MD17G1025700.v1.1

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000657
Physical Location & Seq
Forward (+)
1863824 .. 1868315
4492 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1025700.v1.1.491

Sequence Viewer

Length: 3738 bp
ATGGATTTGAACCCTGCTGGAGATCGAGGTTCGAATTATTTTGATAAAGTTCTGTCTATGGTTAGTTTTGCATACAATAAGGAGAGAGCAACCAATTCCTCGCCATTCATTGATTCCATTCTCCACAATGCTCACTTCCTTGTCTTTTTTGCTGTGTATCTTATTTGCCGTTATGTAAATCACATATCACTGTATCAAGCACTTGTTTGTGTCGGCCTTACCCCATTGTTTCTCTATTTCTACATCAACTATGCGCCGTTTCTGTTCTTAGAGGAAGACATCAAGTGGATTCGAAGAGAGTCGAGGTTACTGCATGCCATCGTTGAGGATGCAGAGAAAATCATGCATTATAGTGGTCTTATAGGCGCAAGAGCACGCAAAATCTGGGATTCTAATCCTGATGGAGCAACCGTGAAAGGAGTTGATGAAACTGAGGTTGCATTGTTGGATAAAGCCAAATCCATGGCTTTAAGAGCAAAAAATTTGGTTGCGACCTTTGAGAAACAGAGGATCGCAAGAGACTCCCACACCCAAAGATTATGTTTCGATATGATCACTGAAAATTTCCAGCAGGTATCAGAGCTGTTCACAGAAACTAAGACTGTTAAGCAGGGCATGAAGGATATCTTTGAGGGGAATAAGGATTACTGGATTGGCATTGGCGAATCTCTGGAGAGGTCAAGGTCCAGTATTATAAGCCTGCTGCCCAGGCCCGTGCATGGACATGGTCAACAAAAATGGGTGCAGTCCAAAGAACAAGTTTCCTCAGCAGCTGCTTTGGCGTCCACTGTCGAAGAGAACATAAAACGTCTCATCACTCAAAAGCCAGATTTAATGCGTAAAGATACCGGAAAGCAAATAATTTCAATAAACCTGCAACTGCAGCAACTGCATCATTTTCTGAAAGATGTACAAGGAATTCGATTCGAAAGTGAAATTGAAGGGGCCTGGGTGAAGGAAGTGCACGAGATGATTAATGAAGCACATCATGCCATTGACAAGTACTTGCAGAGAATTAGGTGGCTATCATTTATCAACAATTGGAAGGCCGGGAGAAAGCTCAAGGGGGACATAAGGTGTATACACGTTGGGTTCACACAACTCTTAGAAAGGAAAGATAGTTATGGCTTCAAGTTTTTAAGAAGAGATTCATCAAAATTCGTCTATCAGTCCCCACATCAGACTCAAGACGACTTAATTATTTCAACTAGATTAAGCAGGATGCAGGAGTTCCTCAGTAAGAAGTCACCTTTATTGGGTGAAGTATTTTATGAAGTCACTTCGTTGTGCAATCAATTGGAAAGAATGCATAAACTCGTACTTGGCGCATCAGTAACAGGAGGAGGATATAATTCCAGAATGTCTTGGTTGGAGCAAACCCGGAAAATTTTTGATAGTGCAGAGAAATCTTTGACTACCTTTCAGTGGAATTCACTCAATATGTGGCAAAACCCTCTATTTAAAACAAGTGCTGGGAGGAAACTTTCCCGTGACATTGACCTCATAAATCATACTATTGATCTTTTCTTGAAATGCATCAGAGCATATGACATTGAAGTAAGGGAAGAGTCAAACTCAGTGGTTGGTTTGGAAGAAGACACTGAAGCAGTGGTCTCACAACTGACAACCAACAACGAACACCGCTCGGTGATTTCCATTGTGGGGATTGGGGGCATAGGAAAGACAACACTGGCAAAGAAGATTTATGACAATGGAGTTGTTGTAGACCATTTTCCTTGCCGTGCCTGGGTTTCTATACCTCAGAAGTCTGATAACAAAGCACTTTTGGAAGATGTGGCAGAGCAGGTATTGAGGTCTCTTCAACTACAAGGGGAAAAAAATCGCAATGGATCCTGGATTCATAAGGCGCATGATGTCTTGAAAGACAAAAGGTACCTTTTAGTTCTTGACGACATCTCGACGGTAGAAGAATTGGAGACCCTGAAAGCAGAATTTCCAGTAACATCATCTGGATGCAAAATTCTGCTCACTACACGTGACAACATTGTGGCTTCACAAGCAGACGCTCCCCACCAACTTCGACTTCGAACCAAAGAAGAGAGCTGGAAGTTGTTCACCCAGATGGTGCACTTTCCGCCAACAGAGGAATTACTAGCAAAAGATATTTTAGATAAATGTGGGGGTTTACCACAAGCAATCTTCCATGTTGGATATCTGATGTTAGGGAAAGATGCGAGCGTGGCTGAGGAGGTCAAAGGGGTGCTTAGATACATCAAACAGAATGATGCACCATTGTTAGAAACCTTCACTATAAGGTCCATTCACTCGTCTCCTCATCTAAGAAACTGTCTTTCTTACTTCAAACTCTTCCCCAACAACTTTGAAATTCCGGCAAGGAGATTAACCGCATTGTGGATTGCAGAAGGTTTGGTAGGAGATCAGGAGACGGAAGAAACTGGCAAAGATGTTGCAGATAAGTATTTGTCCGACTTGATCCACCTGGACATGATTCAAGTAGTCAAAAGAAAGCACAACGGAGAAGTCAAGACATGTCGATTGCCATATGCTCTACAGAAATTTGAGTTGCCAGATATGAGCCAAGAAAGACTTACTGATCGTTATAACAGAAGCGACGAGACATTTACTCTTATTCATGGTGACAGCTCAAACGTTCCAATTCCTGATCTCGTTTCCATCCTCTCCTTTGATACTCGAGAAGGAAATAAACCTGGAGAAGAAATAGGCAACTTTCTACGAAGAGGCATCGCAGGTGGTTTCTTCCATCGGCTACAGGTTCTTGATCTCGAACGTGTATTCAGACCTGAATTGCCCAATACCATAGGAAAGCTAAAAGAGTTGAGGTATCTTGGTTTGAGGTGGACTTTCCTGGAATCAGTCCCAGCATCCATAGGTGACTTGCTGAACCTCCAAACCTTGGATGTGAAACATACATATGTGCACACTCTCCCTGGTTCAATATGGAAACTGCAGACACTTCGACACTTGTACTTGAACCAGAGTAAACAGAGTAAATTTAAGCATCAACAAGGTGGAAGTTCCCTGAAGAATCTGCGGACATTATGGGGAGTATTTCTGGATAAAGATAGTCCCTTGATCCACTGGATGGACAAGCTGATATACCTCAGAAAATTGGGATTGGCATTCCAGTTAAGCCAGCCAGAGCAAAAACCATTAGCAGACAAGATTGTTAAGCTGAAGAGCCTTCGGTCTTTGAGAATGAGATCAATCGATGAAATGGGTGAACCCAGTGATCTAGTCTGCGTGTGCTTATCAGGCCTTGAGAGTCTCTCCTGCCTAAATTTGTTCGGAAAGCTAAATCCTTCCATGATAGATGAATTCCCAAAAAATCTCACTGATCTTACGTTGTCAGGTGCTTCTCTGTCAGTTGATCCAATGCCCGTGTTAGAGAATCTCTCCAACCTGAAATTCCTCAGTTTTTACTCCAACTCTTATACAGGTATCAAGATGGTTTGCTCCACCACAGGGTTTCCAAAGCTTGTGGTTTTAAAGTTGTGGAAGCTCCAACAACTAAAAGATTGGGATGTGGAGGAAAAGGCAATGCAAAATCTGAGGGAGTTGGAGATAAGAGCGTGCACAAATTTGAAGGTCCCAAGTGGGTTGAGGCACTTGAAGAATCTCACTCAGCTGAAATTAACTAATATGCCGGAAGAATTTGTTGCAACCATTGCGACAACTAAGGTGCAAATTTGGGATGACATAACTCACTCGCCAACAGTCATTACCGATAGCTGGTAG

Protein Analysis

1246

Amino Acids

142.37

Weight (kDa)

8.54

Isoelectric Point (pI)

44.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 531 - 696 1.3e-36 NB-ARC domain
WHD_DRP PF23559 776 - 840 1.8e-12 Disease resistance protein Winged helix domain
LRR_14 PF23598 914 - 1213 4.4e-35 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000657)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g34770
malus_domestica MD17G1025700.v1.1
prunus_persica Prupe.3G040600_v2.0.a1 Prupe.3G040800_v2.0.a1 Prupe.3G041000_v2.0.a1 Prupe.3G041100_v2.0.a1 Prupe.3G041500_v2.0.a1 Prupe.4G109400_v2.0.a1 Prupe.8G037400_v2.0.a1 Prupe.8G037400_v2.0.a1 Prupe.8G037700_v2.0.a1
pyrus_communis pycom09g10040 pycom17g16000
rosa_chinensis RchiOBHm_Chr2g0145591 RchiOBHm_Chr2g0145611
rosa_laevigata RLG00000020146 RLG00000020149 RLG00000020151 RLG00000020154
rosa_multiflora Rmu_co7986250.1_g000001 Rmu_co8305779.1_g000001 Rmu_sc0002718.1_g000011 Rmu_sc0002835.1_g000009 Rmu_sc0002835.1_g000016 Rmu_sc0003046.1_g000007 Rmu_sc0003046.1_g000010 Rmu_sc0004445.1_g000006 Rmu_sc0004810.1_g000002 Rmu_sc0006477.1_g000008 Rmu_sc0014329.1_g000001 Rmu_sc0020976.1_g000003 Rmu_sc0021168.1_g000001 Rmu_sc0022199.1_g000001 Rmu_sc0036991.1_g000001 Rmu_sc0041497.1_g000003
rosa_roxburghii Rroxscaffold_152G00434560 Rroxscaffold_152G00434570 Rroxscaffold_152G00434600 Rroxscaffold_152G00434610 Rroxscaffold_2G00100630 Rroxscaffold_2G00100640 Rroxscaffold_2G00100660 Rroxscaffold_2G00100670 Rroxscaffold_2G00100680 Rroxscaffold_2G00100690
rosa_rugosa Rorug02G0392300 Rorug02G0392400 Rorug02G0392400
rosa_samantha Rh2AG445700 Rh2AG445800 Rh2AG446000 Rh2AG446100 Rh2AG446300 Rh2AG446600 Rh2BG457400 Rh2BG457500 Rh2BG457600 Rh2BG457900 Rh2BG458300 Rh2CG432300 Rh2CG432400 Rh2CG432500 Rh2CG432700 Rh2CG432900 Rh2CG433000 Rh2CG433300 Rh2DG467100 Rh2DG467200 Rh2DG467300 Rh2DG467600 Rh2DG467900 Rh3DG194100
rosa_wichuraiana Rw2G036420 Rw2G036450 Rw2G036470 Rw3G014900 Rw3G014920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 695, 2583
AarI CACCTGC 1 cut(s) 2720
Acc36I ACCTGC 4 cut(s) 562, 882, 1795, 2720
Acc65I GGTACC 1 cut(s) 1893
AccB1I GGYRCC 1 cut(s) 1893
AccB7I CCANNNNNTGG 2 cut(s) 2896, 3085
AccBSI CCGCTC 1 cut(s) 1644
AccI GTMKAC 2 cut(s) 1081, 1725
AciI CCGC 4 cut(s) 1642, 2096, 2367, 3034
AclI AACGTT 1 cut(s) 2631
AclWI GGATC 6 cut(s) 518, 1845, 1858, 2449, 3070, 3365
AcuI CTGAAG 3 cut(s) 1623, 3044, 3197
AcvI CACGTG 1 cut(s) 1997
AcyI GRCGYC 1 cut(s) 782
AfaI GTAC 5 cut(s) 912, 1004, 1320, 1895, 2969
AflIII ACRYGT 4 cut(s) 1084, 1994, 2510, 2770
AjnI CCWGG 8 cut(s) 707, 947, 1745, 1853, 2460, 2689, 2847, 2929
AjuI GAANNNNNNNTTGG 4 cut(s) 2028, 2060, 3392, 3424
Alw21I GWGCWC 5 cut(s) 376, 966, 2091, 2922, 3578
Alw26I GTCTC 9 cut(s) 513, 815, 1618, 1821, 1931, 2295, 2399, 2591, 3272
Alw44I GTGCAC 4 cut(s) 962, 2087, 2918, 3574
AlwI GGATC 6 cut(s) 518, 1845, 1858, 2449, 3070, 3365
AlwNI CAGNNNCTG 2 cut(s) 773, 889
Ama87I CYCGRG 1 cut(s) 2673
AoxI GGCC 5 cut(s) 214, 710, 945, 1047, 3256
ApaLI GTGCAC 4 cut(s) 962, 2087, 2918, 3574
ApeKI GCWGC 4 cut(s) 703, 770, 773, 883
AseI ATTAAT 1 cut(s) 975
Asp700I GAANNNNTTC 2 cut(s) 1147, 2072
Asp718I GGTACC 1 cut(s) 1893
AspLEI GCGC 4 cut(s) 256, 368, 1328, 1870
AspS9I GGNCC 5 cut(s) 684, 711, 945, 2277, 3589
AsuC2I CCSGG 2 cut(s) 1051, 1381
AsuHPI GGTGA 8 cut(s) 964, 1239, 1271, 1660, 2068, 2630, 2885, 3233
AsuII TTCGAA 4 cut(s) 32, 292, 927, 2047
AvaI CYCGRG 1 cut(s) 2673
AvaII GGWCC 3 cut(s) 684, 2277, 3589
BaeGI GKGCMC 4 cut(s) 966, 2091, 2922, 3578
BamHI GGATCC 1 cut(s) 1850
BanI GGYRCC 1 cut(s) 1893
BauI CACGAG 1 cut(s) 965
BbrPI CACGTG 1 cut(s) 1997
BbsI GAAGAC 2 cut(s) 282, 1602
Bbv12I GWGCWC 5 cut(s) 376, 966, 2091, 2922, 3578
BbvCI CCTCAGC 2 cut(s) 766, 2205
BbvI GCAGC 4 cut(s) 690, 760, 782, 895
BccI CCATC 7 cut(s) 326, 395, 2077, 2663, 2751, 3079, 3442
BceAI ACGGC 3 cut(s) 153, 241, 1725
BcgI CGANNNNNNTGC 4 cut(s) 292, 326, 2939, 2973
BciT130I CCWGG 8 cut(s) 709, 949, 1747, 1855, 2462, 2691, 2849, 2931
BclI TGATCA 1 cut(s) 552
BcnI CCSGG 2 cut(s) 1051, 1381
BcoDI GTCTC 9 cut(s) 513, 815, 1618, 1821, 1931, 2295, 2399, 2591, 3272
BfaI CTAG 3 cut(s) 1209, 2114, 3236
BfmI CTRYAG 4 cut(s) 881, 2531, 2750, 2948
BfuAI ACCTGC 4 cut(s) 562, 882, 1795, 2720
BisI GCNGC 4 cut(s) 704, 771, 774, 884
BlsI GCNGC 4 cut(s) 705, 772, 775, 885
BmcAI AGTACT 1 cut(s) 1004
Bme18I GGWCC 3 cut(s) 684, 2277, 3589
BmeT110I CYCGRG 1 cut(s) 2673
BmgT120I GGNCC 5 cut(s) 684, 711, 945, 2277, 3589
BmiI GGNNCC 4 cut(s) 946, 1852, 1895, 3591
BmrI ACTGGG 1 cut(s) 3222
BmuI ACTGGG 1 cut(s) 3222
BpiI GAAGAC 2 cut(s) 282, 1602
BplI GAGNNNNNCTC 6 cut(s) 1559, 1591, 3254, 3286, 3380, 3412
BpmI CTGGAG 3 cut(s) 39, 692, 2712
Bpu10I CCTNAGC 2 cut(s) 766, 2205
Bpu14I TTCGAA 4 cut(s) 32, 292, 927, 2047
BpuEI CTTGAG 3 cut(s) 1046, 1170, 3281
BpuMI CCSGG 2 cut(s) 1051, 1381
Bsa29I ATCGAT 1 cut(s) 3210
BsaAI YACGTR 1 cut(s) 1997
BsaBI GATNNNNATC 1 cut(s) 393
BsaHI GRCGYC 1 cut(s) 782
BsaI GGTCTC 3 cut(s) 1618, 1821, 1931
BsaJI CCNNGG 6 cut(s) 462, 707, 948, 1746, 2895, 2929
BsaWI WCCGGW 1 cut(s) 848
BsaXI ACNNNNNCTCC 4 cut(s) 396, 426, 1707, 1737
Bse1I ACTGG 8 cut(s) 653, 687, 1695, 1958, 2422, 3086, 3127, 3228
Bse3DI GCAATG 3 cut(s) 1852, 3546, 3666
Bse8I GATNNNNATC 1 cut(s) 393
BseBI CCWGG 8 cut(s) 709, 949, 1747, 1855, 2462, 2691, 2849, 2931
BseCI ATCGAT 1 cut(s) 3210
BseDI CCNNGG 6 cut(s) 462, 707, 948, 1746, 2895, 2929
BseGI GGATG 9 cut(s) 334, 1227, 1979, 2655, 2864, 2905, 3090, 3529, 3700
BseJI GATNNNNATC 1 cut(s) 393
BseMI GCAATG 3 cut(s) 1852, 3546, 3666
BseNI ACTGG 8 cut(s) 653, 687, 1695, 1958, 2422, 3086, 3127, 3228
BseRI GAGGAG 3 cut(s) 1356, 2222, 2283
BseSI GKGCMC 4 cut(s) 966, 2091, 2922, 3578
BseXI GCAGC 4 cut(s) 690, 760, 782, 895
BseYI CCCAGC 2 cut(s) 1472, 2860
BsgI GTGCAG 2 cut(s) 764, 1419
BshFI GGCC 5 cut(s) 216, 712, 947, 1049, 3258
BshNI GGYRCC 1 cut(s) 1893
BshVI ATCGAT 1 cut(s) 3210
BsiHKAI GWGCWC 5 cut(s) 376, 966, 2091, 2922, 3578
BsiHKCI CYCGRG 1 cut(s) 2673
BsiSI CCGG 5 cut(s) 849, 1050, 1381, 2351, 3647
BslFI GGGAC 5 cut(s) 1082, 1156, 2843, 3054, 3575
BsmAI GTCTC 9 cut(s) 513, 815, 1618, 1821, 1931, 2295, 2399, 2591, 3272
BsmBI CGTCTC 3 cut(s) 815, 2295, 2399
BsmFI GGGAC 5 cut(s) 1082, 1156, 2843, 3054, 3575
BsmI GAATGC 2 cut(s) 1311, 3122
BsnI GGCC 5 cut(s) 216, 712, 947, 1049, 3258
Bso31I GGTCTC 3 cut(s) 1618, 1821, 1931
BsoBI CYCGRG 1 cut(s) 2673
Bsp119I TTCGAA 4 cut(s) 32, 292, 927, 2047
Bsp1286I GDGCHC 5 cut(s) 376, 966, 2091, 2922, 3578
Bsp1407I TGTACA 1 cut(s) 910
Bsp19I CCATGG 1 cut(s) 462
BspACI CCGC 4 cut(s) 1642, 2096, 2367, 3034
BspANI GGCC 5 cut(s) 216, 712, 947, 1049, 3258
BspDI ATCGAT 1 cut(s) 3210
BspLI GGNNCC 4 cut(s) 946, 1852, 1895, 3591
BspMAI CTGCAG 2 cut(s) 885, 2952
BspMI ACCTGC 4 cut(s) 562, 882, 1795, 2720
BspPI GGATC 6 cut(s) 518, 1845, 1858, 2449, 3070, 3365
BspQI GCTCTTC 1 cut(s) 3173
BspT104I TTCGAA 4 cut(s) 32, 292, 927, 2047
BspT107I GGYRCC 1 cut(s) 1893
BspTNI GGTCTC 3 cut(s) 1618, 1821, 1931
BsrBI CCGCTC 1 cut(s) 1644
BsrDI GCAATG 3 cut(s) 1852, 3546, 3666
BsrGI TGTACA 1 cut(s) 910
BsrI ACTGG 8 cut(s) 653, 687, 1695, 1958, 2422, 3086, 3127, 3228
BssECI CCNNGG 6 cut(s) 462, 707, 948, 1746, 2895, 2929
BssNAI GTATAC 1 cut(s) 1082
BssNI GRCGYC 1 cut(s) 782
BssSI CACGAG 1 cut(s) 965
BssT1I CCWWGG 2 cut(s) 462, 2895
Bst1107I GTATAC 1 cut(s) 1082
Bst2BI CACGAG 1 cut(s) 965
Bst2UI CCWGG 8 cut(s) 709, 949, 1747, 1855, 2462, 2691, 2849, 2931
Bst4CI ACNGT 7 cut(s) 192, 412, 604, 790, 1924, 2309, 3718
Bst6I CTCTTC 9 cut(s) 289, 789, 1138, 1560, 1824, 2052, 2333, 2713, 3173
BstACI GRCGYC 1 cut(s) 782
BstAPI GCANNNNNTGC 3 cut(s) 889, 989, 3668
BstAUI TGTACA 1 cut(s) 910
BstBAI YACGTR 1 cut(s) 1997
BstBI TTCGAA 4 cut(s) 32, 292, 927, 2047
BstC8I GCNNGC 6 cut(s) 315, 376, 701, 2197, 3137, 3574
BstDSI CCRYGG 1 cut(s) 462
BstF5I GGATG 9 cut(s) 334, 1227, 1979, 2655, 2864, 2905, 3090, 3529, 3700
BstHHI GCGC 4 cut(s) 256, 368, 1328, 1870
BstMAI GTCTC 9 cut(s) 513, 815, 1618, 1821, 1931, 2295, 2399, 2591, 3272
BstNI CCWGG 8 cut(s) 709, 949, 1747, 1855, 2462, 2691, 2849, 2931
BstNSI RCATGY 2 cut(s) 317, 2514
BstSFI CTRYAG 4 cut(s) 881, 2531, 2750, 2948
BstSLI GKGCMC 4 cut(s) 966, 2091, 2922, 3578
BstV1I GCAGC 4 cut(s) 690, 760, 782, 895
BstV2I GAAGAC 2 cut(s) 282, 1602
BstX2I RGATCY 1 cut(s) 1850
BstXI CCANNNNNNTGG 1 cut(s) 463
BstYI RGATCY 1 cut(s) 1850
BstZ17I GTATAC 1 cut(s) 1082
Bsu15I ATCGAT 1 cut(s) 3210
BsuRI GGCC 5 cut(s) 216, 712, 947, 1049, 3258
BsuTUI ATCGAT 1 cut(s) 3210
BtgI CCRYGG 1 cut(s) 462
BtgZI GCGATG 1 cut(s) 2710
BtsCI GGATG 9 cut(s) 334, 1227, 1979, 2655, 2864, 2905, 3090, 3529, 3700
BtsI GCAGTG 1 cut(s) 1614
BveI ACCTGC 4 cut(s) 562, 882, 1795, 2720
Cac8I GCNNGC 6 cut(s) 315, 376, 701, 2197, 3137, 3574
CaiI CAGNNNCTG 2 cut(s) 773, 889
CfoI GCGC 4 cut(s) 256, 368, 1328, 1870
Cfr13I GGNCC 5 cut(s) 684, 711, 945, 2277, 3589
ClaI ATCGAT 1 cut(s) 3210
CseI GACGC 2 cut(s) 771, 2033
Csp6I GTAC 5 cut(s) 911, 1003, 1319, 1894, 2968
CspCI CAANNNNNGTGG 4 cut(s) 1560, 1595, 3462, 3497
CviQI GTAC 5 cut(s) 911, 1003, 1319, 1894, 2968
DraI TTTAAA 2 cut(s) 1462, 3489
Eam1104I CTCTTC 9 cut(s) 289, 789, 1138, 1560, 1824, 2052, 2333, 2713, 3173
EarI CTCTTC 9 cut(s) 289, 789, 1138, 1560, 1824, 2052, 2333, 2713, 3173
EciI GGCGGA 1 cut(s) 2085
Eco130I CCWWGG 2 cut(s) 462, 2895
Eco147I AGGCCT 1 cut(s) 3258
Eco31I GGTCTC 3 cut(s) 1618, 1821, 1931
Eco32I GATATC 2 cut(s) 625, 2174
Eco47I GGWCC 3 cut(s) 684, 2277, 3589
Eco57I CTGAAG 3 cut(s) 1623, 3044, 3197
Eco72I CACGTG 1 cut(s) 1997
Eco88I CYCGRG 1 cut(s) 2673
EcoO109I RGGNCCY 2 cut(s) 945, 3589
EcoRI GAATTC 3 cut(s) 918, 1429, 3317
EcoRII CCWGG 8 cut(s) 707, 947, 1745, 1853, 2460, 2689, 2847, 2929
EcoRV GATATC 2 cut(s) 625, 2174
EcoT14I CCWWGG 2 cut(s) 462, 2895
EcoT22I ATGCAT 3 cut(s) 348, 1311, 1538
ErhI CCWWGG 2 cut(s) 462, 2895
Esp3I CGTCTC 3 cut(s) 815, 2295, 2399
FalI AAGNNNNNCTT 6 cut(s) 611, 643, 2208, 2240, 2553, 2585
FaqI GGGAC 5 cut(s) 1082, 1156, 2843, 3054, 3575
FauNDI CATATG 3 cut(s) 1546, 2524, 2914
FbaI TGATCA 1 cut(s) 552
FblI GTMKAC 2 cut(s) 1081, 1725
Fnu4HI GCNGC 4 cut(s) 704, 771, 774, 884
FokI GGATG 9 cut(s) 341, 1234, 1986, 2642, 2851, 2912, 3097, 3536, 3707
Fsp4HI GCNGC 4 cut(s) 704, 771, 774, 884
FspBI CTAG 3 cut(s) 1209, 2114, 3236
GlaI GCGC 4 cut(s) 255, 367, 1327, 1869
GluI GCNGC 4 cut(s) 704, 771, 774, 884
GsaI CCCAGC 2 cut(s) 1476, 2864
GsuI CTGGAG 3 cut(s) 39, 692, 2712
HaeIII GGCC 5 cut(s) 216, 712, 947, 1049, 3258
HapII CCGG 5 cut(s) 849, 1050, 1381, 2351, 3647
HgaI GACGC 2 cut(s) 771, 2033
HhaI GCGC 4 cut(s) 256, 368, 1328, 1870
Hin1I GRCGYC 1 cut(s) 782
Hin6I GCGC 4 cut(s) 254, 366, 1326, 1868
HinP1I GCGC 4 cut(s) 254, 366, 1326, 1868
HincII GTYRAC 1 cut(s) 731
HindII GTYRAC 1 cut(s) 731
HindIII AAGCTT 1 cut(s) 3476
HpaII CCGG 5 cut(s) 849, 1050, 1381, 2351, 3647
HphI GGTGA 8 cut(s) 964, 1239, 1271, 1660, 2068, 2630, 2885, 3233
Hpy99I CGWCG 2 cut(s) 1924, 2597
HpyCH4III ACNGT 7 cut(s) 192, 412, 604, 790, 1924, 2309, 3718
HpyCH4IV ACGT 6 cut(s) 808, 1086, 1996, 2631, 2770, 3344
HpySE526I ACGT 6 cut(s) 808, 1086, 1996, 2631, 2770, 3344
Hsp92I GRCGYC 1 cut(s) 782
HspAI GCGC 4 cut(s) 254, 366, 1326, 1868
KpnI GGTACC 1 cut(s) 1897
Ksp22I TGATCA 1 cut(s) 552
LguI GCTCTTC 1 cut(s) 3173
LmnI GCTCC 5 cut(s) 404, 1372, 2032, 3461, 3507
Lsp1109I GCAGC 4 cut(s) 690, 760, 782, 895
MaeI CTAG 3 cut(s) 1209, 2114, 3236
MaeII ACGT 6 cut(s) 808, 1086, 1996, 2631, 2770, 3344
MaeIII GTNAC 9 cut(s) 306, 1245, 1276, 1333, 1490, 1960, 1997, 2618, 2873
MbiI CCGCTC 1 cut(s) 1644
MfeI CAATTG 2 cut(s) 1039, 1295
MflI RGATCY 1 cut(s) 1850
MhlI GDGCHC 5 cut(s) 376, 966, 2091, 2922, 3578
MlyI GAGTC 5 cut(s) 308, 515, 1177, 1577, 3274
MmeI TCCRAC 8 cut(s) 426, 1350, 2149, 2472, 3423, 3450, 3529, 3540
Mph1103I ATGCAT 3 cut(s) 348, 1311, 1538
MroXI GAANNNNTTC 2 cut(s) 1147, 2072
MslI CAYNNNNRTG 5 cut(s) 351, 723, 1972, 2081, 2913
MspA1I CMGCKG 2 cut(s) 773, 3628
MspI CCGG 5 cut(s) 849, 1050, 1381, 2351, 3647
MunI CAATTG 2 cut(s) 1039, 1295
Mva1269I GAATGC 2 cut(s) 1311, 3122
MvaI CCWGG 8 cut(s) 709, 949, 1747, 1855, 2462, 2691, 2849, 2931
NciI CCSGG 2 cut(s) 1051, 1381
NcoI CCATGG 1 cut(s) 462
NdeI CATATG 3 cut(s) 1546, 2524, 2914
NlaIV GGNNCC 4 cut(s) 946, 1852, 1895, 3591
NmuCI GTSAC 6 cut(s) 1245, 1276, 1490, 1997, 2618, 2873
NsiI ATGCAT 3 cut(s) 348, 1311, 1538
NspI RCATGY 2 cut(s) 317, 2514
NspV TTCGAA 4 cut(s) 32, 292, 927, 2047
PaeI GCATGC 1 cut(s) 317
PaeR7I CTCGAG 1 cut(s) 2673
PaqCI CACCTGC 1 cut(s) 2720
PceI AGGCCT 1 cut(s) 3258
PciI ACATGT 1 cut(s) 2510
PciSI GCTCTTC 1 cut(s) 3173
PctI GAATGC 2 cut(s) 1311, 3122
PdmI GAANNNNTTC 2 cut(s) 1147, 2072
PflFI GACNNNGTC 1 cut(s) 726
PflMI CCANNNNNTGG 2 cut(s) 2896, 3085
PfoI TCCNGGA 2 cut(s) 1853, 2847
PkrI GCNGC 4 cut(s) 705, 772, 775, 885
PleI GAGTC 5 cut(s) 307, 515, 1177, 1576, 3273
PmaCI CACGTG 1 cut(s) 1997
PmlI CACGTG 1 cut(s) 1997
PpsI GAGTC 5 cut(s) 307, 515, 1177, 1576, 3273
Ppu21I YACGTR 1 cut(s) 1997
PpuMI RGGWCCY 1 cut(s) 3589
PscI ACATGT 1 cut(s) 2510
PshBI ATTAAT 1 cut(s) 975
PsiI TTATAA 2 cut(s) 695, 2583
Psp1406I AACGTT 1 cut(s) 2631
Psp5II RGGWCCY 1 cut(s) 3589
Psp6I CCWGG 8 cut(s) 707, 947, 1745, 1853, 2460, 2689, 2847, 2929
PspCI CACGTG 1 cut(s) 1997
PspFI CCCAGC 2 cut(s) 1472, 2860
PspGI CCWGG 8 cut(s) 707, 947, 1745, 1853, 2460, 2689, 2847, 2929
PspN4I GGNNCC 4 cut(s) 946, 1852, 1895, 3591
PspPI GGNCC 5 cut(s) 684, 711, 945, 2277, 3589
PspPPI RGGWCCY 1 cut(s) 3589
PstI CTGCAG 2 cut(s) 885, 2952
PstNI CAGNNNCTG 2 cut(s) 773, 889
PsuI RGATCY 1 cut(s) 1850
PsyI GACNNNGTC 1 cut(s) 726
PvuII CAGCTG 2 cut(s) 773, 3628
RsaI GTAC 5 cut(s) 912, 1004, 1320, 1895, 2969
RsaNI GTAC 5 cut(s) 911, 1003, 1319, 1894, 2968
RseI CAYNNNNRTG 5 cut(s) 351, 723, 1972, 2081, 2913
SapI GCTCTTC 1 cut(s) 3173
SatI GCNGC 4 cut(s) 704, 771, 774, 884
Sau96I GGNCC 5 cut(s) 684, 711, 945, 2277, 3589
ScaI AGTACT 1 cut(s) 1004
SchI GAGTC 5 cut(s) 308, 515, 1177, 1577, 3274
SduI GDGCHC 5 cut(s) 376, 966, 2091, 2922, 3578
SfcI CTRYAG 4 cut(s) 881, 2531, 2750, 2948
Sfr274I CTCGAG 1 cut(s) 2673
SfuI TTCGAA 4 cut(s) 32, 292, 927, 2047
SinI GGWCC 3 cut(s) 684, 2277, 3589
SlaI CTCGAG 1 cut(s) 2673
SmiMI CAYNNNNRTG 5 cut(s) 351, 723, 1972, 2081, 2913
SmlI CTYRAG 4 cut(s) 1061, 1185, 2673, 3260
SmoI CTYRAG 4 cut(s) 1061, 1185, 2673, 3260
SphI GCATGC 1 cut(s) 317
SseBI AGGCCT 1 cut(s) 3258
SsiI CCGC 4 cut(s) 1642, 2096, 2367, 3034
SspMI CTAG 3 cut(s) 1209, 2114, 3236
StuI AGGCCT 1 cut(s) 3258
StyI CCWWGG 2 cut(s) 462, 2895
TaaI ACNGT 7 cut(s) 192, 412, 604, 790, 1924, 2309, 3718
TaiI ACGT 6 cut(s) 811, 1089, 1999, 2634, 2773, 3347
TaqII GACCGA 1 cut(s) 3177
TatI WGTACW 3 cut(s) 910, 1002, 2967
TseFI GTSAC 6 cut(s) 1245, 1276, 1490, 1997, 2618, 2873
TseI GCWGC 4 cut(s) 703, 770, 773, 883
Tsp45I GTSAC 6 cut(s) 1245, 1276, 1490, 1997, 2618, 2873
TspGWI ACGGA 2 cut(s) 2423, 2511
Tth111I GACNNNGTC 1 cut(s) 726
Van91I CCANNNNNTGG 2 cut(s) 2896, 3085
VneI GTGCAC 4 cut(s) 962, 2087, 2918, 3574
VpaK11BI GGWCC 3 cut(s) 684, 2277, 3589
VspI ATTAAT 1 cut(s) 975
XceI RCATGY 2 cut(s) 317, 2514
XhoI CTCGAG 1 cut(s) 2673
XmiI GTMKAC 2 cut(s) 1081, 1725
XmnI GAANNNNTTC 2 cut(s) 1147, 2072
XspI CTAG 3 cut(s) 1209, 2114, 3236
ZrmI AGTACT 1 cut(s) 1004
Zsp2I ATGCAT 3 cut(s) 348, 1311, 1538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.