Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0000916

Physical Location & Seq

Genomic Coordinates Forward (+)

44115466 .. 44117889

Gene Structure 2424 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00116840.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2424 bp

ATGATTCATTTTGGTTCAGAGTGTGTGCTTGTTGTGTTATGTTGTTTCATCATCATCAGCTTCGGAGCTCATCTCTCAACGGCGTCTGACAAGCTCATCCCAGGCCAGTCACTTTCCGGCAACCAGACATTAACCTCTCCGGCAGGCAAATTTGAACTGGGTTTCTTCACTCGAGGTAACTCTAACAATTACTACATAGGCATTTGGTACGCAAATCTGCCTGATCATACAGTTGTCTGGGTGGCTAATAGAAACCACCCTGTTTCTGATCCAGTTTCTTCAGAGTTTAGATTCCTTGAAAATGGAAACTTTACCTTGCTGGACCAGTCCAAATCTTCAATTTGGTCAACCCAGAATTCTATGTCTTTAATGTCCAAATCCGCCACATATACAGCAAGGCTTCTTGACAATGGCAACTTTGTTATTCAAGATGCATCTGGTGTAATATGGCAGAGTTTTGACCACCCAACTGATACTTGGCTCCCAGGTGGTGCCAGGCTTGGTTACAACAAGCTTACCAATCAGAAACTAGCTCTCACTCCCTGGAAAAACCCACAAGACCCAGCCCCTGGCATTTTTTCCCTTGAGATTGAACCAAATGGGACAAGTTTTTGGTTGCTCTATAACGGCTCTCAACAGTATTGGAATAGTGGGCCTTGGAATGGCAAATCTTTTGCCCAAGTCCCTGAAATAGAGCGGAACGATCATATAACAAATGTTAGTTTTGTTTCAGATGACAAGGGGAGCTATTTTTCTTATGATTCTGCAGACCCTGACACTTATATTAGGTACATGATTGACGTCACTGGGCAGTTCAAGGTCTATATGTGGGGGAAGAACTTCACGCAGTGGAATTCATTTTGGTTGAGGCCAACCCAATTCTGTGAGGTTTATGGATTTTGTGGTGCTTCTAGCATCTGCCAGCAAAACGCCAGTCTGTGTGTTTGTATGGAGGGATATGAACCTAAAGTCCCAAAAGAGTGGGGATTAGGGGATCACACAGATGGGTGTGTCAGGAAAGCACATTTACAATGCATTACTGGAGTAGATGATACTTTTAAGGTGGTATCAGATGTCCGCCTTCCTGTGAATCCAGAGACTTTAGCAGTTGATGACAGTGATGAGTGCAGATTAGCGTGCTTAAGAAATTGCTCATGTACAGCTTTTGCTTATGATAATGGGTGTCATGTTTGGGCAGGGGATCTATTCAACGTCAAACAATTTACACCAGACGAGAAGGAAGGCAAGGACTTGCATCTGAAAATTCCAGCTTCTGAGAGAATAGAGGGGAAGAAAGAGAACAGGACTCTTTGGATCATCATCGGAGTACTTGGAGGTTTATTTATTGTTATTACCATTGTTGTGGTACTATTTGTGAAGCATCGATGTTTTGGGGAAAAGTTAGATACACTTGATGGTTCCTTGGTGATGTTCAGGTATAGGGTTTTAAGAAAGGCAACAAACAACTTTTCGGAGAAACTTGGGGAAGGTGGCTTTGGTTCTGTTTTCAAAGGGACATTGCAGGGATCAATTGCCATTGCAGTGAAGAGACTCAACTGTCCCAAGCAAGAGGATAAGCAATTCCTTACAGAAGTGAGAACTATTGGAAAAGTCCAACACATTAATCTTATTCGTCTTTGTGGATTTTGTGCAGAAGCCTCAAAAAGATTCTTGGTTTACGAATACATGCCAAATGGGTCTCTAGAATCTGTTCTATTTAAAAACAGTTCCATTGTCTTAGATTGGAAAGCTAGGTACCAAATTGCAATTGGGACTGCTAGAGGATTGGCCTACCTTCATGAGGAATGTAGAGAATGCATAATACACTGCGATATCAAACCTGAAAACATTCTCTTGGATGCAGAATATGTCCCAAAAGTAGCAGATTTTGGTCTAGCAAAGCTCATAGACAGAGACATTAGCCGGATAATAACAACCATGAAAGGAACCAGAGGCTATATTGCTCCAGAGTGGATTTCAGGAGAAGCTATAACATCAAAAGCTGATACCTTTAGCTATGGAATGCTGTTATTTGAACTTATCTCGGGAAGGAGAAACAGAGATCTATTAGATGATGCTTTAGAAAATTACTTCCCAACCCGTGTTGCCAATGTGTTGAATAAAGAAGAAGATGTTGATGCCTTGTTGGATTACAGGTTAGAAGGTAATGCTGACAAAGAACAACTGAGTAGAGCATGCAAAGTCGCTTGTTGGTGCATTCAAGACGATGAGAAGGGTAGGCCAACTATGGGGCAGGTTGTTCAACTTCTGGAGGGAGTTATAGATGCTGGAATTCCACCAATGCCAAAGTTCCTCGACCGCTTTTCTAAGAGTCTAGTTGAATCCATCAATTATCGGTACAGTACTTGCAGCAGTTCTGATTCATGTCGTCATGAGTACTCAAATAGTATATATCAAACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

B_lectin PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

807

Amino Acids

90.75

Weight (kDa)

5.78

Isoelectric Point (pI)

31.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	77 - 183	7.8e-29	D-mannose binding lectin
S_locus_glycop	PF00954	215 - 323	2.9e-23	S-locus glycoprotein domain
PAN_2	PF08276	347 - 405	1.1e-13	PAN-like domain
PK_Tyr_Ser-Thr	PF07714	490 - 756	3e-43	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	491 - 755	2.5e-49	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0000916)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g26420 FvH4_6g26420 FvH4_6g26450 FvH4_6g26451
malus_domestica	MD09G1116700.v1.1 MD09G1233200.v1.1 MD12G1033500.v1.1 MD14G1034400.v1.1 MD14G1034600.v1.1 MD14G1034700.v1.1 MD14G1034800.v1.1 MD14G1034900.v1.1 MD14G1035200.v1.1 MD17G1090300.v1.1 MD17G1108200.v1.1 MD17G1237700.v1.1 MD17G1237800.v1.1 MD17G1238000.v1.1 MD17G1238400.v1.1
prunus_persica	Prupe.3G131100_v2.0.a1 Prupe.3G141400_v2.0.a1
pyrus_communis	pycom09g04120 pycom12g03080 pycom14g03020 pycom14g03080 pycom14g03090 pycom17g24250
rosa_chinensis	RchiOBHm_Chr2g0127791 RchiOBHm_Chr2g0127811 RchiOBHm_Chr2g0127821 RchiOBHm_Chr2g0128001
rosa_laevigata	RLG00000002113 RLG00000018931 RLG00000018932 RLG00000018933 RLG00000018934 RLG00000023426
rosa_multiflora	Rmu_sc0000516.1_g000026 Rmu_sc0000516.1_g000045 Rmu_ssc0000093.1_g000010 Rmu_ssc0000093.1_g000016 Rmu_ssc0000093.1_g000017 Rmu_ssc0000093.1_g000024
rosa_roxburghii	Rroxscaffold_2G00116790 Rroxscaffold_2G00116800 Rroxscaffold_2G00116810 Rroxscaffold_2G00116840
rosa_rugosa	Rorug02G0270500 Rorug02G0270600 Rorug02G0270800 Rorug02G0270900 Rorug02G0271000
rosa_samantha	Rh2AG323100 Rh2BG332100 Rh2BG332200 Rh2BG332300 Rh2CG310900 Rh2CG311000 Rh2DG350400 Rh2DG350500 Rh2DG350600 Rh2DG350700
rosa_wichuraiana	Rw2G026190 Rw2G026210 Rw2G026230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1557
AatII	GACGTC	1 cut(s)	804
Acc36I	ACCTGC	1 cut(s)	2245
Acc65I	GGTACC	1 cut(s)	1755
AccB1I	GGYRCC	2 cut(s)	491, 1755
AccB7I	CCANNNNNTGG	1 cut(s)	569
AccBSI	CCGCTC	1 cut(s)	697
AciI	CCGC	4 cut(s)	381, 697, 1078, 2320
AclWI	GGATC	5 cut(s)	263, 1002, 1209, 1322, 1534
AcsI	RAATTY	5 cut(s)	149, 355, 853, 1263, 2292
AcuI	CTGAAG	1 cut(s)	264
AcyI	GRCGYC	2 cut(s)	83, 801
AdeI	CACNNNGTG	1 cut(s)	849
AfaI	GTAC	9 cut(s)	209, 791, 1159, 1329, 1368, 1757, 2360, 2365, 2399
AfiI	CCNNNNNNNGG	4 cut(s)	569, 662, 1801, 2249
AflII	CTTAAG	1 cut(s)	1141
AjnI	CCWGG	5 cut(s)	100, 484, 494, 542, 568
AjuI	GAANNNNNNNTTGG	2 cut(s)	1479, 1511
AloI	GAACNNNNNNTCC	2 cut(s)	954, 986
Alw21I	GWGCWC	1 cut(s)	70
Alw26I	GTCTC	4 cut(s)	1091, 1543, 1704, 1908
AlwI	GGATC	5 cut(s)	263, 1002, 1209, 1322, 1534
AlwNI	CAGNNNCTG	3 cut(s)	569, 773, 1274
Ama87I	CYCGRG	2 cut(s)	171, 2044
AoxI	GGCC	5 cut(s)	103, 653, 869, 1788, 2240
ApeKI	GCWGC	1 cut(s)	2370
ApoI	RAATTY	5 cut(s)	149, 355, 853, 1263, 2292
ArsI	GACNNNNNNTTYG	2 cut(s)	919, 951
AseI	ATTAAT	1 cut(s)	1623
Asp700I	GAANNNNTTC	3 cut(s)	839, 1710, 1848
Asp718I	GGTACC	1 cut(s)	1755
AspS9I	GGNCC	2 cut(s)	322, 653
AsuHPI	GGTGA	1 cut(s)	1438
AvaI	CYCGRG	2 cut(s)	171, 2044
AvaII	GGWCC	1 cut(s)	322
BanI	GGYRCC	2 cut(s)	491, 1755
BanII	GRGCYC	1 cut(s)	70
Bbv12I	GWGCWC	1 cut(s)	70
BbvI	GCAGC	1 cut(s)	2382
BccI	CCATC	3 cut(s)	998, 1409, 2354
BceAI	ACGGC	2 cut(s)	96, 643
BciT130I	CCWGG	5 cut(s)	102, 486, 496, 544, 570
BclI	TGATCA	1 cut(s)	223
BcoDI	GTCTC	4 cut(s)	1091, 1543, 1704, 1908
BfaI	CTAG	7 cut(s)	530, 912, 1703, 1752, 1779, 1895, 2336
BfmI	CTRYAG	1 cut(s)	765
BfrI	CTTAAG	1 cut(s)	1141
BfuAI	ACCTGC	1 cut(s)	2245
BglII	AGATCT	1 cut(s)	2062
BisI	GCNGC	1 cut(s)	2371
BlsI	GCNGC	1 cut(s)	2372
BmcAI	AGTACT	3 cut(s)	1329, 2365, 2399
Bme1390I	CCNGG	5 cut(s)	102, 486, 496, 544, 570
Bme18I	GGWCC	1 cut(s)	322
BmeT110I	CYCGRG	2 cut(s)	171, 2044
BmgT120I	GGNCC	2 cut(s)	322, 653
BmiI	GGNNCC	5 cut(s)	482, 493, 1420, 1757, 1948
BmrFI	CCNGG	5 cut(s)	102, 486, 496, 544, 570
BmrI	ACTGGG	2 cut(s)	167, 816
BmsI	GCATC	9 cut(s)	421, 443, 924, 1264, 1390, 1849, 2065, 2128, 2275
BmuI	ACTGGG	2 cut(s)	167, 816
BplI	GAGNNNNNCTC	2 cut(s)	57, 89
BpmI	CTGGAG	3 cut(s)	1062, 1950, 2291
BpuEI	CTTGAG	1 cut(s)	605
Bsa29I	ATCGAT	1 cut(s)	1384
BsaBI	GATNNNNATC	1 cut(s)	1319
BsaHI	GRCGYC	2 cut(s)	83, 801
BsaI	GGTCTC	1 cut(s)	1704
BsaJI	CCNNGG	6 cut(s)	100, 484, 542, 568, 656, 1422
Bsc4I	CCNNNNNNNGG	4 cut(s)	569, 662, 1801, 2249
Bse1I	ACTGG	7 cut(s)	106, 162, 272, 325, 811, 933, 1045
Bse3DI	GCAATG	2 cut(s)	1517, 1536
Bse8I	GATNNNNATC	1 cut(s)	1319
BseBI	CCWGG	5 cut(s)	102, 486, 496, 544, 570
BseCI	ATCGAT	1 cut(s)	1384
BseDI	CCNNGG	6 cut(s)	100, 484, 542, 568, 656, 1422
BseGI	GGATG	2 cut(s)	96, 1864
BseJI	GATNNNNATC	1 cut(s)	1319
BseLI	CCNNNNNNNGG	4 cut(s)	569, 662, 1801, 2249
BseMI	GCAATG	2 cut(s)	1517, 1536
BseMII	CTCAG	2 cut(s)	1266, 2177
BseNI	ACTGG	7 cut(s)	106, 162, 272, 325, 811, 933, 1045
BseXI	GCAGC	1 cut(s)	2382
BseYI	CCCAGC	1 cut(s)	562
BsgI	GTGCAG	2 cut(s)	1147, 1671
Bsh1285I	CGRYCG	1 cut(s)	2320
BshFI	GGCC	5 cut(s)	105, 655, 871, 1790, 2242
BshNI	GGYRCC	2 cut(s)	491, 1755
BshVI	ATCGAT	1 cut(s)	1384
BsiEI	CGRYCG	1 cut(s)	2320
BsiHKAI	GWGCWC	1 cut(s)	70
BsiHKCI	CYCGRG	2 cut(s)	171, 2044
BsiSI	CCGG	3 cut(s)	117, 140, 1924
BslFI	GGGAC	7 cut(s)	616, 668, 956, 1528, 1545, 1786, 1856
BslI	CCNNNNNNNGG	4 cut(s)	569, 662, 1801, 2249
BsmAI	GTCTC	4 cut(s)	1091, 1543, 1704, 1908
BsmFI	GGGAC	7 cut(s)	616, 668, 956, 1528, 1545, 1786, 1856
BsmI	GAATGC	3 cut(s)	1820, 2028, 2217
BsnI	GGCC	5 cut(s)	105, 655, 871, 1790, 2242
Bso31I	GGTCTC	1 cut(s)	1704
BsoBI	CYCGRG	2 cut(s)	171, 2044
Bsp1286I	GDGCHC	1 cut(s)	70
Bsp1407I	TGTACA	1 cut(s)	1157
Bsp143I	GATC	8 cut(s)	223, 268, 703, 994, 1201, 1314, 1526, 2062
BspACI	CCGC	4 cut(s)	381, 697, 1078, 2320
BspANI	GGCC	5 cut(s)	105, 655, 871, 1790, 2242
BspCNI	CTCAG	2 cut(s)	1267, 2178
BspDI	ATCGAT	1 cut(s)	1384
BspHI	TCATGA	2 cut(s)	1798, 2392
BspLI	GGNNCC	5 cut(s)	482, 493, 1420, 1757, 1948
BspMAI	CTGCAG	1 cut(s)	769
BspMI	ACCTGC	1 cut(s)	2245
BspPI	GGATC	5 cut(s)	263, 1002, 1209, 1322, 1534
BspT107I	GGYRCC	2 cut(s)	491, 1755
BspTI	CTTAAG	1 cut(s)	1141
BspTNI	GGTCTC	1 cut(s)	1704
BsrBI	CCGCTC	1 cut(s)	697
BsrDI	GCAATG	2 cut(s)	1517, 1536
BsrGI	TGTACA	1 cut(s)	1157
BsrI	ACTGG	7 cut(s)	106, 162, 272, 325, 811, 933, 1045
BssECI	CCNNGG	6 cut(s)	100, 484, 542, 568, 656, 1422
BssMI	GATC	8 cut(s)	223, 268, 703, 994, 1201, 1314, 1526, 2062
BssNI	GRCGYC	2 cut(s)	83, 801
BssT1I	CCWWGG	2 cut(s)	656, 1422
Bst2UI	CCWGG	5 cut(s)	102, 486, 496, 544, 570
Bst4CI	ACNGT	6 cut(s)	232, 639, 1118, 1559, 1727, 2363
Bst6I	CTCTTC	1 cut(s)	1541
BstACI	GRCGYC	2 cut(s)	83, 801
BstAFI	CTTAAG	1 cut(s)	1141
BstAUI	TGTACA	1 cut(s)	1157
BstC8I	GCNNGC	4 cut(s)	145, 923, 1138, 2197
BstDEI	CTNAG	4 cut(s)	1275, 1738, 2186, 2328
BstENI	CCTNNNNNAGG	1 cut(s)	1799
BstF5I	GGATG	2 cut(s)	96, 1864
BstKTI	GATC	8 cut(s)	226, 271, 706, 997, 1204, 1317, 1529, 2065
BstMAI	GTCTC	4 cut(s)	1091, 1543, 1704, 1908
BstMBI	GATC	8 cut(s)	223, 268, 703, 994, 1201, 1314, 1526, 2062
BstMCI	CGRYCG	1 cut(s)	2320
BstNI	CCWGG	5 cut(s)	102, 486, 496, 544, 570
BstNSI	RCATGY	2 cut(s)	1690, 2199
BstSCI	CCNGG	5 cut(s)	100, 484, 494, 542, 568
BstSFI	CTRYAG	1 cut(s)	765
BstV1I	GCAGC	1 cut(s)	2382
BstX2I	RGATCY	2 cut(s)	1201, 2062
BstXI	CCANNNNNNTGG	2 cut(s)	981, 1363
BstYI	RGATCY	2 cut(s)	1201, 2062
Bsu15I	ATCGAT	1 cut(s)	1384
BsuRI	GGCC	5 cut(s)	105, 655, 871, 1790, 2242
BsuTUI	ATCGAT	1 cut(s)	1384
BtsCI	GGATG	2 cut(s)	96, 1864
BtsI	GCAGTG	3 cut(s)	854, 1548, 1825
BtsIMutI	CAGTG	5 cut(s)	804, 854, 1123, 1548, 1825
BveI	ACCTGC	1 cut(s)	2245
Cac8I	GCNNGC	4 cut(s)	145, 923, 1138, 2197
CaiI	CAGNNNCTG	3 cut(s)	569, 773, 1274
CciI	TCATGA	2 cut(s)	1798, 2392
Cfr13I	GGNCC	2 cut(s)	322, 653
ClaI	ATCGAT	1 cut(s)	1384
CseI	GACGC	1 cut(s)	72
Csp6I	GTAC	9 cut(s)	208, 790, 1158, 1328, 1367, 1756, 2359, 2364, 2398
CviAII	CATG	9 cut(s)	793, 1155, 1187, 1687, 1799, 1939, 2196, 2385, 2393
CviQI	GTAC	9 cut(s)	208, 790, 1158, 1328, 1367, 1756, 2359, 2364, 2398
DdeI	CTNAG	4 cut(s)	1275, 1738, 2186, 2328
DpnI	GATC	8 cut(s)	225, 270, 705, 996, 1203, 1316, 1528, 2064
DpnII	GATC	8 cut(s)	223, 268, 703, 994, 1201, 1314, 1526, 2062
DraI	TTTAAA	1 cut(s)	1720
DraIII	CACNNNGTG	1 cut(s)	849
DrdI	GACNNNNNNGTC	1 cut(s)	1557
DseDI	GACNNNNNNGTC	1 cut(s)	1557
Eam1104I	CTCTTC	1 cut(s)	1541
EarI	CTCTTC	1 cut(s)	1541
EciI	GGCGGA	2 cut(s)	370, 1067
Ecl136II	GAGCTC	1 cut(s)	68
Eco130I	CCWWGG	2 cut(s)	656, 1422
Eco24I	GRGCYC	1 cut(s)	70
Eco31I	GGTCTC	1 cut(s)	1704
Eco32I	GATATC	1 cut(s)	1834
Eco47I	GGWCC	1 cut(s)	322
Eco53kI	GAGCTC	1 cut(s)	68
Eco57I	CTGAAG	1 cut(s)	264
Eco88I	CYCGRG	2 cut(s)	171, 2044
EcoICRI	GAGCTC	1 cut(s)	68
EcoNI	CCTNNNNNAGG	1 cut(s)	1799
EcoRI	GAATTC	3 cut(s)	355, 853, 2292
EcoRII	CCWGG	5 cut(s)	100, 484, 494, 542, 568
EcoRV	GATATC	1 cut(s)	1834
EcoT14I	CCWWGG	2 cut(s)	656, 1422
EcoT22I	ATGCAT	3 cut(s)	436, 1037, 1820
EcoT38I	GRGCYC	1 cut(s)	70
ErhI	CCWWGG	2 cut(s)	656, 1422
FaeI	CATG	9 cut(s)	796, 1158, 1190, 1690, 1802, 1942, 2199, 2388, 2396
FaqI	GGGAC	7 cut(s)	616, 668, 956, 1528, 1545, 1786, 1856
FatI	CATG	9 cut(s)	792, 1154, 1186, 1686, 1798, 1938, 2195, 2384, 2392
FbaI	TGATCA	1 cut(s)	223
Fnu4HI	GCNGC	1 cut(s)	2371
FokI	GGATG	2 cut(s)	83, 1871
FriOI	GRGCYC	1 cut(s)	70
Fsp4HI	GCNGC	1 cut(s)	2371
FspBI	CTAG	7 cut(s)	530, 912, 1703, 1752, 1779, 1895, 2336
GluI	GCNGC	1 cut(s)	2371
GsaI	CCCAGC	1 cut(s)	566
GsuI	CTGGAG	3 cut(s)	1062, 1950, 2291
HaeIII	GGCC	5 cut(s)	105, 655, 871, 1790, 2242
HapII	CCGG	3 cut(s)	117, 140, 1924
HgaI	GACGC	1 cut(s)	72
Hin1I	GRCGYC	2 cut(s)	83, 801
Hin1II	CATG	9 cut(s)	796, 1158, 1190, 1690, 1802, 1942, 2199, 2388, 2396
HincII	GTYRAC	1 cut(s)	348
HindII	GTYRAC	1 cut(s)	348
HindIII	AAGCTT	1 cut(s)	512
HpaII	CCGG	3 cut(s)	117, 140, 1924
HphI	GGTGA	1 cut(s)	1438
Hpy166II	GTNNAC	2 cut(s)	348, 1678
Hpy8I	GTNNAC	2 cut(s)	348, 1678
HpyAV	CCTTC	8 cut(s)	1091, 1231, 1235, 1481, 1805, 2043, 2156, 2227
HpyCH4III	ACNGT	6 cut(s)	232, 639, 1118, 1559, 1727, 2363
HpyCH4IV	ACGT	2 cut(s)	801, 1212
HpyF3I	CTNAG	4 cut(s)	1275, 1738, 2186, 2328
HpySE526I	ACGT	2 cut(s)	801, 1212
Hsp92I	GRCGYC	2 cut(s)	83, 801
Hsp92II	CATG	9 cut(s)	796, 1158, 1190, 1690, 1802, 1942, 2199, 2388, 2396
KpnI	GGTACC	1 cut(s)	1759
Ksp22I	TGATCA	1 cut(s)	223
Kzo9I	GATC	8 cut(s)	223, 268, 703, 994, 1201, 1314, 1526, 2062
LmnI	GCTCC	4 cut(s)	65, 486, 744, 1969
Lsp1109I	GCAGC	1 cut(s)	2382
LweI	GCATC	9 cut(s)	421, 443, 924, 1264, 1390, 1849, 2065, 2128, 2275
MaeI	CTAG	7 cut(s)	530, 912, 1703, 1752, 1779, 1895, 2336
MaeII	ACGT	2 cut(s)	801, 1212
MaeIII	GTNAC	4 cut(s)	108, 176, 503, 802
MalI	GATC	8 cut(s)	225, 270, 705, 996, 1203, 1316, 1528, 2064
MbiI	CCGCTC	1 cut(s)	697
MboI	GATC	8 cut(s)	223, 268, 703, 994, 1201, 1314, 1526, 2062
MboII	GAAGA	8 cut(s)	157, 270, 327, 847, 1303, 1558, 2138, 2141
MfeI	CAATTG	2 cut(s)	1530, 1767
MflI	RGATCY	2 cut(s)	1201, 2062
MhlI	GDGCHC	1 cut(s)	70
MlyI	GAGTC	3 cut(s)	1300, 1545, 2341
MmeI	TCCRAC	2 cut(s)	1639, 2127
Mph1103I	ATGCAT	3 cut(s)	436, 1037, 1820
MroXI	GAANNNNTTC	3 cut(s)	839, 1710, 1848
MseI	TTAA	8 cut(s)	131, 368, 1059, 1142, 1448, 1623, 1719, 2422
MslI	CAYNNNNRTG	3 cut(s)	1002, 1361, 1541
MspCI	CTTAAG	1 cut(s)	1141
MspI	CCGG	3 cut(s)	117, 140, 1924
MspR9I	CCNGG	5 cut(s)	102, 486, 496, 544, 570
MunI	CAATTG	2 cut(s)	1530, 1767
Mva1269I	GAATGC	3 cut(s)	1820, 2028, 2217
MvaI	CCWGG	5 cut(s)	102, 486, 496, 544, 570
NdeII	GATC	8 cut(s)	223, 268, 703, 994, 1201, 1314, 1526, 2062
NlaIII	CATG	9 cut(s)	796, 1158, 1190, 1690, 1802, 1942, 2199, 2388, 2396
NlaIV	GGNNCC	5 cut(s)	482, 493, 1420, 1757, 1948
NmuCI	GTSAC	2 cut(s)	108, 802
NsiI	ATGCAT	3 cut(s)	436, 1037, 1820
NspI	RCATGY	2 cut(s)	1690, 2199
PaeI	GCATGC	1 cut(s)	2199
PaeR7I	CTCGAG	1 cut(s)	171
PagI	TCATGA	2 cut(s)	1798, 2392
PctI	GAATGC	3 cut(s)	1820, 2028, 2217
PdmI	GAANNNNTTC	3 cut(s)	839, 1710, 1848
PfeI	GAWTC	8 cut(s)	4, 291, 761, 1090, 1668, 1706, 2342, 2381
PflMI	CCANNNNNTGG	1 cut(s)	569
PkrI	GCNGC	1 cut(s)	2372
PleI	GAGTC	3 cut(s)	1300, 1545, 2340
PpsI	GAGTC	3 cut(s)	1300, 1545, 2340
PshBI	ATTAAT	1 cut(s)	1623
Psp124BI	GAGCTC	1 cut(s)	70
Psp6I	CCWGG	5 cut(s)	100, 484, 494, 542, 568
PspFI	CCCAGC	1 cut(s)	562
PspGI	CCWGG	5 cut(s)	100, 484, 494, 542, 568
PspN4I	GGNNCC	5 cut(s)	482, 493, 1420, 1757, 1948
PspPI	GGNCC	2 cut(s)	322, 653
PspXI	VCTCGAGB	1 cut(s)	171
PstI	CTGCAG	1 cut(s)	769
PstNI	CAGNNNCTG	3 cut(s)	569, 773, 1274
PsuI	RGATCY	2 cut(s)	1201, 2062
RsaI	GTAC	9 cut(s)	209, 791, 1159, 1329, 1368, 1757, 2360, 2365, 2399
RsaNI	GTAC	9 cut(s)	208, 790, 1158, 1328, 1367, 1756, 2359, 2364, 2398
RseI	CAYNNNNRTG	3 cut(s)	1002, 1361, 1541
SacI	GAGCTC	1 cut(s)	70
SaqAI	TTAA	8 cut(s)	131, 368, 1059, 1142, 1448, 1623, 1719, 2422
SatI	GCNGC	1 cut(s)	2371
Sau3AI	GATC	8 cut(s)	223, 268, 703, 994, 1201, 1314, 1526, 2062
Sau96I	GGNCC	2 cut(s)	322, 653
ScaI	AGTACT	3 cut(s)	1329, 2365, 2399
SchI	GAGTC	3 cut(s)	1300, 1545, 2341
ScrFI	CCNGG	5 cut(s)	102, 486, 496, 544, 570
SduI	GDGCHC	1 cut(s)	70
SfaNI	GCATC	9 cut(s)	421, 443, 924, 1264, 1390, 1849, 2065, 2128, 2275
SfcI	CTRYAG	1 cut(s)	765
Sfr274I	CTCGAG	1 cut(s)	171
SinI	GGWCC	1 cut(s)	322
SlaI	CTCGAG	1 cut(s)	171
SmiMI	CAYNNNNRTG	3 cut(s)	1002, 1361, 1541
SmlI	CTYRAG	3 cut(s)	171, 584, 1141
SmoI	CTYRAG	3 cut(s)	171, 584, 1141
SphI	GCATGC	1 cut(s)	2199
SsiI	CCGC	4 cut(s)	381, 697, 1078, 2320
SspMI	CTAG	7 cut(s)	530, 912, 1703, 1752, 1779, 1895, 2336
SstI	GAGCTC	1 cut(s)	70
StyD4I	CCNGG	5 cut(s)	100, 484, 494, 542, 568
StyI	CCWWGG	2 cut(s)	656, 1422
TaaI	ACNGT	6 cut(s)	232, 639, 1118, 1559, 1727, 2363
TaiI	ACGT	2 cut(s)	804, 1215
TaqI	TCGA	3 cut(s)	172, 1384, 2316
TatI	WGTACW	4 cut(s)	1157, 1327, 2363, 2397
TfiI	GAWTC	8 cut(s)	4, 291, 761, 1090, 1668, 1706, 2342, 2381
Tru1I	TTAA	8 cut(s)	131, 368, 1059, 1142, 1448, 1623, 1719, 2422
Tru9I	TTAA	8 cut(s)	131, 368, 1059, 1142, 1448, 1623, 1719, 2422
TscAI	CASTG	5 cut(s)	811, 854, 1123, 1548, 1832
TseFI	GTSAC	2 cut(s)	108, 802
TseI	GCWGC	1 cut(s)	2370
Tsp45I	GTSAC	2 cut(s)	108, 802
TspDTI	ATGAA	6 cut(s)	37, 846, 975, 1787, 1955, 2373
TspRI	CASTG	5 cut(s)	811, 854, 1123, 1548, 1832
Van91I	CCANNNNNTGG	1 cut(s)	569
Vha464I	CTTAAG	1 cut(s)	1141
VpaK11BI	GGWCC	1 cut(s)	322
VspI	ATTAAT	1 cut(s)	1623
XagI	CCTNNNNNAGG	1 cut(s)	1799
XapI	RAATTY	5 cut(s)	149, 355, 853, 1263, 2292
XbaI	TCTAGA	1 cut(s)	1702
XceI	RCATGY	2 cut(s)	1690, 2199
XcmI	CCANNNNNNNNNTGG	2 cut(s)	474, 1766
XhoI	CTCGAG	1 cut(s)	171
XmnI	GAANNNNTTC	3 cut(s)	839, 1710, 1848
XspI	CTAG	7 cut(s)	530, 912, 1703, 1752, 1779, 1895, 2336
ZraI	GACGTC	1 cut(s)	802
ZrmI	AGTACT	3 cut(s)	1329, 2365, 2399
Zsp2I	ATGCAT	3 cut(s)	436, 1037, 1820

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.