MD14G1034800.v1.1

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0000916
Physical Location & Seq
Reverse (-)
3109455 .. 3111857
2403 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1034800.v1.1.491

Sequence Viewer

Length: 2403 bp
ATGCAGCAATCTAACTCTTTGTTGCAATATTATCAAGTGATGGATACGAACTCTGGTGTATCATGTATATTGAGATTTTTGTTATGCCTTACCTTCACTGCTTGTATGTCCATGGCGTCTGTCACCATCTCTCCAGGCCAATCTCTCTCCGGCAGCCAAACAATCACCTCTCCAGGTGGAAAATTTGAGCTCGGTTTCTTCACAACAGGTAATTCTCAAAACTATTACATAGGCATTTGGTACAAAAATCTGGCCAATCGAACAGTAGTTTGGGTTGCAAACAGACAACAACCTGTTTCTGAACCGAAACTTTCAGTATTAGAGCTTGTAGAAAATGGAAACTTGACCTTAACTAGCCCCTCCAGAGTTGCAATATGGTCGACGAATTCAAGATCAATGGCCTCTAATTCTGCTTTAGCAAAGCTTCTTGACAATGGGAACTTTGTCATAACAGATGCATTTAATTCATCTGTTGTTATATGGCAAAGTTTCGATTACCTGACAAATACTTGGCTGCCGGGTGCTGAGCTTGGATACAATAATCGTACCAAGAGAAAACAAGTTCTTACACCCTGGAGAAACTCGAAAAACCCTGCACTTGGTCCTTTTTCTTTTGAGTTAGATGAAATACCTTTGCCTTATAGTATATCTTCCTATCCTGATACCTTTACTAGAGGCACAATAGAAATCAATGGTCAACTCAAGTTTTATGCCTGGGACCAAGGCTTTAGGGGATGGACTTTGCTTTCTGTTGAACCATCGGACCAGTGTGCGGTCCAGGGGTCGTGTGGTGCTTTTAGTATTTGCCACCAGCAGAGCTCTCCTCGTTGTGGTTGCCTCAAAGGATTTGAACCCAAAGTCAGGGAAAATTGGAAACTAGAGGACTACTCAGATGGTTGTGTGAGGAAAACTCCTTTTCACTGCAGCCCAGGGGAAAGTTACTCATTTTTGACGTTAACTGATGTTGAAAAACGATACAATAAAAACTTTAAGGAAAATTTCTATGTGAGCATTGATCAATGCAGATTAGAATGCTTAATAAACTGCTCATGCACTGGCTTTACTTATAATTCTTTCGATAGTGGTCGGTGTTACATGTGGATAGGGGATCTATATGATATAGTGGCAACCTCCGAATCTCATTCCATAAGTTATCCTCCATTCGTCACAGGATTGAATATCTGGAATGTGGATTCTACAAGTGAGATGAAGAGAAAGACTACTTGGATTGTGATTGGAGTAATTGCAGGGTTCTTTTCCATCTTAAGTGCTGCCACTGTCGTGGTATTTTTCAGAAGGAGATGGTCAGCAGGAGCATTTGTGACTACGGACGATTTTTTGGTGCTTTACAAGCATAGAGATTTAAGAAGAGCAACAAACAACTTCTCCGAAAAACTCTGGGAAGGAGCCTTTGGTTCTGTATTCAAGGGGATGTTGCCTAACTCAACAGCCATAGCAGTGAAAGAGCTGAAAAGTATGAACCAGGGAGAGAAACAATTTCGTGCTGAAGTGGGAACTATCGGCGCAATCCACCACATTAATCTTATTCGCCTCCGGGGATTCTGCACGAACGCTTCGAAGAGGTTTTTAGTTTATGATTACATGCCAAATGGTTCTCTGCATTCTCTTCTACTTGGAAACAACCCAATAGTGTTGGATTGGAAGGCTAGATATCACATTGCAGTAGGGATTGCGAGAGGATTGGCTTACCTCCATGAAGAATGCAGAGACTGCATAATACACTGCGATGTTAAGCCAGAGAACATCTTGTTGGATTCAGAATACAGTCCCAAATTGGCGGATTTTGGTCTTGCAAAACTCTTGGGGAGACACCACAGCCGGGTTTTAACAACCATGAGGGGAACTGTGGGCTATCTCGCACCAGAATGGTTTTCGGGTGAAGCCATCACACCAAAAGCCGATGTCTTTAGCTATGGCATGCTGCTGATTGAGGTCATATCAGGGAGGAGAAACATGGAAGGGTTAGATAACGGGGTTGAAAACTTCCGTCCTTTGTGGCTCGCTAACGCAGTTAATAAAGTGGAAGATCTTTTCGCCGTGTTAGATTGCAGGTTGGAAGGCCAAGCTGATGGAGATGAACTAAGTAGAGTGTGCAAAGTGGCTTGTTGGTGCATTCAAGAAGATGAGAAGGATAGGCCAACAATGCGGCAGGTGGTTCAAATTCTTGAGGGCGTTTCCGACATCGGTACTCCGCCAGTCCCGCAGTTCCGCCAGCGCCTTATCAAATTTCCGGCAGAAGGCATTAACTACCAGGAGACTACGTCCGGTTCGGGTGCATGGTTATGCTCCGATGTCCAAAGTGCATTTCAAGCCGAAGTCAGCAACTGTGCAACGAAAAGTCATGAGGATCAGATGAAGGGCGTGAGGGTTCTAGTATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_2 Pkinase S_locus_glycop

Protein Analysis

801

Amino Acids

89.69

Weight (kDa)

7.13

Isoelectric Point (pI)

43.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 88 - 193 5.2e-31 D-mannose binding lectin
S_locus_glycop PF00954 208 - 286 3.2e-16 S-locus glycoprotein domain
PAN_2 PF08276 316 - 374 3.2e-09 PAN-like domain
PK_Tyr_Ser-Thr PF07714 462 - 729 9.8e-41 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 463 - 727 2.6e-47 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1068
AarI CACCTGC 1 cut(s) 2161
Acc36I ACCTGC 2 cut(s) 2061, 2161
AccI GTMKAC 1 cut(s) 380
AciI CCGC 6 cut(s) 773, 1799, 2167, 2213, 2222, 2230
AclWI GGATC 2 cut(s) 1116, 2376
AcoI YGGCCR 1 cut(s) 252
AcsI RAATTY 5 cut(s) 182, 385, 997, 2181, 2246
AcuI CTGAAG 1 cut(s) 1527
AcyI GRCGYC 1 cut(s) 116
AfaI GTAC 3 cut(s) 242, 547, 2209
AfiI CCNNNNNNNGG 6 cut(s) 599, 772, 830, 861, 1840, 2258
AflII CTTAAG 1 cut(s) 1264
AflIII ACRYGT 1 cut(s) 1095
AjnI CCWGG 8 cut(s) 133, 172, 572, 713, 777, 928, 1482, 2271
AleI CACNNNNGTG 1 cut(s) 1280
AluBI AGCT 8 cut(s) 190, 325, 424, 529, 819, 1468, 1932, 2087
AluI AGCT 8 cut(s) 190, 325, 424, 529, 819, 1468, 1932, 2087
Alw21I GWGCWC 2 cut(s) 192, 821
Alw26I GTCTC 3 cut(s) 1722, 1822, 2270
AlwI GGATC 2 cut(s) 1116, 2376
AlwNI CAGNNNCTG 2 cut(s) 1731, 2346
AoxI GGCC 5 cut(s) 136, 252, 399, 2080, 2156
ApeKI GCWGC 6 cut(s) 4, 153, 514, 924, 1271, 1942
ApoI RAATTY 5 cut(s) 182, 385, 997, 2181, 2246
AseI ATTAAT 1 cut(s) 1539
AspLEI GCGC 2 cut(s) 1526, 2238
AspS9I GGNCC 4 cut(s) 602, 718, 763, 775
AsuC2I CCSGG 3 cut(s) 519, 1556, 1841
AsuHPI GGTGA 3 cut(s) 115, 157, 1910
AsuII TTCGAA 1 cut(s) 1577
AvaII GGWCC 4 cut(s) 602, 718, 763, 775
BalI TGGCCA 1 cut(s) 254
BanII GRGCYC 2 cut(s) 192, 821
Bbv12I GWGCWC 2 cut(s) 192, 821
BbvI GCAGC 6 cut(s) 16, 165, 501, 936, 1258, 1929
BccI CCATC 9 cut(s) 34, 134, 729, 766, 887, 1268, 1296, 1913, 2084
BceAI ACGGC 1 cut(s) 2042
BcgI CGANNNNNNTGC 2 cut(s) 360, 394
BciT130I CCWGG 8 cut(s) 135, 174, 574, 715, 779, 930, 1484, 2273
BciVI GTATCC 2 cut(s) 37, 527
BclI TGATCA 1 cut(s) 1015
BcnI CCSGG 3 cut(s) 519, 1556, 1841
BcoDI GTCTC 3 cut(s) 1722, 1822, 2270
BfaI CTAG 5 cut(s) 354, 672, 878, 1668, 2393
BfmI CTRYAG 1 cut(s) 922
BfoI RGCGCY 1 cut(s) 2239
BfrI CTTAAG 1 cut(s) 1264
BfuAI ACCTGC 2 cut(s) 2061, 2161
BfuI GTATCC 2 cut(s) 37, 527
BglII AGATCT 1 cut(s) 2047
BisI GCNGC 7 cut(s) 5, 154, 515, 925, 1272, 1943, 2168
BlpI GCTNAGC 1 cut(s) 525
BlsI GCNGC 7 cut(s) 6, 155, 516, 926, 1273, 1944, 2169
Bme18I GGWCC 4 cut(s) 602, 718, 763, 775
BmgT120I GGNCC 4 cut(s) 602, 718, 763, 775
BmiI GGNNCC 2 cut(s) 719, 1408
BmsI GCATC 1 cut(s) 445
BplI GAGNNNNNCTC 6 cut(s) 808, 840, 872, 904, 895, 927
BpmI CTGGAG 4 cut(s) 117, 156, 346, 595
Bpu1102I GCTNAGC 1 cut(s) 525
Bpu14I TTCGAA 1 cut(s) 1577
BpuEI CTTGAG 2 cut(s) 686, 2207
BpuMI CCSGG 3 cut(s) 519, 1556, 1841
BsaHI GRCGYC 1 cut(s) 116
BsaJI CCNNGG 9 cut(s) 111, 572, 714, 721, 778, 928, 929, 1483, 1555
BsaWI WCCGGW 1 cut(s) 2285
BsaXI ACNNNNNCTCC 8 cut(s) 115, 145, 1305, 1335, 1370, 1400, 2267, 2297
Bsc4I CCNNNNNNNGG 6 cut(s) 599, 772, 830, 861, 1840, 2258
Bse1I ACTGG 3 cut(s) 766, 1060, 2216
Bse3DI GCAATG 1 cut(s) 1677
BseBI CCWGG 8 cut(s) 135, 174, 574, 715, 779, 930, 1484, 2273
BseDI CCNNGG 9 cut(s) 111, 572, 714, 721, 778, 928, 929, 1483, 1555
BseGI GGATG 2 cut(s) 740, 1437
BseLI CCNNNNNNNGG 6 cut(s) 599, 772, 830, 861, 1840, 2258
BseMI GCAATG 1 cut(s) 1677
BseMII CTCAG 2 cut(s) 516, 903
BseNI ACTGG 3 cut(s) 766, 1060, 2216
BseRI GAGGAG 2 cut(s) 813, 1981
BseXI GCAGC 6 cut(s) 16, 165, 501, 936, 1258, 1929
BsgI GTGCAG 2 cut(s) 579, 1549
BshFI GGCC 5 cut(s) 138, 254, 401, 2082, 2158
BsiHKAI GWGCWC 2 cut(s) 192, 821
BsiSI CCGG 6 cut(s) 150, 518, 1555, 1840, 2252, 2286
BslFI GGGAC 3 cut(s) 731, 1773, 2204
BslI CCNNNNNNNGG 6 cut(s) 599, 772, 830, 861, 1840, 2258
BsmAI GTCTC 3 cut(s) 1722, 1822, 2270
BsmFI GGGAC 3 cut(s) 731, 1773, 2204
BsmI GAATGC 4 cut(s) 1037, 1621, 1727, 2133
BsnI GGCC 5 cut(s) 138, 254, 401, 2082, 2158
Bsp119I TTCGAA 1 cut(s) 1577
Bsp1286I GDGCHC 2 cut(s) 192, 821
Bsp143I GATC 5 cut(s) 392, 1015, 1108, 2047, 2368
Bsp1720I GCTNAGC 1 cut(s) 525
Bsp19I CCATGG 1 cut(s) 111
BspACI CCGC 6 cut(s) 773, 1799, 2167, 2213, 2222, 2230
BspANI GGCC 5 cut(s) 138, 254, 401, 2082, 2158
BspCNI CTCAG 2 cut(s) 517, 902
BspHI TCATGA 1 cut(s) 2362
BspLI GGNNCC 2 cut(s) 719, 1408
BspMAI CTGCAG 1 cut(s) 926
BspMI ACCTGC 2 cut(s) 2061, 2161
BspPI GGATC 2 cut(s) 1116, 2376
BspQI GCTCTTC 1 cut(s) 1363
BspT104I TTCGAA 1 cut(s) 1577
BspTI CTTAAG 1 cut(s) 1264
BsrDI GCAATG 1 cut(s) 1677
BsrI ACTGG 3 cut(s) 766, 1060, 2216
BssECI CCNNGG 9 cut(s) 111, 572, 714, 721, 778, 928, 929, 1483, 1555
BssMI GATC 5 cut(s) 392, 1015, 1108, 2047, 2368
BssNI GRCGYC 1 cut(s) 116
BssT1I CCWWGG 2 cut(s) 111, 721
Bst2UI CCWGG 8 cut(s) 135, 174, 574, 715, 779, 930, 1484, 2273
Bst4CI ACNGT 5 cut(s) 265, 1279, 1787, 1867, 2348
Bst6I CTCTTC 4 cut(s) 1205, 1363, 1574, 1632
BstACI GRCGYC 1 cut(s) 116
BstAFI CTTAAG 1 cut(s) 1264
BstAPI GCANNNNNTGC 1 cut(s) 1731
BstBI TTCGAA 1 cut(s) 1577
BstC8I GCNNGC 3 cut(s) 1940, 2022, 2234
BstDEI CTNAG 3 cut(s) 525, 889, 2102
BstDSI CCRYGG 1 cut(s) 111
BstF5I GGATG 2 cut(s) 740, 1437
BstH2I RGCGCY 1 cut(s) 2239
BstHHI GCGC 2 cut(s) 1526, 2238
BstKTI GATC 5 cut(s) 395, 1018, 1111, 2050, 2371
BstMAI GTCTC 3 cut(s) 1722, 1822, 2270
BstMBI GATC 5 cut(s) 392, 1015, 1108, 2047, 2368
BstMWI GCNNNNNNNGC 5 cut(s) 1351, 1731, 2164, 2221, 2330
BstNI CCWGG 8 cut(s) 135, 174, 574, 715, 779, 930, 1484, 2273
BstNSI RCATGY 3 cut(s) 1099, 1606, 1942
BstSFI CTRYAG 1 cut(s) 922
BstV1I GCAGC 6 cut(s) 16, 165, 501, 936, 1258, 1929
BstX2I RGATCY 2 cut(s) 1108, 2047
BstXI CCANNNNNNTGG 2 cut(s) 1282, 2090
BstYI RGATCY 2 cut(s) 1108, 2047
BsuI GTATCC 2 cut(s) 37, 527
BsuRI GGCC 5 cut(s) 138, 254, 401, 2082, 2158
BtgI CCRYGG 1 cut(s) 111
BtgZI GCGATG 1 cut(s) 1761
BtsCI GGATG 2 cut(s) 740, 1437
BtsI GCAGTG 4 cut(s) 96, 919, 1464, 1741
BtsIMutI CAGTG 7 cut(s) 96, 773, 919, 1053, 1275, 1464, 1741
BveI ACCTGC 2 cut(s) 2061, 2161
Cac8I GCNNGC 3 cut(s) 1940, 2022, 2234
CaiI CAGNNNCTG 2 cut(s) 1731, 2346
CciI TCATGA 1 cut(s) 2362
CfoI GCGC 2 cut(s) 1526, 2238
Cfr13I GGNCC 4 cut(s) 602, 718, 763, 775
CseI GACGC 1 cut(s) 105
Csp6I GTAC 3 cut(s) 241, 546, 2208
CviQI GTAC 3 cut(s) 241, 546, 2208
DdeI CTNAG 3 cut(s) 525, 889, 2102
DpnI GATC 5 cut(s) 394, 1017, 1110, 2049, 2370
DpnII GATC 5 cut(s) 392, 1015, 1108, 2047, 2368
EaeI YGGCCR 1 cut(s) 252
Eam1104I CTCTTC 4 cut(s) 1205, 1363, 1574, 1632
EarI CTCTTC 4 cut(s) 1205, 1363, 1574, 1632
EciI GGCGGA 3 cut(s) 1814, 2202, 2219
Ecl136II GAGCTC 2 cut(s) 190, 819
Eco130I CCWWGG 2 cut(s) 111, 721
Eco24I GRGCYC 2 cut(s) 192, 821
Eco32I GATATC 1 cut(s) 1673
Eco47I GGWCC 4 cut(s) 602, 718, 763, 775
Eco53kI GAGCTC 2 cut(s) 190, 819
Eco57I CTGAAG 1 cut(s) 1527
EcoICRI GAGCTC 2 cut(s) 190, 819
EcoRI GAATTC 1 cut(s) 385
EcoRII CCWGG 8 cut(s) 133, 172, 572, 713, 777, 928, 1482, 2271
EcoRV GATATC 1 cut(s) 1673
EcoT14I CCWWGG 2 cut(s) 111, 721
EcoT22I ATGCAT 1 cut(s) 460
EcoT38I GRGCYC 2 cut(s) 192, 821
ErhI CCWWGG 2 cut(s) 111, 721
FaqI GGGAC 3 cut(s) 731, 1773, 2204
FauI CCCGC 1 cut(s) 2229
FbaI TGATCA 1 cut(s) 1015
FblI GTMKAC 1 cut(s) 380
Fnu4HI GCNGC 7 cut(s) 5, 154, 515, 925, 1272, 1943, 2168
FokI GGATG 2 cut(s) 747, 1444
FriOI GRGCYC 2 cut(s) 192, 821
Fsp4HI GCNGC 7 cut(s) 5, 154, 515, 925, 1272, 1943, 2168
FspBI CTAG 5 cut(s) 354, 672, 878, 1668, 2393
GlaI GCGC 2 cut(s) 1525, 2237
GluI GCNGC 7 cut(s) 5, 154, 515, 925, 1272, 1943, 2168
GsuI CTGGAG 4 cut(s) 117, 156, 346, 595
HaeII RGCGCY 1 cut(s) 2239
HaeIII GGCC 5 cut(s) 138, 254, 401, 2082, 2158
HapII CCGG 6 cut(s) 150, 518, 1555, 1840, 2252, 2286
HgaI GACGC 1 cut(s) 105
HhaI GCGC 2 cut(s) 1526, 2238
Hin1I GRCGYC 1 cut(s) 116
Hin6I GCGC 2 cut(s) 1524, 2236
HinP1I GCGC 2 cut(s) 1524, 2236
HincII GTYRAC 3 cut(s) 381, 698, 957
HindII GTYRAC 3 cut(s) 381, 698, 957
HindIII AAGCTT 1 cut(s) 422
HinfI GANTC 4 cut(s) 1136, 1193, 1560, 1775
HpaI GTTAAC 1 cut(s) 957
HpaII CCGG 6 cut(s) 150, 518, 1555, 1840, 2252, 2286
HphI GGTGA 3 cut(s) 115, 157, 1910
Hpy166II GTNNAC 3 cut(s) 381, 698, 957
Hpy188III TCNNGA 8 cut(s) 363, 390, 428, 659, 1183, 2138, 2186, 2363
Hpy8I GTNNAC 3 cut(s) 381, 698, 957
Hpy99I CGWCG 1 cut(s) 385
HpyAV CCTTC 9 cut(s) 103, 1290, 1397, 1657, 1973, 2072, 2143, 2252, 2371
HpyCH4III ACNGT 5 cut(s) 265, 1279, 1787, 1867, 2348
HpyCH4IV ACGT 2 cut(s) 953, 2282
HpyF10VI GCNNNNNNNGC 5 cut(s) 1351, 1731, 2164, 2221, 2330
HpyF3I CTNAG 3 cut(s) 525, 889, 2102
HpySE526I ACGT 2 cut(s) 953, 2282
Hsp92I GRCGYC 1 cut(s) 116
HspAI GCGC 2 cut(s) 1524, 2236
Ksp22I TGATCA 1 cut(s) 1015
KspAI GTTAAC 1 cut(s) 957
Kzo9I GATC 5 cut(s) 392, 1015, 1108, 2047, 2368
LguI GCTCTTC 1 cut(s) 1363
LmnI GCTCC 3 cut(s) 1313, 1406, 2312
Lsp1109I GCAGC 6 cut(s) 16, 165, 501, 936, 1258, 1929
LweI GCATC 1 cut(s) 445
MaeI CTAG 5 cut(s) 354, 672, 878, 1668, 2393
MaeII ACGT 2 cut(s) 953, 2282
MaeIII GTNAC 5 cut(s) 121, 938, 1091, 1165, 1321
MalI GATC 5 cut(s) 394, 1017, 1110, 2049, 2370
MboI GATC 5 cut(s) 392, 1015, 1108, 2047, 2368
MboII GAAGA 9 cut(s) 190, 642, 1222, 1380, 1591, 1619, 1730, 2057, 2153
MflI RGATCY 2 cut(s) 1108, 2047
MhlI GDGCHC 2 cut(s) 192, 821
MlsI TGGCCA 1 cut(s) 254
MluNI TGGCCA 1 cut(s) 254
MmeI TCCRAC 4 cut(s) 1635, 1752, 2055, 2223
Mox20I TGGCCA 1 cut(s) 254
Mph1103I ATGCAT 1 cut(s) 460
MscI TGGCCA 1 cut(s) 254
MslI CAYNNNNRTG 5 cut(s) 1280, 1457, 1746, 1885, 2302
Msp20I TGGCCA 1 cut(s) 254
MspCI CTTAAG 1 cut(s) 1264
MspI CCGG 6 cut(s) 150, 518, 1555, 1840, 2252, 2286
Mva1269I GAATGC 4 cut(s) 1037, 1621, 1727, 2133
MvaI CCWGG 8 cut(s) 135, 174, 574, 715, 779, 930, 1484, 2273
MwoI GCNNNNNNNGC 5 cut(s) 1351, 1731, 2164, 2221, 2330
NciI CCSGG 3 cut(s) 519, 1556, 1841
NcoI CCATGG 1 cut(s) 111
NdeII GATC 5 cut(s) 392, 1015, 1108, 2047, 2368
NlaIV GGNNCC 2 cut(s) 719, 1408
NmuCI GTSAC 3 cut(s) 121, 1165, 1321
NsiI ATGCAT 1 cut(s) 460
NspI RCATGY 3 cut(s) 1099, 1606, 1942
NspV TTCGAA 1 cut(s) 1577
OliI CACNNNNGTG 1 cut(s) 1280
PaeI GCATGC 1 cut(s) 1942
PagI TCATGA 1 cut(s) 2362
PaqCI CACCTGC 1 cut(s) 2161
PasI CCCWGGG 1 cut(s) 929
PciI ACATGT 1 cut(s) 1095
PciSI GCTCTTC 1 cut(s) 1363
PcsI WCGNNNNNNNCGW 1 cut(s) 1574
PctI GAATGC 4 cut(s) 1037, 1621, 1727, 2133
PfeI GAWTC 4 cut(s) 1136, 1193, 1560, 1775
PflFI GACNNNGTC 1 cut(s) 2281
PkrI GCNGC 7 cut(s) 6, 155, 516, 926, 1273, 1944, 2169
PscI ACATGT 1 cut(s) 1095
PshBI ATTAAT 1 cut(s) 1539
PsiI TTATAA 1 cut(s) 1068
Psp124BI GAGCTC 2 cut(s) 192, 821
Psp6I CCWGG 8 cut(s) 133, 172, 572, 713, 777, 928, 1482, 2271
PspGI CCWGG 8 cut(s) 133, 172, 572, 713, 777, 928, 1482, 2271
PspN4I GGNNCC 2 cut(s) 719, 1408
PspPI GGNCC 4 cut(s) 602, 718, 763, 775
PstI CTGCAG 1 cut(s) 926
PstNI CAGNNNCTG 2 cut(s) 1731, 2346
PsuI RGATCY 2 cut(s) 1108, 2047
PsyI GACNNNGTC 1 cut(s) 2281
RsaI GTAC 3 cut(s) 242, 547, 2209
RsaNI GTAC 3 cut(s) 241, 546, 2208
RseI CAYNNNNRTG 5 cut(s) 1280, 1457, 1746, 1885, 2302
SacI GAGCTC 2 cut(s) 192, 821
SalI GTCGAC 1 cut(s) 379
SapI GCTCTTC 1 cut(s) 1363
SatI GCNGC 7 cut(s) 5, 154, 515, 925, 1272, 1943, 2168
Sau3AI GATC 5 cut(s) 392, 1015, 1108, 2047, 2368
Sau96I GGNCC 4 cut(s) 602, 718, 763, 775
SduI GDGCHC 2 cut(s) 192, 821
SfaNI GCATC 1 cut(s) 445
SfcI CTRYAG 1 cut(s) 922
SfuI TTCGAA 1 cut(s) 1577
SinI GGWCC 4 cut(s) 602, 718, 763, 775
SmiMI CAYNNNNRTG 5 cut(s) 1280, 1457, 1746, 1885, 2302
SmlI CTYRAG 3 cut(s) 701, 1264, 2186
SmoI CTYRAG 3 cut(s) 701, 1264, 2186
SphI GCATGC 1 cut(s) 1942
SsiI CCGC 6 cut(s) 773, 1799, 2167, 2213, 2222, 2230
SspI AATATT 1 cut(s) 29
SspMI CTAG 5 cut(s) 354, 672, 878, 1668, 2393
SstI GAGCTC 2 cut(s) 192, 821
StyI CCWWGG 2 cut(s) 111, 721
TaaI ACNGT 5 cut(s) 265, 1279, 1787, 1867, 2348
TaiI ACGT 2 cut(s) 956, 2285
TaqI TCGA 6 cut(s) 259, 380, 492, 584, 1077, 1577
TauI GCSGC 1 cut(s) 2170
TfiI GAWTC 4 cut(s) 1136, 1193, 1560, 1775
TscAI CASTG 7 cut(s) 103, 773, 926, 1060, 1282, 1464, 1748
TseFI GTSAC 3 cut(s) 121, 1165, 1321
TseI GCWGC 6 cut(s) 4, 153, 514, 924, 1271, 1942
Tsp45I GTSAC 3 cut(s) 121, 1165, 1321
TspDTI ATGAA 7 cut(s) 456, 639, 1223, 1493, 1731, 2112, 2390
TspGWI ACGGA 2 cut(s) 1343, 1997
TspRI CASTG 7 cut(s) 103, 773, 926, 1060, 1282, 1464, 1748
Tth111I GACNNNGTC 1 cut(s) 2281
Vha464I CTTAAG 1 cut(s) 1264
VpaK11BI GGWCC 4 cut(s) 602, 718, 763, 775
VspI ATTAAT 1 cut(s) 1539
XapI RAATTY 5 cut(s) 182, 385, 997, 2181, 2246
XceI RCATGY 3 cut(s) 1099, 1606, 1942
XcmI CCANNNNNNNNNTGG 1 cut(s) 785
XmiI GTMKAC 1 cut(s) 380
XspI CTAG 5 cut(s) 354, 672, 878, 1668, 2393
Zsp2I ATGCAT 1 cut(s) 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.