Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0000657

Physical Location & Seq

Genomic Coordinates Forward (+)

61791473 .. 61795057

Gene Structure 3585 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000020151

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3585 bp

ATGGCGGACCACATATTTGCCATCTTTTGCATCCTGTTTGTCATTGTCGTTATTCTTTACCTTGTCACAAGTGAAGGTGCTTTCAGATACTCGGACGGGAAGTACGAGTTGGTTAGAAGAGAACTGAGGTTAATGCATGCTCTGTTAAAAGATTGCGAGAAAGTGTCCAGGTCAGCCGAACTAGTAACTCGAACAGCCAGTCAGTTTTCGGATTCCGATCAAATCCAAAATGGAAAGAGTGCACAAGTACTAAATGAGAGCGAGAAAGGCTGGATAAAGAAAGCAAGAGAATCAGTTATTGATGCAACTCCGTGCCTCCAAAGATTTGAAAATTTGAGGAACAGAAGAAATGCCAAATGGTTGCTCTCCATCGTGCTTGATATCGCACCGAGGTTTGATGCTGCCCTTAAGATGAAGCGGTTCGACGAAACCATAAATGGTAACCTTGGAGTGGATCAGAGCGGGAAGAAAGTTTGTGCCAGTGAAATCAATAAATCAGTGGAGCAGTCAAGATCCAAGATCAGAAGCCTGCTAGACAGATCTAGTGTACATGAGGTTGAATCTGCCAAGTTCAGACCACCTGTTGGCACAATAAAAGCTGATGAATTAACTTCGTCTCTGAAGACTTTGAGCACTAGACGCCCATATTTGATGCAGGGCCAGCTTGAGGAGCACATAAAATCCACCATGATGCATTTACAGCTACTTCAAGCTTTCATGGAAGATGTACACAGCCTCGAAGTGCTGGAAAGTGAAATGGAAAACACCTGGGTGATGGAAGTGAACGACACCATTATTCAAGCGAAGGATGTGATTGACACCATAAATGCCACAGAAAACAAGAGAGGATGGATTTCAGAACCGAATAATTGGATTCGTAGGTGGAAGCATAGGGAGGATATAGTGTCTGTTGGCACCCGGATCTCTGGCCTCCTCACAACAAAGGAAAGATTTGGTGTCAATTTCATCAAATTTATTGAGTCATCAAAATCTGCCCACCACTCTCCTCAGCACCAAGCCTTTCAGCATCCAACTGAACCTGATATTGGCTTGTTGGATGCAGTCAACAACATCCGTAACTTGCTCGATCAACTGCAAAAGACGAGCGCCAAGTTAGTATCTCAAGTCAGCAAAGTGAATGAGGAATTGGATAAACTTTTCGAATATACAAAGGAAACTGAAGAGTATGCTGTGAATCTGAGAAATGCTTCCTGGAAGCTGTTGAAGAAAACCGCTTCAGAAGCAAACGAGCTGTACCAGAAAACCACTTCGAAGTTTCTATCAAAAACAATACTTGTTCGCATCAAGAATGCTGTCAATGTTCTTGAGAGATGCCTGAAAGCATATTCCATTGACGCAAGGGAGGAGTCATGTGCAGTAGTTGGTTTGGACGAAGACATACACGAACTGGTCTCGAGACTCACTACTAATAGTGAACCTGCTAGTTCTATCATACCCATTGTGGGGATGAAGGGCATAGGTAAGACCACTCTAGCCAAGCAAGTTTACTACCATGCTAACATTGTGGAGCACTTTCCAGTCCGTCGTTGGGTATCCCTACCTCCATACCAAAACTCTGATGAATTTGCATTTTTAAGAAGTGTTGGGATCCAGGTCCGGGAGACCAAAGAGAAATATTCGAATGCATACGAATCAAAAGCCTACTGGATCAACAGAGTGCGCAATTTCTTGAACATGAACATGTTCCTTTTAGTATTGGACAATGTCGTGTCCATGGAAGTCTGGGATTCTCTCAAACAAGCACTGCCAGAAAGGACTAATGGGAGCAAGATTGTGCTGATCACACGCAACAAGGCAATTGCTTTAAATGCTGACCGAGATAGCATTCCACACCAACTGCGGCTACGAACTCAAGAAGAGAGCTGGGAGCTTTTCTCTCAGATAGTGCTAGTGCAGTACAATGATCCCGAGAAAAAGGAAACCAATCGTGTGAAGGAAGTTGTAGGAAGCAGCTTTGGAGGCTTACCACTTGACATTATAGGCTCTGGCAGTTTACTTTGGGGGAAACAAATCACAACCGAAGAAATTTTGAGGGTTCTTGGACGTAGCACTACTCAAGGACAGGACCAAAATCCTTGGTCACTGAACAAGGCAGTAAATGAAGACGACTTGCAAGAGAATCAGAGTTTTAGGCACTGTTTGTCTTACTTCCAGCTCTTCCCGCCGGACTTTGAAATCCCTGTCAGGAGAATAGTGGCTTGGTGGGTTGCAAGAGAGCTGGAAGGAATCAATGAGAATCATATTCCTGAAAACTGGGTTACTCAACGGTTGAGACGAGTTAATGAGATTCGTATTCCTGAAACTCTTGCGAATGAGTATCTACAAGAGTTGATCGGCCGTAACATGATTCAAGTAGTCAAGAAAAAGCCTGATGGGAAGACCAAGACATGTCGATTGCCTAGTTCTATGGGAGCTCTGTTGTTGCAAGGCCAAGGCGTTAATAGCAGCGAAACTGATCCCTCTGAATCATCTCCTGATCAAGATCATGGTCCTACCGGGACAAATACACAGCAGAATGAAAGCGAACACAAGTCCATTATCTTCTTCGAGACTGTAGAGCGAAATAAACCGAATCAAGAAATATGTGACTTCCTACGCAAAGGCATTGCTGATGGTTTCTTGGGACAGCTACAATTTCTTGACCTTGAACGTGTATTCCAACCACTGTTGCCGAAGGCCATTGGAAATTTAAAGCTGCTGGTATATCTGGGCTTAAGGTGGACTTACCTTGAGTCGATTCCAAAATCTATAGGCCAGTTGGTCGAACTCCAGACCCTTGATTTGAAGCATACTTACATTCAGCACAATCTTCCTAGTTCAATCTGGAAACTGAAGAAACTTCGGTATCTCTACTTAAATCACAACTGTCAATTGCCACAATTGACTCGGTTGATATTATTGCCTCGATCAAATTCCATTGGCATGGAGGATCTGCAGAAATTGTCTGGTGTGTTTGTGGATAATGTAAATCTGGTTAAGGATGGGCTTGGAAAGTTGATCAACCTTAGAAAATTGGAGTTGGCATTCCAGCTTGACACTTCGGACGAGCAAGATGTAGTGGAAAGTTGGATTGCGAAGCTCAAGTTCTTAGAGTCTTTGAAGTTGAAATCAATTGATAAGAAGAGGAAACTTCGGGATCTGAAATTACAGTTTCTCCCAACCCTTGAGCTACTGTCCCGGTTACATTTGTTTGGAAAGCTTGTAAATCGACAAATCATCAACCAAGTTCCTCAAAGCCTAACTCATCTTACCCTCTCTGCCTCTGAAATTGAAGACGATCCAATGCCGGTGCTAGGCACGCTTCAGAATCTCATATCGCTATCTTTCTACTCTGGTTCTTATCTTGGAAGACGCATGGTTTGCCGCATGAACGGGTTTCCGAAGCTTGTAGTTCTGAAGCTTTGTAAGCTTGATGCCCTGGAAATATTGGATTTGCAAGAAGCAATGCCAAAGCTAAGCAATTTAGTGATTAAATATTCCAAGAAGCCCAAAGTGACTGTTGTTGGATTGACGCGTTTAACCACCGTCCAAGAATTTGAACAATCAACTGCAGCCGAGGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0002376 GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008219 GO:0009506 GO:0009626 GO:0009987 GO:0012501 GO:0016020 GO:0019897 GO:0019898 GO:0023052 GO:0030054 GO:0033554 GO:0034050 GO:0036094 GO:0044425 GO:0044459 GO:0044464 GO:0045087 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0065007 GO:0071944 GO:0097159 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRR_8 NB-ARC

Protein Analysis

1195

Amino Acids

136.58

Weight (kDa)

8.68

Isoelectric Point (pI)

45.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rx_N	PF18052	215 - 283	6.7e-06	Rx N-terminal domain
NB-ARC	PF00931	465 - 637	4.7e-31	NB-ARC domain
LRR_14	PF23598	885 - 1184	3.9e-34	Leucine-rich repeat region
LRR_8	PF13855	907 - 962	8.7e-07	Leucine rich repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0000657)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g34770
malus_domestica	MD17G1025700.v1.1
prunus_persica	Prupe.3G040600_v2.0.a1 Prupe.3G040800_v2.0.a1 Prupe.3G041000_v2.0.a1 Prupe.3G041100_v2.0.a1 Prupe.3G041500_v2.0.a1 Prupe.4G109400_v2.0.a1 Prupe.8G037400_v2.0.a1 Prupe.8G037400_v2.0.a1 Prupe.8G037700_v2.0.a1
pyrus_communis	pycom09g10040 pycom17g16000
rosa_chinensis	RchiOBHm_Chr2g0145591 RchiOBHm_Chr2g0145611
rosa_laevigata	RLG00000020146 RLG00000020149 RLG00000020151 RLG00000020154
rosa_multiflora	Rmu_co7986250.1_g000001 Rmu_co8305779.1_g000001 Rmu_sc0002718.1_g000011 Rmu_sc0002835.1_g000009 Rmu_sc0002835.1_g000016 Rmu_sc0003046.1_g000007 Rmu_sc0003046.1_g000010 Rmu_sc0004445.1_g000006 Rmu_sc0004810.1_g000002 Rmu_sc0006477.1_g000008 Rmu_sc0014329.1_g000001 Rmu_sc0020976.1_g000003 Rmu_sc0021168.1_g000001 Rmu_sc0022199.1_g000001 Rmu_sc0036991.1_g000001 Rmu_sc0041497.1_g000003
rosa_roxburghii	Rroxscaffold_152G00434560 Rroxscaffold_152G00434570 Rroxscaffold_152G00434600 Rroxscaffold_152G00434610 Rroxscaffold_2G00100630 Rroxscaffold_2G00100640 Rroxscaffold_2G00100660 Rroxscaffold_2G00100670 Rroxscaffold_2G00100680 Rroxscaffold_2G00100690
rosa_rugosa	Rorug02G0392300 Rorug02G0392400 Rorug02G0392400
rosa_samantha	Rh2AG445700 Rh2AG445800 Rh2AG446000 Rh2AG446100 Rh2AG446300 Rh2AG446600 Rh2BG457400 Rh2BG457500 Rh2BG457600 Rh2BG457900 Rh2BG458300 Rh2CG432300 Rh2CG432400 Rh2CG432500 Rh2CG432700 Rh2CG432900 Rh2CG433000 Rh2CG433300 Rh2DG467100 Rh2DG467200 Rh2DG467300 Rh2DG467600 Rh2DG467900 Rh3DG194100
rosa_wichuraiana	Rw2G036420 Rw2G036450 Rw2G036470 Rw3G014900 Rw3G014920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1682
Acc36I	ACCTGC	1 cut(s)	1447
AccB1I	GGYRCC	1 cut(s)	914
AccB7I	CCANNNNNTGG	1 cut(s)	584
AccBSI	CCGCTC	1 cut(s)	462
AccII	CGCG	1 cut(s)	3535
AciI	CCGC	7 cut(s)	5, 418, 462, 1233, 1861, 2183, 3385
AcoI	YGGCCR	1 cut(s)	2356
AcsI	RAATTY	7 cut(s)	331, 971, 1583, 2046, 2707, 2932, 3554
AcuI	CTGAAG	6 cut(s)	641, 1200, 1221, 2873, 3308, 3437
AcyI	GRCGYC	1 cut(s)	640
AfaI	GTAC	6 cut(s)	104, 249, 549, 729, 1256, 1919
AfiI	CCNNNNNNNGG	9 cut(s)	451, 584, 667, 1046, 1463, 1464, 1549, 1618, 3314
AflII	CTTAAG	2 cut(s)	407, 2734
AflIII	ACRYGT	4 cut(s)	1701, 2408, 2671, 3533
AhlI	ACTAGT	1 cut(s)	181
AjnI	CCWGG	5 cut(s)	167, 767, 1211, 1611, 3438
AjuI	GAANNNNNNNTTGG	8 cut(s)	92, 124, 1029, 1061, 1130, 1162, 2203, 2235
AleI	CACNNNNGTG	1 cut(s)	770
AloI	GAACNNNNNNTCC	2 cut(s)	2661, 2693
Alw21I	GWGCWC	5 cut(s)	244, 635, 675, 1533, 2437
Alw26I	GTCTC	6 cut(s)	621, 1411, 1417, 1616, 2287, 2564
Alw44I	GTGCAC	1 cut(s)	240
Ama87I	CYCGRG	2 cut(s)	1414, 1928
AoxI	GGCC	6 cut(s)	658, 928, 2356, 2449, 2697, 2773
ApaLI	GTGCAC	1 cut(s)	240
ApeKI	GCWGC	5 cut(s)	401, 1971, 2466, 2716, 3572
ApoI	RAATTY	7 cut(s)	331, 971, 1583, 2046, 2707, 2932, 3554
ArsI	GACNNNNNNTTYG	7 cut(s)	31, 184, 216, 2785, 2817, 3363, 3395
Asp700I	GAANNNNTTC	3 cut(s)	419, 1207, 1703
AspLEI	GCGC	2 cut(s)	1109, 1683
AspS9I	GGNCC	5 cut(s)	7, 658, 1615, 2086, 2510
AsuC2I	CCSGG	4 cut(s)	919, 1619, 2518, 3199
AsuHPI	GGTGA	1 cut(s)	784
AsuII	TTCGAA	3 cut(s)	1161, 1271, 1640
AvaI	CYCGRG	2 cut(s)	1414, 1928
AvaII	GGWCC	4 cut(s)	7, 1615, 2086, 2510
BaeGI	GKGCMC	1 cut(s)	244
BaeI	ACNNNNGTAYC	2 cut(s)	2849, 2882
BamHI	GGATCC	1 cut(s)	1608
BanI	GGYRCC	1 cut(s)	914
BanII	GRGCYC	1 cut(s)	2437
BarI	GAAGNNNNNNTAC	2 cut(s)	2798, 2830
BbsI	GAAGAC	6 cut(s)	629, 1401, 2130, 2405, 3300, 3376
Bbv12I	GWGCWC	5 cut(s)	244, 635, 675, 1533, 2437
BbvCI	CCTCAGC	1 cut(s)	1008
BbvI	GCAGC	4 cut(s)	388, 1983, 2478, 2703
BccI	CCATC	7 cut(s)	29, 377, 769, 843, 2387, 2627, 2996
BceAI	ACGGC	1 cut(s)	2343
BciT130I	CCWGG	5 cut(s)	169, 769, 1213, 1613, 3440
BciVI	GTATCC	1 cut(s)	1564
BclI	TGATCA	3 cut(s)	1800, 2497, 3018
BcnI	CCSGG	4 cut(s)	919, 1619, 2518, 3199
BcoDI	GTCTC	6 cut(s)	621, 1411, 1417, 1616, 2287, 2564
BcuI	ACTAGT	1 cut(s)	181
BfmI	CTRYAG	4 cut(s)	2574, 2769, 2954, 3570
BfoI	RGCGCY	1 cut(s)	1110
BfrI	CTTAAG	2 cut(s)	407, 2734
BfuAI	ACCTGC	1 cut(s)	1447
BfuI	GTATCC	1 cut(s)	1564
BglII	AGATCT	1 cut(s)	539
BisI	GCNGC	7 cut(s)	402, 1862, 1972, 2467, 2717, 3385, 3573
BlpI	GCTNAGC	1 cut(s)	3476
BlsI	GCNGC	7 cut(s)	403, 1863, 1973, 2468, 2718, 3386, 3574
BmcAI	AGTACT	1 cut(s)	249
Bme1390I	CCNGG	9 cut(s)	169, 769, 919, 1213, 1613, 1619, 2518, 3199, 3440
Bme18I	GGWCC	4 cut(s)	7, 1615, 2086, 2510
BmeT110I	CYCGRG	2 cut(s)	1414, 1928
BmgT120I	GGNCC	5 cut(s)	7, 658, 1615, 2086, 2510
BmiI	GGNNCC	2 cut(s)	916, 1610
BmrFI	CCNGG	9 cut(s)	169, 769, 919, 1213, 1613, 1619, 2518, 3199, 3440
BmrI	ACTGGG	1 cut(s)	2284
BmuI	ACTGGG	1 cut(s)	2284
BpiI	GAAGAC	6 cut(s)	629, 1401, 2130, 2405, 3300, 3376
BplI	GAGNNNNNCTC	2 cut(s)	1880, 1912
BpmI	CTGGAG	1 cut(s)	2774
Bpu10I	CCTNAGC	1 cut(s)	1008
Bpu1102I	GCTNAGC	1 cut(s)	3476
Bpu14I	TTCGAA	3 cut(s)	1161, 1271, 1640
BpuEI	CTTGAG	8 cut(s)	686, 1107, 1346, 1857, 2061, 2771, 3086, 3206
BpuMI	CCSGG	4 cut(s)	919, 1619, 2518, 3199
BsaBI	GATNNNNATC	2 cut(s)	216, 2502
BsaHI	GRCGYC	1 cut(s)	640
BsaI	GGTCTC	2 cut(s)	1417, 1616
BsaJI	CCNNGG	8 cut(s)	389, 445, 768, 1734, 2096, 2452, 3438, 3576
BsaXI	ACNNNNNCTCC	2 cut(s)	3159, 3189
Bsc4I	CCNNNNNNNGG	9 cut(s)	451, 584, 667, 1046, 1463, 1464, 1549, 1618, 3314
Bse118I	RCCGGY	1 cut(s)	3307
Bse1I	ACTGG	7 cut(s)	198, 480, 1413, 1538, 1670, 2279, 2776
Bse3DI	GCAATG	2 cut(s)	2625, 3471
Bse8I	GATNNNNATC	2 cut(s)	216, 2502
BseBI	CCWGG	5 cut(s)	169, 769, 1213, 1613, 3440
BseDI	CCNNGG	8 cut(s)	389, 445, 768, 1734, 2096, 2452, 3438, 3576
BseGI	GGATG	8 cut(s)	30, 814, 854, 1027, 1063, 1071, 1473, 3007
BseJI	GATNNNNATC	2 cut(s)	216, 2502
BseLI	CCNNNNNNNGG	9 cut(s)	451, 584, 667, 1046, 1463, 1464, 1549, 1618, 3314
BseMI	GCAATG	2 cut(s)	2625, 3471
BseMII	CTCAG	4 cut(s)	116, 1022, 1190, 1913
BseNI	ACTGG	7 cut(s)	198, 480, 1413, 1538, 1670, 2279, 2776
BseRI	GAGGAG	4 cut(s)	683, 923, 996, 1379
BseSI	GKGCMC	1 cut(s)	244
BseX3I	CGGCCG	1 cut(s)	2356
BseXI	GCAGC	4 cut(s)	388, 1983, 2478, 2703
BseYI	CCCAGC	1 cut(s)	1884
BsgI	GTGCAG	2 cut(s)	1395, 1934
Bsh1236I	CGCG	1 cut(s)	3535
Bsh1285I	CGRYCG	1 cut(s)	2359
BshFI	GGCC	6 cut(s)	660, 930, 2358, 2451, 2699, 2775
BshNI	GGYRCC	1 cut(s)	914
BsiEI	CGRYCG	1 cut(s)	2359
BsiHKAI	GWGCWC	5 cut(s)	244, 635, 675, 1533, 2437
BsiHKCI	CYCGRG	2 cut(s)	1414, 1928
BsiSI	CCGG	6 cut(s)	919, 1618, 2186, 2517, 3199, 3308
BslFI	GGGAC	3 cut(s)	2533, 2658, 3181
BslI	CCNNNNNNNGG	9 cut(s)	451, 584, 667, 1046, 1463, 1464, 1549, 1618, 3314
BsmAI	GTCTC	6 cut(s)	621, 1411, 1417, 1616, 2287, 2564
BsmBI	CGTCTC	2 cut(s)	621, 2287
BsmFI	GGGAC	3 cut(s)	2533, 2658, 3181
BsmI	GAATGC	4 cut(s)	1315, 1648, 1845, 3044
BsnI	GGCC	6 cut(s)	660, 930, 2358, 2451, 2699, 2775
Bso31I	GGTCTC	2 cut(s)	1417, 1616
BsoBI	CYCGRG	2 cut(s)	1414, 1928
Bsp119I	TTCGAA	3 cut(s)	1161, 1271, 1640
Bsp1286I	GDGCHC	5 cut(s)	244, 635, 675, 1533, 2437
Bsp1407I	TGTACA	2 cut(s)	547, 727
Bsp1720I	GCTNAGC	1 cut(s)	3476
Bsp19I	CCATGG	1 cut(s)	1734
BspACI	CCGC	7 cut(s)	5, 418, 462, 1233, 1861, 2183, 3385
BspANI	GGCC	6 cut(s)	660, 930, 2358, 2451, 2699, 2775
BspCNI	CTCAG	4 cut(s)	117, 1021, 1191, 1912
BspFNI	CGCG	1 cut(s)	3535
BspLI	GGNNCC	2 cut(s)	916, 1610
BspMAI	CTGCAG	2 cut(s)	2958, 3574
BspMI	ACCTGC	1 cut(s)	1447
BspQI	GCTCTTC	1 cut(s)	2183
BspT104I	TTCGAA	3 cut(s)	1161, 1271, 1640
BspT107I	GGYRCC	1 cut(s)	914
BspTI	CTTAAG	2 cut(s)	407, 2734
BspTNI	GGTCTC	2 cut(s)	1417, 1616
BsrBI	CCGCTC	1 cut(s)	462
BsrDI	GCAATG	2 cut(s)	2625, 3471
BsrFI	RCCGGY	1 cut(s)	3307
BsrGI	TGTACA	2 cut(s)	547, 727
BsrI	ACTGG	7 cut(s)	198, 480, 1413, 1538, 1670, 2279, 2776
BssAI	RCCGGY	1 cut(s)	3307
BssECI	CCNNGG	8 cut(s)	389, 445, 768, 1734, 2096, 2452, 3438, 3576
BssNI	GRCGYC	1 cut(s)	640
BssT1I	CCWWGG	4 cut(s)	445, 1734, 2096, 2452
Bst2UI	CCWGG	5 cut(s)	169, 769, 1213, 1613, 3440
Bst4CI	ACNGT	9 cut(s)	2159, 2289, 2575, 2688, 2888, 3171, 3195, 3520, 3547
Bst6I	CTCTTC	5 cut(s)	112, 1176, 1872, 2183, 3137
BstACI	GRCGYC	1 cut(s)	640
BstAFI	CTTAAG	2 cut(s)	407, 2734
BstAPI	GCANNNNNTGC	1 cut(s)	3381
BstAUI	TGTACA	2 cut(s)	547, 727
BstBI	TTCGAA	3 cut(s)	1161, 1271, 1640
BstC8I	GCNNGC	4 cut(s)	138, 530, 662, 3320
BstDEI	CTNAG	7 cut(s)	125, 1008, 1199, 1899, 3026, 3109, 3476
BstDSI	CCRYGG	1 cut(s)	1734
BstEII	GGTNACC	1 cut(s)	440
BstF5I	GGATG	8 cut(s)	30, 814, 854, 1027, 1063, 1071, 1473, 3007
BstFNI	CGCG	1 cut(s)	3535
BstH2I	RGCGCY	1 cut(s)	1110
BstHHI	GCGC	2 cut(s)	1109, 1683
BstMAI	GTCTC	6 cut(s)	621, 1411, 1417, 1616, 2287, 2564
BstMCI	CGRYCG	1 cut(s)	2359
BstNI	CCWGG	5 cut(s)	169, 769, 1213, 1613, 3440
BstNSI	RCATGY	3 cut(s)	140, 1705, 2412
BstPI	GGTNACC	1 cut(s)	440
BstSCI	CCNGG	9 cut(s)	167, 767, 917, 1211, 1611, 1617, 2516, 3197, 3438
BstSFI	CTRYAG	4 cut(s)	2574, 2769, 2954, 3570
BstSLI	GKGCMC	1 cut(s)	244
BstUI	CGCG	1 cut(s)	3535
BstV1I	GCAGC	4 cut(s)	388, 1983, 2478, 2703
BstV2I	GAAGAC	6 cut(s)	629, 1401, 2130, 2405, 3300, 3376
BstX2I	RGATCY	6 cut(s)	512, 539, 921, 1608, 2950, 3157
BstXI	CCANNNNNNTGG	1 cut(s)	2944
BstYI	RGATCY	6 cut(s)	512, 539, 921, 1608, 2950, 3157
BstZI	CGGCCG	1 cut(s)	2356
BsuI	GTATCC	1 cut(s)	1564
BsuRI	GGCC	6 cut(s)	660, 930, 2358, 2451, 2699, 2775
BtgI	CCRYGG	1 cut(s)	1734
BtsCI	GGATG	8 cut(s)	30, 814, 854, 1027, 1063, 1071, 1473, 3007
BtsI	GCAGTG	1 cut(s)	1763
BtsIMutI	CAGTG	6 cut(s)	487, 504, 1763, 2102, 2155, 2684
BveI	ACCTGC	1 cut(s)	1447
Cac8I	GCNNGC	4 cut(s)	138, 530, 662, 3320
CfoI	GCGC	2 cut(s)	1109, 1683
Cfr10I	RCCGGY	1 cut(s)	3307
Cfr13I	GGNCC	5 cut(s)	7, 658, 1615, 2086, 2510
CseI	GACGC	4 cut(s)	648, 1364, 3381, 3541
Csp6I	GTAC	6 cut(s)	103, 248, 548, 728, 1255, 1918
CviQI	GTAC	6 cut(s)	103, 248, 548, 728, 1255, 1918
DdeI	CTNAG	7 cut(s)	125, 1008, 1199, 1899, 3026, 3109, 3476
DraI	TTTAAA	2 cut(s)	1827, 2712
EaeI	YGGCCR	1 cut(s)	2356
EagI	CGGCCG	1 cut(s)	2356
Eam1104I	CTCTTC	5 cut(s)	112, 1176, 1872, 2183, 3137
EarI	CTCTTC	5 cut(s)	112, 1176, 1872, 2183, 3137
EciI	GGCGGA	1 cut(s)	20
Ecl136II	GAGCTC	1 cut(s)	2435
EclXI	CGGCCG	1 cut(s)	2356
Eco130I	CCWWGG	4 cut(s)	445, 1734, 2096, 2452
Eco24I	GRGCYC	1 cut(s)	2437
Eco31I	GGTCTC	2 cut(s)	1417, 1616
Eco32I	GATATC	1 cut(s)	382
Eco47I	GGWCC	4 cut(s)	7, 1615, 2086, 2510
Eco52I	CGGCCG	1 cut(s)	2356
Eco53kI	GAGCTC	1 cut(s)	2435
Eco57I	CTGAAG	6 cut(s)	641, 1200, 1221, 2873, 3308, 3437
Eco88I	CYCGRG	2 cut(s)	1414, 1928
Eco91I	GGTNACC	1 cut(s)	440
EcoICRI	GAGCTC	1 cut(s)	2435
EcoO65I	GGTNACC	1 cut(s)	440
EcoRII	CCWGG	5 cut(s)	167, 767, 1211, 1611, 3438
EcoRV	GATATC	1 cut(s)	382
EcoT14I	CCWWGG	4 cut(s)	445, 1734, 2096, 2452
EcoT22I	ATGCAT	3 cut(s)	138, 696, 1648
EcoT38I	GRGCYC	1 cut(s)	2437
ErhI	CCWWGG	4 cut(s)	445, 1734, 2096, 2452
Esp3I	CGTCTC	2 cut(s)	621, 2287
FalI	AAGNNNNNCTT	2 cut(s)	2728, 2760
FaqI	GGGAC	3 cut(s)	2533, 2658, 3181
FauI	CCCGC	2 cut(s)	455, 2190
FbaI	TGATCA	3 cut(s)	1800, 2497, 3018
Fnu4HI	GCNGC	7 cut(s)	402, 1862, 1972, 2467, 2717, 3385, 3573
FokI	GGATG	8 cut(s)	17, 821, 861, 1014, 1058, 1070, 1480, 3014
FriOI	GRGCYC	1 cut(s)	2437
Fsp4HI	GCNGC	7 cut(s)	402, 1862, 1972, 2467, 2717, 3385, 3573
FspI	TGCGCA	1 cut(s)	1682
GlaI	GCGC	2 cut(s)	1108, 1682
GluI	GCNGC	7 cut(s)	402, 1862, 1972, 2467, 2717, 3385, 3573
GsaI	CCCAGC	1 cut(s)	1888
GsuI	CTGGAG	1 cut(s)	2774
HaeII	RGCGCY	1 cut(s)	1110
HaeIII	GGCC	6 cut(s)	660, 930, 2358, 2451, 2699, 2775
HapII	CCGG	6 cut(s)	919, 1618, 2186, 2517, 3199, 3308
HgaI	GACGC	4 cut(s)	648, 1364, 3381, 3541
HhaI	GCGC	2 cut(s)	1109, 1683
Hin1I	GRCGYC	1 cut(s)	640
Hin6I	GCGC	2 cut(s)	1107, 1681
HinP1I	GCGC	2 cut(s)	1107, 1681
HincII	GTYRAC	1 cut(s)	1066
HindII	GTYRAC	1 cut(s)	1066
HindIII	AAGCTT	5 cut(s)	711, 3218, 3404, 3419, 3428
HpaII	CCGG	6 cut(s)	919, 1618, 2186, 2517, 3199, 3308
HphI	GGTGA	1 cut(s)	784
Hpy166II	GTNNAC	9 cut(s)	242, 548, 730, 784, 1066, 1436, 1507, 2015, 2742
Hpy8I	GTNNAC	9 cut(s)	242, 548, 730, 784, 1066, 1436, 1507, 2015, 2742
Hpy99I	CGWCG	2 cut(s)	428, 1548
HpyAV	CCTTC	6 cut(s)	68, 799, 1465, 1948, 2237, 2689
HpyCH4III	ACNGT	9 cut(s)	2159, 2289, 2575, 2688, 2888, 3171, 3195, 3520, 3547
HpyCH4IV	ACGT	2 cut(s)	2065, 2671
HpyF3I	CTNAG	7 cut(s)	125, 1008, 1199, 1899, 3026, 3109, 3476
HpySE526I	ACGT	2 cut(s)	2065, 2671
Hsp92I	GRCGYC	1 cut(s)	640
HspAI	GCGC	2 cut(s)	1107, 1681
Ksp22I	TGATCA	3 cut(s)	1800, 2497, 3018
LguI	GCTCTTC	1 cut(s)	2183
LmnI	GCTCC	6 cut(s)	502, 670, 1528, 1785, 1888, 2432
Lsp1109I	GCAGC	4 cut(s)	388, 1983, 2478, 2703
MaeII	ACGT	2 cut(s)	2065, 2671
MbiI	CCGCTC	1 cut(s)	462
MfeI	CAATTG	4 cut(s)	1818, 2891, 2900, 3132
MflI	RGATCY	6 cut(s)	512, 539, 921, 1608, 2950, 3157
MhlI	GDGCHC	5 cut(s)	244, 635, 675, 1533, 2437
MluI	ACGCGT	1 cut(s)	3533
MlyI	GAGTC	6 cut(s)	989, 1376, 1413, 2762, 2899, 3122
MmeI	TCCRAC	5 cut(s)	1035, 1055, 2704, 3068, 3505
Mph1103I	ATGCAT	3 cut(s)	138, 696, 1648
MroXI	GAANNNNTTC	3 cut(s)	419, 1207, 1703
MslI	CAYNNNNRTG	4 cut(s)	689, 770, 1700, 2942
MspCI	CTTAAG	2 cut(s)	407, 2734
MspI	CCGG	6 cut(s)	919, 1618, 2186, 2517, 3199, 3308
MspR9I	CCNGG	9 cut(s)	169, 769, 919, 1213, 1613, 1619, 2518, 3199, 3440
MunI	CAATTG	4 cut(s)	1818, 2891, 2900, 3132
Mva1269I	GAATGC	4 cut(s)	1315, 1648, 1845, 3044
MvaI	CCWGG	5 cut(s)	169, 769, 1213, 1613, 3440
MvnI	CGCG	1 cut(s)	3535
NciI	CCSGG	4 cut(s)	919, 1619, 2518, 3199
NcoI	CCATGG	1 cut(s)	1734
NlaIV	GGNNCC	2 cut(s)	916, 1610
NmuCI	GTSAC	4 cut(s)	64, 2100, 2606, 3514
NsbI	TGCGCA	1 cut(s)	1682
NsiI	ATGCAT	3 cut(s)	138, 696, 1648
NspI	RCATGY	3 cut(s)	140, 1705, 2412
NspV	TTCGAA	3 cut(s)	1161, 1271, 1640
OliI	CACNNNNGTG	1 cut(s)	770
PaeI	GCATGC	1 cut(s)	140
PaeR7I	CTCGAG	1 cut(s)	1414
PciI	ACATGT	2 cut(s)	1701, 2408
PciSI	GCTCTTC	1 cut(s)	2183
PcsI	WCGNNNNNNNCGW	2 cut(s)	102, 2293
PctI	GAATGC	4 cut(s)	1315, 1648, 1845, 3044
PdmI	GAANNNNTTC	3 cut(s)	419, 1207, 1703
PflFI	GACNNNGTC	1 cut(s)	1724
PflMI	CCANNNNNTGG	1 cut(s)	584
PfoI	TCCNGGA	2 cut(s)	1211, 1617
PkrI	GCNGC	7 cut(s)	403, 1863, 1973, 2468, 2718, 3386, 3574
PleI	GAGTC	6 cut(s)	988, 1375, 1413, 2761, 2899, 3121
PpsI	GAGTC	6 cut(s)	988, 1375, 1413, 2761, 2899, 3121
PscI	ACATGT	2 cut(s)	1701, 2408
Psp124BI	GAGCTC	1 cut(s)	2437
Psp6I	CCWGG	5 cut(s)	167, 767, 1211, 1611, 3438
PspEI	GGTNACC	1 cut(s)	440
PspFI	CCCAGC	1 cut(s)	1884
PspGI	CCWGG	5 cut(s)	167, 767, 1211, 1611, 3438
PspN4I	GGNNCC	2 cut(s)	916, 1610
PspPI	GGNCC	5 cut(s)	7, 658, 1615, 2086, 2510
PstI	CTGCAG	2 cut(s)	2958, 3574
PsuI	RGATCY	6 cut(s)	512, 539, 921, 1608, 2950, 3157
PsyI	GACNNNGTC	1 cut(s)	1724
RsaI	GTAC	6 cut(s)	104, 249, 549, 729, 1256, 1919
RsaNI	GTAC	6 cut(s)	103, 248, 548, 728, 1255, 1918
RseI	CAYNNNNRTG	4 cut(s)	689, 770, 1700, 2942
SacI	GAGCTC	1 cut(s)	2437
SapI	GCTCTTC	1 cut(s)	2183
SatI	GCNGC	7 cut(s)	402, 1862, 1972, 2467, 2717, 3385, 3573
Sau96I	GGNCC	5 cut(s)	7, 658, 1615, 2086, 2510
ScaI	AGTACT	1 cut(s)	249
SchI	GAGTC	6 cut(s)	989, 1376, 1413, 2762, 2899, 3122
ScrFI	CCNGG	9 cut(s)	169, 769, 919, 1213, 1613, 1619, 2518, 3199, 3440
SduI	GDGCHC	5 cut(s)	244, 635, 675, 1533, 2437
SfcI	CTRYAG	4 cut(s)	2574, 2769, 2954, 3570
Sfr274I	CTCGAG	1 cut(s)	1414
SfuI	TTCGAA	3 cut(s)	1161, 1271, 1640
SinI	GGWCC	4 cut(s)	7, 1615, 2086, 2510
SlaI	CTCGAG	1 cut(s)	1414
SmiMI	CAYNNNNRTG	4 cut(s)	689, 770, 1700, 2942
SpeI	ACTAGT	1 cut(s)	181
SphI	GCATGC	1 cut(s)	140
SsiI	CCGC	7 cut(s)	5, 418, 462, 1233, 1861, 2183, 3385
SspI	AATATT	3 cut(s)	1637, 3447, 3497
SstI	GAGCTC	1 cut(s)	2437
StyD4I	CCNGG	9 cut(s)	167, 767, 917, 1211, 1611, 1617, 2516, 3197, 3438
StyI	CCWWGG	4 cut(s)	445, 1734, 2096, 2452
TaaI	ACNGT	9 cut(s)	2159, 2289, 2575, 2688, 2888, 3171, 3195, 3520, 3547
TaiI	ACGT	2 cut(s)	2068, 2674
TaqII	GACCGA	1 cut(s)	1851
TatI	WGTACW	4 cut(s)	247, 547, 727, 1917
TauI	GCSGC	2 cut(s)	1864, 3387
TscAI	CASTG	6 cut(s)	487, 504, 1770, 2109, 2162, 2691
TseFI	GTSAC	4 cut(s)	64, 2100, 2606, 3514
TseI	GCWGC	5 cut(s)	401, 1971, 2466, 2716, 3572
Tsp45I	GTSAC	4 cut(s)	64, 2100, 2606, 3514
TspGWI	ACGGA	3 cut(s)	300, 1064, 1532
TspRI	CASTG	6 cut(s)	487, 504, 1770, 2109, 2162, 2691
Tth111I	GACNNNGTC	1 cut(s)	1724
Van91I	CCANNNNNTGG	1 cut(s)	584
Vha464I	CTTAAG	2 cut(s)	407, 2734
VneI	GTGCAC	1 cut(s)	240
VpaK11BI	GGWCC	4 cut(s)	7, 1615, 2086, 2510
XapI	RAATTY	7 cut(s)	331, 971, 1583, 2046, 2707, 2932, 3554
XceI	RCATGY	3 cut(s)	140, 1705, 2412
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1545
XhoI	CTCGAG	1 cut(s)	1414
XmnI	GAANNNNTTC	3 cut(s)	419, 1207, 1703
ZrmI	AGTACT	1 cut(s)	249
Zsp2I	ATGCAT	3 cut(s)	138, 696, 1648

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.