RLG00000018934

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000916
Physical Location & Seq
Reverse (-)
43099888 .. 43104093
4206 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018934

Sequence Viewer

Length: 2391 bp
ATGAAAACTAGCATCAGAAACCACTTGATTTCCCAGTCTGTGCTGCTCTTGTTATCTTACACTACTTGTACTCTCAAGGCTATTGAGATTCTGTTACCAGGCCAATCTCTCAATGGGGACCAGAAGATCACGTCTCCTGGTGGCATCTTTGAACTTGGTTTTTTCACACCAGGTAACTCTCAAAACTATTACATAGGCATCTGGTACAAAAATCTACCCACACAAACAGTTGTTTGGGTGGCAAACAGAAACCAACCAATTATCGATCCAACTTCCTCATCCTTTGTGCTCCTTGAAAATGGAAATTTGACCTTAAACAACCCCTCAACCATTGCAATATGGTCAACAAATTCAATGTCACAGGTTAGAAATTCTACTATAGCCATGCATCCTGACGATGGCAACTTTGTCATAAGAGAAGCATCTGCAGTACTATGGCAAAGTTTTGATCATCCAACCAATACTTGGCTGCCAGGTGCAAGGCTTGGATATAACAAGCTTACCAAGCAAATCCTAAGTCTTACTCCCTGGAGAAATCCACAACACCCAGCACCTGGTCCCTTTTCTTTTGAGATAGAACAAACTAGTCTTCGGCTATTATGGAATAGTACTTCTGAAACTTACTGGACCAGCGGTAATTGGACTGGGAAAACTTTCAGCCATCTTCAAGACATAGAAGAGAAGCATGTTCCCTCAATTTTCTCATTTGTTTTAAATGAGACTGGAAGCTACCTTAGTTATGCAGTTCGTGCTTCTGCCTATCCTGATCCCTTTATTAGAAATCATACCTTTATTAGGTACATGATAGAAACCACTGGTCAAATGAAGTTCTTTGTCATGGAGAACGGCTCCCGGCAATGGAATTTGCTATGGATGGATCCAATCGAGCGATGTGATGTTTATGCTTCCTGTGGTGCTTTTAGTATATGCAACCCACCTAATCTTCCTCGGTGTGGCTGTCTAGAAGGATTCGAACCCAAATTCCCTAAAGACTGGGAATTAGAAGATCACTCAAATGGCTGTGGGAGGAAAACTTCTTTTCACTGCAGTGATGACTGGAATTCTGCATTCCTGTTGATGGAGAATGTTACTTACCTGAATAGTCCGGACTCCTTATTGGTAGTAGATAACACTGACAAATGTAGATTAACTTGCTTGAGAAATTGCTCATGCACTGCTTTTGCTTATGATAATCAGTGTCTGATTTGGGGAGGTGATCTTTTTGATGTAAAGCAACTATCATCTAAGGGAATGGTGGGAAAAGTTTGGTATCTACGAGTAGCAACATCTGAGAAGGAAATAATAGATTCTACAGATGGGAATAGAAAGAGAAAGACTACTTGGATTGTGGTTGGAGTACTTGCAGGCATCTTTACTCTCTTTAGCATTGTGCTTATACTTGTCAGACAGAGACGGTCTATTCATCAAGCATTGAAACCTTTTGAACATTCTCTAGTGCTGTTCAACTACAGAGATTTAAGAAAAGCAACAGAAAACTTCTCAGAGAAACTTGGAGAAGGAGCTTTCGGTTCTGTTTTCAAGGGGATATTGCCTGATTCAACTCCCATAGCAGTGAAACAACTAAAGAGTCTGTTGCAGGGTGAAAAGCAATTTCAGGCTGAGGTGAGCACTCTTGGTGCAATCCAACACATTAATCTTCTTCGCCTCCATGGGTTTTGTGCTGATTCTACAAGAAGACTCTTAGTTTATGACTACATGCCAAATGGCTCTCTACAATCTGTTCTAGTTCAAAACCATCCACTAATCTTAGATTGGAAAGCAAGATATCATATTGCCATAGGCATTGCAAGAGGATTGTCCTACCTCCATGAGGACTGTAGAGATTGTATAATACACTGTGACATTAAGCCAGAGAACATTTTGTTGGATGAAAAATATAGTCCAAAAGTTGCAGACTTTGGTCTGGCAAAACTCATTGGCAGACACCATAGTCGAGTTCTGACGAGTGTGAGAGGGACTCTTGGATATCTAGCACCAGAATGGTTTACAGGTGAAGCCATCACTCCAAAAGCCGATGTCTTCAGCTATGGAAAGTTGCTGTTTGAGGTTATATCTGGAAGGAGAAACAGTGAAGGGTTAGATGATGGATTTGAAAACTACAGTCCTATCCAGATTGCAAATGTATTAACTAAAGGACAGGATGTTCTTACCTTGGTTGATTACAGGTTGGGAGGTAATGCTGACAAGGATGAACTCATTAGAGCATGCAAAGTTGCGTGTTGGTGCATTCAAGATGAGGAGAAGGATAGGCCAACAATGAGGCAGGTTGTTCAAATTCTCGAAGGAGCTTTAGATGTTGTGTGTTTCTATTTCACCATAGCATCAGAGCTTGATCCCATTCAGTCATTGAGTGGGAAGGAAGTCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_2 Pkinase S_locus_glycop

Protein Analysis

797

Amino Acids

89.79

Weight (kDa)

6.46

Isoelectric Point (pI)

41.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 76 - 178 2.3e-28 D-mannose binding lectin
S_locus_glycop PF00954 209 - 328 1.1e-21 S-locus glycoprotein domain
PAN_2 PF08276 354 - 410 4.9e-11 PAN-like domain
PK_Tyr_Ser-Thr PF07714 500 - 767 3.3e-42 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 501 - 765 2.7e-47 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1950
Acc36I ACCTGC 1 cut(s) 2275
AccB7I CCANNNNNTGG 2 cut(s) 465, 554
AccIII TCCGGA 1 cut(s) 1105
AciI CCGC 1 cut(s) 633
AclWI GGATC 5 cut(s) 260, 761, 872, 885, 2348
AcsI RAATTY 7 cut(s) 304, 349, 370, 862, 980, 1060, 2295
AcuI CTGAAG 1 cut(s) 2026
AfaI GTAC 6 cut(s) 70, 206, 432, 610, 800, 1359
AfiI CCNNNNNNNGG 8 cut(s) 398, 465, 554, 795, 858, 953, 1078, 1831
AhlI ACTAGT 1 cut(s) 584
AjiI CACGTC 1 cut(s) 132
AjnI CCWGG 6 cut(s) 97, 136, 169, 472, 527, 553
AjuI GAANNNNNNNTTGG 4 cut(s) 1324, 1356, 1868, 1900
AleI CACNNNNGTG 1 cut(s) 1047
AloI GAACNNNNNNTCC 4 cut(s) 966, 998, 2148, 2180
AluBI AGCT 6 cut(s) 499, 729, 1523, 2046, 2309, 2350
AluI AGCT 6 cut(s) 499, 729, 1523, 2046, 2309, 2350
Alw21I GWGCWC 2 cut(s) 291, 1631
Alw26I GTCTC 3 cut(s) 138, 713, 1405
AlwI GGATC 5 cut(s) 260, 761, 872, 885, 2348
AlwNI CAGNNNCTG 2 cut(s) 554, 1201
Aor13HI TCCGGA 1 cut(s) 1105
AoxI GGCC 2 cut(s) 100, 2270
ApeKI GCWGC 2 cut(s) 43, 469
ApoI RAATTY 7 cut(s) 304, 349, 370, 862, 980, 1060, 2295
ArsI GACNNNNNNTTYG 4 cut(s) 341, 373, 502, 534
AseI ATTAAT 1 cut(s) 1653
Asp700I GAANNNNTTC 1 cut(s) 653
AspS9I GGNCC 3 cut(s) 118, 557, 627
AsuC2I CCSGG 1 cut(s) 853
AsuHPI GGTGA 5 cut(s) 1226, 1613, 1636, 2024, 2326
AsuII TTCGAA 1 cut(s) 972
AvaII GGWCC 3 cut(s) 118, 557, 627
BamHI GGATCC 1 cut(s) 877
BbsI GAAGAC 3 cut(s) 581, 1702, 2032
Bbv12I GWGCWC 2 cut(s) 291, 1631
BbvCI CCTCAGC 1 cut(s) 1620
BbvI GCAGC 2 cut(s) 30, 456
BccI CCATC 8 cut(s) 392, 669, 868, 1072, 1310, 1764, 2027, 2099
BceAI ACGGC 1 cut(s) 862
BciT130I CCWGG 6 cut(s) 99, 138, 171, 474, 529, 555
BclI TGATCA 1 cut(s) 448
BcnI CCSGG 1 cut(s) 853
BcoDI GTCTC 3 cut(s) 138, 713, 1405
BcuI ACTAGT 1 cut(s) 584
BfaI CTAG 6 cut(s) 9, 585, 962, 1454, 1745, 1991
BfmI CTRYAG 7 cut(s) 378, 426, 1045, 1311, 1468, 1837, 2119
BfuAI ACCTGC 1 cut(s) 2275
BisI GCNGC 2 cut(s) 44, 470
BlsI GCNGC 2 cut(s) 45, 471
BmcAI AGTACT 3 cut(s) 432, 610, 1359
Bme1390I CCNGG 7 cut(s) 99, 138, 171, 474, 529, 555, 853
Bme18I GGWCC 3 cut(s) 118, 557, 627
BmgBI CACGTC 1 cut(s) 132
BmgT120I GGNCC 3 cut(s) 118, 557, 627
BmiI GGNNCC 4 cut(s) 119, 559, 850, 879
BmrFI CCNGG 7 cut(s) 99, 138, 171, 474, 529, 555, 853
BmrI ACTGGG 3 cut(s) 28, 654, 1003
BmsI GCATC 7 cut(s) 21, 153, 207, 397, 431, 1377, 2351
BmuI ACTGGG 3 cut(s) 28, 654, 1003
BoxI GACNNNNGTC 1 cut(s) 1920
BpiI GAAGAC 3 cut(s) 581, 1702, 2032
BplI GAGNNNNNCTC 4 cut(s) 833, 865, 1963, 1995
BpmI CTGGAG 1 cut(s) 550
Bpu10I CCTNAGC 1 cut(s) 1620
Bpu14I TTCGAA 1 cut(s) 972
BpuEI CTTGAG 2 cut(s) 59, 1177
BpuMI CCSGG 1 cut(s) 853
Bsa29I ATCGAT 1 cut(s) 264
BsaJI CCNNGG 4 cut(s) 527, 947, 1669, 2172
BsaWI WCCGGW 1 cut(s) 1105
BsaXI ACNNNNNCTCC 2 cut(s) 2074, 2104
Bsc4I CCNNNNNNNGG 8 cut(s) 398, 465, 554, 795, 858, 953, 1078, 1831
Bse1I ACTGG 7 cut(s) 34, 629, 649, 727, 820, 998, 1061
Bse3DI GCAATG 3 cut(s) 330, 863, 1803
BseAI TCCGGA 1 cut(s) 1105
BseBI CCWGG 6 cut(s) 99, 138, 171, 474, 529, 555
BseCI ATCGAT 1 cut(s) 264
BseDI CCNNGG 4 cut(s) 527, 947, 1669, 2172
BseGI GGATG 8 cut(s) 278, 388, 451, 879, 1756, 1894, 2167, 2215
BseLI CCNNNNNNNGG 8 cut(s) 398, 465, 554, 795, 858, 953, 1078, 1831
BseMI GCAATG 3 cut(s) 330, 863, 1803
BseMII CTCAG 3 cut(s) 1281, 1515, 1611
BseNI ACTGG 7 cut(s) 34, 629, 649, 727, 820, 998, 1061
BseRI GAGGAG 1 cut(s) 2273
BseXI GCAGC 2 cut(s) 30, 456
BseYI CCCAGC 1 cut(s) 547
BshFI GGCC 2 cut(s) 102, 2272
BshVI ATCGAT 1 cut(s) 264
BsiHKAI GWGCWC 2 cut(s) 291, 1631
BsiSI CCGG 2 cut(s) 853, 1106
BslFI GGGAC 3 cut(s) 131, 543, 1990
BslI CCNNNNNNNGG 8 cut(s) 398, 465, 554, 795, 858, 953, 1078, 1831
BsmAI GTCTC 3 cut(s) 138, 713, 1405
BsmBI CGTCTC 2 cut(s) 138, 1405
BsmFI GGGAC 3 cut(s) 131, 543, 1990
BsmI GAATGC 2 cut(s) 1067, 2247
BsnI GGCC 2 cut(s) 102, 2272
Bsp119I TTCGAA 1 cut(s) 972
Bsp1286I GDGCHC 2 cut(s) 291, 1631
Bsp13I TCCGGA 1 cut(s) 1105
Bsp143I GATC 8 cut(s) 126, 265, 448, 766, 877, 1006, 1216, 2353
Bsp19I CCATGG 1 cut(s) 1669
BspACI CCGC 1 cut(s) 633
BspANI GGCC 2 cut(s) 102, 2272
BspCNI CTCAG 3 cut(s) 1282, 1514, 1612
BspDI ATCGAT 1 cut(s) 264
BspEI TCCGGA 1 cut(s) 1105
BspLI GGNNCC 4 cut(s) 119, 559, 850, 879
BspMAI CTGCAG 2 cut(s) 430, 1049
BspMI ACCTGC 1 cut(s) 2275
BspPI GGATC 5 cut(s) 260, 761, 872, 885, 2348
BspT104I TTCGAA 1 cut(s) 972
BsrDI GCAATG 3 cut(s) 330, 863, 1803
BsrI ACTGG 7 cut(s) 34, 629, 649, 727, 820, 998, 1061
BssECI CCNNGG 4 cut(s) 527, 947, 1669, 2172
BssMI GATC 8 cut(s) 126, 265, 448, 766, 877, 1006, 1216, 2353
BssT1I CCWWGG 2 cut(s) 1669, 2172
Bst2UI CCWGG 6 cut(s) 99, 138, 171, 474, 529, 555
Bst4CI ACNGT 6 cut(s) 229, 1416, 1838, 1859, 2090, 2123
Bst6I CTCTTC 1 cut(s) 672
BstAPI GCANNNNNTGC 1 cut(s) 749
BstBI TTCGAA 1 cut(s) 972
BstC8I GCNNGC 2 cut(s) 1366, 2227
BstDEI CTNAG 8 cut(s) 515, 734, 1245, 1290, 1501, 1620, 1702, 1768
BstDSI CCRYGG 1 cut(s) 1669
BstENI CCTNNNNNAGG 2 cut(s) 793, 1829
BstF5I GGATG 8 cut(s) 278, 388, 451, 879, 1756, 1894, 2167, 2215
BstKTI GATC 8 cut(s) 129, 268, 451, 769, 880, 1009, 1219, 2356
BstMAI GTCTC 3 cut(s) 138, 713, 1405
BstMBI GATC 8 cut(s) 126, 265, 448, 766, 877, 1006, 1216, 2353
BstMWI GCNNNNNNNGC 2 cut(s) 505, 749
BstNI CCWGG 6 cut(s) 99, 138, 171, 474, 529, 555
BstNSI RCATGY 3 cut(s) 689, 1720, 2229
BstPAI GACNNNNGTC 1 cut(s) 1920
BstSCI CCNGG 7 cut(s) 97, 136, 169, 472, 527, 553, 851
BstSFI CTRYAG 7 cut(s) 378, 426, 1045, 1311, 1468, 1837, 2119
BstV1I GCAGC 2 cut(s) 30, 456
BstV2I GAAGAC 3 cut(s) 581, 1702, 2032
BstX2I RGATCY 1 cut(s) 877
BstYI RGATCY 1 cut(s) 877
Bsu15I ATCGAT 1 cut(s) 264
BsuRI GGCC 2 cut(s) 102, 2272
BsuTUI ATCGAT 1 cut(s) 264
BtgI CCRYGG 1 cut(s) 1669
BtgZI GCGATG 1 cut(s) 904
BtrI CACGTC 1 cut(s) 132
BtsCI GGATG 8 cut(s) 278, 388, 451, 879, 1756, 1894, 2167, 2215
BtsI GCAGTG 4 cut(s) 1042, 1054, 1173, 1578
BtsIMutI CAGTG 9 cut(s) 813, 1042, 1054, 1131, 1173, 1202, 1578, 1855, 2095
BveI ACCTGC 1 cut(s) 2275
Cac8I GCNNGC 2 cut(s) 1366, 2227
CaiI CAGNNNCTG 2 cut(s) 554, 1201
Cfr13I GGNCC 3 cut(s) 118, 557, 627
ClaI ATCGAT 1 cut(s) 264
CsiI ACCWGGT 2 cut(s) 169, 553
Csp6I GTAC 6 cut(s) 69, 205, 431, 609, 799, 1358
CviAII CATG 9 cut(s) 385, 686, 802, 838, 1170, 1670, 1717, 1829, 2226
CviQI GTAC 6 cut(s) 69, 205, 431, 609, 799, 1358
DdeI CTNAG 8 cut(s) 515, 734, 1245, 1290, 1501, 1620, 1702, 1768
DpnI GATC 8 cut(s) 128, 267, 450, 768, 879, 1008, 1218, 2355
DpnII GATC 8 cut(s) 126, 265, 448, 766, 877, 1006, 1216, 2353
DraI TTTAAA 1 cut(s) 714
DrdI GACNNNNNNGTC 1 cut(s) 1950
DseDI GACNNNNNNGTC 1 cut(s) 1950
Eam1104I CTCTTC 1 cut(s) 672
EarI CTCTTC 1 cut(s) 672
Eco130I CCWWGG 2 cut(s) 1669, 2172
Eco32I GATATC 2 cut(s) 1787, 1988
Eco47I GGWCC 3 cut(s) 118, 557, 627
Eco57I CTGAAG 1 cut(s) 2026
EcoNI CCTNNNNNAGG 2 cut(s) 793, 1829
EcoRI GAATTC 1 cut(s) 1060
EcoRII CCWGG 6 cut(s) 97, 136, 169, 472, 527, 553
EcoRV GATATC 2 cut(s) 1787, 1988
EcoT14I CCWWGG 2 cut(s) 1669, 2172
EcoT22I ATGCAT 1 cut(s) 390
ErhI CCWWGG 2 cut(s) 1669, 2172
Esp3I CGTCTC 2 cut(s) 138, 1405
FaeI CATG 9 cut(s) 388, 689, 805, 841, 1173, 1673, 1720, 1832, 2229
FaqI GGGAC 3 cut(s) 131, 543, 1990
FatI CATG 9 cut(s) 384, 685, 801, 837, 1169, 1669, 1716, 1828, 2225
FbaI TGATCA 1 cut(s) 448
Fnu4HI GCNGC 2 cut(s) 44, 470
FokI GGATG 8 cut(s) 265, 375, 438, 886, 1743, 1901, 2174, 2222
Fsp4HI GCNGC 2 cut(s) 44, 470
FspBI CTAG 6 cut(s) 9, 585, 962, 1454, 1745, 1991
GluI GCNGC 2 cut(s) 44, 470
GsaI CCCAGC 1 cut(s) 551
GsuI CTGGAG 1 cut(s) 550
HaeIII GGCC 2 cut(s) 102, 2272
HapII CCGG 2 cut(s) 853, 1106
Hin1II CATG 9 cut(s) 388, 689, 805, 841, 1173, 1673, 1720, 1832, 2229
HincII GTYRAC 1 cut(s) 345
HindII GTYRAC 1 cut(s) 345
HindIII AAGCTT 1 cut(s) 497
HinfI GANTC 9 cut(s) 88, 969, 1109, 1307, 1556, 1588, 1685, 1698, 1978
HpaII CCGG 2 cut(s) 853, 1106
HphI GGTGA 5 cut(s) 1226, 1613, 1636, 2024, 2326
Hpy166II GTNNAC 2 cut(s) 345, 2007
Hpy188I TCNGA 8 cut(s) 17, 616, 1203, 1291, 1406, 1504, 1962, 2347
Hpy188III TCNNGA 9 cut(s) 392, 668, 764, 962, 1106, 2076, 2131, 2252, 2300
Hpy8I GTNNAC 2 cut(s) 345, 2007
HpyAV CCTTC 8 cut(s) 959, 1288, 1511, 2073, 2087, 2257, 2297, 2371
HpyCH4III ACNGT 6 cut(s) 229, 1416, 1838, 1859, 2090, 2123
HpyCH4IV ACGT 1 cut(s) 131
HpyF10VI GCNNNNNNNGC 2 cut(s) 505, 749
HpyF3I CTNAG 8 cut(s) 515, 734, 1245, 1290, 1501, 1620, 1702, 1768
HpySE526I ACGT 1 cut(s) 131
Hsp92II CATG 9 cut(s) 388, 689, 805, 841, 1173, 1673, 1720, 1832, 2229
Kpn2I TCCGGA 1 cut(s) 1105
Ksp22I TGATCA 1 cut(s) 448
Kzo9I GATC 8 cut(s) 126, 265, 448, 766, 877, 1006, 1216, 2353
LmnI GCTCC 4 cut(s) 294, 854, 1520, 2306
Lsp1109I GCAGC 2 cut(s) 30, 456
LweI GCATC 7 cut(s) 21, 153, 207, 397, 431, 1377, 2351
MabI ACCWGGT 2 cut(s) 169, 553
MaeI CTAG 6 cut(s) 9, 585, 962, 1454, 1745, 1991
MaeII ACGT 1 cut(s) 131
MaeIII GTNAC 5 cut(s) 93, 173, 357, 1087, 1859
MalI GATC 8 cut(s) 128, 267, 450, 768, 879, 1008, 1218, 2355
MboI GATC 8 cut(s) 126, 265, 448, 766, 877, 1006, 1216, 2353
MflI RGATCY 1 cut(s) 877
MhlI GDGCHC 2 cut(s) 291, 1631
MlyI GAGTC 4 cut(s) 1103, 1597, 1692, 1972
MmeI TCCRAC 5 cut(s) 293, 479, 1333, 1669, 1866
Mph1103I ATGCAT 1 cut(s) 390
MroI TCCGGA 1 cut(s) 1105
MroXI GAANNNNTTC 1 cut(s) 653
MseI TTAA 7 cut(s) 314, 713, 1148, 1478, 1653, 1866, 2147
MslI CAYNNNNRTG 4 cut(s) 1014, 1047, 1571, 1999
MspA1I CMGCKG 1 cut(s) 633
MspI CCGG 2 cut(s) 853, 1106
MspR9I CCNGG 7 cut(s) 99, 138, 171, 474, 529, 555, 853
Mva1269I GAATGC 2 cut(s) 1067, 2247
MvaI CCWGG 6 cut(s) 99, 138, 171, 474, 529, 555
MwoI GCNNNNNNNGC 2 cut(s) 505, 749
NciI CCSGG 1 cut(s) 853
NcoI CCATGG 1 cut(s) 1669
NdeII GATC 8 cut(s) 126, 265, 448, 766, 877, 1006, 1216, 2353
NlaIII CATG 9 cut(s) 388, 689, 805, 841, 1173, 1673, 1720, 1832, 2229
NlaIV GGNNCC 4 cut(s) 119, 559, 850, 879
NmuCI GTSAC 2 cut(s) 357, 1859
NsiI ATGCAT 1 cut(s) 390
NspI RCATGY 3 cut(s) 689, 1720, 2229
NspV TTCGAA 1 cut(s) 972
OliI CACNNNNGTG 1 cut(s) 1047
PaeI GCATGC 1 cut(s) 2229
PctI GAATGC 2 cut(s) 1067, 2247
PdmI GAANNNNTTC 1 cut(s) 653
PfeI GAWTC 5 cut(s) 88, 969, 1307, 1556, 1685
PflMI CCANNNNNTGG 2 cut(s) 465, 554
PkrI GCNGC 2 cut(s) 45, 471
PleI GAGTC 4 cut(s) 1103, 1596, 1692, 1972
PpsI GAGTC 4 cut(s) 1103, 1596, 1692, 1972
PshAI GACNNNNGTC 1 cut(s) 1920
PshBI ATTAAT 1 cut(s) 1653
Psp6I CCWGG 6 cut(s) 97, 136, 169, 472, 527, 553
PspFI CCCAGC 1 cut(s) 547
PspGI CCWGG 6 cut(s) 97, 136, 169, 472, 527, 553
PspN4I GGNNCC 4 cut(s) 119, 559, 850, 879
PspPI GGNCC 3 cut(s) 118, 557, 627
PstI CTGCAG 2 cut(s) 430, 1049
PstNI CAGNNNCTG 2 cut(s) 554, 1201
PsuI RGATCY 1 cut(s) 877
RsaI GTAC 6 cut(s) 70, 206, 432, 610, 800, 1359
RsaNI GTAC 6 cut(s) 69, 205, 431, 609, 799, 1358
RseI CAYNNNNRTG 4 cut(s) 1014, 1047, 1571, 1999
SaqAI TTAA 7 cut(s) 314, 713, 1148, 1478, 1653, 1866, 2147
SatI GCNGC 2 cut(s) 44, 470
Sau3AI GATC 8 cut(s) 126, 265, 448, 766, 877, 1006, 1216, 2353
Sau96I GGNCC 3 cut(s) 118, 557, 627
ScaI AGTACT 3 cut(s) 432, 610, 1359
SchI GAGTC 4 cut(s) 1103, 1597, 1692, 1972
ScrFI CCNGG 7 cut(s) 99, 138, 171, 474, 529, 555, 853
SduI GDGCHC 2 cut(s) 291, 1631
SexAI ACCWGGT 2 cut(s) 169, 553
SfaNI GCATC 7 cut(s) 21, 153, 207, 397, 431, 1377, 2351
SfcI CTRYAG 7 cut(s) 378, 426, 1045, 1311, 1468, 1837, 2119
SfuI TTCGAA 1 cut(s) 972
SinI GGWCC 3 cut(s) 118, 557, 627
SmiMI CAYNNNNRTG 4 cut(s) 1014, 1047, 1571, 1999
SmlI CTYRAG 2 cut(s) 74, 1156
SmoI CTYRAG 2 cut(s) 74, 1156
SpeI ACTAGT 1 cut(s) 584
SphI GCATGC 1 cut(s) 2229
SsiI CCGC 1 cut(s) 633
SspMI CTAG 6 cut(s) 9, 585, 962, 1454, 1745, 1991
StyD4I CCNGG 7 cut(s) 97, 136, 169, 472, 527, 553, 851
StyI CCWWGG 2 cut(s) 1669, 2172
TaaI ACNGT 6 cut(s) 229, 1416, 1838, 1859, 2090, 2123
TaiI ACGT 1 cut(s) 134
TaqI TCGA 5 cut(s) 264, 885, 972, 1954, 2301
TatI WGTACW 4 cut(s) 68, 430, 608, 1357
TfiI GAWTC 5 cut(s) 88, 969, 1307, 1556, 1685
Tru1I TTAA 7 cut(s) 314, 713, 1148, 1478, 1653, 1866, 2147
Tru9I TTAA 7 cut(s) 314, 713, 1148, 1478, 1653, 1866, 2147
TscAI CASTG 9 cut(s) 820, 1049, 1054, 1138, 1180, 1202, 1578, 1862, 2095
TseFI GTSAC 2 cut(s) 357, 1859
TseI GCWGC 2 cut(s) 43, 469
Tsp45I GTSAC 2 cut(s) 357, 1859
TspDTI ATGAA 5 cut(s) 17, 839, 1412, 1905, 2226
TspRI CASTG 9 cut(s) 820, 1049, 1054, 1138, 1180, 1202, 1578, 1862, 2095
Van91I CCANNNNNTGG 2 cut(s) 465, 554
VpaK11BI GGWCC 3 cut(s) 118, 557, 627
VspI ATTAAT 1 cut(s) 1653
XagI CCTNNNNNAGG 2 cut(s) 793, 1829
XapI RAATTY 7 cut(s) 304, 349, 370, 862, 980, 1060, 2295
XbaI TCTAGA 1 cut(s) 961
XceI RCATGY 3 cut(s) 689, 1720, 2229
XcmI CCANNNNNNNNNTGG 2 cut(s) 110, 462
XmnI GAANNNNTTC 1 cut(s) 653
XspI CTAG 6 cut(s) 9, 585, 962, 1454, 1745, 1991
ZrmI AGTACT 3 cut(s) 432, 610, 1359
Zsp2I ATGCAT 1 cut(s) 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.