAATGC 1 cut(s) 1411
Bsp119I TTCGAA 1 cut(s) 526
Bsp1286I GDGCHC 1 cut(s) 736
Bsp143I GATC 4 cut(s) 367, 649, 1020, 1075
Bsp19I CCATGG 1 cut(s) 1446
BspACI CCGC 5 cut(s) 127, 266, 272, 345, 537
BspDI ATCGAT 1 cut(s) 648
BspLI GGNNCC 2 cut(s) 397, 535
BspMI ACCTGC 1 cut(s) 175
BspPI GGATC 1 cut(s) 644
BspT104I TTCGAA 1 cut(s) 526
BspT107I GGYRCC 1 cut(s) 395
BsrDI GCAATG 1 cut(s) 191
BssECI CCNNGG 4 cut(s) 423, 446, 472, 1446
BssMI GATC 4 cut(s) 367, 649, 1020, 1075
BssT1I CCWWGG 2 cut(s) 423, 1446
Bst2UI CCWGG 4 cut(s) 48, 393, 448, 474
Bst4CI ACNGT 2 cut(s) 139, 1049
Bst6I CTCTTC 1 cut(s) 206
BstBAI YACGTR 1 cut(s) 103
BstBI TTCGAA 1 cut(s) 526
BstC8I GCNNGC 1 cut(s) 1003
BstDEI CTNAG 1 cut(s) 217
BstDSI CCRYGG 1 cut(s) 1446
BstF5I GGATG 3 cut(s) 341, 1072, 1120
BstHHI GCGC 1 cut(s) 1331
BstKTI GATC 4 cut(s) 370, 652, 1023, 1078
BstMAI GTCTC 1 cut(s) 707
BstMBI GATC 4 cut(s) 367, 649, 1020, 1075
BstNI CCWGG 4 cut(s) 48, 393, 448, 474
BstNSI RCATGY 2 cut(s) 1005, 1369
BstSCI CCNGG 4 cut(s) 46, 391, 446, 472
BstSNI TACGTA 1 cut(s) 103
BstV1I GCAGC 1 cut(s) 281
BstXI CCANNNNNNTGG 2 cut(s) 846, 1465
Bsu15I ATCGAT 1 cut(s) 648
BsuTUI ATCGAT 1 cut(s) 648
BtgI CCRYGG 1 cut(s) 1446
BtsCI GGATG 3 cut(s) 341, 1072, 1120
BtsI GCAGTG 6 cut(s) 300, 715, 902, 907, 1023, 1227
BtsIMutI CAGTG 8 cut(s) 300, 331, 375, 715, 902, 907, 1023, 1227
BveI ACCTGC 1 cut(s) 175
Cac8I GCNNGC 1 cut(s) 1003
CaiI CAGNNNCTG 1 cut(s) 473
CfoI GCGC 1 cut(s) 1331
Cfr13I GGNCC 1 cut(s) 229
ClaI ATCGAT 1 cut(s) 648
Csp6I GTAC 4 cut(s) 115, 747, 932, 1363
CviAII CATG 4 cut(s) 1002, 1366, 1447, 1478
CviQI GTAC 4 cut(s) 115, 747, 932, 1363
DdeI CTNAG 1 cut(s) 217
DpnI GATC 4 cut(s) 369, 651, 1022, 1077
DpnII GATC 4 cut(s) 367, 649, 1020, 1075
DraI TTTAAA 1 cut(s) 976
Eam1104I CTCTTC 1 cut(s) 206
EarI CTCTTC 1 cut(s) 206
EciI GGCGGA 3 cut(s) 116, 334, 526
Eco105I TACGTA 1 cut(s) 103
Eco130I CCWWGG 2 cut(s) 423, 1446
Eco32I GATATC 2 cut(s) 7, 1436
Eco47I GGWCC 1 cut(s) 229
Eco57I CTGAAG 1 cut(s) 1290
EcoRI GAATTC 1 cut(s) 601
EcoRII CCWGG 4 cut(s) 46, 391, 446, 472
EcoRV GATATC 2 cut(s) 7, 1436
EcoT14I CCWWGG 2 cut(s) 423, 1446
ErhI CCWWGG 2 cut(s) 423, 1446
FaeI CATG 4 cut(s) 1005, 1369, 1450, 1481
FaqI GGGAC 1 cut(s) 1291
FatI CATG 4 cut(s) 1001, 1365, 1446, 1477
FbaI TGATCA 1 cut(s) 367
FblI GTMKAC 1 cut(s) 688
Fnu4HI GCNGC 2 cut(s) 267, 270
FokI GGATG 3 cut(s) 328, 1079, 1127
Fsp4HI GCNGC 2 cut(s) 267, 270
FspI TGCGCA 1 cut(s) 1330
GlaI GCGC 1 cut(s) 1330
GluI GCNGC 2 cut(s) 267, 270
GsaI CCCAGC 1 cut(s) 68
GsuI CTGGAG 3 cut(s) 30, 469, 1262
HhaI GCGC 1 cut(s) 1331
Hin1II CATG 4 cut(s) 1005, 1369, 1450, 1481
Hin6I GCGC 1 cut(s) 1329
HinP1I GCGC 1 cut(s) 1329
HincII GTYRAC 1 cut(s) 255
HindII GTYRAC 1 cut(s) 255
HindIII AAGCTT 3 cut(s) 214, 401, 416
HinfI GANTC 6 cut(s) 86, 176, 332, 338, 962, 1145
HphI GGTGA 5 cut(s) 31, 62, 95, 362, 575
Hpy166II GTNNAC 2 cut(s) 255, 689
Hpy188I TCNGA 6 cut(s) 175, 206, 337, 607, 792, 961
Hpy188III TCNNGA 5 cut(s) 90, 302, 1008, 1241, 1400
Hpy8I GTNNAC 2 cut(s) 255, 689
HpyAV CCTTC 2 cut(s) 812, 1039
HpyCH4III ACNGT 2 cut(s) 139, 1049
HpyCH4IV ACGT 1 cut(s) 102
HpyCH4V TGCA 6 cut(s) 196, 895, 900, 1142, 1384, 1409
HpyF3I CTNAG 1 cut(s) 217
HpySE526I ACGT 1 cut(s) 102
Hsp92II CATG 4 cut(s) 1005, 1369, 1450, 1481
HspAI GCGC 1 cut(s) 1329
Ksp22I TGATCA 1 cut(s) 367
Kzo9I GATC 4 cut(s) 367, 649, 1020, 1075
LmnI GCTCC 1 cut(s) 1243
Lsp1109I GCAGC 1 cut(s) 281
LweI GCATC 1 cut(s) 800
MaeII ACGT 1 cut(s) 102
MaeIII GTNAC 6 cut(s) 50, 83, 148, 563, 782, 1099
MalI GATC 4 cut(s) 369, 651, 1022, 1077
MboI GATC 4 cut(s) 367, 649, 1020, 1075
MboII GAAGA 4 cut(s) 64, 177, 193, 854
MhlI GDGCHC 1 cut(s) 736
MlyI GAGTC 1 cut(s) 80
MnlI CCTC 7 cut(s) 52, 141, 810, 851, 873, 979, 1454
MroXI GAANNNNTTC 2 cut(s) 481, 532
MseI TTAA 6 cut(s) 632, 968, 975, 1161, 1233, 1302
MslI CAYNNNNRTG 2 cut(s) 293, 1220
MspA1I CMGCKG 1 cut(s) 272
MspR9I CCNGG 4 cut(s) 48, 393, 448, 474
Mva1269I GAATGC 1 cut(s) 1411
MvaI CCWGG 4 cut(s) 48, 393, 448, 474
NcoI CCATGG 1 cut(s) 1446
NdeII GATC 4 cut(s) 367, 649, 1020, 1075
NlaIII CATG 4 cut(s) 1005, 1369, 1450, 1481
NlaIV GGNNCC 2 cut(s) 397, 535
NmuCI GTSAC 4 cut(s) 50, 83, 563, 782
NsbI TGCGCA 1 cut(s) 1330
NspI RCATGY 2 cut(s) 1005, 1369
NspV TTCGAA 1 cut(s) 526
PaeI GCATGC 1 cut(s) 1005
PcsI WCGNNNNNNNCGW 1 cut(s) 315
PctI GAATGC 1 cut(s) 1411
PdmI GAANNNNTTC 2 cut(s) 481, 532
PfeI GAWTC 5 cut(s) 176, 332, 338, 962, 1145
PflMI CCANNNNNTGG 1 cut(s) 473
PkrI GCNGC 2 cut(s) 268, 271
PleI GAGTC 1 cut(s) 80
PpsI GAGTC 1 cut(s) 80
Ppu21I YACGTR 1 cut(s) 103
PshBI ATTAAT 1 cut(s) 1302
PsiI TTATAA 1 cut(s) 702
Psp6I CCWGG 4 cut(s) 46, 391, 446, 472
PspFI CCCAGC 1 cut(s) 64
PspGI CCWGG 4 cut(s) 46, 391, 446, 472
PspN4I GGNNCC 2 cut(s) 397, 535
PspPI GGNCC 1 cut(s) 229
PsrI GAACNNNNNNTAC 2 cut(s) 226, 258
PstNI CAGNNNCTG 1 cut(s) 473
RsaI GTAC 4 cut(s) 116, 748, 933, 1364
RsaNI GTAC 4 cut(s) 115, 747, 932, 1363
RseI CAYNNNNRTG 2 cut(s) 293, 1220
SaqAI TTAA 6 cut(s) 632, 968, 975, 1161, 1233, 1302
SatI GCNGC 2 cut(s) 267, 270
Sau3AI GATC 4 cut(s) 367, 649, 1020, 1075
Sau96I GGNCC 1 cut(s) 229
SchI GAGTC 1 cut(s) 80
ScrFI CCNGG 4 cut(s) 48, 393, 448, 474
SduI GDGCHC 1 cut(s) 736
SfaNI GCATC 1 cut(s) 800
SfuI TTCGAA 1 cut(s) 526
SinI GGWCC 1 cut(s) 229
SmiMI CAYNNNNRTG 2 cut(s) 293, 1220
SmlI CTYRAG 3 cut(s) 488, 692, 1006
SmoI CTYRAG 3 cut(s) 488, 692, 1006
SnaBI TACGTA 1 cut(s) 103
SphI GCATGC 1 cut(s) 1005
SsiI CCGC 5 cut(s) 127, 266, 272, 345, 537
SspI AATATT 1 cut(s) 480
StyD4I CCNGG 4 cut(s) 46, 391, 446, 472
StyI CCWWGG 2 cut(s) 423, 1446
TaaI ACNGT 2 cut(s) 139, 1049
TaiI ACGT 1 cut(s) 105
TaqI TCGA 7 cut(s) 91, 133, 526, 648, 808, 1019, 1074
TaqII GACCGA 1 cut(s) 246
TatI WGTACW 2 cut(s) 746, 1362
TauI GCSGC 1 cut(s) 269
TfiI GAWTC 5 cut(s) 176, 332, 338, 962, 1145
Tru1I TTAA 6 cut(s) 632, 968, 975, 1161, 1233, 1302
Tru9I TTAA 6 cut(s) 632, 968, 975, 1161, 1233, 1302
TscAI CASTG 8 cut(s) 300, 331, 382, 715, 902, 907, 1030, 1227
TseFI GTSAC 4 cut(s) 50, 83, 563, 782
TseI GCWGC 1 cut(s) 269
Tsp45I GTSAC 4 cut(s) 50, 83, 563, 782
TspDTI ATGAA 7 cut(s) 168, 330, 345, 636, 648, 1118, 1385
TspRI CASTG 8 cut(s) 300, 331, 382, 715, 902, 907, 1030, 1227
Van91I CCANNNNNTGG 1 cut(s) 473
VpaK11BI GGWCC 1 cut(s) 229
VspI ATTAAT 1 cut(s) 1302
XapI RAATTY 7 cut(s) 595, 601, 793, 851, 920, 955, 1125
XceI RCATGY 2 cut(s) 1005, 1369
XcmI CCANNNNNNNNNTGG 1 cut(s) 20
XmiI GTMKAC 1 cut(s) 688
XmnI GAANNNNTTC 2 cut(s) 481, 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.