RchiOBHm_Chr2g0127821

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000916
Physical Location & Seq
Forward (+)
42665748 .. 42670236
4489 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49966

Sequence Viewer

Length: 1593 bp
ATGGATATCAATAAAAGGAATAGATTCATTTCTAGTTATTTTCTGCTTCAGCTATGTATTGCCCTGAGAGCTTATATCACAATGGGGTCTGGCAGTCTGTCACCAGGCCAATTTCTTCTTGTACACCAGAAAATTACTTCTCCAAGAGGCATATTTGAGCTAGGTTTCTTCACACCAGGTAACTCTCAAAACTATTACATAGGCATCTGGTACAAAAATGTACCCAATCAAATAGTTGTTTGGGTGGCAAACAGAAACCAACCGATTATTGATCCAACTTCCTCATCATTTGTACTCCTTGAAAATGGAAACTTGACCTTAAATAGCCCCTCAATGGTGGCAATATGGTCAACTAATTCAACATCAAAGGGTAAAAATTCTACCATAGCCATGCTTCTTGACAATGGAAACTTTGTCATAAGAGATGCATTTGATTCGTCTGATGTTATATGGCAGAGTTTCGACCACCCAACAGATACTTGGCTGCCAGGTGCAAAGCTTGGTTATAATATGCTTACCAAGGAAAGCCTAATTCTTACTCCCTGGAGAAACCCAGAAAATCCAGTGCCTAGTCTAAATCTGCTGGGTTCTTGTATTTTGATTCTTTCTCATCTGGGTTTGGCTTGGTTTGTCCAGTCTTGCTGGTTTCGGGATATGGGTGCTTTTCAATATTCATGCTTTGAACTCAGAACACTTGTGTTTGGTGGGTTAGCCAGGCGAATGTCAACAAGCTTCATTTCTTGTTACATTGATATACATGGTGTTAATTTCAGTTGTTCCTGCTCTGAAGAGCACTCACCGCTTTCCTCTTGCTGCTCTAGATCTCAACCTACACCACTCTGGCCAGCAATAAATTCAAAGAAACGACGCCGTCGAGCGAGCTGTGCTCAGACAACTCAAACGAGATGGAGTCTCCAACGGTGTCCGTCCAATTTGTATCTTTGCGGTTGCTTGAAAGGATTTGAACCGAAAGTAGCTGAAGATTGGAAACTAGAAGACCACTCAGAAGGCTGTGTGAGAAATGCTCCTTTTAACTGCAGTGATGAAAGCAATAACACATTTATCATCATGCCTCATGTTCTTTATCCAGTAAATTTCGAGTCTTTCGCAGTTGCAAACCTAGACGAATGTAAATCAGCATGCTTAAGAAATTGCTCATGCACTGCTTTTGCTTATGATAGCCAGTGTCTGGTTTGGAAAGGAGATCTGTTCAATCTAAGGCAACTAACATCTGATGGAAAATTGAGCAAAGCTTTACATCTTCGAGTTGCAGCATTTGAGAAAAATCAAATGATAGACTCTACAGATAAGAAAAGGAGAGAAACTACGTGGATTGTTATTGTAGTACTTTCTAGCTTCTTTGGTTTGTTAAGCTTTATGATGGTATTCATCAGAAAGAAACAGTCATCAGAAGAGATGGAAACAGACGAGGATTCTTTGCTGCTATTCAAGTACAAAGATTTGAGAAGAGCAACACACAACTTCTCACAAAAAATTGGGGAAGGAGCTTTTGGTTCCGTTTTCAAGGTGACGTCGCTCTTGGGAAGCGCGCAATTTAACCCTGAAAATATCGTTAGTAGTATAAGCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_2 Pkinase S_locus_glycop

Protein Analysis

530

Amino Acids

59.81

Weight (kDa)

8.67

Isoelectric Point (pI)

54.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 79 - 183 2.3e-32 D-mannose binding lectin
PAN_2 PF08276 346 - 405 5.2e-13 PAN-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 507
AatII GACGTC 1 cut(s) 1535
AccB7I CCANNNNNTGG 1 cut(s) 1189
AccII CGCG 1 cut(s) 1550
AciI CCGC 2 cut(s) 800, 945
AclWI GGATC 1 cut(s) 266
AcoI YGGCCR 1 cut(s) 842
AcsI RAATTY 3 cut(s) 376, 853, 1093
AcuI CTGAAG 3 cut(s) 32, 807, 999
AcyI GRCGYC 2 cut(s) 868, 1532
AfaI GTAC 6 cut(s) 123, 212, 222, 294, 1347, 1454
AfiI CCNNNNNNNGG 2 cut(s) 334, 1189
AflII CTTAAG 1 cut(s) 1144
AjnI CCWGG 5 cut(s) 103, 175, 487, 542, 713
AjuI GAANNNNNNNTTGG 2 cut(s) 1494, 1526
Alw21I GWGCWC 2 cut(s) 795, 889
Alw26I GTCTC 1 cut(s) 917
AlwI GGATC 1 cut(s) 266
AlwNI CAGNNNCTG 1 cut(s) 1189
AoxI GGCC 2 cut(s) 106, 842
ApeKI GCWGC 4 cut(s) 484, 813, 1271, 1441
ApoI RAATTY 3 cut(s) 376, 853, 1093
Asp700I GAANNNNTTC 1 cut(s) 23
AspLEI GCGC 2 cut(s) 1550, 1552
AsuHPI GGTGA 3 cut(s) 93, 789, 1540
BalI TGGCCA 1 cut(s) 844
BbsI GAAGAC 1 cut(s) 1002
Bbv12I GWGCWC 2 cut(s) 795, 889
BbvI GCAGC 4 cut(s) 471, 800, 1283, 1428
BccI CCATC 4 cut(s) 900, 1229, 1375, 1411
BceAI ACGGC 1 cut(s) 855
BcgI CGANNNNNNTGC 2 cut(s) 417, 451
BciT130I CCWGG 5 cut(s) 105, 177, 489, 544, 715
BcoDI GTCTC 1 cut(s) 917
BfaI CTAG 7 cut(s) 33, 161, 570, 819, 992, 1121, 1353
BfmI CTRYAG 2 cut(s) 1036, 1302
BfrI CTTAAG 1 cut(s) 1144
BglII AGATCT 2 cut(s) 821, 1204
BisI GCNGC 4 cut(s) 485, 814, 1272, 1442
BlsI GCNGC 4 cut(s) 486, 815, 1273, 1443
BmcAI AGTACT 1 cut(s) 1347
Bme1390I CCNGG 5 cut(s) 105, 177, 489, 544, 715
BmiI GGNNCC 1 cut(s) 1516
BmrFI CCNGG 5 cut(s) 105, 177, 489, 544, 715
BmsI GCATC 2 cut(s) 213, 415
BpiI GAAGAC 1 cut(s) 1002
BplI GAGNNNNNCTC 4 cut(s) 871, 903, 1009, 1041
BpmI CTGGAG 1 cut(s) 565
BsaAI YACGTR 1 cut(s) 1329
BsaHI GRCGYC 2 cut(s) 868, 1532
BsaJI CCNNGG 2 cut(s) 519, 542
Bsc4I CCNNNNNNNGG 2 cut(s) 334, 1189
Bse1I ACTGG 4 cut(s) 563, 634, 1088, 1183
BseBI CCWGG 5 cut(s) 105, 177, 489, 544, 715
BseDI CCNNGG 2 cut(s) 519, 542
BseLI CCNNNNNNNGG 2 cut(s) 334, 1189
BseMII CTCAG 4 cut(s) 56, 700, 902, 1017
BseNI ACTGG 4 cut(s) 563, 634, 1088, 1183
BsePI GCGCGC 1 cut(s) 1548
BseXI GCAGC 4 cut(s) 471, 800, 1283, 1428
BseYI CCCAGC 1 cut(s) 583
Bsh1236I CGCG 1 cut(s) 1550
BshFI GGCC 2 cut(s) 108, 844
BsiHKAI GWGCWC 2 cut(s) 795, 889
BslI CCNNNNNNNGG 2 cut(s) 334, 1189
BsmAI GTCTC 1 cut(s) 917
BsnI GGCC 2 cut(s) 108, 844
Bsp1286I GDGCHC 2 cut(s) 795, 889
Bsp1407I TGTACA 1 cut(s) 121
Bsp143I GATC 3 cut(s) 271, 821, 1204
BspACI CCGC 2 cut(s) 800, 945
BspANI GGCC 2 cut(s) 108, 844
BspCNI CTCAG 4 cut(s) 57, 699, 901, 1016
BspFNI CGCG 1 cut(s) 1550
BspLI GGNNCC 1 cut(s) 1516
BspMAI CTGCAG 1 cut(s) 1040
BspPI GGATC 1 cut(s) 266
BspQI GCTCTTC 2 cut(s) 783, 1462
BspTI CTTAAG 1 cut(s) 1144
BsrGI TGTACA 1 cut(s) 121
BsrI ACTGG 4 cut(s) 563, 634, 1088, 1183
BssECI CCNNGG 2 cut(s) 519, 542
BssHII GCGCGC 1 cut(s) 1548
BssMI GATC 3 cut(s) 271, 821, 1204
BssNI GRCGYC 2 cut(s) 868, 1532
BssT1I CCWWGG 1 cut(s) 519
Bst2UI CCWGG 5 cut(s) 105, 177, 489, 544, 715
Bst4CI ACNGT 2 cut(s) 921, 1404
Bst6I CTCTTC 3 cut(s) 783, 1407, 1462
BstACI GRCGYC 2 cut(s) 868, 1532
BstAFI CTTAAG 1 cut(s) 1144
BstAUI TGTACA 1 cut(s) 121
BstBAI YACGTR 1 cut(s) 1329
BstC8I GCNNGC 4 cut(s) 846, 880, 1141, 1550
BstDEI CTNAG 5 cut(s) 65, 686, 888, 1003, 1217
BstFNI CGCG 1 cut(s) 1550
BstHHI GCGC 2 cut(s) 1550, 1552
BstKTI GATC 3 cut(s) 274, 824, 1207
BstMAI GTCTC 1 cut(s) 917
BstMBI GATC 3 cut(s) 271, 821, 1204
BstMWI GCNNNNNNNGC 3 cut(s) 68, 799, 884
BstNI CCWGG 5 cut(s) 105, 177, 489, 544, 715
BstNSI RCATGY 1 cut(s) 1143
BstSCI CCNGG 5 cut(s) 103, 175, 487, 542, 713
BstSFI CTRYAG 2 cut(s) 1036, 1302
BstUI CGCG 1 cut(s) 1550
BstV1I GCAGC 4 cut(s) 471, 800, 1283, 1428
BstV2I GAAGAC 1 cut(s) 1002
BstX2I RGATCY 2 cut(s) 821, 1204
BstYI RGATCY 2 cut(s) 821, 1204
BsuRI GGCC 2 cut(s) 108, 844
BtsI GCAGTG 2 cut(s) 1045, 1161
BtsIMutI CAGTG 4 cut(s) 570, 1045, 1161, 1190
Cac8I GCNNGC 4 cut(s) 846, 880, 1141, 1550
CaiI CAGNNNCTG 1 cut(s) 1189
CfoI GCGC 2 cut(s) 1550, 1552
CseI GACGC 1 cut(s) 876
CsiI ACCWGGT 1 cut(s) 175
Csp6I GTAC 6 cut(s) 122, 211, 221, 293, 1346, 1453
CviAII CATG 7 cut(s) 391, 675, 758, 1069, 1076, 1140, 1158
CviQI GTAC 6 cut(s) 122, 211, 221, 293, 1346, 1453
DdeI CTNAG 5 cut(s) 65, 686, 888, 1003, 1217
DpnI GATC 3 cut(s) 273, 823, 1206
DpnII GATC 3 cut(s) 271, 821, 1204
EaeI YGGCCR 1 cut(s) 842
Eam1104I CTCTTC 3 cut(s) 783, 1407, 1462
EarI CTCTTC 3 cut(s) 783, 1407, 1462
Eco130I CCWWGG 1 cut(s) 519
Eco32I GATATC 1 cut(s) 7
Eco57I CTGAAG 3 cut(s) 32, 807, 999
EcoRII CCWGG 5 cut(s) 103, 175, 487, 542, 713
EcoRV GATATC 1 cut(s) 7
EcoT14I CCWWGG 1 cut(s) 519
EcoT22I ATGCAT 1 cut(s) 430
ErhI CCWWGG 1 cut(s) 519
FaeI CATG 7 cut(s) 394, 678, 761, 1072, 1079, 1143, 1161
FatI CATG 7 cut(s) 390, 674, 757, 1068, 1075, 1139, 1157
Fnu4HI GCNGC 4 cut(s) 485, 814, 1272, 1442
Fsp4HI GCNGC 4 cut(s) 485, 814, 1272, 1442
FspBI CTAG 7 cut(s) 33, 161, 570, 819, 992, 1121, 1353
GlaI GCGC 2 cut(s) 1549, 1551
GluI GCNGC 4 cut(s) 485, 814, 1272, 1442
GsaI CCCAGC 1 cut(s) 587
GsuI CTGGAG 1 cut(s) 565
HaeIII GGCC 2 cut(s) 108, 844
HgaI GACGC 1 cut(s) 876
HhaI GCGC 2 cut(s) 1550, 1552
Hin1I GRCGYC 2 cut(s) 868, 1532
Hin1II CATG 7 cut(s) 394, 678, 761, 1072, 1079, 1143, 1161
Hin6I GCGC 2 cut(s) 1548, 1550
HinP1I GCGC 2 cut(s) 1548, 1550
HincII GTYRAC 2 cut(s) 351, 726
HindII GTYRAC 2 cut(s) 351, 726
HindIII AAGCTT 4 cut(s) 497, 730, 1251, 1372
HinfI GANTC 7 cut(s) 24, 434, 601, 910, 1100, 1298, 1433
HphI GGTGA 3 cut(s) 93, 789, 1540
Hpy166II GTNNAC 3 cut(s) 124, 351, 726
Hpy188I TCNGA 8 cut(s) 442, 689, 787, 891, 1006, 1234, 1394, 1411
Hpy188III TCNNGA 3 cut(s) 398, 650, 819
Hpy8I GTNNAC 3 cut(s) 124, 351, 726
Hpy99I CGWCG 3 cut(s) 870, 876, 1537
HpyAV CCTTC 2 cut(s) 1001, 1496
HpyCH4III ACNGT 2 cut(s) 921, 1404
HpyCH4IV ACGT 2 cut(s) 1328, 1532
HpyCH4V TGCA 6 cut(s) 428, 494, 1038, 1115, 1161, 1271
HpyF10VI GCNNNNNNNGC 3 cut(s) 68, 799, 884
HpyF3I CTNAG 5 cut(s) 65, 686, 888, 1003, 1217
HpySE526I ACGT 2 cut(s) 1328, 1532
Hsp92I GRCGYC 2 cut(s) 868, 1532
Hsp92II CATG 7 cut(s) 394, 678, 761, 1072, 1079, 1143, 1161
HspAI GCGC 2 cut(s) 1548, 1550
Kzo9I GATC 3 cut(s) 271, 821, 1204
LguI GCTCTTC 2 cut(s) 783, 1462
LmnI GCTCC 2 cut(s) 1030, 1505
Lsp1109I GCAGC 4 cut(s) 471, 800, 1283, 1428
LweI GCATC 2 cut(s) 213, 415
MabI ACCWGGT 1 cut(s) 175
MaeI CTAG 7 cut(s) 33, 161, 570, 819, 992, 1121, 1353
MaeII ACGT 2 cut(s) 1328, 1532
MaeIII GTNAC 4 cut(s) 99, 179, 743, 1528
MalI GATC 3 cut(s) 273, 823, 1206
MboI GATC 3 cut(s) 271, 821, 1204
MboII GAAGA 8 cut(s) 107, 160, 800, 992, 1007, 1253, 1424, 1479
MflI RGATCY 2 cut(s) 821, 1204
MhlI GDGCHC 2 cut(s) 795, 889
MlsI TGGCCA 1 cut(s) 844
MluNI TGGCCA 1 cut(s) 844
MlyI GAGTC 3 cut(s) 919, 1109, 1292
MmeI TCCRAC 2 cut(s) 299, 940
MnlI CCTC 6 cut(s) 140, 292, 340, 817, 1083, 1423
Mox20I TGGCCA 1 cut(s) 844
Mph1103I ATGCAT 1 cut(s) 430
MroXI GAANNNNTTC 1 cut(s) 23
MscI TGGCCA 1 cut(s) 844
MseI TTAA 6 cut(s) 320, 765, 1032, 1145, 1370, 1557
MslI CAYNNNNRTG 1 cut(s) 389
Msp20I TGGCCA 1 cut(s) 844
MspCI CTTAAG 1 cut(s) 1144
MspR9I CCNGG 5 cut(s) 105, 177, 489, 544, 715
MvaI CCWGG 5 cut(s) 105, 177, 489, 544, 715
MvnI CGCG 1 cut(s) 1550
MwoI GCNNNNNNNGC 3 cut(s) 68, 799, 884
NdeII GATC 3 cut(s) 271, 821, 1204
NlaIII CATG 7 cut(s) 394, 678, 761, 1072, 1079, 1143, 1161
NlaIV GGNNCC 1 cut(s) 1516
NmuCI GTSAC 2 cut(s) 99, 1528
NsiI ATGCAT 1 cut(s) 430
NspI RCATGY 1 cut(s) 1143
PaeI GCATGC 1 cut(s) 1143
PauI GCGCGC 1 cut(s) 1548
PciSI GCTCTTC 2 cut(s) 783, 1462
PcsI WCGNNNNNNNCGW 1 cut(s) 871
PdmI GAANNNNTTC 1 cut(s) 23
PfeI GAWTC 4 cut(s) 24, 434, 601, 1433
PflFI GACNNNGTC 1 cut(s) 870
PflMI CCANNNNNTGG 1 cut(s) 1189
PkrI GCNGC 4 cut(s) 486, 815, 1273, 1443
PleI GAGTC 3 cut(s) 918, 1108, 1292
PpsI GAGTC 3 cut(s) 918, 1108, 1292
Ppu21I YACGTR 1 cut(s) 1329
PsiI TTATAA 1 cut(s) 507
Psp6I CCWGG 5 cut(s) 103, 175, 487, 542, 713
PspFI CCCAGC 1 cut(s) 583
PspGI CCWGG 5 cut(s) 103, 175, 487, 542, 713
PspN4I GGNNCC 1 cut(s) 1516
PstI CTGCAG 1 cut(s) 1040
PstNI CAGNNNCTG 1 cut(s) 1189
PsuI RGATCY 2 cut(s) 821, 1204
PsyI GACNNNGTC 1 cut(s) 870
PteI GCGCGC 1 cut(s) 1548
RsaI GTAC 6 cut(s) 123, 212, 222, 294, 1347, 1454
RsaNI GTAC 6 cut(s) 122, 211, 221, 293, 1346, 1453
RseI CAYNNNNRTG 1 cut(s) 389
SapI GCTCTTC 2 cut(s) 783, 1462
SaqAI TTAA 6 cut(s) 320, 765, 1032, 1145, 1370, 1557
SatI GCNGC 4 cut(s) 485, 814, 1272, 1442
Sau3AI GATC 3 cut(s) 271, 821, 1204
ScaI AGTACT 1 cut(s) 1347
SchI GAGTC 3 cut(s) 919, 1109, 1292
ScrFI CCNGG 5 cut(s) 105, 177, 489, 544, 715
SduI GDGCHC 2 cut(s) 795, 889
SexAI ACCWGGT 1 cut(s) 175
SfaNI GCATC 2 cut(s) 213, 415
SfcI CTRYAG 2 cut(s) 1036, 1302
SmiMI CAYNNNNRTG 1 cut(s) 389
SmlI CTYRAG 1 cut(s) 1144
SmoI CTYRAG 1 cut(s) 1144
SphI GCATGC 1 cut(s) 1143
SsiI CCGC 2 cut(s) 800, 945
SspI AATATT 1 cut(s) 671
SspMI CTAG 7 cut(s) 33, 161, 570, 819, 992, 1121, 1353
StyD4I CCNGG 5 cut(s) 103, 175, 487, 542, 713
StyI CCWWGG 1 cut(s) 519
TaaI ACNGT 2 cut(s) 921, 1404
TaiI ACGT 2 cut(s) 1331, 1535
TaqI TCGA 4 cut(s) 462, 874, 1098, 1264
TatI WGTACW 4 cut(s) 121, 292, 1345, 1452
TfiI GAWTC 4 cut(s) 24, 434, 601, 1433
Tru1I TTAA 6 cut(s) 320, 765, 1032, 1145, 1370, 1557
Tru9I TTAA 6 cut(s) 320, 765, 1032, 1145, 1370, 1557
TscAI CASTG 4 cut(s) 570, 1045, 1168, 1190
TseFI GTSAC 2 cut(s) 99, 1528
TseI GCWGC 4 cut(s) 484, 813, 1271, 1441
Tsp45I GTSAC 2 cut(s) 99, 1528
TspDTI ATGAA 5 cut(s) 16, 663, 724, 1059, 1378
TspGWI ACGGA 2 cut(s) 915, 1507
TspRI CASTG 4 cut(s) 570, 1045, 1168, 1190
Tth111I GACNNNGTC 1 cut(s) 870
Van91I CCANNNNNTGG 1 cut(s) 1189
Vha464I CTTAAG 1 cut(s) 1144
XapI RAATTY 3 cut(s) 376, 853, 1093
XbaI TCTAGA 1 cut(s) 818
XceI RCATGY 1 cut(s) 1143
XcmI CCANNNNNNNNNTGG 1 cut(s) 477
XmnI GAANNNNTTC 1 cut(s) 23
XspI CTAG 7 cut(s) 33, 161, 570, 819, 992, 1121, 1353
ZraI GACGTC 1 cut(s) 1533
ZrmI AGTACT 1 cut(s) 1347
Zsp2I ATGCAT 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.