RLG00000018932

G-type lectin S-receptor-like serine threonine-protein kinase At2g19130

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000916
Physical Location & Seq
Reverse (-)
43043830 .. 43045504
1675 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018932

Sequence Viewer

Length: 753 bp
ATGCCAAATGGTTCTCTACAATCTATTTTAGTTGATAAGAAATCAATCATATTAGATTGGAAAGCAGGATATCGAATTGCAATAGGGACTGCCAAAGGATTGGCTTACCTTCATGATGAGTGTAGAGACTGCATCATACACTGTGACATTAAGCCTGAGAACATTTTGCTTGATGCAGAATACACTCCAAAAGTTGCAGATCTTGGTCTTGTAAAACCCATGGGCAGACACCATAGCCGGGTTCTGACAACAATGAGAGGAACTGCTAGCTATCTTGCTCCAGAATGGTTTACAGTTGAAGCCATCAAGCCAAAGGCCGATGTCTTCAGCTATGGAAGGTTGGGAGGTAATGTTGACAAAGATGAACTCGATACAGCATGCAAAGTCTCGTGCTGGTGCAGTCGAGATGATGAGAAGGATAGACCAACTATGAGGCAGGTTGTTCAAATTCTTGAGGGAGTTTTAGATGTTGGTATACCCCCTATTCCACAGTTCCTCCAGCGCCTTACTACCTCCTCCACCGTCTTCGGCCTCGGCAGAGGCAATGCTGTTGTAGACGTTGCGGAAAAAGTCGTCGACTGTCATCGAGCCCAACTAGATCGGCTTGGGATTGGCCGTGTTAATGTAGCTGGTGGTGGTGGTGTGGTGTTTTGGGTTGAAAGGCAAGAAATCAAAGACATCGAAGCCAATAAGGAGGAAAAGTTGAGTACGGTTGAAAAGTGCTCGCCGGAGCGATTGGAGAAGAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_2 Pkinase S_locus_glycop

Protein Analysis

251

Amino Acids

27.72

Weight (kDa)

6.95

Isoelectric Point (pI)

31.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 1 - 112 1.7e-22 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 1 - 112 1e-14 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 427
AccI GTMKAC 3 cut(s) 475, 555, 576
AciI CCGC 1 cut(s) 563
AcoI YGGCCR 1 cut(s) 613
AcsI RAATTY 1 cut(s) 447
AcuI CTGAAG 1 cut(s) 310
AfaI GTAC 1 cut(s) 709
AfiI CCNNNNNNNGG 1 cut(s) 238
AgsI TTSAA 4 cut(s) 299, 446, 659, 716
AluBI AGCT 3 cut(s) 270, 330, 629
AluI AGCT 3 cut(s) 270, 330, 629
Alw21I GWGCWC 1 cut(s) 725
Alw26I GTCTC 2 cut(s) 120, 391
AoxI GGCC 3 cut(s) 315, 529, 613
ApoI RAATTY 1 cut(s) 447
AspLEI GCGC 1 cut(s) 504
AsuC2I CCSGG 1 cut(s) 239
AsuNHI GCTAGC 1 cut(s) 266
BanII GRGCYC 1 cut(s) 592
BauI CACGAG 1 cut(s) 388
BbsI GAAGAC 2 cut(s) 316, 517
Bbv12I GWGCWC 1 cut(s) 725
BccI CCATC 1 cut(s) 311
BceAI ACGGC 1 cut(s) 600
BcnI CCSGG 1 cut(s) 239
BcoDI GTCTC 2 cut(s) 120, 391
BfaI CTAG 2 cut(s) 267, 596
BfoI RGCGCY 1 cut(s) 505
BfuAI ACCTGC 1 cut(s) 427
BglII AGATCT 1 cut(s) 199
Bme1390I CCNGG 1 cut(s) 239
BmrFI CCNGG 1 cut(s) 239
BmsI GCATC 2 cut(s) 141, 163
BmtI GCTAGC 1 cut(s) 270
BpiI GAAGAC 2 cut(s) 316, 517
BpmI CTGGAG 2 cut(s) 264, 482
BpuEI CTTGAG 1 cut(s) 473
BpuMI CCSGG 1 cut(s) 239
BsaJI CCNNGG 2 cut(s) 219, 532
BsaXI ACNNNNNCTCC 6 cut(s) 336, 366, 480, 510, 686, 716
Bsc4I CCNNNNNNNGG 1 cut(s) 238
Bse3DI GCAATG 1 cut(s) 550
BseDI CCNNGG 2 cut(s) 219, 532
BseLI CCNNNNNNNGG 1 cut(s) 238
BseMI GCAATG 1 cut(s) 550
BseMII CTCAG 1 cut(s) 147
BseRI GAGGAG 1 cut(s) 505
BsgI GTGCAG 1 cut(s) 418
BshFI GGCC 3 cut(s) 317, 531, 615
BsiHKAI GWGCWC 1 cut(s) 725
BsiSI CCGG 2 cut(s) 238, 728
BslFI GGGAC 1 cut(s) 100
BslI CCNNNNNNNGG 1 cut(s) 238
BsmAI GTCTC 2 cut(s) 120, 391
BsmFI GGGAC 1 cut(s) 100
BsnI GGCC 3 cut(s) 317, 531, 615
Bsp1286I GDGCHC 2 cut(s) 592, 725
Bsp143I GATC 2 cut(s) 199, 598
Bsp19I CCATGG 1 cut(s) 219
BspACI CCGC 1 cut(s) 563
BspANI GGCC 3 cut(s) 317, 531, 615
BspCNI CTCAG 1 cut(s) 148
BspHI TCATGA 1 cut(s) 112
BspMI ACCTGC 1 cut(s) 427
BspOI GCTAGC 1 cut(s) 270
BsrDI GCAATG 1 cut(s) 550
BssECI CCNNGG 2 cut(s) 219, 532
BssMI GATC 2 cut(s) 199, 598
BssNAI GTATAC 1 cut(s) 476
BssSI CACGAG 1 cut(s) 388
BssT1I CCWWGG 1 cut(s) 219
Bst1107I GTATAC 1 cut(s) 476
Bst2BI CACGAG 1 cut(s) 388
Bst4CI ACNGT 6 cut(s) 143, 295, 492, 523, 581, 712
BstC8I GCNNGC 3 cut(s) 268, 379, 725
BstDEI CTNAG 1 cut(s) 156
BstDSI CCRYGG 1 cut(s) 219
BstH2I RGCGCY 1 cut(s) 505
BstHHI GCGC 1 cut(s) 504
BstKTI GATC 2 cut(s) 202, 601
BstMAI GTCTC 2 cut(s) 120, 391
BstMBI GATC 2 cut(s) 199, 598
BstNSI RCATGY 1 cut(s) 381
BstSCI CCNGG 1 cut(s) 237
BstV2I GAAGAC 2 cut(s) 316, 517
BstX2I RGATCY 1 cut(s) 199
BstXI CCANNNNNNTGG 1 cut(s) 100
BstYI RGATCY 1 cut(s) 199
BstZ17I GTATAC 1 cut(s) 476
BsuRI GGCC 3 cut(s) 317, 531, 615
BtgI CCRYGG 1 cut(s) 219
BtsIMutI CAGTG 1 cut(s) 139
BveI ACCTGC 1 cut(s) 427
Cac8I GCNNGC 3 cut(s) 268, 379, 725
CciI TCATGA 1 cut(s) 112
CfoI GCGC 1 cut(s) 504
Csp6I GTAC 1 cut(s) 708
CviAII CATG 3 cut(s) 113, 220, 378
CviQI GTAC 1 cut(s) 708
DdeI CTNAG 1 cut(s) 156
DpnI GATC 2 cut(s) 201, 600
DpnII GATC 2 cut(s) 199, 598
EaeI YGGCCR 1 cut(s) 613
Eco130I CCWWGG 1 cut(s) 219
Eco24I GRGCYC 1 cut(s) 592
Eco32I GATATC 1 cut(s) 71
Eco57I CTGAAG 1 cut(s) 310
EcoRV GATATC 1 cut(s) 71
EcoT14I CCWWGG 1 cut(s) 219
EcoT38I GRGCYC 1 cut(s) 592
ErhI CCWWGG 1 cut(s) 219
FaeI CATG 3 cut(s) 116, 223, 381
FaiI YATR 9 cut(s) 50, 114, 137, 221, 234, 333, 379, 431, 476
FaqI GGGAC 1 cut(s) 100
FatI CATG 3 cut(s) 112, 219, 377
FblI GTMKAC 3 cut(s) 475, 555, 576
FriOI GRGCYC 1 cut(s) 592
FspBI CTAG 2 cut(s) 267, 596
GlaI GCGC 1 cut(s) 503
GsuI CTGGAG 2 cut(s) 264, 482
HaeII RGCGCY 1 cut(s) 505
HaeIII GGCC 3 cut(s) 317, 531, 615
HapII CCGG 2 cut(s) 238, 728
HhaI GCGC 1 cut(s) 504
Hin1II CATG 3 cut(s) 116, 223, 381
Hin6I GCGC 1 cut(s) 502
HinP1I GCGC 1 cut(s) 502
HincII GTYRAC 2 cut(s) 355, 577
HindII GTYRAC 2 cut(s) 355, 577
HpaII CCGG 2 cut(s) 238, 728
Hpy166II GTNNAC 5 cut(s) 291, 355, 476, 556, 577
Hpy188I TCNGA 1 cut(s) 246
Hpy188III TCNNGA 4 cut(s) 113, 281, 404, 452
Hpy8I GTNNAC 5 cut(s) 291, 355, 476, 556, 577
Hpy99I CGWCG 1 cut(s) 578
HpyAV CCTTC 3 cut(s) 119, 330, 409
HpyCH4III ACNGT 6 cut(s) 143, 295, 492, 523, 581, 712
HpyCH4IV ACGT 1 cut(s) 558
HpyCH4V TGCA 6 cut(s) 80, 132, 176, 197, 381, 399
HpyF3I CTNAG 1 cut(s) 156
HpySE526I ACGT 1 cut(s) 558
Hsp92II CATG 3 cut(s) 116, 223, 381
HspAI GCGC 1 cut(s) 502
Kzo9I GATC 2 cut(s) 199, 598
LmnI GCTCC 2 cut(s) 283, 730
LpnPI CCDG 9 cut(s) 51, 168, 251, 294, 379, 422, 512, 615, 741
LweI GCATC 2 cut(s) 141, 163
MaeI CTAG 2 cut(s) 267, 596
MaeII ACGT 1 cut(s) 558
MaeIII GTNAC 1 cut(s) 143
MalI GATC 2 cut(s) 201, 600
MboI GATC 2 cut(s) 199, 598
MboII GAAGA 2 cut(s) 316, 517
MflI RGATCY 1 cut(s) 199
MhlI GDGCHC 2 cut(s) 592, 725
MluCI AATT 2 cut(s) 75, 447
MseI TTAA 2 cut(s) 150, 621
MspI CCGG 2 cut(s) 238, 728
MspR9I CCNGG 1 cut(s) 239
NciI CCSGG 1 cut(s) 239
NcoI CCATGG 1 cut(s) 219
NdeII GATC 2 cut(s) 199, 598
NheI GCTAGC 1 cut(s) 266
NlaIII CATG 3 cut(s) 116, 223, 381
NmeAIII GCCGAG 1 cut(s) 513
NmuCI GTSAC 1 cut(s) 143
NspI RCATGY 1 cut(s) 381
PaeI GCATGC 1 cut(s) 381
PagI TCATGA 1 cut(s) 112
PsuI RGATCY 1 cut(s) 199
RsaI GTAC 1 cut(s) 709
RsaNI GTAC 1 cut(s) 708
SalI GTCGAC 1 cut(s) 575
SaqAI TTAA 2 cut(s) 150, 621
Sau3AI GATC 2 cut(s) 199, 598
ScrFI CCNGG 1 cut(s) 239
SduI GDGCHC 2 cut(s) 592, 725
SetI ASST 9 cut(s) 111, 272, 332, 341, 349, 441, 515, 561, 631
SfaNI GCATC 2 cut(s) 141, 163
SmlI CTYRAG 1 cut(s) 452
SmoI CTYRAG 1 cut(s) 452
SphI GCATGC 1 cut(s) 381
Sse9I AATT 2 cut(s) 75, 447
SsiI CCGC 1 cut(s) 563
SspMI CTAG 2 cut(s) 267, 596
StyD4I CCNGG 1 cut(s) 237
StyI CCWWGG 1 cut(s) 219
TaaI ACNGT 6 cut(s) 143, 295, 492, 523, 581, 712
TaiI ACGT 1 cut(s) 561
TaqI TCGA 6 cut(s) 73, 369, 403, 576, 586, 681
TasI AATT 2 cut(s) 75, 447
Tru1I TTAA 2 cut(s) 150, 621
Tru9I TTAA 2 cut(s) 150, 621
TscAI CASTG 1 cut(s) 146
TseFI GTSAC 1 cut(s) 143
Tsp45I GTSAC 1 cut(s) 143
TspDTI ATGAA 2 cut(s) 101, 378
TspRI CASTG 1 cut(s) 146
XapI RAATTY 1 cut(s) 447
XceI RCATGY 1 cut(s) 381
XmiI GTMKAC 3 cut(s) 475, 555, 576
XspI CTAG 2 cut(s) 267, 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.