Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0000927

Physical Location & Seq

Genomic Coordinates Forward (+)

2236039 .. 2240004

Gene Structure 3966 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1031500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1257 bp

ATGAGCAGTCGAGCAGTGGAAGACGACGCACTGGCCAAAGCCGCAAGAGCTGCCGAAGCTCTTTACCTCCTCCGAGACACCTACTTTCCGGCCGACCCGGATGACAAAATCTCCAGATTAAACGCCGATTCCGATCTTGCCCTCAACCTCCTCGATTCCATTCCTCTTGAGCAAAGAAAACATCCGGCTCAACGTGCGAAATATGAATATCTGAGAGGAAAGATACTGGATGTTTTACCGAATTATAGAAAAGAAGCAGAGGATCATCTCTCAAAAGCTGTTAAGTTGAATCCATCTCTTGCAGATGCTTGGCTATGTTTAGGTAACTGCATTTGGAAGAAGGGAGATCTATCTTCAGCCAAGAACTGCTTTACTCTTGCCCTAAGCAAGGGTCCAAACAGGCAGATACTTTGTCAACTTTCGATGCTTGAAAGAAAAATGGCTCAAGGTACCGACAATGAGGCAGAACTTGTTGATGAAAGCATTCAACATGCCAAGGAAGCCGTAACTCTTGATGTCAAGGATGGGAATTCATGGTACAATCTTGGAAATGCATGTCTTACAAGTTTTTTTGTGACTGGAGCTTGGGACCATTCCAAACTTCTGCAATCTTTGAAGGCATACCAAAATGCTGAAAAGGATGAAAGAATGCAGTCCAATCCAGACCTATATTTTAACTGTGCCACTGTAAACAGATATCTGGAAAACTATGAAAGGGCCCTTTCTGGATTTGAAGCTGCTGCTTTAAAGGATCCTGGTCTCAATGCTACAGAGGAGGTTCAAAAGATTGTTAGCCTTCTTGACAGGCTGGATAATCTGTTAAGGGGGCATGCTAGATCTAAACGACTTCTGTCTTTAGCATCATCTCCGGCTGGTGTAAACTTGAACTCCTCATATCAAAGAGCTACTGTAGATATTCTATCAGAGGGTCTGAACAAATCAGTAGCACTAGTGGGAAAAGTGCTATTCTTTATTAAGCATGACAACGTTGCTCCACTGTACTACTTGGCATGCGATTCAAATCAAATATGCTTTGTTGTGTCAGTTTATGGCATTCAATATGATTCGATCAAAGAAGGAGATCAGCTAACAATACTGGAACCTTTTCATCGGCGTTTCAAGTTTTCTTGGAAAGAAAAGTTCTACGACTTCAAATCATTTCGTGTGGATTTCATAGAACAGTTGCTTGTGAACGGAAAGGCCCTTACTCCTCACCAAGCTGTCCGTACCTCAATTTATGCACAGCACAAACCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0008150 GO:0009966 GO:0010646 GO:0023051 GO:0031974 GO:0031981 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0048583 GO:0050789 GO:0050794 GO:0065007 GO:0070013 GO:1901796 GO:1902531

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TTC5_OB

Protein Analysis

419

Amino Acids

47.1

Weight (kDa)

7.1

Isoelectric Point (pI)

31.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TTC5_OB	PF16669	298 - 410	3.1e-37	Tetratricopeptide repeat protein 5 OB fold domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000927)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g26200 FvH4_4g26200 FvH4_4g26200
malus_domestica	MD16G1031500.v1.1
prunus_persica	Prupe.1G321400_v2.0.a1 Prupe.1G321400_v2.0.a1 Prupe.1G321400_v2.0.a1 Prupe.1G321400_v2.0.a1
pyrus_communis	pycom16g02770
rosa_chinensis	RchiOBHm_Chr2g0138811 RchiOBHm_Chr3g0493761 RchiOBHm_Chr4g0433781 RchiOBHm_Chr7g0235151
rosa_laevigata	RLG00000006731 RLG00000010288 RLG00000022530 RLG00000033694 RLG00000034398
rosa_multiflora	Rmu_sc0000580.1_g000036 Rmu_sc0001535.1_g000003 Rmu_sc0002212.1_g000008 Rmu_sc0004245.1_g000010 Rmu_sc0033149.1_g000001
rosa_roxburghii	Rroxscaffold_1G00006660 Rroxscaffold_2G00147750 Rroxscaffold_3G00241970 Rroxscaffold_5G00375210 Rroxscaffold_6G00389040 Rroxscaffold_6G00389720
rosa_rugosa	Rorug01G0124100 Rorug04G0271300 Rorug04G0271400 Rorug04G0271500 Rorug05G0199400 Rorug06G0215900
rosa_samantha	Rh1BG293100 Rh1CG238700 Rh1CG309600 Rh1DG324600 Rh2AG337400 Rh2BG392100 Rh2BG454700 Rh2BG454800 Rh2CG324700 Rh2DG464800 Rh3AG312700 Rh3DG293500 Rh4AG049900 Rh4AG326100 Rh4BG333700 Rh4CG349200 Rh4DG028200 Rh4DG330700 Rh5AG399500 Rh5AG400700 Rh5CG248100 Rh5DG415200 Rh6AG088100 Rh6BG202700 Rh6CG077100 Rh7CG463900
rosa_wichuraiana	Rw4G028230 Rw4G028280 Rw7G040770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	449
AccB1I	GGYRCC	1 cut(s)	449
AciI	CCGC	1 cut(s)	42
AclI	AACGTT	1 cut(s)	987
AclWI	GGATC	3 cut(s)	270, 746, 759
AcoI	YGGCCR	2 cut(s)	33, 90
AcsI	RAATTY	1 cut(s)	529
AcuI	CTGAAG	1 cut(s)	339
AfaI	GTAC	4 cut(s)	451, 539, 1001, 1228
AfiI	CCNNNNNNNGG	1 cut(s)	388
AhlI	ACTAGT	1 cut(s)	949
AjnI	CCWGG	1 cut(s)	754
AluBI	AGCT	8 cut(s)	50, 59, 278, 584, 737, 905, 1087, 1220
AluI	AGCT	8 cut(s)	50, 59, 278, 584, 737, 905, 1087, 1220
Alw26I	GTCTC	2 cut(s)	69, 764
AlwI	GGATC	3 cut(s)	270, 746, 759
AoxI	GGCC	4 cut(s)	33, 90, 717, 1200
ApaI	GGGCCC	1 cut(s)	721
ApeKI	GCWGC	3 cut(s)	50, 737, 740
ApoI	RAATTY	1 cut(s)	529
Asp700I	GAANNNNTTC	2 cut(s)	483, 1104
Asp718I	GGTACC	1 cut(s)	449
AspS9I	GGNCC	5 cut(s)	392, 589, 717, 718, 1201
AsuC2I	CCSGG	1 cut(s)	98
AsuHPI	GGTGA	1 cut(s)	1205
AvaII	GGWCC	2 cut(s)	392, 589
BaeGI	GKGCMC	1 cut(s)	721
BalI	TGGCCA	1 cut(s)	35
BamHI	GGATCC	1 cut(s)	751
BanI	GGYRCC	1 cut(s)	449
BanII	GRGCYC	1 cut(s)	721
BbsI	GAAGAC	1 cut(s)	27
BbvI	GCAGC	3 cut(s)	37, 724, 727
BccI	CCATC	2 cut(s)	301, 518
BceAI	ACGGC	1 cut(s)	488
BciT130I	CCWGG	1 cut(s)	756
BcnI	CCSGG	1 cut(s)	98
BcoDI	GTCTC	2 cut(s)	69, 764
BcuI	ACTAGT	1 cut(s)	949
BfaI	CTAG	2 cut(s)	834, 950
BfmI	CTRYAG	2 cut(s)	768, 909
BglII	AGATCT	2 cut(s)	346, 836
BisI	GCNGC	4 cut(s)	42, 51, 738, 741
BlsI	GCNGC	4 cut(s)	43, 52, 739, 742
Bme1390I	CCNGG	2 cut(s)	98, 756
Bme18I	GGWCC	2 cut(s)	392, 589
BmgT120I	GGNCC	5 cut(s)	392, 589, 717, 718, 1201
BmiI	GGNNCC	6 cut(s)	393, 451, 590, 719, 753, 1101
BmrFI	CCNGG	2 cut(s)	98, 756
BmsI	GCATC	3 cut(s)	295, 414, 869
BoxI	GACNNNNGTC	1 cut(s)	850
BpiI	GAAGAC	1 cut(s)	27
BpmI	CTGGAG	2 cut(s)	97, 600
Bpu10I	CCTNAGC	1 cut(s)	383
BpuEI	CTTGAG	2 cut(s)	188, 429
BpuMI	CCSGG	1 cut(s)	98
BsaBI	GATNNNNATC	2 cut(s)	132, 1020
BsaI	GGTCTC	1 cut(s)	764
BsaJI	CCNNGG	1 cut(s)	495
BsaXI	ACNNNNNCTCC	4 cut(s)	95, 125, 872, 902
Bsc4I	CCNNNNNNNGG	1 cut(s)	388
Bse1I	ACTGG	4 cut(s)	36, 231, 583, 1101
Bse8I	GATNNNNATC	2 cut(s)	132, 1020
BseBI	CCWGG	1 cut(s)	756
BseDI	CCNNGG	1 cut(s)	495
BseGI	GGATG	5 cut(s)	106, 181, 235, 529, 646
BseJI	GATNNNNATC	2 cut(s)	132, 1020
BseLI	CCNNNNNNNGG	1 cut(s)	388
BseMII	CTCAG	1 cut(s)	203
BseNI	ACTGG	4 cut(s)	36, 231, 583, 1101
BseRI	GAGGAG	5 cut(s)	59, 140, 788, 880, 1200
BseSI	GKGCMC	1 cut(s)	721
BseX3I	CGGCCG	1 cut(s)	90
BseXI	GCAGC	3 cut(s)	37, 724, 727
Bsh1285I	CGRYCG	1 cut(s)	93
BshFI	GGCC	4 cut(s)	35, 92, 719, 1202
BshNI	GGYRCC	1 cut(s)	449
BsiEI	CGRYCG	1 cut(s)	93
BsiSI	CCGG	4 cut(s)	89, 98, 185, 869
BslFI	GGGAC	1 cut(s)	602
BslI	CCNNNNNNNGG	1 cut(s)	388
BsmAI	GTCTC	2 cut(s)	69, 764
BsmFI	GGGAC	1 cut(s)	602
BsmI	GAATGC	3 cut(s)	483, 654, 1053
BsnI	GGCC	4 cut(s)	35, 92, 719, 1202
Bso31I	GGTCTC	1 cut(s)	764
Bsp120I	GGGCCC	1 cut(s)	717
Bsp1286I	GDGCHC	1 cut(s)	721
Bsp143I	GATC	7 cut(s)	133, 262, 346, 751, 836, 1068, 1081
BspACI	CCGC	1 cut(s)	42
BspANI	GGCC	4 cut(s)	35, 92, 719, 1202
BspCNI	CTCAG	1 cut(s)	204
BspLI	GGNNCC	6 cut(s)	393, 451, 590, 719, 753, 1101
BspPI	GGATC	3 cut(s)	270, 746, 759
BspT107I	GGYRCC	1 cut(s)	449
BspTNI	GGTCTC	1 cut(s)	764
BsrI	ACTGG	4 cut(s)	36, 231, 583, 1101
BssECI	CCNNGG	1 cut(s)	495
BssMI	GATC	7 cut(s)	133, 262, 346, 751, 836, 1068, 1081
BssT1I	CCWWGG	1 cut(s)	495
Bst2UI	CCWGG	1 cut(s)	756
Bst4CI	ACNGT	5 cut(s)	680, 688, 910, 999, 1182
BstAPI	GCANNNNNTGC	1 cut(s)	50
BstC8I	GCNNGC	2 cut(s)	831, 1012
BstDEI	CTNAG	2 cut(s)	212, 383
BstENI	CCTNNNNNAGG	1 cut(s)	386
BstF5I	GGATG	5 cut(s)	106, 181, 235, 529, 646
BstKTI	GATC	7 cut(s)	136, 265, 349, 754, 839, 1071, 1084
BstMAI	GTCTC	2 cut(s)	69, 764
BstMBI	GATC	7 cut(s)	133, 262, 346, 751, 836, 1068, 1081
BstMCI	CGRYCG	1 cut(s)	93
BstMWI	GCNNNNNNNGC	6 cut(s)	41, 47, 50, 56, 194, 500
BstNI	CCWGG	1 cut(s)	756
BstNSI	RCATGY	4 cut(s)	494, 558, 833, 1014
BstPAI	GACNNNNGTC	1 cut(s)	850
BstSCI	CCNGG	2 cut(s)	96, 754
BstSFI	CTRYAG	2 cut(s)	768, 909
BstSLI	GKGCMC	1 cut(s)	721
BstV1I	GCAGC	3 cut(s)	37, 724, 727
BstV2I	GAAGAC	1 cut(s)	27
BstX2I	RGATCY	3 cut(s)	346, 751, 836
BstYI	RGATCY	3 cut(s)	346, 751, 836
BstZI	CGGCCG	1 cut(s)	90
BsuRI	GGCC	4 cut(s)	35, 92, 719, 1202
BtsCI	GGATG	5 cut(s)	106, 181, 235, 529, 646
BtsI	GCAGTG	1 cut(s)	21
BtsIMutI	CAGTG	4 cut(s)	21, 29, 684, 995
Cac8I	GCNNGC	2 cut(s)	831, 1012
Cfr13I	GGNCC	5 cut(s)	392, 589, 717, 718, 1201
CseI	GACGC	1 cut(s)	35
Csp6I	GTAC	4 cut(s)	450, 538, 1000, 1227
CviAII	CATG	7 cut(s)	491, 534, 555, 830, 980, 1011, 1254
CviQI	GTAC	4 cut(s)	450, 538, 1000, 1227
DdeI	CTNAG	2 cut(s)	212, 383
DpnI	GATC	7 cut(s)	135, 264, 348, 753, 838, 1070, 1083
DpnII	GATC	7 cut(s)	133, 262, 346, 751, 836, 1068, 1081
DraI	TTTAAA	1 cut(s)	747
EaeI	YGGCCR	2 cut(s)	33, 90
EagI	CGGCCG	1 cut(s)	90
EclXI	CGGCCG	1 cut(s)	90
Eco130I	CCWWGG	1 cut(s)	495
Eco24I	GRGCYC	1 cut(s)	721
Eco31I	GGTCTC	1 cut(s)	764
Eco32I	GATATC	1 cut(s)	698
Eco47I	GGWCC	2 cut(s)	392, 589
Eco52I	CGGCCG	1 cut(s)	90
Eco57I	CTGAAG	1 cut(s)	339
EcoNI	CCTNNNNNAGG	1 cut(s)	386
EcoO109I	RGGNCCY	3 cut(s)	717, 718, 1201
EcoRI	GAATTC	1 cut(s)	529
EcoRII	CCWGG	1 cut(s)	754
EcoRV	GATATC	1 cut(s)	698
EcoT14I	CCWWGG	1 cut(s)	495
EcoT22I	ATGCAT	1 cut(s)	556
EcoT38I	GRGCYC	1 cut(s)	721
ErhI	CCWWGG	1 cut(s)	495
FaeI	CATG	7 cut(s)	494, 537, 558, 833, 983, 1014, 1257
FalI	AAGNNNNNCTT	2 cut(s)	353, 385
FaqI	GGGAC	1 cut(s)	602
FatI	CATG	7 cut(s)	490, 533, 554, 829, 979, 1010, 1253
Fnu4HI	GCNGC	4 cut(s)	42, 51, 738, 741
FokI	GGATG	5 cut(s)	113, 168, 242, 536, 653
FriOI	GRGCYC	1 cut(s)	721
Fsp4HI	GCNGC	4 cut(s)	42, 51, 738, 741
FspBI	CTAG	2 cut(s)	834, 950
GluI	GCNGC	4 cut(s)	42, 51, 738, 741
GsuI	CTGGAG	2 cut(s)	97, 600
HaeIII	GGCC	4 cut(s)	35, 92, 719, 1202
HapII	CCGG	4 cut(s)	89, 98, 185, 869
HgaI	GACGC	1 cut(s)	35
Hin1II	CATG	7 cut(s)	494, 537, 558, 833, 983, 1014, 1257
HincII	GTYRAC	1 cut(s)	416
HindII	GTYRAC	1 cut(s)	416
HinfI	GANTC	5 cut(s)	128, 155, 289, 1016, 1064
HpaII	CCGG	4 cut(s)	89, 98, 185, 869
HphI	GGTGA	1 cut(s)	1205
Hpy166II	GTNNAC	4 cut(s)	416, 691, 880, 1192
Hpy188I	TCNGA	5 cut(s)	74, 133, 213, 925, 933
Hpy188III	TCNNGA	7 cut(s)	114, 167, 512, 662, 701, 726, 800
Hpy8I	GTNNAC	4 cut(s)	416, 691, 880, 1192
Hpy99I	CGWCG	1 cut(s)	29
HpyAV	CCTTC	4 cut(s)	334, 610, 806, 1070
HpyCH4III	ACNGT	5 cut(s)	680, 688, 910, 999, 1182
HpyCH4IV	ACGT	2 cut(s)	193, 987
HpyCH4V	TGCA	6 cut(s)	302, 330, 554, 607, 652, 1241
HpyF10VI	GCNNNNNNNGC	6 cut(s)	41, 47, 50, 56, 194, 500
HpyF3I	CTNAG	2 cut(s)	212, 383
HpySE526I	ACGT	2 cut(s)	193, 987
Hsp92II	CATG	7 cut(s)	494, 537, 558, 833, 983, 1014, 1257
KpnI	GGTACC	1 cut(s)	453
Kzo9I	GATC	7 cut(s)	133, 262, 346, 751, 836, 1068, 1081
LmnI	GCTCC	2 cut(s)	581, 997
Lsp1109I	GCAGC	3 cut(s)	37, 724, 727
LweI	GCATC	3 cut(s)	295, 414, 869
MaeI	CTAG	2 cut(s)	834, 950
MaeII	ACGT	2 cut(s)	193, 987
MaeIII	GTNAC	3 cut(s)	323, 505, 574
MalI	GATC	7 cut(s)	135, 264, 348, 753, 838, 1070, 1083
MboI	GATC	7 cut(s)	133, 262, 346, 751, 836, 1068, 1081
MboII	GAAGA	3 cut(s)	32, 345, 349
MflI	RGATCY	3 cut(s)	346, 751, 836
MhlI	GDGCHC	1 cut(s)	721
MlsI	TGGCCA	1 cut(s)	35
MluCI	AATT	3 cut(s)	241, 529, 1233
MluNI	TGGCCA	1 cut(s)	35
Mox20I	TGGCCA	1 cut(s)	35
Mph1103I	ATGCAT	1 cut(s)	556
MroXI	GAANNNNTTC	2 cut(s)	483, 1104
MscI	TGGCCA	1 cut(s)	35
MseI	TTAA	6 cut(s)	119, 282, 675, 746, 821, 975
Msp20I	TGGCCA	1 cut(s)	35
MspI	CCGG	4 cut(s)	89, 98, 185, 869
MspR9I	CCNGG	2 cut(s)	98, 756
Mva1269I	GAATGC	3 cut(s)	483, 654, 1053
MvaI	CCWGG	1 cut(s)	756
MwoI	GCNNNNNNNGC	6 cut(s)	41, 47, 50, 56, 194, 500
NciI	CCSGG	1 cut(s)	98
NdeII	GATC	7 cut(s)	133, 262, 346, 751, 836, 1068, 1081
NlaIII	CATG	7 cut(s)	494, 537, 558, 833, 983, 1014, 1257
NlaIV	GGNNCC	6 cut(s)	393, 451, 590, 719, 753, 1101
NmuCI	GTSAC	1 cut(s)	574
NsiI	ATGCAT	1 cut(s)	556
NspI	RCATGY	4 cut(s)	494, 558, 833, 1014
PaeI	GCATGC	2 cut(s)	833, 1014
PcsI	WCGNNNNNNNCGW	1 cut(s)	129
PctI	GAATGC	3 cut(s)	483, 654, 1053
PdmI	GAANNNNTTC	2 cut(s)	483, 1104
PfeI	GAWTC	5 cut(s)	128, 155, 289, 1016, 1064
PkrI	GCNGC	4 cut(s)	43, 52, 739, 742
PshAI	GACNNNNGTC	1 cut(s)	850
Psp1406I	AACGTT	1 cut(s)	987
Psp6I	CCWGG	1 cut(s)	754
PspGI	CCWGG	1 cut(s)	754
PspN4I	GGNNCC	6 cut(s)	393, 451, 590, 719, 753, 1101
PspOMI	GGGCCC	1 cut(s)	717
PspPI	GGNCC	5 cut(s)	392, 589, 717, 718, 1201
PsuI	RGATCY	3 cut(s)	346, 751, 836
RsaI	GTAC	4 cut(s)	451, 539, 1001, 1228
RsaNI	GTAC	4 cut(s)	450, 538, 1000, 1227
SaqAI	TTAA	6 cut(s)	119, 282, 675, 746, 821, 975
SatI	GCNGC	4 cut(s)	42, 51, 738, 741
Sau3AI	GATC	7 cut(s)	133, 262, 346, 751, 836, 1068, 1081
Sau96I	GGNCC	5 cut(s)	392, 589, 717, 718, 1201
ScrFI	CCNGG	2 cut(s)	98, 756
SduI	GDGCHC	1 cut(s)	721
SfaNI	GCATC	3 cut(s)	295, 414, 869
SfcI	CTRYAG	2 cut(s)	768, 909
SinI	GGWCC	2 cut(s)	392, 589
SmlI	CTYRAG	2 cut(s)	167, 444
SmoI	CTYRAG	2 cut(s)	167, 444
SpeI	ACTAGT	1 cut(s)	949
SphI	GCATGC	2 cut(s)	833, 1014
Sse9I	AATT	3 cut(s)	241, 529, 1233
SsiI	CCGC	1 cut(s)	42
SspMI	CTAG	2 cut(s)	834, 950
StyD4I	CCNGG	2 cut(s)	96, 754
StyI	CCWWGG	1 cut(s)	495
TaaI	ACNGT	5 cut(s)	680, 688, 910, 999, 1182
TaiI	ACGT	2 cut(s)	196, 990
TaqI	TCGA	4 cut(s)	10, 153, 422, 1067
TasI	AATT	3 cut(s)	241, 529, 1233
TatI	WGTACW	1 cut(s)	999
TauI	GCSGC	1 cut(s)	44
TfiI	GAWTC	5 cut(s)	128, 155, 289, 1016, 1064
Tru1I	TTAA	6 cut(s)	119, 282, 675, 746, 821, 975
Tru9I	TTAA	6 cut(s)	119, 282, 675, 746, 821, 975
TscAI	CASTG	4 cut(s)	21, 36, 691, 1002
TseFI	GTSAC	1 cut(s)	574
TseI	GCWGC	3 cut(s)	50, 737, 740
Tsp45I	GTSAC	1 cut(s)	574
TspDTI	ATGAA	7 cut(s)	219, 492, 522, 657, 726, 1097, 1162
TspGWI	ACGGA	2 cut(s)	1209, 1214
TspRI	CASTG	4 cut(s)	21, 36, 691, 1002
VpaK11BI	GGWCC	2 cut(s)	392, 589
XagI	CCTNNNNNAGG	1 cut(s)	386
XapI	RAATTY	1 cut(s)	529
XceI	RCATGY	4 cut(s)	494, 558, 833, 1014
XmnI	GAANNNNTTC	2 cut(s)	483, 1104
XspI	CTAG	2 cut(s)	834, 950
Zsp2I	ATGCAT	1 cut(s)	556

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.