Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0000927

Physical Location & Seq

Genomic Coordinates Reverse (-)

10001624 .. 10005482

Gene Structure 3859 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000006731

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1257 bp

ATGAGCACTCCAGCAGTGGAAAACGACGCACTGGAGAAAGCCGGTAGTGCCACCGAAGCTCTTTACTTAACTCGAGATACCTACTTTCCGGCGAACCCCGACGACAAAATCGCCAGACTAAAATCAGAATCCGATCTCGCTCTCAACCTCCTCGATTCCATTCCTCCCGAGCAAAGGAAGCTTCTAACGCAGCGTGCAAGGTACGAATATCTGAGAGGGAAGATATTGGATGTATTGCCGGAGTATAAAAGAGAGGCTGAGGATCATCTCTCGAAAGCGGTTAAGTTGAATCCATCTCTTGCGGATGCGTGGCTGTGCTTAGGTAACTGCATTTGGAAGAAGGGAGATCTGTCTTCGGCCAAGAACTGCTTGACTCTTGCACTAAGCAAAGGTCCAAACAAACAGATACTATGTCAACTCTCAATGCTCGAAAGGAAAATGGCCCAAGGTGCCGATAATCAGGCAGAACTTGTTGATGAGAGCATAAAACATGCCAAGGAAGCTATAACTTTGGATGTCAAGGATGGGAATTCATGGTACAATCTTGGAAATGCATGTCTTACAAGTTTTTTTGTGACCGGAGCCTGGGACCACGCCAAACTTCTGCAGTCTCTAAAGGCATACCAAAATGCTGAAAAAGATGAAAGGATGAAATCTAATCCAGACCTGTACTTTAACTGTGCTACTGTAAACAAATATCTAGAAAACTATGAAATGGCCCTTACTGGATTTGAAGCTGCTGCTTTAAAGGATCCTGGTCTCAATGCTACCGAAGAGGTTCAAAAGATTGTTAACCTTCTTGACAAGCTAGATAATTTGTTAAGGGGACATGCCAGATCTAAACGACTTGCATCTTTAGCATCATCTCTGGCTGTGGTAAATTTGAATTCGTCATATAAAAGGGCTACTGTAAATCTTTTATCAGAGGGTCTGAACAAAGCAGTAGCATTAGTTGGGAAAGTGCTATTCTTCATTAAGCATGAAAATGTTGCTCCACTGTACTATTTGGCATGCGATCCAGATCAAGTATGCTTTGTTCTGTCAGTCTATGGCATTCAATATGATTCAATCAAAGAAGGAGACCTGATAACACTAGTGGATCCCTTATACCGGCAGGTCGAATTTTCTTGGAAAGCAAAGTTTTACCAGTTCAAATCATTTCGTGTGGATTTCAAAGAACAATTGCTTGTGAATGGAAAGGCTCTGACTCCTCACCAAGCAGTCCGCACCTCAATTCATGCACAGCATAAACCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0008150 GO:0009966 GO:0010646 GO:0023051 GO:0031974 GO:0031981 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0048583 GO:0050789 GO:0050794 GO:0065007 GO:0070013 GO:1901796 GO:1902531

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TTC5_OB

Protein Analysis

419

Amino Acids

46.83

Weight (kDa)

7.97

Isoelectric Point (pI)

26.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TTC5_OB	PF16669	298 - 410	1.6e-36	Tetratricopeptide repeat protein 5 OB fold domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000927)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g26200 FvH4_4g26200 FvH4_4g26200
malus_domestica	MD16G1031500.v1.1
prunus_persica	Prupe.1G321400_v2.0.a1 Prupe.1G321400_v2.0.a1 Prupe.1G321400_v2.0.a1 Prupe.1G321400_v2.0.a1
pyrus_communis	pycom16g02770
rosa_chinensis	RchiOBHm_Chr2g0138811 RchiOBHm_Chr3g0493761 RchiOBHm_Chr4g0433781 RchiOBHm_Chr7g0235151
rosa_laevigata	RLG00000006731 RLG00000010288 RLG00000022530 RLG00000033694 RLG00000034398
rosa_multiflora	Rmu_sc0000580.1_g000036 Rmu_sc0001535.1_g000003 Rmu_sc0002212.1_g000008 Rmu_sc0004245.1_g000010 Rmu_sc0033149.1_g000001
rosa_roxburghii	Rroxscaffold_1G00006660 Rroxscaffold_2G00147750 Rroxscaffold_3G00241970 Rroxscaffold_5G00375210 Rroxscaffold_6G00389040 Rroxscaffold_6G00389720
rosa_rugosa	Rorug01G0124100 Rorug04G0271300 Rorug04G0271400 Rorug04G0271500 Rorug05G0199400 Rorug06G0215900
rosa_samantha	Rh1BG293100 Rh1CG238700 Rh1CG309600 Rh1DG324600 Rh2AG337400 Rh2BG392100 Rh2BG454700 Rh2BG454800 Rh2CG324700 Rh2DG464800 Rh3AG312700 Rh3DG293500 Rh4AG049900 Rh4AG326100 Rh4BG333700 Rh4CG349200 Rh4DG028200 Rh4DG330700 Rh5AG399500 Rh5AG400700 Rh5CG248100 Rh5DG415200 Rh6AG088100 Rh6BG202700 Rh6CG077100 Rh7CG463900
rosa_wichuraiana	Rw4G028230 Rw4G028280 Rw7G040770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1105
AccB1I	GGYRCC	1 cut(s)	449
AciI	CCGC	3 cut(s)	278, 302, 1225
AclWI	GGATC	6 cut(s)	270, 746, 759, 1010, 1094, 1107
AcoI	YGGCCR	1 cut(s)	357
AcsI	RAATTY	4 cut(s)	529, 880, 886, 1121
AfaI	GTAC	4 cut(s)	203, 539, 671, 1001
AfiI	CCNNNNNNNGG	3 cut(s)	174, 585, 1110
AgsI	TTSAA	8 cut(s)	289, 734, 782, 886, 1058, 1068, 1153, 1174
AhlI	ACTAGT	1 cut(s)	1093
AjnI	CCWGG	2 cut(s)	584, 754
AluBI	AGCT	5 cut(s)	59, 181, 503, 737, 808
AluI	AGCT	5 cut(s)	59, 181, 503, 737, 808
Alw21I	GWGCWC	1 cut(s)	8
Alw26I	GTCTC	3 cut(s)	615, 764, 1074
AlwI	GGATC	6 cut(s)	270, 746, 759, 1010, 1094, 1107
Ama87I	CYCGRG	2 cut(s)	72, 167
AoxI	GGCC	3 cut(s)	357, 441, 717
ApeKI	GCWGC	3 cut(s)	190, 737, 740
ApoI	RAATTY	4 cut(s)	529, 880, 886, 1121
Asp700I	GAANNNNTTC	1 cut(s)	777
AspS9I	GGNCC	4 cut(s)	392, 442, 589, 718
AsuHPI	GGTGA	1 cut(s)	1205
AvaI	CYCGRG	2 cut(s)	72, 167
AvaII	GGWCC	2 cut(s)	392, 589
BamHI	GGATCC	2 cut(s)	751, 1099
BanI	GGYRCC	1 cut(s)	449
BbsI	GAAGAC	1 cut(s)	345
Bbv12I	GWGCWC	1 cut(s)	8
BbvCI	CCTCAGC	1 cut(s)	258
BbvI	GCAGC	3 cut(s)	202, 724, 727
BccI	CCATC	2 cut(s)	301, 518
BciT130I	CCWGG	2 cut(s)	586, 756
BcoDI	GTCTC	3 cut(s)	615, 764, 1074
BcuI	ACTAGT	1 cut(s)	1093
BfaI	CTAG	3 cut(s)	701, 809, 1094
BfmI	CTRYAG	1 cut(s)	605
BfuAI	ACCTGC	1 cut(s)	1105
BglII	AGATCT	2 cut(s)	346, 836
BisI	GCNGC	3 cut(s)	191, 738, 741
BlsI	GCNGC	3 cut(s)	192, 739, 742
Bme1390I	CCNGG	2 cut(s)	586, 756
Bme18I	GGWCC	2 cut(s)	392, 589
BmeT110I	CYCGRG	2 cut(s)	72, 167
BmgT120I	GGNCC	4 cut(s)	392, 442, 589, 718
BmiI	GGNNCC	5 cut(s)	451, 583, 590, 753, 1101
BmrFI	CCNGG	2 cut(s)	586, 756
BmsI	GCATC	3 cut(s)	295, 860, 869
BpiI	GAAGAC	1 cut(s)	345
BpmI	CTGGAG	1 cut(s)	53
Bpu10I	CCTNAGC	2 cut(s)	258, 319
BsaBI	GATNNNNATC	1 cut(s)	1020
BsaI	GGTCTC	2 cut(s)	764, 1074
BsaJI	CCNNGG	3 cut(s)	445, 495, 585
BsaWI	WCCGGW	1 cut(s)	578
Bsc4I	CCNNNNNNNGG	3 cut(s)	174, 585, 1110
Bse118I	RCCGGY	2 cut(s)	41, 1110
Bse1I	ACTGG	3 cut(s)	36, 730, 1147
Bse8I	GATNNNNATC	1 cut(s)	1020
BseBI	CCWGG	2 cut(s)	586, 756
BseDI	CCNNGG	3 cut(s)	445, 495, 585
BseGI	GGATG	5 cut(s)	235, 310, 520, 529, 654
BseJI	GATNNNNATC	1 cut(s)	1020
BseLI	CCNNNNNNNGG	3 cut(s)	174, 585, 1110
BseMII	CTCAG	2 cut(s)	203, 249
BseNI	ACTGG	3 cut(s)	36, 730, 1147
BseRI	GAGGAG	2 cut(s)	140, 1200
BseXI	GCAGC	3 cut(s)	202, 724, 727
BshFI	GGCC	3 cut(s)	359, 443, 719
BshNI	GGYRCC	1 cut(s)	449
BsiHKAI	GWGCWC	1 cut(s)	8
BsiHKCI	CYCGRG	2 cut(s)	72, 167
BsiSI	CCGG	5 cut(s)	42, 89, 239, 579, 1111
BslFI	GGGAC	2 cut(s)	602, 840
BslI	CCNNNNNNNGG	3 cut(s)	174, 585, 1110
BsmAI	GTCTC	3 cut(s)	615, 764, 1074
BsmFI	GGGAC	2 cut(s)	602, 840
BsmI	GAATGC	1 cut(s)	1053
BsnI	GGCC	3 cut(s)	359, 443, 719
Bso31I	GGTCTC	2 cut(s)	764, 1074
BsoBI	CYCGRG	2 cut(s)	72, 167
Bsp1286I	GDGCHC	1 cut(s)	8
Bsp143I	GATC	8 cut(s)	133, 262, 346, 751, 836, 1015, 1021, 1099
BspACI	CCGC	3 cut(s)	278, 302, 1225
BspANI	GGCC	3 cut(s)	359, 443, 719
BspCNI	CTCAG	2 cut(s)	204, 250
BspLI	GGNNCC	5 cut(s)	451, 583, 590, 753, 1101
BspMAI	CTGCAG	1 cut(s)	609
BspMI	ACCTGC	1 cut(s)	1105
BspPI	GGATC	6 cut(s)	270, 746, 759, 1010, 1094, 1107
BspT107I	GGYRCC	1 cut(s)	449
BspTNI	GGTCTC	2 cut(s)	764, 1074
BsrFI	RCCGGY	2 cut(s)	41, 1110
BsrI	ACTGG	3 cut(s)	36, 730, 1147
BssAI	RCCGGY	2 cut(s)	41, 1110
BssECI	CCNNGG	3 cut(s)	445, 495, 585
BssMI	GATC	8 cut(s)	133, 262, 346, 751, 836, 1015, 1021, 1099
BssT1I	CCWWGG	2 cut(s)	445, 495
Bst2UI	CCWGG	2 cut(s)	586, 756
Bst4CI	ACNGT	4 cut(s)	680, 688, 910, 999
Bst6I	CTCTTC	1 cut(s)	768
BstC8I	GCNNGC	2 cut(s)	195, 1012
BstDEI	CTNAG	4 cut(s)	212, 258, 319, 383
BstF5I	GGATG	5 cut(s)	235, 310, 520, 529, 654
BstKTI	GATC	8 cut(s)	136, 265, 349, 754, 839, 1018, 1024, 1102
BstMAI	GTCTC	3 cut(s)	615, 764, 1074
BstMBI	GATC	8 cut(s)	133, 262, 346, 751, 836, 1015, 1021, 1099
BstMWI	GCNNNNNNNGC	7 cut(s)	47, 56, 178, 187, 449, 500, 857
BstNI	CCWGG	2 cut(s)	586, 756
BstNSI	RCATGY	4 cut(s)	494, 558, 833, 1014
BstSCI	CCNGG	2 cut(s)	584, 754
BstSFI	CTRYAG	1 cut(s)	605
BstV1I	GCAGC	3 cut(s)	202, 724, 727
BstV2I	GAAGAC	1 cut(s)	345
BstX2I	RGATCY	4 cut(s)	346, 751, 836, 1099
BstYI	RGATCY	4 cut(s)	346, 751, 836, 1099
BsuRI	GGCC	3 cut(s)	359, 443, 719
BtsCI	GGATG	5 cut(s)	235, 310, 520, 529, 654
BtsI	GCAGTG	1 cut(s)	21
BtsIMutI	CAGTG	3 cut(s)	21, 29, 995
BveI	ACCTGC	1 cut(s)	1105
Cac8I	GCNNGC	2 cut(s)	195, 1012
Cfr10I	RCCGGY	2 cut(s)	41, 1110
Cfr13I	GGNCC	4 cut(s)	392, 442, 589, 718
CseI	GACGC	1 cut(s)	35
Csp6I	GTAC	4 cut(s)	202, 538, 670, 1000
CviAII	CATG	8 cut(s)	491, 534, 555, 830, 980, 1011, 1238, 1254
CviQI	GTAC	4 cut(s)	202, 538, 670, 1000
DdeI	CTNAG	4 cut(s)	212, 258, 319, 383
DpnI	GATC	8 cut(s)	135, 264, 348, 753, 838, 1017, 1023, 1101
DpnII	GATC	8 cut(s)	133, 262, 346, 751, 836, 1015, 1021, 1099
DraI	TTTAAA	1 cut(s)	747
EaeI	YGGCCR	1 cut(s)	357
Eam1104I	CTCTTC	1 cut(s)	768
EarI	CTCTTC	1 cut(s)	768
Eco130I	CCWWGG	2 cut(s)	445, 495
Eco31I	GGTCTC	2 cut(s)	764, 1074
Eco47I	GGWCC	2 cut(s)	392, 589
Eco88I	CYCGRG	2 cut(s)	72, 167
EcoRI	GAATTC	2 cut(s)	529, 886
EcoRII	CCWGG	2 cut(s)	584, 754
EcoT14I	CCWWGG	2 cut(s)	445, 495
EcoT22I	ATGCAT	1 cut(s)	556
ErhI	CCWWGG	2 cut(s)	445, 495
FaeI	CATG	8 cut(s)	494, 537, 558, 833, 983, 1014, 1241, 1257
FalI	AAGNNNNNCTT	2 cut(s)	353, 385
FaqI	GGGAC	2 cut(s)	602, 840
FatI	CATG	8 cut(s)	490, 533, 554, 829, 979, 1010, 1237, 1253
Fnu4HI	GCNGC	3 cut(s)	191, 738, 741
FokI	GGATG	5 cut(s)	242, 317, 527, 536, 661
Fsp4HI	GCNGC	3 cut(s)	191, 738, 741
FspBI	CTAG	3 cut(s)	701, 809, 1094
GluI	GCNGC	3 cut(s)	191, 738, 741
GsuI	CTGGAG	1 cut(s)	53
HaeIII	GGCC	3 cut(s)	359, 443, 719
HapII	CCGG	5 cut(s)	42, 89, 239, 579, 1111
HgaI	GACGC	1 cut(s)	35
Hin1II	CATG	8 cut(s)	494, 537, 558, 833, 983, 1014, 1241, 1257
HincII	GTYRAC	2 cut(s)	416, 793
HindII	GTYRAC	2 cut(s)	416, 793
HindIII	AAGCTT	1 cut(s)	179
HinfI	GANTC	6 cut(s)	128, 155, 289, 373, 1064, 1207
HpaI	GTTAAC	1 cut(s)	793
HpaII	CCGG	5 cut(s)	42, 89, 239, 579, 1111
HphI	GGTGA	1 cut(s)	1205
Hpy166II	GTNNAC	3 cut(s)	416, 691, 793
Hpy188I	TCNGA	6 cut(s)	127, 133, 213, 925, 933, 1206
Hpy188III	TCNNGA	7 cut(s)	74, 167, 271, 662, 701, 800, 1019
Hpy8I	GTNNAC	3 cut(s)	416, 691, 793
Hpy99I	CGWCG	2 cut(s)	29, 104
HpyAV	CCTTC	3 cut(s)	334, 806, 1070
HpyCH4III	ACNGT	4 cut(s)	680, 688, 910, 999
HpyCH4V	TGCA	7 cut(s)	197, 330, 380, 554, 607, 851, 1241
HpyF10VI	GCNNNNNNNGC	7 cut(s)	47, 56, 178, 187, 449, 500, 857
HpyF3I	CTNAG	4 cut(s)	212, 258, 319, 383
Hsp92II	CATG	8 cut(s)	494, 537, 558, 833, 983, 1014, 1241, 1257
KspAI	GTTAAC	1 cut(s)	793
Kzo9I	GATC	8 cut(s)	133, 262, 346, 751, 836, 1015, 1021, 1099
LmnI	GCTCC	2 cut(s)	581, 997
Lsp1109I	GCAGC	3 cut(s)	202, 724, 727
LweI	GCATC	3 cut(s)	295, 860, 869
MaeI	CTAG	3 cut(s)	701, 809, 1094
MaeIII	GTNAC	2 cut(s)	323, 574
MalI	GATC	8 cut(s)	135, 264, 348, 753, 838, 1017, 1023, 1101
MboI	GATC	8 cut(s)	133, 262, 346, 751, 836, 1015, 1021, 1099
MboII	GAAGA	5 cut(s)	232, 345, 349, 785, 961
MfeI	CAATTG	1 cut(s)	1181
MflI	RGATCY	4 cut(s)	346, 751, 836, 1099
MhlI	GDGCHC	1 cut(s)	8
MluCI	AATT	7 cut(s)	529, 814, 880, 886, 1121, 1181, 1233
MlyI	GAGTC	2 cut(s)	367, 1201
Mph1103I	ATGCAT	1 cut(s)	556
MroXI	GAANNNNTTC	1 cut(s)	777
MseI	TTAA	7 cut(s)	68, 282, 675, 746, 792, 821, 975
MslI	CAYNNNNRTG	1 cut(s)	984
MspI	CCGG	5 cut(s)	42, 89, 239, 579, 1111
MspR9I	CCNGG	2 cut(s)	586, 756
MunI	CAATTG	1 cut(s)	1181
Mva1269I	GAATGC	1 cut(s)	1053
MvaI	CCWGG	2 cut(s)	586, 756
MwoI	GCNNNNNNNGC	7 cut(s)	47, 56, 178, 187, 449, 500, 857
NdeII	GATC	8 cut(s)	133, 262, 346, 751, 836, 1015, 1021, 1099
NlaIII	CATG	8 cut(s)	494, 537, 558, 833, 983, 1014, 1241, 1257
NlaIV	GGNNCC	5 cut(s)	451, 583, 590, 753, 1101
NmuCI	GTSAC	1 cut(s)	574
NsiI	ATGCAT	1 cut(s)	556
NspI	RCATGY	4 cut(s)	494, 558, 833, 1014
PaeI	GCATGC	1 cut(s)	1014
PaeR7I	CTCGAG	1 cut(s)	72
PctI	GAATGC	1 cut(s)	1053
PdmI	GAANNNNTTC	1 cut(s)	777
PfeI	GAWTC	4 cut(s)	128, 155, 289, 1064
PkrI	GCNGC	3 cut(s)	192, 739, 742
PleI	GAGTC	2 cut(s)	367, 1201
PpsI	GAGTC	2 cut(s)	367, 1201
Psp6I	CCWGG	2 cut(s)	584, 754
PspGI	CCWGG	2 cut(s)	584, 754
PspN4I	GGNNCC	5 cut(s)	451, 583, 590, 753, 1101
PspPI	GGNCC	4 cut(s)	392, 442, 589, 718
PsrI	GAACNNNNNNTAC	2 cut(s)	1020, 1052
PstI	CTGCAG	1 cut(s)	609
PsuI	RGATCY	4 cut(s)	346, 751, 836, 1099
RsaI	GTAC	4 cut(s)	203, 539, 671, 1001
RsaNI	GTAC	4 cut(s)	202, 538, 670, 1000
RseI	CAYNNNNRTG	1 cut(s)	984
SaqAI	TTAA	7 cut(s)	68, 282, 675, 746, 792, 821, 975
SatI	GCNGC	3 cut(s)	191, 738, 741
Sau3AI	GATC	8 cut(s)	133, 262, 346, 751, 836, 1015, 1021, 1099
Sau96I	GGNCC	4 cut(s)	392, 442, 589, 718
SchI	GAGTC	2 cut(s)	367, 1201
ScrFI	CCNGG	2 cut(s)	586, 756
SduI	GDGCHC	1 cut(s)	8
SfaNI	GCATC	3 cut(s)	295, 860, 869
SfcI	CTRYAG	1 cut(s)	605
Sfr274I	CTCGAG	1 cut(s)	72
SinI	GGWCC	2 cut(s)	392, 589
SlaI	CTCGAG	1 cut(s)	72
SmiMI	CAYNNNNRTG	1 cut(s)	984
SmlI	CTYRAG	1 cut(s)	72
SmoI	CTYRAG	1 cut(s)	72
SpeI	ACTAGT	1 cut(s)	1093
SphI	GCATGC	1 cut(s)	1014
Sse9I	AATT	7 cut(s)	529, 814, 880, 886, 1121, 1181, 1233
SsiI	CCGC	3 cut(s)	278, 302, 1225
SspMI	CTAG	3 cut(s)	701, 809, 1094
StyD4I	CCNGG	2 cut(s)	584, 754
StyI	CCWWGG	2 cut(s)	445, 495
TaaI	ACNGT	4 cut(s)	680, 688, 910, 999
TaqI	TCGA	5 cut(s)	73, 153, 272, 429, 1119
TasI	AATT	7 cut(s)	529, 814, 880, 886, 1121, 1181, 1233
TatI	WGTACW	2 cut(s)	669, 999
TfiI	GAWTC	4 cut(s)	128, 155, 289, 1064
Tru1I	TTAA	7 cut(s)	68, 282, 675, 746, 792, 821, 975
Tru9I	TTAA	7 cut(s)	68, 282, 675, 746, 792, 821, 975
TscAI	CASTG	3 cut(s)	21, 36, 1002
TseFI	GTSAC	1 cut(s)	574
TseI	GCWGC	3 cut(s)	190, 737, 740
Tsp45I	GTSAC	1 cut(s)	574
TspDTI	ATGAA	7 cut(s)	522, 657, 665, 726, 961, 996, 1226
TspRI	CASTG	3 cut(s)	21, 36, 1002
VpaK11BI	GGWCC	2 cut(s)	392, 589
XapI	RAATTY	4 cut(s)	529, 880, 886, 1121
XbaI	TCTAGA	1 cut(s)	700
XceI	RCATGY	4 cut(s)	494, 558, 833, 1014
XhoI	CTCGAG	1 cut(s)	72
XmnI	GAANNNNTTC	1 cut(s)	777
XspI	CTAG	3 cut(s)	701, 809, 1094
Zsp2I	ATGCAT	1 cut(s)	556

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.