Rw2G026230

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000916
Physical Location & Seq
Reverse (-)
40452591 .. 40455011
2421 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G026230.1

Sequence Viewer

Length: 2277 bp
ATGAAAACTAGTATCAGAAACCACTTGATTTCCCAGTCTCTGCTACTCTTGTTATCTTACACTACTTGTACTCCCAAAGCTATTGAGATTCTGTTACCAGGCCAATCTCTCAATGGGGACCAGCGGATCACCTCTCCTGGTGGCATTTTTGAACTTGGTTTTTTCACACCAGGTAACTCTCAAAACCATTACATAGGCATCTGGTACAAAAATCTACCCAATCAAACAGTTGTTTGGGTGGCAAACAGAGACCAACCTGTTTCTGATCCTATTTCTTCAACATTTCTACTCCATAAAAATGGAAACTTGACCTTAAACAGCCCCTCAACAGTGGCGATATGGTCAACAAATTCAACGTCGCAGGTTAGAAATTCTACTATAGCCATGCTTCTTGACGATGGCAACTTTGTCATAAGAAATGCATCCGCAGTACTATGGCAGAGTTTCGATCATCCAACCAATACTTGGCTGCCAGGTGCAAGGCTTGGATATAACAAGCTTACCAAGCAAATCCTAAGTATTACCCCCTGGAGAAATCCGCAATATCCAGCACCTGGTCCCTTTTCTTTTGAGCTAGAACAAACAAGTCTTCGGCTATTATGGAATAGTTCGGAAACTTACTGGACCAGCGGTAATTGGACAGGTAAAACTTTCAGCCATCTTCAAGACATCGAAGAGAAGCATGTTCCCTCAATTTTCTCATTTGTTTTAAATGAGACTGGAAGCTACCTTAGTTATGCAGTTCGTGCTTCTGCCTACCCTGATCCCTTTATTAGGAATCATACCTTTATTAGGTACATGATAGAAACCACTGGTCAAATGAAGTTCTTTGTCATGGAGAACGGCTCCCTGCAATGGAATTTGCTATGGATGGATCCAATGGAACGATGTGATGTTTATGCTTCCTGTGGTGCATTTAGTATATGCAACCCACGTAATCTTCCTCGGTGTGGCTGTCTAAAAGGATTCGAACCCAAATTCCATAAAGACTGGGAATTAGAAGATCACTCAAATGGCTGTGGGAGGAAAACTTCTTTTCATTGCAGTGGTGAGGGGAATTCTGCATTCCTGGTGATGGAGAATGTTACTTACCCGAAGAGTCCTGACTCCTTGGTAGTTGATAACATTGACAAATGTAGATTAACTTGCTTGAGAAATTGCTCATGCACTGCTTTTGCTTATGATAATCAGTGTCTGATTTGGGGGGGGGGTGAACTGTTTAATGTAAAGCAACTATCATCTGATGGAATGATTGGAAAAGTTTGGTATCTACGAGTAGCAGCGTCTGAGAAGGAAATAATAGATTCTACAGCTGGGAATACAAAGAGAAAGACTACTTGGATTGTGATTGGAGTACTAGCAGGAATCTTTACTCTCTTTAGCATTGTGTTGGTAGTTGTCAGACGGAGACAGTCTGTTCGAGCATTGAAGTCTTTTGAACATTCTCTAGTGCTATTCAACTACAGAGATTTTAAAAAAAGCAACAGAAAACTTCTCAGAGAAACTTGGAGAAGGAGCTTTTGGTTCTGTTTTCAAGGGGATATTAAGACTTTAGTTTATGACTACATGCCAAATGGCTCTCTACAATCTGTTCTAGTTCAAAACCATCCACTAATCTTAGATTGGAATGCAAGATATCATATTGCCATAGGCATTGCAAGAGGATTGGCTTACCTCCATGAGGACTGTAGAGATTGTATAATACACTGTGACATTAAGCCAGAGAACATTTTGTTGGATGAAGAATATAATCCAAAAGTTGGAGATTTTGGTCTTGCAAAACTCATTGGCAGACACCATAGTCGAGTTCTGACAAGCATGAGAGGAACTGTTGGATATCTAGCACCAGAATGGTTTACAGGTGAAGCCATCACACCAAAAGCGGATGTCTTCAGCTATGGAAAGTTGCTGTTTGAGGTTATATCTGGAAGGAGAAACAGTGAAGGGTTAGATGATGGATTAGAAAACTACACTCCTAACCAGGTTGCAAATGCAGTAACTAAAGGACAAGATGTTCTTACCTTGGTTGATTACAGGTTGGGAGGTAATGCTGACAAGGATGAGCTCATTAGAGCATGCAAAGTTGCGTGTTGGTGTATTCAAGATGTGGAGAAGGATAGGCCAACAATGAGGCAGGTTGTTCAAATTCTCGAAGGAGCTTTAGATGTTGGTATACCCCCGATCCCACAGTTTCTCCAGTACCTTTCCGGAAGTTTAACGGAAGCAATCAATTACCGAGAACCTACTTCAAGTTCAGAAATCATTTTCCTAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_2 Pkinase S_locus_glycop

Protein Analysis

758

Amino Acids

85.72

Weight (kDa)

7.76

Isoelectric Point (pI)

40.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 76 - 178 3e-30 D-mannose binding lectin
S_locus_glycop PF00954 208 - 327 3.8e-22 S-locus glycoprotein domain
PAN_2 PF08276 348 - 408 5.5e-12 PAN-like domain
PK_Tyr_Ser-Thr PF07714 510 - 717 2.1e-25 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 517 - 715 2.8e-32 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1800
Acc36I ACCTGC 2 cut(s) 352, 2125
AccB7I CCANNNNNTGG 3 cut(s) 465, 554, 1760
AccI GTMKAC 1 cut(s) 2173
AccIII TCCGGA 1 cut(s) 2207
AciI CCGC 5 cut(s) 124, 426, 539, 630, 1883
AclWI GGATC 6 cut(s) 134, 260, 758, 869, 882, 2176
AcsI RAATTY 6 cut(s) 349, 370, 859, 977, 1057, 2145
AcuI CTGAAG 1 cut(s) 1876
AfaI GTAC 6 cut(s) 70, 206, 432, 797, 1356, 2201
AfiI CCNNNNNNNGG 9 cut(s) 465, 554, 774, 792, 855, 950, 1075, 1681, 1760
AhlI ACTAGT 1 cut(s) 8
AjnI CCWGG 8 cut(s) 97, 136, 169, 472, 527, 553, 1068, 1980
AjuI GAANNNNNNNTTGG 6 cut(s) 1321, 1353, 1504, 1536, 1718, 1750
AloI GAACNNNNNNTCC 4 cut(s) 963, 995, 1998, 2030
Alw21I GWGCWC 1 cut(s) 2067
Alw26I GTCTC 4 cut(s) 42, 243, 710, 1402
AlwI GGATC 6 cut(s) 134, 260, 758, 869, 882, 2176
AlwNI CAGNNNCTG 4 cut(s) 40, 554, 1195, 1286
Aor13HI TCCGGA 1 cut(s) 2207
AoxI GGCC 2 cut(s) 100, 2120
ApeKI GCWGC 2 cut(s) 469, 1280
ApoI RAATTY 6 cut(s) 349, 370, 859, 977, 1057, 2145
ArsI GACNNNNNNTTYG 4 cut(s) 341, 373, 1401, 1433
AspS9I GGNCC 3 cut(s) 118, 557, 624
AsuHPI GGTGA 5 cut(s) 121, 1061, 1084, 1223, 1874
AsuII TTCGAA 1 cut(s) 969
AvaII GGWCC 3 cut(s) 118, 557, 624
BamHI GGATCC 1 cut(s) 874
BanII GRGCYC 1 cut(s) 2067
BbsI GAAGAC 2 cut(s) 581, 1882
Bbv12I GWGCWC 1 cut(s) 2067
BbvI GCAGC 2 cut(s) 456, 1292
BccI CCATC 8 cut(s) 392, 666, 865, 1069, 1238, 1614, 1877, 1949
BceAI ACGGC 1 cut(s) 859
BciT130I CCWGG 8 cut(s) 99, 138, 171, 474, 529, 555, 1070, 1982
BcoDI GTCTC 4 cut(s) 42, 243, 710, 1402
BcuI ACTAGT 1 cut(s) 8
BfaI CTAG 6 cut(s) 9, 575, 1358, 1448, 1595, 1841
BfmI CTRYAG 4 cut(s) 378, 1308, 1462, 1687
BfuAI ACCTGC 2 cut(s) 352, 2125
BisI GCNGC 2 cut(s) 470, 1281
BlsI GCNGC 2 cut(s) 471, 1282
BmcAI AGTACT 2 cut(s) 432, 1356
Bme1390I CCNGG 8 cut(s) 99, 138, 171, 474, 529, 555, 1070, 1982
Bme18I GGWCC 3 cut(s) 118, 557, 624
BmgT120I GGNCC 3 cut(s) 118, 557, 624
BmiI GGNNCC 4 cut(s) 119, 559, 847, 876
BmrFI CCNGG 8 cut(s) 99, 138, 171, 474, 529, 555, 1070, 1982
BmrI ACTGGG 2 cut(s) 28, 1000
BmsI GCATC 2 cut(s) 207, 431
BmuI ACTGGG 2 cut(s) 28, 1000
BpiI GAAGAC 2 cut(s) 581, 1882
BplI GAGNNNNNCTC 2 cut(s) 830, 862
BpmI CTGGAG 2 cut(s) 550, 2180
Bpu10I CCTNAGC 1 cut(s) 2270
Bpu14I TTCGAA 1 cut(s) 969
BpuEI CTTGAG 1 cut(s) 1171
BsaAI YACGTR 1 cut(s) 935
BsaI GGTCTC 1 cut(s) 243
BsaJI CCNNGG 4 cut(s) 527, 944, 1110, 2022
BsaWI WCCGGW 1 cut(s) 2207
BsaXI ACNNNNNCTCC 6 cut(s) 55, 85, 1924, 1954, 2178, 2208
Bsc4I CCNNNNNNNGG 9 cut(s) 465, 554, 774, 792, 855, 950, 1075, 1681, 1760
Bse1I ACTGG 6 cut(s) 34, 626, 724, 817, 995, 2197
Bse3DI GCAATG 3 cut(s) 860, 1039, 1653
BseAI TCCGGA 1 cut(s) 2207
BseBI CCWGG 8 cut(s) 99, 138, 171, 474, 529, 555, 1070, 1982
BseDI CCNNGG 4 cut(s) 527, 944, 1110, 2022
BseGI GGATG 7 cut(s) 422, 451, 876, 1606, 1744, 1891, 2065
BseLI CCNNNNNNNGG 9 cut(s) 465, 554, 774, 792, 855, 950, 1075, 1681, 1760
BseMI GCAATG 3 cut(s) 860, 1039, 1653
BseMII CTCAG 2 cut(s) 1278, 1510
BseNI ACTGG 6 cut(s) 34, 626, 724, 817, 995, 2197
BseXI GCAGC 2 cut(s) 456, 1292
BseYI CCCAGC 1 cut(s) 1313
BshFI GGCC 2 cut(s) 102, 2122
BsiHKAI GWGCWC 1 cut(s) 2067
BsiSI CCGG 1 cut(s) 2208
BslFI GGGAC 2 cut(s) 131, 543
BslI CCNNNNNNNGG 9 cut(s) 465, 554, 774, 792, 855, 950, 1075, 1681, 1760
BsmAI GTCTC 4 cut(s) 42, 243, 710, 1402
BsmFI GGGAC 2 cut(s) 131, 543
BsmI GAATGC 2 cut(s) 1064, 1633
BsnI GGCC 2 cut(s) 102, 2122
Bso31I GGTCTC 1 cut(s) 243
Bsp119I TTCGAA 1 cut(s) 969
Bsp1286I GDGCHC 1 cut(s) 2067
Bsp13I TCCGGA 1 cut(s) 2207
Bsp143I GATC 7 cut(s) 126, 265, 448, 763, 874, 1003, 2181
BspACI CCGC 5 cut(s) 124, 426, 539, 630, 1883
BspANI GGCC 2 cut(s) 102, 2122
BspCNI CTCAG 2 cut(s) 1279, 1509
BspEI TCCGGA 1 cut(s) 2207
BspLI GGNNCC 4 cut(s) 119, 559, 847, 876
BspMI ACCTGC 2 cut(s) 352, 2125
BspPI GGATC 6 cut(s) 134, 260, 758, 869, 882, 2176
BspT104I TTCGAA 1 cut(s) 969
BspTNI GGTCTC 1 cut(s) 243
BsrDI GCAATG 3 cut(s) 860, 1039, 1653
BsrI ACTGG 6 cut(s) 34, 626, 724, 817, 995, 2197
BssECI CCNNGG 4 cut(s) 527, 944, 1110, 2022
BssMI GATC 7 cut(s) 126, 265, 448, 763, 874, 1003, 2181
BssNAI GTATAC 1 cut(s) 2174
BssT1I CCWWGG 2 cut(s) 1110, 2022
Bst1107I GTATAC 1 cut(s) 2174
Bst2UI CCWGG 8 cut(s) 99, 138, 171, 474, 529, 555, 1070, 1982
Bst4CI ACNGT 9 cut(s) 229, 331, 1218, 1413, 1688, 1709, 1831, 1940, 2190
Bst6I CTCTTC 2 cut(s) 669, 1091
BstAPI GCANNNNNTGC 1 cut(s) 746
BstBAI YACGTR 1 cut(s) 935
BstBI TTCGAA 1 cut(s) 969
BstC8I GCNNGC 1 cut(s) 2077
BstDEI CTNAG 6 cut(s) 515, 731, 1287, 1496, 1618, 2270
BstENI CCTNNNNNAGG 3 cut(s) 772, 790, 1679
BstF5I GGATG 7 cut(s) 422, 451, 876, 1606, 1744, 1891, 2065
BstKTI GATC 7 cut(s) 129, 268, 451, 766, 877, 1006, 2184
BstMAI GTCTC 4 cut(s) 42, 243, 710, 1402
BstMBI GATC 7 cut(s) 126, 265, 448, 763, 874, 1003, 2181
BstMWI GCNNNNNNNGC 2 cut(s) 505, 746
BstNI CCWGG 8 cut(s) 99, 138, 171, 474, 529, 555, 1070, 1982
BstNSI RCATGY 3 cut(s) 686, 1570, 2079
BstSCI CCNGG 8 cut(s) 97, 136, 169, 472, 527, 553, 1068, 1980
BstSFI CTRYAG 4 cut(s) 378, 1308, 1462, 1687
BstV1I GCAGC 2 cut(s) 456, 1292
BstV2I GAAGAC 2 cut(s) 581, 1882
BstX2I RGATCY 1 cut(s) 874
BstXI CCANNNNNNTGG 1 cut(s) 299
BstYI RGATCY 1 cut(s) 874
BstZ17I GTATAC 1 cut(s) 2174
BsuRI GGCC 2 cut(s) 102, 2122
BtsCI GGATG 7 cut(s) 422, 451, 876, 1606, 1744, 1891, 2065
BtsI GCAGTG 2 cut(s) 1051, 1167
BtsIMutI CAGTG 7 cut(s) 336, 810, 1051, 1167, 1196, 1705, 1945
BveI ACCTGC 2 cut(s) 352, 2125
Cac8I GCNNGC 1 cut(s) 2077
CaiI CAGNNNCTG 4 cut(s) 40, 554, 1195, 1286
Cfr13I GGNCC 3 cut(s) 118, 557, 624
CseI GACGC 1 cut(s) 1272
CsiI ACCWGGT 3 cut(s) 169, 553, 1980
Csp6I GTAC 6 cut(s) 69, 205, 431, 796, 1355, 2200
CviAII CATG 9 cut(s) 385, 683, 799, 835, 1164, 1567, 1679, 1819, 2076
CviQI GTAC 6 cut(s) 69, 205, 431, 796, 1355, 2200
DdeI CTNAG 6 cut(s) 515, 731, 1287, 1496, 1618, 2270
DpnI GATC 7 cut(s) 128, 267, 450, 765, 876, 1005, 2183
DpnII GATC 7 cut(s) 126, 265, 448, 763, 874, 1003, 2181
DraI TTTAAA 2 cut(s) 711, 1474
DrdI GACNNNNNNGTC 1 cut(s) 1800
DseDI GACNNNNNNGTC 1 cut(s) 1800
Eam1104I CTCTTC 2 cut(s) 669, 1091
EarI CTCTTC 2 cut(s) 669, 1091
Ecl136II GAGCTC 1 cut(s) 2065
Eco130I CCWWGG 2 cut(s) 1110, 2022
Eco24I GRGCYC 1 cut(s) 2067
Eco31I GGTCTC 1 cut(s) 243
Eco32I GATATC 2 cut(s) 1637, 1838
Eco47I GGWCC 3 cut(s) 118, 557, 624
Eco53kI GAGCTC 1 cut(s) 2065
Eco57I CTGAAG 1 cut(s) 1876
EcoICRI GAGCTC 1 cut(s) 2065
EcoNI CCTNNNNNAGG 3 cut(s) 772, 790, 1679
EcoRI GAATTC 1 cut(s) 1057
EcoRII CCWGG 8 cut(s) 97, 136, 169, 472, 527, 553, 1068, 1980
EcoRV GATATC 2 cut(s) 1637, 1838
EcoT14I CCWWGG 2 cut(s) 1110, 2022
EcoT22I ATGCAT 1 cut(s) 424
EcoT38I GRGCYC 1 cut(s) 2067
ErhI CCWWGG 2 cut(s) 1110, 2022
FaeI CATG 9 cut(s) 388, 686, 802, 838, 1167, 1570, 1682, 1822, 2079
FalI AAGNNNNNCTT 2 cut(s) 2001, 2033
FaqI GGGAC 2 cut(s) 131, 543
FatI CATG 9 cut(s) 384, 682, 798, 834, 1163, 1566, 1678, 1818, 2075
FblI GTMKAC 1 cut(s) 2173
Fnu4HI GCNGC 2 cut(s) 470, 1281
FokI GGATG 7 cut(s) 409, 438, 883, 1593, 1751, 1898, 2072
FriOI GRGCYC 1 cut(s) 2067
Fsp4HI GCNGC 2 cut(s) 470, 1281
FspBI CTAG 6 cut(s) 9, 575, 1358, 1448, 1595, 1841
GluI GCNGC 2 cut(s) 470, 1281
GsaI CCCAGC 1 cut(s) 1317
GsuI CTGGAG 2 cut(s) 550, 2180
HaeIII GGCC 2 cut(s) 102, 2122
HapII CCGG 1 cut(s) 2208
HgaI GACGC 1 cut(s) 1272
Hin1II CATG 9 cut(s) 388, 686, 802, 838, 1167, 1570, 1682, 1822, 2079
HincII GTYRAC 1 cut(s) 345
HindII GTYRAC 1 cut(s) 345
HindIII AAGCTT 1 cut(s) 497
HinfI GANTC 7 cut(s) 88, 778, 966, 1099, 1106, 1304, 1365
HpaII CCGG 1 cut(s) 2208
HphI GGTGA 5 cut(s) 121, 1061, 1084, 1223, 1874
Hpy166II GTNNAC 4 cut(s) 345, 1214, 1857, 2174
Hpy188III TCNNGA 7 cut(s) 392, 665, 1103, 1926, 2102, 2150, 2208
Hpy8I GTNNAC 4 cut(s) 345, 1214, 1857, 2174
Hpy99I CGWCG 1 cut(s) 361
HpyAV CCTTC 6 cut(s) 1285, 1506, 1923, 1937, 2107, 2147
HpyCH4III ACNGT 9 cut(s) 229, 331, 1218, 1413, 1688, 1709, 1831, 1940, 2190
HpyCH4IV ACGT 2 cut(s) 356, 934
HpyF10VI GCNNNNNNNGC 2 cut(s) 505, 746
HpyF3I CTNAG 6 cut(s) 515, 731, 1287, 1496, 1618, 2270
HpySE526I ACGT 2 cut(s) 356, 934
Hsp92II CATG 9 cut(s) 388, 686, 802, 838, 1167, 1570, 1682, 1822, 2079
Kpn2I TCCGGA 1 cut(s) 2207
Kzo9I GATC 7 cut(s) 126, 265, 448, 763, 874, 1003, 2181
LmnI GCTCC 3 cut(s) 851, 1515, 2156
Lsp1109I GCAGC 2 cut(s) 456, 1292
LweI GCATC 2 cut(s) 207, 431
MabI ACCWGGT 3 cut(s) 169, 553, 1980
MaeI CTAG 6 cut(s) 9, 575, 1358, 1448, 1595, 1841
MaeII ACGT 2 cut(s) 356, 934
MaeIII GTNAC 5 cut(s) 93, 173, 1084, 1709, 1996
MalI GATC 7 cut(s) 128, 267, 450, 765, 876, 1005, 2183
MboI GATC 7 cut(s) 126, 265, 448, 763, 874, 1003, 2181
MboII GAAGA 9 cut(s) 267, 581, 653, 686, 932, 1013, 1108, 1754, 1882
MflI RGATCY 1 cut(s) 874
MhlI GDGCHC 1 cut(s) 2067
MlyI GAGTC 2 cut(s) 1100, 1108
MmeI TCCRAC 4 cut(s) 479, 1716, 1741, 1813
Mph1103I ATGCAT 1 cut(s) 424
MroI TCCGGA 1 cut(s) 2207
MseI TTAA 8 cut(s) 314, 710, 1142, 1221, 1473, 1545, 1716, 2216
MslI CAYNNNNRTG 4 cut(s) 297, 1011, 1044, 1849
MspA1I CMGCKG 3 cut(s) 124, 630, 1313
MspI CCGG 1 cut(s) 2208
MspR9I CCNGG 8 cut(s) 99, 138, 171, 474, 529, 555, 1070, 1982
Mva1269I GAATGC 2 cut(s) 1064, 1633
MvaI CCWGG 8 cut(s) 99, 138, 171, 474, 529, 555, 1070, 1982
MwoI GCNNNNNNNGC 2 cut(s) 505, 746
NdeII GATC 7 cut(s) 126, 265, 448, 763, 874, 1003, 2181
NlaIII CATG 9 cut(s) 388, 686, 802, 838, 1167, 1570, 1682, 1822, 2079
NlaIV GGNNCC 4 cut(s) 119, 559, 847, 876
NmuCI GTSAC 1 cut(s) 1709
NsiI ATGCAT 1 cut(s) 424
NspI RCATGY 3 cut(s) 686, 1570, 2079
NspV TTCGAA 1 cut(s) 969
PaeI GCATGC 1 cut(s) 2079
PctI GAATGC 2 cut(s) 1064, 1633
PfeI GAWTC 5 cut(s) 88, 778, 966, 1304, 1365
PflMI CCANNNNNTGG 3 cut(s) 465, 554, 1760
PkrI GCNGC 2 cut(s) 471, 1282
PleI GAGTC 2 cut(s) 1100, 1107
PpsI GAGTC 2 cut(s) 1100, 1107
Ppu21I YACGTR 1 cut(s) 935
Psp124BI GAGCTC 1 cut(s) 2067
Psp6I CCWGG 8 cut(s) 97, 136, 169, 472, 527, 553, 1068, 1980
PspFI CCCAGC 1 cut(s) 1313
PspGI CCWGG 8 cut(s) 97, 136, 169, 472, 527, 553, 1068, 1980
PspN4I GGNNCC 4 cut(s) 119, 559, 847, 876
PspPI GGNCC 3 cut(s) 118, 557, 624
PstNI CAGNNNCTG 4 cut(s) 40, 554, 1195, 1286
PsuI RGATCY 1 cut(s) 874
PvuII CAGCTG 1 cut(s) 1313
RsaI GTAC 6 cut(s) 70, 206, 432, 797, 1356, 2201
RsaNI GTAC 6 cut(s) 69, 205, 431, 796, 1355, 2200
RseI CAYNNNNRTG 4 cut(s) 297, 1011, 1044, 1849
SacI GAGCTC 1 cut(s) 2067
SaqAI TTAA 8 cut(s) 314, 710, 1142, 1221, 1473, 1545, 1716, 2216
SatI GCNGC 2 cut(s) 470, 1281
Sau3AI GATC 7 cut(s) 126, 265, 448, 763, 874, 1003, 2181
Sau96I GGNCC 3 cut(s) 118, 557, 624
ScaI AGTACT 2 cut(s) 432, 1356
SchI GAGTC 2 cut(s) 1100, 1108
ScrFI CCNGG 8 cut(s) 99, 138, 171, 474, 529, 555, 1070, 1982
SduI GDGCHC 1 cut(s) 2067
SexAI ACCWGGT 3 cut(s) 169, 553, 1980
SfaNI GCATC 2 cut(s) 207, 431
SfcI CTRYAG 4 cut(s) 378, 1308, 1462, 1687
SfuI TTCGAA 1 cut(s) 969
SinI GGWCC 3 cut(s) 118, 557, 624
SmiMI CAYNNNNRTG 4 cut(s) 297, 1011, 1044, 1849
SmlI CTYRAG 1 cut(s) 1150
SmoI CTYRAG 1 cut(s) 1150
SpeI ACTAGT 1 cut(s) 8
SphI GCATGC 1 cut(s) 2079
SsiI CCGC 5 cut(s) 124, 426, 539, 630, 1883
SspMI CTAG 6 cut(s) 9, 575, 1358, 1448, 1595, 1841
SstI GAGCTC 1 cut(s) 2067
StyD4I CCNGG 8 cut(s) 97, 136, 169, 472, 527, 553, 1068, 1980
StyI CCWWGG 2 cut(s) 1110, 2022
TaaI ACNGT 9 cut(s) 229, 331, 1218, 1413, 1688, 1709, 1831, 1940, 2190
TaiI ACGT 2 cut(s) 359, 937
TaqI TCGA 6 cut(s) 447, 672, 969, 1420, 1804, 2151
TatI WGTACW 3 cut(s) 68, 430, 1354
TfiI GAWTC 5 cut(s) 88, 778, 966, 1304, 1365
Tru1I TTAA 8 cut(s) 314, 710, 1142, 1221, 1473, 1545, 1716, 2216
Tru9I TTAA 8 cut(s) 314, 710, 1142, 1221, 1473, 1545, 1716, 2216
TscAI CASTG 7 cut(s) 336, 817, 1051, 1174, 1196, 1712, 1945
TseFI GTSAC 1 cut(s) 1709
TseI GCWGC 2 cut(s) 469, 1280
Tsp45I GTSAC 1 cut(s) 1709
TspDTI ATGAA 4 cut(s) 17, 836, 1028, 1755
TspGWI ACGGA 2 cut(s) 1420, 2234
TspRI CASTG 7 cut(s) 336, 817, 1051, 1174, 1196, 1712, 1945
Van91I CCANNNNNTGG 3 cut(s) 465, 554, 1760
VpaK11BI GGWCC 3 cut(s) 118, 557, 624
XagI CCTNNNNNAGG 3 cut(s) 772, 790, 1679
XapI RAATTY 6 cut(s) 349, 370, 859, 977, 1057, 2145
XceI RCATGY 3 cut(s) 686, 1570, 2079
XcmI CCANNNNNNNNNTGG 2 cut(s) 110, 462
XmiI GTMKAC 1 cut(s) 2173
XspI CTAG 6 cut(s) 9, 575, 1358, 1448, 1595, 1841
ZrmI AGTACT 2 cut(s) 432, 1356
Zsp2I ATGCAT 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.